Fracturable solid-phase alkylation material and preparation and application thereof

Through the use of acid-breakable solid-phase alkylated materials, the problems of low recovery rate and poor anti-interference ability in clinical proteomic sample preparation are solved, and high-throughput, strong anti-interference protein sample preparation is achieved, which expands detection coverage and supports a variety of research methods.

CN119708512AActive Publication Date: 2025-03-28DALIAN INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES
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Patent Information

Application Number
CN202311265720.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-09-28
Publication Date
2025-03-28
Estimated Expiration
2043-09-28

AI Technical Summary

Technical Problem

The prior art has low recovery, poor anti-matrix interference ability and low flux in clinical proteome sample preparation, resulting in low analysis coverage.

Method used

Acid-breakable solid-phase alkylated materials are used to modify epoxy microspheres through covalent bonding, increasing the characteristics of bilateral acid-breakable amino link arms to achieve high recovery capture and rapid separation of proteins.

Benefits of technology

It significantly improves the preparation flux and anti-interference ability of proteomic samples, increases the enrichment of thiol-containing peptides, expands the coverage of detection proteins, and supports the TOP-DOWN and BOTTOM-UP research methods.

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Abstract

The invention relates to a breakable solid-phase alkylation material as well as preparation and application thereof, epoxy microspheres are taken as a carrier, polyethyleneimine, an acid breakable connecting arm and iodoacetic acid-N-succinamide ester are sequentially modified in a covalent bonding manner, and the breakable solid-phase alkylation material is prepared. The material selectively reacts with sulfydryl on protein, efficient enrichment and treatment of protein in complex biological samples such as cells, tissues and body fluid can be achieved, lossless release of protein and peptide fragments can be achieved by breaking connecting arms under the acidic condition, and important technical support is expected to be provided for proteome deep coverage analysis and protein variant analysis.
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