Preparation method of areca-nut core fermentation product and application of areca-nut core fermentation product in feed

Abstract

The preparation method specifically comprises the following steps: S1, adding saccharomyces cerevisiae and distilled water into a strain fermentation tank according to the ratio of 1: 5, then adding 1% of soybean molasses with the sugar content of 50%, and performing intermittent aerobic stirring culture at the constant temperature of 35-40 DEG C for 2-4 hours to obtain a bacterial solution; s2, crushing the wet betel nut cores, and uniformly adding 0.5% of the bacterial liquid prepared in the step S1 in the crushing process to obtain a crushed material to be fermented; and S3, adding the crushed material to be fermented in the step S2 into a fermentation tank or a fermentation bag, and performing anaerobic fermentation for 48 hours to obtain the wet betel nut core fermented material. The areca seed core fermented product is used as a feed raw material or an additive, and is subjected to microbial fermentation or generates a large amount of beneficial bacteria, enzymes and prebiotics, so that the areca seed core fermented product is used for preparing the fermented product to be applied to animal feed after being subjected to microbial fermentation.

Description

Technical Field

[0001] The invention relates to the technical field of fermented feed, and in particular to a preparation method of betel nut core fermented product and application thereof in feed. Background Art

[0002] Betel nut green fruit is produced in Hainan and is an important Chinese medicinal material, one of the four major southern medicines. In the field of edible betel nut production in my country, a large amount of by-product betel nut core is produced every year. Since betel nut core has high water content and is not easy to preserve, it will cause environmental pollution and waste of resources.

[0003] The applicant has found through research that discarded betel nut cores have extremely high recycling value. Specifically: 1. According to tests, betel nut core contains starch, polysaccharides, protein, arecoline, amino acids, etc., which can be used as feed raw materials in animal breeding, and it is in line with the national action plan to promote grain conservation in the breeding industry; research shows that betel nut core contains arecoline, which has very good physiological functions such as antibacterial and anti-inflammatory, insect repellent and insecticide, anti-oxidation, and providing immunity to animals; 2. The production of chewable betel nut requires high-temperature steaming and does not contain any bacteria. The water content of the betel nut core after steaming is generally around 35%, which is very consistent with the conditions of microbial solid fermentation substrate (according to existing fermentation technology, the raw materials used as fermentation substrates must be sterilized at high temperature before fermentation, and the water content of the raw materials used as substrates during solid fermentation must be controlled at around 40%). Therefore, the wet betel nut core can be fermented and applied to animal feed, which can realize the recycling of resources and comply with the national grain-saving action plan for the breeding industry, turning waste into treasure and improving economic benefits.

[0004] Therefore, based on the above analysis, it can be seen that how to recycle betel nut core is an urgent problem to be solved. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a method for preparing betel nut core fermentation product.

[0006] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solution: A method for preparing a betel nut core fermented product, specifically comprising the following steps: S1. Add brewer's yeast and distilled water in a ratio of 1:5 into a bacterial fermentation tank, then add 1% soybean molasses with a sugar content of 50%, and culture at a constant temperature of 35-40°C with intermittent aerobic stirring for 2-4 hours to obtain a bacterial solution; S2, crushing the wet betel nut core, and evenly adding 0.5% of the bacterial solution prepared in step S1 during the crushing process to obtain the crushed material to be fermented, the particle size of the crushed material to be fermented needs to be 80% through a 40-mesh sieve, 100% through a 20-mesh sieve, and the moisture content is 35-45%; S3. Add the crushed material to be fermented in step S2 into a fermentation tank or a fermentation bag, seal the fermentation tank or the fermentation bag, and carry out anaerobic fermentation for 48 hours at a constant temperature of 40-55° C. to obtain the wet betel nut core fermented material.

[0007] As a further preferred embodiment of the present invention, the water content of the wet betel nut core fermentation product is ≥35%.

[0008] As a further preferred embodiment of the present invention, the wet fermented betel nut core fermented material obtained in step S3 is dried to obtain dry betel nut core fermented material.

[0009] As a further preferred embodiment of the present invention, the water content of the fermented dry betel nut core is ≤15%.

[0010] The invention discloses an application of betel nut core fermented product in feed. The betel nut core fermented product is added into feed production as feed raw material or feed additive.

[0011] As a further preference of the present invention, when the betel nut core fermentation product is used as a feed raw material or feed additive, its addition form includes mixed feed or drinking water, or various dosage forms made by adding diluents / auxiliaries, and the dosage forms are any one of premixes, powders and aqueous solutions.

[0012] Due to the adoption of the above technical solution, the present invention has the following beneficial effects: 1. The present invention processes and utilizes the discarded betel nut cores in the production of chewing betel nut. On the one hand, the obtained fermented betel nut cores are used as feed raw materials or additives to facilitate the storage of feed; on the other hand, the fermented betel nut cores are used to prepare fermented products after microbial fermentation and applied to animal feed, and are used alone as a raw material for preparing feed additives, or are used together with other feed raw materials. Since a large amount of beneficial bacteria, enzymes, and prebiotics are produced by microbial fermentation, the problem of preserving the betel nut cores and the problem of treating the wastes of chewing betel nut production enterprises are well solved. The use of the betel nut cores for animals is also in line with the national action plan to promote grain saving in the breeding industry; it can also improve animal health, increase feed conversion rate, bring significant economic benefits, and realize the transformation of waste into treasure.

[0013] 2. The fermentation of betel nut core can produce a large amount of beneficial bacteria, enzyme preparations, and oligosaccharide prebiotics after microbial fermentation. Adding them to feed as feed raw materials or additives is beneficial to the intestinal health of animals and can effectively improve the digestion and absorption rate of feed. DETAILED DESCRIPTION

[0014] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0015] A method for preparing a betel nut core fermented product, specifically comprising the following steps: S1. Add brewer's yeast and distilled water in a ratio of 1:5 into a bacterial fermentation tank, then add soybean molasses with a sugar content of 50%, where the soybean molasses accounts for 1% of the total mass of the brewer's yeast and distilled water, and culture at a constant temperature of 35-40° C. with intermittent aerobic stirring for 2-4 hours to obtain a bacterial solution; S2, crushing the wet betel nut core, and evenly adding the bacterial solution prepared in step S1 during the crushing process, wherein the bacterial solution accounts for 0.5% of the mass of the wet betel nut core, to obtain the crushed material to be fermented, wherein the particle size of the crushed material to be fermented needs to be 80% through a 40-mesh sieve, 100% through a 20-mesh sieve, and the moisture content is 35-45%; S3. Add the crushed material to be fermented in step S2 into a fermentation tank or a fermentation bag, seal the fermentation tank or the fermentation bag, and carry out anaerobic fermentation for 48 hours at a constant temperature of 40-55° C. to obtain a wet betel nut core fermentation product, wherein the water content of the wet betel nut core fermentation product is ≥35%.

[0016] The wet fermented betel nut core fermented product obtained in step S3 is dried to obtain a dry betel nut core fermented product, wherein the water content of the dry betel nut core fermented product is ≤15%.

[0017] An application of betel nut core fermentation product in feed, wherein the betel nut core fermentation product is the wet betel nut core fermentation product or the dry betel nut core fermentation product, and the betel nut core fermentation product is added to feed production as a feed raw material or a feed additive. When the betel nut core fermented product is used as a feed raw material or feed additive, its addition form includes mixing with feed or drinking water, or adding diluents / auxiliaries to prepare various dosage forms, and the dosage forms are any one of premixes, powders and aqueous solutions.

[0018] The wet betel nut core fermented product or the dry betel nut core fermented product prepared by the present invention is applied to feed, and a corresponding control test is performed, and the results are as follows: 50 kg of wet betel nut core fermented material is added to 1000 kg of commercial laying hen feed powder, and the mixture is evenly mixed using a mixer to obtain complete laying hen feed powder containing the betel nut core fermented material.

[0019] The above-mentioned layer chicken complete feed powder containing betel nut core fermentation product was used as experimental feed as the experimental group, and the existing commercial layer chicken feed powder was used as the control group. The specific operation was as follows: Experimental animals: 20,000 350-day-old Green Hy-Line Brown laying hens were randomly divided into 2 groups, with 5 replicates in each group and 2,000 hens in each replicate: Test time: April 6 to May 6, 2024, a total of 30 days; Experimental location: Changde, Hunan; Experimental results: Based on the above results, the feed intake and feed conversion rate of animals can be increased by adding betel nut core fermentation products to feed.

[0020] In the present invention, the source of the wet betel nut core is the wet betel nut core discarded in the production of edible chewing betel nut. The betel nut production process is: the green betel nut fruit is washed, steamed, and processed, and then sliced ​​to remove the betel nut core. The betel nut core has a high moisture content. The present invention collects and processes the betel nut core as waste, and applies it to animal breeding as a feed raw material or feed additive. On the one hand, the discharge of waste in the production of edible betel nut is reduced, and the environmental protection pressure of the enterprise is alleviated; on the other hand, the waste is recycled, the resource utilization rate is improved, and the economic benefits are increased, which is in line with the national action plan to promote grain saving in the breeding industry.

[0021] The components contained in the betel nut core were tested, and the test results are as follows:

[0022] From the above test results, it can be seen that betel nut core contains starch, polysaccharides, protein, arecoline, amino acids, etc., and can be used as feed raw material in animal breeding.

Claims

1. A method for preparing betel nut core fermentation product, characterized in that: The specific steps include: S1. Add brewer's yeast and distilled water in a ratio of 1:5 into a bacterial fermentation tank, then add 1% soybean molasses with a sugar content of 50%, and culture at a constant temperature of 35-40°C with intermittent aerobic stirring for 2-4 hours to obtain a bacterial solution; S2, crushing the wet betel nut core, and evenly adding 0.5% of the bacterial solution prepared in step S1 during the crushing process to obtain the crushed material to be fermented, the particle size of the crushed material to be fermented needs to be 80% through a 40-mesh sieve, 100% through a 20-mesh sieve, and the moisture content is 35-45%; S3. Add the crushed material to be fermented in step S2 into a fermentation tank or a fermentation bag, seal the fermentation tank or the fermentation bag, and carry out anaerobic fermentation for 48 hours at a constant temperature of 40-55° C. to obtain the wet betel nut core fermented material.

2. The method for preparing the betel nut core fermented product according to claim 1, characterized in that: The water content of the wet betel nut core fermentation product is ≥35%.

3. The method for preparing the betel nut core fermented product according to claim 1, characterized in that: The wet fermented betel nut core fermented material obtained in step S3 is dried to obtain dry betel nut core fermented material.

4. The method for preparing the betel nut core fermented product according to claim 3, characterized in that: The water content of the dried betel nut core fermentation product is ≤15%.

5. An application of betel nut core fermentation product in feed, characterized in that: The betel nut core fermented product is prepared by the preparation method described in any one of claims 1 to 4, and the betel nut core fermented product is added to feed as a feed raw material or feed additive.

6. The method for preparing the betel nut core fermented product according to claim 5, characterized in that: When the betel nut core fermented product is used as a feed raw material or feed additive, its addition form includes mixing with feed or drinking water, or adding diluents / auxiliaries to prepare various dosage forms, and the dosage forms are any one of premixes, powders and aqueous solutions.

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