Method for promoting germination of gelsmium elegans seeds
By using seed soaking agent of sodium nitrophenol and gibberellin, and cultured under specific conditions, the problems of low germination rate and long seed emergence of the stored seeds were solved, and the seeds were achieved Efficient germination and rapid reproduction.
Patent Information
- Application Number
- CN202411608487.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-02-18
AI Technical Summary
The germination seeds after storage have low germination rate and long emergence time, which affects their rapid reproduction and standardized planting and production.
The seeds were soaked with seed soaking agent containing sodium nitrophenol and gibberellin, and cultured in a constant temperature incubator to control temperature, humidity and light conditions.
It significantly improves the germination rate and early germination time of the hook-knot seeds, and promotes rapid reproduction and uniform growth of the seeds.
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Figure CN119452805A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of Gelsemium elegans seed germination, and more particularly to a method for promoting Gelsemium elegans seed germination. Background Art
[0002] Gelsemium elegans (Gardn.et Champ.) Benth., also known as wild kudzu, vine, and broken intestine grass, is a plant of the genus Gelsemium in the Loganiaceae family. It was first recorded in Shennong's Herbal Classic and was listed as a low-grade product. It is one of the world's most famous highly toxic Chinese medicines, with the effects of treating wind and cold, reducing swelling and relieving pain, and removing toxins and killing insects. Modern pharmacological studies have shown that Gelsemium elegans has the effects of immunomodulation, anti-tumor, analgesia and sedation, promoting hematopoiesis, lowering blood pressure, and dilating pupils. Because Gelsemium elegans is highly toxic, it has been mainly used externally by the people, and is not taken internally. It is mostly crushed or ground and applied to the affected area, or it can be boiled in water for washing or fumigation. It has the functions of dispelling wind and cold, reducing swelling and relieving pain, and attacking toxins and killing insects. At present, the collection of Gelsemium elegans is mainly based on wild resources, and its reproduction mainly relies on ramet reproduction and seed reproduction. The multiplication coefficient of ramet reproduction is low, and the survival rate is affected by many factors; while the germination rate of seed reproduction is low, and the time required for germination is long, especially after storage, the germination rate and germination time of Gelsemium elegans seeds are affected, which affects the promotion and use. Therefore, it is necessary to find a method to promote the germination of Gelsemium elegans seeds, so as to improve the germination rate of Gelsemium elegans seeds and advance the germination time, which is conducive to the rapid reproduction of a large number of seedlings, solve the problem of seedling shortage, and provide a favorable basis for the standardized planting and production of Gelsemium elegans. Summary of the invention
[0003] The invention aims to provide a method for promoting the germination of Gelsemium elegans seeds, so as to solve the technical problems of low germination rate and long emergence time of Gelsemium elegans seeds after storage.
[0004] In order to achieve the purposes of the present invention, the present invention provides a method for promoting the germination of Gelsemium elegans seeds, comprising: The seeds of Gelsemium elegans are soaked in a seed soaking agent for 50-120 min, wherein the seed soaking agent contains 10-30 mg / L sodium nitrophenolate and 30-60 mg / L gibberellin, and the rest is pure water; The treated Gelsemium elegans seeds are sown in the germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 20-32°C, the humidity of the incubator is 75-85%, the light intensity is 2000-4000lx, the light period is 12h, the dark period is 12h, and the cultivation time is 7-30d.
[0005] Preferably, before the soaking treatment with the seed soaking agent, the method further comprises: Step 1: Soak the air-dried Gelsemium seeds stored at room temperature in tap water for 20-30 min, then place the Gelsemium seeds and the soaking water in a 40-60℃ water bath for 3-5 h, and then place in a low temperature environment of 3-4℃ for 4-5 h, alternating between the water bath and the low temperature environment 2-3 times; Step 2: Filter out the Gelsemium elegans seeds, bring to room temperature, and disinfect them.
[0006] Preferably, the Gelsemium seeds are air-dried and stored at room temperature for 2-4 months, the germination matrix comprises a seedling matrix: vermiculite = 3:1; the amount of seeds sown per square meter is 2-3g, after sowing, a layer of 0.1-0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer, and the fine seedling matrix is passed through an 800-900 mesh sieve. The seedling matrix is prepared from peat soil, perlite, and chicken manure in a weight ratio of 6:2:2.
[0007] Preferably, in step 1, the weight ratio of Gelsemium elegans seeds to tap water is 1:20-60.
[0008] Preferably, the specific steps of disinfection treatment are as follows: Gelsemium elegans seeds are soaked in a 0.1% by mass detergent aqueous solution for 20 minutes, then soaked in 75% by mass alcohol for 8 minutes, and then washed with pure water for 3-4 times.
[0009] Preferably, the weight ratio of Gelsemium elegans seeds to the detergent aqueous solution, alcohol and seed soaking agent is 1:10-20:10-20:10-20.
[0010] Preferably, during the tap water soaking, disinfection treatment and seed soaking agent soaking processes, a hard mesh plate with a pore size smaller than the Gelsemium seed particle size is placed above the Gelsemium seeds to immerse the Gelsemium seeds in the liquid.
[0011] Preferably, the seed soaking agent also contains 50-80 mg / L of sodium bicarbonate. By adding sodium bicarbonate, sodium nitrophenolate and gibberellin to soak the seeds, the stability and activity of the seed soaking agent can be improved, and the surface and wing of Gelsemium elegans seeds can be made more absorbent and softened, thereby improving the cell activation effect.
[0012] Preferably, during the soaking process of the seed soaking agent, air pipes are evenly laid below the Gelsemium seeds (that is, air pipes are evenly laid at the bottom of the seed soaking agent soaking container), and air holes are evenly arranged on the air pipes, and a mixed gas of ozone and negative hydrogen ions is circulated into the air pipes, and the mixed gas enters from the bottom of the Gelsemium seeds, flows to the top of the Gelsemium seeds, and then is collected and circulated and pumped in from the bottom of the Gelsemium seeds; wherein the ozone concentration of the mixed gas initially introduced is 50-100 mg / L, and the average concentration of the negative hydrogen ion gas is 1-2×10 6 / L, the mass volume ratio of Gelsemium seeds to the mixed gas is 1g:5-10L, the mixed gas pumping flow rate is 0.01-0.05L / s, and the ventilation time is the same as the soaking time of the seed soaking agent. Through the action of a certain concentration of ozone and negative hydrogen ion mixed gas on Gelsemium seeds in water, the contact and absorption of Gelsemium seeds and the seed soaking agent are improved, mitochondrial respiration and energy production are promoted, which is beneficial to promote Gelsemium seed cell division and elongation, and improve the germination vitality of Gelsemium seeds.
[0013] The present invention has at least the following beneficial effects: 1. The present invention can effectively improve the germination rate and promote early germination by soaking the stored Gelsemium elegans seeds with a seed soaking agent containing sodium nitrophenolate and gibberellin.
[0014] 2. The present invention first soaks the seeds in tap water and then bathes them in 40-60°C water, which helps to first allow the seeds to absorb water, and then bathes them in warm water, so that the seeds can receive the water temperature more evenly, avoiding local overstimulation of light Gelsemium seeds, and facilitating synchronous temperature alternation to promote the weakening of tissues around the embryo. After absorption and regulation of seed soaking agents such as sodium nitrophenolate and gibberellin, the weakening of tissues around the embryo is further promoted, resulting in seed coat rupture and radicle extension, thereby promoting seed awakening and germination.
[0015] 3. The present invention can improve the stability and activity of the seed soaking agent by adding sodium bicarbonate, sodium nitrophenolate and gibberellin to soak the seeds, and can also improve the water absorption and softening of the surface and wing of Gelsemium elegans seeds, thereby improving the cell activation effect.
[0016] 4. The present invention uses a certain concentration of ozone and negative hydrogen ion mixed gas in water to act on Gelsemium seeds, thereby increasing the contact and absorption of Gelsemium seeds with the seed soaking agent, promoting mitochondrial respiration and energy production, and promoting Gelsemium seed cell division and elongation, thereby increasing the germination vitality of Gelsemium seeds.
[0017] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 Schematic diagram of Gelsemium seeds that are air-dried and stored at room temperature; Figure 2 middle, Figure 2 a is the germination of Gelsemium elegans seeds on the 13th day after sowing in Comparative Example 1, Figure 2 b is the germination situation of Gelsemium elegans seeds on the 13th day after sowing in Example 6; Figure 3 middle, Figure 3 a is the growth condition of Gelsemium elegans seeds on the 30th day after sowing in Comparative Example 1, Figure 3b is the growth condition of Gelsemium elegans seeds at the 30th day after sowing in Example 6. DETAILED DESCRIPTION
[0019] The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.
[0020] It should be understood that the terms such as "having", "comprising" and "including" used in this article do not exclude the presence or addition of one or more other elements or combinations thereof. It should be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials, unless otherwise specified, can be obtained from commercial channels. Gelsemium elegans seeds are usually harvested in the just mature stage, and after removing impurities, they are washed with sterile water. The seeds with no damage, full grains, uniform texture, and no disease or insect eggs are picked out, air-dried, and stored at room temperature for easy sale. The following experiment was carried out with the same batch of Gelsemium elegans seeds that were dried and stored at room temperature for 3 months.
[0021] Example 1 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1: Store the Gelsemium seeds (such as Figure 1 As shown), soaking the seeds in a soaking agent for 50 minutes, wherein the soaking agent contains 10 mg / L sodium nitrophenolate and 30 mg / L gibberellin, and the rest is pure water; Step 2: Sow the treated Gelsemium elegans seeds in a germination matrix and place them in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 20°C, the humidity of the incubator is 75%, the light intensity is 2000x, the light period is 12h, the dark period is 12h, and the cultivation is 30d.
[0022] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds sown per square meter is 2g, and after sowing, a layer of 0.1mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer, and the fine seedling matrix is passed through an 800 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a weight ratio of 6:2:2.
[0023] Among them, the weight ratio of Gelsemium elegans seeds to seed soaking agent is 1:10.
[0024] During the soaking process of the seed soaking agent, a hard mesh plate with a hole diameter smaller than the particle diameter of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0025] Example 2 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1, soaking the Gelsemium elegans seeds that have been air-dried and stored at room temperature for 3 months in a seed soaking agent for 100 minutes, wherein the seed soaking agent contains 15 mg / L sodium nitrophenolate and 40 mg / L gibberellin, and the rest is pure water; Step 2: The treated Gelsemium elegans seeds are sown in a germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 25° C., the humidity of the incubator is 80%, the light intensity is 3000x, the light period is 12 hours, the dark period is 12 hours, and the cultivation is 30 days.
[0026] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 1.5g. After sowing, a layer of 0.15mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through an 850 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0027] Among them, the weight ratio of Gelsemium elegans seeds to seed soaking agent is 1:15.
[0028] During the soaking process of the seed soaking agent, a hard mesh plate with a hole diameter smaller than the particle diameter of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0029] Example 3 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1, soaking the Gelsemium elegans seeds that have been air-dried and stored at room temperature for 3 months in a seed soaking agent for 120 minutes, wherein the seed soaking agent contains 30 mg / L sodium nitrophenolate and 60 mg / L gibberellin, and the rest is pure water; Step 2: Sow the treated Gelsemium elegans seeds in a germination medium and place them in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32° C., the humidity of the incubator is 85%, the light intensity is 4000 lx, the light period is 12 h, the dark period is 12 h, and the cultivation is 30 days.
[0030] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0031] Among them, the weight ratio of Gelsemium elegans seeds to seed soaking agent is 1:20.
[0032] During the soaking process of the seed soaking agent, a hard mesh plate with a hole diameter smaller than the particle diameter of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0033] Example 4 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1, soaking the Gelsemium elegans seeds that have been air-dried and stored at room temperature for 3 months in a seed soaking agent for 120 minutes, wherein the seed soaking agent contains 30 mg / L of sodium nitrophenolate, 60 mg / L of gibberellin and 80 mg / L of sodium bicarbonate, and the rest is pure water; Step 2: Sow the treated Gelsemium elegans seeds in a germination medium and place them in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32° C., the humidity of the incubator is 85%, the light intensity is 4000 lx, the light period is 12 h, the dark period is 12 h, and the cultivation is 30 days.
[0034] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0035] Among them, the weight ratio of Gelsemium elegans seeds to seed soaking agent is 1:20.
[0036] During the soaking process of the seed soaking agent, a hard mesh plate with a hole diameter smaller than the particle diameter of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0037] Example 5 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1: Place the Gelsemium seeds that have been air-dried and stored at room temperature for 3 months into a bottle and add tap water. Soak the seeds in the tap water for 30 min. Then place the seeds and the soaked water in a 60°C water bath for 5 h, and then place them in a 4°C refrigerator for 5 h. Alternate between the water bath and the low temperature environment for 2 times. Step 2, filter out the Gelsemium seeds and bring them to room temperature for disinfection; wherein the specific steps of the disinfection are as follows: soak the Gelsemium seeds in a 0.1% detergent aqueous solution for 20 minutes, then soak them in 75% alcohol for 8 minutes, and then wash them with pure water for 3 times; Step 3, soaking the Gelsemium elegans seeds in a seed soaking agent for 50 minutes, wherein the seed soaking agent contains 30 mg / L of sodium nitrophenolate and 60 mg / L of gibberellin, and the rest is pure water; Step 4: The treated Gelsemium elegans seeds are sown in a germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32° C., the humidity of the incubator is 85%, the light intensity is 4000 lx, the light period is 12 h, the dark period is 12 h, and the cultivation time is 30 days.
[0038] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0039] Wherein, in step 1, the weight ratio of Gelsemium elegans seeds to tap water is 1:60.
[0040] Among them, the weight ratio of Gelsemium elegans seeds to detergent aqueous solution, alcohol and seed soaking agent is 1:20:20:20.
[0041] During the processes of tap water soaking, disinfection treatment, and seed soaking agent soaking, a hard mesh plate with a pore size smaller than the particle size of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0042] Example 6 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1: Place the Gelsemium seeds that have been air-dried and stored at room temperature for 3 months into a bottle and add tap water. Soak the seeds in the tap water for 30 min. Then place the seeds and the soaked water in a 60°C water bath for 5 h, and then place them in a 4°C refrigerator for 5 h. Alternate between the water bath and the low temperature environment for 2 times. Step 2, filter out the Gelsemium seeds and bring them to room temperature for disinfection; wherein the specific steps of the disinfection are as follows: soak the Gelsemium seeds in a 0.1% detergent aqueous solution for 20 minutes, then soak them in 75% alcohol for 8 minutes, and then wash them with pure water for 3 times; Step 3, soaking the Gelsemium elegans seeds in a seed soaking agent for 50 minutes, wherein the seed soaking agent contains 30 mg / L sodium nitrophenolate, 60 mg / L gibberellin and 80 mg / L sodium bicarbonate, and the rest is pure water; Step 4: The treated Gelsemium elegans seeds are sown in a germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32° C., the humidity of the incubator is 85%, the light intensity is 4000 lx, the light period is 12 h, the dark period is 12 h, and the cultivation time is 30 days.
[0043] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0044] Wherein, in step 1, the weight ratio of Gelsemium elegans seeds to tap water is 1:60.
[0045] Among them, the weight ratio of Gelsemium elegans seeds to detergent aqueous solution, alcohol and seed soaking agent is 1:20:20:20.
[0046] During the processes of tap water soaking, disinfection treatment, and seed soaking agent soaking, a hard mesh plate with a pore size smaller than the particle size of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0047] Example 7 A method for promoting the germination of Gelsemium elegans seeds, comprising: Step 1: Place the Gelsemium seeds that have been air-dried and stored at room temperature for 3 months into a bottle and add tap water. Soak the seeds in the tap water for 30 min. Then place the seeds and the soaked water in a 60°C water bath for 5 h, and then place them in a 4°C refrigerator for 5 h. Alternate between the water bath and the low temperature environment for 2 times. Step 2, filter out the Gelsemium seeds and bring them to room temperature for disinfection; wherein the specific steps of the disinfection are as follows: soak the Gelsemium seeds in a 0.1% detergent aqueous solution for 20 minutes, then soak them in 75% alcohol for 8 minutes, and then wash them with pure water 3 times; Step 3, soaking the Gelsemium elegans seeds in a seed soaking agent for 50 minutes, wherein the seed soaking agent contains 30 mg / L sodium nitrophenolate, 60 mg / L gibberellin and 80 mg / L sodium bicarbonate, and the rest is pure water; Step 4: The treated Gelsemium elegans seeds are sown in a germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32° C., the humidity of the incubator is 85%, the light intensity is 4000 lx, the light period is 12 h, the dark period is 12 h, and the cultivation time is 30 days.
[0048] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0049] Wherein, in step 1, the weight ratio of Gelsemium elegans seeds to tap water is 1:60.
[0050] Among them, the weight ratio of Gelsemium elegans seeds to detergent aqueous solution, alcohol and seed soaking agent is 1:20:20:20.
[0051] Among them, during the process of tap water soaking, disinfection treatment, and soaking in the seed soaking agent, a hard mesh plate with a pore size smaller than the particle size of the Gelsemium seeds is placed above the Gelsemium seeds to immerse the Gelsemium seeds in the liquid. In addition, during the soaking in the seed soaking agent, the Gelsemium seeds are placed in a sealed glass or stainless steel soaking container, and the upper end of the container is sealed and connected to the circulation pipeline through an air pump and an air pipe. The air pipe is evenly laid below the Gelsemium seeds (that is, the air pipe is evenly laid at the bottom of the seed soaking agent soaking container), and the air pipe is evenly provided with air holes. The mixed gas of ozone and negative hydrogen ions is circulated into the air pipe, and the mixed gas enters from the bottom of the Gelsemium seeds, flows to the top of the Gelsemium seeds, and then is collected from the upper end of the container and circulated and pumped in from the bottom of the Gelsemium seeds; wherein, the ozone concentration of the mixed gas initially introduced is 100 mg / L, and the average concentration of negative hydrogen ion gas is 2×10 6 / L, the mass volume ratio of Gelsemium elegans seeds to the mixed gas is 1g:10L, the mixed gas pumping flow rate is 0.05L / s, and the ventilation time is the same as the soaking time of the seed soaking agent. The mixed gas is obtained by mixing a set amount of ozone generated by an ozone generator and a set amount of negative hydrogen ions generated by a negative ion generator.
[0052] Comparative Example 1 Methods for germinating Gelsemium seeds include: Step 1: Place the air-dried Gelsemium seeds stored at room temperature for 3 months into a bottle and add tap water, and soak them in the tap water for 30 minutes; Step 2: The treated Gelsemium elegans seeds are sown in a germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32° C., the humidity of the incubator is 85%, the light intensity is 4000 lx, the light period is 12 h, the dark period is 12 h, and the cultivation time is 30 days.
[0053] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0054] Among them, the weight ratio of Gelsemium elegans seeds to tap water (soaking water) is 1:60.
[0055] Comparative Example 2 Methods for germinating Gelsemium seeds include: Step 1: Place the Gelsemium seeds that have been air-dried and stored at room temperature for 3 months into a bottle and add tap water. Soak the seeds in the tap water for 30 min. Then place the seeds and the soaked water in a 60°C water bath for 5 h, and then place them in a 4°C refrigerator for 5 h. Alternate between the water bath and the low temperature environment for 2 times. Step 2, filter out the Gelsemium seeds and bring them to room temperature for disinfection; wherein the specific steps of the disinfection are as follows: soak the Gelsemium seeds in a 0.1% detergent aqueous solution for 20 minutes, then soak them in 75% alcohol for 8 minutes, and then wash them with pure water for 3 times; Step 3: Sow the treated Gelsemium elegans seeds in the germination matrix and place them in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 32°C, the humidity of the incubator is 85%, the light intensity is 4000lx, the light period is 12h, the dark period is 12h, and the cultivation time is 30d.
[0056] The germination matrix includes a seedling matrix: vermiculite = 3:1; the amount of seeds per square meter is 3g. After sowing, a layer of 0.2mm thick fine seedling matrix is sprinkled, and then sprayed with a sprayer. The fine seedling matrix is sieved through a 900 mesh sieve. The seedling matrix is prepared by peat soil, perlite, and chicken manure in a ratio of 6:2:2.
[0057] Wherein, in step 1, the weight ratio of Gelsemium elegans seeds to tap water is 1:60.
[0058] Among them, the weight ratio of Gelsemium elegans seeds to detergent aqueous solution and alcohol is 1:20:20.
[0059] During the tap water soaking and disinfection process, a hard mesh plate with a pore size smaller than the Gelsemium elegans seed particle size is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
[0060] The methods of Examples 3 to 7 and Comparative Examples 1 to 2 were used to test the same batch of Gelsemium seeds that had been dried and stored at room temperature for 3 months. 300 Gelsemium seeds were randomly sown in each group and the test was carried out in the same constant temperature incubator and culture environment. The number of seeds that germinated and the growth were observed every day. The statistical results of Tables 1 and 2 are as follows: The number of germinations is calculated when the two cotyledons of the seeds unfold. The start of germination is the time when the two cotyledons of the first or more seeds unfold; the germination rate on day 18 = (the number of germinations counted on the 18th day after sowing / the total number of seeds) × 100%.
[0061] Table 1: Table 2: From the comparison of Table 1 and Table 2, we can see that: In Example 3, the stored Gelsemium elegans seeds were soaked in (sodium nitrophenolate + gibberellin) for 120 min, which can effectively improve the germination rate and promote early germination, which is significantly better than Comparative Examples 1 and 2. However, the uniform germination effect is not ideal. From the 8th day to the 18th day, the number of germinations changed significantly, affecting the uniform growth.
[0062] In Example 4, the stored Gelsemium elegans seeds were soaked in a seed soaking agent (sodium nitrophenolate + gibberellin + sodium bicarbonate) for 120 minutes, and the germination rate was better than that in Example 3. It can be inferred that by adding sodium bicarbonate to soak the seeds in combination with sodium nitrophenolate and gibberellin, the stability and activity of the seed soaking agent can be improved, and the surface and seed wings of the Gelsemium elegans seeds can be improved in water absorption and softened, thereby improving the cell activation effect.
[0063] In Example 5, the stored Gelsemium seeds were soaked in (compound sodium nitrophenolate + gibberellin) soaking agent for 50 min + temperature alternation. Under the condition of shortening the soaking time, the germination rate and the promotion of early germination were better than those of Example 3 and Example 4. It can be inferred that the alternating water bath temperature helps the Gelsemium seeds that are saturated with water to receive the water temperature more evenly, avoids the local overexcitation of the light Gelsemium seeds, and is conducive to the synchronous temperature alternation to promote the weakening of the tissues around the embryo. After the absorption and regulation of the sodium nitrophenolate and gibberellin soaking agent, the tissues around the embryo are further weakened, resulting in the rupture of the seed coat and the extension of the radicle, which promotes the awakening and germination of the seeds.
[0064] In Example 6, the stored Gelsemium seeds were soaked in a (compound sodium nitrophenolate + gibberellin + sodium bicarbonate) soaking agent for 50 min + temperature alternation. Under the condition of shortening the soaking time, the germination rate and the promotion of early germination were further better than those in Example 5. Figure 2 and Figure 3 As shown, by comparing the germination and growth conditions of Example 6 with those of Comparative Example 1, the germination rate, early germination effect and growth potential of Example 6 are significantly better than those of Comparative Example 1. It can be inferred that the sodium nitrophenolate, gibberellin and sodium bicarbonate components in the seed soaking agent not only have a synergistic effect, but also the seeds after the alternating water bath temperature treatment can also produce a synergistic effect with sodium nitrophenolate, gibberellin and sodium bicarbonate, which promotes the surface and seed wings of Gelsemium elegans seeds to improve water absorption and softening, and improves the cell activation effect.
[0065] In Example 7, the stored Gelsemium elegans seeds were soaked in a seed soaking agent of (sodium nitrophenolate + gibberellin + sodium bicarbonate) for 50 min + temperature alternation + mixed gas. When the soaking time was shortened, the germination rate and the effect of promoting early germination were more obvious, and the germination could be completed on the 5th and 6th days after sowing. Although the mixed gas was added during the soaking process, which increased the process and cost, it had a significant effect on promoting germination, early germination and neatness of the stored Gelsemium elegans seeds, which has a guiding significance for improving the efficiency of large-scale industrial production.
[0066] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and the embodiments. They can be applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized.
Claims
1. A method for promoting the germination of Gelsemium elegans seeds, characterized in that: include: The seeds of Gelsemium elegans are soaked in a seed soaking agent for 50-120 min, wherein the seed soaking agent contains 10-30 mg / L sodium nitrophenolate and 30-60 mg / L gibberellin, and the rest is pure water; The treated Gelsemium elegans seeds are sown in the germination matrix and placed in a constant temperature incubator for cultivation; during the cultivation period, the temperature is 20-32°C, the humidity of the incubator is 75-85%, the light intensity is 2000-4000lx, the light period is 12h, the dark period is 12h, and the cultivation time is 7-30d.
2. The method for promoting the germination of Gelsemium elegans seeds as claimed in claim 1, characterized in that: Before soaking with the seed soaking agent, it also includes: Step 1: Soak the air-dried Gelsemium seeds stored at room temperature in tap water for 20-30 min, then place the Gelsemium seeds and the soaking water in a 40-60℃ water bath for 3-5 h, and then place in a low temperature environment of 3-4℃ for 4-5 h, alternating between the water bath and the low temperature environment 2-3 times; Step 2: Filter out the Gelsemium elegans seeds, bring to room temperature, and disinfect them.
3. The method for promoting the germination of Gelsemium elegans seeds as claimed in claim 2, characterized in that: The Gelsemium seeds are air-dried Gelsemium seeds stored at room temperature for 2-4 months. The germination matrix comprises a seedling matrix: vermiculite=3:1; the sowing amount per square meter is 2-3g, and after sowing, a layer of 0.1-0.2mm thick fine seedling matrix is sprinkled and then sprayed wet, and the fine seedling matrix is sieved through an 800-900 mesh sieve.
4. The method for promoting the germination of Gelsemium elegans seeds as claimed in claim 3, characterized in that: In the step 1, the weight ratio of Gelsemium elegans seeds to tap water is 1:20-60.
5. The method for promoting the germination of Gelsemium elegans seeds as claimed in claim 4, characterized in that: The specific steps of the disinfection treatment are as follows: the Gelsemium elegans seeds are soaked in a 0.1% detergent aqueous solution for 20 minutes, then soaked in 75% alcohol for 8 minutes, and then washed with pure water for 3-4 times.
6. The method for promoting germination of Gelsemium elegans seeds as claimed in claim 5, characterized in that: The weight ratio of Gelsemium elegans seeds to the detergent aqueous solution, alcohol and the seed soaking agent is 1:10-20:10-20:10-20.
7. The method for promoting germination of Gelsemium elegans seeds as claimed in claim 6, characterized in that: During the process of tap water soaking, disinfection treatment and seed soaking agent soaking, a hard mesh plate with a hole diameter smaller than the particle diameter of the Gelsemium elegans seeds is placed above the Gelsemium elegans seeds to immerse the Gelsemium elegans seeds in the liquid.
8. The method for promoting the germination of Gelsemium elegans seeds according to any one of claims 1 to 7, characterized in that: The seed soaking agent also contains 50-80 mg / L of sodium bicarbonate.
9. The method for promoting the germination of Gelsemium elegans seeds as claimed in claim 8, characterized in that: During the soaking process of the seed soaking agent, air pipes are evenly laid under the Gelsemium seeds, and air holes are evenly arranged on the air pipes. A mixed gas of ozone and negative hydrogen ions is circulated into the air pipes. The mixed gas enters from the bottom of the Gelsemium seeds, flows to the top of the Gelsemium seeds, and is collected and circulated and pumped in from the bottom of the Gelsemium seeds. The ozone concentration of the mixed gas initially introduced is 50-100 mg / L, and the average concentration of the negative hydrogen ion gas is 1-2×10 6 / L, the mass volume ratio of Gelsemium elegans seeds to the mixed gas is 1g:5-10L, the mixed gas pumping flow rate is 0.01-0.05L / s, and the ventilation time is the same as the soaking time of the seed soaking agent.