Neutralizing monoclonal antibodies against pseudorabies virus gD protein, hybridoma cells and their applications

By constructing and purifying neutralizing monoclonal antibodies and hybridoma cells against pseudorabies virus gD protein, the problem of prevention and control of pseudorabies virus infection was solved, efficient virus neutralization and vaccine development were achieved, and support for the prevention, control and treatment of pseudorabies was provided.

CN119192352BActive Publication Date: 2025-09-23ZHENGZHOU UNIV +1
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Patent Information

Application Number
CN202411250898.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-06
Publication Date
2025-09-23
Estimated Expiration
2044-09-06

AI Technical Summary

Technical Problem

Existing technologies lack effective neutralizing monoclonal antibodies against pseudorabies virus (PRV), which makes the prevention, control and treatment of pseudorabies difficult. PRV infection has a high mortality rate and is highly contagious, affecting the animal husbandry industry and human health.

Method used

Develop neutralizing monoclonal antibodies and hybridoma cells against pseudorabies virus gD protein. By constructing a PRV gD eukaryotic expression vector, transducing it into CHO cells for expression, and purifying it using Ni column affinity chromatography, neutralizing monoclonal antibodies are prepared. Combined with a live attenuated vaccine for animal immunization, highly effective neutralizing monoclonal antibodies are screened and identified.

Benefits of technology

The prepared neutralizing monoclonal antibodies have high titer and specificity, can effectively neutralize PRV, are used in vaccine development and virus detection, provide support for the prevention, control and treatment of pseudorabies, and have good protection and treatment capabilities.

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Abstract

This application relates to a neutralizing monoclonal antibody, hybridoma cell, and application thereof against pseudorabies virus gD protein. This application constructs a PRV gD eukaryotic expression vector, transduces it into CHO cells for expression, and then immunizes animals with PRV gD recombinant protein and a live attenuated vaccine as immunogens to produce a neutralizing monoclonal antibody against PRV gD. The neutralizing monoclonal antibody or hybridoma cell provided herein lays the foundation for the development of rapid detection technologies for pseudorabies virus and its gD protein. These antibodies have broad research and application value and commercial utility in pseudorabies virus-related immunoassays, and provide important guidance for the development of pseudorabies virus-based vaccines.
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Citation Information

Patent Citations

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  • PRV (pesudorabies virus) gB monoclonal antibody and application thereof

    CN107459574A