Immunity-enhancing and anti-fatigue solid beverage and preparation process thereof

Abstract

The invention belongs to the technical field of solid beverage processing, and particularly relates to a solid beverage capable of enhancing immunity and resisting fatigue and a preparation technology of the solid beverage. The solid beverage is prepared from the following raw materials: 56-60 parts of morchella mycelium powder, 5-7 parts of catathelasma ventricosum peptide powder, 5-7 parts of ginseng peptide powder, 5-7 parts of black sesame peptide powder, 5-7 parts of Chinese wolfberry peptide powder, 5-7 parts of dendrobium officinale peptide, 5-7 parts of soybean peptide and 5-7 parts of black bean peptide powder. High-quality wheat is used as a main component of a culture medium, high-quality morchella hyphae are successfully cultivated and made into powder, the powder is scientifically matched with short peptides such as soybean peptide and dendrobium officinale peptide, a novel solid beverage is developed, and through rich nutritional ingredients and bioactive substances of the morchella hyphae, the functions of intestines and stomach are effectively conditioned, kidney health is enhanced, and the health of a human body is promoted. The human body immunity is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of solid beverage processing, and in particular relates to an immunity-enhancing and fatigue-resistant solid beverage and a preparation process thereof. Background Art

[0002] As the pace of modern life accelerates, people are facing increasing pressure, and the number of people in sub-healthy state is increasing. Sub-healthy state is mainly manifested in problems such as decreased immunity, fatigue, gastrointestinal disorders and kidney dysfunction. In order to improve these conditions, various functional foods and health products have appeared on the market, among which solid beverages are widely welcomed because of their convenience in carrying and drinking.

[0003] Existing functional solid beverages mostly rely on a single ingredient, such as vitamin C, vitamin E, etc. Although they can supplement certain nutrients in the short term, they have poor long-term effects and are difficult to comprehensively improve a variety of health problems in the human body. In addition, the formulas of many functional beverages are not scientific enough to fully utilize the synergistic effects of the various ingredients, resulting in unsatisfactory results.

[0004] In view of the above technical problems, the present invention proposes an immunity-enhancing, anti-fatigue solid beverage and its preparation process to overcome the deficiencies in the prior art, effectively regulate gastrointestinal function, enhance kidney health, and improve human immunity. Summary of the invention

[0005] The purpose of the present invention is to provide a solid beverage for enhancing immunity and resisting fatigue and a preparation process thereof. Morel mycelium powder is scientifically proportioned with short peptides such as soybean peptide and Dendrobium officinale peptide to develop a new type of solid beverage. Through its rich nutrients and bioactive substances, it can effectively regulate gastrointestinal function, enhance kidney health and improve human immunity.

[0006] A solid beverage for enhancing immunity and resisting fatigue. The raw materials for its preparation include, by weight, 56-60 parts of morel mycelium powder, 5-7 parts of mulberry peptide powder, 5-7 parts of ginseng peptide powder, 5-7 parts of black sesame peptide powder, 5-7 parts of wolfberry peptide powder, 5-7 parts of dendrobium officinale peptide, 5-7 parts of soybean peptide and 5-7 parts of black bean peptide powder.

[0007] The inventors found that the use of specific weight portions of Morchella mycelium powder, old man's head peptide powder, ginseng peptide powder, black sesame peptide powder, wolfberry peptide powder, Dendrobium officinale peptide, soybean peptide, and black bean peptide powder as raw materials for solid beverages can effectively regulate gastrointestinal function, enhance kidney health, and improve human immunity. This may be because these raw materials work synergistically. Morchella mycelium powder, as the main ingredient of the solid beverage, contains rich polysaccharides, organic germanium, and a variety of amino acids and minerals. These ingredients help to enhance immunity, anti-fatigue, and anti-virus, and have a certain help in gastric mucosal repair. At the same time, it can promote gastrointestinal motility and improve constipation symptoms. Old man's head peptide powder is rich in protein, polysaccharides, minerals, and vitamins, providing comprehensive nutritional support for the human body, helping to enhance immunity and anti-fatigue. Ginseng polysaccharides in ginseng peptide powder have the effect of stimulating the proliferation and activation of immune cells, thereby improving the body's resistance to pathogens. Black sesame peptide powder and wolfberry peptide powder are rich in a variety of antioxidants, which help to delay aging and enhance body resistance. Dendrobium officinale has the effects of nourishing yin and clearing heat, benefiting the stomach and promoting fluid, moistening the lungs and relieving cough. The polysaccharides and amino acids it contains can enhance immunity, promote the secretion of digestive juices, and help digestion. Soy peptides and black bean peptide powders are rich in protein and a variety of amino acids, which help repair body tissues and improve body functions. On the one hand, these raw materials jointly provide rich nutritional support for the human body, including protein, polysaccharides, amino acids, minerals and vitamins, which are necessary to maintain normal physiological functions of the human body. On the other hand, they act on the human body through different mechanisms of action, such as enhancing immunity, promoting digestion, and anti-oxidation, so as to regulate gastrointestinal function, enhance kidney health and enhance human immunity. For example, the polysaccharide components in morel mycelium powder and old man's head peptide powder can stimulate the proliferation and activation of immune cells and improve the body's resistance to pathogens; while Dendrobium officinale peptides, soybean peptides, and black bean peptide powders help improve gastrointestinal function and enhance body functions by promoting the secretion of digestive juices and repairing body tissues. Therefore, the various raw materials in the solid beverage work together to regulate gastrointestinal function, enhance kidney health and improve human immunity, providing comprehensive protection for human health. In order to maximize the beneficial effects of solid beverages, it is necessary to ensure that the effective ingredients of each group of minutes in the raw materials are as much as possible.

[0008] The preparation method of the morel mycelium powder comprises the following steps:

[0009] S1. Preparation of stock: inoculate the mother stock of Morchella into the culture medium and culture it at 23-25°C for 45-60 days to obtain the stock;

[0010] S2. Cultivated seed production: the original seed is transferred into a sterilized original seed nutrient bag and cultured at 23-25°C for 25-30 days to obtain cultivated seeds;

[0011] S3, cultivation: the cultivation seeds are placed in sterilized cultivation seed nutrient bags and cultured at 23-25° C. for 25-30 days, the seeds are sown and planted, and the temperature in the greenhouse is controlled at 23-25° C. After culturing for 25-30 days, an external aid nutrient bag is added, and the culture is continued for 25-30 days, and the external aid nutrient bag is removed to obtain Morchella mycelium;

[0012] S4, collecting Morchella mycelia, drying, dehydrating, and grinding to obtain Morchella mycelia powder.

[0013] The mother strain of morel is the Liumei morel variety. It is obtained by selecting high-quality mushrooms from the field and then naturally air-drying them, and then undergoing tissue separation, cultivation and purification. It comes from Mianyang Jingke Fungi Industry Co., Ltd.

[0014] Preferably, the inoculation amount of the Morchella mother culture is 5%-15% of the mass of the culture medium.

[0015] The culture medium is prepared from raw materials including a carbon source, a nitrogen source, water and a pH regulator; the pH value of the culture medium is 6.0-6.5.

[0016] Preferably, the mass of the carbon source in the culture medium is 4%-6% of the mass of water; the mass of the nitrogen source is 0.5%-1% of the mass of water.

[0017] The carbon source includes one or more of glucose, mannitol, corn flour, fructose, sucrose, and maltose; more preferably, glucose, corn flour, and fructose.

[0018] Preferably, the mass ratio of glucose, corn flour and fructose is 1:(1-2):(1-2); further preferably, it is 2:3:3.

[0019] Preferably, the nitrogen source includes one or more of soybean powder, peptone, yeast powder, potassium nitrate, and ammonium sulfate; further preferably, it is soybean powder, peptone, and yeast powder.

[0020] Preferably, the mass ratio of soybean powder, peptone and yeast powder is (8-12):1:(1-2); further preferably, it is 20:2:3.

[0021] The nutrient formulas in the original seed nutrient bag and the cultivated seed nutrient bag are consistent by weight, including 50-60 parts of wheat, 15-20 parts of wheat bran, 5-10 parts of sawdust, 3-5 parts of rice husks, and 0.5-1 part of lime.

[0022] Calculated by weight, the nutrients in the foreign aid nutrient bag include 50-60 parts of wheat, 15-20 parts of wheat bran, 10-15 parts of corn flour, 5-10 parts of soybean meal, and 0.5-1 part of lime.

[0023] The inventors found that by treating Morchella in a specific way, not only the yield of Morchella mycelium can be increased, but also the nutritional value and quality of Morchella mycelium can be improved. This may be because by culturing in stages and adding external aid nutrient bags, the adaptability of the strain to the environment can be improved, and additional nutritional support can be provided for the mycelium according to the growth of the mycelium to meet the special needs of its growth process, thereby significantly improving the yield and quality of the mycelium, making the Morchella product more nutritious and better in taste. Wheat and wheat bran in the original seed nutrient bag and the cultivated seed nutrient bag are the main carbon and nitrogen sources, providing the necessary energy and material basis for the growth of Morchella mycelium, and the nutrients they are rich in are easily absorbed and utilized by the mycelium, promoting the rapid growth and reproduction of the mycelium. At the same time, the addition of sawdust and rice husks not only increases the air permeability and water retention of the culture medium, but also provides the trace elements and minerals required for the growth of mycelium, which helps the mycelium to form a robust mycelium and a rich mycelium network. Corn flour and soybean meal, two high-quality nitrogen sources, are specially added to the foreign aid nutrient bag. Corn flour is rich in starch and multiple amino acids, which provides abundant energy and nitrogen nutrition for the mycelium and promotes the robust growth of the mycelium. Soybean meal is a high-protein, high-fat raw material that is rich in essential amino acids and fatty acids. These ingredients are of great significance for improving the quality and yield of morel mycelium. By adding these two raw materials, the formula of the foreign aid nutrient bag is more comprehensive and can better meet the special needs of morels during their growth. In addition, the addition of lime also plays an important role in regulating the pH of the culture medium, inhibiting the growth of miscellaneous bacteria, and promoting the development of mycelium. The appropriate pH environment is conducive to the growth and reproduction of morel mycelium, and the addition of lime can effectively control the pH of the culture medium and provide a good environment for mycelium growth. Through carefully designed culture steps and nutrient ratios, the growth environment of morel mycelium can be optimized, which helps to reduce the contamination and competition of miscellaneous bacteria and improve the purity and quality of mycelium.

[0024] In some preferred schemes, when mixing the various nutrients, the wheat needs to be soaked in lime water in advance until there is no white core, and the mixture is sterilized and then mixed with other raw materials.

[0025] The preparation methods of the tilapia peptide powder, ginseng peptide powder, wolfberry peptide powder and dendrobium officinale peptide are the same, and the preparation raw materials are tilapia, ginseng, wolfberry and dendrobium officinale respectively.

[0026] Taking the old man's head peptide powder as an example, its preparation method comprises the following steps:

[0027] A1. Wash the old man's head mushroom, slice it, dry it until the water content is less than 10%, crush it and put it into a fermentation tank, add water to adjust the water content to 45% to 55% to obtain a fermentation substrate, add a fermentation agent, ferment at 40 to 45° C. until the pH is 4 to 4.5, take it out, dehydrate it and concentrate it to obtain a fermentation product;

[0028] A2, extracting the fermented product with 65% to 75% ethanol solution twice under reflux, each time for 1 to 2 hours, filtering, combining the two extracts to obtain a Psoralea corylifolia extract;

[0029] A3. Adjust the pH of the extract of Agaricus chinensis to 8-9, add alkaline protease, react at 50-60°C for 1-3 hours, and then boil and inactivate. Adjust the pH of the system to 7-8, add trypsin, react at 37-40°C for 1-3 hours, and then boil and inactivate. Adjust the pH of the system to 6-7, add papain, react at 50-55°C for 2-4 hours, and then boil and inactivate. Adjust the pH of the system to 2-3, add pepsin, react at 37-40°C for 2-4 hours, and then boil and inactivate. Cool to room temperature, centrifuge, take the supernatant, pass through an ultrafiltration membrane, and concentrate and spray-dry to obtain.

[0030] Preferably, the fermentation agent includes Candida lipolytica, Bacillus subtilis, and Lactobacillus acidophilus.

[0031] The strain number of the Candida lipolytica is CI CC 32245; the strain number of the Bacillus subtilis is ATCC 6633; and the strain number of the Lactobacillus acidophilus is CI CC 6091.

[0032] Preferably, the dosage of the Candida lipolytica is 10 8 -10 9 CFU / g fermentation substrate; the amount of Bacillus subtilis is 10 7 -10 8 CFU / g fermentation substrate; the amount of Lactobacillus acidophilus is 10 6 ~10 7 CFU / g fermentation substrate.

[0033] Preferably, the addition amount of the alkaline protease is 50-100 U / g of Glechoma longituba, the addition amount of the trypsin is 500-1000 U / g of Glechoma longituba, the addition amount of the papain is 800-1500 U / g of Glechoma longituba, and the addition amount of the pepsin is 500-1000 U / g of Glechoma longituba.

[0034] The ultrafiltration membrane has a molecular weight cutoff of 1 kDa and was purchased from Beijing Zeping Biotechnology Co., Ltd.

[0035] The inventors found that by first fermenting the old man's head mushroom and then obtaining the old man's head mushroom peptide powder by step-by-step enzymolysis, the content of short peptides in the old man's head mushroom peptide powder can be increased while increasing the content of other beneficial ingredients such as polysaccharides and vitamins. This may be because first in the fermentation stage, specific fermentation agents Candida lipolytica, Bacillus subtilis and Lactobacillus acidophilus are selected. These three fermentation agents work synergistically to decompose the complex components in the old man's head mushroom and release small molecules that are easier to be absorbed and utilized by the human body. Candida lipolytica has a strong fat decomposition ability and can help decompose the lipid components in the old man's head mushroom to produce more fatty acids and flavor substances; while Bacillus subtilis and Lactobacillus acidophilus can decompose proteins and polysaccharides to produce beneficial ingredients such as amino acids and oligosaccharides. The synergistic effect of these microorganisms not only provides a richer substrate for the subsequent enzymolysis process, but also promotes the release and conversion of beneficial ingredients in the old man's head mushroom. Secondly, in the step-by-step enzymatic hydrolysis stage, by adding alkaline protease, trypsin, papain and pepsin in sequence, these enzymes can gradually decompose the fermented old man's head mushroom into short peptides, amino acids and other small molecule compounds under their respective optimal temperature and pH conditions. Alkaline protease first acts on the protein in the fermentation product to hydrolyze it into larger peptides; then, trypsin further hydrolyzes these peptides into smaller peptides and amino acids; papain can specifically hydrolyze the peptide bonds in certain proteins to produce specific active peptides; finally, pepsin continues to hydrolyze the remaining proteins and polypeptides under acidic conditions to ensure that the final product has a high content of short peptides and is easy to absorb. This step-by-step enzymatic hydrolysis process not only improves the yield and purity of short peptides, but also retains the beneficial ingredients such as polysaccharides and vitamins in old man's head mushrooms, realizing the synergistic extraction and optimization of nutrients. Finally, the content of short peptides was further increased by filtration through ultrafiltration membrane.

[0036] The preparation methods of the black sesame peptide powder, soybean peptide and black soybean peptide powder are the same, and the preparation raw materials are black sesame meal, soybean meal and black soybean meal respectively.

[0037] Taking black sesame peptide powder as an example, its preparation method includes the following steps: mixing black sesame meal and glucose, adding water to adjust the water content to 60%-70%, adjusting the pH to 5.5-6.5, sterilizing and cooling to obtain a culture medium; inoculating mold into the culture medium, fermenting at 25-35°C for 3-5 days, passing through an ultrafiltration membrane and freeze-drying to obtain black sesame peptide powder.

[0038] Preferably, the added amount of glucose is 5% to 10% of the mass of the black sesame meal.

[0039] Preferably, the inoculation amount of the mold is 8%-12%; further preferably, it is 10%.

[0040] Preferably, the mold includes Aspergillus niger, Aspergillus oryzae, and Monascus purpurogenum.

[0041] Preferably, the mass ratio of Aspergillus niger, Aspergillus oryzae and Monascus purpurogenum is 1:(0.5-2):(0.5-2); further preferably, it is 1:1:1.

[0042] The Aspergillus niger AS3.350 and Aspergillus oryzae A-9005 were purchased from the Microbiology Laboratory of Shandong University.

[0043] The Monascus purpureus was purchased from the Biological and Medical Laboratory of Fujian Normal University.

[0044] The ultrafiltration membrane has a molecular weight cutoff of 1 kDa and was purchased from Beijing Zeping Biotechnology Co., Ltd.

[0045] The inventors found that by using specific molds to ferment black sesame meal, not only most of the nutrients in black sesame, such as vitamin E, lecithin, calcium, iron, etc., are retained, but also these nutrients are converted into a form that is easier to be absorbed and utilized by the human body through the synergistic effect of molds. During the fermentation process, Aspergillus niger, Aspergillus oryzae and Monascus purpurogenus work together to secrete a variety of enzymes such as protease, amylase, lipase, etc., which efficiently decompose macromolecules such as protein, starch and fat in black sesame meal, and produce small molecule nutrients such as short peptides, amino acids, and glucose. At the same time, there is a synergistic effect between the three molds, which promote the growth and production of metabolites of each other, making the fermentation process more efficient and stable. In addition, molds can also adjust the pH value and temperature of the fermentation environment, inhibit the growth of harmful microorganisms, and promote the growth of beneficial microorganisms and the production of metabolites. Finally, after ultrafiltration membrane filtration and freeze-drying, the obtained black sesame peptide powder is not only pure and free of impurities, but also has a unique aroma and taste, which is more mellow and rich. More importantly, the short peptides and polypeptides produced by fermentation have multiple health functions such as anti-oxidation, lowering blood lipids and blood sugar, and have a positive effect on physical health. Therefore, the black sesame peptide powder prepared by this method is not only high in nutritional value, but also easy to digest and absorb, and has broad market prospects and application value.

[0046] The preparation process of the solid beverage for enhancing immunity and resisting fatigue comprises the following steps: mixing the raw materials uniformly and then sealing and packaging the mixture.

[0047] The solid beverage can be used as a health product, a medicine or an edible product.

[0048] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0049] 1. The present invention provides a solid beverage for enhancing immunity and resisting fatigue. High-quality wheat is used as the main component of the culture medium, and high-quality morel mycelium is successfully cultivated and made into powder. It is scientifically proportioned with short peptides such as soybean peptide and Dendrobium officinale peptide to develop a new type of solid beverage. Through its rich nutrients and bioactive substances, it can effectively regulate gastrointestinal function, enhance kidney health, and improve human immunity.

[0050] 2. The present invention can effectively regulate gastrointestinal function, enhance kidney health and improve human immunity by selecting specific weight portions of morel mycelium powder, old man's head peptide powder, ginseng peptide powder, black sesame peptide powder, wolfberry peptide powder, dendrobium officinale peptide, soybean peptide and black bean peptide powder as raw materials for solid beverages.

[0051] 3. The present invention processes Morchella in a specific manner, which can not only increase the yield of Morchella mycelium, but also improve the nutritional value and quality of Morchella mycelium.

[0052] 4. The present invention first ferments the old man's head mushroom and then obtains the old man's head mushroom peptide powder by step-by-step enzymolysis, which can increase the content of short peptides in the old man's head mushroom peptide powder while increasing the content of other beneficial ingredients such as polysaccharides and vitamins.

[0053] 5. The present invention uses specific molds to ferment black sesame meal, which not only retains most of the nutrients in black sesame, such as vitamin E, lecithin, calcium, iron, etc., but also converts these nutrients into a form that is easier to be absorbed and utilized by the human body through the synergistic effect of the molds. DETAILED DESCRIPTION

[0054] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0055] The raw materials described in the present invention are all commercially available, and are specifically as follows:

[0056] Morel mother culture, Mianyang Jingke Fungi Industry Co., Ltd.

[0057] Corn flour, protein content ≥90%, was purchased from Shaanxi Yihaochuan Biotechnology Co., Ltd.

[0058] Soybean powder, with a soybean isoflavone content of ≥80%, was purchased from Shaanxi Junhe Biotechnology Co., Ltd.

[0059] Peptone, with active ingredient content ≥99% and total colony count ≤1000 CFU / g, was purchased from Zhejiang Yicun Biotechnology Co., Ltd.

[0060] Yeast powder, with a crude protein content of ≥40% and a cell count of ≥27 billion / g, was purchased from Wuhan Mengqi Technology Co., Ltd.

[0061] Black sesame meal, soybean meal, and black soybean meal were purchased from Huamo (Shandong) Biotechnology Co., Ltd.

[0062] Candida lipolytica, strain number CI CC 32245; Bacillus subtilis, strain number ATCC6633; Lactobacillus acidophilus, strain number CI CC 6091.

[0063] Papain, enzyme activity of about 100,000 U / g; trypsin, enzyme activity of about 200,000 U / g; alkaline protease, enzyme activity of about 200,000 U / g; pepsin, enzyme activity of about 12,000 U / g; all were purchased from Ningxia Xiasheng Industrial Group Co., Ltd.

[0064] The ultrafiltration membrane had a molecular weight cutoff of 1 kDa and was purchased from Beijing Zeping Biotechnology Co., Ltd.

[0065] Aspergillus niger AS3.350 and Aspergillus oryzae A-9005 were purchased from the Microbiology Laboratory of Shandong University.

[0066] Monascus purpureus was purchased from the Biological and Pharmaceutical Laboratory of Fujian Normal University.

[0067] Example 1

[0068] The present embodiment provides a solid beverage for enhancing immunity and resisting fatigue. The raw materials for its preparation are, by weight, 58 parts of morel mycelium powder, 6 parts of tilapia peptide powder, 6 parts of ginseng peptide powder, 6 parts of black sesame peptide powder, 6 parts of wolfberry peptide powder, 6 parts of dendrobium officinale peptide, 6 parts of soybean peptide, and 6 parts of black soybean peptide powder.

[0069] The preparation method of the morel mycelium powder comprises the following steps:

[0070] S1. Preparation of stock: inoculate the mother strain of Morchella into the culture medium and culture it at 25°C for 50 days to obtain the stock;

[0071] S2. Cultivated seed preparation: the original seed is transferred into a sterilized original seed nutrient bag and cultured at 25° C. for 30 days to obtain cultivated seeds;

[0072] S3, cultivation: the cultivation seeds are placed in sterilized cultivation seed nutrient bags and cultured at 25°C for 30 days, the seeds are sown and planted, and the temperature in the greenhouse is controlled at 25°C. After culturing for 30 days, an external aid nutrient bag is added, and the culture is continued for 30 days, and the external aid nutrient bag is removed to obtain Morchella mycelium;

[0073] S4, collecting Morchella mycelia, drying, dehydrating, and grinding to obtain Morchella mycelia powder.

[0074] The inoculation amount of the Morchella mother strain is 10% of the mass of the culture medium.

[0075] The culture medium is prepared from raw materials including a carbon source, a nitrogen source, water and a pH regulator; the pH value of the culture medium is adjusted to 6.5 by adding the pH regulator.

[0076] The mass of the carbon source in the culture medium is 5% of the mass of water; the mass of the nitrogen source is 0.8% of the mass of water.

[0077] The carbon sources are glucose, corn flour and fructose in a mass ratio of 2:3:3.

[0078] The nitrogen source is soybean powder, peptone and yeast powder, and the mass ratio is 20:2:3.

[0079] The nutrient formulas in the original seed nutrient bag and the cultivated seed nutrient bag are consistent by weight, namely, 55 parts of wheat, 18 parts of wheat bran, 8 parts of sawdust, 4 parts of rice husks and 1 part of lime.

[0080] Measured by weight, the nutrients in the foreign aid nutrition bag include 55 parts of wheat, 18 parts of wheat bran, 12 parts of corn flour, 8 parts of soybean meal, and 1 part of lime.

[0081] When mixing the various nutrients, soak the wheat in lime water in advance until there is no white core, sterilize the mixture and then mix it with other raw materials.

[0082] The preparation methods of the tilapia peptide powder, ginseng peptide powder, wolfberry peptide powder and dendrobium officinale peptide are the same, and the preparation raw materials are tilapia, ginseng, wolfberry and dendrobium officinale respectively.

[0083] Taking the old man's head peptide powder as an example, its preparation method includes the following steps:

[0084] A1. Wash the old man's head mushroom, slice it, dry it to a water content of 10%, crush it, put it into a fermentation tank, add water to adjust the water content to 50% to obtain a fermentation substrate, add a fermentation agent, ferment at 42°C until the pH is 4, take it out, sterilize it, dehydrate and concentrate it to obtain a fermentation product;

[0085] A2, the fermented product was extracted twice with 70% ethanol solution under reflux, each time for 2 hours, filtered, and the two extracts were combined to obtain the old man head mushroom extract;

[0086] A3. Adjust the pH of the extract of Agaricus chinensis to 9, add alkaline protease, react at 55°C for 2 hours and then boil to inactivate, adjust the pH of the system to 7.5, add trypsin, react at 38°C for 2 hours and then boil to inactivate, adjust the pH of the system to 6.5, add papain, react at 52°C for 3 hours and then boil to inactivate, adjust the pH of the system to 2, add pepsin, react at 38°C for 3 hours and then boil to inactivate, cool to room temperature, centrifuge to obtain the supernatant, pass through an ultrafiltration membrane, concentrate and spray-dry to obtain.

[0087] The fermentation agent is Candida lipolytica, Bacillus subtilis and Lactobacillus acidophilus.

[0088] The dosage of the Candida lipolytica is 10 9 CFU / g fermentation substrate; the amount of Bacillus subtilis is 10 7 CFU / g fermentation substrate; the amount of Lactobacillus acidophilus is 10 7 CFU / g fermentation substrate.

[0089] The addition amount of the alkaline protease is 80U / g of Glechoma longituba, the addition amount of the trypsin is 800U / g of Glechoma longituba, the addition amount of the papain is 1200U / g of Glechoma longituba, and the addition amount of the pepsin is 800U / g of Glechoma longituba.

[0090] The preparation methods of the black sesame peptide powder, soybean peptide and black soybean peptide powder are the same, and the preparation raw materials are black sesame meal, soybean meal and black soybean meal respectively.

[0091] Taking black sesame peptide powder as an example, its preparation method includes the following steps: mixing black sesame meal and glucose, adding water to adjust the water content to 65%, adjusting the pH to 6, sterilizing and cooling to obtain a culture medium; inoculating mold into the culture medium, fermenting at 30°C for 4 days, passing through an ultrafiltration membrane and freeze-drying to obtain black sesame peptide powder.

[0092] The added amount of glucose is 8% of the mass of the black sesame meal.

[0093] The inoculation amount of the mold was 10%.

[0094] The molds are Aspergillus niger, Aspergillus oryzae and Monascus purpurogenus, and the mass ratio is 1:1:1.

[0095] The preparation process of the solid beverage for enhancing immunity and resisting fatigue comprises the following steps: mixing the raw materials uniformly and then sealing and packaging the mixture.

[0096] The molecular weight cut-off of the ultrafiltration membrane is 1 kDa lton.

[0097] Example 2

[0098] The difference between this embodiment and embodiment 1 is that the raw materials for preparing the immunity enhancing and anti-fatigue solid beverage include, by weight, 60 parts of morel mycelium powder, 7 parts of tilapia peptide powder, 7 parts of ginseng peptide powder, 7 parts of black sesame peptide powder, 7 parts of wolfberry peptide powder, 7 parts of dendrobium officinale peptide, 7 parts of soybean peptide, and 7 parts of black soybean peptide powder.

[0099] Comparative Example 1

[0100] The difference between this comparative example and Example 1 is that the raw materials for preparing the immunity enhancing and anti-fatigue solid beverage are, by weight, 48 parts of morel mycelium powder, 6 parts of tilapia peptide powder, 6 parts of ginseng peptide powder, 6 parts of black sesame peptide powder, 6 parts of wolfberry peptide powder, 6 parts of dendrobium officinale peptide, 6 parts of soybean peptide, and 6 parts of black soybean peptide powder.

[0101] Comparative Example 2

[0102] The difference between this comparative example and Example 1 is that the raw materials for preparing the immunity enhancing and anti-fatigue solid beverage include, by weight, 58 parts of morel mycelium powder, 6 parts of tilapia peptide powder, 6 parts of ginseng peptide powder, 6 parts of black sesame peptide powder, 6 parts of wolfberry peptide powder, 6 parts of dendrobium officinale peptide, and 6 parts of soybean peptide.

[0103] Comparative Example 3

[0104] The difference between this comparative example and Example 1 is that the preparation method of the Morchella mycelium powder comprises the following steps:

[0105] S1. Preparation of stock: inoculate the mother strain of Morchella into the culture medium and culture it at 25°C for 50 days to obtain the stock;

[0106] S2. Cultivated seed preparation: the original seed is transferred into a sterilized original seed nutrient bag and cultured at 25° C. for 30 days to obtain cultivated seeds;

[0107] S3, cultivation: the cultivation seeds are placed in sterilized cultivation seed nutrient bags and cultured at 25°C for 30 days, the seeds are sown and planted, and the temperature in the greenhouse is controlled at 25°C. After culturing for 60 days, morel mycelium is obtained;

[0108] S4, collecting Morchella mycelia, drying, dehydrating, and grinding to obtain Morchella mycelia powder.

[0109] Comparative Example 4

[0110] The difference between this comparative example and Example 1 is that, by weight, the nutrient formulas in the original seed nutrient bag, the cultivated seed nutrient bag and the foreign aid nutrient bag are consistent, namely, 55 parts of wheat, 18 parts of wheat bran, 8 parts of sawdust, 4 parts of rice husks and 1 part of lime.

[0111] Comparative Example 5

[0112] The difference between this comparative example and Example 1 is that the fermentation agent is Candida lipolytica, and the dosage is 10 9 CFU / g fermentation substrate.

[0113] Comparative Example 6

[0114] The difference between this comparative example and Example 1 is that the preparation method of the tilapia peptide powder comprises the following steps:

[0115] A1. Wash the old man's head mushroom, slice it, dry it to a water content of 10%, crush it, put it into a fermentation tank, add water to adjust the water content to 50% to obtain a fermentation substrate, add a fermentation agent, ferment at 42°C until the pH is 4, take it out, dehydrate it and concentrate it to obtain a fermentation product;

[0116] A2, the fermented product was extracted twice with 70% ethanol solution under reflux, each time for 2 hours, filtered, and the two extracts were combined to obtain the old man head mushroom extract;

[0117] A3. Adjust the pH of the extract of Agaricus chinensis to 9, add alkaline protease, react at 55°C for 2 hours, and then boil and inactivate. Adjust the pH of the system to 7.5, add trypsin, react at 38°C for 2 hours, and then boil and inactivate. Adjust the pH of the system to 6.5, add papain, react at 52°C for 3 hours, and then boil and inactivate. Cool to room temperature, centrifuge, take the supernatant, pass through an ultrafiltration membrane, and concentrate and spray-dry to obtain the product.

[0118] Comparative Example 7

[0119] The difference between this comparative example and Example 1 is that the mold is Aspergillus niger, and the inoculation amount is 10%.

[0120] Performance Testing

[0121] Select male 7-8 week old C57BL / 6 mice for animal experiment, mouse weight is controlled at 20-25g, 10 mice per sample, give common feed and low-dose solid beverage (0.5g / kg body weight / day) every day, continue feeding for 8 weeks, test the changes of gastrointestinal function, renal function and immune function of mice before and after feeding. Wherein the gastrointestinal function changes by testing the content change rate of beneficial bacteria (total amount of Bacteroides, Clostridium, Bifidobacterium, Lactobacillus) in the intestinal tract before and after feeding, the renal function changes by testing the uric acid content change rate in the blood before and after feeding, and the immune function changes by testing the IgG, IgA, and IgM change rates in the blood before and after feeding. Change rate = (pre-test data-post-test data) / pre-test data × 100%. All data are averaged. The results are shown in Table 1.

[0122] Table 1 Measurement results

[0123]

[0124] According to statistics, the solid beverages for enhancing immunity and resisting fatigue prepared by Examples 1 to 2 of the present invention have higher gastrointestinal function, kidney function, and immune function, indicating that taking the solid beverage can effectively regulate gastrointestinal function, enhance kidney health, and improve immunity. In Comparative Example 1, the amount of morel mycelium powder added is too little, in Comparative Example 2, black bean peptide powder is not added, in Comparative Example 3, no foreign aid nutrition bag is added, in Comparative Example 4, the formula of the foreign aid nutrition bag is consistent with the original seed nutrition bag, in Comparative Example 5, Bacillus subtilis and Lactobacillus acidophilus are not added as fermentation agents, in Comparative Example 6, pepsin is not used for hydrolysis, in Comparative Example 7, Aspergillus oryzae and Monascus purpurogenum are not added, and the prepared solid beverages have poor ability to regulate gastrointestinal function, kidney function, and immune function. Therefore, the solid beverages for enhancing immunity and resisting fatigue prepared by the raw materials and methods described in this application can effectively regulate gastrointestinal function, enhance kidney health, and enhance human immunity.

[0125] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A solid beverage for enhancing immunity and resisting fatigue, characterized in that: By weight, the preparation raw materials include 56-60 parts of morel mycelium powder, 5-7 parts of old man's head mushroom peptide powder, 5-7 parts of ginseng peptide powder, 5-7 parts of black sesame peptide powder, 5-7 parts of wolfberry peptide powder, 5-7 parts of dendrobium officinale peptide, 5-7 parts of soybean peptide, and 5-7 parts of black bean peptide powder.

2. The solid beverage for enhancing immunity and resisting fatigue according to claim 1, characterized in that: The preparation method of the morel mycelium powder comprises the following steps: S1. Preparation of stock: inoculate the mother stock of Morchella into the culture medium and culture it at 23-25°C for 45-60 days to obtain the stock; S2. Cultivated seed production: the original seed is transferred into a sterilized original seed nutrient bag and cultured at 23-25°C for 25-30 days to obtain cultivated seeds; S3, cultivation: the cultivation seeds are placed in sterilized cultivation seed nutrient bags and cultured at 23-25° C. for 25-30 days, the seeds are sown and planted, and the temperature in the greenhouse is controlled at 23-25° C. After culturing for 25-30 days, an external aid nutrient bag is added, and the culture is continued for 25-30 days, and the external aid nutrient bag is removed to obtain Morchella mycelium; S4, collecting Morchella mycelia, drying, dehydrating, and grinding to obtain Morchella mycelia powder.

3. The solid beverage for enhancing immunity and resisting fatigue according to claim 2, characterized in that: The nutrient formulas in the original seed nutrient bag and the cultivated seed nutrient bag are consistent by weight, including 50-60 parts of wheat, 15-20 parts of wheat bran, 5-10 parts of sawdust, 3-5 parts of rice husks, and 0.5-1 part of lime.

4. The solid beverage for enhancing immunity and resisting fatigue according to claim 3, characterized in that: Calculated by weight, the nutrients in the foreign aid nutrient bag include 50-60 parts of wheat, 15-20 parts of wheat bran, 10-15 parts of corn flour, 5-10 parts of soybean meal, and 0.5-1 part of lime.

5. The solid beverage for enhancing immunity and resisting fatigue according to claim 4, characterized in that: The preparation methods of the Laorentou fungus peptide powder, ginseng peptide powder, wolfberry peptide powder, and Dendrobium officinale peptide are the same, and the preparation raw materials are Laorentou fungus, ginseng, wolfberry, and Dendrobium officinale respectively; taking Laorentou fungus peptide powder as an example, its preparation method comprises the following steps: A1. Wash the old man's head mushroom, slice it, dry it until the water content is less than 10%, crush it and put it into a fermentation tank, add water to adjust the water content to 45% to 55% to obtain a fermentation substrate, add a fermentation agent, ferment at 40 to 45° C. until the pH is 4 to 4.5, take it out, dehydrate it and concentrate it to obtain a fermentation product; A2, extracting the fermented product with 65% to 75% ethanol solution twice under reflux, each time for 1 to 2 hours, filtering, combining the two extracts to obtain a Psoralea corylifolia extract; A3. Adjust the pH of the extract of Agaricus chinensis to 8-9, add alkaline protease, react at 50-60°C for 1-3 hours, and then boil and inactivate. Adjust the pH of the system to 7-8, add trypsin, react at 37-40°C for 1-3 hours, and then boil and inactivate. Adjust the pH of the system to 6-7, add papain, react at 50-55°C for 2-4 hours, and then boil and inactivate. Adjust the pH of the system to 2-3, add pepsin, react at 37-40°C for 2-4 hours, and then boil and inactivate. Cool to room temperature, centrifuge, take the supernatant, pass through an ultrafiltration membrane, and concentrate and spray-dry to obtain.

6. The solid beverage for enhancing immunity and resisting fatigue according to claim 5, characterized in that: The fermentation agent includes Candida lipolytica, Bacillus subtilis and Lactobacillus acidophilus.

7. The solid beverage for enhancing immunity and resisting fatigue according to claim 6, characterized in that: The dosage of the Candida lipolytica is 10 8 -10 9 CFU / g fermentation substrate; the amount of Bacillus subtilis is 10 7 -10 8 CFU / g fermentation substrate; the amount of Lactobacillus acidophilus is 10 6 ~10 7 CFU / g fermentation substrate.

8. The solid beverage for enhancing immunity and resisting fatigue according to claim 1, characterized in that: The preparation methods of the black sesame peptide powder, soybean peptide and black soybean peptide powder are consistent, and the preparation raw materials are black sesame meal, soybean meal and black soybean meal respectively; taking black sesame peptide powder as an example, its preparation method comprises the following steps: mixing black sesame meal and glucose, adding water to adjust the water content to 60%-70%, adjusting the pH to 5.5-6.5, sterilizing and cooling to obtain a culture medium; inoculating mold into the culture medium, fermenting at 25-35° C. for 3-5 days, passing through an ultrafiltration membrane and freeze-drying to obtain black sesame peptide powder.

9. The solid beverage for enhancing immunity and resisting fatigue according to claim 8, characterized in that: The molds include Aspergillus niger, Aspergillus oryzae and Monascus purpurogenum.

10. A process for preparing the solid beverage for enhancing immunity and resisting fatigue according to any one of claims 1 to 9, characterized in that: include: Mix the raw materials evenly and seal them.

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