Kit and hematology analyzer

Abstract

One end of one of a front pool electrode and a rear pool electrode of the kit passes through a microporous sheet and extends to one side where the other electrode is located, and the other end of the front pool electrode and the rear pool electrode and the other end of the other electrode are exposed out of the same side face of a shell. The kit can be in butt joint with the forebay electrode and the afterbay electrode in one direction when being electrically connected with the hematology analyzer, the alignment process of the kit and the hematology analyzer is simple, the alignment accuracy of the hematology analyzer and the kit is improved, the electrical connection stability of the kit and the hematology analyzer is improved, and the detection accuracy of the hematology analyzer is improved. The possibility of faults such as failure in detection caused by inaccurate butt joint of the kit and the hematology analyzer is reduced, and the use experience of a user is improved.

Description

Technical Field

[0001] The present application relates to the technical field of in vitro diagnosis, and in particular to a kit and a blood cell analyzer. Background Art

[0002] Hematology analyzer is one of the most widely used instruments in clinical testing in hospitals. In order to ensure that all traces of the previous blood sample are cleaned before the next blood sample test, traditional hematology analyzers usually need to occupy a large amount of space to set up a cleaning system. The cleaning system is not only complex in structure and has many components, but also requires a large amount of reagents during the cleaning process, which takes a long time, making traditional hematology analyzers unsuitable for instant testing scenarios.

[0003] Compared with traditional blood cell analyzers, the POCT blood cell analyzer has made great simplifications in instrument components, reducing the complexity and production cost of the product, allowing the POCT blood cell analyzer to perform rapid testing immediately at the sampling site with simple and quick operation.

[0004] However, when an existing POCT blood cell analyzer receives a test kit, front cell electrodes and rear cell electrodes are respectively provided on both sides of the test kit. The blood cell analyzer needs to be electrically connected to the front cell electrodes and rear cell electrodes on both sides of the test kit. The test kit and the blood cell analyzer need to be positioned at two connection positions, resulting in an unstable electrical connection between the test kit and the blood cell analyzer. Summary of the invention

[0005] In order to solve the above problems existing in the prior art, the present application provides a kit and a blood cell analyzer.

[0006] In order to solve the technical problems existing in the prior art, the present application provides a test kit, including a shell, a detection cell, a microporous sheet, a front cell electrode and a rear cell electrode; the detection cell is arranged in the shell, including a front cell and a rear cell, and the rear cell is arranged on one side of the front cell; the microporous sheet is located between the front cell and the rear cell, the microporous sheet is provided with micropores, and the front cell and the rear cell are connected through the micropores; the front cell electrode and the rear cell electrode, one end of the rear cell electrode is located in the rear cell, one end of the front cell electrode is located in the front cell, the other end of one of the front cell electrode and the rear cell electrode extends over the microporous sheet to the side where the other electrode is located, and is exposed on the same side of the shell as the other end of the other electrode.

[0007] Optionally, one end of the front cell electrode passes over the microporous sheet and is exposed on the same side of the shell together with the other end of the rear cell electrode.

[0008] Optionally, the front cell electrode and the rear cell electrode have the same length exposed from the shell.

[0009] Optionally, the rear cell electrode and the front cell electrode are arranged along the gravity direction of the reagent box.

[0010] Optionally, the test kit includes a connection port and a pressure chamber, the pressure chamber is connected to the rear cell, the connection port is connected to the pressure chamber, and the connection port, the front cell electrode and one end of the rear cell electrode are all exposed on the same side of the shell.

[0011] Optionally, the pressure chamber and the back pool are arranged along the gravity direction of the reagent box.

[0012] Optionally, a first opening is provided at the bottom of the forecell, one end of the forecell electrode extends to a position of the forecell corresponding to the first opening, and the other end of the forecell electrode passes over the microporous sheet.

[0013] Optionally, the rear cell includes a first cell body, a second cell body and a cover member, the first cell body is connected to the second cell body, the cover member is installed at one end of the shell close to the rear cell electrode, the first cell body, the second cell body and the cover member are enclosed to form the rear cell, the cover member includes a cover body and abutment, one end of the abutment is connected to the cover body, and the other end of the abutment abuts the microporous sheet.

[0014] In order to solve the technical problems existing in the prior art, the present application provides a blood cell analyzer, including a detection seat. The detection seat is used to install the above test kit; the detection seat includes a power supply component, the power supply component is used to abut the front cell electrode and the rear cell electrode, and the power supply component is used to provide a detection current for the test kit, so that the blood cell analyzer detects the sample to be tested in the test kit.

[0015] In order to solve the technical problems existing in the prior art, the present application provides a blood cell analyzer, including a detection seat and a power supply assembly. The detection seat is used to receive the above test kit, and the detection seat is also used to move the test kit to a detection position; the power supply assembly is used to dock with the front cell electrode and the rear cell electrode at the detection position, and the power supply assembly is used to provide a detection current for the test kit, so that the blood cell analyzer detects the sample to be tested in the test kit.

[0016] Optionally, the shell includes a first side surface and a second side surface, the first side surface and the second side surface are arranged along the installation direction or movement direction of the reagent kit, the second side surface is located in front of the first side surface, and the front cell electrode and the rear cell electrode are both exposed on the second side surface of the shell.

[0017] Compared with the prior art, one end of one of the front cell electrode and the rear cell electrode of the test kit provided by the present application extends beyond the microporous sheet to the side where the other electrode is located, while the other end of the other electrode is exposed on the same side of the shell, so that when the test kit is electrically connected to the blood cell analyzer, it can be docked with the front cell electrode and the rear cell electrode in one direction, the alignment process of the test kit and the blood cell analyzer is simple, the alignment accuracy of the blood cell analyzer and the test kit is improved, and then the electrical connection stability of the test kit and the blood cell analyzer is improved, the possibility of failures such as failure to detect due to inaccurate docking between the test kit and the blood cell analyzer is reduced, and the user experience is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 It is a schematic structural diagram of an embodiment of the kit provided by the present application;

[0020] Figure 2 yes Figure 1 A cross-sectional view of the reagent kit at section AA;

[0021] Figure 3 is a schematic structural diagram of another embodiment of the kit provided by the present application;

[0022] Figure 4 It is a structural schematic diagram of another embodiment of the kit provided by the present application;

[0023] Figure 5 yes Figure 3 A schematic diagram of the structure of the middle cover assembly;

[0024] Figure 6 It is a structural schematic diagram of an embodiment of a blood cell analyzer provided by the present application;

[0025] Figure 7 It is a structural schematic diagram of another embodiment of the blood cell analyzer provided by the present application. DETAILED DESCRIPTION

[0026] The present application is further described in detail below in conjunction with the accompanying drawings and examples. It is particularly noted that the following examples are only used to illustrate the present application, but are not intended to limit the scope of the present application. Similarly, the following examples are only some embodiments of the present application rather than all embodiments, and all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present application.

[0027] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0028] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "set", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or connected through an intermediate medium. For ordinary technicians in this field, if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of this application, the directional indications are only used to explain the relative position relationship, movement, etc. between the components in a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0029] The present application first proposes a test kit, which is applied to a blood cell analyzer, and the blood cell analyzer is specifically a POCT blood cell analyzer, which is used in a point-of-care testing scenario; the test kit is used to store samples to be tested, and the blood cell analyzer is used to receive the test kit and test the samples to be tested in the test kit. The blood cell analyzer can be used to analyze major examination items such as red blood cells, white blood cells and platelets in a routine blood test to obtain test data of relevant indicators of major examination items such as red blood cells, white blood cells and platelets.

[0030] For example, the test data may include test data of the leukocyte system, such as white blood cell count (WBC), granulocyte number, granulocyte percentage, mid number, mid percentage, lymphocyte number, lymphocyte percentage, The test data may also include test data of the red blood cell system, such as red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width coefficient of variation (RDW-CV), red blood cell distribution width standard deviation (RDW-SD), etc. The test data may also include test data of the platelet system, such as platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW), number of large platelets (P-LCR), large platelet ratio (P-LCC), platelet packed volume (PCT) and other indicators.

[0031] See also Figure 1-2 , Figure 1 is a schematic structural diagram of an embodiment of the kit provided in the present application, Figure 2 yes Figure 1 The cross-sectional view of the kit at section AA. Figure 1-2 As shown, the reagent kit 10 of this embodiment includes a housing 110 , a detection cell 210 , a microporous sheet 310 , a front cell electrode 212 and a rear cell electrode 214 .

[0032] Specifically, the detection pool 210 is arranged in the shell 110, including a front pool 211 and a rear pool 213, and the rear pool 213 is arranged on one side of the front pool 211; the microporous sheet 310 is located between the front pool 211 and the rear pool 213, and the microporous sheet 310 is provided with micropores, and the front pool 211 and the rear pool 213 are connected through the micropores; wherein, one end of the front pool electrode 212 is located in the front pool 211, and one end of the rear pool electrode 214 is located in the rear pool 213, and the other end of one of the front pool electrode 212 and the rear pool electrode 214 extends over the microporous sheet 310 to the side where the other electrode of the front pool electrode 212 and the rear pool electrode 214 is located, and the other end of the other electrode is exposed on the same side of the shell 110, so that the other end of the front pool electrode 212 and the rear pool electrode 214 can be easily electrically connected to the blood cell analyzer.

[0033] The microporous sheet 310 is located between the front pool 211 and the rear pool 213, specifically on the adjacent side walls of the front pool 211 and the rear pool 213. Micropores are provided on the microporous sheet 310, and the area near the micropores is the detection area of ​​the blood cell analyzer. The reagent kit 10 is connected to the blood cell analyzer, which is used to detect the test sample flowing through the microporous sheet 310. The power supply component of the blood cell analyzer is connected to the front pool electrode 212 and the rear pool electrode 214 of the detection pool 210, and the power supply component is used to power the constant current circuit of the detection pool 210, so that the detection pool 210 is in a constant current environment.

[0034] One end of the front cell electrode 212 is located in the front cell 211, and one end of the rear cell electrode 214 is located in the rear cell 213, so that the front cell electrode 212 contacts the first test sample in the front cell 211 and conducts electricity, one end of one of the front cell electrode 212 and the rear cell electrode 214 passes over the microporous sheet 310 and extends to the side where the other electrode of the front cell electrode 212 and the rear cell electrode 214 is located, and the other ends of the front cell electrode 212 and the rear cell electrode 214 are both exposed on the same side of the housing 110. Specifically, one end of one electrode is located on one side of the microporous sheet 310, and the other electrode is located on the other side of the microporous sheet 310, that is, one end of the front cell electrode 212 and the rear cell electrode 214 are respectively located on both sides of the microporous sheet 310, and the other ends of the front cell electrode 212 and the rear cell electrode 214 are located on the other side of the microporous sheet 310.

[0035] Exemplarily, in one embodiment, when the front cell 211 is positioned close to the side where the front cell electrode 212 and the rear cell electrode 214 are exposed on the shell 110, the electrodes are exposed on the side of the shell 110, the front cell 211 and the rear cell 213 are arranged in sequence along the length direction of the reagent box 10. At this time, one end of the rear cell electrode 214 is located on one side of the microporous sheet 310, and one end of the front cell electrode 212, the other end of the front cell electrode 212 and the other end of the rear cell electrode 214 are all located on the other side of the microporous sheet 310, that is, the front cell electrode 212 is as a whole located on one side of the microporous sheet 310 close to the side where the electrode is exposed on the shell 110, and one end of the rear cell electrode 214 is arranged in the rear cell 213 over the microporous sheet 310. In another embodiment, when the rear cell 213 is positioned close to the side of the front cell electrode 212 and the rear cell electrode 214 exposed on the shell 110, the electrodes are exposed on the side of the shell 110, and the rear cell 213 and the front cell 211 are arranged along the length direction of the reagent box 10. At this time, one end of the front cell electrode 212 is located on one side of the microporous sheet 310, and one end of the rear cell electrode 214, the other end of the rear cell electrode 214 and the other end of the front cell electrode 212 are all located on the other side of the microporous sheet 310, that is, the rear cell electrode 214 is as a whole located on one side of the microporous sheet 310 close to the side of the electrode exposed on the shell 110, and one end of the front cell electrode 212 is arranged in the front cell 211 over the microporous sheet 310.

[0036] Since blood cells are poor conductors relative to diluents, when the first test sample in the front pool 211 flows through the micropores to the rear pool 213 under power drive, the resistance between the front pool electrode 212 and the rear pool electrode 214 changes when the blood cells of the first test sample flow through the micropores, thereby generating a voltage change at both ends of the front pool electrode 212 and the rear pool electrode 214 and forming a pulse signal. The blood cell analyzer records the pulse signal and determines the type, volume and number of blood cells according to the set threshold value to obtain test data.

[0037] And, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 are exposed on the same side of the housing 110. The housing 110 may include a first side, a second side, a third side and a fourth side that are enclosed, the first side is located on the side of the housing 110 close to the front pool 211 and the rear pool 213, the second side is arranged opposite to the first side, the third side is adjacent to the first side and the second side, and the fourth side is arranged opposite to the third side. In an optional embodiment, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 can be exposed at any position of the first side, the second side, the third side and the fourth side. In an optional embodiment, the front pool electrode 212 and the rear pool electrode 214 can be arranged to be a columnar structure extending along the length direction of the reagent box 10, and the front pool electrode 212 and the rear pool electrode 214 can also be an electrode spring sheet, which is bent and arranged in a preset direction inside the reagent box 10, so that the user can adjust the exposed position of the front pool electrode 212 and the rear pool electrode 214 according to actual needs, which is not specifically limited here.

[0038] In the embodiment of the present application, since one end of one of the front cell electrode 212 and the rear cell electrode 214 of the reagent kit 10 extends beyond the microporous sheet 310 to the side where the other electrode is located, and the other end of the other electrode is exposed on the same side of the shell 110, the reagent kit 10 can be docked with the front cell electrode 212 and the rear cell electrode 214 in one direction when electrically connected to the blood cell analyzer, the alignment process of the reagent kit 10 and the blood cell analyzer is simple, the alignment accuracy of the blood cell analyzer and the reagent kit 10 is improved, and the electrical connection stability of the reagent kit 10 and the blood cell analyzer is improved, the possibility of failures such as inability to detect due to inaccurate docking between the reagent kit 10 and the blood cell analyzer is reduced, and the user experience is improved.

[0039] Furthermore, the test kit 10 may also include at least one non-detection pool, which can be used to store diluents, hemolytic agents, cleaning solutions and other reagents required for detection, and can also be used to provide pipette heads and other component support for the pipetting assembly of the blood cell analyzer. The detection pool 210 and the non-detection pool of the test kit 10 are arranged side by side along the length direction of the test kit 10 (i.e., the arrangement direction of the front pool 211 and the rear pool 213) on the side of the front pool 211 away from the rear pool 213, that is, the detection pool 210 and at least one non-detection pool of the test kit 10 are arranged linearly to reduce the volume of the test kit 10 in the width direction. At this time, the front pool electrode 212 and the rear pool electrode 214 exposed on the side of the shell 110 can be the side of the shell 110 arranged opposite to each other in the length direction, so that the other ends of the front pool electrode 212 and the rear pool electrode 214 can be electrically connected to the blood cell analyzer. Furthermore, since the various pools of the test kit 10 are arranged linearly, the pipetting component of the blood cell analyzer can perform pipetting operations between the various pools through one-dimensional movement. The blood cell analyzer does not need to set moving tracks in multiple directions, which further reduces the size of the blood cell analyzer and is conducive to the miniaturization of the blood cell analyzer.

[0040] In one embodiment, one end of the front cell electrode 212 passes over the microporous sheet 310 , and the other end of the front cell electrode 212 and the rear cell electrode 214 are exposed on the same side of the housing 110 .

[0041] Specifically, the rear cell electrode 214 is located on the side of the microporous sheet 310 away from the front cell 211, one end of the front cell electrode 212 passes over the microporous sheet 310 and is located in the front cell 211, and the other end of the front cell electrode 212 and the rear cell electrode 214 are exposed on the same side of the housing 110. That is, the rear cell electrode 214 is located as a whole on the side of the microporous sheet 310 close to the side where the electrode is exposed on the housing 110. In an optional embodiment, the front pool 211 and the rear pool 213 are arranged along the length direction of the reagent box 10, and the shell 110 includes a first side surface, a second side surface, a third side surface and a fourth side surface that are enclosed. The first side surface is located on the side of the rear pool 213 away from the front pool 211, the second side surface is arranged opposite to the first side surface, the third side surface is adjacent to the first side surface and the second side surface respectively, and the fourth side surface is arranged opposite to the third side surface. At this time, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 can be exposed at any position of the first side surface, the second side surface, and the third side surface; or, the other end of the front pool electrode 212 and the other end of the rear pool electrode 214 are both exposed on the first side surface to reduce the complexity of the internal structure.

[0042] In the embodiment of the present application, one end of the front cell electrode 212 passes over the microporous sheet 310, and the other ends of the front cell electrode 212 and the rear cell electrode 214 are exposed together on the same side of the shell 110, so that when the reagent kit 10 is electrically connected to the blood cell analyzer, it can be docked with the front cell electrode 212 and the rear cell electrode 214 in one direction. The alignment process of the reagent kit 10 and the blood cell analyzer is simple, which improves the alignment accuracy of the blood cell analyzer and the reagent kit 10, thereby improving the electrical connection stability between the reagent kit 10 and the blood cell analyzer, reducing the failure of detection due to inaccurate docking between the reagent kit 10 and the blood cell analyzer, and improving the user experience.

[0043] In one embodiment, the lengths of the front cell electrode 212 and the rear cell electrode 214 protruding from the housing 110 are equal.

[0044] Specifically, the lengths of the front cell electrode 212 and the rear cell electrode 214 protruding from the shell 110 are equal, so that the blood cell analyzer can dock with the front cell electrode 212 and the rear cell electrode 214 on the same contact surface through the power supply assembly, thereby reducing the possibility of faults such as poor contact in some positions when the lengths of the front cell electrode 212 and the rear cell electrode 214 protruding from the shell 110 are unequal, thereby improving the electrical connection stability between the test kit 10 and the blood cell analyzer.

[0045] In one embodiment, the rear cell electrode 214 and the front cell electrode 212 are arranged along the gravity direction of the reagent cartridge 10 .

[0046] Specifically, in this embodiment, the rear cell electrode 214 and the front cell electrode 212 are arranged along the gravity direction of the test kit 10, that is, the rear cell electrode 214 is located above the front cell electrode 212, and correspondingly, the position where the power supply component is docked with the rear cell electrode 214 and the front cell electrode 212 is arranged vertically, so that the blood cell analyzer can be electrically connected to the test kit 10 when stably receiving the test kit 10, thereby improving the electrical connection stability between the test kit 10 and the blood cell analyzer.

[0047] In one embodiment, a first opening is provided at the bottom of the forecell 211 , one end of the forecell electrode 212 extends to a position of the forecell 211 corresponding to the first opening, and the other end of the forecell electrode 212 passes over the microporous sheet 310 .

[0048] Specifically, a first opening is provided at the bottom of the fore cell 211, and one end of the fore cell electrode 212 extends to the position of the fore cell 211 corresponding to the first opening, so that one end of the fore cell electrode 212 contacts and conducts electricity with the first detection sample in the fore cell 211, and the bottom of the fore cell 211 is sealed by one end of the fore cell electrode 212 to maintain the relative stability of the first detection sample in the fore cell 211. One end of the rear cell electrode 214 is located in the rear cell 213, and the other end of the fore cell electrode 212 passes over the microporous sheet 310 and is exposed to the housing 110.

[0049] Furthermore, the blood cell analyzer is provided with a first detection component for impedance detection and a second detection component for optical detection, the first detection component is used to perform electrical impedance detection on the detection sample flowing through the microporous sheet 310, and the second detection component is used to perform colorimetric detection on the detection sample in the forecell 211. Since one end of the forecell electrode 212 extends to the position of the forecell 211 corresponding to the first opening, the position where the forecell electrode 212 contacts the first detection sample is located at the bottom of the forecell 211, and the detection position when the second detection component detects the first detection sample in the forecell 211 is located in the middle and upper part of the forecell 211, so that the detection areas of the first detection component and the second detection component are staggered to reduce the influence of impedance detection on optical detection and improve the detection accuracy of the blood cell analyzer.

[0050] In one embodiment, see Figure 3-5 , Figure 3 is a schematic structural diagram of another embodiment of the kit provided by the present application, Figure 4 is a schematic structural diagram of another embodiment of the kit provided in the present application, Figure 5 yes Figure 3 The structural diagram of the middle cover assembly. Figure 3-5 As shown, the rear cell 213 includes a first cell body 2131, a second cell body 2132 and a cover member 215. The first cell body 2131 is connected to the second cell body 2132. The cover member 215 is installed at one end of the shell 110 close to the rear cell electrode 214. The first cell body 2131, the second cell body 2132 and the cover member 215 are enclosed to form the rear cell 213. The cover member 215 includes a cover body 216 and abutment 217. One end of the abutment 217 is connected to the cover body 216, and the other end of the abutment 217 abuts against the microporous sheet 310.

[0051] Specifically, the first cell body 2131 and the second cell body 2132 are interconnected, and the first cell body 2131 and the second cell body 2132 are arranged along the gravity direction. The second cell body 2132 is used to accommodate the rear cell electrode 214. When the first detection sample in the front cell 211 flows to the rear cell 213 through the micropores on the microporous sheet 310, the waste liquid of the first detection sample moved from the front cell 211 flows to the first cell body 2131 through the second cell body 2132, so that the first cell body 2131 is used to store the waste liquid of the detection cell 210. The cover 215 is used to match the first cell body 2131 and the second cell body 2132. The cover 215 is installed at one end of the housing 110 away from the front cell 211, so that the first cell body 2131, the second cell body 2132 and the cover 215 are enclosed to form the rear cell 213. At this time, the second cell wall of the rear cell 213 in the above embodiment is equivalent to the cover 215 of this embodiment. The cover member 215 includes a cover body 216 and abutment 217. The waste liquid flowing from the front pool 211 is located between the first pool body 2131, the second pool body 2132 and the cover body 216. One end of the abutment 217 is connected to the cover body 216, and the other end of the abutment 217 abuts against the microporous sheet 310 to make the position of the microporous sheet 310 relatively fixed, thereby improving the stability of the microporous sheet 310.

[0052] In one embodiment, the first pool body 2131 and the covering member 215 are enclosed to form a first cavity, the second pool body 2132 and the covering member 215 are enclosed to form a second cavity, the abutment member 217 is arranged in the second cavity, the first cavity is located on the side of the second cavity away from the direction of gravity, the first pool body 2131 includes a first surface 213a connected to the second pool body 2132, and the angle between the first surface 213a and the direction of gravity is between 15°-75°.

[0053] Specifically, the first cell body 2131 and the cover 215 are enclosed to form a first cavity, and the first cavity is used to store the waste liquid of the first test sample flowing from the front cell 211. The second cell body 2132 and the cover 215 are enclosed to form a second cavity. The position of the second cavity corresponds to the position of the microporous sheet 310. When the cover 215 is installed on the housing 110, the abutment 217 of the cover 215 is set in the second cavity. The cross-sectional area of ​​the first cell body 2131 is larger than the cross-sectional area of ​​the second cell body 2132. At the connection between the first cell body 2131 and the second cell body 2132, the first cell body 2131 includes a first surface 213a and a second surface 213b for connecting with the second cell body 2132. The first surface 213a and the second surface 213b are symmetrical along the central axis of the second cell body 2132.

[0054] The angle between the first surface 213a and the gravity direction is between 15°-75°, specifically, the angle can be 15°, 20°, 25°, 30°, 35°, 40°, 45°, 50°, 55°, 60°, 65°, 70° or 75°. The angle between the second surface 213b and the gravity direction is equal to the angle between the first surface 213a and the gravity direction, which will not be repeated here. Since the angle between the first surface 213a and the direction of gravity is between 15° and 75°, the first surface 213a and the second surface 213b are inclined surfaces, there is a slope at the bottom of the first pool body 2131, and the cross-sectional area of ​​the first pool body 2131 is greater than the cross-sectional area of ​​the second pool body 2132. When the first detection sample of the front pool 211 flows from the microporous sheet 310 to the rear pool 213, the waste liquid can reduce the violent vibration caused by the liquid breaking tension when it flows from the second pool body 2132 to the first pool body 2131, so that the stability of the first detection component when collecting signals between the front pool electrode 212 and the rear pool electrode 214 is improved, thereby improving the detection accuracy of the blood cell analyzer.

[0055] In one embodiment, the abutment member 217 is sleeved on the rear cell electrode 214, and a first end face 217a and a second end face 217b are formed in the radial direction of the abutment member 217, and a flow channel is formed between the first end face 217a and the second end face 217b. The first detection sample of the front cell 211 flows through the flow channel to the rear cell 213, and the angle between the first end face 217a and the second end face 217b is between 20° and 60°.

[0056] Specifically, the material of the abutment 217 can be an elastic material such as plastic or rubber. The abutment 217 is sleeved on the rear cell electrode 214 and is used to wrap the rear cell electrode 214 to keep the rear cell electrode 214 on a preset axis. The abutment 217 forms a first end face 217a and a second end face 217b in the radial direction of the rear cell electrode 214, and a flow channel is formed between the first end face 217a and the second end face 217b, so that part of the rear cell electrode 214 is exposed from the flow channel, and the first detection sample of the front cell 211 flows through the flow channel to the rear cell 213, that is, an opening is formed between the first end face 217a and the second end face 217b of the abutment 217, and the rear cell electrode 214 is partially exposed through the opening.

[0057] After long-term research, it is found that when the angle between the first end face 217a and the second end face 217b is greater than 60°, the opening between the first end face 217a and the second end face 217b is too large, resulting in a dead angle in the flow channel, and the liquid cannot be completely filled when the first test sample flows, resulting in bubbles between the flow channels, thereby affecting the accuracy of the test results; and when the opening between the first end face 217a and the second end face 217b is too large, the capacity of the flow channel will increase, the speed of the liquid of the first test sample filling the flow channel will slow down, and the volume required for the first test sample will increase, resulting in more reagents, which is not conducive to the cost control of the reagent kit 10. When the angle between the first end face 217a and the second end face 217b is less than 20°, the opening between the first end face 217a and the second end face 217b is too small, and the liquid breaking tension when the first test sample flows from the front pool 211 to the flow channel is large, which makes it easy for bubbles to accumulate between the flow channels, affecting the first detection component to collect conductive signals and reducing the accuracy of the test results. Therefore, the angle between the first end face 217a and the second end face 217b of the reagent kit 10 of this embodiment is in the range of 20°-60°. Specifically, the angle between the first end face 217a and the second end face 217b can be 20°, 22°, 24°, 26°, 28°, 30°, 32°, 34°, 36°, 38°, 40, 42°, 44°, 46°, 48°, 50°, 52°, 54°, 56°, 58° or 60° to reduce the influence of bubbles on the flow channel and improve the detection accuracy of the blood cell analyzer.

[0058] In one embodiment, the cover body 216 is provided with a third opening at a position corresponding to the abutment 217, and a circular ring 2171 is provided at one end of the abutment 217 close to the microporous sheet 310. One end of the rear cell electrode 214 is exposed to the side wall of the rear cell 213 through the third opening, and the other end of the rear cell electrode 214 abuts against the circular ring 2171, so that when the test sample moves from the front cell 211 to the rear cell 213, the test sample contacts the rear cell electrode 214 through the hole in the middle of the circular ring 2171, so that when the rear cell electrode 214 is connected to the power supply component of the blood cell analyzer, it conducts electricity to the test sample, and the blood cell analyzer counts and detects the first test sample passing through the microporous sheet 310. In addition, the abutment member 217 abuts against the microporous sheet 310 through the annular body 2171, so as to make the detection sample conductive while pressing the microporous sheet 310 against the position of the first opening of the front pool 211, so that the position of the microporous sheet 310 is relatively fixed, reducing the impact force of the first detection sample flowing from the front pool 211 to the rear pool 213 on the microporous sheet 310, thereby improving the detection stability of the reagent kit 10.

[0059] And / or, the detection pool 210 also includes a seal (not shown) for sealing the microporous sheet 310, a second opening is provided on the side wall of the front pool 211 close to the rear pool 213, the microporous sheet 310 is provided corresponding to the second opening, and the seal is located between the microporous sheet 310 and the front pool 211.

[0060] Specifically, a second opening is provided on the side wall of the front pool 211 close to the rear pool 213, and a seal is located between the microporous sheet 310 and the side wall of the front pool 211. The seal is used to seal the microporous sheet 310 to ensure the sealing performance between the microporous sheet 310 and the second opening, so that the first detection sample can only flow from the front pool 211 to the rear pool 213 through the micropores on the microporous sheet 310, and the stability of the first detection component when collecting signals between the front pool electrode 212 and the rear pool electrode 214 is improved.

[0061] In an optional embodiment, the rear cell electrode 214 is a columnar electrode, and the central axis of the abutment 217, the central axis of the rear cell electrode 214 and the central axis of the microporous sheet 310 are all on the same straight line, that is, the abutment 217, the rear cell electrode 214 and the microporous sheet 310 are coaxially arranged to press the microporous sheet 310 against the first opening of the front cell 211, thereby further improving the stability of the microporous sheet 310.

[0062] In one embodiment, the test kit 10 includes a connection port 313 and a pressure chamber 311, the pressure chamber 311 is connected to the rear cell 213, the connection port 313 is connected to the pressure chamber 311, and the connection port 313, the other end of the front cell electrode 212 and the other end of the rear cell electrode 214 are all exposed on the same side of the shell 110.

[0063] Specifically, the pressure chamber 311 is used to provide driving power for the first test sample in the front pool 211, so that the first test sample flows from the front pool 211 to the back pool 213 through the microporous sheet 310 under pressure, so as to perform impedance detection on the first test sample of the reagent kit 10. One end of the connection port 313 is connected to the pressure source of the blood cell analyzer, and the other end of the connection port 313 is connected to the pressure chamber 311. The connection port 313 is used to provide pressure to the pressure chamber 311 when the pressure source is turned on. In an optional embodiment, the pressure source of the blood cell analyzer is a negative pressure source, and the negative pressure source is turned on. The connection port 313 is used to adjust the pressure environment of the pressure chamber 311 and the back pool 213 to a negative pressure environment, so that the first test sample in the front pool 211 flows to the back pool 213 driven by the negative pressure.

[0064] In the embodiment of the present application, the connection port 313, the other end of the front cell electrode 212 and the other end of the rear cell electrode 214 are all exposed on the same side of the shell 110. For example, the connection port 313, the other end of the front cell electrode 212 and the other end of the rear cell electrode 214 are all exposed at any position of the second side wall, the third side wall and the fourth side wall of the rear cell 213, so that when the blood cell analyzer is docked with the reagent kit 10, the blood cell analyzer only needs to be connected to the shell 110 in one direction, which facilitates the alignment connection between the reagent kit 10 and the blood cell analyzer, improves the alignment accuracy of the reagent kit 10, and further improves the connection stability between the reagent kit 10 and the blood cell analyzer.

[0065] Furthermore, the pressure chamber 311 is arranged on the side of the rear pool 213 away from the rear pool electrode 214, and the pressure chamber 311 and the rear pool 213 are arranged in sequence in the direction of gravity. The pressure chamber 311 does not occupy the area of ​​the reagent kit 10 in the length direction, and the structure is streamlined, further reducing the volume of the reagent kit 10.

[0066] Furthermore, the reagent kit 10 also includes a baffle 312 , which is located between the pressure chamber 311 and the rear pool 213 . The pressure chamber 311 is connected to the rear pool 213 through a fourth opening 314 on the baffle 312 , and the fourth opening 314 deviates from the central axis of the rear pool 213 .

[0067] Specifically, the pressure chamber 311 and the rear pool 213 are separated by the baffle 312, and the baffle 312 is provided with a fourth opening 314. The pressure chamber 311 is connected to the rear pool 213 through the fourth opening 314, so that the pressure change of the pressure chamber 311 can be transmitted to the rear pool 213 through the fourth opening 314. The fourth opening 314 of the baffle 312 deviates from the central axis of the rear pool 213, and the central axis of the rear pool 213 is represented as the central axis when facing the first pool wall or the second pool wall (i.e., the cover 215) of the rear pool 213. At this time, the fourth opening 314 can be located on the left and right sides of the central axis of the rear pool 213, that is, the fourth opening 314 can be located on the side of the central axis of the rear pool 213 that is biased towards the third pool wall (left side) or on the side of the central axis of the rear pool 213 that is biased towards the fourth pool wall (right side).

[0068] Exemplarily, the fourth opening 314 is located on the left side of the central axis of the rear pool 213. After the test of the reagent kit 10 is completed, the rear pool 213 of the reagent kit 10 stores a large amount of waste liquid generated during the test. If the reagent kit 10 is tilted to the right due to external force during transportation, the liquid level of the waste liquid in the rear pool 213 is below the plane where the fourth opening 314 is located, or the waste liquid in the rear pool 213 is slightly higher than the plane where the fourth opening 314 is located and a small amount overflows into the pressure chamber 311, and the tilting of the reagent kit 10 has little effect on the rear pool 213; when the reagent kit 10 tilts to the left, since the pressure chamber 311 and the rear pool 213 are separated by the baffle 312, the waste liquid in the rear pool 213 slowly flows to the pressure chamber 311 through the fourth opening 314, and the rising speed of the waste liquid surface of the pressure chamber 311 is relatively slow, and the tilting of the reagent kit 10 has little effect on the rear pool 213. The same is true when the fourth opening 314 is located on the right side of the central axis of the rear pool 213, which will not be repeated here.

[0069] In the embodiment of the present application, the baffle 312 is located between the pressure chamber 311 and the rear pool 213. The pressure chamber 311 is connected to the rear pool 213 through the fourth opening 314 on the baffle 312. The fourth opening 314 deviates from the central axis of the rear pool 213. After the detection is completed, if the reagent kit 10 is tipped over or other accidents occur, the waste liquid in the rear pool 213 needs to flow slowly to the pressure chamber 311 through the fourth opening 314. The rising speed of the waste liquid level in the pressure chamber 311 is relatively slow, so that the user can adjust the tipping state of the reagent kit 10 in time, reduce the possibility of leakage of the reagent kit 10, and improve the reliability of the reagent kit 10.

[0070] In one embodiment, the detection pool 210 is located at one end of the housing 110, and the reagent kit 10 is connected to a blood cell analyzer, which includes a power supply assembly (not shown) and a negative pressure source (not shown), and the power supply assembly is used to connect with the front pool electrode 212 and the rear pool electrode 214, and to conduct electricity for the detection sample between the front pool electrode 212 and the rear pool electrode 214, so that the blood cell analyzer can count and detect the first detection sample flowing through the microporous sheet 310. The negative pressure source is used to connect with the connection port 313 to provide a negative pressure environment for the pressure chamber 311 and the rear pool 213, so that the first detection sample in the front pool 211 can flow through the microporous sheet 310 and flow to the rear pool 213 under the drive of the negative pressure.

[0071] Since the connection port 313, the other end of the front cell electrode 212 and the other end of the rear cell electrode 214 are all exposed on the same side wall of the rear cell 213, the reagent kit 10 of this embodiment is connected to the blood cell analyzer only through one end face, which makes it easy for the blood cell analyzer to align with the reagent kit 10 on the same side, thereby improving the alignment accuracy of the reagent kit 10.

[0072] In one embodiment, the non-detection pool of the reagent kit 10 may include a diluent pool 412 , a hemolytic agent pool 413 , a pre-dilution pool 414 , a cleaning pool 415 , a pipette tip placement area 416 , and a sample pool 417 .

[0073] Specifically, the diluent pool 412 is used to store diluent, the hemolytic agent pool 413 is used to store hemolytic agent, the pre-dilution pool 414 is used as a place for pre-diluting samples and diluents, the cleaning pool 415 is used to store cleaning liquid, the pipette tip placement area 416 is used to insert the pipette tip, and the sample pool 417 is used to store the sample to be tested. The detection pool 210, the diluent pool 412, the hemolytic agent pool 413, the pre-dilution pool 414, the cleaning pool 415, the pipette tip placement area 416 and the sample pool 417 are respectively arranged in parallel along the length direction of the reagent box 10. The various pools of the reagent box 10 are arranged linearly, so that the pipetting component of the blood cell analyzer can perform pipetting operations between the various pools through one-dimensional movement. The blood cell analyzer does not need to set moving tracks in multiple directions, which further reduces the volume of the blood cell analyzer, is conducive to miniaturization of the blood cell analyzer, and improves the portability of the blood cell analyzer.

[0074] See also Figure 6 , Figure 6 FIG. 1 is a schematic diagram of the structure of an embodiment of a blood cell analyzer provided by the present application. Figure 6 As shown, in one embodiment, the blood cell analyzer includes a detection seat 20.

[0075] Specifically, the detection seat 20 is used to install the reagent kit 10 of any of the above embodiments; the detection seat also includes a power supply component, which is used to abut against the front cell electrode 212 and the rear cell electrode 214, and the power supply component is used to provide a detection current for the reagent kit 10, so that the blood cell analyzer can detect the sample to be tested in the reagent kit 10. At this time, when the reagent kit 10 is installed on the detection seat, the reagent kit 10 can be directly placed in an installation position on the detection seat, and the power supply component is fixed near the installation position to provide a detection current for the reagent kit 10 when it abuts against the front cell electrode 212 and the rear cell electrode 214. At this time, the reagent kit 10 has an installation direction, which corresponds to the installation position of the detection seat, for example, Figure 6 As shown, the installation position of the detection seat is located on one side of the shell 110 of the detection seat. When the user installs the reagent kit 10 on the detection seat, the user needs to move the reagent kit 10 to the installation position along a preset direction, which is the installation direction, to achieve electrical connection between the reagent kit 10 and the blood cell analyzer.

[0076] See also Figure 7 , Figure 7 FIG. 1 is a schematic diagram of the structure of another embodiment of the blood cell analyzer provided by the present application. Figure 7As shown, in another embodiment, the blood cell analyzer includes a detection seat 20 and a power supply assembly. The detection seat is used to receive any of the above test kits 10, and the detection seat is also used to move the test kit 10 to the detection position; the power supply assembly is used to dock with the front cell electrode 212 and the rear cell electrode 214 at the detection position, and the power supply assembly is used to provide a detection current for the test kit 10, so that the blood cell analyzer detects the sample to be tested in the test kit 10.

[0077] Specifically, the detection seat can be provided with a receiving position and a detection position. When the detection seat is located at the receiving position, it is used to receive the reagent kit 10. The detection seat is also used to move the reagent kit 10 from the receiving position to the detection position. The position of the power supply assembly is close to the detection position, so that the power supply assembly docks with the front pool electrode 212 and the rear pool electrode 214 at the detection position, that is, the blood cell analyzer of this embodiment needs to move the reagent kit 10 to the detection position and then dock with the power supply assembly. At this time, the detection seat has a moving direction when moving the reagent kit 10. The reagent kit 10 moves to the detection position along the moving direction and docks with the power supply assembly to achieve electrical connection between the reagent kit 10 and the blood cell analyzer.

[0078] Optionally, the connection port 313, the front cell electrode 212 and the rear cell electrode 214 of the reagent box 10 are all exposed on the same side of the housing 110. When the detection seat 20 moves or installs the reagent box 10, it is only necessary to connect one side of the reagent box 10 to the power supply component or the pressure source, thereby realizing the electrical connection between the reagent box 10 and the blood cell analyzer and the connection between the reagent box 10 and the pressure source. The blood cell analyzer realizes impedance detection and / or optical detection of the reagent box 10 by controlling the power supply state of the power supply component, the on / off state of the pressure source, etc.

[0079] Optionally, the shell 110 includes a first side surface and a second side surface, and the first side surface and the second side surface are arranged along the installation direction or the moving direction of the reagent box 10. The second side surface is located in front of the first side surface in the installation direction or the moving direction, and the front cell electrode 212 and the rear cell electrode 214 are both exposed on the second side surface of the shell 110.

[0080] Since the second side surface is located in front of the first side surface in the installation direction, when the user installs the test kit 10 on the detection seat, the power supply assembly can be abutted against the front cell electrode 212 and the rear cell electrode 214 when the test kit 10 and the test seat are in place; or, when the test seat moves the test kit 10, since the second side surface is located in front of the first side surface in the installation direction, the test seat stops moving when the power supply assembly and the test kit 10 are docked, so that the test seat can dock the power supply assembly with the electrode while the position is moved. The method is simple and easy to implement.

[0081] In the embodiment of the present application, the front cell electrode 212 and the rear cell electrode 214 are both exposed on the second side of the shell 110. The method of electrically connecting the test kit 10 to the power supply assembly is simple and convenient, which reduces the possibility of failures such as inability to detect due to inaccurate docking between the test kit 10 and the blood cell analyzer, and improves the electrical connection stability between the test kit 10 and the blood cell analyzer.

[0082] Optionally, the blood cell analyzer further comprises a pipetting component, which is used to perform pipetting operations on the non-detection pools and the detection pool 210 of the reagent kit 10 to add the first detection sample and / or the second detection sample into the detection pool 210 for detection.

[0083] Specifically, after the test base 20 receives the test kit 10 and is connected to the test kit 10, the pipette assembly moves to the pipette head placement area 416 of the test kit 10 and inserts the pipette head, and the pipette assembly moves to the diluent pool 412 of the test kit 10 and sucks the diluent in the diluent pool 412 through the pipette head to add the diluent to the pre-dilution pool 414 and the test pool 210. The sample pool 417 of the test kit 10 stores the sample to be tested, and the pipette assembly transfers the sample to be tested stored in the sample pool 417 to the pre-dilution pool 414 to obtain the pre-diluted sample to be tested through the pre-dilution pool 414. The pipette assembly sucks the pre-diluted sample to be tested in the pre-dilution pool 414 through the pipette head, and adds the pre-diluted sample to be tested to the front pool 211 of the test pool 210 to obtain the first test sample diluted to a preset multiple. The blood cell analyzer controls the pressure source to start, and the first test sample in the front pool 211 flows to the rear pool 213 through the micropores of the microporous sheet 310 under the action of pressure. A pulse is formed when the first test sample passes through the micropores. The blood cell analyzer obtains the test data of the sample to be tested by obtaining the pulse signal of the microporous sheet 310 to complete the detection of the first test sample.

[0084] Furthermore, the pipetting component is also used to transfer the first test sample in the front pool 211 to the non-detection pool of the test kit 10 in response to the completion of the detection of the first test sample, so that the pipetting component can continue to add the second test sample to the front pool 211 and detect the second test sample to achieve reuse of the detection pool 210.

[0085] Optionally, the blood cell analyzer includes a first detection component for performing impedance detection and a second detection component for performing optical detection.

[0086] After the pipetting component prepares the first test sample and the second test sample through the pipetting operation, the blood cell analyzer can perform impedance detection on the first test sample through the first detection component, and perform optical detection on the second test sample through the second detection component; or, the blood cell analyzer can perform optical detection on the first test sample through the second detection component, and perform impedance detection on the second test sample through the first detection component; or, the blood cell analyzer can also perform impedance detection on the first test sample through the first detection component, perform colorimetric detection on the second test sample through the second detection component, and then continue to perform impedance detection on the second test sample through the first detection component to obtain detection data of multiple blood cell parameters.

[0087] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A kit, characterized in that: include: case; A detection pool, arranged in the housing, comprising a front pool and a rear pool, wherein the rear pool is arranged on one side of the front pool; A microporous sheet, located between the front pool and the rear pool, the microporous sheet is provided with micropores, and the front pool and the rear pool are connected through the micropores; A front cell electrode and a rear cell electrode, one end of the rear cell electrode is located in the rear cell, one end of the front cell electrode is located in the front cell, the other end of one of the front cell electrode and the rear cell electrode extends over the microporous sheet to the side where the other electrode is located, and is exposed on the same side of the shell as the other end of the other electrode.

2. The kit according to claim 1, characterized in that One end of the front cell electrode passes over the microporous sheet and is exposed on the same side of the shell together with the other end of the rear cell electrode.

3. The kit according to claim 1 or 2, characterized in that The front cell electrode and the rear cell electrode have the same length protruding from the shell.

4. The kit according to claim 1 or 2, characterized in that The rear cell electrode and the front cell electrode are arranged along the gravity direction of the reagent box.

5. The kit according to claim 1 or 2, characterized in that The reagent box comprises a connection port and a pressure chamber, the pressure chamber is communicated with the rear cell, the connection port is connected to the pressure chamber, and the connection port, the front cell electrode and one end of the rear cell electrode are all exposed on the same side of the shell.

6. The kit according to claim 5, characterized in that The pressure chamber and the rear pool are arranged along the gravity direction of the reagent box.

7. The kit according to claim 2, characterized in that A first opening is provided at the bottom of the forecell, one end of the forecell electrode extends to a position of the forecell corresponding to the first opening, and the other end of the forecell electrode passes over the microporous sheet.

8. The kit according to claim 1, characterized in that The rear cell includes a first cell body, a second cell body and a cover member, wherein the first cell body is connected to the second cell body, and the cover member is installed at one end of the shell close to the rear cell electrode, and the first cell body, the second cell body and the cover member are enclosed to form the rear cell, and the cover member includes a cover body and a butt piece, one end of the butt piece is connected to the cover body, and the other end of the butt piece is butt-jointed with the microporous sheet.

9. A blood cell analyzer, characterized in that: include: A detection seat, used for installing the test kit according to any one of claims 1 to 8; The detection seat includes a power supply component, which is used to abut the front cell electrode and the rear cell electrode, and the power supply component is used to provide a detection current for the reagent kit so that the blood cell analyzer can detect the sample to be tested in the reagent kit.

10. A blood cell analyzer, characterized in that: include: A detection seat, used to receive the test kit according to any one of claims 1 to 8, and the detection seat is also used to move the test kit to a detection position; A power supply component is used to connect with the front cell electrode and the rear cell electrode at the detection position, and the power supply component is used to provide a detection current for the reagent kit so that the blood cell analyzer can detect the sample to be tested in the reagent kit.

11. The blood cell analyzer according to claim 9 or 10, characterized in that: The shell includes a first side surface and a second side surface, the first side surface and the second side surface are arranged along the installation direction or the moving direction of the reagent box, the second side surface is located in front of the first side surface, and the front cell electrode and the rear cell electrode are both exposed on the second side surface of the shell.

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