Grease pretreatment reagent and application thereof

Abstract

The invention discloses a grease pretreatment reagent and application thereof, and belongs to the technical field of grease treatment. The grease pretreatment reagent comprises the following components: a surfactant with a mass concentration of 1.5-3.5%, a neutral salt with a mass concentration of 3-7%, and an alkaline substance with a molar concentration of 0.003-0.018 mol / L. The surfactant in the grease pretreatment reagent provided by the invention can separate part of phospholipid and protein in a grease sample and reduce emulsification of the grease sample; the neutral salt can further separate a large amount of part of phospholipid and protein in the grease sample; the alkaline substance can remove impurities such as fatty acid in the grease sample; through the synergistic effect of the components, the grease pretreatment reagent can reduce emulsification of a grease sample and remove impurities, so that the grease sample treated by the grease pretreatment reagent can meet the requirement of rapid detection of capsaicin in grease, and therefore, the grease pretreatment reagent has a relatively good application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of grease treatment, and in particular relates to a grease pretreatment reagent and application thereof. Background Art

[0002] Oil is a high-energy source, with energy equivalent to 2.25 times that of carbohydrates and proteins. As an essential energy supply in livestock and poultry production, oil not only ensures the balance of energy and protein, but also has a variety of important physiological functions, such as acting as a solvent to promote the absorption of fat-soluble nutrients; providing a source of metabolic water in livestock and poultry; promoting the oil-water emulsification environment in the digestive tract; serving as a source of essential fatty acids in livestock and poultry, etc. Adding oil to feed can make it into high-energy feed. Driven by profit, feed oil will inevitably involve the problem of waste kitchen oil.

[0003] Waste cooking oil refers to all kinds of low-quality oils in life, such as recycled cooking oil, repeatedly used frying oil, etc. Long-term consumption may cause cancer and is extremely harmful to the human body. After washing, distillation, decolorization and other processing, or after mixing with other edible oils, it is difficult to distinguish waste cooking oil through sensory analysis and some physical and chemical indicators. Since 2011, the problem of waste cooking oil has become one of the most concerned food hot issues. The ultimate service object of animal husbandry is people's dining tables. The quality and safety of feed oils also need to be strictly controlled. The quality of the raw materials used is also the focus of the current food quality and safety system. A relatively accurate rapid screening method for waste cooking oil is of great value to strengthen supervision and management, ensure oil quality and people's health.

[0004] At present, there are many methods for identifying adulteration of waste cooking oil in edible oil, such as visible ultraviolet spectrometry, fluorescence analysis, magnetic resonance, colorimetry, which is the traditional method for detecting waste cooking oil. In addition, many new methods are also used for the identification of adulteration of waste cooking oil. For example, capsaicinoids can be used as exogenous markers of edible oil because of their strong fat solubility, good stability, and difficulty in being destroyed in oil. By detecting the content of capsaicinoids in feed oil samples, it can be determined whether the edible oil is adulterated with waste cooking oil, and the accuracy rate is more than 80%. Enzyme-linked immunosorbent assay, as a detection method with high specificity, high sensitivity, high accuracy, short time consumption, and easy operation, can be used to detect the content of capsaicinoids in oil. However, since most feed oils are animal and plant crude oils or mixed oils with complex components, emulsification will occur when extracting capsaicinoids from feed oils for rapid detection and analysis, resulting in a decrease in the extraction rate of capsaicinoids. At the same time, the impurities extracted will also interfere with the detection results.

[0005] Therefore, it is necessary to provide a fat pretreatment reagent that can pretreat the fat sample before sample extraction to remove some impurities with relatively large impacts to meet the requirements of rapid detection of capsaicin in feed fats and oils. Summary of the invention

[0006] The purpose of the present invention is to provide a fat pretreatment reagent and its application, which is used to solve the problems that when extracting capsaicin from feed fat for rapid detection and analysis, emulsification occurs, resulting in a reduced extraction rate of capsaicin, and impurities extracted from the extraction also interfere with the detection results.

[0007] In a first aspect, the present invention provides a grease pretreatment reagent, which includes the following components: a surfactant with a mass concentration of 1.5-3.5%, a neutral salt with a mass concentration of 3-7%, and an alkaline substance with a molar concentration of 0.003-0.018 mol / L.

[0008] In the oil pretreatment reagent provided by the present invention, the mass concentration of the surfactant can be, for example, 1.5%, 1.7%, 2%, 2.5%, 3%, 3.5% or other values ​​within this range; the mass concentration of the neutral salt can be, for example, 3%, 3.3%, 4%, 5%, 6%, 7% or other values ​​within this range; the molar concentration of the alkaline substance can be, for example, 0.003mol / L, 0.0033mol / L, 0.005mol / L, 0.008mol / L, 0.011mol / L, 0.015mol / L, 0.018mol / L or other values ​​within this range.

[0009] The oil pretreatment reagent provided by the present invention can effectively remove impurities such as fatty acids in oil samples, and can separate substances such as phospholipids to prevent emulsification of oil samples; wherein, the surfactant can separate part of the phospholipids and proteins in the oil samples to reduce the emulsification of the oil samples; the neutral salt can further separate part of the phospholipids and proteins in the oil samples in large quantities; the alkaline substance can remove impurities such as fatty acids in the oil samples, and the concentration of the alkaline substance needs to be controlled within a specific range. If the concentration of the alkaline substance is too low, the impurities are not completely removed, which interferes with the subsequent rapid detection of capsaicin in the oil; if the concentration of the alkaline substance is too high, part of the capsaicin in the oil sample will be removed, resulting in a low content of capsaicin in the subsequent rapid detection; through the synergistic effect of the above components, while reducing the emulsification of the oil sample, the impurities can also be removed, so that the oil sample treated with the oil pretreatment reagent can meet the requirements of the rapid detection of capsaicin in the oil.

[0010] In some embodiments, the oil pretreatment reagent includes the following components: a surfactant with a mass concentration of 1.7%, a neutral salt with a mass concentration of 3.3%, and an alkaline substance with a molar concentration of 0.0033 mol / L.

[0011] In some embodiments, the surfactant comprises PEG20000.

[0012] In the present invention, the inventors further studied and found that the surfactant PEG20000 can separate some phospholipids and proteins in the oil sample and reduce the emulsification of the oil sample.

[0013] In some embodiments, the neutral salt is selected from at least one of NaCl and KCl.

[0014] It is understandable that the neutral salt can be conventionally selected according to actual use requirements. In the present invention, the neutral salt is preferably selected from at least one of NaCl and KCl.

[0015] In some embodiments, the alkaline substance is selected from at least one of NaOH, NaHCO3, and Na2CO3.

[0016] It is understandable that the alkaline substance can be conventionally selected according to actual use needs. In the present invention, the alkaline substance is preferably selected from at least one of NaOH, NaHCO3, and Na2CO3.

[0017] In a second aspect, the present invention provides the use of any of the above-mentioned oil pretreatment reagents in the rapid detection of capsaicin content in oil samples.

[0018] In some embodiments, the above application includes the following steps: mixing the oil pretreatment reagent with the oil sample, shaking it, centrifuging it, and separating it to obtain the oil phase; using an enzyme-linked immunosorbent assay kit to detect the oil phase to obtain the capsaicin content in the oil sample.

[0019] In the present invention, when the grease pretreatment reagent is used to treat the grease sample, it has the advantages of simple operation and high impurity removal rate.

[0020] It is understandable that the enzyme-linked immunosorbent assay is a conventional quantitative detection method in the prior art. In the present invention, the enzyme-linked immunosorbent assay kit FS-01002 (Wuhan Food Safety Biotechnology Co., Ltd.) is preferably used for quantitative detection.

[0021] In some embodiments, during the separation and preparation of the oil phase, the oil sample includes a feed oil sample.

[0022] It can be understood that the fat sample includes conventional samples with complex components in the prior art, and the preferred fat sample in the present invention is a feed fat sample.

[0023] In some embodiments, during the separation and preparation process of the oil phase, the volume mass ratio of the oil pretreatment reagent and the oil sample is (2-3mL):1g, for example, it can be 2mL:1g, 2.2mL:1g, 2.4mL:1g, 2.6mL:1g, 2.8mL:1g, 3mL:1g or other ratios within this range; the oscillation treatment step specifically includes: the oscillation time is 30-120s, for example, it can be 30s, 60s, 90s, 120s or other values ​​within this range.

[0024] In the present invention, by controlling the volume mass ratio of the grease pretreatment reagent and the grease sample and the oscillation time within a specific range, impurities in the grease sample can be removed well.

[0025] It is understandable that the oscillation can be conventionally selected according to actual use needs, for example, it can be manual oscillation or oscillation using an oscillator. In the present invention, manual oscillation is preferred.

[0026] In some embodiments, during the separation and preparation of the oil phase, the centrifugation step specifically includes: centrifuging for 1-3 min, for example, 1 min, 2 min, 3 min or other values ​​within the range, at a rotation speed of 3000-5000 r / min (for example, 3000 r / min, 3500 r / min, 4000 r / min, 4500 r / min, 5000 r / min or other values ​​within the range).

[0027] The beneficial effects of the present invention are as follows: different from the prior art, the oil pretreatment reagent provided by the present invention can better remove impurities such as fatty acids in oil samples, and can separate substances such as phospholipids to prevent emulsification of oil samples; wherein the surfactant can separate part of the phospholipids and proteins in the oil samples to reduce the emulsification of the oil samples; the neutral salt can further separate part of the phospholipids and proteins in the oil samples in large quantities; the alkaline substance can remove impurities such as fatty acids in the oil samples; through the synergistic effect of the above components, while reducing the emulsification of the oil samples, impurities can also be removed, so that the oil samples treated with the oil pretreatment reagent can meet the requirements of rapid detection of capsaicin in oils, and therefore, have good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The results of treating feed fat with fat pretreatment reagent 1 in Example 1 of the present invention are shown in Figure 1, wherein (A) is a centrifugation result after pretreatment, and (B) is a centrifugation result before sample treatment; Figure 2 The results of treating feed fat with fat pretreatment reagent 2 in Example 1 of the present invention are shown in Figure 1, wherein (A) is a centrifugation result after pretreatment, and (B) is a centrifugation result before sample treatment; Figure 3 This is a graph showing the centrifugation result after pretreatment of feed oil using oil pretreatment reagent 3 in Example 1 of the present invention; Figure 4 The results of treating feed fat with fat pretreatment reagent 4 in Example 1 of the present invention are shown in Figure 1, wherein (A) is a centrifugation result after pretreatment, and (B) is a centrifugation result before sample treatment; Figure 5 The results of using the oil pretreatment reagent 4 to treat feed oil in Example 1 of the present invention are shown in Figure 1, wherein (A) is a graph showing the results of the pre-treatment centrifugation of the sample after pretreatment, and (B) is a graph showing the results of the pre-treatment centrifugation of the sample without pretreatment. DETAILED DESCRIPTION

[0029] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The experimental methods without specific conditions in the examples are usually carried out according to conventional conditions and conditions described in the manual, or according to conditions recommended by the manufacturer. The general equipment, materials, reagents, etc. used are all available from commercial channels unless otherwise specified.

[0031] In the present invention, the enzyme-linked immunosorbent assay kit FS-01002 is from Wuhan Food Safety Biotechnology Co., Ltd.

[0032] Example 1 Optimization test of oil pretreatment reagent components In this example, the effects of the surfactant, neutral salt and alkaline substance in the oil pretreatment reagent on treating feed oil separately are tested.

[0033] Specifically, PEG20000 aqueous solution with a mass concentration of 2%, Tween-20 aqueous solution with a mass concentration of 2%, PVPK30 aqueous solution with a mass concentration of 2%, NaCl aqueous solution with a mass concentration of 5%, KCl aqueous solution with a mass concentration of 5%, KCl aqueous solution with a mass concentration of 5%, NaOH aqueous solution with a molar concentration of 0.01 mol / L, NaHCO3 aqueous solution with a molar concentration of 0.01 mol / L, and Na2CO3 aqueous solution with a molar concentration of 0.01 mol / L were prepared respectively, 6 mL of the above aqueous solutions were added to different centrifuge tubes, and then 2 g of feed oil was added to the centrifuge tubes. After the centrifuge tubes were vigorously shaken up and down manually, the pretreatment phenomenon was observed, and then centrifuged at 4000 r / min for 1 min. The obtained oil phase was subjected to sample pretreatment by enzyme-linked immunosorbent assay kit FS-01002, and the sample pretreatment phenomenon was observed. The results are shown in Table 1.

[0034] Table 1 Effects of surfactants, neutral salts and alkaline substances on treating feed oils and fats

[0035] Among them, the turbidity of the upper layer of the pre-treatment of the kit samples in Table 1 indicates incomplete impurity removal, and the absence of floating objects on the surface indicates that the pre-treatment removes proteins and phospholipids relatively cleanly; the lack of saponification during pre-treatment indicates that free fatty acids are not bound.

[0036] As can be seen from Table 1, compared with other surfactants, PEG20000 can separate some proteins and phospholipids to a certain extent during the pretreatment process, reduce the emulsification in feed oil samples, and the supernatant is relatively clear, but it cannot remove free fatty acids in feed oils; neutral salts such as NaCl and KCl can separate phospholipids and some proteins in large quantities, but the supernatant is not clear enough and cannot remove free fatty acids in feed oils; compared with other alkaline substances, NaOH has better saponification ability and can better remove free fatty acids in feed oil samples, but the samples will emulsify and cannot remove other impurities.

[0037] Since the effects of treating feed fats and oils with surfactants, neutral salts and alkaline substances alone are not good, a combination of surfactants, neutral salts and alkaline substances is further used to treat feed fats and oils.

[0038] Specifically, a grease pretreatment reagent 1 containing 2% PEG20000 and 5% NaCl, a grease pretreatment reagent 2 containing 2% PEG20000 and 0.01 mol / L NaOH, a grease pretreatment reagent 3 containing 5% NaCl and 0.01 mol / L NaOH, and a grease pretreatment reagent 4 containing 2% PEG20000, 5% NaCl and 0.01 mol / L NaOH were prepared respectively, 6 mL of the above grease pretreatment reagents were added to different centrifuge tubes, and then 2 g of feed oil was added to the centrifuge tubes. After manually shaking the centrifuge tubes up and down vigorously, the pretreatment phenomenon was observed, and then centrifuged at 4000 r / min for 1 min. The obtained oil phase was subjected to sample pretreatment using enzyme-linked immunosorbent assay kit FS-01002, and the sample pretreatment phenomenon was observed. The results are as follows: Figure 1-4 and as shown in Table 2 below.

[0039] Table 2 Effect of PEG20000, NaCl and NaOH combined treatment of feed oil

[0040] Among them, the turbidity of the upper layer of the pre-treatment of the kit samples in Table 2 indicates incomplete impurity removal, and the absence of floating objects on the surface indicates that the pre-treatment has removed proteins and phospholipids relatively cleanly; the lack of saponification during pre-treatment indicates that the free fatty acids are not combined, and severe saponification indicates that a sufficient oil layer cannot be separated.

[0041] from Figure 1-4 As can be seen from Table 2, the combined oil pretreatment reagent of PEG20000 and NaCl cannot remove free fatty acids in feed oil samples when treating feed oil samples; the combined oil pretreatment reagent of PEG20000 and NaOH cannot remove a large amount of phospholipids and proteins in feed oil samples when treating feed oil samples; the combined oil pretreatment reagent of NaCl and NaOH causes serious saponification of the samples when treating feed oil samples, and it is impossible to separate a sufficient amount of oil layer for the next step, resulting in serious sample waste; while the combined oil pretreatment reagent of PEG20000, NaCl and NaOH can simultaneously remove free fatty acids, phospholipids and proteins in feed oil samples when treating feed oil samples, and can also ensure that no emulsification occurs during subsequent sample pretreatment.

[0042] Further, 6 mL of the above-mentioned oil pretreatment reagent 4 was added to a centrifuge tube, and then 2 g of feed oil was added to the centrifuge tube. After the centrifuge tube was manually shaken up and down vigorously, the pretreatment phenomenon was observed, and then centrifuged at 4000 r / min for 1 min. The obtained oil phase was pretreated with an enzyme-linked immunosorbent assay kit FS-01002, and the sample pretreatment phenomenon was observed. The sample that was not pretreated with the oil pretreatment reagent 4 was used as a control. The results are as follows: Figure 5 shown.

[0043] from Figure 5 It can be seen that compared with samples that have not been pretreated with the oil pretreatment reagent 4, the combined oil pretreatment reagent 4 using PEG20000, NaCl and NaOH can simultaneously remove free fatty acids, phospholipids and proteins in the feed oil samples when treating the feed oil samples, and can also ensure that no emulsification occurs during subsequent sample pretreatment.

[0044] Example 2 Experiment on optimizing the concentration of components of oil pretreatment reagents In this example, the concentrations of the surfactant, the neutral salt and the alkaline substance in the oil pretreatment reagent optimized in Example 1 were optimized and tested.

[0045] Specifically, prepare the following grease pretreatment reagent: Oil pretreatment reagent A: containing PEG20000 with a mass concentration of 0.7%, NaCl with a mass concentration of 1.7% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent B: containing PEG20000 with a mass concentration of 1.7%, NaCl with a mass concentration of 1.7%, and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent C: containing PEG20000 with a mass concentration of 3.3%, NaCl with a mass concentration of 1.7% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent D: containing PEG20000 with a mass concentration of 0.7%, NaCl with a mass concentration of 3.3% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent E: containing PEG20000 with a mass concentration of 1.7%, NaCl with a mass concentration of 3.3% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent F: containing PEG20000 with a mass concentration of 3.3%, NaCl with a mass concentration of 3.3%, and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent G: containing PEG20000 with a mass concentration of 0.7%, NaCl with a mass concentration of 6.7% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent H: containing PEG20000 with a mass concentration of 1.7%, NaCl with a mass concentration of 6.7% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent I: containing PEG20000 with a mass concentration of 3.3%, NaCl with a mass concentration of 6.7% and NaOH with a molar concentration of 0.0033 mol / L; Oil pretreatment reagent J: containing PEG20000 with a mass concentration of 0.7%, NaCl with a mass concentration of 1.7% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent K: containing PEG20000 with a mass concentration of 1.7%, NaCl with a mass concentration of 1.7%, and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent L: containing PEG20000 with a mass concentration of 3.3%, NaCl with a mass concentration of 1.7% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent M: containing PEG20000 with a mass concentration of 0.7%, NaCl with a mass concentration of 3.3% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent N: containing PEG20000 with a mass concentration of 1.7%, NaCl with a mass concentration of 3.3% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent O: containing PEG20000 with a mass concentration of 3.3%, NaCl with a mass concentration of 3.3% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent P: containing PEG20000 with a mass concentration of 0.7%, NaCl with a mass concentration of 6.7% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent Q: containing PEG20000 with a mass concentration of 1.7%, NaCl with a mass concentration of 6.7% and NaOH with a molar concentration of 0.03 mol / L; Oil pretreatment reagent R: contains PEG20000 with a mass concentration of 3.3%, NaCl with a mass concentration of 6.7% and NaOH with a molar concentration of 0.03 mol / L.

[0046] 6 mL of the above-mentioned oil pretreatment reagent was added to different centrifuge tubes respectively, and then 2 g of feed oil (capsaicin content was 5 μg / kg) was added to the centrifuge tubes. The centrifuge tubes were manually shaken up and down vigorously, and then centrifuged at 4000 r / min for 1 min. The obtained oil phase was pre-treated by enzyme-linked immunosorbent assay kit FS-01002, and then the capsaicin content was detected and the recovery rate was calculated. The results are shown in Table 3 below.

[0047] Table 3 Optimization results of component concentrations of oil pretreatment reagents

[0048] It can be seen from Table 3 that when the mass concentrations of PEG20000 and NaCl in the oil pretreatment reagent are low, the sample will emulsify and cannot be detected; when the molar concentration of NaOH is high, the recovery rate of capsaicin in the sample will be reduced. Therefore, in the oil pretreatment reagent provided by the present invention, when the mass concentration of the surfactant is 1.5-3.5%, the mass concentration of the neutral salt is 3-7%, and the molar concentration of the alkaline substance is 0.003-0.018 mol / L, it has a good pretreatment effect on the feed oil sample, so that the recovery rate of capsaicin in the sample is higher; when the mass concentration of the surfactant in the oil pretreatment reagent is 1.7%, the mass concentration of the neutral salt is 3.3%, and the molar concentration of the alkaline substance is 0.0033 mol / L, it has the best effect.

[0049] Example 3 Application of oil pretreatment reagent in rapid detection of capsaicin content in oil samples First, the volume-to-mass ratio of the oil pretreatment reagent and the oil sample was optimized. Specifically, the oil pretreatment reagent E (2, 4, and 6 mL) prepared in Example 2 was added to different centrifuge tubes, and then 2 g of feed oil (capsaicin content was 5 μg / kg) was added to the centrifuge tube. The centrifuge tube was manually shaken up and down vigorously, and then centrifuged at 4000 r / min for 1 min. The obtained oil phase was pre-treated with an enzyme-linked immunosorbent assay kit FS-01002, and then the capsaicin content was detected and the recovery rate was calculated. The results are shown in Table 4 below.

[0050] Table 4 Optimization results of the volume-to-mass ratio of the oil pretreatment reagent and the oil sample

[0051] It can be seen from Table 4 that the volume mass ratio of the oil pretreatment reagent and the oil sample is 6mL:2g, and the sample has a good recovery rate.

[0052] Next, the oscillation time after the oil pretreatment reagent and the oil sample were mixed was optimized. Specifically, 6 mL of the oil pretreatment reagent E prepared in Example 2 was added to different centrifuge tubes, and then 2 g of feed oil (capsaicin content was 5 μg / kg) was added to the centrifuge tubes. The centrifuge tubes were manually shaken up and down vigorously for different times (10, 30, 60, 120 s), and then centrifuged at 4000 r / min for 1 min. The obtained oil phase was pre-treated with an enzyme-linked immunosorbent assay kit FS-01002, and then the capsaicin content was detected and the recovery rate was calculated. The results are shown in Table 5 below.

[0053] Table 5 Pretreatment oscillation time optimization results

[0054] It can be seen from Table 5 that after the oil pretreatment reagent was mixed with the oil sample and oscillated for 60 seconds, the sample had a good recovery rate.

[0055] Finally, the ability of the oil pretreatment reagent to treat feed oil was tested. Specifically, 6 mL of the oil pretreatment reagent E prepared in Example 2 was added to different centrifuge tubes, and then 2 g of feed oil samples containing different concentrations of capsaicin (7 samples) were added to the centrifuge tubes respectively. The centrifuge tubes were manually shaken up and down vigorously for 60 seconds, and then centrifuged at 4000 r / min for 1 minute. Three parallels were used for each sample. The obtained oil phase was pre-treated with an enzyme-linked immunosorbent assay kit FS-01002, and then the capsaicin content was detected. The recovery rate was calculated and compared with the results of directly using the enzyme-linked immunosorbent assay kit FS-01002 to detect the 7 feed oil samples. The results are shown in Table 6 below.

[0056] Table 6 Test results of 7 feed oil samples

[0057] It can be seen from Table 6 that if the sample is not treated with the oil pretreatment reagent of the present invention, emulsification will occur and the capsaicin content cannot be detected. At the same time, the error of the detected capsaicin content is extremely large. After being pretreated with the oil pretreatment reagent of the present invention, the sample does not emulsify, and the capsaicin content is quantified more accurately.

[0058] In summary, the oil pretreatment reagent provided by the present invention can effectively remove impurities such as fatty acids in oil samples, and can separate substances such as phospholipids to prevent emulsification of oil samples, so that the oil samples treated with the oil pretreatment reagent can meet the requirements of rapid detection of capsaicin in oil.

[0059] It should be noted that the above embodiments all belong to the same inventive concept, and the description of each embodiment has its own focus. For matters that are not described in detail in some embodiments, reference may be made to the description in other embodiments.

[0060] The above-mentioned embodiments only express the implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A grease pretreatment reagent, characterized in that: The oil pretreatment reagent comprises the following components: a surfactant with a mass concentration of 1.5-3.5%, a neutral salt with a mass concentration of 3-7%, and an alkaline substance with a molar concentration of 0.003-0.018 mol / L.

2. The grease pretreatment reagent according to claim 1, characterized in that: The oil pretreatment reagent comprises the following components: a surfactant with a mass concentration of 1.7%, a neutral salt with a mass concentration of 3.3%, and an alkaline substance with a molar concentration of 0.0033 mol / L.

3. The grease pretreatment reagent according to claim 1, characterized in that: The surfactant includes PEG20000.

4. The grease pretreatment reagent according to claim 1, characterized in that: The neutral salt is selected from at least one of NaCl and KCl.

5. The grease pretreatment reagent according to claim 1, characterized in that: The alkaline substance is selected from at least one of NaOH, NaHCO3, and Na2CO3.

6. Use of the oil pretreatment reagent according to any one of claims 1 to 5 in the rapid detection of capsaicin content in oil samples.

7. The use according to claim 6, characterized in that: The steps include: The oil pretreatment reagent is mixed with the oil sample, and after shaking, centrifugation is performed to separate the oil phase; The oil phase is detected using an enzyme-linked immunosorbent assay kit to obtain the content of capsaicin in the oil sample.

8. The use according to claim 7, characterized in that: In the separation and preparation process of the oil phase, the oil sample includes a feed oil sample.

9. The use according to claim 7, characterized in that: In the separation and preparation process of the oil phase, the volume mass ratio of the oil pretreatment reagent and the oil sample is (2-3mL):1g; The oscillation treatment step specifically includes: the oscillation time is 30-120s.

10. The use according to claim 7, characterized in that: In the separation and preparation process of the oil phase, the centrifugation step specifically includes: centrifuging for 1-3 minutes at a rotation speed of 3000-5000 r / min.

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