Preparation method and application of artemisia rupestris n-butyl alcohol effective part extract

Abstract

The invention relates to a preparation method and application of an artemisia rupestris n-butyl alcohol effective part extract. The method comprises the following steps: crushing the whole herb of artemisia rupestris, adding water, uniformly mixing, carrying out reflux extraction, combining, filtering, carrying out reduced-pressure recovery and concentration to obtain an artemisia rupestris water extract, extracting with n-butyl alcohol, combining extract liquor, carrying out reduced-pressure concentration, and carrying out vacuum drying to obtain the effective part extract of the artemisia rupestris n-butyl alcohol. The extract obtained by the method provided by the invention has three experiments of influence on gastric acid, gastric secretion volume and pepsin activity of a rat with functional dyspepsia caused by a composite molding method, influence on gastrointestinal function of a mouse with functional dyspepsia caused by L-arginine, influence on gastrointestinal motility of a normal mouse and the like; it is proved that the artemisia rupestris n-butyl alcohol effective part extract has activity in the aspect of effectively relieving functional dyspepsia and gastrointestinal motility disorder, and a new way is further provided for the aspect of functional dyspepsia treating medicine through the extract.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and more specifically to a preparation method and application of an extract of n-butanol effective part of Artemisia affine Background Art

[0002] Functional dyspepsia (FD) is a common chronic non-organic gastrointestinal disease and a common disease of the digestive system. It has clinical symptoms of postprandial discomfort, early satiety, upper abdominal pain or burning. The incidence of FD in Western countries is about 10%-40%, and the incidence in Asian countries is 5%-30%. The disease has a long course and is prone to recurrence. The incidence is high and has an increasing trend year by year, which seriously affects the quality of life of patients. Modern medicine has no specific means to cure the disease. Generally, symptomatic treatments such as prokinetic drugs, acid inhibitors and digestive drugs are used. The prescriptions involved in traditional Chinese medicine intervention for FD are mainly based on strengthening the spleen, soothing the liver, and promoting qi, such as Zhishi, Xiangsha Liujunzi Decoction, Sinisan, etc. Therefore, the development of efficient and less adverse reactions of functional dyspepsia drugs is an urgent problem to be solved by technicians in this field.

[0003] Artemisia rupestris L. is the whole herb of Artemisia rupestris L., a plant of the genus Artemisia in the Asteraceae family. The whole herb is used as medicine. It is mainly distributed in Xinjiang in my country. Its Uyghur name is Yizihu Aimani. It grows in areas with higher altitudes. It has been used by the people in Xinjiang for a long time and has significant medicinal effects. It is now included in the "National Drug Standards of the Ministry of Health of the People's Republic of China (Uyghur Medicine Volume)" and "Uyghur Medicine Records". As a characteristic ethnic medicine in Xinjiang, Artemisia rupestris has multiple effects such as dispelling wind and activating blood circulation, strengthening the stomach and digestion, dispersing blood stasis and reducing swelling, clearing away heat and detoxifying, anti-allergic, anti-tumor, anti-vomiting, hemostasis, and detoxification of snake venom. It is clinically used to treat indigestion, colds and fevers, hepatitis, urticaria, allergic diseases, etc. At present, the research, development and utilization of Artemisia rupestris in China have a history of nearly 40 years. The chemical components of this medicinal material include sesquiterpenes, flavonoids, organic acids, coumarins, alkaloids, amino acids, polysaccharides, volatile oils and other components, among which sesquiterpenes and flavonoids are relatively rich. Previous literature has shown that the water extract of Artemisia striata can effectively promote the gastric emptying rate and small intestinal propulsion rate of mice. Therefore, this application intends to further extract and separate the water extract to obtain the effective part extract of Artemisia striata n-butanol, and verify the therapeutic effect on functional dyspepsia. Summary of the invention

[0004] The present invention aims to provide a preparation method and application of an effective part extract of Artemisia striata with n-butanol. The method comprises crushing the whole herb of Artemisia striata, adding water to mix evenly, performing reflux extraction, combining and filtering, and then recovering and concentrating under reduced pressure to obtain an Artemisia striata water extract, and then extracting with n-butanol, combining the extracts, concentrating under reduced pressure, and vacuum drying to obtain an effective part extract of Artemisia striata with n-butanol. The extract obtained by the method of the present invention is applied to a functional dyspepsia rat model caused by a composite modeling method to observe its effects on the gastric juice secretion, gastric acid and pepsin activity of the rats, and the changes in the levels of motilin (MTL), gastrin (GAS) and serotonin (5-HT) in the rat serum; it is applied to a functional dyspepsia mouse model caused by L-arginine to observe its effects on the gastric emptying rate and small intestinal propulsion rate of the functional dyspepsia model mice; it is applied to the gastrointestinal motility of normal mice, and its effects on the gastric emptying rate and small intestinal propulsion rate of normal mice are observed; by determining: the efficacy of the extract of the effective part of Artemisia annua n-butanol in treating functional dyspepsia is further verified, laying a theoretical foundation for further developing and utilizing the role of the extract of the effective part of Artemisia annua n-butanol in treating functional dyspepsia.

[0005] The method for preparing the extract of the effective part of Artemisia argyi n-butanol of the present invention is carried out according to the following steps:

[0006] a. After crushing the whole herb of Artemisia striata, add 10 mL of water to every 1 g of the crushed Artemisia striata and mix well to obtain a mixed solution;

[0007] b. Reflux the mixed solution obtained in step a for 3 times, each time for 1 hour, combine the reflux extracts and filter to obtain a filtrate;

[0008] c. recovering the filtrate obtained in step b under reduced pressure and concentrating it to obtain an Artemisia striata extract;

[0009] d. Disperse the Artemisia striata extract obtained in step c in water to obtain a suspension, extract it with n-butanol for 3 times, combine the extracts, concentrate under reduced pressure, and dry them in vacuum to obtain the n-butanol effective part extract of Artemisia striata.

[0010] Application of the Artemisia affine n-butanol effective part extract obtained by the method in preparing medicine for treating functional dyspepsia.

[0011] The extract of the effective part of Artemisia annua obtained by the method of the present invention is used in the preparation of functional dyspepsia to increase the gastric juice secretion of rats with functional dyspepsia model; increase the pepsin activity of rats with functional dyspepsia model; reduce the total acidity of gastric contents in rats with functional dyspepsia model; increase the MTL level in the serum of rats with functional dyspepsia model; increase the gastrin (GAS) level in the serum of rats with functional dyspepsia model; reduce the 5-HT level in the serum of rats with functional dyspepsia model; increase the gastric emptying rate of mice with functional dyspepsia model induced by L-arginine; increase the small intestinal propulsion rate of mice with functional dyspepsia model induced by L-arginine; increase the gastric emptying rate of normal mice; and increase the small intestinal propulsion rate of normal mice. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The results of light microscopy examination of the gastric antrum of rat 01 in the blank group of Example 2 of the present invention at 400 times magnification;

[0013] Figure 2 The results of light microscopy examination of the gastric antrum of rat 06 in the blank group of Example 2 of the present invention at 400 times magnification;

[0014] Figure 3 The results of light microscopy examination of the gastric antrum of rat 07 in the blank group of Example 2 of the present invention at 400 times magnification;

[0015] Figure 4 The results of light microscopy examination of the gastric antrum of rat 19 in the model group of Example 2 of the present invention at 400 times magnification;

[0016] Figure 5 The results of light microscopy examination of the gastric antrum of rat 22 in the model group of Example 2 of the present invention at 400 times magnification;

[0017] Figure 6 The results of light microscopy examination of the gastric antrum of rat 23 in the model group of Example 2 of the present invention at 400 times magnification;

[0018] Figure 7 The results of light microscopy examination of the gastric antrum of 27 rats in the positive control group of Example 2 of the present invention at 400 times magnification;

[0019] Figure 8 The results of light microscopy examination of the gastric antrum of 28 rats in the positive control group of Example 2 of the present invention at 400 times magnification;

[0020] Fig. 9 The results of light microscopy examination of the gastric antrum of 33 rats in the positive control group of Example 2 of the present invention at 400 times magnification;

[0021] Fig.10 The results of light microscopic examination of the gastric antrum of 43 rats in the low-dose group of Example 2 of the present invention at 400 times magnification;

[0022] Fig.11 The results of light microscopic examination of the gastric antrum of 44 rats in the low-dose group of Example 2 of the present invention at 400 times magnification;

[0023] Fig.12 The results of light microscopy examination of the gastric antrum of 48 rats in the low-dose group of Example 2 of the present invention at 400 times magnification;

[0024] Fig.13 The results of light microscopy examination of the gastric antrum of 50 rats in the dosage group of Example 2 of the present invention at 400 times magnification;

[0025] Fig.14 The results of light microscopy examination of the gastric antrum of rats in the dosage group 52 in Example 2 of the present invention at 400 times magnification;

[0026] Fig.15 The results of light microscopy examination of the gastric antrum of rats in the dosage group 55 in Example 2 of the present invention at 400 times magnification;

[0027] Fig.16 The results of light microscopic examination of the gastric antrum of 68 rats in the high-dose group of Example 2 of the present invention at 400 times magnification;

[0028] Fig.17 The results of light microscopy examination of the gastric antrum of 69 rats in the high-dose group of Example 2 of the present invention at 400 times magnification;

[0029] Fig.18 The results of light microscopic examination of the gastric antrum of 72 rats in the high-dose group of Example 2 of the present invention at 400 times magnification are shown. DETAILED DESCRIPTION

[0030] The present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] a. After crushing the whole herb of Artemisia striata, add 10 mL of water to every 1 g of the crushed Artemisia striata and mix well to obtain a mixed solution;

[0033] b. Reflux the mixed solution obtained in step a for 3 times, each time for 1 hour, combine the reflux extracts and filter to obtain a filtrate;

[0034] c. recovering the filtrate obtained in step b under reduced pressure and concentrating it to obtain an Artemisia striata extract;

[0035] d. Disperse the Artemisia striata extract obtained in step c in water to obtain a suspension, extract it with n-butanol for 3 times, combine the extracts, concentrate under reduced pressure, and dry them in vacuum to obtain the n-butanol effective part extract of Artemisia striata.

[0036] Example 2

[0037] 1. Effect of the extract of the effective part of Artemisia ternata n-butanol on the functional dyspepsia rat model induced by the composite modeling method:

[0038] This experiment used irregular feeding + tail clamping stimulation + ice saline gavage to replicate the functional dyspepsia model;

[0039] After 7 days of adaptive feeding, SD rats were randomly divided into blank control group and model group according to body weight. During the modeling period, the blank control group was fed normally without modeling. The model group was fed on odd days and fasted on even days. The tail of the rat was clamped with a long sponge clamp at the end of one-third to make it scream and struggle, but not break it. It was stimulated and made to get angry and fight with other rats in the same cage (if any rat was scratched due to fighting, iodine was given to disinfect the wound to prevent infection), 30min / 2 times / d, and ice saline was given by gavage every day for 14 days. After the modeling, the rats in the model group were randomly divided into the model group, the positive control drug domperidone group 3.1mg / kg / d, and the low, medium and high dose groups of the effective part of Artemisia annua n-butanol extract 20.0, 50.0, 90.0mg / kg / d according to their body weight. The blank control group and the model group were then gavaged with distilled water, and the other positive drug control groups and the low, medium and high dose groups of the effective part of Artemisia annua n-butanol extract were gavaged with 10ml / kg for 14 days.

[0040] 1.1 General observations:

[0041] The changes in the fur, emotional state, behavioral state, excitement level, activity level, and feces of rats in each group were observed daily before and after modeling and drug administration;

[0042] 1.2 Changes in rat weight before and after the experiment:

[0043] The body weight of rats in each group was measured before the experiment (i.e., 1 day before the experiment), after the modeling, and after the administration, and the average body weight change of rats in each group was calculated.

[0044] 1.3 Determination of gastric juice volume:

[0045] The rats were fasted but not watered for 24 hours after the last administration. The rats were anesthetized with ether the next day. A small incision was made along the midline of the rat's abdomen, the stomach was gently found, the junction of the pylorus and the duodenum was ligated, and the abdominal wall incision was sutured. The rats were fasted and not watered for 2 hours after the operation. The rats were anesthetized again, the abdominal cavity was opened, the cardia was ligated, the whole stomach was removed, the blood was wiped with filter paper, the stomach cavity was cut open along the greater curvature, the stomach contents were poured out, the gastric juice was collected in a graduated centrifuge tube, and the gastric juice secretion was recorded; at the same time, the rats were centrifuged at 3500rpm / mi for 10min, and the supernatant was removed for gastric acid testing;

[0046] 1.4 Gastric acid determination:

[0047] Gastric acid was determined by acid-base titration. 1-2 drops of phenolphthalein indicator were added to 1 ml of gastric juice. The solution was titrated with 0.02 mol / L NaOH until the color turned red for 2 seconds. The amount of NaOH consumed was recorded. The calculation method was as follows:

[0048] Total acidity of gastric contents = (amount of NaOH consumed mL × 0.02 mol / L × 1000) / gastric juice secretion;

[0049] 1.5 Determine pepsin activity; ELISA method was used to determine motilin (MTL), gastrin (GAS) and 5-hydroxytryptamine (5-HT); the determination was carried out according to the instructions of the corresponding kits;

[0050] 1.6 Histopathological examination:

[0051] Part of the rat gastric antrum tissue was fixed with 10% formaldehyde, embedded in paraffin, sliced, and stained with HE for histopathological examination to observe the gastric tissue lesions;

[0052] 1.7 Data statistical processing method: All test data are based on The quantitative data were analyzed by one-way analysis of variance, and the count data were analyzed by χ2 test. P < 0.05 was considered statistically significant. The statistical software was SPSS version 25.0.

[0053] 1.8 Experimental results:

[0054] 1.8.1 General Behavioral Observations:

[0055] Before modeling, the rats in each group were in normal condition and activity; after modeling, the rats in the model group were irritable, often fighting each other, with common scratches on their limbs and heads, followed by reduced activity, listlessness, rough and dull hair, slow reaction, and loose feces. The rats in the blank control group were in normal condition and behavior, with shiny hair; after 14 days of drug intervention, the rats in each drug group showed different degrees of relief from the above conditions and gradually returned to normal;

[0056] 1.8.2 Weight changes:

[0057] During the modeling period, the weight gain rate of the blank group rats was significantly higher than that of the model group, and some model group rats showed negative weight gain, which was statistically different from the blank control group (P < 0.05). The weight gain of the model group and each drug-treated group gradually returned to normal after drug intervention. The low, medium and high dose groups of the effective part of Artemisia annua extract were statistically different from the model group (P < 0.05). The results are shown in Table 1.

[0058] Table 1 Effects of n-butanol effective fraction extraction from Artemisia argyi on body weight changes in FD rats

[0059]

[0060]

[0061] Note: vs blank group * P<0.05; vs model group # P < 0.05;

[0062] 1.8.3 Effects of the extract of n-butanol effective part of Artemisia ternata on gastric juice secretion, total acidity of gastric contents and pepsin activity in FD rats:

[0063] The experimental results showed that compared with the blank group, the gastric juice secretion of the model group rats was significantly reduced (P < 0.01), the total acidity of the gastric contents was significantly increased (P < 0.01), and the pepsin activity was significantly reduced (P < 0.01), with statistical differences; compared with the model group, the gastric juice secretion and pepsin activity of the positive drug group were significantly increased (P < 0.01), and the total acidity of the gastric contents was significantly reduced (P < 0.01); the low, medium and high dose groups of the effective part of Artemisia annua extract significantly increased the gastric juice secretion and pepsin activity, and reduced the total acidity of the gastric contents, among which the high dose and medium dose groups were more significant, with statistical differences (P < 0.01, P < 0.05); the results are shown in Table 2;

[0064] Table 2 Effects of the extract of n-butanol effective part of Artemisia ternata on gastric juice secretion, total acidity of gastric contents and pepsin activity in FD rats

[0065]

[0066] Note: vs blank group *p<0.05, **p<0.01; vs model group # p<0.05, ## p<0.01;

[0067] 1.8.4 Effects of the n-butanol extract of the effective part of Artemisia ternata on the levels of motilin (MTL), gastrin (GAS) and serotonin (5-HT) in the serum of FD rats:

[0068] The experimental results showed that compared with the blank group, the level of serum motilin (MTL) in the model group was significantly reduced (P < 0.01), and compared with the model group, the positive drug group could significantly increase the level of motilin (MTL) (P < 0.01), and the medium-dose group and high-dose group of the effective part extract of Artemisia striata n-butanol could also significantly increase the level of motilin (MTL) (P < 0.01).

[0069] Compared with the blank group, the model group had a significantly lower serum gastrin (GAS) level (P < 0.01), the positive drug group had a significantly higher serum gastrin (GAS) level (P < 0.01), and the medium-dose and high-dose groups of the n-butanol effective part extract of Artemisia striata could significantly increase the MTL level (P < 0.05, P < 0.01).

[0070] Compared with the blank group, the serum 5-HT level in the model group was significantly increased (P < 0.01), the positive drug group could significantly reduce the 5-HT level compared with the model group (P < 0.05), and the medium-dose group and high-dose group of the effective part of Artemisia annua extract of n-butanol could significantly reduce the 5-HT level (P < 0.05, P < 0.01). The detailed results are shown in Table 3.

[0071] Table 3 Effects of the n-butanol effective fraction extract of Artemisia argyi on the levels of MTL, GAS and 5-HT in the serum of FD rats

[0072]

[0073]

[0074] Note: vs blank group *p<0.05, **p<0.01; vs model group # p<0.05, ## p<0.01;

[0075] 1.8.5 Pathological tissue examination At the end of the experiment, the rat gastric antrum was dissected and routinely fixed, sliced ​​and stained. The results of light microscopy examination of some sections are shown in the figure. Figure 1-18 ;

[0076] Blank group: The rat gastric mucosa, submucosa, muscularis and adventitia were intact and clearly layered, and no obvious hyperplasia was observed in the muscularis and adventitia (10 / 10). Figure 1-3 ;

[0077] Model group: gastric glands in some areas of the gastric lamina propria of rats were neatly arranged, with clear tubular findings, capillary dilation in the superficial mucosa or submucosal layer, and no inflammatory cell infiltration was observed at the bottom of the gastric glands and in the submucosal layer (8 / 10). Figure 4-6 ;

[0078] Positive drug group: The gastric antrum tissue structure of rats was clear, the gastric mucosal epithelium was intact, the shape and structure of gastric glands were regular, and the mucosal muscular layer was regular (10 / 10). The results are shown in Figure 7-9 ;

[0079] Low-dose group: The gastric glands in the lamina propria of the rat stomach were arranged in a relatively neat pattern, and no inflammatory cell infiltration was observed at the bottom of the gastric glands or in the submucosal layer (9 / 10). Figure 10-12 ;

[0080] In the medium-dose group and the high-dose group, the gastric antrum tissue structure of the rats was clear, the gastric mucosal epithelium was intact, the shape and structure of the gastric glands were regular, and the mucosal muscular layer was regular (10 / 10). Figure 13-15 .

[0081] High-dose group: The gastric antrum tissue structure of rats was clear, the gastric mucosal epithelium was intact, the gastric glands had regular shape and structure, the mucosal muscular layer was regular, and no inflammatory cell infiltration was observed (10 / 10). Figure 16-18 .

[0082] Example 3

[0083] Pharmacodynamics experiment of the extract of the effective part of Artemisia argyi on gastrointestinal function in the functional dyspepsia mouse model:

[0084] The FD mouse model was established by irregular eating combined with intraperitoneal injection of L-arginine. The mice were randomly divided into a normal group and a modeling group according to their body weight. The mice in the modeling group were fed for 2 days and fasted for 1 day for a total of 10 days. On the 11th to 14th day, 0.1g / kg L-arginine was intraperitoneally injected; the mice in the normal group were routinely fed and intraperitoneally injected with an equal volume of normal saline; during the experiment, the daily status of the mice (body weight, water intake, food intake, hair glossiness and individual activity) was observed. After the modeling was completed, 5 mice were randomly selected from each group and killed, and the small intestinal propulsion rate and gastric emptying rate of the mice were measured. The results showed that the small intestinal propulsion rate and gastric emptying rate of the modeling group were lower than those of the normal group, indicating that the FD modeling was successful.

[0085] After successful modeling, the mice in the modeling group were randomly divided into the model group, the positive control drug domperidone group at 4.5 mg / kg, and the low, medium, and high dose groups of the effective part extract of Artemisia striata n-butanol at 50, 90, and 180 mg / kg, with an intragastric volume of 10 ml / kg / d. The normal group and the model group were intragastrically given the same volume of distilled water for 7 days, during which the weight changes of the mice were recorded.

[0086] On the 7th day, the mice were fasted but not watered for 24 hours after the last administration. Each mouse was gavaged with a semisolid paste (10 g of sodium carboxymethyl cellulose was dissolved in 250 ml of distilled water and stirred evenly, and 16 g of milk powder, 8 g of sugar, 8 g of starch and 2 g of activated carbon were added respectively and stirred evenly to prepare a black solid paste of 300 ml (about 300 g)) at a concentration of 0.8 ml / mouse. The mice were killed by cervical dislocation 20 minutes later, and the mice were quickly dissected. The gastric cardia and pylorus of the mice were ligated, and the complete stomach was cut off. The whole stomach was weighed after being wiped dry with filter paper. Then, the stomach body was cut open along the greater curvature of the stomach, and the stomach contents were washed out with saline and wiped dry. The net weight of the stomach was weighed. The difference between the whole stomach weight and the net stomach weight was taken as the weight of the residual content in the stomach, and the gastric emptying rate was calculated according to the formula. At the same time, the small intestine from the pylorus to the ileocecal region was quickly cut off, and the mesentery was gently peeled off and laid directly on white paper. The total length from the pylorus to the ileocecal region and the distance from the pylorus to the front of the semisolid paste were measured, and the small intestine propulsion rate was calculated according to the formula.

[0087] Gastric emptying rate % = [1-(total stomach weight-net stomach weight) / gastric weight] × 100%;

[0088] Small intestinal propulsion rate % = [distance from pylorus to the front of black semisolid paste / total length from pylorus to ileocecal part] × 100%;

[0089] Experimental results:

[0090] The experimental results showed that compared with the blank group, the gastric emptying rate and small intestinal propulsion rate of the model group were significantly reduced (P < 0.05); compared with the model group, the gastric emptying rate and small intestinal propulsion rate of the positive drug domperidone group were significantly increased (P < 0.05); the low, medium and high dose groups of the effective part of Artemisia striata also increased to varying degrees, among which the high dose group promoted gastric emptying rate and small intestinal propulsion rate more significantly (P < 0.01), with statistically significant differences, and the detailed results are shown in Table 4;

[0091] Table 4 Effects of the effective fraction of Artemisia argyi n-butanol on gastrointestinal function of FD model mice

[0092]

[0093] Note: VS blank * P<0.05, ** P<0.01; VS model group # P<0.05, ## P<0.01.

[0094] Example 4

[0095] Effects of the extract of n-butanol effective fraction of Artemisia ternata on gastrointestinal motility of normal mice:

[0096] ICR mice were randomly divided into a blank control group, an Artemisia striata n-butanol effective part extract group, and a positive drug metoclopramide group, with 8 mice in each group. The mice in the drug group were intragastrically administered with 90 mg / kg of Artemisia striata n-butanol effective part extract group and 4.5 mg / kg of positive drug metoclopramide group daily, and the mice in the blank group were intragastrically administered with an equal volume of distilled water for 7 days;

[0097] On the 6th day, the mice were fasted but not watered for 24 hours after oral administration. 30 minutes after the last administration, 2% dextran blue (BD-2000) solution was administered orally, 0.3 ml / mouse. 20 minutes later, the mice were killed by dislocation, the abdominal cavity was opened, the gastric cardia and pylorus of the mice were ligated, and the stomach was removed from the pyloric sphincter, cut open along the greater curvature of the stomach, and the residue in the stomach was fully dissolved with 2 ml of distilled water. The dissolved liquid was collected, centrifuged at 3500r / min for 15 minutes, and the supernatant was taken. The absorbance (OD) was measured at a wavelength of 620nm with distilled water, and the average absorbance of the blank control group was taken as 100%, and then the average of each group was calculated; the relative gastric pigment residue rate was calculated, which was used as an indicator of gastric emptying function.

[0098] At the same time, quickly cut off the small intestine from the pylorus to the ileocecal part, gently peel off the mesentery, and lay it straight on white paper. Measure the distance from the pyloric sphincter to the anterior pigment segment and from the pylorus to the cecum, and calculate the small intestine propulsion rate according to the formula;

[0099] Pigment residual rate in the stomach % = absorbance value of stomach contents / mean absorbance value of blank control group × 100%;

[0100] Small intestinal propulsion rate % = small intestinal propulsion length / small intestinal length × 100%;

[0101] The experimental results showed that compared with the blank group, the n-butanol fraction of Artemisia striata could significantly increase the small intestinal propulsion rate (P < 0.01), significantly reduce the relative residual rate in the stomach, and significantly increase the gastric emptying rate (P < 0.01). The detailed results are shown in Table 5.

[0102] Table 5 Effects of the effective fraction of Artemisia annua n-butanol on gastrointestinal function of normal mice

[0103]

[0104] Note: VS blank group * P<0.05, ** P<0.01.

[0105] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

Claims

1. A method for preparing an extract of n-butanol effective part of Artemisia striata, Features Follow these steps: a. After crushing the whole herb of Artemisia striata, add 10 mL of water to every 1 g of the crushed Artemisia striata and mix well to obtain a mixed solution; b. Reflux the mixed solution obtained in step a for 3 times / 1h, combine the reflux extracts and filter to obtain a filtrate; c. recovering the filtrate obtained in step b under reduced pressure and concentrating it to obtain an Artemisia striata aqueous extract; d. Disperse the Artemisia striata aqueous extract obtained in step c in water to obtain a suspension, extract with n-butanol three times, combine the extracts, concentrate under reduced pressure, and vacuum dry to obtain the n-butanol effective part extract of Artemisia striata.

2. Use of the n-butanol effective fraction extract of Artemisia limbi obtained by the method as claimed in claim 1 in the preparation of a medicament for treating functional dyspepsia.

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