Porphyra haitanensis conchospore seedling collecting device and seedling collecting method

Abstract

The invention relates to the technical field of artificial culture of porphyra haitanensis, in particular to a porphyra haitanensis conchospore seedling collecting device and a seedling collecting method using the same. The seedling collecting device comprises a nursery pond, an inflating device, a supporting rod and a net bag; the inflation device is arranged at the bottom of the nursery pond, a first air outlet is formed in the top of the inflation device, and the inflation device is sequentially connected with a gas flow valve and an air pump outside the nursery pond; the supporting rod is erected at the top of the nursery pond, a net bag is hung on the supporting rod, shells attached with shell conchocelis are arranged in the net bag, the size of meshes of the net bag is smaller than that of the shells, and the color, close to a net opening, of the net bag is white; and the forming position of the first air outlet of the inflating device corresponds to the hanging position of the mesh bag.

Description

Technical Field

[0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a device and a method for collecting seedlings of conchospores of Porphyra haitanensis. Background Art

[0002] Porphyra haitanensis is one of the most commercially valuable species in the genus Porphyra and is widely cultivated in coastal areas south of the Yangtze River in my country. The life cycle of Porphyra haitanensis consists of a microscopic shell filament stage (sporophyte) and a macroscopic thallus stage (gametophyte). The mature thallus releases fruit spores, which attach to the calcareous shells and continue to germinate and grow into shell filaments; the shell filaments further develop into sporangial branches, which release conchospores when mature. Conchospores attach to suitable substrates such as net curtains and eventually develop into edible thallus after cultivation management.

[0003] The release pattern of conchospores is not fixed. Some conchospores have an obvious release peak in a specific time period, such as a concentrated release at around 10 a.m., while other conchospores have no obvious release peak and may be released at any time from 7 a.m. to 1 p.m. After the released conchospores attach to a suitable substrate (such as a net curtain), they gradually grow into seedlings through subsequent cultivation management, and eventually develop into harvestable fronds.

[0004] Since conchospores will sink under the influence of gravity, if they are deposited too much at the bottom of the water body and stay too long, it will lead to poor water exchange, thus affecting their vitality. This situation will significantly reduce the number and quality of conchospores attached to the net curtain, and have an adverse effect on the yield and quality of the thallus of Porphyra. Therefore, the collection of conchospore seedlings has become a key link in the production of Porphyra, and is also one of the important factors determining the yield and quality.

[0005] Since the artificial propagation of Porphyra haitanensis began to show success in the 1960s, people have gradually summarized a set of technical measures covering all aspects of production. In the traditional seedling collection process, since the gravity of Porphyra conchospores is greater than their buoyancy, they are easy to settle naturally. Therefore, the traditional method of collecting Porphyra conchospores is to place the shells with mature filaments on the top of the net curtain in a seedling pool filled with seawater ( Figure 2 ). When the conchospores are released by the conchospores, the conchospores are evenly distributed in the water body for seedling collection by artificial water splashing, thus completing the indoor dyeing cloth seedling collection. With the advancement of technology, artificial water splashing has gradually been replaced by water pump flushing ( Figure 3 ), which improves the operation efficiency. Based on the traditional indoor dyeing cloth seedling collection technology, the waterwheel seedling collection technology has been developed ( Figure 4), this method uses a large roller to wrap the net curtain, and the motor drives the roller to rotate. The roller is placed on a pool of water with shell filaments below. The shell spores released by the filaments float in the water, and the net curtain immersed in the water absorbs the shell spores. When the shell spore density on the seedling curtain is confirmed to meet the requirements, the net curtain is dissociated and the seedling collection is completed. In order to reduce the amount of manual operation of shell filaments during transportation, a seedling collection method based on the cultivation sea area or dock has also been developed. This method uses the sea area laver operation boat, lays impermeable plastic cloth or tarpaulin in the boat, and directly carries out dyeing cloth type seedling collection ( Figure 5 However, due to the difficulty in conducting microscopic inspections during the sea area seedling collection process, accurate seedling collection cannot be achieved, which often leads to excessive attachment of conchospores and waste of seedlings.

[0006] However, the above-mentioned traditional and optimized methods for collecting seedlings of Porphyra haitanensis still have many shortcomings, which are mainly related to the uncertainty of the release time and release amount of shell spores and their vertical sinking characteristics. Since the shell spores are basically in a vertical sinking state after release, artificial watering or water pump flushing cannot timely and effectively distribute the shell spores evenly in the water body, and it is difficult to meet the requirements of uniform attachment to the net. At the same time, the shell spores are not accurately counted before attaching to the net, and accurate seedling attachment cannot be achieved, resulting in difficulty in controlling the attachment density, which affects the subsequent cultivation effect. The water quality may be unstable due to heat during the water pump flushing process, and the drainage is usually concentrated in one direction. The water inlet is often fixed during operation, and it is difficult to ensure uniform flow of the entire water body. This uneven flow often leads to excessive deposition of shell spores at the bottom or in local areas, and the residence time is too long, thereby affecting the vitality of the shell spores. In addition, foreign matter pollution caused by manual entry into the seedling collection pool may also cause water quality changes. At the same time, shell spores are easily attached to objects such as pants and shoes of staff, causing the loss of shell spores, further affecting the number and quality of shell spores attached to the net curtain. These problems not only limit the efficient collection of conchospores, but also have an adverse impact on the yield and quality of the thallus of Porphyra haitanensis, becoming an important bottleneck in the development of the industry. Summary of the invention

[0007] In view of the above-mentioned shortcomings of the prior art, the present invention provides a device and a method for collecting seedlings of conchospores of Porphyra haitanensis, thereby making up for the shortcomings of the prior art.

[0008] The present invention first provides a device for collecting seedlings of Porphyra haitanensis conchospores, which comprises a seedling raising pool, an aeration device, a support rod and a net bag; The inflation device is arranged at the bottom of the nursery pool, and a first air outlet is opened at the top thereof. The inflation device is connected to a gas flow valve and an air pump in sequence outside the nursery pool; The support rod is set up on the top of the nursery pond, and a net bag is hung on the support rod. The net bag contains shells with shell filaments attached. The size of the mesh of the net bag is smaller than the size of the shells, and the color of the net bag near the net mouth is white; The opening position of the first air outlet of the inflatable device corresponds to the hanging position of the net bag.

[0009] Preferably, the inflation device is an inflation pipe, and the inflation pipe is arranged in a serpentine shape at the bottom of the nursery pond.

[0010] Furthermore, second air outlets are respectively provided on both side surfaces of the inflation pipe.

[0011] The present invention also provides a method for collecting seedlings of conchospores of Porphyra haitanensis, wherein the method comprises collecting seedlings using the above-mentioned seedling collecting device; More specifically, the seedling collecting method comprises the following steps: S1: adding appropriate seawater to the nursery pond, starting the air pump, adjusting the gas flow valve, and ensuring that the gas from the first air outlet can reach the surface of the water body; placing shells with shell filaments in each mesh bag, hanging the mesh bags on the support rods at intervals, and setting up the support rods with the mesh bags hung on the top of the nursery pond, so that the shells in the mesh bags are completely immersed in the seawater, the hanging position of the mesh bags corresponds to the position of the first air outlet of the inflation device, and the white part of the mesh bags is fully or partially exposed to the water surface; S2 When a large amount of purple-red conchospores are observed to accumulate in the white part of the net bag, the peak of conchospore release is determined; S3 then counted the number of conchospores in the water column; S4: when it is determined that the number of conchospores in the water body reaches the expected value after counting in step S3, the net bag is lifted, or the net bag is placed on one side, and the net curtain is immersed in seawater to collect seedlings.

[0012] Furthermore, in step S1, the inflation can reach the surface of the water body, which means that by controlling the gas flow rate and flow rate, small bubbles can appear on the water surface, making the water surface fluctuate by about 1 cm. It is necessary to frequently check the size and uniformity of the bubbles and adjust the gas volume in time.

[0013] Furthermore, Alteromonas ( Alteromonas sp. MS) strain, the Alteromonas ( Alteromonas sp. MS) strain was deposited in the China Type Culture Collection, Wuhan University, Wuhan, China on November 21, 2024, with the deposit number CCTCC NO: M 20242609.

[0014] The beneficial effects of the present invention are as follows: First, it effectively improves the uniform distribution of shell spores in the seedling collection pool, reduces the probability of shell spores depositing at the bottom, and combines timely counting of shell spores after the peak of release to achieve uniform attachment to the net and precise attachment to seedlings.

[0015] Second, an aerating device is used instead of a water pump to flush water. On the one hand, vertical water circulation is achieved to reduce the probability of shell spores settling at the bottom, overcoming the heat generated by the water pump and the single horizontal flow. On the other hand, sufficient oxygen is provided, so that the shell spores germinate well and have strong vitality, which helps the shell spores to firmly adhere to the net, greatly increasing the release quality and germination effect of the shell spores.

[0016] Third, by setting up mesh bags and support structures, operators are prevented from entering the seedling collection pool to affect water quality and conchospore loss.

[0017] Fourth, Alteromonas ( Alteromonas sp. MS) has an antibacterial mechanism against the halophilic bacteria that are the pathogens of Porphyra filamentosa yellow spot disease. Alteromonas may inhibit the growth of pathogenic halophilic bacteria by secreting antibiotics, enzymes or other metabolites.

[0018] Fifth, Alteromonas ( Alteromonas sp. MS) can promote the maturation of conchospores, promote centralized harvesting when picking laver seedlings, improve work efficiency, and minimize the possibility of immature or aborted conchospores. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural diagram of the seedling collection device; Figure 2 It is a dyeing cloth seedling collection artificial flushing mode; Figure 3 It is the flushing mode of the dyeing seedling water pump; Figure 4 It is a waterwheel-type seedling collection mode; Figure 5 It is a mode of collecting seedlings by using sea-area laver operation boats; Figure 6 It is the antibacterial effect of the tested strain on pathogenic bacteria and halotrophic bacteria. DETAILED DESCRIPTION

[0020] The present invention will be further described below in conjunction with the accompanying drawings and embodiments: Example 1 Figure 1 As shown, a device for collecting seedlings of Porphyra haitanensis conchospores comprises a seedling raising pool 1, an aeration device, 3 support rods and a mesh bag 4. The seedling raising pool 1 is 10×4×1 m (length×width×depth), and is filled with seawater 6, the depth of which is 0.6 m.

[0021] The aeration device uses an aeration pipe 2, which is arranged at the bottom of the nursery pool in a serpentine shape along the long side of the pool, with an interval of 80 cm between adjacent aeration pipes, and a first air outlet 2-1 is arranged at intervals of 80 cm at the top of the aeration pipe 2 along the short side of the pool. The aeration pipe 2 is connected to a gas flow valve 8 and an air pump 7 in sequence outside the nursery pool. The aeration device can also use an air stone, and an air stone is arranged at intervals of 80 cm at the bottom of the nursery pool 1.

[0022] The support pole 3 is preferably a bamboo pole with a diameter of about 10 cm, a length of 4 meters, and a number of 12. A mesh bag 4 is hung on each bamboo pole at intervals of 80 cm. The mesh bag is 1 m long and 60 cm in diameter. Shells 5 cultivated with shell filaments are placed in each mesh bag 4. The size of the mesh hole of the mesh bag 4 is smaller than the size of the shell 5. The bamboo poles with the mesh bags 4 hung are arranged at intervals of 80 cm on the top of the nursery pond 1 along the long side direction of the nursery pond 1. The shells 5 in the mesh bag are completely immersed in the seawater 6, and the hanging position of the mesh bag 4 corresponds to the opening position of the first air outlet 2-1 of the inflatable device. The mesh bag 4 is made of polyethylene material, and the color of the mesh bag 4 body near the water surface is white. The seedling pool 1 can accommodate 120 mu of mesh bags 4 of shell filaments.

[0023] Example 2 The aeration device adopts an aeration pipe 2, which is arranged at the bottom of the nursery pool 1 in a serpentine shape extending along the long side of the pool, with an interval of 80 cm between adjacent aeration pipes, and a first air outlet 2-1 is arranged at every 80 cm on the top of the pipe along the short side of the pool, and second air outlets 2-2 are arranged on both sides of the aeration pipe between two adjacent first air outlets 2-1. The aeration pipe 2 is connected to a gas flow valve 8 and an air pump 7 in sequence outside the nursery pool. The arrangement of the first air outlet 2-1 and the second air outlet 2-2 can form a circulating airflow in the water body, which can stimulate the formation of sporangium branches on the one hand, and reduce the deposition of conchospores after the conchospores are released on the other hand.

[0024] Example 3 A method for collecting seedlings of conchospores of Porphyra haitanensis, using the seedling collecting device in Example 1 or Example 2, comprises the following steps: S1 adds seawater 6 to the nursery pond 1 with a water depth of 0.6m, starts the air pump 7, adjusts the gas flow valve 8 to ensure that the gas from the first air outlet 2-1 can reach the surface of the water body, places shells 5 with shell filaments in each mesh bag 4, hangs the mesh bags 4 on the support rods 3 at intervals of 0.8m, and arranges the support rods 3 with the mesh bags hung on them at intervals of 0.8m along the length of the pond on the top of the nursery pond 1, so that the shells in the mesh bags are completely immersed in the seawater 6, and the white part of the mesh bags is fully or partially exposed to the water surface, and the hanging position of the mesh bags 4 corresponds to the position of the first air outlet 2-1 on the top of the inflation device; S2 When a large amount of purple-red conchospores are observed to accumulate in the white part of the net bag, the peak of conchospore release is determined; S3 then counted the number of conchospores in the water column; S4, when it is determined that the number of conchospores in the water body reaches the expected value after counting in step S3, the net bag 4 is lifted by lifting the support rod, or the net bag 4 is placed on one side, and the net curtain is immersed in the sea water 6 to collect seedlings according to the requirement of 200 to 500 million conchospores / mu; S5 re-places the net bag 4 containing the shells 5 above the net curtain, and the shell spores that continue to be released by the shell filaments can continue to adhere to the net curtain.

[0025] In step S1, the inflation can reach the surface of the water body, which means that the gas flow rate and flow rate are controlled to meet the requirements of small bubbles appearing on the liquid surface, so that the liquid surface fluctuates by about 1 cm, and the size and uniformity of the bubbles need to be checked frequently and adjusted in time.

[0026] Each mesh bag 4 is placed with 350 pairs of scallop shells cultured with shell filaments or 1500 clam shells cultured with shell filaments.

[0027] Example 4: Inhibition zone experiment S1 strain culture: The Alteromonas strain ( Alteromonas sp. MS) was activated and inoculated into 2216E liquid culture medium, cultured at 25°C with shaking for 24 h, cultured at 150 rpm until the OD value was 0.19, and the supernatant (containing metabolites) was collected by centrifugation.

[0028] Among them, Alteromonas Alteromonas sp. MS) strain was screened by the inventor from Porphyra and deposited in the China Type Culture Collection of Wuhan University, Wuhan, China on November 21, 2024, with the deposit number CCTCCNO: M 20242609.

[0029] The causative bacteria of yellow spot disease of Porphyra filamentosa and the haloth bacteria were activated and inoculated into 2216E liquid culture medium and cultured at 25°C with shaking for 24 hours until the logarithmic growth phase.

[0030] S2 Spread the salt-tolerant bacteria evenly on the agar plate and soak the sterile filter paper in the medium containing the bacteria ( Alteromonas sp. MS) supernatant, and placed on agar plates in triplicate. A filter paper disc soaked in sterile culture medium was used as the control group.

[0031] S3 was cultured at 25°C for 24-48 h, and the formation of inhibition zones was observed and their diameters were measured.

[0032] The experimental results are as follows Figure 6As shown: obvious antibacterial zones were formed around filter paper pieces 1-3 in the three experimental groups, with an average diameter of 11.5 mm. No antibacterial zone was formed in the control group 4.

[0033] Example 5: Co-culture experiment S1 strain culture: same as Example 4.

[0034] S2: Mix Alteromonas and halophilic bacteria in a 1:1 ratio and inoculate them into 2216E liquid culture medium.

[0035] S3 set up a control group: only inoculated with halothiocyanate bacteria.

[0036] S4 was cultured at 25 °C for 48 h, samples were taken regularly, and the growth of pathogenic bacteria (OD600 value) was determined by spectrophotometer.

[0037] The experimental results are shown in Table 1: The growth of pathogenic bacteria in the experimental group was significantly inhibited, and the OD600 value was reduced by about 65% compared with the control group. The halotrophic bacteria in the control group grew normally, and the OD600 value increased over time.

[0038] Table 1: Growth of halophilic bacteria (OD600 value)

[0039] Through the above inhibition zone experiment and co-culture experiment, it can be seen that Alteromonas ( Alteromonas sp. MS) has an antibacterial mechanism against the pathogenic halobacteria of yellow spot disease. Alteromonas may inhibit the growth of pathogenic halobacteria by secreting antibiotics, enzymes or other metabolites.

[0040] Example 6 A method for collecting seedlings of conchospores of Porphyra haitanensis, using the seedling collecting device in Example 1 or Example 2, comprises the following steps: Preparation of S1 bacterial preparation The Alteromonas strain ( Alteromonas sp. MS) was activated and 2216E medium was used. The inoculation density was 2.5 μl of bacterial suspension was added to 35 ml of medium. The temperature was 25°C and the culture speed was 150 rpm until the OD value was 0.19. The supernatant was collected by centrifugation.

[0041] Alteromonas Alteromonas sp. MS) strain was deposited in the China Type Culture Collection, Wuhan University, Wuhan, China on November 21, 2024, with the deposit number CCTCC NO: M 20242609.

[0042] S2: adding seawater 6 into the nursery pond 1, the water depth is 0.6m, adding the bacterial preparation prepared in step S1 into the seawater 6, the concentration of the bacterial preparation is 1:1000.

[0043] S3 starts the air pump 7 and adjusts the gas flow valve 8 to ensure that the air from the first air outlet 2-1 can reach the water surface. Shells 5 with shell filaments are placed in each mesh bag 4. The mesh bags 4 are hung on the support rods 3 at intervals of 0.8 m in sequence. The support rods 3 with the mesh bags hung are arranged on the top of the nursery pond 1 at intervals of 0.8 m along the length of the pond, so that the shells in the mesh bags 4 are completely immersed in the sea water 6, and the white part of the mesh bags is fully or partially exposed above the water surface. The hanging position of the mesh bag 4 corresponds to the position of the first air outlet 2-1 on the top of the inflation device.

[0044] S4 When a large amount of purple-red conchospores accumulate in the white part of the net bag, the peak of conchospore release is determined.

[0045] S5 then counts the number of conchospores in the water column.

[0046] S6: When it is determined that the number of conchospores in the water body reaches the expected value after counting in step S3, the net bag 4 is lifted by lifting the support rod, or the net bag 4 is placed on one side, and the net curtain is immersed in the sea water to collect seedlings according to the requirement of 200 to 500 million conchospores / mu.

[0047] S7 re-places the net bag 4 containing shells above the net curtain, and the shell spores continuously released by the shell filaments can continue to adhere to the net curtain.

[0048] Example 7 A method for collecting seedlings of conchospores of Porphyra haitanensis, using the seedling collecting device in Example 1 or Example 2, comprises the following steps: Preparation of S1 bacterial preparation The Alteromonas strain ( Alteromonas sp. MS) was activated and 2216E medium was used. The inoculation density was 2.5 μl of bacterial suspension was added to 35 ml of medium. The temperature was 25°C and the culture speed was 150 rpm until the OD value was 0.19. The supernatant was collected by centrifugation.

[0049] S2: adding seawater 6 into the nursery pond 1, the water depth is 0.6m, and adding the bacterial preparation prepared in step S1 into the seawater 6, the concentration of the bacterial preparation is 1:2500.

[0050] S3 starts the air pump 7 and adjusts the gas flow valve 8 to ensure that the inflation of the first air outlet 2-1 can reach the water surface, places shells 5 with cultivated shell filaments in each mesh bag 4, and hangs the mesh bags 4 on the support rods 3 at intervals of 0.8m in sequence, and arranges the support rods 3 with the mesh bags hung on the top of the nursery pond 1 at intervals of 0.8m along the length of the pond, so that the shell filaments in the mesh bags are completely immersed in the seawater 6, and the white part of the mesh bags is fully or partially exposed above the water surface, and the hanging position of the mesh bag 4 corresponds to the position of the first air outlet 2-1 on the top of the inflation device.

[0051] S4 When a large amount of purple-red conchospores accumulate in the white part of the net bag, the peak of conchospore release is determined.

[0052] S5 then counts the number of conchospores in the water column.

[0053] S6: When it is determined that the number of conchospores in the water body reaches the expected value after counting in step S3, the net bag 4 is lifted by lifting the support rod, or the net bag 4 is placed on one side, and the net curtain is immersed in the sea water to collect seedlings according to the requirement of 200 to 500 million conchospores / mu.

[0054] Example 8 A method for collecting seedlings of conchospores of Porphyra haitanensis, using the seedling collecting device in Example 1 or Example 2, comprises the following steps: Preparation of S1 bacterial preparation The Alteromonas strain ( Alteromonas sp. MS) was activated and 2216E medium was used. The inoculation density was 2.5 μl of bacterial suspension was added to 35 ml of medium. The temperature was 25°C and the culture speed was 150 rpm until the OD value was 0.19. The supernatant was collected by centrifugation.

[0055] S2: adding seawater 6 into the nursery pond 1, the water depth is 0.6m, and adding the bacterial preparation prepared in step S1 into the seawater 6, the concentration of the bacterial preparation is 1:4000.

[0056] S3 starts the air pump 7 and adjusts the gas flow valve 8 to ensure that the inflation of the first air outlet 2-1 can reach the water surface, places shells 5 with cultivated shell filaments in each mesh bag 4, and hangs the mesh bags 4 on the support rods 3 at intervals of 0.8m in sequence, and arranges the support rods 3 with the mesh bags 4 hung at intervals of 0.8m along the length of the pool on the top of the nursery pool 1, so that the shell filaments in the mesh bags 4 are completely immersed in the seawater 6, and the white part of the mesh bags is fully or partially exposed above the water surface, and the hanging position of the mesh bag 4 corresponds to the position of the first air outlet 2-1 on the top of the inflation device.

[0057] S4 When a large amount of purple-red conchospores accumulate in the white part of the net bag, the peak of conchospore release is determined.

[0058] S5 then counts the number of conchospores in the water column.

[0059] S6: When it is determined that the number of conchospores in the water body reaches the expected value after counting in step S3, the net bag 4 is lifted by lifting the support rod, or the net bag 4 is placed on one side, and the net curtain is immersed in the sea water to collect seedlings according to the requirement of 200 to 500 million conchospores / mu.

[0060] The maturity rate of conchospores of Porphyra haitanensis and the frequency of harvesting on the same day in Examples 6, 7 and 8 were compared with those without adding bacterial solution.

[0061] Table 2: Comparison of the maturity rate and harvesting frequency of Porphyra conchospores without and with bacterial agents

[0062] It can be seen that Alteromonas ( Alteromonas sp. MS) can centrally promote the maturation of conchospores, collect them in a centralized manner, improve work efficiency, and minimize the possibility of immaturity or abortion of conchospores; on the other hand, it has an inhibitory effect on the pathogenic bacteria and halothiobacteria of yellow spot disease.

[0063] Technical features not described in the present invention can be achieved through or by adopting existing technologies and will not be described in detail here. Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. A device for collecting conchospores of Porphyra haitanensis, characterized in that: Includes nursery pond, aeration device, support poles and mesh bag; The inflation device is arranged at the bottom of the nursery pool, and a first air outlet is opened at the top thereof. The inflation device is connected to a gas flow valve and an air pump in sequence outside the nursery pool; The support rod is set up on the top of the nursery pond, and a net bag is hung on the support rod. The net bag contains shells with shell filaments attached. The size of the mesh of the net bag is smaller than the size of the shells, and the color of the net bag near the net mouth is white; The opening position of the first air outlet of the inflatable device corresponds to the hanging position of the net bag.

2. The device for collecting conchospores of Porphyra haitanensis according to claim 1, characterized in that: The inflation device is an inflation pipe, and the inflation pipe is arranged in a serpentine shape at the bottom of the nursery pond.

3. The device for collecting conchospores of Porphyra haitanensis according to claim 2, characterized in that: Second air outlets are respectively provided on both side surfaces of the inflation pipe.

4. The device for collecting conchospores of Porphyra haitanensis according to claim 1, characterized in that: The inflating device is an air stone.

5. The device for collecting conchospores of Porphyra haitanensis according to claim 1, characterized in that: The support rod is made of bamboo.

6. A method for collecting conchospores of Porphyra haitanensis, characterized in that: Using the seedling picking device according to any one of claims 1 to 5, comprising the following steps: S1: adding appropriate seawater to the nursery pond, starting the air pump, adjusting the gas flow valve, and ensuring that the gas from the first air outlet can reach the surface of the water body; placing shells with shell filaments in each mesh bag, hanging the mesh bags on the support rods at intervals, and setting up the support rods with the mesh bags hung on the top of the nursery pond, so that the shells in the mesh bags are completely immersed in the seawater, the hanging position of the mesh bags corresponds to the position of the first air outlet of the inflation device, and the white part of the mesh bags is fully or partially exposed to the water surface; S2 When a large amount of purple-red conchospores are observed to accumulate in the white part of the net bag, the peak of conchospore release is determined; S3 then counted the number of conchospores in the water column; S4: when it is determined that the number of conchospores in the water body reaches the expected value after counting in step S3, the net bag is lifted, or the net bag is placed on one side, and the net curtain is immersed in seawater to collect seedlings.

7. The method for collecting conchospores of Porphyra haitanensis according to claim 6, characterized in that: In step S1, the inflation can reach the surface of the water body, which means that the gas flow rate and flow rate are controlled to meet the requirements of small bubbles appearing on the water surface and making the water surface fluctuate by about 1 cm. It is necessary to frequently check the size and uniformity of the bubbles and adjust the gas volume in time.

8. The method for collecting conchospores of Porphyra haitanensis according to claim 6, characterized in that: In step S4, after the net curtain is immersed in seawater to collect seedlings, the net bag containing shells is placed on the net curtain again.

9. A method for collecting conchospores of Porphyra haitanensis seedlings according to any one of claims 6 to 8, characterized in that: Alteromonas sp. ( Alteromonas sp. MS) strain, the Alteromonas ( Alteromonas sp. MS) strain was deposited in the China Type Culture Collection, Wuhan University, Wuhan, China on November 21, 2024, with the deposit number CCTCC NO: M 20242609.

10. The method for collecting conchospores of Porphyra haitanensis according to claim 9, characterized in that: The bacterial preparation preparation method is to prepare the strain of Alteromonas ( Alteromonas sp. MS) was activated and 2216E medium was used. The inoculation density was 2.5 μl of bacterial suspension was added to 35 ml of medium. The temperature was 25°C and the culture speed was 150 rpm until the OD value was 0.

19. The supernatant was collected by centrifugation.

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