Enzymolysis preparation process of albumin oligopeptide

Through the method of combining structural modification with multiple enzymatic system and multi-stage membrane separation system, the problems of low diversity, poor stability and single action mechanism in the existing albumin oligomeric peptide preparation technology are solved, and efficient, multifunctional and high-performance albumin oligomeric peptide preparation is achieved, with wide clinical application prospects.

CN119709803APending Publication Date: 2025-03-28HUBEI NUTRATIDE BIOTECH CO LTD
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Patent Information

Application Number
CN202411068287.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-08-06
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The existing albumin oligopeptide preparation technology has problems such as low peptide sequence diversity, difficulty in controlling the degree of hydrolysis, insufficient product purity and safety, low bioavailability, poor stability, a single target mechanism, lack of tissue specificity and high cost, which limits its clinical application and marketing promotion.

Method used

The recombinant albumin is enzymatically purified and purified by using a multiple enzymatic system and a multi-stage membrane separation system, combining N-terminal cysteine ​​modification and C-terminal glycyl-prolyl modification to optimize the structure and function of the peptide, and ensuring the high immune activity of the product through a variety of immune activity evaluation methods.

Benefits of technology

It has achieved efficient and accurate preparation technology, improved the bioavailability and stability of the product, and has multi-target immunomodulation, epithelial barrier protection and broad-spectrum anti-inflammatory effects, reduced toxicity and side effects, and has good industrialization potential.

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Abstract

The invention relates to the technical field of albumin oligopeptide enzymolysis, in particular to an albumin oligopeptide enzymolysis preparation process which comprises the following steps: (1) constructing and expressing recombinant albumin containing a KRPAGY repetitive sequence; (2) purifying the recombinant albumin; (3) carrying out enzymolysis on the purified recombinant albumin by using a multiple enzymolysis system; (4) purifying the enzymolysis product through a multi-stage membrane separation system; (5) carrying out structural modification on the purified peptide fragment; and (6) evaluating the immunocompetence of the modified peptide fragment, through the design of a multiple enzymolysis system, accurate hydrolysis of albumin is realized, and the yield and specificity of the target peptide fragment are remarkably improved. The innovative membrane separation technology greatly improves the separation and purification efficiency; through N-terminal cysteine modification and C-terminal glycyl-prolyl modification, the stability and membrane permeability of the peptide fragment in the gastrointestinal tract environment are significantly improved.
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