Solid-phase carrier and matched incubation supporting platform deck
By using magnetic units to fix the solid phase carrier in gene sequencing technology, the problem of insufficient airtightness and incubation performance of the solid phase carrier on the support platform is solved, and higher airtightness and incubation performance is achieved, avoiding the complexity and cost problems of the prior art.
Patent Information
- Application Number
- CN202311180884.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-09-14
- Publication Date
- 2025-03-14
AI Technical Summary
In the existing gene sequencing technology, the fixation method of solid-phase carriers on the support stage has problems such as insufficient airtightness and incubation performance, especially the disadvantages of high complexity, high cost and reduced contact area of negative pressure adsorption and mechanical compression schemes.
The solid phase carrier is fixed by using magnetic units. Through the interaction between the first magnetic unit and the third magnetic unit, the solid phase carrier is closely fitted with the incubation support platform to form a complete fluid path, and improve airtightness and incubation performance.
The tight fit between the solid phase carrier and the support platform is achieved, the air tightness and incubation performance are improved, the disadvantages of negative pressure adsorption and mechanical pressing schemes are avoided, the equipment reliability is improved and the cost is reduced.
Smart Images
Figure CN119614325A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of gene sequencing technology, and in particular to a solid phase carrier and a matching incubation support platform. Background Art
[0002] In the biotechnology industry of gene sequencing, it is usually necessary to transport the samples to be tested and different types of biochemical reagents into the solid phase carrier with the help of external power, and to incubate the fluid in the solid phase carrier at a controlled temperature so that the samples and reagents undergo biochemical reactions. In this process, the solid phase carrier needs to be fixed on the support platform so that the external fluid can be transported to the biological flow pool of the solid phase carrier without leakage, and the heating or cooling of the fluid in the biological flow pool can be completed at a specific time.
[0003] In practical applications, there are generally two solutions to fix the solid phase carrier on the support stage. One is the negative pressure adsorption solution, in which grooves are machined on the contact surface between the support stage and the solid phase carrier, and connected to an external vacuum generating system. A closed channel is formed between these grooves and the surface of the solid phase carrier, and the vacuum generating system is used to make the sealed channel formed by the grooves generate adsorption force, thereby allowing the solid phase carrier to be adsorbed on the support stage. This method requires additional vacuum pumps, air pressure sensors, and gas pipelines, which increases the complexity and cost of the instrument; at the same time, since grooves need to be machined on the surface of the support stage, the contact area between the support stage and the solid phase carrier is reduced, affecting the heating and cooling efficiency.
[0004] The second is a mechanical pressing solution, which is to design mechanical components such as clamping mechanisms and positioning mechanisms on the support platform, and press the solid phase carrier to the specified position of the support platform through the guiding effect of the positioning mechanism and the mechanical force of the clamping mechanism. This method has high requirements for clamping force, positioning accuracy and repeatability, so some parts need to be precisely designed and processed, and a higher support platform assembly process is also required. Moreover, the mechanical force cannot be easily adjusted, and there will be problems such as excessive clamping force causing deformation of the solid phase carrier or excessive clamping force causing poor sealing of the solid phase carrier. Excessive or insufficient clamping force will affect the contact between the solid phase carrier and the support platform, indirectly affecting the airtightness and incubation performance. Summary of the invention
[0005] In order to solve the problems of air tightness and incubation performance in the solid phase carrier flow channel in the gene sequencing biotechnology industry and avoid the disadvantages of negative pressure adsorption and mechanical pressing solutions, it is necessary to provide a solid phase carrier and a matching incubation support platform.
[0006] In order to achieve the above-mentioned purpose, according to one aspect of the present application, a solid phase carrier is provided, including: a biological flow cell, including at least one liquid inlet, at least one liquid outlet and at least one internal flow channel; a shell for fixing the biological flow cell; a first magnetic unit, generating a magnetic force to fix the solid phase carrier at a specific position.
[0007] In some possible implementations, a sealing gasket is further included, which is connected to the liquid inlet and the liquid outlet of the biological flow cell.
[0008] In some possible implementations, the first magnetic unit is located on the housing.
[0009] In some possible implementations, the housing has a first guide hole.
[0010] In some possible implementations, an identification label and a second magnetic unit are further included, and the identification label and the second magnetic unit are located on the housing.
[0011] In some possible implementations, a sealing gasket support is further included, which is used to fix the sealing gasket on the housing so that the sealing gasket is connected to the liquid inlet and the liquid outlet of the biological flow cell.
[0012] According to another aspect of the present application, an incubation support platform is provided, comprising: a first support unit, providing a flat physical support surface with good thermal conductivity; a first incubation unit, connected to the first support unit, to heat or cool it; a first circulation unit, connected to the first incubation unit, to ensure that the contact surface of the two is within a relatively stable temperature range; a first flow unit, comprising at least one fluid channel; and a third magnetic unit, to generate a magnetic force.
[0013] In some possible implementations, the third magnetic unit is located on the first support unit; the magnetic poles generated by the third magnetic unit are opposite to the magnetic poles generated by the first magnetic unit, so that the solid phase carrier can be adsorbed on the incubation support table.
[0014] In some possible implementations, a second supporting unit is further included to provide solid support and connection for the first supporting unit, the first incubation unit, the first circulation unit, and the first flow unit.
[0015] In some possible implementations, a sealing gasket is further included, connected to the fluid channel of the first flow unit.
[0016] In some possible implementations, a first identification unit is further included, and the first identification unit can detect the position of the second magnetic unit.
[0017] In some possible implementations, a first positioning unit is further included, and the first positioning unit is fixed on the first supporting unit.
[0018] In some possible implementations, a first temperature monitoring unit is further included, and the first temperature monitoring unit is used to monitor the temperature state of the first supporting unit.
[0019] Compared with the prior art, in this application, the solid phase carrier and the support platform can be closely fitted to form a complete fluid path through the adjustment of the position of the magnetic unit and the interaction, thereby improving the air tightness and incubation performance. At the same time, there is no need to add additional gas path components or design and assemble high-precision mechanical structures, avoiding the disadvantages of negative pressure adsorption and mechanical pressing solutions, improving equipment reliability and reducing equipment costs. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 : Schematic diagram of the three-dimensional structure of a solid phase carrier and a matching incubation support platform provided in one embodiment of the present application.
[0021] Figure 2 : Schematic diagram of the top view of the solid phase carrier and the matching incubation support platform provided in one embodiment of the present application.
[0022] Figure 3 :for Figure 2 The cross-sectional view of the solid phase carrier and the matching incubation support platform along AA is shown.
[0023] Figure 4 :for Figure 2 The cross-sectional view along BB of the solid phase carrier and the matching incubation support platform is shown.
[0024] Figure 5 :for Figure 2 The cross-sectional view along CC of the solid phase carrier and the matching incubation support platform is shown.
[0025] Figure 6 :for Figure 2 The cross-sectional view of the solid phase carrier and the matching incubation support platform along DD is shown.
[0026] Figure 7 : An exploded view of an incubation support platform provided in one embodiment of the present application.
[0027] Figure 8 : Schematic diagram of the three-dimensional structure of a solid phase carrier provided in one embodiment of the present application.
[0028] Fig. 9 :for Figure 8 Three views of the solid phase carrier shown.
[0029] Fig.10 :for Figure 8 Exploded view of the solid support shown.
[0030] Fig.11 : Schematic diagram of the three-dimensional structure of a solid phase carrier provided in another embodiment of the present application.
[0031] Fig.12 : Schematic diagram of the three-dimensional structure of the incubation support platform provided in another embodiment of the present application.
[0032] Description of main symbols.
[0033] Solid phase carrier 10.
[0034] Housing 101.
[0035] First guide hole 1011.
[0036] Biological flow cell 102.
[0037] Liquid inlet 1021.
[0038] Liquid outlet 1022.
[0039] Runner 1023.
[0040] Sealing gasket 103.
[0041] The first magnetic unit 104 .
[0042] Gasket support 105 .
[0043] The second magnetic unit 106 .
[0044] Identification label 107.
[0045] The support carrier 20 is incubated.
[0046] The first supporting unit 201 .
[0047] The first incubation unit 202 .
[0048] The first cycle unit 203 .
[0049] The first circulation unit 204 .
[0050] Fluid channel 2041.
[0051] The second supporting unit 205 .
[0052] The third magnetic unit 206 .
[0053] The first positioning unit 207 .
[0054] A first temperature monitoring unit 208 .
[0055] The first recognition unit 209 . DETAILED DESCRIPTION
[0056] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present application and its application or use. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.
[0057] It should be noted that the use of terms such as "first" and "second" to limit components is only to facilitate the distinction between corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the present invention.
[0058] It should be noted that the term "and / and / or" used herein includes all and any combination of one or more related listed items. Unless otherwise specifically stated, the relative arrangement of the components and steps, the numerical expressions and the numerical values described in these embodiments do not limit the scope of the present application. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The techniques, methods and equipment known to ordinary technicians in the relevant fields may not be discussed in detail, but in appropriate cases, the techniques, methods and equipment should be regarded as part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, not as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further discussed in subsequent drawings.
[0059] like Figures 1 to 10As shown, the solid phase carrier 10 includes a housing 101, a biological flow pool 102, a sealing gasket 103, a first magnetic unit 104, a sealing gasket support 105, a second magnetic unit 106 and an identification label 107. Among them, the housing 101 includes a first guide hole 1011; the biological flow pool 102 includes a liquid inlet 1021, a liquid outlet 1022 and a flow channel 1023. The biological flow pool 102 is fixed on the housing 101, and the first magnetic unit 104 is fixed on the housing 101 and evenly distributed around the biological flow pool 102. The sealing gasket 103 has a certain elasticity, and the sealing gasket 103 is connected to the housing 101 through the sealing gasket support 105, and is closely connected to the liquid inlet 1021 and the liquid outlet 1022 of the biological flow pool 102. The identification label 107 and the second magnetic unit 106 are located on the housing 101. The identification label 107 stores the production information of the solid phase carrier 10 and can be identified by a scanning device (not shown in this application). The second magnetic unit 106 can emit magnetic lines of force and be detected by the first identification unit 209.
[0060] The incubation support platform 20 includes a first support unit 201, a first incubation unit 202, a first circulation unit 203, a first circulation unit 204, a second support unit 205, a third magnetic unit 206, a first positioning unit 207, a first temperature monitoring unit 208 and a first identification unit 209. Among them, the first circulation unit 204 includes a fluid channel 2041. The first support unit 201 provides a flat physical support surface with good thermal conductivity and contacts the biological flow pool 102 of the solid phase carrier 10. The first incubation unit 202 is located between the first support unit 201 and the first circulation unit 203, and the first support unit 201 is heated or cooled. There is a heat exchange material inside the first circulation unit 203, such as a coolant, which is used to cool or heat the other side of the first incubation unit 202 relative to the first support unit 201, in order to keep the surface temperature relatively stable. The second support unit 205 is a rigid support for the incubation platform 20. The third magnetic unit 206 is evenly fixed on the first support unit 201 along the circumferential direction, and its position corresponds to the first magnetic unit 104. The magnetic poles generated by the third magnetic unit 206 are opposite to those generated by the first magnetic unit 104, so that the solid phase carrier 10 can be adsorbed on the incubation support carrier 20. The first positioning unit 207 is fixed on the first support unit 201, and plays the initial guiding role of placing the solid phase carrier 10 on the incubation support carrier 2. The first identification unit 209 detects the magnetic flux lines of the second magnetic unit 106 to detect whether the solid phase carrier 10 is placed on the incubation support carrier 20. The first temperature monitoring unit 208 is a temperature sensor, which monitors the temperature state of the first support unit 201 when the first incubation unit 202 is working.
[0061] In a conventional workflow, the solid phase carrier 10 needs to be placed on the incubation support stage 20 for sample or reagent loading and subsequent incubation temperature control.
[0062] First, the solid phase carrier 10 is placed on the incubation support platform 20 along the first guide hole 1011 on the housing 101, aligned with the first positioning unit 207. Because the first guide hole 1011 has a guide fillet, the solid phase carrier 10 can be placed on the incubation support platform 20 very conveniently. When the solid phase carrier 10 moves downward due to gravity and gradually approaches the first support unit 201 of the incubation support platform 20, the first magnetic unit 104 on the housing 101 and the third magnetic unit 206 on the first support unit 201 of the incubation support platform 20 cause the solid phase carrier 10 to continue to descend under the action of the magnetic force of mutual attraction, and finally, under the action of the magnetic force, the sealing pad 103 of the solid phase carrier 10 is tightly fitted with the first flow unit 204 of the incubation support platform 20. Since the sealing gasket 103 of the solid phase carrier 10 is connected to the biological flow pool 102, a closed channel is formed by the fluid channel 2041, the sealing gasket 103, the liquid inlet 1021, the flow channel 1023 and the liquid outlet 1022. Moreover, at this time, the biological flow pool 102 is tightly fitted with the first support unit 201, and the second magnetic unit 106 is close to the first identification unit 209, so that the control system (not shown in this application) knows that the process of placing the solid phase carrier 10 is in a normal state.
[0063] When it is necessary to heat or cool the fluid in the flow channel 1023 of the biological flow pool 102 in the solid phase carrier 10, the first incubation unit 202 and the first circulation unit 203 are operated. When the first temperature monitoring unit 208 detects that the first support unit 201 reaches the target temperature, the first incubation unit 202 and the first circulation unit 203 stop working. This process is repeated until the incubation cycle ends.
[0064] like Fig.11 As shown in FIG. 12 , in other embodiments, the first guide hole 1011 on the housing 101 and the first positioning unit 207 corresponding to the incubation support carrier 20 can be eliminated, and the solid phase carrier 10 and the incubation support carrier 20 can be quickly fitted together by only relying on the closed magnetic flux lines generated by the first magnetic unit 104 of the solid phase carrier 10 and the third magnetic unit 206 of the incubation support carrier 20. By controlling the relative position of the first magnetic unit 104 and the third magnetic unit 206 in the horizontal direction and the relative distance in the vertical direction, it can be ensured that the channel formed by the fluid channel 2041, the sealing gasket 103, the liquid inlet 1021, the flow channel 1023 and the liquid outlet 1022 is airtight.
[0065] like Fig.11As shown in FIG12 , in other embodiments, the sealing gasket 103 may be placed on the incubation support carrier 20 , and the solid phase carrier 10 is not provided with the sealing gasket 103 .
[0066] It should be noted that the above embodiments are only used to illustrate the technical solution of the present application and are not intended to limit it. Although the present application has been described in detail with reference to the preferred embodiments, a person skilled in the art should understand that the technical solution of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present application.
Claims
1. A solid phase carrier, characterized in that include: A biological flow cell comprising at least one liquid inlet, at least one liquid outlet and at least one internal flow channel; A housing, the housing being used to fix the biological flow cell; The first magnetic unit generates a magnetic force to fix the solid phase carrier at a specific position.
2. The solid phase carrier according to claim 1, characterized in that It also includes a sealing pad connected to the liquid inlet and the liquid outlet of the biological flow pool.
3. The solid phase carrier according to claim 1, characterized in that The first magnetic unit is located on the housing.
4. The solid phase carrier according to claim 1, characterized in that The housing is deposited in the first guide hole.
5. The solid phase carrier according to claim 1, characterized in that It also includes an identification label and a second magnetic unit, and the identification label and the second magnetic unit are located on the shell.
6. The solid phase carrier according to claims 1 and 2, characterized in that It also includes a sealing pad support member for fixing the sealing pad on the housing so that the sealing pad is connected to the liquid inlet and the liquid outlet of the biological flow cell.
7. An incubation support platform, characterized in that: include: The first support unit provides a flat physical support surface with good thermal conductivity; A first incubation unit, connected to the first supporting unit, for heating or cooling the first supporting unit; A first circulation unit, connected to the first incubation unit, to ensure that the contact surface of the two is within a relatively stable temperature range; A first flow unit includes at least one fluid channel; The third magnetic unit generates a magnetic force.
8. The solid phase carrier and the incubation support platform according to claims 1 and 7, characterized in that: The third magnetic unit is located on the first supporting unit; the magnetic poles generated by the third magnetic unit are opposite to the magnetic poles generated by the first magnetic unit, so that the solid phase carrier can be adsorbed on the incubation supporting table.
9. The incubation support platform according to claim 7, characterized in that: Also included is a second supporting unit, which provides solid support and connection for the first supporting unit, the first incubation unit, the first circulation unit and the first flow unit.
10. The incubation support platform according to claim 7, characterized in that: It also includes a sealing gasket connected to the fluid channel of the first flow unit.
11. The solid phase carrier and the incubation support platform according to claims 5 and 7, characterized in that: The device further includes a first identification unit, which can detect the position of the second magnetic unit.
12. The incubation support platform according to claim 7, characterized in that: It also includes a first positioning unit, which is fixed on the first supporting unit.
13. The incubation support platform according to claim 7, characterized in that: The device further comprises a first temperature monitoring unit, wherein the first temperature monitoring unit is used to monitor the temperature state of the first supporting unit.