Traditional Chinese veterinary medicine composition for up-regulating intestinal barrier regulatory factors and application of traditional Chinese veterinary medicine composition

Abstract

The invention discloses a traditional Chinese veterinary medicine composition for up-regulating intestinal barrier regulatory factors. The traditional Chinese veterinary medicine composition is prepared from the following components: sanguisorba officinalis, pericarpium citri reticulatae, pericarpium granati and tea powder. The weight part ratio of the sanguisorba officinalis, the pericarpium citri reticulatae, the pericarpium granati and the tea powder is (5-10): (5-10): (1-5): (1-5). The traditional Chinese veterinary medicine composition is wide in medicinal material source, low in cost, free of toxic and side effects and free of drug resistance; through reasonable compatibility of the components, expression of mucous membrane tight junction protein and mucoprotein of animal intestinal tracts in the weaning period can be effectively promoted, so that the function of the intestinal mucous membrane barrier is remarkably improved, and the diarrhea rate and the death rate of animals in the weaning period are reduced. The traditional Chinese veterinary medicine composition is simple in preparation method and convenient to use, can become a potential medicine for preventing and treating diarrhea of weaned animals, and particularly has a cost advantage and a wide application prospect in the fields of green breeding and antibiotic substitution.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese veterinary medicines, and in particular to a traditional Chinese veterinary medicine composition for upregulating intestinal barrier regulatory factors, a preparation method thereof, and application thereof in weaning animal breeding. Background Art

[0002] During the weaning period, animals undergo a transition from breast milk or milk substitutes to solid feed. The physiological and immune changes that accompany this process significantly reduce the adaptability of weaned animals, especially rabbits, piglets, and calves, to the external environment, making them more susceptible to digestive tract diseases such as diarrhea. Studies have shown that the mucosal barrier is a key line of defense for maintaining intestinal health and defending against exogenous pathogens, and its dysfunction is closely related to the occurrence of diarrhea in weaned animals.

[0003] The intestinal mucosal barrier is mainly composed of intestinal epithelial cells, intestinal mucus layer, intestinal microbiota and immune cells, and has the triple functions of physical barrier, immune defense and biological barrier. During the weaning process, the intestinal microbiota of animals changes, and the integrity of the intestinal barrier may be damaged, which is manifested by damaged tight junctions of epithelial cells, reduced mucus layer thickness, weakened immune response function, etc., which leads to increased intestinal susceptibility. Pathogenic microorganisms such as Escherichia coli and Salmonella can easily invade the intestine and induce diarrhea.

[0004] In addition, the weaning process is often accompanied by stress reactions. Changes in feed, fluctuations in environmental temperature, transportation and other factors may further aggravate the damage to the mucosal barrier, leading to increased intestinal permeability and intensified inflammatory responses. Studies have found that there is a direct correlation between intestinal inflammatory responses and disorders of the mucosal barrier function. The activation of intestinal immune cells such as macrophages and T lymphocytes can lead to local inflammatory responses, further aggravating intestinal dysfunction. Therefore, maintaining the health of the intestinal mucosal barrier, especially during the weaning period, is the key to preventing and treating diarrhea.

[0005] From the perspective of veterinary drug research and development, improving mucosal barrier function has become an important research direction. By enhancing the intestinal barrier function, regulating the balance of intestinal microecology, and inhibiting the overgrowth of harmful bacteria, the incidence of diarrhea in weaned animals can be effectively reduced, and their growth performance and immune health can be improved. Therefore, the development of veterinary drug products that can promote intestinal barrier repair, improve the intestinal microenvironment, and reduce stress response has important practical significance for the health of weaned animals. Summary of the invention

[0006] The purpose of the present invention is to solve the problem of diarrhea in weaned animals existing in the prior art, and to provide a Chinese veterinary medicine composition and application for upregulating intestinal barrier regulatory factors.

[0007] The technical solution adopted by the present invention is as follows: a Chinese veterinary medicine composition for upregulating intestinal barrier regulatory factors, composed of the following components: sanguisorba officinalis, tangerine peel, pomegranate peel and tea powder; the weight ratio of the sanguisorba officinalis, tangerine peel, pomegranate peel and tea powder is (5-10): (5-10): (1-5): (1-5).

[0008] Preferably, the weight ratio of the sanguisorba officinalis, tangerine peel, pomegranate peel and tea powder is 10:10:5:5.

[0009] The present invention also provides a method for preparing the above-mentioned Chinese veterinary drug composition, comprising the following steps:

[0010] S1. Weigh dandelion, tangerine peel, pomegranate peel and tea powder according to weight ratio, and grind them into coarse powder;

[0011] S2, add 80% ethanol to reflux extraction and collect the extract, repeat twice, combine the extracts and concentrate under reduced pressure, concentrate until there is no alcohol taste, spray dry to powder, and obtain extract 1; take the residue, add 10 times the amount of water to reflux extraction and collect the extract, repeat twice, combine the extracts and concentrate under reduced pressure and spray dry to powder, and obtain extract 2;

[0012] S3. Evenly mix the extract 1 and the extract 2 to obtain the Chinese veterinary medicine composition.

[0013] The present invention also provides the use of the Chinese veterinary medicine composition in animal breeding, wherein the Chinese veterinary medicine composition is mixed with feed and then fed to weaning animals.

[0014] Preferably, the animals include rabbits and pigs.

[0015] Preferably, the concentration of the Chinese veterinary medicine composition added to the feed is 300g / ton to 500g / ton.

[0016] The beneficial effects of the present invention are:

[0017] 1) The present invention provides a Chinese veterinary medicine composition for up-regulating intestinal barrier regulatory factors of weaned animals, which is composed of radix sanguisorbae, tangerine peel, pomegranate peel and tea powder. The Chinese veterinary medicine composition has a wide range of medicinal materials, low cost, no toxic side effects, and no drug resistance; through the reasonable combination of each component, it can effectively promote the expression of tight junction proteins and mucins in the intestinal mucosa of weaned animals, while increasing the height of intestinal villi and the thickness of the base layer, thereby significantly improving the function of the intestinal mucosal barrier and reducing the diarrhea rate and mortality rate of weaned animals.

[0018] 2) The preparation method of the Chinese veterinary medicine composition of the present invention is simple and easy to use. In actual application, the Chinese veterinary medicine composition is mixed with feed or water and then fed to weaned animals, which can effectively enhance the function of the intestinal mucosal barrier of the animals and can become a potential drug for the prevention and treatment of diarrhea in weaned animals. In particular, it has cost advantages and broad application prospects in the fields of green breeding and antibiotic substitution. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The invention discloses the effect of the Chinese veterinary medicine composition on the intestinal barrier of weaned rabbits.

[0020] Figure 2 This is a pathological section of the intestinal tract of a weaned rabbit in Example 2.

[0021] Figure 3 The invention discloses an effect of the Chinese veterinary medicine composition on the intestinal barrier of weaned piglets.

[0022] Figure 4 This is a pathological section of the intestine of a weaned piglet in Example 3. DETAILED DESCRIPTION

[0023] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0024] Preparation of the veterinary drug composition in Example 1

[0025] Weigh sanguisorba officinalis, dried orange peel, pomegranate peel and tea powder (10:10:5:5) by weight, combine and grind into coarse powder, add 10 times the amount of 80% ethanol to reflux extraction and collect the extract, repeat twice and combine the extracts and reduce pressure concentration, concentrate until there is no alcohol taste, spray dry to powder, and obtain extract 1. Take the residue, add 10 times the amount of water to reflux extraction and collect the extract, repeat twice and combine the extracts and reduce pressure concentration and spray dry to powder, and obtain extract 2. Mix extract 1 and extract 2 evenly to obtain a Chinese veterinary drug composition.

[0026] Effect of the veterinary drug composition in Example 2 on the intestinal barrier of weaned rabbits

[0027] The Chinese veterinary medicine composition in this example is prepared by Example 1.

[0028] 1. Experimental animals: 60 weaned rabbits of similar weight were randomly divided into three groups, 20 in each group. Control group: fed with basic feed without adding Chinese veterinary medicine composition; low-dose group of Chinese veterinary medicine composition: fed with basic feed with Chinese veterinary medicine composition added, 300g / ton; high-dose group of Chinese veterinary medicine composition: fed with basic feed with Chinese veterinary medicine composition added, 500g / ton.

[0029] 2. Feeding and management: All animals were raised under the same environmental conditions, with adequate drinking water and free access to food. The experimental period lasted for 28 days. During the experiment, the weight gain, diarrhea rate and mortality of animals in each group were counted, and all animals were killed on the 29th day. Jejunal tissues were collected and the expression levels of intestinal tight junction proteins and mucin mRNA were detected by PCR.

[0030] 3. Experimental results: Figure 1 It can be seen that the Chinese veterinary medicine composition significantly improved the weight gain of weaned rabbits and reduced the number of diarrhea and deaths. Pathological sections of the intestine ( Figure 2 ) showed that the Chinese veterinary medicine composition significantly increased the height of intestinal villi. From the expression of mucosal tight junction proteins and mucin genes ( Figure 1 ), the Chinese veterinary medicine combination showed a very strong promoting effect, which became a key factor in improving the intestinal health of baby rabbits.

[0031] Effect of the veterinary drug composition in Example 3 on the intestinal barrier of weaned piglets

[0032] The Chinese veterinary medicine composition in this example is prepared by Example 1.

[0033] 1. Experimental animals: 30 weaned piglets of similar weight were randomly divided into three groups, with 10 piglets in each group. Control group: fed with basic feed without adding Chinese veterinary medicine composition; low-dose group of Chinese veterinary medicine composition: fed with basic feed with Chinese veterinary medicine composition added, 300g / ton; high-dose group of Chinese veterinary medicine composition: fed with basic feed with Chinese veterinary medicine composition added, 500g / ton.

[0034] 2. Feeding and management: All animals were raised under the same environmental conditions, with adequate drinking water and free access to food. The experimental period lasted for 28 days. During the experiment, the weight gain, diarrhea rate and mortality of animals in each group were counted, and all animals were killed on the 29th day. Jejunal tissues were collected and the expression levels of intestinal tight junction proteins and mucin mRNA were detected by PCR.

[0035] 3. Experimental results: Figure 4 The intestinal sections of pigs fed with the Chinese veterinary medicine composition showed that the height, width and thickness of the intestinal villi of the pigs fed with the Chinese veterinary medicine composition were significantly better than those of the control group. Figure 3 It can be seen that the Chinese veterinary medicine composition significantly promoted the expression of mucosal tight junction proteins and mucins in weaned piglets, improved the intestinal health of piglets, thereby effectively improving the weight gain of weaned piglets and reducing diarrhea and mortality.

[0036] In summary, the Chinese veterinary medicine composition prepared by the present invention is mixed with the basic feed for feeding weaned animals, which can significantly promote the expression of tight junction proteins and mucins in the intestinal mucosa, thereby effectively improving the intestinal health of animals and reducing diarrhea and mortality of animals during the weaning period.

[0037] The specification and drawings of the present invention are considered to be illustrative rather than restrictive. On the basis of the present invention, those skilled in the art can make some substitutions and modifications to some of the technical features according to the disclosed technical content without creative labor, all of which are within the protection scope of the present invention.

Claims

1. A Chinese veterinary medicine composition for upregulating intestinal barrier regulatory factors, characterized in that: The invention is composed of the following components: sanguisorba officinalis, tangerine peel, pomegranate peel and tea powder; the weight ratio of the sanguisorba officinalis, tangerine peel, pomegranate peel and tea powder is (5-10): (5-10): (1-5): (1-5).

2. The Chinese veterinary medicine composition according to claim 1, characterized in that: The weight ratio of the sanguisorba officinalis, tangerine peel, pomegranate peel and tea powder is 10:10:5:

5.

3. The method for preparing the Chinese veterinary drug composition according to claim 1 or 2, characterized in that: The following steps are involved: S1. Weigh dandelion, tangerine peel, pomegranate peel and tea powder according to weight ratio, and grind them into coarse powder; S2, add 80% ethanol to reflux extraction and collect the extract, repeat twice, combine the extracts and concentrate under reduced pressure, concentrate until there is no alcohol taste, spray dry to powder, and obtain extract 1; take the residue, add 10 times the amount of water to reflux extraction and collect the extract, repeat twice, combine the extracts and concentrate under reduced pressure and spray dry to powder, and obtain extract 2; S3. Evenly mix the extract 1 and the extract 2 to obtain the Chinese veterinary medicine composition.

4. The use of the Chinese veterinary medicine composition according to claim 1 or 2 in animal breeding, characterized in that: The Chinese veterinary medicine composition is mixed with feed and then fed to weaning animals.

5. The use according to claim 4, characterized in that: The animals include rabbits and pigs.

6. The use according to claim 4, characterized in that: The concentration of the Chinese veterinary medicine composition added to the feed is 300g / ton to 500g / ton.

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