Portable phenylalanine detector and use method thereof

Abstract

The invention discloses a portable phenylalanine detector and a use method thereof, and belongs to the technical field of phenylalanine detection equipment.The portable phenylalanine detector comprises a liquid chromatograph body, the liquid chromatograph body comprises a plurality of detection units, the upper side wall of the detection unit on the uppermost side is fixedly connected with a pressure pump, and the upper side wall of the detection unit on the uppermost side is fixedly connected with a pressure sensor; the water outlet end of the pressure pump is fixedly connected with a liquid inlet pipe communicated with the uppermost detection unit, the upper side wall of the uppermost detection unit is fixedly connected with a storage box, the storage box is divided into a water storage area and a sample area, and cleaning water and a sample storage bottle are arranged in the water storage area and the sample area respectively. A conveying pipe is fixedly connected between every two adjacent detection units, and a liquid discharging pipe is fixedly connected to the detection unit on the lowermost side. And through the one-by-one detection assembly, the worker is helped to quickly deduce which section of pipeline and the adjacent detection unit are blocked.

Description

Technical Field

[0001] The invention relates to the technical field of phenylalanine detection equipment, in particular to a portable phenylalanine detector and a use method thereof. Background Art

[0002] Phenylalanine is an amino acid, one of the essential amino acids in the human body and one of the important components of protein. In the human body, phenylalanine can also be converted into important neurotransmitters such as dopamine, norepinephrine and adrenaline, which plays an important role in the nervous system and physical health. Phenylalanine can be obtained through food intake and is mainly found in meat, beans, nuts, dairy products and grains. Lack of phenylalanine metabolizing enzymes in the body leads to excessive accumulation of phenylacetone in the body, which will cause the patient's body to be unable to metabolize phenylalanine normally, resulting in phenylalanine being converted into phenylacetone, and then phenylacetone is further converted into harmful metabolites in the body, causing the patient to suffer from phenylketonuria. Phenylketonuria is a hereditary metabolic disease. Since the early symptoms of phenylketonuria include mental retardation, skin rash, sparse hair, abnormal behavior, etc., if not treated in time, it may have a serious impact on brain development. In order to detect whether young children have phenylketonuria, it is necessary to fully mix the urine of the young children with the detection agent in the phenylalanine detection kit, and then use a high-performance liquid chromatography to detect the components, so as to detect symptoms in time and treat them in time.

[0003] A Chinese patent discloses a method for detecting the chiral purity of [(aminocarbonyl)amino]-N-[fluorenylmethoxycarbonyl]-D-phenylalanine (authorization announcement number CN117554522A). The patent discloses a method for detecting the chiral purity of 4-[(aminocarbonyl)amino]-N-[fluorenylmethoxycarbonyl]-D-phenylalanine, which mainly solves the technical problems that the isocratic method cannot achieve baseline separation. The determination method of the present invention comprises the following steps: first, preparation of test reagents; second, sample preparation; third, high performance liquid chromatography chiral detection, mainly using the high resolution of liquid chromatograph-diode array detector (DAD), two-step gradient and Dacel chiral column to detect and separate isomers. The present invention realizes the simultaneous detection of the sample to be tested without interference, and provides a reliable and convenient detection means for the chiral detection of 4-[(aminocarbonyl)amino]-N-[fluorenylmethoxycarbonyl]-D-phenylalanine.

[0004] However, when the patent uses a liquid chromatograph for detection, the liquid chromatograph performs multi-stage processing on the sample, including vacuum degassing, sample injection, sample separation, sample detection and signal output. During this process, impurities in the sample are easily blocked inside the pipeline. Once blockage occurs, the staff needs to dismantle and check the multi-stage components one by one to find the blocked part and finally clean or replace it, which is very inconvenient. Especially when blockage occurs in special occasions such as outdoor use, it is difficult to carry out portable inspection and repair. For this reason, the applicant proposes a portable phenylalanine detector and a method of using it. Summary of the invention

[0005] The object of the present invention is to provide a portable phenylalanine detector and a method of using the same to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions: A portable phenylalanine detector and a method for using the same, comprising a liquid chromatograph body, the liquid chromatograph body comprising a plurality of detection units, a pressure pump being fixedly connected to the upper side wall of the uppermost detection unit, a liquid inlet pipe communicating with the uppermost detection unit being fixedly connected to the water outlet end of the pressure pump, a storage box being fixedly connected to the upper side wall of the uppermost detection unit, the storage box being divided into a water storage area and a sample area, clean water and a sample storage bottle being respectively arranged inside the water storage area and the sample area, a transmission pipe being fixedly connected between two adjacent detection units, a liquid discharge pipe being fixedly connected to the lowermost detection unit, a reflux pipe being fixedly connected to the water inlet end of the pressure pump, a pressure sensor being installed on the liquid inlet pipe, individual detection components for detecting blockage being installed between the plurality of transmission pipes, and a flow direction regulating component being installed on the liquid inlet pipe.

[0007] As a further solution of the present invention, each detection component includes a transverse tube fixedly connected to the transmission tube, a movable plug is slidably connected inside the transverse tube, a reset spring is fixedly connected between the movable plug and the transverse tube, a movable cylinder is slidably connected inside the transverse tube, and a movable adjustment component is fixedly connected between the movable cylinder and the movable plug.

[0008] As a further solution of the present invention, a same flow pipe is fixedly connected between multiple transverse tubes, a same sliding box is fixedly connected to one end of multiple transverse tubes away from the transmission tube, trapezoidal blocks located inside the sliding box are fixedly connected to multiple moving cylinders, and a pushing block is slidably connected inside the sliding box.

[0009] As a further solution of the present invention, the flow direction regulating component includes a valve housing fixedly connected to the liquid inlet pipe, a valve core is rotatably connected inside the valve housing, two first valve channels arranged obliquely and symmetrically are bored on the valve core, a second valve channel is bored on the valve core, and a fixed cylinder is fixedly connected to the upper end of the valve housing.

[0010] As a further solution of the present invention, the upper end of the valve core is fixedly connected to a seal that is rotatably connected to a fixed tube, the seal is rotatably connected to a backflow pipe, a backflow valve assembly is fixedly connected between the backflow pipe and the flow pipe, the seal is fixedly connected to a rotating disk, and the lower end of the valve core is rotatably connected to a return pipe that is connected to the water storage area of ​​the storage box.

[0011] As a further solution of the present invention, the reverse flow valve assembly includes a reverse flow tube fixedly connected between the through-flow pipe and the reverse flow pipe, a reverse flow plug is slidably connected inside the reverse flow tube, and an L-shaped hole is drilled inside the reverse flow plug.

[0012] As a further solution of the present invention, a connecting spring is fixedly connected between the backflow plug and the upper inner wall of the backflow cylinder.

[0013] As a further solution of the present invention, the movable adjustment component includes a limit box fixedly connected to the movable plug, and the upper and lower side walls of the limit box are slidably connected with an insertion rod inserted into the horizontal tube.

[0014] As a further solution of the present invention, a tension spring is fixedly connected between the insertion rod and the limit box, a T-bar is fixedly connected inside the moving cylinder, and connecting ropes are fixedly connected between the two insertion rods and the T-bar.

[0015] As a further solution of the present invention, a method for using a portable phenylalanine detector comprises the following steps: Step 1, placing the urine of the child and the phenylalanine detection agent into the sample storage bottle respectively, connecting the reflux pipe with the sample storage bottle, and starting the pressure pump to detect the sample to be tested formed by mixing the urine and the phenylalanine detection agent; Step 2: The staff detects the pressure state inside the liquid inlet pipe through a computer and a pressure sensor. When the computer shows that the pressure inside the liquid inlet pipe is too high, it means that the pipe is blocked; Step 3: The staff moves the push block to inspect the multiple transmission pipes respectively, and observes the pressure state inside the liquid inlet pipe through the computer to determine which specific detection unit is blocked; Step 4: After the staff rotates the rotating disk 90 degrees and connects the reflux pipe to the water storage area in the storage box, they start the pressure pump to backwash the blocked transmission pipe and detection unit until the computer shows that the pressure inside the liquid inlet pipe is in normal state.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. When the present invention is used, it can quickly determine whether the pipeline and the detection unit of the device are blocked through the liquid inlet pipe, and help the staff to quickly infer which section of the pipeline and its adjacent detection unit are blocked by detecting the components one by one.

[0017] 2. When the present invention is used, the flow direction of the pipeline is quickly changed through the flow direction regulating component, thereby achieving the effective backwashing purpose of the blocked pipeline and its adjacent detection unit, and can flush out the blocked impurities to a certain extent. Compared with the existing method of checking and cleaning one by one, the device is easy to detect and more convenient to maintain.

[0018] 3. When the present invention is used, the movable adjustment component can effectively prevent the clean water from being discharged into other unblocked transmission pipes and adjacent detection units under the action of pressure, thereby affecting the pressure sensor's detection of pressure. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A three-dimensional image of the present application; Figure 2 A schematic diagram of the structure of each component part of the application; Figure 3 This is a schematic diagram of the structure of the pressure pump part of this application; Figure 4 This is a schematic diagram of the structure of the flow direction regulating component of this application; Figure 5 This is a schematic diagram of the structure of the mobile adjustment component of this application; Figure 6 This is a schematic diagram of the structure of the reverse flow valve assembly of this application; Figure 7 The state diagram when testing components one by one for this application; Figure 8 for Figure 7 The enlarged view of point A in the middle; Fig. 9 This is a flow chart of the method of use of this application.

[0020] In the figure: 1. Liquid chromatograph body; 101. Detection unit; 102. Pressure pump; 103. Liquid inlet pipe; 104. Storage box; 105. Sample storage bottle; 106. Transmission pipe; 107. Liquid discharge pipe; 108. Reflux pipe; 109. Pressure sensor; 2. Detect components one by one; 201, horizontal tube; 202, moving plug; 203, return spring; 204, moving cylinder; 205, flow tube; 206, sliding box; 207, trapezoidal block; 208, push block; 3. Mobile adjustment component; 301. Limit box; 302. Insertion rod; 303. Tension spring; 304. T-bar; 305. Connecting rope; 4. Flow direction regulating assembly; 401. Valve housing; 402. Valve core; 403. First valve channel; 404. Second valve channel; 405. Fixed cylinder; 406. Sealing element; 407. Backflow pipe; 408. Rotating disk; 409. Return pipe; 5. Backflow valve assembly; 501. Backflow cylinder; 502. Backflow plug; 503. L-shaped hole; 504. Connecting spring. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Embodiment 1 See also Figures 1 to 6In an embodiment of the present invention, a portable phenylalanine detector and a method of using the same include a liquid chromatograph body 1, the liquid chromatograph body 1 includes five detection units 101 for detecting samples, the five detection units 101 include a vacuum degassing unit, a chromatographic column unit, an injector unit, a detector unit, and a data recording unit, respectively. The structures and principles of the detection units 101 are well-known to those skilled in the art, and will not be described in detail herein. The upper side wall of the uppermost detection unit 101 is fixedly connected to a pressure pump 102 by bolts, and the pressure pump 102 is a constant flow plunger pump capable of providing a constant flow rate. The structures and principles of the pressure pump 102 are well-known to those skilled in the art, and will not be described in detail herein. The water outlet of the pressure pump 102 is fixedly connected with a liquid inlet pipe 103 connected with the uppermost detection unit 101. The upper side wall of the uppermost detection unit 101 is fixedly connected with a storage box 104 by bolts. The storage box 104 is divided into a water storage area for storing clean water and a sample area for storing a sample bottle 105 by a partition fixed at the center thereof. Clean water and a sample bottle 105 are respectively arranged inside the water storage area and the sample area. A transmission pipe 106 is fixedly connected between two adjacent detection units 101 by a flange. A discharge pipe 107 is fixedly connected to the lowermost detection unit 101. When the reflux pipe 108 is connected to the sample bottle 105, the staff starts the pressure pump 1 02, the infant urine and phenylalanine detection agent are mixed and drawn in, and then enter the uppermost detection unit 101 through the liquid inlet pipe 103 for vacuum degassing, and then discharged into the second detection unit 101 through the transmission pipe 106 for separation process, and then discharged into the third detection unit 101 for injection process, and then discharged into the fourth detection unit 101 for detection process, and finally discharged into the fifth detection unit 101 for data processing process, and then discharged from the liquid discharge pipe 107 to achieve the purpose of sample detection. When one of the transmission pipes 106 or the detection unit 101 is blocked, the pressure sensor 109 detects that the pressure inside the liquid inlet pipe 103 is too high. , and the numerical value is displayed on the computer. The water inlet end of the pressure pump 102 is fixedly connected to the reflux pipe 108 through a flange. When the reflux pipe 108 detects the sample, it is necessary to connect the reflux pipe 108 with the sample storage bottle 105. A pressure sensor 109 is installed on the liquid inlet pipe 103. The pressure sensor 109 is electrically connected to the computer through a signal line to achieve the purpose of pressure detection, so as to determine whether a blockage occurs at present. The structure and principle of the pressure sensor 109 are well known to those skilled in the art and will not be repeated here. A detection component 2 for detecting blockage is installed one by one between the multiple transmission pipes 106, and a flow direction regulating component 4 for regulating the flow direction of the liquid is installed on the liquid inlet pipe 103.

[0023] Each detection component 2 includes a transverse tube 201 fixedly connected to a middle section of the transmission tube 106, a movable plug 202 is slidably connected inside the transverse tube 201, and a through hole matching the transmission tube 106 is drilled on the movable plug 202. In the initial state, the through hole of the movable plug 202 corresponds to the transmission tube 106, so that the purpose of detecting the sample can be achieved. A reset spring 203 is fixedly connected between the movable plug 202 and the transverse tube 201. In the initial state, the transverse tube 201 is in a stress-free state. A movable cylinder 204 is slidably connected inside the transverse tube 201, and an elliptical through hole is drilled on the movable cylinder 204, so that the movable cylinder 204 can maintain a state of communication with the flow tube 205 when moving. A movable adjustment component 3 of the detection unit 101 for preventing clean water from flowing to an unblocked state is fixedly connected between the movable cylinder 204 and the movable plug 202.

[0024] A common flow pipe 205 is fixedly connected between the multiple transverse tubes 201, and a common sliding box 206 is fixedly connected to one end of the multiple transverse tubes 201 away from the transmission tube 106. A trapezoidal block 207 located inside the sliding box 206 is fixedly connected to the multiple moving cylinders 204. The front and rear side walls of the trapezoidal block 207 and the front and rear side inner walls of the sliding box 206 are connected by sliding grooves to achieve lateral sliding. A pushing block 208 is slidably connected inside the sliding box 206, and a handle is fixedly connected to the pushing block 208. A sliding hole matching the handle is drilled on the sliding box 206. When the pushing block 208 moves downward, the trapezoidal block 207 can be driven to move horizontally through its inclined surface.

[0025] The flow direction regulating component 4 includes a valve housing 401 fixedly connected to the liquid inlet pipe 103, and a valve core 402 is rotatably connected to the inside of the valve housing 401 through a sealing member 406. Two obliquely symmetrically arranged first valve channels 403 are opened on the valve core 402. The water outlet of the pressure pump 102 can be connected to the countercurrent pipe 407 through the two first valve channels 403. In addition, the liquid inlet pipe 103 can be connected to the return pipe 409. This state is used for backwashing. A second valve channel 404 is opened on the valve core 402. After the valve core 402 is rotated 90 degrees, the water outlet of the pressure pump 102 can be connected only to the liquid inlet pipe 103. This state is used for testing samples. A fixing cylinder 405 is fixedly connected to the upper end of the valve housing 401.

[0026] The upper end of the valve core 402 is fixedly connected to a seal 406 rotatably connected to a fixed cylinder 405. The seal 406 can ensure that when the valve core 402 rotates, there will be no leakage at the connection with the backflow pipe 407. The seal 406 is rotatably connected to the backflow pipe 407. A backflow valve assembly 5 for transmitting cleaning water during backwashing is fixedly connected between the backflow pipe 407 and the flow pipe 205. A rotating disk 408 is fixedly connected to the seal 406. The lower end of the valve core 402 is rotatably connected to a return pipe 409 connected to the water storage area of ​​the storage box 104, so that the cleaning water after backwashing can be discharged back into the storage box 104. It is worth noting that a filter screen needs to be installed at the connection between the storage box 104 and the return pipe 409 to prevent impurities inside it from flowing into the water storage area of ​​the storage box 104 during backwashing.

[0027] The reverse flow valve assembly 5 includes a reverse flow cylinder 501 fixedly connected between the flow pipe 205 and the reverse flow pipe 407, a reverse flow plug 502 is slidably connected inside the reverse flow cylinder 501, and an L-shaped hole 503 is drilled inside the reverse flow plug 502. When the reverse flow pipe 407 discharges clean water, the pressure of the pressure pump 102 is used to push the reverse flow plug 502 downward to achieve the connection between the reverse flow pipe 407 and the flow pipe 205, so that the flow pipe 205 can be used for backwashing. When the state of the sample is detected, the reverse flow plug 502 is pulled by the connecting spring 504 so that the reverse flow pipe 407 will not be connected to the flow pipe 205, so that the detection assembly 2 can be conveniently checked for blocked areas one by one, and clean water can be discharged to the water storage area of ​​the storage box 104 through the flow pipe 205. A connecting spring 504 is fixedly connected between the reverse flow plug 502 and the upper inner wall of the reverse flow cylinder 501.

[0028] Embodiment 2 See also Figures 7 to 9 Based on Example 1, the movable adjustment component 3 includes a limit box 301 fixedly connected to the movable plug 202 by welding, and the upper and lower side walls of the limit box 301 are slidably connected with a plug rod 302 plugged into the cross tube 201, and the upper and lower inner walls of the cross tube 201 are both provided with holes matching the plug rod 302. It is worth noting that in order to prevent the plug rod 302 from entering the interior of the transmission tube 106 when the limit box 301 is reset, resulting in the inability to reset the interior of the limit box 301, a horizontal plate can be set at the port of the transmission tube 106 to resist the plug rod 302.

[0029] A tension spring 303 is fixedly connected between the insertion rod 302 and the limit box 301, and a spring plate is installed at the opposite end of the two insertion rods 302, and the tension spring 303 is fixed by the spring plate. A T-bar 304 is fixedly connected inside the moving cylinder 204, and a connecting rope 305 is fixedly connected between the two insertion rods 302 and the T-bar 304. When the moving cylinder 204 moves, the connecting rope 305 can be pushed to make the connecting rope 305 pull the two insertion rods 302 closer to each other, so as to release the limit of the limit box 301, and push the mobile plug 202 to move by continuing to push the T-bar 304, so as to effectively prevent the mobile plug 202 from being displaced by water pressure in the backwash state, causing clean water to enter the detection unit 101 in the unblocked area.

[0030] Step 1, placing the urine and phenylalanine detection agent of the child into the sample storage bottle 105 respectively, and connecting the reflux pipe 108 with the interface of the sample storage bottle 105, starting the pressure pump 102, extracting the urine and the phenylalanine detection agent, during the extraction process, the urine and the phenylalanine detection agent are mixed to form a sample to be tested, and the sample to be tested is transmitted to the multiple detection units 101 through the liquid inlet pipe 103 and the transmission pipe 106 for detection; Step 2: The staff detects the pressure state inside the liquid inlet pipe 103 through the computer and the pressure sensor 109. When the computer shows that the pressure inside the liquid inlet pipe 103 is too high, it means that the sample to be tested is blocked during transmission, that is, the pipeline is blocked; Step 3, the staff moves the push block 208 to drive the trapezoidal block 207 corresponding to the transmission tube 106 to be tested to move, so that the transmission tube 106 is connected with the flow tube 205, so as to achieve the purpose of testing multiple transmission tubes 106 respectively, and observe the pressure state inside the liquid inlet tube 103 through the computer to determine which specific detection unit 101 is blocked; Step 4, the staff rotates the rotating disk 408 ninety degrees to connect the water outlet of the pressure pump 102 with the counterflow pipe 407, the liquid inlet pipe 103 with the return pipe 409, and connects the return pipe 108 with the water storage area in the storage box 104. Then, by starting the pressure pump 102, the blocked part of the transmission pipe 106 and the detection unit 101 are backwashed, and the blocked impurities are flushed into the filter net in the water storage area of ​​the storage box 104 until the computer shows that the pressure inside the liquid inlet pipe 103 is in a normal state.

[0031] The working principle of the present invention is: When the sample needs to be tested, the present invention first injects the infant urine and the phenylalanine detection agent into the sample storage bottle 105 respectively, then docks the reflux pipe 108 with the sample storage bottle 105, and rotates the rotating disk 408 so that the water outlet of the pressure pump 102 is connected with the liquid inlet pipe 103 through the second valve channel 404. At this time, the pressure pump 102 is started to mix the infant urine and the phenylalanine detection agent and then enter the uppermost detection unit 101 through the liquid inlet pipe 103 for vacuum degassing, and then discharges into the second detection unit 101 through the transmission pipe 106 for separation process, then discharges into the third detection unit 101 for injection process, then discharges into the fourth detection unit 101 for detection process, and finally discharges into the fifth detection unit 101 for data processing process, and then discharges from the discharge pipe 107 to achieve the purpose of sample testing; When one of the transmission pipes 106 or the detection unit 101 is blocked, the pressure sensor 109 detects that the pressure inside the liquid inlet pipe 103 is too high and displays the value on the computer. At this time, the staff can connect the return pipe 108 with the water storage area of ​​the storage box 104 to make the pressure pump 102 extract the clean water inside the storage box 104 and move the push block 208 downward. When the push block 208 contacts the trapezoidal block 207, the trapezoidal block 207 is driven to move horizontally through its inclined surface, and the trapezoidal block 207 drives the moving cylinder 108 to move horizontally. 204 moves, the moving cylinder 204 pushes the connecting rope 305 through the T-bar 304, so that the connecting rope 305 pulls the two plug rods 302 closer to each other, thereby releasing the limit of the limit box 301, and the T-bar 304 continues to push, driving the moving plug 202 to move. At this time, the pressure pump 102 extracts the clean water in the water storage area of ​​the storage box 104 and discharges it from the transmission pipe 106. If the discharge is successful and the pressure sensor 109 detects that the pressure at the liquid inlet pipe 103 is normal, it means that the transmission pipe 106 at this location is in a connected state; It is worth noting that when the movable plug 202 is produced, the bottom of the movable plug 202 needs to be lengthened so that after the movable plug 202 moves, only the transmission tube 106 on the upper side of the transverse tube 201 can be connected to the transverse tube 201, and the transmission tube 106 on the lower side of the transverse tube 201 is not connected to the transverse tube 201 due to the lengthening of the movable plug 202.

[0032] When it is detected that the transmission tube 106 is in a connected state, the clean water and part of the sample are discharged into the water storage area of ​​the storage box 104 through the flow tube 205. At this time, the staff can continue to move the push block 208 downward to detect the next transmission tube 106 and the detection unit 101. After the push block 208 moves downward, the previous trapezoidal block 207 will be reset under the thrust of the reset spring 203, so that the movable plug 202 is connected to the transmission tube 106 again, and the insertion rod 302 in the movable adjustment component 3 will be reset and inserted into the horizontal tube 201 again; at this time, the clean water will continue to flow to the next transmission tube 106 and the detection unit 101 under the power of the pressure pump 102; When the transmission pipe 106 and the detection unit 101 of the next section are detected to be blocked, the pressure sensor 109 will first detect that the pressure at the liquid inlet pipe 103 becomes larger, and the staff can activate the backwashing function at this time; First, the staff rotates the rotating disk 408 by 90 degrees, so that the water outlet of the pressure pump 102 is connected to the counterflow pipe 407, and the liquid inlet pipe 103 is connected to the return pipe 409. At this time, the pressure pump 102 extracts clean water from the water storage area of ​​the storage box 104 and transmits the clean water to the counterflow pipe 407. Then the clean water passes through the counterflow cylinder 501, and its water pressure pushes the counterflow plug 502 downward, so that the clean water is transmitted to the through-flow pipe 205 and is in a reverse flow state in the through-flow pipe 205. Since the transmission pipe 106 of the blocked section is connected to the through-flow pipe 205, At this time, the clean water will directly enter the transmission pipe 106 of this section and flow upward to backwash the impurities in the transmission pipe 106 and the detection unit 101. When the impurity flushing is completed, the blocked impurities will enter the first valve channel 403 of the valve core 402 through the liquid inlet pipe 103, and then be flushed from the first valve channel 403 through the return pipe 409 to the filter screen in the water storage area of ​​the storage box 104. At this time, the computer shows that the pressure inside the liquid inlet pipe 103 is in a normal state. In addition, the staff can also adjust the power of the pressure pump 102 to improve the backwashing function.

[0033] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A portable phenylalanine detector, comprising a liquid chromatograph body (1), characterized in that: The liquid chromatograph body (1) comprises a plurality of detection units (101); a pressure pump (102) is fixedly connected to the upper side wall of the uppermost detection unit (101); a water outlet of the pressure pump (102) is fixedly connected to a liquid inlet pipe (103) which is in communication with the uppermost detection unit (101); a storage box (104) is fixedly connected to the upper side wall of the uppermost detection unit (101); the storage box (104) is divided into a water storage area and a sample area; clean water and a storage area are respectively arranged inside the water storage area and the sample area. A sample bottle (105), a transmission tube (106) is fixedly connected between two adjacent detection units (101), a discharge tube (107) is fixedly connected to the lowermost detection unit (101), a return tube (108) is fixedly connected to the water inlet end of the pressure pump (102), a pressure sensor (109) is installed on the liquid inlet tube (103), and detection components (2) for detecting blockage are installed one by one between multiple transmission tubes (106), and a flow direction regulating component (4) is installed on the liquid inlet tube (103).

2. A portable phenylalanine detector according to claim 1, characterized in that: The one-by-one detection assembly (2) comprises a transverse tube (201) fixedly connected to the transmission tube (106); a movable plug (202) is slidably connected inside the transverse tube (201); a return spring (203) is fixedly connected between the movable plug (202) and the transverse tube (201); a movable cylinder (204) is slidably connected inside the transverse tube (201); and a movable adjustment assembly (3) is fixedly connected between the movable cylinder (204) and the movable plug (202).

3. A portable phenylalanine detector according to claim 2, characterized in that: A common flow pipe (205) is fixedly connected between the plurality of transverse tubes (201), one end of the plurality of transverse tubes (201) away from the transmission tube (106) is fixedly connected to a common sliding box (206), a trapezoidal block (207) located inside the sliding box (206) is fixedly connected to each of the plurality of moving cylinders (204), and a pushing block (208) is slidably connected inside the sliding box (206).

4. A portable phenylalanine detector according to claim 1, characterized in that: The flow direction regulating assembly (4) comprises a valve housing (401) fixedly connected to the liquid inlet pipe (103), a valve core (402) being rotatably connected inside the valve housing (401), two first valve channels (403) being obliquely symmetrically arranged are bored on the valve core (402), a second valve channel (404) is bored on the valve core (402), and a fixing cylinder (405) is fixedly connected to the upper end of the valve housing (401).

5. A portable phenylalanine detector according to claim 4, characterized in that: The upper end of the valve core (402) is fixedly connected to a sealing member (406) rotatably connected to a fixed cylinder (405); a counterflow pipe (407) is rotatably connected to the sealing member (406); a counterflow valve assembly (5) is fixedly connected between the counterflow pipe (407) and the flow pipe (205); a rotating disk (408) is fixedly connected to the sealing member (406); and a return pipe (409) is rotatably connected to the lower end of the valve core (402) and is connected to a water storage area of ​​the storage box (104).

6. A portable phenylalanine detector according to claim 5, characterized in that: The reverse flow valve assembly (5) comprises a reverse flow cylinder (501) fixedly connected between the throughflow pipe (205) and the reverse flow pipe (407), a reverse flow plug (502) being slidably connected inside the reverse flow cylinder (501), and an L-shaped hole (503) is bored inside the reverse flow plug (502).

7. A portable phenylalanine detector according to claim 6, characterized in that: A connecting spring (504) is fixedly connected between the backflow plug (502) and the upper inner wall of the backflow cylinder (501).

8. A portable phenylalanine detector according to claim 2, characterized in that: The movable adjustment assembly (3) comprises a limit box (301) fixedly connected to the movable plug (202), and upper and lower side walls of the limit box (301) are slidably connected to an insertion rod (302) inserted into the transverse tube (201).

9. A portable phenylalanine detector according to claim 8, characterized in that: A tension spring (303) is fixedly connected between the insertion rod (302) and the limit box (301), a T-bar (304) is fixedly connected inside the moving cylinder (204), and a connecting rope (305) is fixedly connected between the two insertion rods (302) and the T-bar (304).

10. A method for using a portable phenylalanine detector, characterized in that: The steps include: Step 1, placing the urine of the child and the phenylalanine detection agent into the sample storage bottle (105) respectively, connecting the reflux pipe (108) to the sample storage bottle (105), and starting the pressure pump (102) to detect the sample to be tested formed by mixing the urine and the phenylalanine detection agent; Step 2: The staff detects the pressure state inside the liquid inlet pipe (103) through a computer and a pressure sensor (109). When the computer displays that the pressure inside the liquid inlet pipe (103) is too high, it indicates that the pipe is blocked; Step 3: The staff member moves the push block (208) to inspect the multiple transmission tubes (106) respectively, and observes the pressure state inside the liquid inlet tube (103) through a computer to determine which specific detection unit (101) is blocked; Step 4: The staff rotates the rotating disk (408) 90 degrees and docks the return pipe (108) with the water storage area in the storage box (104), and then starts the pressure pump (102) to backwash the blocked part of the transmission pipe (106) and the detection unit (101) until the computer shows that the pressure inside the liquid inlet pipe (103) is in a normal state.

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