Sambucus williamsii hance and sambucus williamsii hance cyanine extraction drink as well as preparation

Abstract

The invention discloses a sambucus williamsii hance and sambucus williamsii hance cyanine extraction beverage as well as a preparation method and application thereof, and belongs to the technical field of foods. Comprising the following steps: mixing and dissolving, filtering, filling and sterilizing. By using the d-ribose, the sambucus williamsii concentrated juice, the sambucus williamsii flower extract, the yeast beta-glucan, the vitamin C, the vitamin B-rich vitamin yeast powder and other raw materials, the health-care food can resist viruses, activate an immune system, improve immunity and prevent and relieve influenza and other symptoms, and nine molecules jointly consolidate and maintain the immune system.

Description

Technical Field

[0001] The invention belongs to the technical field of food, and particularly relates to an elderberry and elderflower extract drink and a preparation method and application thereof. Background Art

[0002] According to a global survey conducted by WHO, only 5% of the population is healthy, 20% is sick, and 75% is in a sub-healthy state. In my country, about 15% of the population is healthy, 15% is unhealthy, and 70% (about 900 million) is in a sub-healthy state. It can be seen that most people are in a sub-healthy state, and sub-health is caused by low immunity.

[0003] Immunity refers to the ability of the human immune system to resist diseases, infections and harmful microorganisms. The ingredients of the products currently available to improve immunity are complex, and the taste is not good and the effective amount is insufficient. Therefore, it is necessary to develop a product that improves immunity, with simple ingredients, good taste, sufficient effective amount, and few products with anti-fatigue and immunity-enhancing effects. The present invention uses d-ribose, elderberry concentrated juice, yeast beta-glucan, etc. as main raw materials, and the oral liquid prepared with various fruit juices is sweet and sour and refreshing in taste, wherein elderberry concentrated juice and elderflower extract can effectively resist viruses, yeast beta-glucan can activate immune cells, vitamin C can promote the production of antibodies, improve the ability to resist viruses, d-ribose can maintain the energy supply of the entire immune system, and is matched with B vitamins yeast powder, which jointly maintain and improve the function of the immune system, prevent and relieve symptoms such as colds and flu, and relieve fatigue. Summary of the invention

[0004] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0005] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0006] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide an elderberry and elderflower extract drink.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions, including:

[0008] Water, elderberry juice concentrate, elderflower extract, vitamins, d-ribose and / or yeast beta-glucan;

[0009] It also includes yeast powder and fruit juice.

[0010] As a preferred embodiment of the elderberry and elderflower extract drink of the present invention, the following components are included in terms of weight percentage of each raw material:

[0011] 26.4-99 parts of water, 0.1-12 parts of elderberry concentrated juice, 0.1-0.68 parts of elderflower extract, 0.1-3 parts of vitamins, 0-20 parts of d-ribose, 0-1.6 parts of yeast β-glucan, 0-1.8 parts of yeast powder, and 0-34.5 parts of fruit juice.

[0012] As a preferred embodiment of the elderberry and elderflower extract drink of the present invention, the following components are included in terms of weight percentage of each raw material:

[0013] 40-90 parts of water, 3.3-9 parts of elderberry concentrated juice, 0-0.4 parts of elderflower extract, 0.1-1 parts of vitamins, 5-15 parts of d-ribose, 0-1.6 parts of yeast β-glucan, 0-1 part of yeast powder, and 0-11.5 parts of fruit juice.

[0014] As a preferred embodiment of the elderberry and elderflower extract drink of the present invention, the following components are included in terms of weight percentage of each raw material:

[0015] 50-80 parts of water, 3.3-6.7 parts of elderberry concentrated juice, 0-0.34 parts of elderflower extract, 0.1-1 parts of vitamins, 5-10 parts of d-ribose, 0-1 parts of yeast β-glucan, 0-0.5 parts of yeast powder, and 0-11.5 parts of fruit juice.

[0016] As a preferred embodiment of the elderberry and elderflower extract drink of the present invention, the added amount of the elderberry concentrated juice is 6.7 parts.

[0017] As a preferred embodiment of the elderberry and elderflower extract drink of the present invention, the added amount of the elderflower extract is 0.3 parts.

[0018] As a preferred embodiment of the elderberry and elderflower extract drink described in the present invention, the vitamins include vitamin C and vitamin B.

[0019] As a preferred embodiment of the elderberry and elderflower extract drink described in the present invention, the juice includes one or more of red grape concentrated juice, blueberry concentrated juice, and red raspberry concentrated juice.

[0020] As a preferred embodiment of the elderberry and elderflower extract drink described in the present invention, the mass ratio of the red grape concentrated juice, the blueberry concentrated juice and the red raspberry concentrated juice is 0-15:0-3:0-5.

[0021] Another object of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing an elderberry and elderflower extract drink.

[0022] In order to solve the above technical problems, the present invention provides the following technical solutions, including:

[0023] Elderberry juice concentrate, elderflower extract, vitamins, d-ribose, yeast beta-glucan, yeast powder, juice, add a small amount of water, stir to dissolve, mix evenly;

[0024] Add water to make up the mixed solution to volume, mix well, filter and fill;

[0025] After heating and sterilization, elderberry and elderflower extract drink is obtained.

[0026] The third purpose of the present invention is to overcome the deficiencies in the prior art and provide an application of elderberry and elderflower extract drink in improving the body's immunity.

[0027] Beneficial effects of the present invention:

[0028] The elderberry and elderflower extract drink prepared by the present invention has a clean and concise ingredient list, contains D-ribose, elderberry concentrated juice, yeast β-glucan, elderflower extract, vitamins, yeast powder and other effective ingredients, and is seasoned with fruit juice. It is sweet and sour compared with products on the market, has a refreshing taste, and has a simple formula. It can effectively prevent and relieve symptoms such as colds and influenza, and improve immunity. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0030] Figure 1 This is a photo of the liquid inside the elderberry and elderflower extract drink;

[0031] Figure 2 This is the actual product picture of elderberry and elderflower extract drink. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0033] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0035] The raw materials used in the present invention are all commercially available unless otherwise specified.

[0036] The red grape concentrate used in the embodiments of the present invention is supplied by VINOS & BODEGAS of Spain (sugar content: 65±2 Brix, color: 500-700); elderberry concentrate, red raspberry concentrate and blueberry concentrate are provided by Wither Amer Foods of the United States (product codes are 14876.2, 4237.9 and 5182.4 respectively); elderflower extract is provided by Iprona of Italy (product code is 60234501).

[0037] Example 1

[0038] This embodiment provides a method for preparing elderberry and elderflower extract drink, specifically:

[0039] Take 6.7 parts of elderberry concentrated juice, 0.34 parts of elderflower extract, 0.7 parts of vitamin C, 10 parts of d-ribose, 0.8 parts of yeast β-glucan, 0.2 parts of yeast powder, 7.5 parts of red grape concentrated juice, 1.5 parts of blueberry concentrated juice, and 2.5 parts of red raspberry concentrated juice (i.e., the mass ratio of red grape concentrated juice, blueberry concentrated juice, and red raspberry concentrated juice is 15:3:5), add 30 parts of water, stir to dissolve, and mix evenly;

[0040] The mixed solution is diluted to 100 parts with water, mixed evenly, filtered and filled;

[0041] After heating and sterilizing at 90° C. for 30 minutes, the elderberry and elderflower extract drink was obtained.

[0042] Example 2

[0043] The difference between this embodiment and embodiment 1 is that the addition amount of elderberry concentrated juice is adjusted to 3.3 parts, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderberry flower essence drink.

[0044] Example 3

[0045] The difference between this embodiment and embodiment 1 is that the addition amount of elderberry concentrated juice is adjusted to 9 parts, and the rest of the preparation process is the same as that of embodiment 1, to prepare elderberry and elderflower essence drink.

[0046] Example 4

[0047] The difference between this embodiment and embodiment 1 is that the addition amount of elderberry concentrated juice is adjusted to 12 parts, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderberry flower essence drink.

[0048] Comparative Example 1

[0049] The difference between this comparative example and Example 1 is that the addition amount of elderberry concentrated juice is adjusted to 0, and the rest of the preparation process is the same as that of Example 1 to prepare elderberry and elderberry flower essence drink.

[0050] Example 5

[0051] The difference between this embodiment and embodiment 1 is that the addition amount of elderberry flower extract is adjusted to 0.68 parts, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderberry flower essence drink.

[0052] Comparative Example 2

[0053] The difference between this comparative example and Example 1 is that the addition amount of the elderberry flower extract is adjusted to 0, and the rest of the preparation process is the same as that of Example 1 to prepare elderberry and elderberry flower essence drink.

[0054] Example 6

[0055] The difference between this embodiment and embodiment 1 is that the amount of d-ribose added is adjusted to 0, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderberry flower essence drink.

[0056] Example 7

[0057] The difference between this embodiment and embodiment 1 is that the amount of d-ribose added is adjusted to 5 parts, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderflower extract drink.

[0058] Example 8

[0059] The difference between this embodiment and embodiment 1 is that the amount of d-ribose added is adjusted to 15 parts, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderflower extract drink.

[0060] Example 9

[0061] The difference between this embodiment and embodiment 1 is that the amount of d-ribose added is adjusted to 20 parts, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderflower extract drink.

[0062] Example 10

[0063] The difference between this embodiment and embodiment 1 is that the amount of red grape concentrated juice added is adjusted to 11.5 parts, the amount of blueberry concentrated juice and red raspberry concentrated juice added is adjusted to 0, and the rest of the preparation process is the same as that of embodiment 1, and elderberry and elderflower essence drink is prepared.

[0064] Embodiment 11

[0065] The difference between this embodiment and embodiment 1 is that the amount of blueberry concentrated juice added is adjusted to 11.5 parts, the amount of red grape concentrated juice and red raspberry concentrated juice added is adjusted to 0, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderberry flower essence drink.

[0066] Example 12

[0067] The difference between this embodiment and embodiment 1 is that the amount of red raspberry concentrated juice added is adjusted to 11.5 parts, the amount of blueberry concentrated juice and red grape concentrated juice added is adjusted to 0, and the rest of the preparation process is the same as that of embodiment 1 to prepare elderberry and elderflower essence drink.

[0068] Sensory evaluation:

[0069] 30 sensory evaluators were recruited to conduct sensory evaluation on the elderberry and elderflower extract drinks prepared in the above examples and comparative examples, and the evaluation criteria are shown in Table 1. The dosage for each serving was 15 mL, and the evaluation results are shown in Table 2.

[0070] Table 1 Comprehensive score table for sensory evaluation

[0071]

[0072] Table 2 Sensory evaluation of elderberry and elderflower extract drink

[0073]

[0074]

[0075] As can be seen from Table 2, the change in the amount of D-ribose added has a greater sensory impact on the elderberry and elderflower extract drink. When the added amount increases, the sweetness increases, and the sweet and sour ratio is appropriate. When the added amount exceeds 10 parts, the overall taste is sweet, and the taste of the D-ribose raw material itself begins to gradually appear, covering up the fruity aroma and weakening the fruity aroma. The preferred addition amount is 10 parts.

[0076] The amount of elderflower extract added mainly affects the residual feeling after consumption of the elderberry and elderflower extract drink. When the amount of elderflower extract added is 0-0.34 parts, it has almost no effect on the sensory organs. When the amount added is increased to 0.68 parts, a slight residual spicy feeling begins to appear.

[0077] Elderberry concentrate juice is rich in polyphenols such as anthocyanins and total flavonoids, so it tastes bitter. When the amount of elderberry concentrate added is within a certain range, its bitterness can be masked by the sourness and sweetness. When the elderberry concentrate is added to more than 6.7 parts, the bitterness of the raw material gradually emerges.

[0078] In addition to elderberry concentrate juice, the product also contains three kinds of fruit juices. The effects of using other single fruit juices and the combination of the three fruit juices on the sensory organs were investigated. If only red grape concentrate juice was used, the taste would be slightly sweeter overall, while if only blueberry concentrate juice or red raspberry concentrate juice was used, the taste would be sour. If only one of the three juices was used, the overall taste would not be soft or suitable enough. Therefore, the combination of the three fruit juices gave the best overall taste.

[0079] Stability test

[0080] The unfiltered elderberry and elderflower extract drink of Example 1 (sample 1) and the elderberry and elderflower extract drink of Example 1 (sample 2) were placed in a 15 mL roll film bag, and an accelerated stability test was conducted. The bags were stored at a temperature of 37±2°C, a relative humidity of RH 75±5%, and were protected from direct sunlight for 3 months. The samples that were stored for 0, 1, 2, and 3 months were examined, and the sensory organs, total bacterial count, and the content of anthocyanins and total flavonoids in the samples were tested. The sensory organs were tested using the method of Experimental Example 1, the total bacterial count was determined using the "GB 4789.2-2022 National Food Safety Standard for Food Microbiological Examination of Total Bacterial Counts", the total flavonoids were determined using Appendix G of the "GB / T 12143-2008 General Analysis Method for Beverages", and the total flavonoids were determined using the "GB / T 12143-2008 General Analysis Method for Beverages".

[0081] The anthocyanin content was determined according to NY / T 2640-2014 Determination of Anthocyanins in Plant-derived Foods by High Performance Liquid Chromatography, and the quality of elderberry and elderflower extract beverage was compared before and after accelerated storage. The results are shown in Tables 3 to 6 below:

[0082] Table 3 Sensory changes of sample 1 during the accelerated test

[0083]

[0084] As can be seen from the table, after 2 months of accelerated experiment, the unfiltered sample 1 showed slight precipitation, and a slight off-flavor could be tasted. After 3 months of accelerated experiment, the color became darker, and it felt slightly turbid, with more precipitation and a slight off-flavor, indicating that the filtration operation had a greater impact on the overall state of the sample, and omitting the filtration step would cause precipitation in the sample.

[0085] Table 4 Sensory changes of sample 2 during the accelerated test

[0086]

[0087]

[0088] As can be seen from the table, during the accelerated experiment, there was no obvious change in Sample 2 as a whole, indicating that the product stability has been maintained very well, which may be closely related to the filtration in the production process.

[0089] Table 5 Changes in total colony count, anthocyanins and total flavonoids of sample 1 during the accelerated experiment

[0090]

[0091] The results in Table 5 show that the total colony count during the accelerated experiment was always <1, indicating that the non-filtration operation did not affect the microbial indicators of the product and did not cause significant growth of microorganisms during the accelerated experiment; however, the attenuation rates of anthocyanin and total flavonoids after 3 months of accelerated experiment were 6.69% and 8.05%, respectively, which were higher than the attenuation rates after the filtration operation, indicating that the filtration operation would affect stability, and reduced stability would also cause the functional ingredients to decay faster.

[0092] Table 6 Changes of total colony count, anthocyanins and total flavonoids in sample 2 during the accelerated experiment

[0093]

[0094] The results in Table 6 show that the total colony count during the accelerated experiment was always <1, indicating that the microbial indicators remained stable during the accelerated experiment; and the decay rates of anthocyanins and total flavonoids during the 3-month accelerated experiment were 1.96% and 1.80%, indicating that higher temperatures (37°C) and the extension of time will cause the loss of functional ingredients, but lower than the samples without filtration, indicating that the filtration operation helps maintain the stability of the beverage system. During the entire accelerated experiment, the total colony count, anthocyanins and total flavonoids were within the quality error range, indicating that the formula and process are feasible and can maintain the quality of the sample within the shelf life.

[0095] Mouse experiments

[0096] 1. Experimental Setup

[0097] In this experiment, SPF male mice weighing 18-22 g were selected. After one week of adaptive feeding, they were randomly divided into 5 groups: normal control group, low-dose group, medium-dose group, and high-dose group, with 15 mice in each group. The mice were gavaged continuously for 30 days.

[0098] The recommended daily intake of elderberry and elderflower extract is 15 mL. According to the average weight of Chinese adult men and women of 60 kg, the oral dose is set as low, medium and high dose groups, which are 5 times, 10 times and 30 times the daily intake of adults, respectively. The values ​​converted to mice are 1.25, 2.5 and 3.75 mL / kg bw·d, that is, about 0.25, 0.5 and 0.75 mL of oral dose each time, once a day. The mice in the control group were given oral administration with an equal amount of distilled water.

[0099] 2. Measurement indicators

[0100] After the experiment, referring to the method of "Health Food Function Test and Evaluation Method (2023 Edition)", the weight, delayed-type hypersensitivity (DTH), antibody-producing cells (PFC), mouse carbon clearance test, weighted swimming time, serum urea, liver glycogen content, and blood lactate value of mice were measured.

[0101] The experiment referred to the "Health Food Function Test and Evaluation Methods (2023 Edition)", and sheep red blood cells (SRBC) were used to induce mouse DTH (plantar thickening method) to measure delayed-type hypersensitivity (DTH), Jerne's modified slide method to measure antibody-producing cells (PFC), an automatic biochemical analyzer to measure serum urea, and the anthrone method to measure liver glycogen content.

[0102] 3. The results are shown in Tables 7 to 14 below:

[0103] Table 7 Effects of elderberry and elderflower extract on body weight of mice

[0104] Group Number of animals Initial body weight (g) Final weight (g) Normal control group 15 20.1±1.3 35.7±2.5 Low dose group 15 20.3±0.9# 34.8±1.9# Medium dose group 15 19.8±1.1# 35.5±2.3# High dose group 15 19.9±1.3# 35.3±3.4#

[0105] (Note: #P>0.05 indicates that there is a significant difference between the experimental group and the control group)

[0106] As shown in Table 7, compared with the normal control group, the initial weight of mice in the three different dose groups had no significant difference (P>0.05), and the selected mice met the requirements of the experiment. After 30 days of gavage, there was still no significant difference between the three experimental groups and the normal control group (P>0.05), indicating that the elderberry elderflower extract drink did not affect the weight gain of mice.

[0107] Table 8 Effects of elderberry and elderflower extract on delayed-type hypersensitivity (DTH) in mice

[0108] Group Number of animals Plantar swelling (mm) Normal control group 15 0.71±0.15 Low dose group 15 0.85±0.1 Medium dose group 15 0.95±0.13 High dose group 15 0.99±0.1

[0109] SRBC can stimulate T lymphocytes to proliferate into sensitized lymphocytes. After 4 days, when attacked with SRBC again, swelling occurs at the attack site, and the degree of swelling can reflect the degree of delayed-type hypersensitivity. Table 8 reflects the effect of elderberry and elderflower extract drink on delayed-type hypersensitivity (DTH) in mice. Compared with the normal control group, the difference in toe thickness (24h and 0h) of mice in the low, medium and high dose experimental groups after being attacked by SRBC was significantly increased, indicating that elderberry and elderflower extract drink can enhance delayed-type hypersensitivity in mice. Intake of elderberry and elderflower extract drink can promote the body's cellular immune response, which may be related to the high content of elderberry concentrated juice, yeast β-glucan, vitamin C, yeast powder rich in B vitamins, etc. in the sample. Studies have shown that elderberry juice and yeast β-glucan can activate immune cells and enhance immune response.

[0110] Table 9 Effects of elderberry elderflower extract on mouse antibody-producing cells (PFC)

[0111] Group Number of animals <![CDATA[Hemolytic plaque number / 10 5 splenocytes]]> Normal control group 15 68.9±9.9 Low dose group 15 92.8±11.1 Medium dose group 15 99.9±13.3 High dose group 15 105.9±14.2

[0112] PFC determination technology is also called hemolytic plaque test, which is one of the methods for detecting single antibody-forming cells in vitro. As shown in Table 9, the low, medium and high dose groups can increase the number of hemolytic plaques in mice, and there is a positive correlation with the dose, indicating that the samples can promote the production of antibodies and enhance humoral immunity. This is related to the elderflower extract and various juices in the samples as well as the additional vitamin C, which are essential for the normal function of the immune system and help to increase the production of antibodies.

[0113] Table 10 Effects of elderberry and elderflower extract on carbon clearance experiment in mice

[0114] Group Number of animals Phagocytic index a Normal control group 15 4.51±0.59 Low dose group 15 5.67±0.67 Medium dose group 15 6.36±0.91 High dose group 15 6.92±1.03

[0115] Monocytes and macrophages perform immune functions by phagocytosis, so their phagocytic ability is one of the main ways to reflect immune function. The mouse carbon clearance experiment was used to determine the function of monocytes and macrophages, and the phagocytic index was used to represent the carbon clearance ability of mice. As shown in Table 10, elderberry and elderflower extract drink can significantly improve the phagocytic index and effectively promote the phagocytic ability of monocytes and macrophages. This function may be related to yeast β-glucan, which can activate immune cells, including monocytes and macrophages, thereby enhancing their phagocytic ability.

[0116] Table 11 Effects of elderberry and elderflower extract on weight-bearing swimming time in mice

[0117] Group Number of animals Weighted swimming time (min) Normal control group 15 11.3±1.5 Low dose group 15 12.4±1.8 Medium dose group 15 13.7±1.5 High dose group 15 14.3±2.1

[0118] The weighted swimming time of mice is the most direct indicator of the anti-fatigue ability of elderberry extract. Compared with the normal control group, the weighted swimming time of the experimental groups with different doses was prolonged, indicating that the samples can improve exercise endurance to a certain extent and have a certain anti-fatigue effect. This may be related to the presence of elderberry concentrate and d-ribose in the samples. Studies have shown that both have anti-fatigue effects.

[0119] Table 12 Effects of elderberry and elderflower extract on serum urea in mice

[0120] Group Number of animals Serum urea (mmol / L) Normal control group 15 12.1±1.5 Low dose group 15 10.9±1.6 Medium dose group 15 9.4±1.1 High dose group 15 8.9±1.2

[0121] During strenuous exercise, the body needs a lot of energy to maintain exercise, but blood sugar supply may be insufficient at this time. In order to make up for the energy gap, the body accelerates the decomposition of proteins and amino acids, resulting in an increase in the concentration of urea nitrogen in the body. The increase in urea nitrogen content can be regarded as an indicator of the body's fatigue level during exercise. The serum urea content of the experimental group was significantly lower than that of the normal control group, indicating that the sample has an important relationship with reducing the decomposition and utilization of proteins during exercise.

[0122] Table 13 Effect of elderberry and elderflower extract on liver glycogen content in mice

[0123] Group Number of animals Liver glycogen content / (mg / 100g liver) Normal control group 15 3852±545 Low dose group 15 4213±523 Medium dose group 15 4491±621 High dose group 15 4547±612

[0124] Studies have found that the body's energy consumption after exercise is synchronized with the consumption of stored glycogen (muscle glycogen). In order to maintain the blood sugar level required for exercise, the body will continue to use glycogen in the liver. If the body's glycogen reserves are sufficient, the onset of fatigue can be delayed. Therefore, the content of glycogen is considered to be an important indicator for assessing the body's fatigue level. Compared with the normal control group, the glycogen content of the medium and high-dose groups increased significantly, especially the high-dose group, indicating that the elderberry essence drink may play a role by increasing the body's glycogen reserves or reducing its consumption during exercise, which is closely related to the d-ribose and elderberry concentrate juice.

[0125] Table 14 Effects of elderberry and elderflower extract on the area under the blood lactate curve of mice at three time points

[0126] Group Number of animals Area under the blood lactate curve at three time points (mmol / L) Normal control group 15 121.7±17.5 Low dose group 15 105.3±14.6 Medium dose group 15 94.1±12.1 High dose group 15 89.4±13.2

[0127] When exercising, lactic acid is a metabolite produced in muscles. If lactic acid accumulates too much in the body, it will lead to a decrease in exercise capacity. Therefore, the lactic acid content in the blood is often used as a key indicator to determine the degree of fatigue. After taking different doses of elderberry extract for 30 days, the area under the blood lactate curve decreased, indicating that the sample may help increase the rate of energy synthesis, thereby slowing down the accumulation of lactic acid in the blood.

[0128] In short, according to the results of the mouse experiment, elderberry and elderflower extract drink can improve the body's immunity by enhancing cellular immunity, humoral immunity, and the phagocytic ability of mononuclear-macrophages, not from one aspect, but from all aspects to improve the overall immunity. This is related to the elderberry concentrate juice, yeast β-glucan, elderflower extract, vitamin C, and vitamins rich in B group in the sample; in addition, the sample can help relieve physical fatigue to a certain extent by extending the time of mice swimming with weights, reducing the urea content in the blood, increasing the content of liver glycogen, and reducing the accumulation of lactic acid. The main effect is the elderberry concentrate juice and d-ribose. Taken together, these two aspects may play an important role in preventing and relieving symptoms such as colds and flu.

[0129] Clinical application experiment

[0130] 1. Subjects

[0131] A total of 135 volunteers (age range 16 to 60 years) with influenza symptoms for less than 48 hours between June 2023 and December 2023 were screened. The inclusion criteria were that all volunteers had at least three other respiratory influenza symptoms (fever ≥38°C, headache, muscle aches, cough, runny nose, and nasal congestion). Exclusion criteria included pregnancy or breastfeeding; suspected mixed infection with other pathogens such as bacteria and mycoplasma; recent antiviral treatment; recent participation in another clinical trial; allergy to ingredients; vaccination against influenza and treatment for chronic diseases; severe or critical cases such as altered consciousness, pneumonia, respiratory failure, etc.; and severe underlying diseases such as liver, kidney, immune function, cardiovascular, neurological, or cardiopulmonary diseases. Except for this influenza outbreak, the subjects were all healthy individuals and did not belong to the high-risk group. Written informed consent was obtained from each patient before enrollment.

[0132] 2. Experimental settings and measurement indicators

[0133] The subjects were randomly divided into a control group, an experimental group 1 and an experimental group 2. Each person in the experimental group took 2 bags of 15 mL elderberry extract drink and the elderberry and elderflower extract drink without d-ribose described in Example 2, once a day. The control group took a commercially available drink that was basically the same as the experimental group in packaging, appearance, color, and taste for 5 days.

[0134] Clinical symptoms were scored as 0, 1, 2, and 3 points for none, mild, moderate, and severe, respectively. The time for symptom disappearance during medication was recorded, i.e., the time for complete fever reduction (the time for the patient's axillary temperature to drop below 37.5°C), myalgia, headache, general malaise, cough, runny nose, and nasal congestion. These symptoms were assessed at the beginning of treatment (baseline) to investigate whether the three groups were clinically comparable at the beginning of treatment.

[0135] 3. The results are shown in Tables 15 to 17 below:

[0136] Table 15 Comparison of influenza symptom scores in the three groups before taking

[0137] Flu symptoms Control group Experimental group 1 (D-ribose group) Experimental group 2 (no d-ribose group) fever 2.31±2.01 2.16±1.69# 2.35±1.32# Headache 2.38±1.23 2.45±2.01# 2.42±1.89# Myalgia 1.76±0.72 1.83±1.03# 1.79±1.13# cough 2.34±1.93 2.42±2.11# 2.29±1.77# Runny nose 1.85±0.98 1.75±1.32# 1.79±1.12# Nasal congestion 2.24±1.78 2.32±2.16# 2.28±2.19#

[0138] (Note: #P>0.05 indicates that there is no significant difference between the experimental group and the control group)

[0139] Table 15 shows the influenza symptom scores of the three groups of volunteers before taking the drug. There is no significant difference among the three groups, indicating that they are comparable.

[0140] Table 16 Comparison of influenza symptom scores among three groups after taking for 48 hours

[0141] Flu symptoms Control group Experimental group 1 (D-ribose group) Experimental group 1 (no d-ribose group) fever 1.38±0.78 0.35±0.48 0.89±0.78 Headache 1.48±1.12 0.45±0.67 0.95±0.56 Myalgia 1.35±0.87 0.17±0.42 0.85±0.89 cough 1.96±1.34 1.09±0.86 1.56±1.32 Runny nose 1.47±0.79 0.95±1.02 1.25±1.18 Nasal congestion 1.56±1.12 0.57±0.63 1.11±0.85

[0142] Table 16 shows the flu symptom scores of the three groups of volunteers 48 hours after taking the medicine. Among the three groups, the d-ribose version of elderberry and elderflower extract drink has the best effect in relieving colds. Compared with taking other similar products on the market, both the versions with and without d-ribose can significantly improve the symptoms of influenza, especially relieve myalgia. The addition of d-ribose may provide additional energy by increasing the synthesis of ATP, which helps cell function and muscle recovery. Compared with the elderberry and elderflower extract drink without d-ribose, the addition of d-ribose is more conducive to overall recovery. During the flu period, not only elderberry concentrated juice and elderflower extract are needed to resist viruses and inflammation, but also yeast β-glucan is needed to activate immune cells and enhance the immune system's response ability, as well as vitamin C to produce antibodies and enhance the body's ability to resist viruses, while d-ribose is used to quickly supply energy to maintain the normal operation of the entire system, and yeast powder rich in B vitamins assists in maintenance. These ingredients work together to maintain and improve the function of the immune system.

[0143] Table 17 Comparison of symptom disappearance time among three groups

[0144]

[0145]

[0146] Table 17 is a comparison of the disappearance time of the three groups of symptoms. It is consistent with the trend of Table 14. Compared with taking the same and similar products on the market, the d-ribose version of elderberry and elderflower extract drink can completely eliminate cold symptoms more than one day earlier, and the non-d-ribose group can recover half a day earlier. This is closely related to the combination of functional ingredients in the product.

[0147] In summary, the elderberry and elderflower extract drink prepared by the present invention has a clean and concise ingredient list, contains D-ribose, elderberry concentrated juice, yeast β-glucan, elderflower extract, vitamins, yeast powder and other effective ingredients, supplemented with fruit juice for flavoring, is sweet and sour compared to products on the market, has a refreshing taste, a simple formula, and can effectively prevent and relieve symptoms such as colds and flu, and improve immunity.

[0148] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. An elderberry and elderflower extract drink, characterized in that: Calculated by weight of each raw material, including: 26.4-99 parts of water, 0.1-12 parts of elderberry concentrated juice, 0.1-0.68 parts of elderflower extract, 0.1-3 parts of vitamins, 0-20 parts of d-ribose, 0-1.6 parts of yeast β-glucan, 0-1.8 parts of yeast powder, and 0-34.5 parts of fruit juice.

2. The elderberry and elderflower extract drink according to claim 1, characterized in that: Calculated by weight of each raw material, including: 40-90 parts of water, 3.3-9 parts of elderberry concentrated juice, 0-0.4 parts of elderflower extract, 0.1-1 parts of vitamins, 5-15 parts of d-ribose, 0-1.6 parts of yeast β-glucan, 0-1 part of yeast powder, and 0-11.5 parts of fruit juice.

3. The elderberry and elderflower extract drink according to claim 1, characterized in that: Calculated by weight of each raw material, including: 50-80 parts of water, 3.3-6.7 parts of elderberry concentrated juice, 0-0.34 parts of elderflower extract, 0.1-1 parts of vitamins, 5-10 parts of d-ribose, 0-1 parts of yeast β-glucan, 0-0.5 parts of yeast powder, and 0-11.5 parts of fruit juice.

4. The elderberry and elderflower extract drink according to any one of claims 1 to 3, characterized in that: The added amount of the elderberry concentrated juice is 6.7 parts.

5. The elderberry and elderflower extract drink according to any one of claims 1 to 3, characterized in that: The added amount of the elderflower extract is 0.34 parts.

6. The elderberry and elderflower extract drink according to any one of claims 1 to 3, characterized in that: The vitamins include vitamin C and vitamin B.

7. The elderberry and elderflower extract drink according to any one of claims 1 to 3, characterized in that: The fruit juice comprises one or more of red grape concentrated juice, blueberry concentrated juice and red raspberry concentrated juice.

8. The elderberry and elderflower extract drink according to claim 7, characterized in that: The mass ratio of the red grape concentrated juice, the blueberry concentrated juice and the red raspberry concentrated juice is 0-15:0-3:0-5.

9. The method for preparing the elderberry and elderflower extract drink according to claim 1, characterized in that: include, Take elderberry concentrated juice, elderflower extract, vitamins, d-ribose, yeast β-glucan, yeast powder, and juice, add a small amount of water, stir to dissolve, and mix evenly; Add water to make up the mixed solution to volume, mix well, filter and fill; After heating and sterilization, elderberry and elderflower extract drink is obtained.

10. Use of the elderberry and elderflower extract drink as claimed in claim 1 in improving body immunity.

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