Culture method for promoting seed germination and seedling growth of cercis chinensis

Abstract

The invention discloses a culture method for promoting germination of cercis chinensis seeds and growth of seedlings, which comprises the following steps: accelerating germination: soaking the cercis chinensis seeds in a seed soaking solution at normal temperature to treat for 3-4 hours, accelerating germination of the soaked cercis chinensis seeds for 5-9 days, and taking the germinated cercis chinensis seeds; sowing: dibbling the germinated cercis chinensis seeds in a sowing matrix, covering the seeds with 1-2cm of the sowing matrix, keeping the moisture of the sowing matrix at 50-65%, and separating cercis chinensis seedlings with 1-2 true leaves from the sowing matrix when the cercis chinensis seeds grow 1-2 true leaves; seedling cultivation: transplanting the 1-2 true-leaf cercis seedlings into a cultivation substrate, placing the cultivation substrate in a sunshade for cultivation, and applying a root inducing agent and a leaf fertilizer in the cultivation process to induce lateral roots of the cercis seedlings to grow until the height of the cercis seedlings is 50-60cm. Compared with the prior art, the method has the advantages that germination time is shortened by soaking seeds, lateral roots of the Chinese redbud seedlings are promoted to grow and extend by applying the culture medium, the root inducing agent and the leaf fertilizer, root weight and root system quantity are increased, and growth speed of the Chinese redbud seedlings is increased.

Description

Technical Field

[0001] The invention relates to the technical field of forest planting, and in particular to a cultivation method for promoting seed germination and seedling growth of Cercis chinensis. Background Art

[0002] Bauhinia (Madhucapasquieri), also known as wood peanut and star wood, is an evergreen tree of the genus Bauhinia in the family Sapotaceae. It is listed as a national key protected wild plant (Grade II). It is also a wild plant with extremely small populations, a national key plant protection object, and a key protected plant in the Guangxi Zhuang Autonomous Region.

[0003] At present, wild redbud trees are only distributed in small quantities in the southwestern part of Guangdong, the southern part of Guangxi Zhuang Autonomous Region, and the southeastern part of Yunnan. In order to protect the ecological resources of local redbud trees in Guangxi Zhuang Autonomous Region, the Guangxi Zhuang Autonomous Region Forestry Science Research Institute conducted basic research and multiple field surveys on redbud trees and found that the natural regeneration ability of redbud trees is poor. Through the collation, analysis and discussion of the survey data, the reasons for the poor natural regeneration ability of redbud trees are as follows:

[0004] First, the vitality period of redbud seeds is short. After the wild redbud fruits mature, they mostly fall on the dead branches and leaves under the forest, which hinders the contact between the redbud seeds and the soil. This makes it impossible for the redbud seeds to implant and germinate during the period of strong seed vitality, resulting in a low germination rate of wild redbud seeds.

[0005] Second, the growth rate of Bauhinia seedlings is slow, and the harsh environment in the wild makes it difficult for them to successfully survive the seedling stage and grow into young trees.

[0006] Therefore, Bauhinia wood needs artificial intervention in its germination and seedling cultivation to increase its germination rate and make it grow smoothly into saplings; Bin Yaomei and other colleagues root-cut the seedlings of Bauhinia wood from different sources after germination, retaining 2-4cm of the radicle, promoting the growth of the root system of Bauhinia wood and increasing its survival rate. However, root cutting leaves wounds, and incomplete disinfection of the incision will cause infection and death of the Bauhinia wood roots. Excessive disinfection will cause death of cells proximal to the incision, and the culture matrix is ​​difficult to disinfect thoroughly, resulting in microorganisms in the culture matrix infecting the Bauhinia wood seedlings through the wounds. These problems caused by root cutting reduce the survival rate of Bauhinia wood seedlings.

[0007] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0008] The purpose of the present invention is to provide a cultivation method for promoting the germination of seeds and the growth of seedlings of Bauhinia chinensis, aiming to overcome the problems of short vitality period of Bauhinia chinensis seeds, low germination rate, slow seedling growth rate and difficulty in surviving the seedling stage in the natural environment.

[0009] To achieve the above object, the present invention provides a cultivation method for promoting seed germination and seedling growth of Cercis chinensis, the method comprising:

[0010] S1, germination: taking Cercis chinensis seeds and immersing them in a seed soaking solution at room temperature for 3-4 hours, germinating the soaked Cercis chinensis seeds for 5-9 days, and taking the germinated Cercis chinensis seeds;

[0011] S2, sowing: sowing the germinated redbud seeds in the sowing matrix, then covering with 1-2 cm of the sowing matrix, and placing in a sunshade for cultivation, maintaining the moisture of the sowing matrix at 50-65%, and waiting for the redbud seeds to grow 1-2 true leaves, separating the redbud seedlings with 1-2 true leaves from the sowing matrix, and taking the redbud seedlings with 1-2 true leaves;

[0012] S3, seedling cultivation: transplanting the 1-2 true leaf Bauhinia chinensis seedlings into a cultivation medium, placing them in a sunshade for cultivation, applying root inducers and foliar fertilizers during the cultivation process to induce lateral root formation of the Bauhinia chinensis seedlings until the Bauhinia chinensis seedlings are 50-60 cm tall, wherein the root inducer comprises sodium nitrophenolate, sodium 5-nitroguaiacol, quercetin and La(NO 3 ) 3 The components of the foliar fertilizer include urea, glucose, naphthylacetic acid and sodium 5-nitroguaiacol.

[0013] Preferably, in the above technical solution, in step S1, the specific steps of accelerating germination include:

[0014] S11, taking Cercis chinensis seeds and immersing them in a soaking solution at room temperature for 3-4 hours to obtain Cercis chinensis seeds;

[0015] S12, spreading the tray with fabric soaked in soaking liquid, placing the soaked redbud seeds on the fabric, and covering with a layer of fabric moistened with water;

[0016] S13, placing the tray in a germination box for germination treatment for 5-7 days, spraying the seed soaking solution daily to keep the fabric moist during the germination treatment, and setting the germination conditions of the germination box to include light intensity of 800-1000Lx, light time of 4-6h, and temperature of 25-33°C.

[0017] Preferably, in the above technical solution, in step S1, the seed soaking agent is naphthylacetic acid or gibberellin.

[0018] Preferably, in the above technical solution, the seed soaking agent is 0.1-1 mg / L naphthaleneacetic acid or 0.1-1.4 mg / L gibberellin.

[0019] According to the above technical scheme, 0.1-1 mg / L naphthaleneacetic acid or 0.1-1.4 mg / L gibberellin is used as the seed soaking solution. Naphthaleneacetic acid or gibberellin enters into the Bauhinia seeds by osmosis during the seed soaking process, promotes starch conversion and energy release in the Bauhinia seeds, and provides the required large amount of energy and nutrition for the increase of cell metabolism rate when the radicle of the Bauhinia seeds is formed.

[0020] Preferably, in the above technical solution, the root inducer is composed of 1-5% sodium nitrophenolate, 3-10% sodium 5-nitroguaiacol, 5-10% quercetin, 1-5% La(NO 3 ) 3 and 70-90% water.

[0021] Preferably, in the above technical solution, the components of the foliar fertilizer are, by percentage, 45-80% water, 20-30% urea, 1-5% glucose, 5-10% naphthaleneacetic acid and 5-10% sodium 5-nitroguaiacol.

[0022] According to the above technical scheme, sodium nitrophenolate can promote the penetration of root inducer from the root surface into the cells of the root system, and sodium nitrophenolate, sodium 5-nitroguaiacol and La(NO 3 ) 3 Together, they promote the formation of lateral root progenitors, increase the division and elongation of lateral root progenitor cells, and promote the increase in the number and length of lateral roots; and quercetin enhances the transport of auxin and improves root growth. Adding sodium 5-nitroguaiacol to foliar fertilizers enhances the penetration of foliar fertilizers and promotes the absorption of foliar fertilizers by leaves.

[0023] Preferably, in the above technical solution, the sowing matrix is ​​a mixture of soil, straw and wood ash in a mass ratio of 5-10:2-6:1-2.

[0024] Preferably, in the above technical solution, the cultivation matrix is ​​soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate in a mass ratio of 5-10:2-6:1-2:1-2:0.5-1:1-2.

[0025] Preferably, in the above technical solution, in the seedling cultivation step, the inducer is applied once every 5-7 days during the seedling cultivation process and foliar fertilizer is sprayed on the true leaves.

[0026] Compared with the prior art, the present invention has the following beneficial technical effects:

[0027] (1) The use of low concentrations of gibberellins or naphthylacetic acid can promote the rapid conversion of starch in the seeds of Cercis chinensis into soluble sugars and other substances to provide energy for the germination of the seeds of Cercis chinensis, shorten the time for the seeds of Cercis chinensis to germinate and form radicles, thereby shortening the germination time and increasing the germination rate;

[0028] (2) Calcium nitrate, boric acid and potassium nitrate were added to the seedling culture medium, calcium ions, low concentration nitrate ions, sodium nitrophenolate, sodium 5-nitroguaiacol, La(NO 3 ) 3 It works synergistically with foliar fertilizers to increase root weight and the number of lateral roots. Potassium ions promote the absorption of nutrients by the roots, increasing the number of leaves, making the ground diameter thicker, and allowing seedlings to grow faster. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings.

[0030] Figure 1 It is a graph of the germination rate of Cercis chinensis seeds treated with 0.1 mg / L, 1 mg / L, 10 mg / L, and 50 mg / L naphthaleneacetic acid of the present invention;

[0031] Figure 2 It is a graph of the germination rate of Cercis chinensis seeds treated with 0.2 mg / L, 0.4 mg / L, 0.6 mg / L, and 0.8 mg / L naphthaleneacetic acid of the present invention;

[0032] Figure 3 It is a graph of the germination rate of Cercis chinensis seeds treated with gibberellins at concentrations of 0.1 mg / L, 1 mg / L, 10 mg / L, and 50 mg / L of the present invention;

[0033] Figure 4 It is a graph of the germination rate of Cercis chinensis seeds treated with gibberellins at concentrations of 0.6 mg / L, 0.8 mg / L, 1.2 mg / L, and 1.6 mg / L of the present invention;

[0034] Figure 5 is a diagram of a Cercis chinensis seedling with 1-2 true leaves in a specific embodiment of the present invention;

[0035] Figure 6 This is a picture of a Cercis australis seedling after 6 months of seedling cultivation in a specific embodiment of the present invention. DETAILED DESCRIPTION

[0036] Example 1

[0037] Different concentrations of naphthaleneacetic acid were used to treat the seeds of Cercis chinensis:

[0038] When the redbud trees matured in April and May, 240 full redbud seeds were randomly selected and immersed in 1000mL naphthaleneacetic acid (with concentrations of 0.1mg / L, 1mg / L, 10mg / L, and 50mg / L) for 4 hours at 25-30℃. They were then placed in a germination incubator for germination treatment. Three replicate test groups were set for each concentration, with 20 redbud seeds in each group. The germination results are shown in the figure. Figure 1As shown in the figure, when the seeds of Cercis chinensis were treated with 10 mg / L naphthaleneacetic acid and 1 mg / L naphthaleneacetic acid, the germination rate of Cercis chinensis seeds decreased with the increase of naphthaleneacetic acid concentration, indicating that the germination rate of Cercis chinensis seeds soaked in 0.1-1 mg / L naphthaleneacetic acid was higher than that soaked in 10 mg / L and 50 mg / L, indicating that 0.1-1 mg / L naphthaleneacetic acid can promote the germination of Cercis chinensis seeds.

[0039] Take 240 full seeds of Bauhinia chinensis, soak them in 1000mL of naphthaleneacetic acid (concentrations of 0.2mg / L, 0.4mg / L, 0.6mg / L, and 0.8mg / L) at 25-30℃ for 4h, and place them in a germination incubator for germination treatment. The germination results are as follows Figure 2 And as shown in Table 1.

[0040] Table 1 Seed germination rate of Cercis chinensis seeds treated with different concentrations of naphthaleneacetic acid

[0041]

[0042]

[0043] from Figure 2 As can be seen from Table 1, treating the seeds of Cercis chinensis with 0.1-1 mg / L naphthaleneacetic acid can increase its germination rate. At the same time, treating the seeds of Cercis chinensis with 0.6-1 mg / L naphthaleneacetic acid can basically complete germination on the 6th day, and all the seeds of Cercis chinensis that can germinate can germinate on the 7th day.

[0044] Example 2

[0045] Different concentrations of gibberellins were used to treat the seeds of Bauhinia chinensis:

[0046] When the redbud tree matured in April and May, 240 full redbud tree seeds were randomly selected and immersed in 1000mL gibberellin (concentrations of 0.1mg / L, 1mg / L, 10mg / L, and 50mg / L) for 4 hours at 25-30℃. They were then placed in a germination incubator for germination treatment. Three replicate test groups were set for each concentration, with 20 redbud tree seeds in each group. The results are as follows Figure 3 As shown, the seeds of Cercis chinensis are sensitive to the concentration of gibberellins, which can promote the germination of Cercis chinensis seeds and shorten the germination time within a certain range.

[0047] To further explore the effect of 0.1-1.4 mg / L gibberellin on the treatment of Bauhinia seeds, 240 full Bauhinia seeds were randomly selected and immersed in 1000 mL of gibberellin (at concentrations of 0.6 mg / L, 0.8 mg / L, 1.2 mg / L, and 1.4 mg / L) for 4 h at 25-30 °C. They were then placed in a germination incubator for germination treatment. Three replicate test groups were set for each concentration, with 20 Bauhinia seeds in each group. The results are as follows: Figure 4 As shown in Figure 2, the germination of Cercis chinensis seeds treated with 0.1 mg / L, 0.6 mg / L, 0.8 mg / L, 1 mg / L, 1.2 mg / L, and 1.4 mg / L gibberellins at 4-9 days is shown in Figure 2. Figure 4 And as shown in Table 2.

[0048] Table 2 Germination rate of Cercis chinensis seeds treated with different concentrations of gibberellins

[0049] concentration 4d 5d 6d 7d 8d 9d 0.1mg / L 3.33％ 33.33％ 78.33％ 90.00％ 98.33％ 98.33％ 0.6mg / L 8.33％ 63.33％ 90.00％ 98.33％ 98.33％ 98.33％ 0.8mg / L 13.33％ 70.00％ 95.00％ 98.33％ 98.33％ 100.00％ 1mg / L 16.67％ 75.00％ 100.00％ 100.00％ 100.00％ 100.00％ 1.2mg / L 16.67％ 76.67％ 98.33％ 98.33％ 98.33％ 98.33％ 1.4mg / L 16.67％ 78.33％ 100.00％ 100.00％ 100.00％ 100.00％

[0050] from Figure 4 As can be seen from Table 2, 0.1-1.4 mg / L gibberellin can shorten the germination time of Cercis chinensis, especially 1-1.4 mg / L gibberellin, which has completed most of the germination on the 6th day after treatment.

[0051] 180 full seeds of Bauhinia chinensis were taken as test objects. At 25-30℃, 60 full seeds were respectively placed in 1000mL1.2mg / L gibberellin, 1mg / L naphthaleneacetic acid and clean water for 4h, and then placed in a germination incubator for germination treatment. Three seeds were randomly selected on the 0th to 9th day of germination to detect the changes in the starch content in the seeds. The results are shown in Table 3.

[0052] Table 3 Changes in starch content (mg / g) in Cercis chinensis seeds after treatment with water, naphthaleneacetic acid and gibberellins

[0053] Group 0d 1d 2d 3d 4d 5d 6d 7d 8d 9d Shimizu 0.57 0.56 0.53 0.49 0.45 0.36 0.31 0.25 0.23 0.21 Gibberellins 0.55 0.51 0.46 0.40 0.36 0.31 0.25 0.23 0.22 0.21 Naphthaleneacetic acid 0.56 0.53 0.48 0.43 0.38 0.29 0.23 0.21 0.20 0.21

[0054] As can be seen from Table 3, with the increase of germination time, the average starch content of Bauhinia seeds showed a downward trend and tended to stabilize after a certain period of time, indicating that the starch content of Bauhinia seeds decreased before germination of Bauhinia seeds, which taught that starch was converted into other substances during the germination of Bauhinia seeds to promote the germination of Bauhinia seeds; the starch content in Bauhinia seeds in the gibberellin group and the naphthylacetic acid group decreased faster, indicating that gibberellin and naphthylacetic acid promoted the conversion of starch in Bauhinia seeds into other substances, thereby providing energy for radicle growth.

[0055] Example 3

[0056] A cultivation method for promoting seed germination and seedling growth of Cercis chinensis, the method comprising:

[0057] S1, germination, the specific steps are as follows:

[0058] S11, taking 30 full seeds of Cercis chinensis and immersing them in 1000 mL of 1 mg / L gibberellin solution at room temperature for 4 hours to obtain Cercis chinensis seeds;

[0059] S12, spreading the tray with fabric soaked in soaking liquid, placing the soaked seeds of Bauhinia chinensis on the fabric without overlapping adjacent seeds, and covering with a layer of fabric moistened with water to prevent water volatilization of the seeds during the germination process from affecting the germination of the Bauhinia chinensis seeds;

[0060] S13, placing the tray in a germination box with a light intensity of 800 Lx, a light duration of 4 h, and a temperature of 25° C. for germination treatment for 7 days, spraying a seed soaking solution daily during the germination treatment to keep the fabric moist until the seeds of the redbud tree germinate;

[0061] S2, sowing, the specific steps are as follows:

[0062] S21, weighing soil, straw, and plant ash respectively in a mass ratio of 5:2:1, and mixing the weighed soil, straw, and plant ash evenly to prepare a sowing medium;

[0063] S22, sowing the germinated seeds in a culture bag filled with a sowing matrix, then covering with 1-2 cm of matrix and gently pressing, placing in a sunshade for cultivation, maintaining the moisture content of the sowing matrix at 50-65% during the cultivation process, and applying water to the roots when the redbud tree grows to 1-2 true leaves, separating the 1-2 true leaf seedlings from the sowing matrix after the soil softens, and taking the 1-2 true leaf redbud tree seedlings;

[0064] S3, seedling cultivation, the specific steps are as follows:

[0065] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 5:2:1:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0066] S32, transplanting the 1-2 true-leaf Bauhinia chinensis seedling into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Bauhinia chinensis seedling with the culture medium, soaking the culture medium with a root inducer, applying the inducer once every 5 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia chinensis seedling, and treating until the Bauhinia chinensis seedling is 50-60 cm tall, wherein the root inducer is composed of 5% sodium nitrophenolate, 10% sodium 5-nitroguaiacol, 5% quercetin, 5% La(NO 3 ) 3and 75% water, and the components of the foliar fertilizer are 67% water, 20% urea, 1% glucose, 7% naphthylacetic acid and 5% sodium 5-nitroguaiacol.

[0067] Example 4

[0068] A cultivation method for promoting seed germination and seedling growth of Cercis chinensis, the method comprising:

[0069] S1, germination, the specific steps are as follows:

[0070] S11, taking 30 full seeds of Cercis chinensis and immersing them in 1000 mL of 0.8 mg / L gibberellin solution at room temperature for 4 hours to obtain Cercis chinensis seeds;

[0071] S12, spreading the tray with fabric soaked in soaking liquid, placing the soaked seeds of Bauhinia chinensis on the fabric without overlapping adjacent seeds, and covering with a layer of fabric moistened with water to prevent water volatilization of the seeds during the germination process from affecting the germination of the Bauhinia chinensis seeds;

[0072] S13, placing the tray in a germination box with a light intensity of 900, a light duration of 5 hours, and a temperature of 30° C. for germination treatment for 7 days, spraying a seed soaking solution daily during the germination treatment to keep the fabric moist until the seeds of the redbud tree germinate;

[0073] S2, sowing, the specific steps are as follows:

[0074] S21, weighing soil, straw, and plant ash respectively in a mass ratio of 9:6:2, and mixing the weighed soil, straw, and plant ash evenly to prepare a sowing medium;

[0075] S22, sowing the germinated seeds in a culture bag filled with a sowing matrix, then covering with 1-2 cm of matrix and gently pressing, placing in a sunshade for cultivation, maintaining the moisture content of the sowing matrix at 50-65% during the cultivation process, and applying water to the roots when the redbud tree grows to 1-2 true leaves, separating the 1-2 true leaf seedlings from the sowing matrix after the soil softens, and taking the 1-2 true leaf redbud tree seedlings;

[0076] S3, seedling cultivation, the specific steps are as follows:

[0077] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 9:6:2:2:1:2, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0078] S32, transplanting the 1-2 true-leaf Bauhinia spp. seedling into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Bauhinia spp. seedling with the culture medium, soaking the culture medium with a root inducer, applying the inducer once every 6 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia spp. seedling, and treating until the Bauhinia spp. seedling is 50-60 cm tall, wherein the root inducer is composed of 3% sodium nitrophenolate, 6% sodium 5-nitroguaiacol, 8% quercetin, 4% La(NO 3 ) 3 and 79% water, and the components of the foliar fertilizer are 55% water, 20% urea, 5% glucose, 10% naphthylacetic acid and 10% sodium 5-nitroguaiacol.

[0079] Example 5

[0080] A cultivation method for promoting seed germination and seedling growth of Cercis chinensis, the method comprising:

[0081] S1, germination, the specific steps are as follows:

[0082] S11, taking 30 full seeds of Cercis chinensis and immersing them in 1000 mL of 1.2 mg / L naphthaleneacetic acid solution at room temperature for 4 hours to obtain Cercis chinensis seeds;

[0083] S12, spreading the tray with fabric soaked in soaking liquid, placing the soaked seeds of Bauhinia chinensis on the fabric without overlapping adjacent seeds, and covering with a layer of fabric moistened with water to prevent water volatilization of the seeds during the germination process from affecting the germination of the Bauhinia chinensis seeds;

[0084] S13, placing the tray in a germination box with a light intensity of 1000 Lx, a light duration of 6 h, and a temperature of 33° C. for germination treatment for 7 days, spraying a seed soaking solution daily during the germination treatment to keep the fabric moist until the seeds of the redbud tree germinate;

[0085] S2, sowing, the specific steps are as follows:

[0086] S21, weighing soil, straw, and plant ash respectively in a mass ratio of 6:3:2, and mixing the weighed soil, straw, and plant ash evenly to prepare a sowing matrix;

[0087] S22, sowing the germinated seeds in a culture bag filled with a sowing matrix, then covering with 1-2 cm of matrix and gently pressing, placing in a sunshade for cultivation, maintaining the moisture content of the sowing matrix at 50-65% during the cultivation process, and applying water to the roots when the redbud tree grows to 1-2 true leaves, separating the 1-2 true leaf seedlings from the sowing matrix after the soil softens, and taking the 1-2 true leaf redbud tree seedlings;

[0088] S3, seedling cultivation, the specific steps are as follows:

[0089] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 6:3:2:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a culture matrix, and filling the bottom of the culture bag with the culture matrix;

[0090] S32, transplanting the 1-2 true-leaf Bauhinia spp. seedling into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Bauhinia spp. seedling with the culture medium, soaking the culture medium with a root inducer, applying the inducer once every 5-7 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia spp. seedling, and treating until the Bauhinia spp. seedling is 50-60 cm tall, wherein the root inducer is composed of 5% sodium nitrophenolate, 10% sodium 5-nitroguaiacol, 5% quercetin, 5% La(NO 3 ) 3 and 75% water, and the components of the foliar fertilizer are 67% water, 20% urea, 1% glucose, 7% naphthylacetic acid and 5% sodium 5-nitroguaiacol.

[0091] Example 6

[0092] A cultivation method for promoting seed germination and seedling growth of Cercis chinensis, the method comprising:

[0093] S1, germination, the specific steps are as follows:

[0094] S11, taking 30 full seeds of Cercis chinensis and immersing them in 1000 mL of 1.4 mg / L naphthaleneacetic acid solution at room temperature for 4 hours to obtain Cercis chinensis seeds;

[0095] S12, spreading the tray with fabric soaked in soaking liquid, placing the soaked seeds of Bauhinia chinensis on the fabric without overlapping adjacent seeds, and covering with a layer of fabric moistened with water to prevent water volatilization of the seeds during the germination process from affecting the germination of the Bauhinia chinensis seeds;

[0096] S13, placing the tray in a germination box with a light intensity of 800 Lx, a light duration of 6 h, and a temperature of 25° C. for germination treatment for 5-9 days, spraying a seed soaking solution daily during the germination treatment to keep the fabric moist until the seeds of the redbud tree germinate;

[0097] S2, sowing, the specific steps are as follows:

[0098] S21, weighing soil, straw, and plant ash respectively in a mass ratio of 9:6:2, and mixing the weighed soil, straw, and plant ash evenly to prepare a sowing medium;

[0099] S22, sowing the germinated seeds in a culture bag filled with a sowing matrix, then covering with 1-2 cm of matrix and gently pressing, placing in a sunshade for cultivation, maintaining the moisture content of the sowing matrix at 50-65% during the cultivation process, and applying water to the roots when the redbud tree grows to 1-2 true leaves, separating the 1-2 true leaf seedlings from the sowing matrix after the soil softens, and taking the 1-2 true leaf redbud tree seedlings;

[0100] S3, seedling cultivation, the specific steps are as follows:

[0101] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 9:6:2:2:1:2, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0102] S32, transplanting the 1-2 true-leaf Bauhinia spp. seedling into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Bauhinia spp. seedling with the culture medium, soaking the culture medium with a root inducer, applying the inducer once every 5-7 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia spp. seedling, and treating until the Bauhinia spp. seedling is 50-60 cm tall, wherein the root inducer is composed of 1% sodium nitrophenolate, 3% sodium 5-nitroguaiacol, 5% quercetin, 1% La(NO 3 ) 3 and 90% water, and the components of the foliar fertilizer are 55% water, 20% urea, 5% glucose, 10% naphthylacetic acid and 10% sodium 5-nitroguaiacol.

[0103] Comparative Example 1

[0104] It is basically the same as Example 3, except that the seedling cultivation method is different.

[0105] S3, seedling cultivation, the specific steps are as follows:

[0106] S31, weighing soil, straw, and plant ash respectively in a mass ratio of 5:2:1, mixing the weighed soil, straw, and plant ash evenly to prepare a culture matrix, and filling the bottom of the culture bag with the culture matrix;

[0107] S32, transplanting the 1-2 true-leaf Cercis chinensis seedlings into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Cercis chinensis seedlings with the culture medium, soaking the culture medium with clean water, and applying clean water once every 5 days during the seedling cultivation process.

[0108] Comparative Example 2

[0109] It is basically the same as Example 3, except that the seedling cultivation method is different.

[0110] S3, seedling cultivation, the specific steps are as follows:

[0111] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 5:2:1:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0112] S32, transplanting the 1-2 true-leaf Bauhinia chinensis seedling into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Bauhinia chinensis seedling with the culture medium, soaking the culture medium with clean water, and applying clean water once every 5 days during the seedling cultivation process to promote the root growth of the Bauhinia chinensis seedling.

[0113] Comparative Example 3

[0114] It is basically the same as Example 3, except that the seedling cultivation method is different.

[0115] S3, seedling cultivation, the specific steps are as follows:

[0116] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 5:2:1:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0117] S32, transplanting the 1-2 true-leaf Bauhinia chinensis seedlings into a culture bag filled with a culture matrix, filling the area around the 1-2 true-leaf Bauhinia chinensis seedlings with the culture matrix, infiltrating the culture matrix with a root inducer, applying the inducer once every 5 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia chinensis seedlings, wherein the components of the root inducer are 5% quercetin, 10% sodium 5-nitroguaiacol and 85% water, and the components of the foliar fertilizer are 67% water, 20% urea, 1% glucose, 7% naphthylacetic acid and 5% sodium 5-nitroguaiacol.

[0118] Comparative Example 4

[0119] It is basically the same as Example 3, except that the seedling cultivation method is different.

[0120] S3, seedling cultivation, the specific steps are as follows:

[0121] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 5:2:1:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0122] S32, transplanting the 1-2 true-leaf Bauhinia spp. seedlings into a culture bag filled with a culture matrix, filling the area around the 1-2 true-leaf Bauhinia spp. seedlings with the culture matrix, infiltrating the culture matrix with a root inducer, applying the inducer once every 5 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia spp. seedlings, wherein the components of the root inducer are 5% sodium nitrophenolate, 5% quercetin, 10% sodium 5-nitroguaiacol and 80% water, and the components of the foliar fertilizer are 67% water, 20% urea, 1% glucose and 7% naphthaleneacetic acid.

[0123] Comparative Example 5

[0124] It is basically the same as Example 3, except that the seedling cultivation method is different.

[0125] S3, seedling cultivation, the specific steps are as follows:

[0126] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 5:2:1:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0127] S32, transplanting the 1-2 true-leaf Bauhinia spp. seedling into a culture bag filled with a culture matrix, filling the area around the 1-2 true-leaf Bauhinia spp. seedling with the culture matrix, infiltrating the culture matrix with a root inducer, applying the inducer once every 5 days during the seedling cultivation process, and spraying foliar fertilizer on the true leaves to promote the root growth of the Bauhinia spp. seedling, wherein the components of the root inducer are 5% sodium nitrophenolate, 10% sodium 5-nitroguaiacol and 90% water, and the components of the foliar fertilizer are 85% water, 5% glucose and 10% naphthaleneacetic acid.

[0128] Comparative Example 6

[0129] It is basically the same as Example 3, except that the seedling cultivation method is different.

[0130] S3, seedling cultivation, the specific steps are as follows:

[0131] S31, weighing soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate respectively in a mass ratio of 5:2:1:1:0.5:1, mixing the weighed soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate evenly to prepare a cultivation matrix, and filling the bottom of the culture bag with the cultivation matrix;

[0132] S32, transplanting the 1-2 true-leaf Bauhinia chinensis seedlings into a culture bag filled with a culture medium, filling the area around the 1-2 true-leaf Bauhinia chinensis seedlings with the culture medium, infiltrating the culture medium with a root inducer, applying the inducer once every 5 days during the seedling cultivation process to promote the root growth of the Bauhinia chinensis seedlings, wherein the root inducer comprises 5% sodium nitrophenolate, 10% sodium 5-nitroguaiacol, 5% quercetin, 5% La(NO 3 ) 3 and 75% water.

[0133] According to Examples 3-6 and Comparative Examples 1-6, seeds of Bauhinia trees were selected for germination and sowing. In each example, 20 Bauhinia tree seedlings with 1-2 true leaves were selected in May 2021 for seedling cultivation. The seedling height of the Bauhinia tree seedlings was measured on November 2021, May 2022, November 2022, and January 1-5, 2023, respectively. The results are shown in Table 4.

[0134] Table 4 Growth of seedlings in each group detected at fixed time

[0135]

[0136]

[0137] It can be seen from Table 4 that Examples 3-4 using the method of the present invention have a faster growth rate, and the increasing rate is greater than that of Comparative Examples 1-6, especially greater than that of the Cercis chinensis seedlings treated with Comparative Examples 1-2.

[0138] When the seedlings of Cercis chinensis were 50-60 cm tall, 4 plants were randomly selected from each group to measure the root weight, number of lateral roots, number of leaves, ground diameter and survival rate. The measurement time was recorded. The results are shown in Table 5.

[0139] Table 5 Nutritional growth of Cercis chinensis seedlings when they are 50-60 cm high in Examples 3-6 and Comparative Examples 1-6

[0140] Measurement time Root weight / g Number of lateral roots / root Number of leaves / pieces Ground diameter / mm Example 3 November 2022 2.67 25.5 36 8.62 Example 4 November 2022 2.63 24.5 36 8.57 Example 5 November 2022 2.65 25.0 35 8.61 Example 6 November 2022 2.61 24.0 34 8.45 Comparative Example 1 May 2024 1.74 9.5 28 6.46 Comparative Example 2 February 2024 2.04 13.0 26 7.25 Comparative Example 3 June 2023 2.13 15.5 32 8.16 Comparative Example 4 March 2023 2.44 21.0 28 7.98 Comparative Example 5 April 2023 2.29 18.5 30 8.26 Comparative Example 6 March 2023 2.51 22.5 35 8.34

[0141] From Table 5, it can be seen that the seedlings of Bauhinia chinensis in Examples 3-6 grow faster, and the seedlings can grow to 50-60 cm in 15 months, and the number of lateral roots is significantly more than that of Comparative Examples 1-6. Compared with Comparative Example 2, the root weight and the number of lateral roots of Comparative Example 1 increase, and the growth rate is faster, indicating that the application of calcium nitrate, boric acid and potassium nitrate in the matrix improves the root growth and seedling growth of Bauhinia chinensis seedlings. The principle may be that calcium ions and low concentrations of nitrate ions promote the formation and elongation of lateral roots, and potassium ions promote the lateral roots to absorb other nutrients in the culture matrix, thereby improving the growth rate of Bauhinia chinensis seedlings; Compared with Example 3, the application of foliar fertilizer to the leaves of Comparative Examples 5-6 can supplement nitrogen fertilizer to promote faster growth of Bauhinia chinensis seedlings.

[0142] 50-60 cm of each group were transplanted to the natural environment, and the survival of the Cercis australis seedlings was recorded one year after transplantation. The results are shown in Table 6.

[0143] Table 6 Survival of Cercis chinensis seeds after transplantation at a height of 50-60 cm in Examples 3-6 and Comparative Examples 1-6

[0144] Group Survival rate Example 3 92.33 Example 4 93.67 Example 5 92.67 Example 6 93.67 Comparative Example 1 56.50 Comparative Example 2 73.33 Comparative Example 3 82.50 Comparative Example 4 88.33 Comparative Example 5 87.50 Comparative Example 6 91.33

[0145] The present invention can be implemented in various ways and is not limited to the embodiments described. A person skilled in the art can understand that the present invention can be implemented in other specific ways without changing the technical concept or essential features of the present invention. Therefore, it should be understood that the embodiments described above are exemplary and are not intended to limit the present invention.

Claims

1. A method for promoting seed germination and seedling growth of Cercis chinensis, characterized in that: The method comprises: S1, germination: taking Cercis chinensis seeds and immersing them in a seed soaking solution at room temperature for 3-4 hours, germinating the soaked Cercis chinensis seeds for 5-9 days, and taking the germinated Cercis chinensis seeds; S2, sowing: sowing the germinated redbud seeds in the sowing matrix, then covering with 1-2 cm of the sowing matrix, and placing in a sunshade for cultivation, maintaining the moisture of the sowing matrix at 50-65%, and waiting for the redbud seeds to grow 1-2 true leaves, separating the redbud seedlings with 1-2 true leaves from the sowing matrix, and taking the redbud seedlings with 1-2 true leaves; S3, seedling cultivation: transplant the 1-2 true leaf Bauhinia seedlings into a cultivation medium, place them in a sunshade for cultivation, and apply root inducers and foliar fertilizers during the cultivation process to induce lateral root formation of the Bauhinia seedlings until the Bauhinia seedlings are 50-60 cm tall, wherein the components of the root inducer include sodium nitrophenolate, sodium 5-nitroguaiacol, quercetin and La(NO3)3, and the components of the foliar fertilizer include urea, glucose, naphthylacetic acid and sodium 5-nitroguaiacol.

2. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 1, characterized in that: In step S1, the specific steps of accelerating germination include: S11, taking Cercis chinensis seeds and immersing them in a soaking solution at room temperature for 3-4 hours to obtain Cercis chinensis seeds; S12, spreading the tray with fabric soaked in soaking liquid, placing the soaked redbud seeds on the fabric, and covering with a layer of fabric moistened with water; S13, placing the tray in a germination box for germination treatment for 5-7 days, spraying the seed soaking solution daily to keep the fabric moist during the germination treatment, and setting the germination conditions of the germination box to include light intensity of 800-1000Lx, light time of 4-6h, and temperature of 25-33°C.

3. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 2, characterized in that: In step S1, the seed soaking agent is naphthylacetic acid or gibberellin.

4. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 3, characterized in that: The seed soaking agent is 0.1-1 mg / L naphthaleneacetic acid or 0.1-1.4 mg / L gibberellin.

5. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 1, characterized in that: Calculated by percentage, the root inducer comprises 1-5% sodium nitrophenolate, 3-10% sodium 5-nitroguaiacol, 5-10% quercetin, 1-5% La(NO3)3 and 70-90% water.

6. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 1, characterized in that: In terms of percentage, the components of the foliar fertilizer are 45-80% water, 20-30% urea, 1-5% glucose, 5-10% naphthaleneacetic acid and 5-10% sodium 5-nitroguaiacol.

7. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 1, characterized in that: The sowing matrix is ​​a mixture of soil, straw and wood ash in a mass ratio of 5-10:2-6:1-2.

8. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 1, characterized in that: The cultivation matrix is ​​soil, straw, wood ash, calcium nitrate, boric acid and potassium nitrate in a mass ratio of 5-10:2-6:1-2:1-2:0.5-1:1-2.

9. The method for promoting seed germination and seedling growth of Cercis chinensis according to claim 1, characterized in that: In the seedling cultivation step, the inducer is applied once every 5-7 days during the seedling cultivation process and foliar fertilizer is sprayed on the true leaves.

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