Composition with brain strengthening and vision benefiting effects and application thereof

Abstract

The invention relates to a composition with brain strengthening and vision benefiting effects and application of the composition. The composition is prepared from the following components in parts by mass: 1 to 15 parts of DHA algae oil powder, 1 to 15 parts of walnut peptide, 0.1 to 0.5 part of lutein ester, 0.1 to 2 parts of tea leaf theanine, 4 to 30 parts of fructus lycii instant powder and 4 to 20 parts of blueberry fruit powder. The composition can be prepared into liquid beverage products, and can be brewed into cereal products, granules, capsules, tablets, powder, paste, pills, oral liquid and the like. The composition has the effects of strengthening the brain and benefiting vision, can effectively improve memory and visual fatigue symptoms and improve photopic vision persistence, and is simple, scientific, safe and reliable in product formula.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and in particular to a composition having brain-boosting and vision-enhancing effects, and a preparation method and application thereof. Background Art

[0002] In modern work and life, both students and young and middle-aged intellectual workers are increasingly using electronic products. Coupled with the high pressure of study and life, the market demand for eye care and brain care has increased. At present, there are many products on the market that claim to have the effect of assisting in improving memory and relieving visual fatigue. Products that improve memory are mainly composed of DHA, EPA, vitamins, zinc and other ingredients. Traditional Chinese medicine products often contain Alpinia oxyphylla, walnuts, Gastrodia elata, etc. Common ingredients in related studies on relieving visual fatigue include lutein esters, zeaxanthin, carotene, blueberry extract, etc. Traditional Chinese medicine products often contain wolfberry, chrysanthemum, cassia seed, blueberry, etc.

[0003] DHA algae oil is produced from the species of Schizochytrium (or Uken's chytrid or Crypthecodinium cohnii) through fermentation, separation, purification and other processes. It was approved as a new food raw material in 2010. Lutein ester is produced from marigold flowers through dehydration and crushing, solvent extraction, low molecular weight alcohol purification and vacuum concentration, and is approved as a new food raw material. Tea theanine is made from tea leaves through extraction, filtration, concentration and other processes, and is also approved as a new food raw material. Walnut peptide can be produced from walnut protein through enzymatic hydrolysis, separation, filtration, spray drying and other processes. Walnut protein hydrolysate can significantly improve the behavior of mice with cognitive and memory disorders, and restore the cholinergic system disorder and oxidative stress level in the mouse brain to normal. The high content of arginine, aspartic acid and glutamic acid in walnuts can enhance the memory improvement effect of walnut peptides at a certain dose, and has the effects of tonifying the kidney, warming the lungs and moisturizing the intestines. Lycium barbarum contains active ingredients such as polysaccharides, betaine and carotenoids, which have the effects of nourishing the liver and kidneys, improving sperm quality and improving eyesight. At present, research has found that the main components of wolfberry that improve visual fatigue are carotenoids and polysaccharides. Lutein and zeaxanthin are the main components of wolfberry carotenoids, which have potential effects in the treatment and prevention of eye diseases such as age-related macular degeneration and retinitis pigmentosa. Studies have shown that zeaxanthin can improve the vision of patients with retinopathy, protect the oxidative damage of the eye lens, reduce the turbidity of the lens, and delay the course of cataracts. Lycium barbarum polysaccharides can alleviate the oxidative stress response of the retina caused by different reasons, protect retinal ganglion cells, increase cell survival rate and reduce apoptosis. Blueberries contain anthocyanins, anthocyanins, flavonoids and their glycoside derivatives, phenolic acids and sugars. Blueberry anthocyanins have strong antioxidant effects, can promote collagen synthesis in the body, strengthen the metabolism of epidermal cells, and can also inhibit the formation of myopia in experimental animals by inhibiting the expression of MMP-2 protein, reducing collagen degradation.

[0004] Most of the products currently seen on the market are single products for improving memory or relieving visual fatigue, and products with brain-boosting and vision-benefiting effects are rare. For example, a brain-boosting and intelligence-enhancing composition disclosed in Chinese patent CN108651996A and its application are composed of phosphatidylserine, phospholipids, walnut peptides, DHA algae oil powder, L-theanine, senna acid, Poria extract, dried orange peel extract, intelligence-enhancing extract, Perilla extract, and saffron extract. In addition to using walnut peptides, DHA algae oil powder, and theanine, it must also rely on adding Poria extract, intelligence-enhancing extract, Perilla extract, and saffron extract to achieve technical effects, and its composition is complex. Although CN102210474A discloses a kind of eye-protecting and brain-boosting effervescent tablet, it is composed of active ingredients lutein ester, taurine, DHA, and acid-base auxiliary materials. The taurine component is indispensable. However, if taurine is consumed in excess, it can cause arrhythmia, and it is used in food formulations and must meet regulatory limit requirements. The use of taurine as a food ingredient should comply with the provisions of GB14880 "Standard for the Use of Food Nutrition Fortifiers". This standard clearly states that 13 varieties of taurine are allowed to be used, including modulated milk powder, jelly, etc. The usage limit for each variety is different. For example, the usage in flavored beverages should be controlled at 0.4g / kg~0.6g / kg. Therefore, although taurine has a positive effect on eye health such as visual fatigue, there are many restrictions in its later application. Summary of the invention

[0005] The purpose of the present invention is to solve the deficiencies of the prior art and provide a composition that has both brain-boosting and vision-enhancing effects, so as to improve the memory and visual fatigue symptoms of the applicable population and increase the duration of clear vision. The present invention also provides a preparation method and application of the composition.

[0006] The technical solution adopted by the present invention is as follows: A composition with both brain-boosting and vision-enhancing functions, comprising the following components in parts by mass: 1-15 parts of DHA algae oil powder, 1-15 parts of walnut peptides, 0.1-0.5 parts of lutein esters, 0.1-2 parts of tea theanine, 4-30 parts of wolfberry instant powder, and 4-20 parts of blueberry fruit powder.

[0007] Preferably, the composition of the present invention is composed of the following ingredients in parts by mass: 1-5 parts of DHA algae oil powder, 1-5 parts of walnut peptides, 0.1-0.2 parts of lutein esters, 0.1-0.5 parts of tea theanine, 5-10 parts of wolfberry instant powder, and 4-8 parts of blueberry powder.

[0008] More preferably, the composition of the present invention is composed of the following ingredients in parts by mass: 1 part of DHA algae oil powder, 1 part of walnut peptide, 0.2 parts of lutein ester, 0.3 parts of tea theanine, 8 parts of wolfberry instant powder, and 4 parts of blueberry fruit powder.

[0009] Furthermore, the preparation method of the wolfberry instant powder is to use wolfberry as a raw material, soak it in 5-20 times the amount of water for 2-5 hours to prepare a pulp that can pass through a 20-mesh sieve, add 1%-8% of the weight of the wolfberry enzyme, stir evenly and heat the pulp to 40-60°C for 1-2 hours, heat the pulp to boiling, centrifuge and filter, take the filtrate and concentrate it at 80°C under reduced pressure, add 30%-45% of the weight of the concentrate maltodextrin to the concentrate, mix well, and spray dry to obtain the powder; the enzyme is cellulase: pectinase prepared in a mass ratio of (4-6): (1-2). The preparation method uses a relatively low temperature to add the enzyme for extraction, thereby increasing the extraction rate while reducing the damage to the active ingredients.

[0010] Furthermore, the polysaccharide content in the wolfberry instant powder is ≥2wt.%.

[0011] Furthermore, the blueberry powder preparation method is to use fresh blueberries as raw materials, prepare fruit pulp that can pass through a 20-mesh sieve, add 0.2%-5% enzyme by weight of fresh blueberries, stir evenly and heat the fruit pulp to 40-60°C for 1-2 hours, heat the fruit pulp to boiling, centrifuge and filter, take the filtrate and concentrate it under reduced pressure at 80°C, add 30%-45% maltodextrin by weight of the concentrate to the concentrate and spray dry to obtain the powder; the cellulase:pectinase is prepared in a mass ratio of (1-2): (4-6). The preparation method uses a relatively low temperature to add enzymes for extraction, thereby increasing the extraction rate while reducing the damage to the active ingredients.

[0012] Further, the preparation process of the DHA algae oil powder is based on DHA algae oil with a DHA content of at least 50% as a basic raw material, by mass ratio, whey protein powder of 80%-85% of DHA algae oil, maltodextrin of 30%-36%, sodium caseinate of 5%-7%, citric acid fatty acid glyceride of 6%-8%, sodium ascorbate of 0.1%-1%, whey protein powder, maltodextrin, sodium caseinate, citric acid fatty acid glyceride and sodium ascorbate are dissolved in twice the amount of warm water of 55°C-65°C, emulsified at 10000rpm-15000rpm for 10min-15min, then DHA algae oil is added, emulsified at 10000rpm-15000rpm for 15min-30min, homogenized at 50Mpa-60Mpa for 2-3 times, and spray dried. The DHA algae oil powder obtained by this preparation process has better stability, water solubility and fluidity, and is convenient for product processing.

[0013] The application of the composition having both brain-boosting and vision-benefiting effects of the present invention is to dissolve the DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder, and blueberry fruit powder in pure water, mix them evenly, filter them, and pack them to obtain a liquid beverage product having both brain-boosting and vision-benefiting effects. Alternatively, the DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder, and blueberry fruit powder are mixed, and then cereal powder is added, mixed evenly, and then packed to obtain a reconstituted cereal product having both brain-boosting and vision-benefiting effects. Alternatively, the DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder, and blueberry fruit powder are mixed, and after adding suitable additives, they are made into one of granules, capsules, tablets, powders, pastes, pills, and oral liquids.

[0014] In the composition of the present invention, DHA algae oil powder has the effect of assisting in improving memory, walnut peptide has the ability to scavenge hydroxyl radicals and good effect of improving memory activity, lutein ester has anti-oxidation, increases the effect of lutein level in the retinal macula, tea theanine has the effects of improving sleep, anti-depression, improving cognition and protecting nerves, wolfberry instant powder has the effects of nourishing the liver and kidney, improving the essence and improving eyesight, and blueberry fruit powder has the effects of anti-oxidation, relieving human visual fatigue, etc. After these components are mixed, they will not produce effects to offset each other, and the compatibility of walnut peptide, lutein ester and blueberry fruit powder ensures the antioxidant effect of the composition, and reduces the oxidative stress damage of eye cells. When tea theanine is used to improve sleep, it is coordinated with DHA algae oil powder and walnut peptide to improve memory and promote brain health care.

[0015] Compared with the prior art, the advantages of the technology of the present invention are as follows: 1. The raw materials used in the composition of the present invention are all food raw materials, which are highly safe to eat and have little side effects when used for a long time. The proportion and dosage of the food raw materials meet the requirements of relevant regulations. The processing methods of wolfberry instant powder and blueberry fruit powder are unique, and no organic solvents are used, which are highly safe and will not leave solvent odor.

[0016] 2. The present invention does not use excessive use to produce adverse effects and uses taurine that is strictly controlled as a raw material, which is safer and more reliable. At the same time, the present invention directly uses algae oil powder with a high DHA content as a raw material, and the content of the effective ingredient is clearer. The DHA content of DHA algae oil powder is relatively high, reaching more than 35g / 100g. Compared with the fish oil with a DHA content of 36-125mg / g used in the prior art products, the DHA content of DHA algae oil powder under the same dosage is equivalent to 2.8-9.7 times that of fish oil, and the effect is better.

[0017] 3. The present invention has obtained a new composition that has both brain-boosting and vision-enhancing effects through in-depth research. The composition has a simple formula and avoids interference and influence between multiple components compared to traditional Chinese medicine compound prescriptions. The present invention embodiment has conducted a food tasting experiment with reference to the health food function evaluation method, and the results show that it has good brain-boosting and vision-enhancing effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is the latency period of the light-dark box animal model experiment and the number of errors in entering the dark room within 5 minutes.

[0019] Figure 2 This is the number of animals and time results that reached the endpoint within 2 minutes in the water maze animal model experiment. DETAILED DESCRIPTION

[0020] The present invention is further described below in conjunction with the embodiments. The embodiments are only partial embodiments of the present invention and are not intended to limit the scope of protection of the present invention. All embodiments listed within the scope of the claims of the present invention belong to the scope of protection of the present invention. Example 1

[0021] The composition with both brain-boosting and vision-enhancing effects is composed of 1 part of DHA algae oil powder, 1 part of walnut peptide, 0.1 part of lutein ester, 0.1 part of tea theanine, 4 parts of wolfberry instant powder, and 4 parts of blueberry powder. The wolfberry instant powder is made of wolfberry, which is soaked in 5 times the amount of water for 2 hours to make a pulp that can pass through a 20-mesh sieve, and 5% of the weight of the wolfberry enzyme is added, and the enzyme ratio is cellulase: pectinase = 4:1. Stir evenly and heat the pulp to 45°C for 2 hours. After heating the pulp to boiling, centrifuge and filter, take the filtrate and concentrate it under reduced pressure at 80°C, add 30% maltodextrin to the concentrate, mix well, and spray dry to obtain the product. Blueberry fruit powder is made from fresh blueberries, made into a pulp that can pass through a 20-mesh sieve, and 0.2% enzyme is added, the enzyme ratio is cellulase: pectinase = 1:4, stirred evenly and the pulp is heated to 40°C for 2 hours, the pulp is heated to boiling and then centrifuged and filtered, the filtrate is concentrated at 80°C under reduced pressure, 30 maltodextrin is added to the concentrate and mixed well, and spray dried. The preparation process of DHA algae oil powder is based on DHA algae oil (DHA content 50%) as the basic raw material, 85% whey protein powder, 30% maltodextrin, 7% sodium caseinate, 6% citric acid fatty acid glyceride, 0.2% sodium ascorbate are taken, dissolved in twice the amount of warm water at 58°C, emulsified at 13000rpm for 10min, DHA algae oil is added, emulsified at 13000rpm for 30min, homogenized twice at 60Mpa, and then spray dried.

[0022] Mix all the raw materials of the composition, including DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder and blueberry fruit powder, evenly and dissolve them in 5 times the amount of pure water, add appropriate amount of sweetener, and add edible flavors of different flavors according to taste requirements. After mixing evenly, filter, package and sterilize with wet heat to obtain a liquid beverage product with brain-enhancing and vision-enhancing effects. Example 2

[0023] The composition with both brain-boosting and vision-enhancing effects is composed of 15 parts of DHA algae oil powder, 15 parts of walnut peptides, 0.5 parts of lutein esters, 2 parts of tea theanine, 30 parts of wolfberry instant powder, and 20 parts of blueberry powder. The wolfberry instant powder is made of wolfberry fruit as raw material, and is soaked in 20 times the amount of water for 5 hours to make a pulp that can pass through a 20-mesh sieve, and 8% of the weight of the wolfberry is added. The enzyme ratio is cellulase: pectinase = 3:1, and the mixture is stirred evenly and heated to 60°C for 1 hour. After the pulp is heated to boiling, it is centrifuged and filtered, and the filtrate is concentrated under reduced pressure at 80°C. 45% maltodextrin is added to the concentrate and spray-dried according to a conventional method. Blueberry powder is made from fresh blueberries, and is made into a pulp that can pass through a 20-mesh sieve. 5% enzymes are added, and the ratio of enzymes is cellulose: pectinase = 1:3. Stir evenly and heat the pulp to 60°C for 1 hour. After the pulp is heated to boiling, it is centrifuged and filtered. The filtrate is concentrated under reduced pressure at 80°C, and 45% maltodextrin is added to the concentrate and spray-dried according to the conventional method. The preparation process of DHA algae oil powder is based on DHA algae oil (DHA content 50%). 83% whey protein powder, 30% maltodextrin, 7% sodium caseinate, 6% citric acid fatty acid glyceride, and 0.5% sodium ascorbate are taken from the mass of DHA algae oil powder. Dissolve it in twice the amount of warm water at 65°C, emulsify at 14000rpm for 15min, add DHA algae oil, emulsify at 14000rpm for 25min, homogenize twice at 55Mpa, and spray-dry according to the conventional method.

[0024] Mix all the raw materials of the composition evenly, add cereal powder raw materials such as cooked brown rice powder, cooked oatmeal powder, add appropriate amount of seasoning additives or ingredients, and add edible flavors of different flavors according to taste requirements, mix evenly and then package to obtain the instant cereal product with brain-boosting and vision-enhancing effects. Example 3

[0025] The composition with both brain-boosting and vision-enhancing effects is composed of 1 part of DHA algae oil powder, 1 part of walnut peptide, 0.2 parts of lutein ester, 0.3 parts of tea theanine, 8 parts of wolfberry instant powder, and 4 parts of blueberry powder. The wolfberry instant powder is made of wolfberry fruit as raw material, and is soaked in 12 times the amount of water for 3 hours to make a pulp that can pass through a 20-mesh sieve, and 6% of the weight of the wolfberry is added. The enzyme ratio is cellulase: pectinase = 2:1, and the mixture is stirred evenly and heated to 50°C for 1.5 hours. After the pulp is heated to boiling, it is centrifuged and filtered, and the filtrate is concentrated under reduced pressure at 80°C. 35% maltodextrin is added to the concentrate and spray-dried according to a conventional method. Blueberry fruit powder is made from fresh blueberries, made into a pulp that can pass through a 20-mesh sieve, and 0.8% enzyme is added, the enzyme ratio is cellulase: pectinase = 1:2, stirred evenly and the pulp is heated to 50°C for 1.5 hours, the pulp is heated to boiling and then centrifuged and filtered, the filtrate is concentrated under reduced pressure at 80°C, 35% maltodextrin is added to the concentrate and spray-dried according to the conventional method. The preparation process of DHA algae oil powder is based on DHA algae oil (DHA content 50%) as the basic raw material, 80% whey protein powder, 35% maltodextrin, 6% sodium caseinate, 8% citric acid fatty acid glyceride, 0.3% sodium ascorbate are taken, dissolved in twice the amount of warm water at 55°C, emulsified at 10000rpm for 10min, DHA algae oil is added, emulsified at 15000rpm for 30min, homogenized at 55Mpa for 3 times, and spray-dried according to the conventional method.

[0026] Mix all the raw materials of the composition evenly, add 5% of the total weight of erythritol (60-80 mesh powder) and 0.15% of citric acid, or add other food ingredients or food additives according to taste requirements, further mix evenly, and then package into bags of 10g of solid beverage to obtain a solid beverage product with brain-boosting and vision-enhancing effects. Example 4

[0027] The composition with both brain-boosting and vision-enhancing effects is composed of 3 parts of DHA algae oil powder, 1 part of walnut peptide, 0.15 parts of lutein ester, 0.3 parts of tea theanine, 7 parts of wolfberry instant powder, and 6 parts of blueberry powder. The wolfberry instant powder is made of wolfberry fruit as raw material, and is soaked in 12 times the amount of water for 3 hours to make a pulp that can pass through a 20-mesh sieve, and 5% of the weight of the wolfberry is added. The enzyme ratio is cellulase: pectinase = 5:2, and the mixture is stirred evenly and heated to 55°C for 2 hours. After the pulp is heated to boiling, it is centrifuged and filtered, and the filtrate is concentrated under reduced pressure at 80°C. 40% maltodextrin is added to the concentrate and spray-dried according to the conventional method. Blueberry powder is made from fresh blueberries, and is made into a pulp that can pass through a 20-mesh sieve. 2.5% enzymes are added, and the ratio of enzymes is cellulase: pectinase = 2:5. Stir evenly and heat the pulp to 55°C for 2 hours. After the pulp is heated to boiling, it is centrifuged and filtered. The filtrate is concentrated under reduced pressure at 80°C, and 40% maltodextrin is added to the concentrate and spray-dried according to the conventional method. The preparation process of DHA algae oil powder is based on DHA algae oil (DHA content 50%). 85% whey protein powder, 36% maltodextrin, 7% sodium caseinate, 6% citric acid fatty acid glyceride, and 0.8% sodium ascorbate are taken as the basic raw material. Dissolve it in twice the amount of warm water at 60°C, emulsify at 10000rpm for 15min, add DHA algae oil, emulsify at 15000rpm for 20min, homogenize 3 times at 50Mpa, and spray-dry it according to the conventional method.

[0028] Mix all the raw materials of the composition evenly, add 8 times the amount of sorbitol, 3 times the amount of xylitol, and 0.2 times the amount of magnesium stearate and mix evenly, and solid edible flavors of different flavors can also be added according to taste requirements. All the raw materials are mixed evenly, and directly pressed into tablets according to conventional methods to obtain a compressed candy product with brain-enhancing and vision-enhancing effects. Example 5

[0029] The composition with both brain-boosting and vision-enhancing effects is composed of 1 part of DHA algae oil powder, 1 part of walnut peptide, 0.1 part of lutein ester, 0.1 part of tea theanine, 5 parts of wolfberry instant powder, and 4 parts of blueberry powder. The wolfberry instant powder is made of wolfberry, which is soaked in 15 times the amount of water for 2 hours to make a pulp that can pass through a 20-mesh sieve, and 1%-8% of the weight of the wolfberry is added. The enzyme ratio is cellulase: pectinase = 5:2, and the mixture is stirred evenly and heated to 50°C for 1 hour. After the pulp is heated to boiling, it is centrifuged and filtered, and the filtrate is concentrated under reduced pressure at 80°C. 35% maltodextrin is added to the concentrate and spray-dried according to a conventional method. Blueberry powder is made from fresh blueberries, and is made into a pulp that can pass through a 20-mesh sieve. 1.5% enzyme is added, and the ratio of enzymes is cellulase: pectinase = 1:5. Stir evenly and heat the pulp to 50°C for 1-2 hours. After the pulp is heated to boiling, it is centrifuged and filtered. The filtrate is concentrated under reduced pressure at 80°C, and 32% maltodextrin is added to the concentrate and spray-dried according to the conventional method. The preparation process of DHA algae oil powder is based on DHA algae oil (DHA content 50%). 85% whey protein powder, 30% maltodextrin, 5% sodium caseinate, 8% citric acid fatty acid glyceride, and 0.6% sodium ascorbate are taken from the mass of DHA algae oil powder. Dissolve it in twice the amount of warm water at 58°C, emulsify at 12000rpm for 15min, add DHA algae oil, emulsify at 13000rpm for 25min, homogenize 3 times at 50Mpa, and spray-dry according to the conventional method.

[0030] Mix all the raw materials of the composition evenly, dissolve them with 3-5 times the amount of water, add 5% fructose syrup, 0.1% citric acid and 0.1% pectin to dissolve, stir and mix well, and then fill into 30ml / bottle of oral liquid product. Example 6

[0031] The composition with both brain-boosting and vision-enhancing effects is composed of 5 parts of DHA algae oil powder, 5 parts of walnut peptide, 0.2 parts of lutein ester, 0.5 parts of tea theanine, 10 parts of wolfberry instant powder, and 8 parts of blueberry powder. The wolfberry instant powder is made of wolfberry fruit as raw material, and is soaked in 10 times the amount of water for 3 hours to make a pulp that can pass through a 20-mesh sieve, and 6% of the weight of wolfberry is added. The enzyme ratio is cellulase: pectinase = 5:2, and the mixture is stirred evenly and heated to 50°C for 2 hours. After the pulp is heated to boiling, it is centrifuged and filtered, and the filtrate is concentrated under reduced pressure at 80°C. 35% maltodextrin is added to the concentrate and spray-dried according to a conventional method. Blueberry fruit powder is made from fresh blueberries, made into a pulp that can pass through a 20-mesh sieve, and 3.6% enzymes are added, the enzyme ratio is cellulase: pectinase = 1.6:5.5, stirred evenly and the pulp is heated to 55°C for 1.5 hours, the pulp is heated to boiling and then centrifuged and filtered, the filtrate is concentrated at 80°C under reduced pressure, 30% maltodextrin is added to the concentrate and spray-dried according to the conventional method. The preparation process of DHA algae oil powder is based on DHA algae oil (DHA content 50%) as the basic raw material, 80% whey protein powder, 30% maltodextrin, 7% sodium caseinate, 6% citric acid fatty acid glyceride, 0.2% sodium ascorbate are taken, dissolved in twice the amount of warm water at 50°C, emulsified at 10000rpm for 15min, DHA algae oil is added, emulsified at 15000rpm for 15min, homogenized 3 times at 60Mpa, and spray-dried according to the conventional method.

[0032] All the raw materials of the composition are mixed evenly, and appropriate amounts of microcrystalline cellulose or modified starch, silicon dioxide, and magnesium stearate are added according to the preparation requirements, mixed evenly, and then filled into hard capsule shells to obtain a capsule product with brain-boosting and vision-enhancing effects.

[0033] The composition of the embodiment of the present invention was used as the research object to conduct animal model experiments and human food trial experiments.

[0034] Animal model test materials and methods: The light and dark box test and water maze test of mice were carried out in accordance with the test method of assisting in improving memory function in the functional test and evaluation method of health food. SPF female Kunming mice were used as test animals. Three dose groups and a blank control group were set up. The gavage doses were 1.2, 2.4, and 3.6 g / kg BW, respectively. The blank control group was treated with distilled water by gavage. There were 10 test animals in each dose group. The test animals were gavaged continuously for 30 days, and the test began 24 hours after the last gavage. The experimental method was tested and inspected in accordance with the "Health Food Function Test and Evaluation Method".

[0035] Light-dark box test: Training began the next day after the last sample administration. During the experiment, the mouse was placed in the light chamber with its face facing away from the hole, and a timer was started at the same time. The animal passed through the hole and entered the dark chamber and was shocked. The timer stopped automatically, and the mouse was taken out. The time required for each mouse to enter the light chamber and receive the shock was recorded. This was the latency period. The training lasted for 5 minutes, and the number of shocks within 5 minutes was recorded. The test was repeated 24 hours later, and the latency period for each animal to enter the dark chamber and the number of shocks within 5 minutes were recorded. The results showed that the body weight of mice in each dose group did not change significantly compared with the blank control group, and the difference was not statistically significant (P>0.05). The latency period of the medium and high dose groups was significantly prolonged, and the number of errors in entering the dark chamber within 5 minutes in the high dose group was significantly reduced. Compared with the blank control group, the difference was significant (P<0.05). The results of the light-dark box experiment were positive. The average values ​​of the results of Examples 1-6 were compared with the control group and the model group. Figure 1 .

[0036] Water maze test: Training began the day after the last sample was given. Samples were given once a day during the training period. The water depth of the maze lane was 9 cm, and the water temperature was about 20°C (≮15°C). The training time for mice was limited to 2 minutes, and mice that did not reach the end point within 2 minutes were counted as 2 minutes. Before the first training, the mice were placed near the ladder and allowed to climb up automatically 3 times. Before each subsequent training, the mice were placed near the ladder with their backs facing the stairs and allowed to climb up automatically once. The experiment was carried out in stages, and the distance was gradually lengthened depending on the learning performance of the animals. During the first training, a baffle was used to block the test point and training began, and the time from the test point to the end point was recorded. The second training extended the distance, starting from the second test point, and this distance was trained about 3 times. After more than 80% of the animals reached the end point within 2 minutes, the distance was extended again, and the time required for each mouse to reach the end point from the second test point and the number of errors occurred were recorded respectively (entering any blind end once was counted as an error). The last test was carried out from the starting point, and the mice were placed at the starting point, and the time required to reach the end point from the starting point and the number of errors occurred were recorded. The results showed that the weight of mice in each dose group did not change significantly compared with the blank control group, and the difference was not statistically significant (P>0.05); the number of animals reaching the end point within 2 minutes in each dose group increased significantly, and the difference between the medium and high dose groups and the blank control group was significant (P<0.05); the time for mice in the medium and high dose groups to reach the end point was lower than that in the blank control group, and the high dose group was significant (P<0.05). The water maze test results were positive, and the average values ​​of the results of Examples 1-6 were compared with the control group and the model group. Figure 2 .

[0037] Human food tasting experiment: Volunteers were recruited from students and office workers aged 18-35 years old who had no basic eye diseases and used their eyes for a long time. 100 volunteers were randomly divided into two groups, namely the control group and the test group. The test group consumed 10g / day of this product for 60 consecutive days. Visual acuity was checked on the 0th and 60th days of the tasting and surveyed through questionnaires. The symptom improvement efficiency was determined to be the improvement of 3 out of 8 symptoms such as eye pain, eye swelling, blurred vision, and dry eyes, and the other symptoms did not worsen, which was considered to be the improvement of visual fatigue symptoms.

[0038] Table 1 Symptom assessment scoring method

[0039] Visual fatigue can be evaluated by testing the persistence of clear vision. When the excitability of the human cerebral cortex decreases, the visual analysis function decreases. When the eyes look at the object, the time of not being able to see clearly increases, and the time of being able to see clearly decreases. This percentage of clear vision time to gaze time is called the persistence of clear vision. The persistence of clear vision is measured in accordance with the "Health Food Function Test and Evaluation Method". The experimental results are shown in Table 2.

[0040] Table 2 Results of human food tasting experiment

[0041] The above animal model tests and human food testing experiments prove that the composition of the present invention has the effects of strengthening the brain and improving vision, and can specifically improve memory and visual fatigue symptoms, and increase the duration of clear vision.

[0042] The raw materials used in the present invention, such as DHA algae oil, walnut peptide, lutein ester, tea theanine, wolfberry, blueberry, etc., can all be purchased commercially.

[0043] Unless otherwise specified, all percentages described in the present invention are by mass percentages.

Claims

1. A composition having both brain-boosting and vision-enhancing effects, characterized in that: The composition consists of the following ingredients in parts by mass: 1-15 parts of DHA algae oil powder, 1-15 parts of walnut peptides, 0.1-0.5 parts of lutein ester, 0.1-2 parts of tea theanine, 4-30 parts of wolfberry instant powder, and 4-20 parts of blueberry fruit powder.

2. A composition having both brain-boosting and vision-enhancing effects as claimed in claim 1, characterized in that: The composition consists of the following ingredients in parts by mass: 1-5 parts of DHA algae oil powder, 1-5 parts of walnut peptides, 0.1-0.2 parts of lutein ester, 0.1-0.5 parts of tea theanine, 5-10 parts of wolfberry instant powder, and 4-8 parts of blueberry fruit powder.

3. A composition having both brain-boosting and vision-enhancing effects as claimed in claim 2, characterized in that: The composition consists of the following ingredients in parts by mass: 1 part of DHA algae oil powder, 1 part of walnut peptide, 0.2 parts of lutein ester, 0.3 parts of tea theanine, 8 parts of wolfberry instant powder, and 4 parts of blueberry fruit powder.

4. A composition having both brain-boosting and vision-enhancing effects as claimed in claim 1, 2 or 3, characterized in that: The preparation method of the instant wolfberry powder is to use wolfberry as a raw material, soak it in 5-20 times the amount of water for 2-5 hours to make a pulp that can pass through a 20-mesh sieve, add 1%-8% of the weight of the wolfberry enzyme, stir evenly and heat the pulp to 40-60°C for 1-2 hours, heat the pulp to boiling, centrifuge and filter, take the filtrate and concentrate it at 80°C under reduced pressure, add 30%-45% of the weight of the concentrate maltodextrin to the concentrate, mix evenly, and spray dry to obtain the instant wolfberry powder; the enzyme is cellulase: pectinase prepared in a mass ratio of (4-6): (1-2). The instant wolfberry powder is a wolfberry extract with a polysaccharide content of ≥2%.

5. A composition having both brain-boosting and vision-enhancing effects as claimed in claim 4, characterized in that: The polysaccharide content in the wolfberry instant powder is ≥2wt.%.

6. A composition having both brain-boosting and vision-enhancing effects as claimed in claim 1, 2 or 3, characterized in that: The blueberry powder preparation method comprises the following steps: using fresh blueberries as raw materials to prepare a pulp that can pass through a 20-mesh sieve, adding 0.2%-5% enzyme by weight of fresh blueberries, stirring evenly, heating the pulp to 40-60° C. and maintaining for 1-2 hours, heating the pulp to boiling, centrifuging and filtering, taking the filtrate and concentrating it under reduced pressure at 80° C., adding 30%-45% maltodextrin by weight of the concentrate, and spray drying the concentrate to obtain the blueberry powder; the blueberry powder is prepared by mixing cellulase and pectinase in a mass ratio of (1-2): (4-6).

7. A composition having both brain-boosting and vision-enhancing effects as claimed in claim 1, 2 or 3, characterized in that: The preparation technology of the DHA algae oil powder is based on DHA algae oil with a DHA content of at least 50%. According to the mass ratio, whey protein powder, 30%-36% maltodextrin, 5%-7% sodium caseinate, 6%-8% citric acid fatty acid glyceride, 0.1%-1% sodium ascorbate of DHA algae oil 80%-85% are taken, whey protein powder, maltodextrin, sodium caseinate, citric acid fatty acid glyceride and sodium ascorbate are dissolved in twice the amount of 55°C-65°C warm water, 10000rpm-15000rpm emulsification 10min-15min, then DHA algae oil is added, 10000rpm-15000rpm emulsification 15min-30min, 50Mpa-60Mpa homogenization 2-3 times, spray drying. The DHA algae oil powder obtained by this preparation technology has better stability, water solubility and fluidity, which is convenient for product processing.

8. The application of a composition having both brain-boosting and vision-enhancing effects as described in claim 1, 2 or 3, wherein the DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder and blueberry fruit powder are dissolved in pure water, mixed evenly, filtered and packaged to obtain a liquid beverage product having both brain-boosting and vision-enhancing effects.

9. The application of a composition having both brain-boosting and vision-benefiting effects as described in claim 1, 2 or 3, comprising mixing the DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder and blueberry powder, adding cereal powder, mixing evenly and packaging to obtain a dispersible cereal product having both brain-boosting and vision-benefiting effects.

10. The application of a composition having both brain-boosting and vision-benefiting effects as described in claim 1, 2 or 3, wherein the DHA algae oil powder, walnut peptide, lutein ester, tea theanine, wolfberry instant powder and blueberry fruit powder are mixed, and after adding suitable additives, a granule, capsule, tablet, powder, ointment, pill or oral liquid is prepared.

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