Macrobrachium rosenbergii parent shrimp catching and transporting method

Abstract

The invention relates to the technical field of aquaculture, in particular to a catching and transporting method for macrobrachium rosenbergii parent shrimps. Comprising the following steps: (1) etiology detection: detecting main pathogens of macrobrachium rosenbergii by adopting a real-time fluorescent PCR technology; (2) preparation before fishing: adding a shrimp shell hardening accelerant into the culture pond before fishing; (3) catching: adopting a mode of combining net-pulling scraping catching with dry-pond catching; (4) net cage temporary culture: adding a shrimp shell hardening accelerant and an anti-stress plant extract into a temporary culture water body to relieve stress reaction; (5) carrying out transportation with water, adding an anti-stress plant extract into a transportation water body, and monitoring transportation environment parameters in real time; and (6) treatment after transportation: carrying out temporary rearing and innocent treatment on the seed shrimps arriving at the destination. According to the method, through application of the shrimp shell hardening accelerant and the anti-stress plant extract and whole-process water quality and environment monitoring, the survival rate and the health state of the breeding shrimps are remarkably improved, and the method is suitable for fishing, temporary rearing and transportation of large-scale aquaculture breeding shrimps.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a method for catching and transporting Macrobrachium rosenbergii seedlings. In particular, the method significantly improves the survival rate and health status of the seedlings by adding a shrimp shell hardening accelerator before catching and in the temporary rearing stage and adding an anti-stress plant extract in the temporary rearing and transportation stages. The method is suitable for the catching, temporary rearing and transportation of seedlings in large-scale aquaculture. Background Art

[0002] Macrobrachium rosenbergii Macrobrachium rosenbergii ) is a freshwater aquaculture species with high economic value. It is widely cultivated because of its delicious meat, strong adaptability and fast growth rate. In the process of aquaculture, the quality and survival rate of seed shrimp directly determine the success or failure of subsequent breeding and seedling cultivation. Conventionally farmed commercial shrimp generally enter the catering consumption market immediately through the circulation link, while seed shrimp enter the nursery after being caught and transported. They need to continue to be kept alive and intensively cultivated before being used to reproduce offspring. The technical requirements for fishing and transportation are more stringent than those for commercial shrimp. Therefore, the standardization of operations and technical improvements in the process of catching and transporting seed shrimp are of great significance to improving the quality and survival rate of seed shrimp.

[0003] In the prior art, the fishing and transportation of Macrobrachium rosenbergii broodstock have the following main problems: 1. Risk of disease transmission: Failure to conduct adequate pathogen testing before fishing may result in shrimps carrying pathogens entering the transportation process, increasing the possibility of disease spread; 2. Severe stress response: During the fishing and transportation process, the shrimps will experience strong stress response due to high density, insufficient dissolved oxygen or drastic changes in the environment (such as large temperature differences), resulting in a decrease in survival rate; 3. Insufficient hardening of shrimp shells: The shells of seed shrimps are not hardened sufficiently before and after fishing or there are many soft-shell shrimps, which makes them susceptible to loss during fishing and transportation, becoming one of the most important factors affecting the survival rate of seed shrimps; 4. Imperfect water quality management: Inadequate water quality maintenance measures in transportation and temporary water bodies, such as inadequate control of dissolved oxygen, ammonia nitrogen, nitrite and disinfection treatment, further reducing the survival ability of broodstock shrimp; 5. Lack of monitoring and record keeping: During the fishing and transportation process, environmental parameters are not monitored in real time, making it difficult to adjust operations in a timely manner according to emergencies. Any carelessness can cause significant economic losses.

[0004] To solve the above problems, some technologies have proposed improvement plans for the capture and transportation of Macrobrachium rosenbergii broodstock, such as using high-oxygen saturated water and optimizing transportation density, but these plans usually optimize a single link and lack systematic technical integration of the entire process of capture, temporary holding and transportation.

[0005] The Chinese invention patent application (publication number: CN111758634A, publication date: 2020-10-13) discloses a method for improving the survival rate of crayfish seedlings during transportation. The method involves culturing in a micro-flow state and stimulating the concentrated molting of crayfish seedlings in advance to promote the hardening of the shells and improve the quality and pressure resistance of crayfish seedlings. The method also involves selecting strong shrimp seedlings for moisturizing transportation to improve the survival rate of crayfish during transportation and promote the off-site breeding of crayfish. However, there is currently no public report on a method for systematically improving the survival rate of Macrobrachium rosenbergii seedlings during fishing and transportation. Summary of the invention

[0006] In order to solve the above-mentioned technical problems, the present invention provides an innovative method for catching and transporting Macrobrachium rosenbergii broodstock, which combines pathogen detection, fishing tool optimization, the application of shrimp shell hardening promoters before fishing, disinfection of transportation water before fishing, temporary storage after fishing, and the use of anti-stress plant extracts in water transportation, to systematically solve the key problems in the prior art, thereby significantly improving the survival rate and health level of the broodstock during fishing and transportation.

[0007] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: A method for catching and transporting Macrobrachium rosenbergii broodstock, the method comprising the following steps: (1) Pathogenic detection: Pathogenic detection is performed no less than twice a month before catching the broodstock, and the detection results are all negative.

[0008] (2) Preparation before fishing: Three weeks before fishing, spray shrimp shell hardening accelerator (concentration of 2 mg / L-5 mg / L) into the shrimp breeding pond 1-2 times a week. Stop feeding one day before fishing, and improve the shrimp's stress resistance by setting a specific water flow environment. The water flow meets the NY5051 standard.

[0009] (3) Fishing: The fishing method is to use a combination of drag net and dry pond fishing. The fishing tools include a catch net and a trap box. The mesh sizes are 2.0 cm-3.0 cm and 2.5 cm-3.3 cm respectively; (4) Temporary culture in cages: Cages are set up in the clean water area to temporarily culture the shrimps after fishing. 1 mg / L-2 mg / L of anti-stress plant extracts and shrimp shell hardening accelerators (concentration of 2 mg / L-5 mg / L) are added to the temporary culture water, and the oxygenation equipment is turned on to alleviate the stress response of the shrimps caused by the fishing operation and the respiratory hypoxia problem caused by the muddy water produced by the net. The temporary culture density is 10 kg / m³-30 kg / m³, and the temporary culture time is 0.5-2 hours.

[0010] (5) Transport with water: Add 1mg / L-2mg / L of anti-stress plant extract to the transport water, control the transport density at 50kg / m³-125kg / m³, use a box-type temperature-controlled vehicle or a customized shrimp transport vehicle, and record environmental parameters including air temperature, water temperature, dissolved oxygen content, pH value, etc. throughout the transportation process.

[0011] (6) Post-transportation processing: After the broodstock arrive at the destination, they are temporarily kept for 24-48 hours. The water temperature of the temporary holding pond is controlled at 20℃-21℃. Dead and defective individuals are disposed of harmlessly, and the remaining broodstock are placed in the broodstock cultivation pond.

[0012] Preferably, the etiological detection uses a real-time fluorescence PCR method to detect major pathogens of Macrobrachium rosenbergii including decapoda iridovirus, Nodavirus, dicistrovirus, infectious precocious virus, etc.

[0013] Preferably, the net pulling and scraping operations during the fishing process need to be carried out at a water depth of 50cm-100cm, and the operation should be repeated 3-4 times to achieve a fishing rate of more than 80%; after catching in the dry pond, the captured shrimps are immediately placed in the cages under the conditions of the oxygenation device, and the dissolved oxygen concentration in the water is continuously monitored.

[0014] Preferably, the shrimp shell hardening accelerator comprises the following components by mass percentage: 60%-70% chitosan derivative, 10%-20% calcium ion chelate, 5%-10% diatom extract, 3%-5% magnesium ion supplement, 3%-5% polysaccharide active agent and 1%-3% vitamin complex.

[0015] The functions of the ingredients in the shrimp shell hardening accelerator formula are as follows: Chitosan derivatives: Water-soluble chitosan (molecular weight 50,000-100,000 Da, degree of deacetylation ≥85%) is used as the main ingredient to enhance the hardness and antibacterial properties of shrimp shells.

[0016] Calcium ion chelate: The chelate formed by chitosan and calcium gluconate provides a directly absorbable calcium source to promote the calcification process of shrimp shells.

[0017] Diatom extract: rich in silicon (≥30%), which acts as a structural reinforcement factor in the hardening process of shrimp shells.

[0018] Magnesium ion supplement: added in the form of magnesium chloride or magnesium sulfate to regulate the calcium-magnesium balance in shrimp and promote the synergistic effect of muscle and shrimp shell hardening.

[0019] Polysaccharide active agent: seaweed polysaccharides (such as fucoidan) or xanthan gum are used to provide a synergistic hardening effect while improving the shrimp's anti-stress ability.

[0020] Vitamin complex: mainly vitamin C, vitamin E and vitamin D3, which enhance the shrimp's antioxidant capacity and calcium absorption efficiency.

[0021] Preferably, the anti-stress plant extract comprises 20-40% echinacea polysaccharide, 18-25% peppermint essential oil, 5-15% ginsenoside, 5-15% baicalin, and 2-8% citrus bioflavonoids. More preferably, the anti-stress plant extract further comprises 5-15% bitter orange extract, 2-8% rosmarinic acid, 2-8% vegetable tannins, and 2-8% aloe extract.

[0022] The effects of the components in the anti-stress plant extract are as follows: Echinacea polysaccharide ( Echinacea purpurea Polysaccharides can improve shrimp immunity, activate the nonspecific immune system, and reduce the risk of pathogen infection during transportation.

[0023] Peppermint essential oil ( Mentha piperita Essential Oil) calms the shrimp's nervous system, reduces stress responses caused by environmental changes, and has antioxidant and antibacterial effects.

[0024] Ginsenosides enhance shrimp metabolism and improve tolerance and recovery during transportation.

[0025] Baicalin: Anti-inflammatory and antioxidant, reduces inflammation and free radical damage caused by the dense environment during transportation.

[0026] Bitter Orange Extract ( Citrus aurantium Extract, rich in alkaloids) provides energy regulation support and promotes shrimp body activity under stress.

[0027] Citrus Bioflavonoids promote microcirculation and enhance antioxidant capacity.

[0028] Rosmarinic Acid further reduces oxidative stress and has antimicrobial effects.

[0029] Tannins protect cell membranes and reduce physical and chemical damage during transport.

[0030] Aloe vera extract ( Aloe Vera Extract) provides moisturizing and anti-inflammatory effects and stabilizes the osmotic pressure inside and outside cells.

[0031] As a preference, the dissolved oxygen content in the transport water is always kept above 5 mg / L, and the temperature difference is controlled within 1°C; the insulation ice used during transportation meets the SC / T9001 standard, and the transport water quality meets the NY5051 standard; And / or, reserve water for transportation 15 days before fishing, and use 20-30 grams of bleaching powder for every cubic meter of water for disinfection; 3 days before fishing, use the treated water for transportation to try to grow shrimp to ensure the safety of the transportation water.

[0032] Preferably, the density of the temporary holding pond is 10kg / m³-30kg / m³, and water circulation filtration equipment and oxygenation equipment are used to maintain water quality.

[0033] Preferably, electronic monitoring equipment is used throughout the entire process of fishing and transportation to record the operation process, including fishing time, transportation time and environmental parameters.

[0034] The present invention adopts the above technical solution and significantly improves the survival rate, health status and operation efficiency of seed shrimps through system optimization in key links such as fishing, temporary breeding and transportation. The specific technical effects are as follows: 1. Effectively reduce the risk of disease transmission: Through pathogen detection, especially the use of real-time fluorescence PCR technology, quantitative detection of major shrimp pathogens including decapod iridovirus, Nodavirus, dicistrovirus, and infectious precocious virus is carried out to ensure that all the caught shrimp are healthy individuals, reducing the risk of disease transmission from the source.

[0035] 2. Improve the hardening degree of the shrimp shells of breeding shrimps: Add shrimp shell hardening accelerators (concentration of 2 mg / L-5 mg / L) to the breeding water before fishing. Through multifunctional ingredients such as chitosan derivatives, calcium ion chelates and diatom extracts, the hardness of the shrimp shells can be enhanced, and their ability to resist physical damage during transportation can be improved. At the same time, immunity can be enhanced through polysaccharide active agents and vitamin complexes.

[0036] 3. Reduce the mortality rate during transportation: Add anti-stress plant extracts (concentration of 1 mg / L-3 mg / L) to the transportation water, including multifunctional components such as echinacea polysaccharides, peppermint essential oil, ginsenosides and baicalin, to significantly reduce the stress response of the shrimp caused by environmental changes (such as temperature differences and water quality fluctuations), and ensure the vitality of the shrimp during transportation.

[0037] 4. Realize efficient monitoring and management of the entire process: IoT devices can be used to monitor key parameters in the fishing and transportation process (such as dissolved oxygen, temperature, pH value, and transportation time) in real time, and combined with electronic recording systems to achieve traceability management of the entire process, effectively reducing operational risks and ensuring process transparency and standardization.

[0038] In summary, the present invention significantly reduces the loss rate of seed shrimp (survival rate increased by more than 10%), improves aquaculture production efficiency, and reduces economic losses by controlling pathogenic risks at the source, promoting hardening of shrimp shells, optimizing fishing tools, temporary cage culture, reducing stress, and controlling the transportation environment. At the same time, it adopts green preparations (such as chitosan derivatives and plant extracts) to achieve eco-friendliness. It provides an efficient, safe, and sustainable technical solution for the cultivation and circulation of Macrobrachium rosenbergii seed shrimp, and has significant promotion and application value. DETAILED DESCRIPTION

[0039] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Example 1 1. Pathogenic detection One month before fishing, pathogen detection is carried out every two weeks, and real-time fluorescence quantitative PCR technology is used to sample and test the seed shrimp, focusing on the detection of common shrimp pathogens such as decapod iridovirus, Nodavirus, dicistrovirus, infectious precocious virus, etc. When the test results are all negative, it is confirmed that the fishing batch is healthy and can be used.

[0041] 2. Preparation before fishing Three weeks before fishing, spray shrimp shell hardening accelerator (concentration of 2 mg / L-5 mg / L) into the shrimp breeding pond 1-2 times a week. The specific formula of shrimp shell hardening accelerator includes chitosan derivative (65%), calcium ion chelate (15%), diatom extract (8%), magnesium ion supplement (4%), polysaccharide active agent (5%) and vitamin complex (3%). The chitosan derivative uses water-soluble chitosan with a molecular weight of 50,000-100,000Da and a deacetylation degree of ≥85%; the calcium ion chelate is a chelate formed by chitosan and calcium gluconate; the magnesium ion supplement is magnesium chloride; the polysaccharide active agent is seaweed polysaccharide; the vitamin complex is vitamin C (2%), vitamin E (0.8%) and vitamin D3 (0.2%).

[0042] Stop feeding one day before fishing, and improve the shrimp's ability to resist stress by setting up a specific water flow environment. The water flow meets the NY5051 standard. Reserve transportation water 15 days before fishing, and use 20-30 grams of bleaching powder per cubic meter of water for disinfection. Three days before fishing, use the treated transportation water to try to raise shrimps to ensure the safety of the transportation water. Stop feeding one day before fishing to empty the digestive tract of the shrimps and avoid metabolic waste from contaminating the water during transportation. At the same time, create a water flow environment with a flow rate controlled at 0.1 to 0.3 meters per second, which meets the water quality standard of NY5051 and improves the stress resistance of shrimp fishing.

[0043] 3. Fishing The fishing operation is carried out when the water depth is 50 to 100 cm. The specific method is as follows: 1) Pull net and scrape catch: Use a catch net with a mesh size of 2.0 cm to 3.0 cm, slowly pull it from one end of the pond to the other, with the bottom of the net close to the bottom of the pond, and perform the operation 2 to 4 times to ensure that the catch rate exceeds 80%.

[0044] 2) Dry pond capture: Lower the pond water level to below 30 cm, use a trap (mesh size 2.5 cm to 3.3 cm) or a dip net to collect the remaining shrimp. After the capture, immediately transfer the shrimp to a temporary cage under aeration conditions.

[0045] 4. Temporary culture in cages The caught shrimps are concentrated in cages for temporary rearing. The process is as follows: Addition of shrimp shell hardening accelerator: Add 3 mg / L of shrimp shell hardening accelerator and 1.5 mg / L of anti-stress plant extract to the temporary holding water.

[0046] The specific formula of the shrimp shell hardening accelerator includes chitosan derivatives (65%), calcium ion chelates (15%), diatom extracts (8%), magnesium ion supplements (4%), polysaccharide active agents (5%) and vitamin complexes (3%). The chitosan derivatives use water-soluble chitosan with a molecular weight of 50,000 to 100,000 Da and a deacetylation degree of ≥85%; the calcium ion chelate is a chelate formed by chitosan and calcium gluconate; the magnesium ion supplement is magnesium chloride; the polysaccharide active agent is seaweed polysaccharide; and the vitamin complex is vitamin C (2%), vitamin E (0.8%) and vitamin D3 (0.2%).

[0047] The specific formula of stress-resistant plant extracts includes Echinacea polysaccharides (30%), peppermint essential oil (20%), ginsenosides (10%), baicalin (10%), citrus bioflavonoids (5%), bitter orange extract (10%), rosmarinic acid (5%), aloe extract (5%) and plant tannins (5%).

[0048] Water quality management: The dissolved oxygen concentration is maintained at not less than 5 mg / L through aeration equipment, the water temperature is controlled at 20℃~23℃, the temporary stocking density is 25kg / m³, and the temporary stocking time is 1 hour.

[0049] 5. Transport with water Transfer the broodstock to the live shrimp transport tank. The transport operation is as follows: Preparation of transport water: Use pre-sterilized transport water, which should account for 3 / 4 of the volume of the transport water tank.

[0050] Addition of anti-stress plant extracts: Add anti-stress plant extracts at a concentration of 1.5 mg / L to the transport water. The specific formula includes echinacea polysaccharides (30%), peppermint essential oil (20%), ginsenosides (10%), baicalin (10%), citrus bioflavonoids (5%), bitter orange extract (10%), rosmarinic acid (5%), aloe extract (5%) and plant tannins (5%).

[0051] Transportation process: The transportation density is controlled at 60 kg / m³~80 kg / m³, live shrimp transport vehicles are used, and water environmental parameters are monitored in real time (dissolved oxygen ≥5 mg / L, temperature difference ≤1°C). Information such as transportation temperature, dissolved oxygen, and air temperature are recorded through electronic recording equipment.

[0052] 6. Post-transportation processing After the seed shrimp arrive at the destination, follow the steps below: Temporary recovery: transfer the breeding shrimp to the temporary holding pond, control the water temperature at 20℃~21℃, the temporary holding density at 5 kg / m³~10 kg / m³, and the temporary holding time shall not exceed 48 hours.

[0053] Sorting and processing: dead and defective shrimps are selected for harmless disposal, and the remaining healthy shrimps are transferred to the intensive cultivation stage.

[0054] Example 2 Before fishing and during temporary cage rearing, the method disclosed in the Chinese invention patent application (publication number: CN111758634A, publication date: 2020-10-13) is used to add ionic calcium and hard shell treasure to the feed. Other technical features are the same as those in Example 1.

[0055] Example 3 When transported with water, the anti-stress plant extracts added are 35% echinacea polysaccharide, 20% peppermint essential oil, 10% ginsenoside, 10% baicalin, and 5% citrus bioflavonoids. Other technical features are the same as in Example 1.

[0056] Comparative Example 1 No cage culture is performed, and other technical features are the same as those in Example 1.

[0057] Comparative Example 2 No cage culture is performed, and no anti-stress plant extract is added during transportation with water. Other technical features are the same as those in Example 1.

[0058] Comparative Example 3 When transported with water, no anti-stress plant extract is added, and other technical features are the same as in Example 1.

[0059] The following comparative test of Example 1 and the comparative example verifies the effects of temporary culture in separate ponds and the addition of shrimp shell hardening accelerators and anti-stress plant extracts on the survival rate, vitality and health status of Macrobrachium rosenbergii seedlings, thereby proving the technical effect of the present invention.

[0060] 1. Experimental design Test subjects: Macrobrachium rosenbergii fry caught from the same pond, with 120 samples in each test group.

[0061] Test environment: transportation distance 300 kilometers, transportation time 4 hours, transportation temperature controlled at 20℃~21℃.

[0062] 2. Evaluation indicators: Transport survival rate (%); Post-transport vitality score: the score range is 1 to 5 (1 for low vitality and 5 for high vitality); Defective rate after transportation (%): includes the proportion of damaged individuals and individuals with disease symptoms.

[0063] 3. Test results

[0064] 4. Results Analysis 1) The importance of temporary rearing in separate ponds Embodiment 1, embodiment 2 and embodiment 3 all adopt cage temporary culture technology, and their survival rates (98.5%, 95.4%, 97.2%) are significantly higher than those of comparative example 1 (89.7%) and comparative example 2 (84.6%) which are not subjected to cage temporary culture.

[0065] Temporary cage culture effectively alleviates the stress response of broodstock shrimp after fishing. At the same time, through water quality management and the addition of shrimp shell hardening promoters, the transportation adaptability and damage resistance of broodstock shrimp are enhanced.

[0066] Comparative Example 2, in which no cage culture was performed and no anti-stress plant extracts were added, had a defective rate as high as 12.4%, further demonstrating the importance of cage culture.

[0067] 2) The role of shrimp shell hardening accelerator The defective rates (1.2%, 3.0%, 2.4%) of Examples 1, 2, and 3, in which the shrimp shell hardening accelerator was added during cage temporary culture, were significantly lower than those of Comparative Example 2 (12.4%) in which the shrimp shell hardening accelerator was not added. This indicates that the shrimp shell hardening accelerator significantly reduces physical damage during transportation by increasing the hardness and damage resistance of the shrimp shell.

[0068] The defective rate of Example 1 is lower than that of Example 2 (1.2% vs 3.0%), indicating that the shrimp shell hardening accelerator formula (such as chitosan derivatives and calcium ion chelates) of the present invention is better than ionic calcium and hard shell treasure in the prior art.

[0069] 3) Effects of anti-stress plant extracts Both Example 1 and Example 3 added anti-stress plant extracts, and their survival rates (98.5%, 97.2%) and vitality scores (4.8 points, 4.5 points) were significantly higher than those of Comparative Example 1 (survival rate 89.7%, vitality score 3.5 points) and Comparative Example 3 (survival rate 91.2%, vitality score 3.8 points) without adding anti-stress plant extracts.

[0070] The anti-stress plant extract formula (including Echinacea polysaccharides, peppermint essential oil, etc.) used in Example 1 was better than that in Example 3 (single formula), with the survival rate and vitality score being 1.3% and 0.3 points higher, respectively, indicating that the synergistic effect of multiple components has significant advantages in alleviating transportation stress.

[0071] 4) Comprehensive technical effects In Example 1, the combination of cage temporary culture, shrimp shell hardening accelerator and anti-stress plant extracts has a survival rate (98.5%) that is 13.9% higher than that of Comparative Example 2 (84.6%), a vitality score (4.8 points) that is 1.7 points higher than that of Comparative Example 2 (3.1 points), and a defective rate (1.2%) that is significantly lower than that of Comparative Example 2 (12.4%). This shows that the combined technical solution of Example 1 has the best technical effect.

[0072] The above is a description of the embodiments of the present invention. Through the above description of the disclosed embodiments, professionals and technicians in the field can implement or use the present invention. Various modifications to these embodiments will be apparent to professionals and technicians in the field. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown in this article, but will conform to the widest range consistent with the principles and novelties disclosed herein.

Claims

1. A method for catching and transporting Macrobrachium rosenbergii broodstock, characterized in that: The method comprises the following steps: (1) Pathogen testing: Pathogen testing should be conducted no less than twice a month before catching the broodstock shrimp, and the test results should all be negative; (2) Preparation before fishing: Three weeks before fishing, spray shrimp shell hardening accelerator into the shrimp breeding pond 1-2 times a week; stop feeding one day before fishing; (3) Fishing: The fishing method is to use a combination of drag net and dry pond fishing. The fishing tools include a catch net and a trap box. The mesh sizes are 2.0 cm-3.0 cm and 2.5 cm-3.3 cm respectively; (4) Temporary culture in net cages: net cages are set up in the clean water area to temporarily culture the shrimps after fishing. 1 mg / L-2 mg / L of anti-stress plant extracts and 2 mg / L-5 mg / L of shrimp shell hardening accelerators are added to the temporary culture water, and the oxygenation equipment is turned on to alleviate the stress response of the shrimps caused by the fishing operation and the respiratory hypoxia problem caused by the muddy water produced by the net. The temporary culture density is 10 kg / m³-30 kg / m³, and the temporary culture time is 0.5-2 hours; (5) Transport with water: Add 1mg / L-2mg / L of anti-stress plant extract to the transport water, control the transport density at 50kg / m³-125kg / m³, use a box-type temperature-controlled vehicle or a customized shrimp transport vehicle, and record environmental parameters including air temperature, water temperature, dissolved oxygen content, and pH value throughout the transportation process; (6) Post-transportation processing: After the broodstock arrive at the destination, they are temporarily kept for 24-48 hours. The water temperature of the temporary holding pond is controlled at 20℃-21℃. Dead and defective individuals are disposed of harmlessly, and the remaining broodstock are placed in the broodstock cultivation pond.

2. The method according to claim 1, characterized in that: Pathogenic detection used real-time fluorescence PCR method to detect the main pathogens of Macrobrachium rosenbergii, including decapoda iridovirus, Nodavirus, dicistrovirus, and infectious precocious virus.

3. The method according to claim 1, characterized in that The net-pulling and scraping operations during the fishing process must be carried out at a water depth of 50cm-100cm, and the operation must be repeated 3-4 times to achieve a capture rate of more than 80%; after catching in the dry pond, the captured shrimps are immediately placed in the net cage under the conditions of the oxygenation device, and the dissolved oxygen concentration in the water is continuously monitored.

4. The method according to claim 1, characterized in that: The shrimp shell hardening accelerator comprises the following components by mass percentage: 60%-70% chitosan derivative, 10%-20% calcium ion chelate, 5%-10% diatom extract, 3%-5% magnesium ion supplement, 3%-5% polysaccharide active agent and 1%-3% vitamin complex.

5. The method according to claim 3, characterized in that: The chitosan derivative adopts water-soluble chitosan with a molecular weight of 50,000-100,000Da and a deacetylation degree ≥85%; the calcium ion chelate is a chelate formed by chitosan and calcium gluconate; the magnesium ion supplement is magnesium chloride or magnesium sulfate; the polysaccharide active agent is seaweed polysaccharide or xanthan gum; and the vitamin complex is vitamin C, vitamin E and vitamin D3.

6. The method according to claim 1, characterized in that The anti-stress plant extract comprises 20-40% of echinacea polysaccharide, 18-25% of peppermint essential oil, 5-15% of ginsenoside, 5-15% of baicalin, and 2-8% of citrus bioflavonoids.

7. The method according to claim 6, characterized in that The anti-stress plant extract further comprises 5-15% bitter orange extract, 2-8% rosmarinic acid, 2-8% vegetable tannins and 2-8% aloe extract.

8. The method according to claim 1, characterized in that The dissolved oxygen content in the transport water is always kept above 5mg / L, and the temperature difference is controlled within 1°C; the insulation ice used during transportation complies with the SC / T9001 standard, and the transport water quality complies with the NY5051 standard; And / or, reserve transport water 15 days before fishing and disinfect it with 20-30 grams of bleach per cubic meter of water; Three days before fishing, use treated transport water to try to grow shrimp to ensure the safety of the transport water.

9. The method according to claim 1, characterized in that: The density of the temporary holding pond is 10kg / m³-30kg / m³, and water circulation filtration equipment and oxygenation equipment are used to maintain water quality.

10. The method according to claim 1, characterized in that Electronic monitoring equipment is used throughout the fishing and transportation process to record the operation process, including fishing time, transportation time and environmental parameters.

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