An insecticide composition containing diamide compounds and its application

Abstract

The present invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition containing a diamide compound and its use. The active ingredients of the insecticidal composition include active ingredient A and active ingredient B. Active ingredient A is a compound of Formula I: #imgabs0# (Formula I). ​​Active ingredient B is a diamide compound, including any of flufenacet, tetrazolylpyram, cyclofenacet, chlorfenapyr, or pioxaniliprole. The mass ratio of active ingredient A to active ingredient B is 1:55 to 58:1. The insecticidal composition of the present invention has excellent effects on agricultural, horticultural, and forestry pests.

Description

Technical Field

[0001] The invention belongs to the technical field of insecticide and discloses an insecticide composition containing a diamide compound and application thereof. Background Art

[0002] Diamide insecticides have been a hot topic in the field of insecticide research in recent years. They act on the ryanodine receptors of insects, have a novel and highly effective mechanism of action, have no cross-resistance with traditional pesticides, are safe to non-target organisms, and have good environmental compatibility. They have attracted people's attention and become a hot topic in insecticide research and development.

[0003] However, over time, safety and resistance issues with diamide pesticides have gradually emerged. Based on actual usage, research has found that resistance to diamide pesticides increases significantly after one crop growth cycle, and resistance can increase several dozen times over two years. Therefore, the applicant has formulated the compound of Formula I with a diamide insecticide in an effort to slow the development of resistance, reduce pesticide usage, and minimize trial-and-error costs during the planting process. Summary of the Invention

[0004] To address the aforementioned problems in the prior art, the present invention provides an insecticide composition containing a diamide compound. This insecticide composition exhibits excellent control efficacy against a variety of agricultural, horticultural, and forestry pests, exhibits significant synergistic effects, significantly reduces pesticide usage, and slows the development of pesticide resistance in pests.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: an insecticidal composition containing a diamide compound, wherein the active ingredients of the insecticidal composition include active ingredient A and active ingredient B, and the active ingredient A is a compound of formula I: (Formula I). ​​The active ingredient B is a diamide compound, which includes any one of flufenacet, tetrazolyl thiocarb, cyclofenacet, chlorfenapyr, or pioxaniliprole. The mass ratio of the active ingredient A to the active ingredient B is 1:55 to 58:1, or any value within the above numerical range.

[0006] Furthermore, the active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 42:1, or any value within the above numerical range;

[0007] The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 40:1, or any value within the above numerical range;

[0008] The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 36:1, or any value within the above numerical range;

[0009] The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 45:1, or any value within the above numerical range;

[0010] The active ingredient B is pioxaniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 32:1, or any value within the above numerical range.

[0011] Furthermore, the active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 35:1, or any value within the above numerical range;

[0012] The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 25:1, or any value within the above numerical range;

[0013] The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 25:1, or any value within the above numerical range;

[0014] The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:1, or any value within the above numerical range;

[0015] The active ingredient B is pioxaniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:1, or any value within the above numerical range.

[0016] Furthermore, the active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1, or any value within the above numerical range;

[0017] The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:9 to 20:1, or any value within the above numerical range;

[0018] The active ingredient B is cyclofenac, and the mass ratio of the active ingredient A to the active ingredient B is 1:16 to 15:1, or any value within the above numerical range;

[0019] The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 10:1, or any value within the above numerical range;

[0020] The active ingredient B is pioxaniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 10:1, or any value within the above numerical range.

[0021] Furthermore, the total weight of the insecticide composition is expressed as 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 70% of the total weight of the insecticide composition.

[0022] Furthermore, the insecticide composition contains auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

[0023] Furthermore, the formulation of the insecticide composition is a solid preparation or a liquid preparation;

[0024] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0025] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0026] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is an emulsifiable concentrate, a microemulsion, an aqueous emulsion, a suspension or a dispersible oil suspension.

[0027] The present invention also discloses the insecticide composition as described above for controlling lepidopteran pests and hemiptera pests;

[0028] Furthermore, the Lepidoptera includes, but is not limited to, Plutellaxylostella, diamondback moth, Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (snoctuids), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (soybean looper), Rachiplusia nu, Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides), Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp. (snooper moths), Spodoptera exigua (beet armyworm), Spodoptera fugiperda (fall armyworm), Adoxophyes spp., Adoxophyes orana, Agrotis spp.(cutworm), Agrotis ipsilon (black cutworm), Alabama argillacea, Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anarsia lineatella (peach twig borer), Anomissa bulifera (jute looper), Anticarsia gemma talis (velvet bean pelleter), Archips argyrospila (fruittree leafroller), Archips rosana (rose leaf roller), Argyrotaenia spp. (tortricidmoths), Argyrotaenia citrana, Autographa gamma, Bonagota cranaodes, Borbo cinnara), Caloptilia spp. (leaf miners), Capuareticulana, Carposina niponensis (peach fruit moth), Chilospp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp., Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus, Crambus spp.) (Sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella, Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworms), Earias spp. (Egyptian bollworms). insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbaco moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella, Euxoa auxiliaris (army cutworm), Feltia spp., Gortyna spp.(stemborers), Grapholita molesta (oriental fruit moth), Hedylepta indicate, bean leaf webber, Helicoverpas pp. (spotworms), Helicoverpa paarmigera, Helicoverpa zea, Heliothis spp. (spotworms), Heliothis virescens, tobacco budworm, Hellula undalis (cabbage webworm), Indarbela spp., root borers, Keiferia lycopersicella, (tomato pinworm), Leucinodes orbonalis (eggplant fruit borer). borer), Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp. (southworm), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.(tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis, Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis (small tomato borer), Operophthera brumata (winter moth), Ostrinia nubilalis (European corn borer), Pandemis cerasana, common currant tortrix, Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma species ( spp. (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp. (leafminers), Pieris rapae (imported cabbageworm), Plathypena scabra, Plodia interpunctella (Indian mealmoth), Spodoptera oridania (southern armyworm), Synanthedon spp.) (root borers), Thecla basilides, Thermisiagemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (rape worm), Tuta absoluta, Yponomeuta spp., Zeuzera coffeae (red branch borer), and Zeuzera pyrina (leopard moth).

[0029] The Hemiptera pests include, but are not limited to, stink bugs: Acrosternum hilare (green stink bug), Blissus leucopterus (chinch bug), Calocoris norvegicus (potato mirid), Cimex hemipterus (tropical bed bug), Cimex lectularius (bed hug), Daghertus fasciatus, Dichelops furcatus, Dysdercus suturellus (cotton stainer), Edessa meditabunda, Eurygaster maura (cereal bug), Euschistus heros, Euschistus servus (brown stink bug), Helopeltis antonii, Helopeltis theivora (tea blight plant bug), Lagynotomus spp. (stink bugs), Leptocorisa oratorius, Leptocorisa varicornis, Lygus spp. (plant bugs), Lygus hesperus (western tarnished plant bug), Maconellicoccus hirsutus, Neurocolpus longirostris, Nezara viridula (southern green stink bug), PhyLocoris spp.) (lygus), Phytocoris californicus, Phytocoris relativus, Piezodorus guildingi, Poecilocapsus lineatus (fourlined plant bug), Psallus vaccinicola, Pseudacysta perseae, Scaptocoris castanea, and Triatoma spp. (bloodsucking conenoses / kissing bugs), Acrythosiphonpisum (pea aphid), Adelges spp. (adelgids), Aleurodes proletella (cabbage whitefly), Aleurodicus disperses, Aleurothrixus flccosus (woolly whitefly) (whitefly), Aluacaspis spp., Amrasca bigutella bigutella, Aphrophora spp. (leahopper), Aonidiella aurantii (California redscale), Aphis spp. (aphids), Aphis gossypii (cotton aphid), Aphis pomi (apple aphid), Aulacorthitm solani (foxglove aphid), Bemisia spp. (whitefly), Bemisia argentifolii, Bemisia tabaci (sweetpotato whitefly), Brachycolus noxius (Russian aphid), aphid), Brachycorynclia asparagi (asparagus aphid), Brevennia rehi, Brevicoryne brassicae (cabbage aphid), Ceroplastes spp.) (scale), Ceroplastes rubens (red wax scale), Chionaspis spp. (scale), Chrysomphalus spp. (scale), Coccus spp. (scale), Dysaphis plantaginea (rosy apple aphid), Empoasca spp. (leafhopper), Eriosomalanigerum (woolly apple aphid), Icerya purchasi (cottony cushion scale), Idioscopus nitidulus (mango leafhopper), Laodelphax striatellus (smaller brown planthopper), Lepidosaphes spp., Macrosiphum spp.), Macrosiphum euphorbiae (potato aphid), Macrosiphum granarium (English grain aphid), Macrosiphum rosae (rose aphid), Macrosteles quadrilineatus (asterleafhopper), Mahanarva frimbiolata, Metopolophium dirhodum (rose grain aphid), Midis longicornis, Myzus persicae (green peach aphid), Nephotettix spp.) (leafhopper), Nephotettix cinctipes (green leafhopper), Nilaparvata lugens (brown planthopper), Parlatoria pergandii (chaff scale), Parlatoria ziziphi (ebonyscale), Peregrinus maidis (corndelphacid), Philaenus spp. (blowing insects), Phylloxera vitifoliae (grape phylloxera), Physokermes piceae (spruce budscale), Planococcus spp. (mealybugs), Pseudococcus spp. (mealybugs), Pseudococcus brcvipcs (pinc apple mealybugs) mcalybug), Quadraspidiotus perniciosus (San Jose scale), Rhapalosiphum spp. (aphids), Rhapalosiphum maida (corn leaf aphid), Rhapalosiphum padi (oatbird-cherry aphid), Saissetia spp. (scale), Saissetiaoleae (black scale), Schizaphis graminum (wheat aphid), Sitobion avenge (British wheat aphid), Sogatella furcifera (white-backed planthopper), Therioaphis spp. (aphids), Toumeyella spp. (scale), Toxoptera spp. (aphids), Trialeurodes spp.) (whitefly), Trialeurodes vaporariorum (greenhouse whitefly), Trialeurodes abutiloneus (bandedwing whitefly), Unaspis spp. (scale), Unaspis yanonensis (arrowhead scale), and Zulia entreriana.

[0030] In particular, the insecticidal composition of the present invention has excellent control effects on diamondback moth, beet armyworm, rice borer, whitefly, rice planthopper, aphid, etc., and exhibits a synergistic effect within the above-mentioned mass ratio range;

[0031] Furthermore, the above-mentioned insecticide composition or its formulation is applied to the pests to be controlled or the medium in which they grow.

[0032] In order to obtain the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0033] The beneficial effects of the present invention are as follows:

[0034] (1) The insecticidal composition of the present invention rationally compounds insecticidal compounds with different mechanisms of action, and has a significant synergistic effect;

[0035] (2) The insecticidal composition of the present invention has excellent control effects on Hemiptera and Lepidoptera pests in agriculture, horticulture, and forestry, and expands the insecticidal spectrum of a single dose;

[0036] (3) The insecticide composition of the present invention reduces the dosage of pesticides, reduces agricultural production costs, and is safe for crops and the environment. DETAILED DESCRIPTION

[0037] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0038] Preparation example:

[0039] Preparation Example 1: 32% Formula I compound·flufenacil wettable powder (1:3)

[0040] Formula: 8% compound of formula I, 24% flufenac, 5% sodium salt of polycarboxylate, 3% naphthalenesulfonate formaldehyde condensate, 2% pulverized powder BX, 5% white carbon black, and kaolin to make up the balance;

[0041] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0042] Preparation Example 2: 24% Formula I compound·flufenacil suspension concentrate (7:1)

[0043] Formula: 21% compound of formula I, 3% flufenac, 1% fatty alcohol polyoxyethylene ether, 2% polyoxyethylene sorbitan monooleate, 3% styrylphenol polyoxyethylene phosphate, 1% sodium lignin sulfonate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% sodium sorbate, 0.5% silicone oil, and deionized water to make up the balance;

[0044] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0045] Preparation Example 3: 30% Formula I compound·tetrazolylcarboxamide water dispersible granules (5:1)

[0046] Formula: 25% compound of formula I, 5% tetrazolyl amide, 8% lignin sulfonate, 6% sodium salt of polycarboxylate, 2% sodium lauryl sulfate, 5% white carbon black, 20% starch, and kaolin makes up the balance.

[0047] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and sieving are carried out to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0048] Preparation Example 4: 21% Formula I compound·tetrazolyl tetrazolamide suspension (1:6)

[0049] Formula: 3% compound of formula I, 18% tetrazolyl thiazolin, 2% isomeric tridecanol polyoxyethylene ether, 4% anhydrous sorbitan oleate polyoxyethylene ether, 2% styrenated phenol polyoxyethylene ether phosphate, 2% sodium polycarboxylate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0050] Preparation method: same as Preparation Example 2.

[0051] Preparation Example 5: 25% Formula I compound·Cyclofenac dispersible oil suspension (3:2)

[0052] Formula: 15% compound of formula I, 10% cyclobromofenapyr, 5% glycerol fatty acid ester polyoxyethylene ether, 10% fatty amine polyoxyethylene ether, 1% calcium dodecylbenzenesulfonate, 3% castor oil polyoxyethylene ether, 1% polycarboxylic acid sodium salt, 2% naphthalenesulfonate formaldehyde condensate, 0.5% organic bentonite, and methyl oleate makes up the balance;

[0053] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0054] Preparation Example 6: 18% Formula I compound·Cyclofenac suspension (8:1)

[0055] Formula: 16% compound of formula I, 2% cyclobromofenapyr, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 1% naphthalenesulfonate formaldehyde condensate, 2% arylphenol polyoxyethylene ether phosphate, 3% alkylaryl polyoxyethylene ether polyoxypropylene ether, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.2% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0056] Preparation method: same as Preparation Example 2.

[0057] Preparation Example 7: 14% Formula I compound·Chlorantraniliprole suspension (2:5)

[0058] Formula: 4% compound of formula I, 10% chlorantraniliprole, 5% castor oil polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether phosphate, 1% sodium salt of polycarboxylate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.01% isothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0059] Preparation method: same as Preparation Example 2.

[0060] Preparation Example 8: 33% Formula I compound·Chlorantraniliprole water dispersible granules (10:1)

[0061] Formula: 30% compound of formula I, 3% chlorantraniliprole, 8% sodium lignin sulfonate, 3% flavonoids BX, 8% naphthalenesulfonate formaldehyde condensate, 8% ammonium sulfate, and kaolin makes up the balance.

[0062] Preparation method: Same as Preparation Example 3.

[0063] Preparation Example 9: 0.6% Formula I compound·pioxaniliprole aqueous emulsion (5:1)

[0064] Formula: 0.6% compound of formula I, 0.1% pioxaniliprole, 3% sorbitan oleate polyoxyethylene ether, 5% tristyrylphenol ethoxylate phosphate, 15% cyclohexanone, 0.25% xanthan gum, 5% ethylene glycol, 1% urea, 0.2% sodium benzoate, 0.02% silicone defoaming agent, and deionized water to make up the balance;

[0065] Preparation method: After completely dissolving the active ingredient in the solvent according to the formula ratio, add the emulsifier to form the oil phase; stir the dispersant, antifreeze, deionized water, etc. to form the water phase; add the oil phase to the water phase and stir evenly, shear at high speed until the particle size meets the requirements, add the defoaming agent and stir evenly to obtain the water emulsion product.

[0066] Preparation Example 10: 25% Formula I compound·pioxaniliprole suspension (1:4)

[0067] Formula: 5% compound of formula I, 20% pioxaniliprole, 1% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% sodium salt of polycarboxylate, 3% fatty alcohol polyoxyethylene ether sulfate, 2% ethylene glycol oxyethylene polyoxypropylene ether, 1% fumed silica, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 5% propylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0068] Preparation method: same as Preparation Example 2.

[0069] Indoor biological activity test

[0070] Example 1: Bioactivity assay on rice stem borer

[0071] Test basis: The test refers to NY / T-1154.10-2008 "Guidelines for Indoor Biological Tests of Pesticides - Insecticides Part 10: Artificial Diet Mixture Method" and NY / T 1154.7-2006 "Guidelines for Indoor Biological Tests of Pesticides - Insecticides Part 7: Determination of Combined Effects of Mixtures".

[0072] Test target: Rice stem borer (Chilo suppressalis), third instar larvae.

[0073] Test agents: compound of formula I, flufenac, tetrazobactam, cyclofenac, chlorfenapyr, and pioxaniliprole technical.

[0074] Test Method: Select standard test insects reared indoors and in consistent physiological condition. Prepare a stock solution of the original drug with an appropriate solvent and prepare five concentrations using the isocratic method. Take an appropriate amount of the diluted solution and evenly mix it into the prepared artificial diet. Pour the solution into 12-well plates while still hot and allow to cool until ready to use. One test insect is seeded into each well. Each treatment is replicated four times, with 20 insects per replicate. A treatment without the drug (but with the corresponding solvent) serves as a blank control.

[0075] Rearing and observation: The treated test insects were reared and observed under the conditions of temperature of (25±1)℃, relative humidity of 60% to 80%, and photoperiod L:D=(16:8)h.

[0076] Investigation: Check the mortality of test insects 48 hours after treatment and record the total number of insects and the number of dead insects respectively.

[0077] Calculation method:

[0078] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0079]

[0080] Where:

[0081] P——mortality rate, in percentage (%);

[0082] K——indicates the number of dead insects, the unit is head;

[0083] N——represents the total number of insects processed, in heads.

[0084]

[0085] Where:

[0086] P1——adjusted mortality rate, in percentage (%);

[0087] P t ——Treatment mortality rate, expressed in percentage (%);

[0088] P0 - blank control mortality rate, in percentage (%).

[0089] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0090] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0091] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0092]

[0093] Where:

[0094] ATI - measured toxicity index of mixture;

[0095] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0096] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0097] TTI=TI A ×P A +TI B ×P B

[0098] Where:

[0099] TTI – Theoretical Toxicity Index of Mixtures;

[0100] TI A ——Agent toxicity index;

[0101] P A ——The percentage of agent A in the mixture, in percentage (%);

[0102] TI B ——Toxicity index of agent B;

[0103] P B ——The percentage of agent B in the mixture, in percentage (%).

[0104]

[0105] Where:

[0106] CTC – Co-toxicity coefficient;

[0107] ATI - measured toxicity index of mixture;

[0108] TTI - Theoretical Toxicity Index of Mixture.

[0109] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0110] The indoor test results are shown in the table below:

[0111] Table 1 Results of indoor biological activity test of compound of formula I and flufenacet against Chilo suppressalis

[0112]

[0113] Indoor test results (Table 1) show that compounding the formula I compound with flubendiamide in an appropriate mass ratio exhibits excellent control effects against the rice stem borer. When the mass ratio of the formula I compound to flubendiamide is 1:25 to 35:1, the co-toxicity coefficient is greater than 120, exhibiting a synergistic effect against the rice stem borer. When the mass ratio of the formula I compound to flubendiamide is 1:20 to 30:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the formula I compound to flubendiamide is 1:6 to 15:1, the co-toxicity coefficient is greater than 150, indicating a significant synergistic effect.

[0114] Table 2 Indoor biological activity test results of compound of formula I and tetrazolyl thiamethoxam against rice stem borer

[0115]

[0116]

[0117] Laboratory test results (Table 2) show that the compound of Formula I, when combined with tetrazolin at an appropriate mass ratio, exhibits excellent control efficacy against the rice stem borer. When the mass ratio of the compound of Formula I to tetrazolin is 1:20 to 40:1, the co-toxicity coefficient is greater than 120, demonstrating a synergistic effect against the rice stem borer. When the mass ratio of the compound of Formula I to tetrazolin is 1:10 to 30:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to tetrazolin is 1:5 to 10:1, the co-toxicity coefficient is greater than 150, demonstrating a significant synergistic effect.

[0118] Table 3 Indoor biological activity test results of compound of formula I and cyclobromofenapyr against Chilo suppressalis

[0119]

[0120] Laboratory test results (Table 3) show that the compound of Formula I, when combined with cyclofenamic acid at appropriate mass ratios, exhibits excellent control efficacy against the rice stem borer. The mass ratios of Formula I to cyclofenamic acid range from 1:32 to 25:1, with a co-toxicity coefficient greater than 120, exhibiting a synergistic effect against the rice stem borer. The mass ratios of Formula I to cyclofenamic acid range from 1:16 to 15:1, with a co-toxicity coefficient greater than 140, exhibiting a significant synergistic effect. The mass ratios of Formula I to cyclofenamic acid range from 1:8 to 4:1, with a co-toxicity coefficient greater than 150, exhibiting a significant synergistic effect.

[0121] Table 4 Indoor biological activity test results of compound of formula I and chlorantraniliprole against Chilo suppressalis

[0122]

[0123]

[0124] Laboratory test results (Table 4) show that the compound of Formula I, when combined with chlorantraniliprole in appropriate mass ratios, exhibits excellent control efficacy against the rice stem borer. When the mass ratio of the compound of Formula I to chlorantraniliprole is 1:35 to 20:1, the co-toxicity coefficient is greater than 120, demonstrating a synergistic effect against the rice stem borer. When the mass ratio of the compound of Formula I to chlorantraniliprole is 1:20 to 10:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to chlorantraniliprole is 1:5 to 1:1, the co-toxicity coefficient is greater than 150, demonstrating a significant synergistic effect.

[0125] Table 5 Indoor bioactivity test results of compound of formula I combined with pioxaniliprole against Chilo suppressalis

[0126]

[0127] Laboratory test results (Table 5) show that the compound of Formula I, when combined with pioxaniliprole in appropriate mass ratios, exhibits excellent control efficacy against the rice stem borer. When the mass ratio of the compound of Formula I to pioxaniliprole is 1:20 to 25:1, the co-toxicity coefficient is greater than 120, demonstrating a synergistic effect against the stem borer. When the mass ratio of the compound of Formula I to pioxaniliprole is 1:15 to 20:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to pioxaniliprole is 1:15 to 10:1, the co-toxicity coefficient is greater than 150, demonstrating a significant synergistic effect.

[0128] Example 2: Biological activity assay against aphids

[0129] Test basis: Reference test criteria: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 14: Leaf Dip Method" and NY / T 1154.7-2006 "Guidelines for Indoor Bioassay Tests of Pesticides Insecticides Part 7: Determination of Combined Action of Mixtures".

[0130] Test agents: compound of formula I, flufenac, tetrazobactam, cyclofenac, chlorfenapyr, and pioxaniliprole technical.

[0131] The test target is Macrosiphum euphorbiae, and the test target is a population that has been continuously reared and reproduced for multiple generations indoors.

[0132] Preparation of medicine: First dissolve the original medicine in a suitable solvent to prepare a high-concentration mother solution, and then dilute the above single doses and mixtures into 5 series of mass concentrations with an aqueous solution containing 0.1% Tween 80.

[0133] Test method: Select fresh potato leaves that are basically consistent in size, leaf color and other indicators and have not been treated with any pesticides. Wash and dry them, then immerse them in the test solution. Take them out after 10 seconds and place them in an insect box covered with moisturizing filter paper.

[0134] Wingless adult aphids of similar size and vigor reared indoors were inoculated onto treated leaves. Each replicate contained 20 aphids. Each treatment and control were replicated four times. A blank control containing the same solvent without the aphid was used. After treatment, each box was placed in an artificial climate incubator at (26±1)°C, relative humidity (60±5)%, and a 16-hour light cycle.

[0135] Experimental investigation: Observe the test insects 48 hours after treatment. Use a brush to gently touch the insects. Insects without spontaneous reactions are considered dead. Record the number of dead insects.

[0136] Data statistics and analysis:

[0137] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0138]

[0139] Where:

[0140] P——mortality rate, in percentage (%);

[0141] K——indicates the number of dead insects, the unit is head;

[0142] N——represents the total number of insects processed, in heads.

[0143]

[0144] Where:

[0145] P1——adjusted mortality rate, in percentage (%);

[0146] P t ——Treatment mortality rate, expressed in percentage (%);

[0147] P0 - blank control mortality rate, in percentage (%).

[0148] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0149] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0150] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0151]

[0152] Where:

[0153] ATI - measured toxicity index of mixture;

[0154] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0155] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0156] TTI=TI A ×P A +TI B ×P B

[0157] Where:

[0158] TTI – Theoretical Toxicity Index of Mixtures;

[0159] TI A ——Agent toxicity index;

[0160] P A ——The percentage of agent A in the mixture, in percentage (%);

[0161] TI B ——Toxicity index of agent B;

[0162] P B ——The percentage of agent B in the mixture, in percentage (%).

[0163]

[0164] Where:

[0165] CTC – Co-toxicity coefficient;

[0166] ATI - measured toxicity index of mixture;

[0167] TTI - Theoretical Toxicity Index of Mixture.

[0168] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. The results of the laboratory test are shown in the table below:

[0169] Table 6 Results of indoor biological activity test on aphids by combining compound of formula I and flufenacet

[0170]

[0171] The results of the indoor tests (Table 6) show that the compound of Formula I and flufenacet, when combined in an appropriate mass ratio, exhibit excellent control effects against aphids. The mass ratio of the compound of Formula I to flufenacet was 1:36 to 42:1, with a co-toxicity coefficient greater than 120, exhibiting a synergistic effect against aphids. The mass ratio of the compound of Formula I to flufenacet was 1:18 to 15:1, with a co-toxicity coefficient greater than 130, exhibiting a significant synergistic effect. The mass ratio of the compound of Formula I to flufenacet was 1:3 to 7:1, with a co-toxicity coefficient greater than 150, exhibiting a significant synergistic effect.

[0172] Table 7 Results of indoor biological activity test on aphids by combining compounds of formula I with tetrazolylpyram

[0173]

[0174] The results of the indoor tests (Table 7) show that the compound of Formula I and tetrazolin, when combined in an appropriate mass ratio, exhibit excellent control effects against aphids. The mass ratio of the compound of Formula I to tetrazolin is 1:18 to 25:1, with a co-toxicity coefficient greater than 120, exhibiting a synergistic effect against aphids. The mass ratio of the compound of Formula I to tetrazolin is 1:9 to 20:1, with a co-toxicity coefficient greater than 130, exhibiting a significant synergistic effect. The mass ratio of the compound of Formula I to tetrazolin is 1:6 to 6:1, with a co-toxicity coefficient greater than 150, exhibiting a significant synergistic effect.

[0175] Table 8 Results of indoor biological activity test on aphids by combining compound of formula I and bromofenapyr

[0176]

[0177] The results of the indoor tests (Table 8) show that the compound of Formula I and cyclofenamic acid, when combined in appropriate mass ratios, exhibit excellent control effects against aphids. The mass ratio of the compound of Formula I to cyclofenamic acid was 1:40 to 36:1, with a co-toxicity coefficient greater than 120, exhibiting a synergistic effect against aphids. The mass ratio of the compound of Formula I to cyclofenamic acid was 1:30 to 18:1, with a co-toxicity coefficient greater than 130, exhibiting a significant synergistic effect. The mass ratio of the compound of Formula I to cyclofenamic acid was 1:15 to 2:1, with a co-toxicity coefficient greater than 150, exhibiting a significant synergistic effect.

[0178] Table 9 Results of indoor biological activity test on aphids of compound I and chlorantraniliprole

[0179]

[0180] The results of the indoor tests (Table 9) show that the compound of Formula I, when combined with chlorantraniliprole in an appropriate mass ratio, exhibits excellent control effects against aphids. When the mass ratio of the compound of Formula I to chlorantraniliprole is 1:35-45:1, the co-toxicity coefficient is greater than 120, demonstrating a synergistic effect against aphids. When the mass ratio of the compound of Formula I to chlorantraniliprole is 1:20-35:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to chlorantraniliprole is 1:5-20:1, the co-toxicity coefficient is greater than 150, demonstrating a significant synergistic effect.

[0181] Table 10 Results of indoor biological activity test on aphids using the compound of formula I and pioxaniliprole

[0182]

[0183] Laboratory test results (Table 10) show that the compound of Formula I, when combined with pioxaniliprole in appropriate mass ratios, exhibits excellent control efficacy against aphids. When the mass ratio of the compound of Formula I to pioxaniliprole is 1:45 to 32:1, the co-toxicity coefficient is greater than 120, demonstrating a synergistic effect against aphids. When the mass ratio of the compound of Formula I to pioxaniliprole is 1:32 to 8:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to pioxaniliprole is 1:8 to 4:1, the co-toxicity coefficient is greater than 150, demonstrating a significant synergistic effect.

[0184] Field efficacy test:

[0185] Example 3: Field efficacy test for controlling aphids

[0186] Test subjects: potato aphid;

[0187] The experiment was conducted at the Shouguang Vegetable Planting Base in Weifang City, Shandong Province. The soil fertility was above average, irrigation conditions were good, and all plots were maintained under uniform cultivation and management conditions.

[0188] Experimental method: Each experimental plot is arranged in random blocks, with protection rows set between adjacent plots. Each plot is 20m2 in area. 2 Each treatment was repeated 4 times. The experiment was carried out on August 28, 2023, using the conventional spray method, with a liquid volume of 45L per mu, and a total of one application during the entire experiment.

[0189] Survey Methods: The base population was surveyed before treatment, and the number of remaining insects was surveyed three times three days after treatment. Five random sampling points were selected from each plot, with three plants surveyed at each point. Aphids were marked on one leaf per plant, and the number of live aphids was recorded.

[0190] Calculation method of drug efficacy:

[0191]

[0192] During the experiment, potatoes in each treatment plot grew well and no phytotoxicity was observed in any treatment.

[0193] The test results are shown in the following table:

[0194] Table 11 Results of field efficacy tests on aphid control

[0195]

[0196]

[0197] Field efficacy tests show that the insecticidal compositions of the present invention have a good control effect on potato aphids. Three days after application, the control efficacy of the 32% Formula I compound flufenacet wettable powder (1:3), the 30% Formula I compound tetrazolam water-dispersible granules (5:1), the 0.6% Formula I compound pioxaniliprole water emulsion (5:1), the 25% Formula I compound cyclofenamic acid dispersible oil suspension (3:2), and the 33% Formula I compound chlorfenapyr water-dispersible granules (10:1) were 95.25%, 94.90%, 85.16%, 87.74%, and 89.29%, respectively, demonstrating good rapid efficacy. Seven days after application, the efficacy of each composite formulation was 98.29%, 97.54%, 90.83%, 92.10%, and 93.04%, respectively, demonstrating a long-lasting effect.

[0198] Example 4: Experiment on efficacy of drugs for controlling rice stem borer

[0199] Test basis: The test refers to GB / T 17980.1-2000 "Guidelines for field efficacy tests of pesticides (I) Insecticides for controlling rice lepidopteran borers".

[0200] The experimental site is located in Xizhenggang Village, Yandian Township, Feixi County, Anhui Province. The experimental site is flat, the soil is a nutrient-rich yellow-white sandy mud field, and the cultivation and management conditions are good.

[0201] Experimental method: The experiment was conducted with 12 treatments and 4 replicates, and the area of ​​each plot was 30m 2 The plots were randomly arranged in groups, with a 0.5m isolation zone between the plots, and the growth conditions of the plants in each plot were consistent.

[0202] The experiment was conducted on August 4, 2023, during the period from the peak hatching period of the second generation of Chilo suppressalis eggs to the occurrence period of young larvae. A backpack electric sprayer was used to add 600L / hm2 of water. 2 , the rice stems and leaves were sprayed once during the entire test process, and the blank control area was sprayed with an equal amount of water.

[0203] Survey method: A survey was conducted once in the blank control plots, when the infestation of C. suppressalis was established, on September 3, 2023. The parallel line jump method was used for sampling, with 25 rice clumps surveyed in each plot, and the dead heart rate was calculated.

[0204] Calculation method of drug efficacy:

[0205]

[0206] The results of the field efficacy test are shown below:

[0207] Table 12 Results of the efficacy test on controlling rice stem borer

[0208]

[0209]

[0210] Safety investigation: During the field trial, no pesticide damage occurred in any treatment group, and no adverse effects were found on the surrounding environment and other beneficial organisms in the test area, indicating that the test agents were safe for rice growth at the doses provided.

[0211] The results (see Table 12) showed that the rapid-acting and long-lasting effectiveness of the 25% Formula I compound pioxaniliprole suspension concentrate (1:4), the 30% Formula I compound pyraclostrobin water-dispersible granules (5:1), the 24% Formula I compound flufenamid suspension concentrate (7:1), the 25% Formula I compound cyprodinil dispersible oil suspension concentrate (3:2), and the 14% Formula I compound chlorantraniliprole suspension concentrate (2:5) were significantly superior to the other agents in controlling the rice stem borer. The 30% Formula I compound pyraclostrobin water-dispersible granules (5:1) had the highest efficacy, at 96.84%.

[0212] In summary, through indoor toxicity assays and field efficacy tests, it can be seen that the insecticidal composition of the present invention has a good control effect on Lepidoptera and Hemiptera pests, is safe for target crops, has significant control effects, and is superior to a single agent in delaying the development of drug resistance and prolonging the effectiveness.

[0213] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An insecticide composition containing a diamide compound, characterized in that: The active ingredients of the insecticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula I: (Formula I), the active ingredient B is a bisamide compound, and the bisamide compound includes any one of flufenacet, tetrazolam, cyclofenacet, chlorantraniliprole or pioxaniliprole; The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 35:1; The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:18 to 25:1; The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 25:1; The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 20:1; The active ingredient B is pioxaniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:

1.

2. The insecticidal composition according to claim 1, characterized in that The active ingredient B is flubendiamide, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1; The active ingredient B is tetrazolin, and the mass ratio of the active ingredient A to the active ingredient B is 1:9 to 20:1; The active ingredient B is cyclobromofenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:16 to 15:1; The active ingredient B is chlorantraniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 10:1; The active ingredient B is pioxaniliprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 10:

1.

3. The insecticidal composition according to claim 1, characterized in that The total weight of the insecticide composition is expressed as 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 70% of the total weight of the insecticide composition.

4. The insecticidal composition according to claim 1, characterized in that The insecticide composition contains auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The insecticidal composition according to claim 1, characterized in that The formulation of the insecticide composition is a solid preparation or a liquid preparation.

6. The insecticidal composition according to claim 5, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is an emulsifiable concentrate, a microemulsion, an aqueous emulsion, a suspension or a dispersible oil suspension.

7. The insecticidal composition according to any one of claims 1 to 6 is used for controlling lepidopteran pests and hemiptera pests.

8. Use according to claim 7, characterized in that The lepidopteran pests are rice stem borers, and the hemiptera pests are aphids.

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