Traditional Chinese medicine composition for dredging channels and benefiting intelligence

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and particularly discloses a traditional Chinese medicine composition for promoting blood circulation, dredging vessels, tonifying kidney and promoting intelligence and a preparation method thereof, and the traditional Chinese medicine composition is prepared from red peony root, ligusticum wallichii, prepared rhizome of rehmannia, dogwood, semen brassicae, polygala tenuifolia, rhizoma acori graminei, angelica sinensis, poria cocos and other raw medicinal materials. The whole formula adopts the treatment method of promoting blood circulation to remove meridian obstruction, tonifying kidney and promoting intelligence, and especially for senile vascular dementia, the cognitive impairment of rats with vascular dementia and clinical patients can be remarkably improved. The invention further provides a preparation and a preparation method of the traditional Chinese medicine composition, and the whole process preparation method is simple and convenient and easy to popularize.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for promoting blood circulation and improving intelligence. Background Art

[0002] Dementia mainly destroys nerve cells and damages the brain, with cognitive dysfunction and memory loss as core symptoms, accompanied by varying degrees of mental and behavioral abnormalities. If not treated in time, patients may lose the ability to live independently within a few years.

[0003] Vascular dementia (VD) has a complex pathogenesis and is more common in elderly patients. It is mainly caused by ischemic stroke, hemorrhagic stroke and cerebrovascular disease that causes low perfusion of brain areas such as memory, cognition and behavior, resulting in severe cognitive dysfunction. Modern research has confirmed that hypertension, diabetes, atherosclerosis, hyperlipidemia, smoking, etc. can induce the development of the disease, and further divides vascular dementia into subcortical ischemic vascular cognitive impairment dementia, multiple cerebral infarction dementia, post-stroke dementia, mixed dementia and other types.

[0004] Western medicine treatment of vascular dementia mainly includes targeted treatment and rehabilitation for preventing and treating stroke, improving cognitive function and controlling mental and behavioral symptoms. Donepezil, galantamine and carbaladine have certain effects on improving blood cognitive function; FDA-approved memantine can delay cognitive decline in patients with mild to moderate vascular dementia, but withdrawal and adverse reactions are frequent; in addition, butylphthalide and cilostazol also have certain protection for cognitive function after stroke. However, the treatment of vascular dementia has not improved significantly.

[0005] According to TCM, vascular dementia belongs to the category of "dementia" and "forgetfulness". The disease is located in the brain and is closely related to the heart, liver, spleen and kidney. It is closely related to pathological factors such as dysfunction of internal organs, deficiency of qi and blood, phlegm stagnation, and blood stasis. The treatment strategy mainly focuses on tonifying the kidney and replenishing essence, promoting blood circulation and removing blood stasis, and removing phlegm and opening the mind. In recent years, studies have confirmed that TCM has unique advantages and methods in the treatment of dementia. Generally, the combination of Chinese medicine, acupuncture, massage, diet therapy and other methods can effectively improve the patient's brain function and improve the quality of life.

[0006] In recent years, the trend of population aging has become severe, and the medical and social burden brought by vascular dementia in the elderly has become one of the problems that need to be solved urgently. Summary of the invention

[0007] The invention aims to provide a Chinese medicine composition for promoting blood circulation, unblocking meridians, tonifying kidney and improving intelligence, comprising red peony root, chuanxiong, rehmannia root, cornus officinalis, white mustard seed, polygala root, calamus calamus, angelica root and tuckahoe.

[0008] A further technical problem to be solved by the present invention is to provide a pharmaceutical preparation for treating dementia, which has a therapeutic effect superior to that of a positive control drug.

[0009] In order to solve the above technical problems, the inventors firstly provide a Chinese medicine composition for promoting blood circulation, unblocking meridians, tonifying kidney and improving intelligence, comprising the following raw medicinal materials:

[0010] 8-15 parts by weight of Chinese Angelica sinensis 10-18 parts by weight of Red Peony Root 10-18 parts by weight of Chuanxiong

[0011] 20-40 parts by weight of Poria cocos 10-18 parts by weight of Rehmannia glutinosa 20-30 parts by weight of Cornus officinalis

[0012] Acorus calamus 10-18 parts by weight, Polygala tenuifolia 8-12 parts by weight, Sinapis alba 8-12 parts by weight.

[0013] Furthermore, in order to improve the efficacy and reduce adverse reactions, the inventors limit the mustard to white mustard and the rehmannia to cooked rehmannia.

[0014] Furthermore, after a large number of experiments, the inventors found that the Chinese medicine composition has better effects when it includes the following Chinese medicine components:

[0015] 12 parts by weight of Chinese Angelica sinensis 15 parts by weight of Radix Paeoniae Rubra 15 parts by weight of Rhizoma Chuanxiong

[0016] 30 parts by weight of Poria cocos 15 parts by weight of Rehmannia glutinosa 24 parts by weight of Cornus officinalis

[0017] 15 parts by weight of Acorus calamus, 9 parts by weight of Polygala tenuifolia, 9 parts by weight of Sinapis alba.

[0018] In addition, in order to provide a drug with excellent therapeutic effect, easy for patients to take, and safer and more effective, the inventor further prepares the Chinese medicine composition into a clinical oral preparation, and the drug is one or more of granules, mixtures, oral liquids, pills, capsules or tablets.

[0019] At the same time, the present invention also provides a method for preparing the Chinese medicine composition preparation:

[0020] In a preferred embodiment, the preparation method is optimized as follows: the above-mentioned Chinese medicinal materials are crushed and sieved; Poria cocos, Rehmannia glutinosa, Acorus calamus, and Polygala tenuifolia are taken, and ultrasonic extraction is performed with water to obtain an extract, and the residue is reserved; the other remaining medicinal materials and residue are mixed, and a composite enzyme is added for enzymatic hydrolysis to obtain an enzyme ultrasonic hydrolyzate, wherein the composite enzyme in the enzymatic hydrolysis step includes 9-15 parts by weight of citrate synthase, 4-9 parts by weight of chitinase, and 2-8 parts by weight of mannanase, the temperature of the ultrasonic extraction is 35°C to 45°C, the power of the ultrasonic extraction is 100W to 180W, and the time is 25min to 45min; the extract and the enzymatic hydrolyzate are mixed, concentrated and dried to obtain an extract, and conventional pharmaceutical excipients are added to prepare a clinically acceptable dosage form.

[0021] In another preferred embodiment, the preparation method is optimized as follows: the above-mentioned Chinese medicinal materials are crushed and sieved; Poria cocos, Rehmannia glutinosa, Acorus calamus, and Polygala tenuifolia are taken, and ultrasonic extraction is performed with water to obtain an extract, and the residue is reserved; the other remaining medicinal materials and residues are mixed, and after adding the composite enzyme, the pH value of the solution is adjusted to 5.5-6.5. In the enzymatic hydrolysis step, the composite enzyme includes 9-15 parts by weight of citrate synthase, 4-9 parts by weight of chitinase, and 2-8 parts by weight of mannanase. The temperature of the ultrasonic extraction is 35°C-45°C, the power of the ultrasonic extraction is 100W-180W, and the time is 25min-45min; the extract and the enzymatic hydrolyzate are mixed, concentrated and dried to obtain an extract, and conventional pharmaceutical excipients are added to prepare a clinically acceptable dosage form.

[0022] Based on the results of pharmacological experiments, another object of the present invention is to provide the use of the above-mentioned Chinese medicine composition in the preparation of drugs for treating and improving memory loss and cognitive dysfunction. Furthermore, the use is the use of the drug of the present invention in the preparation of drugs for treating dementia, especially in the preparation of drugs for treating vascular dementia.

[0023] The Chinese medicine composition of the present invention focuses on promoting blood circulation and unblocking meridians, tonifying the kidney and improving intelligence. The red peony root in the prescription clears away heat and cools blood, dissipates blood stasis and relieves pain. Chuanxiong promotes blood circulation and qi circulation, and the red peony root is bitter and cold. The two medicines are used together to increase the effect of promoting blood circulation and removing blood stasis, and borrow the power of qi and blood circulation to increase the effect of blood circulation. Rehmannia glutinosa nourishes the kidney and replenishes essence, and Cornus officinalis and Rehmannia glutinosa are used together to tonify the kidney and restrain the liver; white mustard seeds are added to search for collaterals and eliminate phlegm, Polygala tenuifolia strengthens the will and improves intelligence, and Acorus calamus opens the mind and improves intelligence. The five medicines are used together to play the effect of tonifying the kidney and strengthening the will. Angelica sinensis nourishes blood and activates blood circulation, and Poria strengthens the spleen, so that the biochemical activity of qi and blood is active, and when qi and blood are abundant, it is easy to circulate without stagnation. The combination of all the medicines plays the effect of promoting blood circulation and unblocking meridians, tonifying the kidney and improving intelligence.

[0024] The beneficial effects of the present invention are as follows:

[0025] (1) The Chinese medicine composition provided by the present invention has a good effect of improving memory loss and cognitive dysfunction, and can significantly improve the clinical symptoms of vascular dementia;

[0026] (2) In the composition provided by the present invention, compared with the positive control drug, the Chinese medicine composition of the present invention can achieve similar or better improvement effects, which proves that the Chinese medicine composition of the present invention has a reasonable compatibility and good coordination of the components. On the other hand, it also benefits from the reasonable preparation method of the present invention, which ensures that the effective components are efficiently extracted;

[0027] (3) The Chinese medicine components used in the present invention are not complicated, and no toxic reactions or adverse reactions were found during the study, indicating that the Chinese medicine composition of the present invention has low toxicity and few adverse reactions and can be promoted and applied. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1The effect of the composition of the present invention on the AchE value in the serum of rats with vascular dementia (compared with the sham operation group, **P<0.01; compared with the model control group, ## P<0.01);

[0029] Figure 2 The effect of the composition of the present invention on the 5-HT value in the serum of rats with vascular dementia (compared with the sham operation group, **P<0.01; compared with the model control group, ## P<0.01);

[0030] Figure 3 The effect of the composition of the present invention on the content of TNF-α in the brain tissue of rats with vascular dementia (compared with the sham operation group, **P<0.01; compared with the model control group, ## P<0.01)

[0031] Figure 4 The effect of the composition of the present invention on the IL-1β content in brain tissue of rats with vascular dementia (compared with the sham operation group, **P<0.01; compared with the model control group, ## P<0.01)

[0032] Figure 5 The effect of the composition of the present invention on the ADL score and MMSE score of patients with vascular dementia (compared with the treatment group before treatment, ADL score **P < 0.01, MMSE score $$ P<0.01; compared with the control group before treatment, the ADL score @@ P<0.01, MMSE score ％％ P<0.01; compared with the control group after treatment, the ADL score & P<0.05, MMSE score^P<0.05;) DETAILED DESCRIPTION

[0033] The present invention is further described below by way of examples. It should be correctly understood that the examples of the present invention are only used to illustrate the present invention, rather than to limit the present invention, and those skilled in the art should be aware that the examples described herein do not limit the present invention in any way.

[0034] Example 1 Preparation of granules

[0035] Prescription: Angelica 8g, Red Peony Root 10g, Chuanxiong 10g

[0036] Poria 40g, Rehmannia glutinosa 10g, Cornus officinalis 30g

[0037] Acorus calamus 18g Polygala tenuifolia 8g White mustard seeds 12g

[0038] Preparation method:

[0039] (1) Grind the above Chinese medicinal materials, pass through a 120-mesh sieve, and set aside;

[0040] (2) Take Poria cocos, Rehmannia glutinosa, Acorus calamus, and Polygala tenuifolia, add twice the amount of water, decoct for 2 h, separate, and obtain the extract. The residue is reserved for later use;

[0041] (3) mixing other remaining medicinal materials and medicinal residues, adding a composite enzyme, wherein the composite enzyme in the enzymatic hydrolysis step includes 15 parts by weight of citrate synthase, 4 parts by weight of chitinase, and 8 parts by weight of mannanase, the temperature of the ultrasonic extraction is 35° C. to 45° C., the power of the ultrasonic extraction is 100 W to 180 W, the time is 25 min, and enzymolysis is performed to obtain an enzymatic solution;

[0042] (4) The extract and the enzymatic hydrolysate are mixed, concentrated and dried under reduced pressure at 55°C to 65°C to obtain an extract, and conventional pharmaceutical excipients are added to prepare granules.

[0043] Example 2 Preparation of granules

[0044] Prescription: Angelica 15g, Red Peony Root 18g, Chuanxiong 18g

[0045] Poria 20g, Rehmannia glutinosa 18g, Cornus officinalis 20g

[0046] Acorus calamus 10g Polygala tenuifolia 12g White mustard seeds 8g

[0047] (1) Grind the above Chinese medicinal materials, pass through a 120-mesh sieve, and set aside;

[0048] (2) Take Poria cocos, Rehmannia glutinosa, Acorus calamus, and Polygala tenuifolia, add 3 times the amount of water, boil for 1.5 h, separate, and obtain the extract. The residue is reserved for later use;

[0049] (3) Mixing other remaining medicinal materials and medicinal residues, adding complex enzymes, wherein the complex enzymes in the enzymatic hydrolysis step include 9 parts by weight of citrate synthase, 9 parts by weight of chitinase, and 2 parts by weight of mannanase, the temperature of the ultrasonic extraction is 35° C. to 45° C., the power of the ultrasonic extraction is 100 W to 180 W, the time is 45 min, and enzymolysis is performed to obtain an enzymolysis solution;

[0050] (4) The extract and the enzymatic hydrolysate are mixed, concentrated and dried under reduced pressure at 55°C to 65°C to obtain an extract, and conventional pharmaceutical excipients are added to prepare granules.

[0051] Example 3 Preparation of granules

[0052] Prescription: Angelica 12g, Red Peony Root 15g, Chuanxiong 15g

[0053] Poria 30g, Rehmannia glutinosa 15g, Cornus officinalis 24g

[0054] Acorus calamus 15g Polygala tenuifolia 9g White mustard seeds 9g

[0055] (1) Grind the above Chinese medicinal materials, pass through a 120-mesh sieve, and set aside;

[0056] (2) Take Poria cocos, Rehmannia glutinosa, Acorus calamus, and Polygala tenuifolia, add 3 times the amount of water, boil for 1.5 h, separate, and obtain the extract. The residue is reserved for later use;

[0057] (3) mixing other remaining medicinal materials and medicinal residues, adding a composite enzyme, wherein the composite enzyme in the enzymatic hydrolysis step includes 12 parts by weight of citrate synthase, 7 parts by weight of chitinase, and 5 parts by weight of mannanase, the temperature of the ultrasonic extraction is 35° C. to 45° C., the power of the ultrasonic extraction is 100 W to 180 W, the time is 45 min, and enzymolysis is performed to obtain an enzymolysis solution;

[0058] (4) The extract and the enzymatic hydrolysate are mixed, concentrated and dried under reduced pressure at 55°C to 65°C to obtain an extract, and conventional pharmaceutical excipients are added to prepare granules.

[0059] Example 4 Preparation of granules

[0060] Prescription: Angelica 10g, Red Peony Root 15g, Chuanxiong 15g

[0061] Poria 28g, Rehmannia glutinosa 15g, Cornus officinalis 27g

[0062] Acorus calamus 15g Polygala tenuifolia 10g White mustard seeds 10g

[0063] The preparation method is the same as Example 1.

[0064] Example 5 Preparation of tablets

[0065] Prescription: Angelica 10g, Red Peony Root 12g, Chuanxiong 12g

[0066] Poria 35g, Rehmannia glutinosa 13g, Cornus officinalis 28g

[0067] Acorus calamus 12g Polygala tenuifolia 10g White mustard seeds 10g

[0068] Steps (1) to (3) are the same as in Example 2, and step (4) is prepared into tablets according to a conventional preparation method.

[0069] Example 6 Preparation of mixture

[0070] Prescription: Angelica 8g, Red Peony Root 10g, Chuanxiong 18g

[0071] Poria 40g, Rehmannia glutinosa 16g, Cornus officinalis 28g

[0072] Acorus calamus 12g Polygala tenuifolia 8g White mustard seeds 10g

[0073] Steps (1) to (3) are the same as those in Example 2, and step (4) is prepared into a mixture according to a conventional preparation method.

[0074] Preparation of Comparative Example 1 Granules

[0075] Prescription: Angelica 20g, Red Peony Root 25g, Chuanxiong 5g

[0076] Poria 5g, Rehmannia glutinosa 15g, Cornus officinalis 40g

[0077] Acorus calamus 15g Polygala tenuifolia 10g White mustard seeds 8g

[0078] The preparation method is the same as Example 1.

[0079] Preparation of Comparative Example 2 Granules

[0080] Prescription: Angelica 15g, Red Peony Root 18g, Chuanxiong 15g

[0081] Poria 40g Rehmannia glutinosa 18g

[0082] Acorus calamus 18g Polygala tenuifolia 12g

[0083] The preparation method is the same as Example 1.

[0084] Preparation of Comparative Example 3 Granules

[0085] Prescription: Angelica 12g, Peony Root 18g, Corydalis 18g

[0086] Poria 30g, Rehmannia glutinosa 16g, Cornus officinalis 25g

[0087] Acorus calamus 12g Polygala tenuifolia 8g White mustard seeds 10g

[0088] The preparation method is the same as Example 1.

[0089] Preparation of Comparative Example 4 Granules

[0090] Prescription: Angelica 8g, Red Peony Root 10g, Chuanxiong 18g

[0091] Poria 25g, processed Polygonum multiflorum 15g, Chinese yam 30g

[0092] Acorus calamus 12g, cypress seed 8g, pinellia 10g

[0093] The preparation method is the same as Example 1.

[0094] Preparation of Comparative Example 5 Granules

[0095] Prescription: Angelica 8g, Red Peony Root 10g, Chuanxiong 18g

[0096] Poria 40g, Rehmannia glutinosa 16g, Cornus officinalis 28g

[0097] Acorus calamus 12g Polygala tenuifolia 8g White mustard seeds 10g

[0098] Preparation method:

[0099] (1) Grind the above Chinese medicinal materials, pass through a 120-mesh sieve, and set aside;

[0100] (2) Take Poria cocos, Rehmannia glutinosa, Acorus calamus, and Polygala tenuifolia, add 3 times the amount of water, decoct for 1.5 h, extract 3 times, separate, combine the extracts, and keep the residue for later use;

[0101] (3) Mix the remaining medicinal materials and medicinal residues, add 3 times the amount of water, perform ultrasonic extraction at a temperature of 35° C. to 45° C., a power of 100 W to 180 W, a time of 45 min, perform extraction 3 times, separate, and obtain an extract;

[0102] (4) The two extracts are mixed, concentrated and dried under reduced pressure at 55°C to 65°C to obtain an extract, and conventional pharmaceutical excipients are added to prepare granules.

[0103] Verification experiment 1 Effect of the composition of the present invention on vascular dementia rat model

[0104] 1 Experimental Materials

[0105] 1.1 Experimental animals

[0106] Healthy male SD rats (provided by the Pharmacology Center of Lunan Pharmaceutical Group) weighing 200-220 g were used for the experiment after 7 days of adaptive feeding.

[0107] 1.2 Experimental Reagents

[0108] Commercially available nimodipine tablets, granules of Example 1, granules of Example 4, granules of Comparative Example 3, and granules of Comparative Example 5.

[0109] 2 Test methods

[0110] 2.1 Modeling

[0111] The rat model was prepared by a modified bilateral carotid artery ligation method. After the experimental rats were anesthetized by intraperitoneal injection of 4% sodium pentobarbital, a 1-2 cm longitudinal incision was made on the left side of the midline of the rat's neck, and the left common carotid artery was permanently ligated, disinfected and sutured. Seven days later, the right common carotid artery of the rat was ligated in the same way. The sham operation group only underwent a longitudinal incision to separate the left and right common carotid arteries but did not ligate. Seven days after ligation, the rats were subjected to the Morris water maze test after dawn. The sham operation group rats were used as the control. The difference between the escape latency of the model group rats and the escape latency of the sham operation group rats and the ratio of the difference to the escape latency of the model group rats were calculated. The model was successfully established when the ratio was >20%.

[0112] 2.2 Grouping

[0113] The rats with successful modeling were divided into a model group, an example 1 group, an example 4 group, a comparative example 3 group, and a comparative example 5 group, with 10 rats in each group. The drugs were administered starting from 7 days after the second ligation operation. The positive control group was intragastrically administered with 10.8 mg / kg of nimodipine suspension, and the sham operation group and the model group were intragastrically administered with the same volume of normal saline once a day for 28 consecutive days. The example 1 group, the example 4 group, the comparative example 3 group, and the comparative example 5 group were intragastrically administered with the suspension of the preparation prepared in the corresponding example, and the dosage was 10.2 g / kg based on the crude drug amount.

[0114] 2.3 Index detection

[0115] 2.3.1 Morris water maze test

[0116] Before the experiment, a 4-day training period was conducted. On the 5th day, the time required for the rat to stand on the platform was recorded, which was the escape latency. If the rat failed to stand on the platform within 90 seconds, the escape latency was counted as 90 seconds. After the spatial exploration test began, the number of times the rat crossed the original platform was recorded, and the time was controlled within 90 seconds.

[0117] 2.3.2 Determination of AchE and 5-HT activity in rat serum

[0118] After the water maze experiment, blood was collected from the carotid artery, and the AchE and 5-HT levels in the serum were determined according to the kit requirements.

[0119] 2.3.3 Determination of TNF-α and IL-1β Content in Rat Brain Tissue

[0120] The rats were killed, and the brain tissue was taken and homogenized, and the contents of TNF-α and IL-1β in the brain tissue were determined according to the requirements of the kit.

[0121] 2.3.4 Data processing

[0122] SPSS 22.0 software was used for statistical analysis, and the data were expressed as “mean ± standard deviation”. One-way analysis of variance was used for analysis among multiple groups, and P < 0.05 was considered statistically significant.

[0123] 3 Test results

[0124] 3.1 Experimental results on the learning and memory ability of model rats

[0125] The results of the Morris water maze test showed that compared with the sham operation group, the model group rats had a prolonged escape latency and a significantly reduced number of crossings of the original platform position, and the difference was statistically significant (P<0.01); compared with the model group, the escape latency of Example 1 group, Example 4 group, and nimodipine group were shortened, and the number of crossings of the original platform increased, and the difference was statistically significant (P<0.05, P<0.01); while there was no significant difference between Example 5 group and the model group (P>0.05).

[0126] The specific results are shown in Table 1.

[0127] Table 1 Comparison of escape latency and number of crossing the original platform in each group of rats (n=10, )

[0128]

[0129] Note: Compared with the sham operation group, **P<0.01; compared with the model control group, ## P<0.01.

[0130] 3.2 Results of the measurement of serum AchE and 5-HT activities in model rats

[0131] The results are as follows Figure 1 , Figure 2 As shown in the figure, compared with the sham operation group, the serum AchE value of the model group rats was significantly increased, and the 5-HT value was significantly reduced, and the difference was statistically significant (P<0.05). After treatment, compared with the model group, the AchE value of Example 1 group, Example 4 group, and nimodipine group was significantly reduced, and the 5-HT value was significantly increased (P<0.05), and the difference was statistically significant (P<0.05); while the AchE value and 5-HT value of the comparative example 5 group were compared with the model group, and there was no significant difference (P>0.05).

[0132] 3.3 Results of determination of TNF-α and IL-1β contents in brain tissue of model rats

[0133] The results are as follows Figure 3 , Figure 4 As shown. It can be seen from the figure that compared with the sham operation group, the serum TNF-α and IL-1β values ​​of the model group rats were significantly increased, and the difference was statistically significant (P<0.05). After treatment, compared with the model group, the TNF-α and IL-1β values ​​of Example 1 group, Example 4 group, and nimodipine group showed different degrees of decrease (P<0.05), and the difference was statistically significant (P<0.05); while there was no significant difference in the TNF-α and IL-1β values ​​of Example 5 group compared with the model group (P>0.05).

[0134] Validation experiment 1 Effect of Chinese herbal medicine combination on patients with vascular dementia

[0135] 1. Materials and methods

[0136] 1.1 Research subjects

[0137] 14 patients with vascular dementia. Age ranged from 58 to 72 years old, with an average age of (66.23±6.35) years old; BMI index ranged from 21.6 to 25.2 kg / m 2 , average (24.06±1.65)Kg / m 2 .

[0138] 1.2 Western medicine diagnostic criteria:

[0139] ①Meet the diagnostic criteria for vascular dementia of the Neurology Branch of the Chinese Medical Association in 2002; ②The Mini-Mental State Examination ADL score is between 21 and 27 points, and the MMSE score is between 7 and 12 points.

[0140] 1.3 Exclusion criteria:

[0141] ① Those with mental illness, epilepsy, delirium, ② Those who abuse drugs or drink heavily, and other systemic diseases that can cause cognitive function and memory disorders.

[0142] 1.4 Grouping:

[0143] They were randomly divided into a control group and an experimental group, with 8 people in each group.

[0144] 1.5 Treatment

[0145] (1) Control group: 30 mg / d of commercially available nicergoline capsules, once a day for 42 consecutive days.

[0146] (2) Experimental group: 8 g of the granules prepared in Example 4 were taken at a time, 3 times a day, for 42 consecutive days.

[0147] 2. Observation indicators

[0148] Six weeks after the experiment, the two groups of patients were scored again on their daily living activities ADL and minimal mental state MMSE.

[0149] 3. Experimental results

[0150] The experimental results are as follows Figure 5 As shown in the figure, before treatment, there was no significant difference in ADL scores and MMSE scores between the two groups (P>0.01). After treatment, the ADL scores and MMSE scores of the two groups were significantly improved, especially the scores of the treatment group were significantly better than those of the control group (P>0.05). During the medication period, the treatment group did not experience adverse symptoms such as stomach pain, dizziness, headache, nausea, vomiting, and loss of appetite.

Claims

1. A Chinese medicine composition for promoting blood circulation, unblocking meridians, tonifying the kidney and improving intelligence, comprising red peony root, chuanxiong, rehmannia root, cornus officinalis, white mustard seed, polygala root, calamus calamus, angelica root and poria.

2. The Chinese medicine composition according to claim 1, characterized in that: The mustard is white mustard, and the rehmannia root is cooked rehmannia root.

3. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition comprises the following Chinese medicine components:

4. The Chinese medicine composition according to claim 3, characterized in that: The Chinese medicine composition comprises the following Chinese medicine components:

5. The Chinese medicine composition according to claim 1, wherein the Chinese medicine composition is used in the preparation of a drug for treating and improving memory loss and cognitive dysfunction.

6. The Chinese medicine composition according to claim 5, characterized in that: The medicine is used in preparing medicine for treating dementia.

7. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition can be prepared into conventional clinical oral preparations, specifically granules, mixtures, oral liquids, pills, capsules or tablets.

8. The Chinese medicine composition according to claim 5, characterized in that: The preparation method of the preparation is as follows: the above-mentioned Chinese medicinal materials are crushed and sieved; Poria cocos, Rehmannia glutinosa, Acorus calamus and Polygala tenuifolia are extracted with water to obtain an extract, and the residues are reserved; other remaining medicinal materials and residues are mixed, and a composite enzyme is added for ultrasonic enzymolysis to obtain an enzymatic solution; the extract and the enzymatic solution are mixed, concentrated and dried to obtain an extract, and conventional pharmaceutical excipients are added to prepare a conventional clinical oral preparation.

9. The preparation according to claim 8, characterized in that In the preparation method, the complex enzyme in the enzymatic hydrolysis step includes 9-15 parts by weight of citrate synthase, 4-9 parts by weight of chitinase, and 2-8 parts by weight of mannanase, the temperature of the ultrasonic extraction is 35° C. to 45° C., the power of the ultrasonic extraction is 100W to 180W, and the time is 25min to 45min.

10. The preparation according to claim 8, characterized in that In the preparation method, after adding the complex enzyme in the enzymatic hydrolysis step, the pH value of the mixed solution is adjusted to 5.5-6.5.

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