Plant injection system comprising actuator and injection tool and use thereof

Abstract

The present invention provides devices and methods for applying a liquid formulation to a plant. An injection system is provided that includes an injection tool operably connected to a fluid delivery device via an actuator. The injection system is configured to deliver a liquid formulation comprising one or more active ingredients to the vasculature of a plant.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority to and the benefit of U.S. Provisional Patent Application No. 63 / 396,559 filed on August 9, 2022 and U.S. Provisional Patent Application No. 63 / 515,499 filed on July 25, 2023, each of which is incorporated herein by reference in its entirety. Technical Field

[0003] The present invention generally relates to devices and methods for administering liquid formulations to plants, and more particularly to an injection system (including an actuator, an injection tool, and a fluid delivery device) for positioning and mounting the fluid delivery device to a plant to dispense a liquid formulation including one or more active ingredients to the plant. Background Art

[0004] Plant injection has been used to administer active ingredients to plants. Traditional plant injection methods may include drilling a borehole in a tree trunk and plugging the borehole with a plug. A needle is inserted through the plug to discharge liquid into the borehole. There is a need in the art for an alternative plant injection system that is easy to install and manufacture on a commercially viable scale. Summary of the invention

[0005] In some aspects, the present invention provides an actuator for connecting an injection tool and a fluid delivery device. Typically, the fluid delivery device includes a tank body containing a liquid formulation, wherein the top of the tank body has a lip, and wherein the fluid delivery device further includes a rod connected to the tank body. In some embodiments, the actuator includes: an activator, wherein the activator is configured to trigger or activate the rod of the fluid delivery device by pressing it, and wherein the activator is configured to be able to install the injection tool; and a frame, wherein the frame includes one or more spreaders, the spreaders press the lip of the fluid delivery device and pull themselves against the lip, wherein the frame has one or more predetermined breaking points, which are configured to be able to break when the activator is pushed downward. In some variations, the activator has a positioning groove, which is configured to receive the injection tool so as to facilitate the precise connection between the actuator and the injection tool. In some variations, the activator includes at least one first locking mechanism, the frame includes at least one second locking mechanism, and at least one first locking mechanism and at least one second locking mechanism engage after the activator is pushed downward to keep the activator in the depressed position.

[0006] In other aspects, an injection tip configured to deliver a liquid formulation into a plant is provided. In some embodiments, the injection tip includes: a cutting edge at a distal end of the injection tip; an injection tip base at a proximal end of the injection tip; a main column extending from the cutting edge to the injection tip base along a central longitudinal axis of the injection tip; at least two side walls extending from each end of the cutting edge to the injection tip base, wherein the cutting edge, side walls and injection tip base form a wedge-shaped body profile extending along the longitudinal axis; opposing faces extending from the injection tip port base and intersecting at the cutting edge; at least two cavities, with at least one cavity on each side of the main column, wherein each cavity is configured as an orifice through the opposing faces; a channel extending through the injection tip base along the central longitudinal axis and terminating at a column portion of the main column, wherein the width of the channel is wider than the column portion of the main column; and a hole extending from the injection tip base upward along the channel through the column portion of the main column. In some variations, the channel is configured to receive the liquid formulation and empty the liquid formulation into the cavity via the aperture.

[0007] In certain of the foregoing embodiments, the main post includes a shoulder proximate the cutting edge and a post proximate the base of the injection tip. In some embodiments, each cavity includes: a primary region at least partially defined by the sidewall and further defined by the shoulder of the main post, wherein the primary region has a maximum longitudinal height; and a secondary region at least partially defined by the shoulder and the post of the main post, wherein the secondary region has a maximum longitudinal height less than the maximum longitudinal height of the primary region.

[0008] In other aspects, an injection tool configured to deliver a liquid formulation into a plant is provided. In some embodiments, the injection tool comprises: any one of the injection tips of the present invention connected to a socket, and the channel of the injection tip is configured to receive the liquid formulation and empty the liquid formulation into the cavity via the hole. In some of the aforementioned variations, the socket is an H-shaped or Y-shaped socket. In other variations, the injection tip is connected to the socket via a sealing area, wherein the sealing area includes a primary seal and an optional secondary seal. In some variations, when a secondary seal is present, the secondary seal is disposed between the primary seal and the socket.

[0009] In other aspects, a plant injection system is provided, comprising: any one of the actuators described in the present invention; any one of the injection tools described in the present invention; and a fluid delivery device. In some variations, the socket of the injection tool is configured to be insertable into the actuator, so that the injection tool is fluidically connected to the fluid delivery device through a connection via the actuator.

[0010] In other aspects, a method for positioning and installing any of the plant injection systems described in the present invention on a plant part is provided. In some embodiments, the method includes: installing the injection tool into the trunk or stem of the plant part; positioning the injection tool by pressing the top beam; and pushing the fluid delivery device so that the predetermined breaking point of the actuator bridge allows the activator to snap into the frame of the actuator.

[0011] In other aspects, a method of using any of the injection tools described in the present invention or any of the injection systems described in the present invention to distribute a liquid formulation to a plant is provided. In some embodiments, the method comprises: piercing the plant with the injection tool; and dispensing the liquid formulation to the plant through the injection tool.

[0012] In certain aspects, methods for regulating the phenotype of one or more plants, or treating plants infected with pathogens, or reducing, controlling and / or eradicating pathogens in plants, or improving abiotic or biotic stress tolerance in plants are provided. In some embodiments, the method comprises: installing any one of the plant injection systems described in the present invention in one or more plants, and applying a liquid formulation with an active ingredient to regulate the phenotype of the plant, or treat plants infected with pathogens, or reduce, control and / or eradicate pathogens in plants, or improve abiotic or biotic stress tolerance in plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present application may be understood by reference to the following description in conjunction with the accompanying drawings.

[0014] Figure 1 An exemplary actuator is shown.

[0015] Figure 2A - Figure 2C is a cross-sectional view of an exemplary actuator mounted to a fluid delivery device.

[0016] Figure 3A and Figure 3B Constraints and loads for a finite element analysis of an exemplary actuator are shown, respectively.

[0017] Figure 4 Shown from Figure 3A and Figure 3B Maximum displacement region of the finite element analysis of an exemplary actuator.

[0018] Figure 5A - Figure 5C Depicted is an exemplary process of installing an injection tool and a fluid delivery device onto a stem or trunk of a plant, the injection tool and the fluid delivery device connected via an exemplary actuator described herein.

[0019] Fig. 6A and Figure 6B Depicted is a cross-sectional view of an exemplary actuator as described herein.

[0020] Fig. 7A - Figure 7C Various views of an exemplary injection tool suitable for use with the actuator described herein are depicted.

[0021] Fig.7D - Figure 7F is mounted to an exemplary actuator Fig. 7A - Figure 7C Cross-section of an injection tool with the exemplary actuator mounted to an exemplary fluid delivery device.

[0022] Figure 7G Depicted is mounting an exemplary injection tool to an exemplary actuator.

[0023] Fig. 8A and Figure 8B An exemplary system of an injection tool positioned in an exemplary actuator connected to a canister having a bag-on-valve insert is depicted.

[0024] Fig.9A An exemplary injection tool is depicted inserted into an exemplary actuator.

[0025] Fig. 9B An exemplary injection tool is depicted inserted into an exemplary actuator connected to a canister having a bag-on-valve insert.

[0026] Fig.10 Another exemplary system of an injection tool positioned in another exemplary actuator coupled to a fluid delivery device is depicted.

[0027] Fig.11A and Fig. 11B A cross section of an exemplary injection tip is depicted.

[0028] Fig. 12A - Fig.12J Various views and perspectives of exemplary injection tools are depicted.

[0029] Fig.13A - Fig. 13C as well as Fig.14A - Fig.14E Other exemplary injection tools are depicted in various views and perspectives.

[0030] Fig.15A - Figure 15G Various views of an exemplary actuator are depicted.

[0031] Fig.16A - Fig. 16C Various views of an example injection tool coupled to an example actuator are depicted. DETAILED DESCRIPTION

[0032] The following description sets forth exemplary systems, methods, parameters, etc. However, it should be appreciated that such description is not intended to limit the scope of the invention but is provided as a description of exemplary embodiments.

[0033] In some aspects, the present invention provides an actuator that can be mounted to a fluid delivery device containing a liquid formulation without triggering the fluid delivery device. In some embodiments, the fluid delivery device includes a tank, such as a tank with a bag-on-valve insert. The actuator of the present invention helps facilitate the automated installation process of the injection tip and the fluid delivery device to deliver the liquid formulation to the plant. In some embodiments, the actuator can be manufactured by injection molding.

[0034] Actuator

[0035] In some embodiments, the actuator includes an activator and a frame. Figure 1 , depicting an exemplary actuator 200. The activator 202 triggers or activates the stem of a fluid delivery device (e.g., a spray can) by pressing it. The activator is configured to mount an injection tool (e.g., an injection tip) that is equipped with a positioning groove 219 on a concave port 216 to ensure a precise connection between the two components. Figure 1 As shown, the frame 201 includes four spreaders that press onto the curled portion of the fluid delivery device and hook themselves onto the curled portion. Figure 1 Element 200a in refers to one of two predetermined breaking points on the frame that will break when the activator is pushed downward. The exemplary activator can be injection molded as one part.

[0036] Figure 2A - Figure 2B It is a tank Figure 1 Cross section of an example of an actuator mounted to a tank. Figure 2A and Figure 2B The actuator is shown in an inactive configuration, wherein Figure 2A is corresponding to Figure 1 The cross section of the line A-A, and Figure 2B is corresponding to Figure 1 The cross section of line B-B. Figure 2C is corresponding to Figure 1 Cross section along line BB of , where the actuator is in the activated configuration.

[0037] The actuator includes a frame 201 for mounting the actuator on a valve cover 208 of a fluid delivery device 207. The frame 201 can be locked in place on the valve cover 208 by one or more spreaders 204, which include hook-like shapes that lock into undercuts in the valve cover 208, which are formed by the crimping process connecting the valve cover 208 to the canister 207. The connection between the spreader 204 and the valve cover 208 has sufficient ridges to withstand axial and angular forces up to a predetermined amount so that the actuator remains on the fluid delivery device 207 during normal use. For example, when the assembly (actuator mounted on a fluid delivery device) is suspended from an injection tip mounted to the actuator, and the longitudinal axis of the assembly is oriented perpendicular to gravity (e.g., as Figure 5A - Figure C), the spreader 204 can be configured to prevent the actuator from falling off the valve cover 208. The actuator can include a second retainer mechanism 206 that is pushed onto the base 210 of the valve cover 208 to further retain the actuator on the fluid delivery device 207 and absorb radially directed forces.

[0038] The actuator includes a bridge 203 that connects the activator 202 to the frame 201. This connection can serve two purposes. First, it holds the unactivated activator 202 in place. Second, it requires a defined force in the axial direction of the fluid delivery device-actuator assembly to prevent accidental discharge of the contents of the fluid delivery device 207. The actuator includes a "total release" activator, meaning that once activated, the entire contents of the fluid delivery device 207 are released in one continuous stream. This is achieved by one or more locking mechanisms 205a of the activator 202, such as Figure 2C As shown, the locking mechanisms 205a are each pushed and held in place by a corresponding locking mechanism 205b of the frame 201. The locking mechanisms 205a / 205b can be constructed so that they are the weakest link of the entire assembly - meaning that they are strong enough to keep the rod 209 of the fluid delivery device 207 depressed (in the activated position), thereby allowing the contents of the fluid delivery device 207 to leave the fluid delivery device, while being weak enough to break in the event of manipulation by excessive force so that the activator 202 can be separated from the frame 201, thereby enabling the rod 209 to return to the inactivated position, which stops the flow of contents, thereby preventing any spillage.

[0039] The actuators described in the present invention may have one or more additional or modified features. For example, FIG. 15A to FIG. 15G Another example actuator 1500 is shown. Fig.15A A perspective view is shown. Fig. 15B A front view is shown. Fig. 15C Describe along Fig. 15B Cross-sectional view along the dotted line A-A. Fig.15DShown along Fig. 15C A cross-sectional view taken along the dotted line B-B. Fig.15E A top view is shown. Fig.15F A bottom view is shown. Figure 15G Describe along Fig.15E Cross-sectional view along the dotted line C-C.

[0040] See also FIG. 15A to FIG. 15G , one modification includes, for example, changing the rib height to achieve actuation visibility. For example, increasing the height of the 6 ribs located on the actuator moving part allows, for example, visual confirmation of actuator activation. In other words, this modification allows the ribs to be seen above the outer actuator body, indicating that the actuator is not activated. If the ribs are not visible above the outer actuator body, the actuator is activated.

[0041] Another modification may include, for example, increasing the height and length of the ribs around the dome. For example, the modification may include increasing the length of the 4× bottom ribs and increasing the length of the dome cup to 2×120 degrees of the actuator moving part. This may provide additional material contact of the actuator moving part with the tank dome when activated, which in turn may help reduce the effect of the tank tilting from the horizontal when fully assembled, filled and inserted into the tree.

[0042] Another modification may include, for example, adding ribs to limit the tilting effect even more. For example, 4× ribs may be added to the inner wall of the outer actuator body. The additional 4 ribs may be located adjacent to the 4× side ribs of the actuator moving part to provide additional support to the actuator when the actuator is activated, thereby reducing the effect of the tank tilting from horizontal when fully assembled, filled and inserted into the tree.

[0043] Performance Standards

[0044] In some variations, when assembled, the injection tool (e.g., injection tip) is located in the actuator and fixed in the internal positioning groove of the actuator. In some variations, removing the injection tool from the actuator will require appropriate force. In some variations, a suitable rotational torque is required to rotate the injection tool in the actuator.

[0045] In some variations, the manufacture of the actuator allows for the injection tool and actuator to be assembled in a manner that does not cause damage to either component.

[0046] In some variations, the injection tool and actuator withstand the forces from a fully assembled fluid delivery device (eg, a 100 ml canister) mounted horizontally and having appropriate weight without mechanical failure or fatigue.

[0047] In some variations, the actuator / tank assembly withstands (at the contact points) the forces from a fully assembled tank mounted horizontally with appropriate weight without mechanical failure or fatigue.

[0048] In some variations, the contact point between the actuator / fluid delivery device assembly requires appropriate rotational torque to rotate the actuator within the collar of the canister of the fluid delivery device.

[0049] In some variations, when assembled, the injection tool is horizontal when fitted into the actuator.

[0050] In some variations, from a fixed point of the injection tool, the actuator / fluid delivery device assembly is subjected to a specific force in all axes before the connection between the actuator and the fluid delivery device fails. If the actuator / fluid delivery device assembly fails, the fluid delivery device is immediately disabled.

[0051] In some variations, to ensure deactivation of the fluid delivery device due to mechanical damage, the failure load of the rod actuation point is less than the failure loads of all other assembly points / potential failure points within the injection tool / actuator / fluid delivery device.

[0052] In other variations, the injection tool / actuator connection is suitably sealed and maintains a seal against back pressure from the slow release of liquid formulation during injection at a maximum starting pressure of at least 1 bar, at least 2 bar, at least 3 bar, at least 4 bar or at least 5 bar.

[0053] In other variations, the actuator / stem connection seals appropriately and maintains a seal against back pressure from the slow release of liquid formulation during injection at a maximum starting pressure of at least 1 bar, at least 2 bar, at least 3 bar, at least 4 bar, or at least 5 bar.

[0054] In other variations, the force required to activate the lever and allow for continued activation is within a suitable force.

[0055] In yet another variation, once the fluid delivery device is activated, activation is maintained throughout the duration of administration of the liquid formulation until the fluid delivery device is empty.

[0056] Business Advantages

[0057] The actuator described in the present invention has several commercial advantages. For example, the actuator is designed for automatic installation. The actuator provides a rigid connection with the injection tool (e.g., injection tip), which helps guide the injection tool with the entire assembly. The injection tool can be pre-installed on the plant together with the fluid delivery device without triggering the fluid delivery device to release its contents.

[0058] The actuator is also designed to provide an appropriate clamping force to securely clamp the fluid delivery device to the plant.In some variations, the actuator includes four spreaders that cannot be easily loosened with a lever.

[0059] The actuator does not require the use of any tubing to connect the injection tool (eg, injection tip) to the fluid delivery device, as the actuator provides a rigid connection between the injection tool and the fluid delivery device.

[0060] The actuator of the present invention can be Figure 5A - Figure 5C The example process shown is for installation. Figure 5A In, an injection tool (e.g., an injection tip) is first installed into the trunk or stem of a plant. The tip is positioned so that there is enough space for the fluid delivery device (e.g., the spray can) and there are no branches in the way. Figure 5B Then, set the injection tool by pressing the top beam. Figure 5C The predetermined breaking point of the actuator bridge allows the activator to snap into the frame.

[0061] Fig. 6A and Figure 6B A cross section of an exemplary actuator 600 is depicted. The actuator 600 includes a frame 601 for mounting the actuator 600 on a fluid delivery device (not shown). The actuator 600 includes a "total release" activator, meaning that once activated, the total contents of the fluid delivery device are released in one continuous stream. This is achieved by one or more locking mechanisms 605a of the activator 602, which are pushed and held in place by corresponding locking mechanisms 605b of the frame 601, respectively. The locking mechanisms 605a / 605b can be configured so that they are the weakest link of the entire assembly - meaning that they are strong enough to keep the rod 609 of the fluid delivery device pressed (in the activated position), thereby allowing the contents of the fluid delivery device to leave the fluid delivery device, while being weak enough to break in the event of manipulation by excessive force, so that the actuator 602 can be separated from the frame 601, thereby enabling the rod 609 to return to the inactivated position, which stops the flow of contents, thereby preventing any spillage. The actuator 600 may include a base portion 606 that may abut a valve cover of a tank (eg, Figure 2A - Valve cover 208 of fluid delivery device 207 of Figure C).

[0062] Injection tools

[0063] Any injection tool that is compatible with the actuator and fluid delivery device described herein may be used. Fig. 7A - Figure 7C An exemplary injection tool suitable for use with the actuators and fluid delivery devices described herein is depicted.

[0064] In some aspects, an injection tool is provided that includes an injection tip, at least a portion of which is designed to penetrate into a plant, such as a stem or trunk of a plant. The injection tip has a channel system (having one or more channels) through which a fluid can flow, and the channel system delivers the fluid to a cavity of the injection tool. In some embodiments, the fluid can enter the cavity through a hole that extends from the base of the injection tip along the channel upward through the middle of the injection tip, such as Figure 7C In other embodiments, the fluid may enter the cavity through a hole or a dispensing port. In some variations, any suitable injection tip and injection tool may be configured for use with the actuators described in the present invention, including the actuators described in WO2020 / 021041 and WO2021 / 152093.

[0065] Fig. 7A - Figure 7C An exemplary design of an injection tip and tool is depicted. Fig.11A , depicting a cross-section of an exemplary injection tip 100, which is Fig. 7A - Figure 7C 100 has a similar design compared to the exemplary injection tip depicted in . The channel 104 extends along the central longitudinal axis through the injection tip base and terminates in the column portion of the main column at the top 102, which has a curvature as shown in the figure, which causes the liquid traveling through the channel 104 to exit through the hole and enter the cavity in a backward direction that is angled compared to the direction in which the liquid travels through the channel. This helps to minimize or prevent clogging of the injection tool. The injection tool 100 can be manufactured by injection molding or additive manufacturing.

[0066] refer to Fig. 12A and Fig. 12B , depicts a front view and a perspective view of an exemplary injection tool 1200, respectively, and describes the components and features of the injection tool in more detail. The injection tool 1000 includes an injection tip 1100 connected to a socket 1200 via a sealing area 1220. The injection tip 1100 includes a cutting edge 1110 at a distal end 1002 of the injection tip and an injection tip base 1120 at a proximal end 1004 of the injection tip.

[0067] The injection tip 1100 also includes a main column 1130 extending from the cutting edge 1110 to the injection tip base 1120 along the central longitudinal axis 1006 of the injection tip. The injection tip 1100 also includes two side walls 1140 extending from each end of the cutting edge 1110 to the injection tip base 1120. The main column 1130 has a shoulder 1132 near the cutting edge 1110 and a column portion 1134 near the injection tip base 1120.

[0068] Each cavity 1160 has a primary region 1164, which is at least partially defined by the sidewall 1140 and further defined by the shoulder 1132 of the main column 1130. The primary region 1164 has a maximum longitudinal height 1164h. Each cavity 1160 has a secondary region 1166, which is at least partially defined by the shoulder 1132 and the column 1134 of the main column 1130. The secondary region 1166 has a maximum longitudinal height 1166h that is less than the maximum longitudinal height 1164h of the primary region 1164.

[0069] The cutting edge 1110, the sidewall 1140 and the injection tip base 1120 form a wedge-shaped body profile extending along the longitudinal axis. The injection tip 1100 has opposing faces 1102a and 1102b extending from the injection tip base 1120 and intersecting at the cutting edge 1110. The injection tip 1100 has two cavities 1160, one on each side of the main column 1130. Each cavity 1160 is configured as an orifice through the opposing faces 1102a and 1102b. The injection tip 1100 also has a channel 1180 that extends through the injection tip base 1120 along the central longitudinal axis 1006 and terminates in the column portion of the main column 1130.

[0070] See also Fig. 12C , which provides a cross-sectional view of the injection tip, the width 1180w of the channel 1180 is wider than the width of the column portion 1134 of the main column 1130. Fig. 12A - Fig. 12C The injection tip 1100 also has a hole 1182 that extends upward from the injection tip base 1120 along the channel 1180 through the column portion 1134 of the main column 1130. The channel 1180 is configured to receive a liquid formulation and empty the liquid formulation into the cavity 1160 through the hole 1182.

[0071] Fig.12D - Fig.12J Other exemplary views of the injection tool are shown. It should be understood that the injection tool can be used Fig.11A and Fig. 12A - Fig.12J A variation of the exemplary injection tip shown in .

[0072] For example, refer to Fig. 11B , depicts another exemplary injection tip 110. In this cross-sectional view, the injection tool 110 includes a dispensing channel 112 that is oriented rearwardly at an angle relative to the direction of liquid travel through the channel, which can minimize or prevent clogging. Due to the relatively more complex geometry and orientation of the channel 112, the injection tool 110 may require additive manufacturing.

[0073] In another example, see Fig.14A - Fig.14E , depicts another exemplary injection tool 1400. The injection tool 1400 has the same features as discussed above. Fig. 12A and Fig. 12B The injection tip depicted in Figure 1 is compared to injection tips with different lumen shapes. In particular, Fig.14A , Fig. 14B and Fig.14E The injection tip is Fig. 12A and 12B A given cavity is not shown to have two distinct regions (i.e., a primary region and a secondary region of the cavity, where these regions have different longitudinal heights). Fig.14A - Fig.14E The injection tip and Fig. 12A and Fig. 12B The injection tips described in share other similar features (e.g., regarding the cutting edge, injection tip base, main column and sidewalls, as well as the positioning of the cavity within the injection tip and the wedge-shaped body profile of the injection tip). The injection tool 1400 has a Y-shaped socket that is connected to the Fig. 12A and Fig. 12B The socket shapes / types are different than the injection tools in the . The variations in socket design are discussed in further detail below.

[0074] In some embodiments, the injection tip of the present invention (including Fig.14A - Fig.14E Compared to Fig. 12A and Fig. 12B The exemplary injection tip described in ) has an average rate of delivery of the liquid formulation into the plant (e.g., tree) of greater than about 50 ml / min. However, it should be understood that the design of the injection tip, including the shape and design of the cavity and / or the channels and ports / orifices that deliver the liquid formulation into the cavity, may have an impact on the average rate of delivery of the liquid formulation into the plant (e.g., tree).

[0075] For example, again referring to Fig.14A - Fig.14E Compared to Fig. 12A and Fig. 12B The shape of the cavity in the injection tip may be a factor affecting the average flow rate of the liquid formulation delivered to the plant using the injection tip. In some variations, it was surprisingly observed that Fig. 12A and Fig. 12B as well as Fig.14A - Fig.14EThe injection tip of the channel / hole design described in has an average flow rate greater than about 50 ml / min, greater than about 100 ml / min, greater than about 150 ml / min, greater than about 200 ml / min or greater than about 225 ml / min; or an average flow rate between about 50 ml / min and 300 ml / min, between 100 ml / min and 250 ml / min, or between 200 ml / min and 250 ml / min.

[0076] Furthermore, it is surprising to observe that Fig. 12A and Fig. 12B The cavity shape of the injection tip shown in FIG. Fig.14A - Fig.14E In some variations, it was surprisingly observed that the injection tip with the cavity shape shown in Fig. 12A and Fig. 12B The injection tip having the cavity shape described in has an average flow rate greater than about 235 ml / min or greater than about 250 ml / min; or an average flow rate between about 235 ml / min and 240 ml / min.

[0077] It should be understood that the size and shape of the various components / features of the injection tip may vary depending on the type, size, maturity of the plant with which the injection tip is designed to be used. Fig.13A - Fig. 13C , depicting three different exemplary injection tools having an H-shaped socket. The three different injection tools have injection tips of different sizes, for example, with respect to the length along the longitudinal axis and / or the width perpendicular to the longitudinal axis, and with respect to the width and size of the injection tip base relative to the socket. Fig.13A and Fig. 13B Compared to the injection tool depicted in Fig. 13C The injection tool depicted in also has different sealing areas / components connecting the injection tip base with the socket.

[0078] exist Fig. 12A - Fig.12J as well as Fig.13A - Fig. 13C In the exemplary injection tool shown, the injection tip is connected to a socket having an H-shape. The H-shaped socket can be configured to be inserted into any actuator described in the present invention, so that the injection tool is fluidly connected to the fluid delivery device through the connection of the actuator.

[0079] For example, Fig.7D - Figure 7F is mounted to the actuator Fig. 7A - Figure 7C Cross-section of an injection tool with the actuator mounted to a fluid delivery device. Fig.7D and Fig. 7E is an orthogonal section showing the assembly in an inactive configuration, and Figure 7F is through and Fig. 7E A cross section of the same plane shows the assembly in an activated configuration.

[0080] Figure 7G An injection tool is shown mounted to an exemplary actuator 702. The injection tool 700 includes one or more locating features 704 that can cooperate with corresponding locating features 706 of the actuator 702 to align the injection tool 700 to the actuator 702. The male port 708 of the injection tool 700 can include one or more outer edges 710 that allow the male port 708 to be pushed into the female port 709 of the activator 712 of the actuator 702 and retained in the female port 709 of the activator 712 by snapping behind a corresponding undercut in the female port 709 (see, e.g., Fig.7D - Figure 7F ). The male port 708 and the female port 709 may be sized to have little or no clearance when mated (e.g., a press fit) so as to maintain a seal. Alternatively, a clearance may be provided and the fit between the one or more outer edges 710 and the associated undercuts may provide a seal when pressed together under the pressure of the fluid outflow during activation.

[0081] The socket of the injection tool 700 is shaped like a sideways H (also referred to herein as an "H-shape") to facilitate placement. The top beam 714 of the H-shaped socket provides a large contact surface for the tree to minimize or avoid damage to the tree, and the top beam can also be used to transfer force to the injection tip 716. The bottom beam 718 of the H-shaped socket can be smaller and designed to pull the injection tip out of the tree, which requires less force than pushing the tip into the tree. The injection tool 700 can be manufactured by additive manufacturing or injection molding.

[0082] The bottom beam 718 of the H-shaped socket of the injection tip 716 can be constructed to provide a thickened sealing surface designed to expand the wood when inserted into the tree and create equal pressure around the tip of the injection tool 700, thus providing a seal. This can improve the reliability and stability of the injection tip 716.

[0083] Fig.9A Another exemplary injection tool / actuator assembly is shown.

[0084] In some variations, other shapes of the socket for the injection tool can be envisioned. Fig.14A - Fig.14E , showing a Y-shaped receptacle having a single port configured to receive a liquid formulation from a fluid delivery device.

[0085] In some embodiments, the injection tools of the present invention are installed in plants with relatively small and large sizes or diameters (e.g., stem or stem diameters). In one example, the portion of the injection tool installed in the plant has a size of about 5 mm or less (e.g., width) and 1 mm or less (e.g., height), and thus these tools are configured to be installed in plants with stems, trunks, roots, branches, etc., with a size of 5 mm or more (e.g., diameter).

[0086] In some embodiments, the size and shape of the piercing portion of the injection tool is designed to minimize damage to the target plant when inserted into the target plant, while maintaining the effective function of the injection tool to deliver the desired dose of liquid formulation directly to the active vascular system of the plant within the desired time period. In some variations, the size and shape of the penetration portion of the injection tip and the injection tip base are collaboratively designed to work together to minimize damage to the target plant while maintaining the effective function of the tip. For example, the length of the injection tip can be selected to be less than the depth of the sapwood in the trunk, and the tool base is constructed with a flange adjacent to the bottom end of the injection tip. In some variations, the size and shape of the flange are arranged to mitigate the risk of inserting the injection tool beyond the end of the adjacent flange of the injection tip and therefore exceeding the inner circumference of the sapwood and entering the heartwood. In some variations, the width of the flange is wider than the widest part of the injection tip. In one example, the multi-port injection tip includes one or more dimensions that are constructed to minimize the damage caused to the plant during installation. The minimal profile of the tip (as well as other tip embodiments described herein) minimizes damage to plants compared to larger profile devices including syringes, plugs, stoppers, or the like, which measure approximately 7 mm (7.14 mm in one example) and are a full 2 ​​mm larger than the example tip. Thus, the potential for tree damage is reduced, and the potential for fungal, bacterial, and insect intrusion is minimized (e.g., reduced or eliminated). In one example, the tip described herein as well as other tip examples are readily used with plants having stems, trunks, branches, etc., with diameters greater than 4.68 mm, including, but not limited to, fruit trees, nut trees, berry bushes, flowering plants, and trees and forests.

[0087] In certain embodiments, the selected injection tool allows for precise delivery (also referred to as "precision injection") of the formulation into the plant. Precision delivery refers to delivering the formulation only or substantially only to a target location in the plant. For example, in some embodiments, the target location is the living vasculature of a tree. In some variations, the living vasculature of the tree is xylem and / or phloem. In other embodiments, precisely delivering the liquid formulation comprises inserting the injection tool so that the dispensing reservoir is within the living vasculature of the plant and does not extend beyond the living vasculature.

[0088] In some embodiments, the actuator may further include a sealing component, such as an O-ring, at the junction between the actuator and the socket of the injection tool. For example, the actuator described herein may be modified around the tip socket of the actuator moving component to create an initial increased diameter cavity for the injection tool rod location, wherein the remaining cavity below the increased injection tool rod diameter is used to optionally place a sealing component (such as an O-ring) to provide additional resistance against potential leakage during the tree injection process. FIG. 16A to FIG. 16C An exemplary injection tool 1602 is depicted coupled to an exemplary actuator 1604 , wherein the actuator 1604 includes an o-ring 1606 in contact with the injection tool 1602 . Fig.16A Describe along Fig. 16C Cross-sectional view along the dotted line B-B. Fig. 16B Describe along Fig. 16C Cross-sectional view along the dotted line A-A. Fig. 16C A top view is shown.

[0089] Fluid delivery device

[0090] Any suitable fluid delivery device can be used with the actuator and injection tool described in the present invention and used in the injection system described in the present invention.In some embodiments, the fluid delivery device includes a canister. Fig. 8A An exemplary system 800A is depicted that includes an injection tool 804A, an actuator 806A, and a canister 807A. Fig.9A An injection tool 900 is shown connected to an actuator 902A.

[0091] In some embodiments, the can has a bag-on-valve insert. See, for example, Figure 8B , the valve-on-bag insert is connected to a rod of a socket that receives an injection tool. Figure 8B An exemplary system 800B is depicted that includes an injection tool 804B, an actuator 806B, and a canister 807B. A bag-on-valve insert (not labeled) is connected to a bag-on-valve insert stem 802B. Fig. 9B An injection tool 900 is depicted connected to an actuator 902B, as well as a bag-on-valve insert (not labeled, within the canister) and a delivery device 907 .

[0092] In one variation, the fluid delivery device is a spray can.

[0093] In some embodiments, the fluid delivery device comprises any suitable liquid formulation and active ingredient, including those described below.

[0094] Injection system

[0095] In some aspects, the present invention provides an injection system comprising: an injection tool, an actuator and a fluid delivery device. In some variations, the injection tool is connected to the actuator via a socket extending from the base of the tool body of the injection tool and is configured to connect to a rod of the fluid delivery device. The injection tool is fluidically connected to the fluid delivery device via a connection via the actuator. The term "fluid connection" refers to a connection capable of transferring a fluid, particularly a connection from a fluid delivery device to an injection tool.

[0096] Fig. 8A , Figure 8B and Fig. 9B An exemplary injection system is depicted in which an injection tool is connected to an actuator via a rod, and the actuator is mounted to a fluid delivery device.

[0097] Fig.10 Another exemplary injection system 950 is depicted having a different exemplary actuator 952 connected to a delivery device 957. The actuator is shown in the figure connected to the injection tool 954 horizontally.

[0098] Liquid preparations

[0099] Any suitable liquid formulation can be used in the injection system described herein. In some embodiments, the liquid formulation is water soluble. In some variations, the liquid formulation comprises nutrients. In some variations, the liquid formulation comprises micronutrients. In some variations, the liquid formulation is a semi-liquid formulation. In some variations, the liquid formulation is a gel formulation. In some variations, the liquid formulation is delivered as a semi-liquid or gel formulation.

[0100] In certain embodiments, liquid preparation comprises one or more active components.In some modifications, for example, by mixing active component with one or more suitable additives (for example suitable extender, solvent, spontaneous promoter, carrier, emulsifier, dispersant, antifreeze agent, biocide, thickener, adjuvant etc.) to prepare preparation.In the present invention, adjuvant is the component of the biological effect that strengthens preparation, and component itself does not have biological effect.The example of adjuvant is the reagent that promotes to retain, diffuse or penetrate in target plant.One embodiment of the present invention is included in the long-term supply active component to plant in the growing season, and wherein auxiliary agent is stabilizer (for example low temperature stabilizer), preservative, antioxidant, light stabilizer or improves other reagents of chemical and / or physical stability.

[0101] Examples of typical liquid formulations include water-soluble liquids (SL), emulsifiable concentrates (EC), aqueous emulsions (EW), suspension concentrates (SC, SE, FS, OD), water-dispersible granules (WG) and fluids (which include one or more of liquids, gases, gels, vapors, aerosols, etc.). These and other possible types of formulations are described, for example, in Croplife International and Pesticide Specifications, Manual on the Development and Use of FAO and WHO Pesticide Specifications, FAO Plant Production and Protection Papers (prepared by the Joint FAO / WHO Pesticide Specifications Meeting, 2004, ISBN: 9251048576); "Catalogue of pesticide formulation types and international coding system" Technical Monograph No. 2, Sixth Edition, May 2008, Croplife International.

[0102] In some embodiments, the composition is prepared in a known manner, such as Mollet and Grubemann in Formulation Technology (Wiley VCH, Weinheim, 2001); or Knowles in New Developments in Crop Protection Production Formulations (Agrow Reports DS243, T&F Informa, London, 2005). For example, the formulation is prepared by mixing the active ingredient with one or more suitable additives (such as suitable extenders, solvents, spontaneous promoters, carriers, emulsifiers, dispersants, antifreeze agents, biocides, thickeners, adjuvants, etc.). In the present invention, an adjuvant is a component that enhances the biological effect of the formulation, while the component itself does not have a biological effect. Examples of adjuvants are agents that promote retention, diffusion or penetration in the target plant. One embodiment of the present invention includes long-term supply of active ingredients to plants during the growing season, wherein the adjuvant is a stabilizer (e.g., a low-temperature stabilizer), a preservative, an antioxidant, a light stabilizer, or other agents that improve chemical and / or physical stability.

[0103] Examples of suitable adjuvants are solvents, liquid carriers, surfactants, dispersants, emulsifiers, wetting agents, adjuvants, solubilizers, penetration enhancers, protective colloids, wetting agents, repellents, attractants, feeding stimulants, compatibilizers, bactericides, antifreeze agents, defoamers, colorants, stabilizers or nutrients, UV protectants, tackifiers and / or adhesives. Specific examples of each of these adjuvants are well known to those of ordinary skill in the art, see, for example, US2015 / 0296801A1.

[0104] The composition may optionally include 0.1-80% of a stabiliser and / or nutrient and 0.1-10% of a UV protectant.General examples of suitable proportions for the various formulation types mentioned above are given in Agrow Reports DS243 (T&F Informa, London, 2005).

[0105] At certain application rates, the compositions and / or formulations according to the invention may also have a strengthening effect in plants. "Plant strengthening" (resistance-inducing) substances are understood in the present invention to mean those substances or combinations of substances which are able to stimulate the plant defense system in such a way that, when subsequently inoculated with harmful microorganisms, the treated plants display a significant degree of resistance to these microorganisms.

[0106] In some embodiments, when the active ingredient is applied, the application can be continuous over a long period of time or interval. In some variations, the application can also be connected to a disease monitoring system and triggered "on demand". In some variations, the formulation can include 0.5% to 90% by weight of the active compound based on the weight of the formulation.

[0107] Many active ingredients can be used in the injection system of the present invention. The active ingredients specified by the "common name" of the active ingredient in the present invention are known and described in, for example, the Pesticide Handbook (18th edition, edited by Dr. J. A. Turner (2018), which includes herbicides, fungicides, insecticides, acaricides, nematicides, plant growth regulators, anthelmintics, synergists and other agents).

[0108] application

[0109] In some embodiments, the present invention provides a process for regulating the phenotype of one or more plants by installing a plant injection system according to the present invention in one or more plants and implementing a liquid formulation with active ingredients for regulating the phenotype of the plant. In other embodiments, the present invention provides a method for regulating plant phenotypes, for example, treatment, prevention, protection and immunity, which means that the plant is locally and overall resistant to pathogenic attacks and pest attacks. The injection tool of the present invention distributes the liquid formulation directly to the interior of the plant without spraying, and there is no corresponding loss of the wrong application of the spray formulation. The subject matter of the present invention makes the formulation directly contact the plant tissue, and in some embodiments, the formulation is selectively implemented at the appropriate time to minimize (e.g., eliminate or minimize) the accumulation of chemical residues in the managed fruit or crop. For example, the present invention includes injection methods, devices and systems for treating plants whose xylem and / or phloem may be attacked by bacteria, fungi, viruses and / or other pathogens; and / or injection methods, devices and systems for controlling bacteria, fungi, viruses and / or other pathogens that invade the xylem and / or phloem of plants.

[0110] plant

[0111] "Plants" refers to all plants and plant populations, such as desired and undesired wild plants, cultivars and plant varieties (whether or not protected by plant variety or plant breeder's rights). Cultivar varieties and plant varieties may be plants obtained by conventional propagation and breeding methods, which may be assisted or supplemented by one or more biotechnological methods, such as by the use of double haploids, protoplast fusion, random and directed mutagenesis, molecular or genetic markers or by bioengineering and genetic engineering methods. "Plants" include whole plants and parts thereof, including but not limited to branch vegetative organs / structures (such as leaves, stems and tubers), roots, flowers and flower organs / structures (such as bracts, sepals, petals, stamens, carpels, anthers and ovules), seeds (including embryos, endosperms and seed coats) and fruits (mature ovaries), plant tissues (such as vascular tissues, ground tissues, etc.) and cells (such as guard cells, egg cells, etc.) and their progeny. "Fruit" and "plant products" should be understood as any plant product that is further utilized after harvest, such as true fruits, nuts, wood, etc., i.e., any economic value produced by the plant.

[0112] In some variations, the plants that may benefit from application of the products and methods of the present invention are selected from tree crops (e.g., walnuts, almonds, pecans, hazelnuts, pistachios, etc.), citrus trees (Citrus spp., i.e., for example, oranges, lemons, grapefruits, mandarins, etc.), fruit crops (e.g., pome, stone fruit or berries, such as apples, pears, plums, peaches, cherries, etc.), vine crops (e.g., grapes, blueberries, blackberries, etc.), coffee (Coffee spp.), coconut (Cocos iiucifera), pineapple (Ananas comosus), cocoa (Camellia sinensis), bananas (Musa spp.), laurel plants (e.g., avocado (Persea americana), cinnamon or camphor), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), natural rubber trees, date palms, oil palms, ornamentals, forestry (e.g., pine, spruce, eucalyptus, poplar, conifers, etc.), and / or boxwood.

[0113] Conifers useful in practicing the embodiments are selected from pines, such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pinus radiata); Douglas fir (Pseudotsuga menziesii); western hemlock (Tsuga canadensis); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs, such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars, such as western red cedar (Thuja plicata) and / or Alaskan yellow cedar (Chamaeeyparis nootkatensis).

[0114] Palm trees that can be treated are selected from Archontophoenix alexandrae (Alexander King Palm), Arengaspp. (Dwarf Sugar Palm), Borassus flabellifer (Solanum nigrum), Brahea armata (Blue Palm), Brahea edulis (Guadalupe Palm), Butia capitate (Needle Palm), Chamaerops humilis (European Fan Palm), Carpentaria spp. (Carpentaria Palm), Chamaedorea elegans (Parlor Palm), C.erupens (Bamboo Palm), C.seifrizii (Reed Palm), Chrysalidocarpus lutescens (Areca Palm), Coccothrinaxargentata (Silver Palm), C.crinite (Old Man Palm), Cocos nucifera (Coconut Palm), Elaeisguineensis (African Oil Palm), Howea forsterana (Kentia Palm), Livistona rotundifolia (Round-leaved Fan Palm), Neodypsis decaryi (Triangle Palm); Normanbya normanbi (Queensland black palm); Pinanga insignis; Phoenix canariensis (Canary Island date palm); Ptychosperma macarthuri (MacArthur palm); Rhopalostylis spp (shaving brush palm); Roystonea elata (Florida royal palm), R. regia Cuban (royal palm), Sabal spp (cabbage / dwarf palm), Syagrus romanzoffiana (queen palm), Trachycarpus fortune (windmill palm), Trythrinax acanthocoma (spiny fiber palm), Washingtonia filifera (petticoat palm), and / or W. robusta (Washington / Mexican fan palm). One embodiment includes preventing or treating palm tree bud rot caused by, for example, Phytophthora, Trichosperma mirabilis, and / or bacteria. Unlike most trees that have many new growth points, palm trees rely on their single terminal bud. If the terminal bud or heart bud becomes diseased and dies, the tree will not be able to grow new leaves and will eventually die. Therefore, preventive care is necessary to keep your palm tree healthy.

[0115] Diseases

[0116] One embodiment includes a method of reducing damage to plants and / or plant parts or loss of harvested fruit or plant products caused by plant pathogenic fungi by controlling the plant pathogenic fungi, comprising applying the tool, system, agent / formulation or method of the present invention to the plant. In some variations, the injection system described herein can be used to control, prevent or treat the following fungal plant diseases selected from the group consisting of: Botrytis cinerea on fruits and berries (e.g., strawberries), rapeseed, grapevines, forest plants, cinerea (teleomorph: Botryotiniafuckeliana: gray mold); Ceratocystis (synonym Ophiostoma) (rots or wilts) on broadleaved and evergreen trees, e.g. C. ulmi (Dutch elm disease) on elms; Cercospora (cercospora leaf spot) on coffee; Colletotrichum (teleomorph: Glomerella) (anthracnose) on soft fruit; Cycloconium, e.g. C. oleaginum on olive trees; Cycloconium (C. oleaginum) on fruit trees, grapevines (e.g. C. liriodendri, teleomorph: Neonectrialiriodendri: blackfoot disease) and ornamental trees lindrocarpon) (e.g. fruit rot or grapevine blackfoot, teleomorphs: Nectria or Neonectria); Esca (vine wilt, dry blight) on grapevines caused by Formitiporia (syn. Phellinus) punctata, F. mediterranea, Phaeomoniella chlamydospora (formerly Phaeoacremonium chlamydosporum), Phaeoacremonium aleophilum and / or Botryosphaeria obtuse; pome fruit (E. pyri), soft fruit (E. veneta: anthracnose) and grapevine (E.ampelina: anthracnose) on fruit trees; Eutypalata (Eutypa canker or wilt, anamorph: Cytosporinalata, synonym Libertellablepharis) on fruit trees, grapevines and ornamental trees; Fusarium (anamorph: Gibberella) on various plants (wilt, root rot or stem rot); Glomerella cingulata on grapevines, pome fruit and other plants; Guignardia bidwellii (black rot) on grapevines; Gymnosporangium on rosaceae and junipers, such as G. sabinae (rust) on pears; Hemileia, such as H. vaportrix (coffee leaf rust) on coffee; Isariopsis on grapevines. clavispora) (synonym Cladosporium vitis); Monilinia, e.g. M. laxa, M. fructicola and M. fructigena (blossom and twig rot, brown rot) on stone fruits and other Rosaceae; Mycosphaerella on bananas, soft fruits, e.g. M. fijiensis (Sigatoka black spot) on bananas; Phialophora, e.g. on grapevines (e.g. P. Tracheiphila and P. tetraspora); Grapevines (e.g. P. viticola: Phomopsis on stalk and leaf spot (root stalk and leaf spot); Phytophthora (wilt, root rot, leaf rot, stem rot and fruit rot) on various plants such as broad-leaved trees (e.g. P. ramorum: sudden oak death); Plasmopara, e.g. P. viticola (grapevine downy mildew) on grapevines; Podosphaera (powdery mildew) on rosaceae, hops, pome and soft fruits, e.g. P. leucotricha on apples; Pseudopezicula tracheiphila (grape leaf scorch or 'rotbrenner', anamorph: Phialophora) on grapevines; Ramularia, e.g. R.collo-cygni (Physiological leaf spot) and R. Beticola on sugar beet; Rhizoctonia on cotton, rice, potatoes, turf, corn, rapeseed, potatoes, sugar beet, vegetables and various other plants, such as R. solani (root / stem rot) on soybeans, R. solani (shear blight) on rice or R. Cerealis (wheat sheath blight) on wheat or barley; Rhizopus graminearum on grape vines. stolonifer) (black mold, soft rot); Uncinula (synonym Erysiphe) necator (powdery mildew, anamorph: Oidiumtuckeri) on grapevines; Taphrina, such as T. deformans (leaf curl) on peaches and T. pruni (plum sac) on plums; Thielaviopsis (black root rot) on pome fruits; Apples (e.g. V. inaequalis) and Venturia (black scab) on pears; and Verticillium (wilt) on various plants such as fruit and ornamental trees, grapevines, and soft fruits.

[0117] In some variations, the injection systems of the present invention can be used to control, prevent or cure diseases in plants selected from:

[0118] Apple diseases: Blossom blight (Monilinia mali), powdery mildew (Podosphaera leucotricha), Alternaria leaf spot / Alteraria alternata apple pathotype, scab (Venturia inaequalis), bitter rot (Colletotrichum acutatum), anthracnose (Colletotrieiium acutatum), rot (Valsa ceratosperma), and / or crown rot (Phytophtora cactorum);

[0119] Pear diseases: scab (Venturianashicola, V. pirina), black spot / purple spot (Alternaria alternate Japanese pear pathotype), rust / frog eye (Gymnosporangium haraeanum), and / or Phytophtora fruit rot (Phytophtora cactorum);

[0120] Peach diseases: Brown rot (Monilinia fructicola), black spot / scab (Cladosporiumcarpophilum), and / or Phomopsis sp.;

[0121] Grapevine diseases: Anthracnose (Elsinoe ampelina), powdery mildew (Uncinula necator), ripe rot (Glomerella cingulata), black rot (Guignardia bidwellii), downy mildew (Plasmoparaviticola), rust (Phakopsora ampelopsidis), and / or gray mold (Botrytis cinerea);

[0122] Japanese persimmon diseases: anthracnose (Gloeosporium kaki) and / or leaf spot (Cercospora kaki, Mycosphaerella nawae);

[0123] Cruciferous vegetable diseases: Alternaria japonica, Cercosporella brassicae, and / or Peronospora parasitica; oilseed rape diseases: Sclerotinia sclerotiorum and / or Alternaria brassicae;

[0124] Rose diseases: black spot (Diplocarponrosae) and / or powdery mildew (Sphaerothecapannosa);

[0125] Banana diseases: Mycosphaerella fijiensis, Mycosphaerella musicola, Pseudocercospora musae; and / or Colletotrichum musae, Armillaria mellea, Armillaria tabescens, Pseudomonassolanacearum, Phyllachora musicola, Mycosphaerella fijiensis, Rosellinia bunodes, Pseudomonas spp., Pestalotiopsis leprogena, Cercospora hayi, Pseudomonassolanacearum, Ceratocystis paradoxa, Verticillium theobromae, Trachysphaera fructigena, Cladosporium musae, Junghuhnia vincta, Cordanajohnstonii, Cordana musae, Fusariumpallidoroseum, Colletotrichum musae, Verticillium theobromae, Fusarium, Acremonium, Deightoniella torulosa, Nattrassia mangiferae, Dreschslera gigantean, Guignardia musae, Botryosphaeria ribis, Fusariumsolani, Nectria haematococca, Fusarium oxysporum, Rhizoctonia, Colletotrichum musae, Uredo musae, Uromyces musae, Acrodontium simplex, Curvularia eragrostidis, Drechslera musae-sapientum, Leptosphaeria musarum, Pestalotiopsis disseminate, Ceratocystisparadoxa), Haplobasidion musae, Marasmiellus inoderma, Pseudomonassolanacearum, Radopholus similis, Lasiodiplodiatheobromae, Fusarium pallidoroseum, Verticilliumtheobromae, Pestalotiopsis palmarum, Phaeoseptoria musae, Pyricularia grisea, Fusariummoniliforme, Gibberellafujikuroi, Erwinia carotovora, Erwinia chrysanthemi, Cylindrocarpon musae, Meloidogyne arenaria), Meloidogyne incognita, Meloidogyne javanica, Pratylenchus coffeae, Pratylenchus goodeyi, Pratylenchus brachyurus, Pratylenchus reniformia, Sclerotinia sclerotiorum, Nectriafoliicola, Mycosphaerella musicola, Pseudocercospora musae, Limacinula tenuis, Mycosphaerella musae, Helicotylenchus multicinctus, Helicotylenchus dihystera, Nigrospora sphaerica, Trachysphaerafrutigena, Ramichloridium musae, Verticillium theobromae;

[0126] Citrus fruit diseases: black spot (Diaporthe citri), scab (Elsinoe fawcetti) and / or fruit rot (Penicillium digitatum, P. italicum);

[0127] Tea diseases: Exobasidium reticulatum, Elsinoe leucospila, Pestalotiopsis sp., Colletotrichum theaesinensis;

[0128] Palm tree diseases: bud rot, crown rot, red ring, Pudricion de Cogollo, lethal yellows;

[0129] Box tree diseases: Cylindrocladium buxicola (also called Calonectria pseudonaviculata), Volutella buxi, Fusarium buxicola.

[0130] The methods of the present invention can be used to reduce damage caused by various insect pests. The target insects can be selected from the following orders: Lepidoptera, Coleoptera, Diptera, Thysanoptera, Hymenoptera, Orthoptera, Acarina, Siphonaptera, Thysanura, Chilopoda, Dermaptera, Phthiraptera, Hemipteras, Homoptera, Isoptera, and / or Aptero. Examples of such pests include, but are not limited to, arthropods, such as Lepidoptera (e.g., Plutellidae, Noctuidae, Pyralidae, Tortricidae, Lyonetiidae, Carposinidae, Gelechiidae, Crambidae, Arctiidae, and / or Lymantriidae), Hemiptera (e.g., Cicadellidae, Delphacidae, Psyllidae, Aphididae, Aleyrodidas, Orthezidae, Miriidae), and / or The invention further includes the following families: the order of the order Coleoptera (e.g., Scarabaeidae, Elateridae, Coccinellidae, Cerambycidae, Chrysomelidae, and / or Curculionidae), the order of the order Diptera (e.g., Muscidae, Calliphoridae, Sarcophagidae, Anthomyiidae, Tephritidae, Opomyzoidea, and / or Carnoidea), the order of the order Orthoptera (e.g.,The present invention also includes family A. spp. (Acrididae, Catantopidae, and Pyrgomorphidae), Thysanoptera (e.g., Thripidae, Aeolothripidae, and Merothripidae), Tylenchida (e.g., Aphelenchoididae and / or Neotylechidae), Collembola (e.g., Onychiurus and Isotomidae), Acarina (e.g., Tetranychidae, Dermanyssidae, Acaridae, and / or Sarcoptidae), Spodoptera (e.g., Spodoptera), and / or Psoralea (e.g., Psoralea), tylommatophora) (e.g., Philomycidae and / or Bradybaenidae), Ascaridida) (e.g., Ascaridida and / or Anisakidae), Opisthorchiida, Strigeidida, Blattodea) (e.g., Blaberidae, Cryptocercidae and / or Panesthiidae), Thysanura) (e.g., Lepismatidae, Lepidotrichidae and / or Nicoletiidae), and / or Boxwood Moth / Box Tree Caterpillar (Cydalima perspectalis).

[0131] The injection system of the present invention may also be used to combat bacterial pathogens that attack, consume (in whole or in part) or arrest the growth and / or development of plants and / or act as a vector for the delivery of diseases caused by such bacterial pathogens to the plants and / or other plants. Bacterial pathogens can include Agrobacterium, Agrobacterium tumefaciens, Erwinia, Erwinia amylovora, Xanthomonas, Xanthomonas campestris, Pseudomonas, Pseudomonas syringae, Ralstonia solanacearum, Corynebacterium, Streptomyces, Streptomyces scabies, Actinobacteria, Micoplasmas, Spiroplasmas, and / or Fitoplasmas.

[0132] The injection system of the present invention can also be used to reduce, control and / or eradicate viral pathogens that attack, consume (in whole or in part) or prevent the growth and / or development of plants and / or act as a vector of transmission to plants and / or other plants caused by such viral pathogens. Such viral pathogens can include Carlaviridae, Closteroviridae, viruses that attack citrus fruit, Cucumoviridae, Ilarviridae, dwarf viruses that attack plums, Luteoviridae, Nepoviridae, Potexviridae, Potyviridae, Tobamoviridae, Caulimoviridae, and other viruses that attack vegetation and crops.

[0133] Plant growth regulating compounds can be used for example to inhibit the vegetative growth of plants. This growth inhibition has economic significance, for example, near roadsides and pipelines or overhead cables, or very generally do not want the growth of herbaceous plants and woody plants in areas where plants grow vigorously. Inhibiting vegetative plant growth can also lead to increased yields, because nutrients and assimilates are more beneficial to the vegetative parts of plants than to the formation of flowers and fruits. Usually, growth regulators can also be used to promote vegetative growth. This is very beneficial when gathering vegetative plant parts. However, promoting vegetative growth can also promote reproductive growth, because more assimilates are formed, resulting in more or larger fruits.

[0134] Use growth regulators to control the branching of plants. On the one hand, by breaking the apical bud dominance and combining with growth inhibition, it is possible to promote the development of lateral branches, which is also very necessary in the cultivation of ornamental plants. However, on the other hand, the growth of lateral branches can also be suppressed. For example, in the cultivation of tobacco or in the cultivation of tomatoes, this effect is particularly interesting. Under the influence of growth regulators, the amount of leaves on the plant can be controlled so that the defoliation of the plant is achieved at the desired time. This defoliation plays a major role in the mechanical harvesting of cotton, but is also conducive to promoting the harvesting of other crops (for example grape cultivation).

[0135] Growth regulators can also be used to achieve faster or delayed ripening of the harvested material before or after harvesting. This is particularly advantageous because it allows an optimal adjustment to market requirements. In addition, growth regulators can improve fruit color in some cases. In addition, growth regulators can also be used to concentrate ripening within a certain period of time. This establishes the prerequisite for fully mechanical or manual harvesting in a single operation (for example in coffee).

[0136] By using growth regulators, it is also possible to influence the dormancy of seeds or shoots of plants, causing plants (including pineapples or ornamental plants in a nursery, for example) to sprout, shoot or flower when they would not normally tend to do so.

[0137] In addition, growth regulators can induce resistance in plants to frost, drought or high salinity in the soil. This allows the cultivation of plants in areas that are normally unsuitable.

[0138] Compositions and / or formulations according to the present invention also show effective strengthening effects in plants. Therefore, they can be used to mobilize plant defense against undesirable microbial attacks. In the present invention, plant strengthening (resistance induction) substances are understood to mean those substances that can stimulate the defense system of plants in such a way that when subsequently inoculated with undesirable microorganisms, the treated plants form a high degree of resistance to these microorganisms. The active compounds according to the present invention are also suitable for increasing the yield of crops. In addition, they show reduced toxicity and are well tolerated by plants.

[0139] In addition, in the context of the present invention, plant physiological effects include the following (all of which can be regulated by the compositions, methods and devices provided by the present invention): abiotic stress tolerance, including temperature tolerance, drought tolerance and recovery after drought stress, water use efficiency (associated with reduced water consumption), flood tolerance, ozone stress and UV tolerance, tolerance to chemicals such as heavy metals, salts, pesticides (safeners) and the like; and biotic stress tolerance, including increased resistance to fungal diseases, increased resistance to nematodes, viruses and bacteria; and increased plant vigor, including plant health, plant quality, seed vigor, reduced damping-off, improved appearance, increased recovery, improved greening effect and improved photosynthetic efficiency.

[0140] Furthermore, the injection system according to the invention can be used to reduce the mycotoxin content in harvested material and in food and feed prepared therefrom.

[0141] In another embodiment of the present invention, the injection system of the present invention can be used to provide plants with nutrient elements such as nitrogen, phosphorus and potassium, as well as mineral elements including but not limited to silicon, calcium, magnesium and manganese.

[0142] In some embodiments, methods are provided for treating plants whose xylem or phloem, or both, are invaded or are at risk of being invaded by bacteria, fungi, viruses, and / or other pathogens using the injection systems described herein. In some embodiments, the methods increase the strength of the plant to resist bacterial attack. In some variations, the methods enhance the infected plant or improve plant health recovery in an infected plant.

[0143] In some embodiments, the present invention provides methods for improving the strength of plants infected by Xylella fastidiosa, which is a plant bacterium confined to the xylem that is believed to cause the disease. In certain embodiments, the present invention provides methods for enhancing or maintaining the health of olive trees. In some embodiments, the present invention provides methods for treating olive rapid decline syndrome in olive trees. In some variations, the present invention provides methods for improving the strength of olive trees infected by Xylella fastidiosa subspecies pauca. In other variations, the present invention provides methods for improving the strength of olive trees infected by Xylella fastidiosa subspecies multiplex.

[0144] In other embodiments, the present invention provides methods for improving the strength of olive trees infected with Xylella fastidiosa, Xylella fastidiosa multiplex, Xylella fastidiosa sandyi, and / or Xylella fastidiosa pauca. For example, in some variations, methods are provided for improving the strength of grapevines infected with Xylella fastidiosa. In some variations, methods are provided for improving the strength of citrus trees infected with Xylella fastidiosa pauca. In some variations, methods are provided for improving the strength of stone fruit trees infected with Xylella fastidiosa multiplex. In one variation, methods are provided for improving the strength of cherry, plum, peach, and / or almond trees infected with Xylella fastidiosa multiplex.

[0145] In some embodiments, the present invention provides methods for enhancing or maintaining plant health in citrus plants and citrus plant groves. In some such embodiments, the present invention provides methods for treating diseased plants and / or methods for controlling bacteria, fungi, viruses and / or other pathogens that cause citrus greening disease in citrus plants. In other such embodiments, the methods provided by the present invention are used to treat citrus plants whose xylem and / or phloem have been invaded by pathogenic bacteria, fungi, viruses and / or other pathogens, to control pathogenic bacteria, fungi, viruses and / or other pathogens that cause diseases, and to prevent diseases by preventing diseases caused by pathogenic pathogens such as bacteria, fungi and viruses from fully infecting plants.

[0146] In some embodiments, using the systems, devices and methods of the present invention to control citrus greening disease in citrus plants includes reducing the bacterial concentration (titer) in the vasculature. In some variations, using the systems, devices and methods of the present invention to control citrus greening disease in citrus plants includes reducing the bacterial concentration (titer) in the vasculature by enhancing the plant's natural defense system. In certain embodiments, the systems, devices and methods of the present invention can provide treatments that result in suppressing the disease to a level that restores citrus yield. In some variations, bacterial titer refers to the bacterial concentration in the vasculature of an infected plant. Bacterial titer can be measured using any suitable method and technique known in the art. For example, in one variation, bacterial titer is measured by quantitative PCR. In one variation, CLas titer is measured, for example, using any suitable technique known in the art.

[0147] In some embodiments, the citrus plant is a citrus tree or a citrus shrub. In some variations, the citrus tree is an orange tree, a lemon tree, a lime tree, a grapefruit tree, or a pomelo tree. In certain variations, the citrus plant is a lemon shrub or a lime shrub. In one variation, the citrus shrub is a dwarf citrus shrub. In other variations, the citrus tree is a mature tree.

[0148] In some variations, the citrus plants are afflicted with citrus greening disease caused by L. asiaticus, L. africanus, L. americanus. In some variations, the disease is spread by the Asian citrus psyllid Diaphorina citri and the African citrus psyllid Trioza erytrea.

[0149] In some embodiments, the infected citrus plants exhibit at least one symptom caused by citrus greening disease. In some embodiments, the citrus plants to which the injection formulation is applied are infected. In some embodiments, the citrus plants to which the injection formulation is applied are not infected. In some embodiments, the methods described herein are used only for citrus plants having one or more symptoms caused by citrus greening disease. Such symptoms may include any one or more of the following: asymmetric yellowing of veins and adjacent tissues; blotchy spots on entire leaves; premature defoliation; dieback of young branches; rootlets and lateral root rot; decreased vigor; stunted growth and production of multiple out-of-season flowers; production of small, irregularly shaped fruits with thick, pale rind that remains green at the bottom and tastes bitter.

[0150] Example

[0151] The presently disclosed subject matter will be better understood by reference to the following examples, which are provided by way of illustration of the invention and not by way of limitation.

[0152] Example 1: Finite Element Analysis

[0153] This example uses finite element analysis to test the displacement and stress of an exemplary actuator, known as actual deformation. The actuator used in this example is Figure 3A , Figure 3B and Figure 4 300. The actuator 300 includes an activator 302 and a frame 301. The activator is configured to mount an injection tool, which is equipped with a positioning groove 306 on a concave port 309 to ensure a precise connection between the activator and the injection tool. Figure 3B The load box of the actuator includes four fixed constraints at the spreader 304, and applies the load to the top where the rod of the pressurized spray can is located. The load is a linear force of 30N, which is the maximum force that can be achieved by the rod of the spray can used. The exemplary actuator used in this example is injection molded from polypropylene. Due to the predetermined breaking points that disconnect the activator from the frame in the event of a pulling force from the spray can, the wall thickness of these breaking points is relatively thin, and results in Figure 4 An acceptable maximum displacement of about 0.19 mm is shown around region 310. When various factors including stress, displacement, reaction force and strain were tested, the actuator exhibited an acceptable range.

Claims

1. An actuator for connecting an injection tool and a fluid delivery device, wherein the fluid delivery device comprises a canister containing a liquid formulation, wherein a top of the canister has a lip, and wherein the fluid delivery device further comprises a stem connected to the canister, The actuator comprises: an activator, wherein the activator is configured to trigger or activate the stem of the fluid delivery device by pressing the activator, and wherein the activator is configured to mount the injection tool; as well as A frame, wherein the frame includes one or more spreaders that press against a lip of the fluid delivery device and pull themselves against the lip, wherein the frame has one or more predetermined breaking points that are configured to break when the activator is pushed downward.

2. The actuator according to claim 1, wherein: The activator has a detent configured to receive the injection tool to facilitate a precise connection between the actuator and the injection tool.

3. The actuator according to claim 1 or 2, wherein: The frame includes at least one second locking mechanism, and the at least one first and at least one second locking mechanisms engage after the activator is pushed downward to hold the activator in the depressed position.

4. The actuator according to any one of claims 1 to 3, wherein: The activator is injection molded as one part.

5. An injection tip configured to deliver a liquid formulation into a plant, the injection tip comprising: a cutting edge at a distal end of the injection tip; an injection tip base at a proximal end of the injection tip; a main post extending from the cutting edge to the injection tip base along a central longitudinal axis of the injection tip; at least two side walls extending from each end of the cutting edge to the injection tip base, wherein the cutting edge, side walls and injection tip base form a wedge-shaped body profile extending along a longitudinal axis; opposing faces extending from the injection tip base and intersecting at the cutting edge; at least two cavities, at least one cavity on each side of said main column, wherein each cavity is configured as an aperture through said opposing faces; a channel extending through the injection tip base along the central longitudinal axis and terminating at a post portion of the main post, wherein the channel is wider than the post portion of the main post; a hole extending from the base of the injection tip upwardly along the passage through the column portion of the main column, Wherein the channel is configured to receive the liquid formulation and to empty the liquid formulation into the cavity via the aperture.

6. The injection tip of claim 5, configured to deliver the liquid formulation at an average flow rate between about 50 ml / min and about 235 ml / min.

7. The injection tip according to claim 5, wherein: The main post includes a shoulder portion proximate the cutting edge and a post portion proximate the base of the injection tip; as well as Each chamber contains: a main region at least partially defined by the sidewalls and further defined by the shoulders of the main posts, wherein the main region has a maximum longitudinal height; as well as A secondary region is defined at least in part by the shoulder and the post portion of the primary post, wherein the secondary region has a maximum longitudinal height that is less than a maximum longitudinal height of the primary region.

8. The injection tip of claim 7, configured to deliver the liquid formulation at an average flow rate of at least about 235 ml / min.

9. An injection tool configured to deliver a liquid formulation into a plant, the injection tool comprising: An injection tip according to any one of claims 5 to 8 connected to a socket, The channel of the injection tip is configured to receive the liquid formulation and to empty the liquid formulation into the cavity via the aperture.

10. The injection tool according to claim 9, wherein: The injection tip is coupled to the socket via a sealing area, wherein the sealing area includes a primary seal.

11. The injection tool according to claim 10, wherein: The primary seal defines a lip oriented perpendicular to the longitudinal axis.

12. The injection tool according to claim 10 or 11, wherein: The sealing area also includes a secondary seal disposed between the primary seal and the socket.

13. The injection tool according to claim 12, wherein: The secondary seal has an outer circumference that is greater than a circumference of the primary seal.

14. The injection tool according to any one of claims 7 to 13, wherein: The receptacle has a single port.

15. The injection tool according to any one of claims 7 to 14, wherein: The socket is a Y-shaped socket.

16. The injection tool according to any one of claims 7 to 14, wherein: The socket is an H-shaped socket.

17. The injection tool according to any one of claims 9 to 16, wherein: The socket is configured to be inserted into an actuator such that the injection tool is fluidly connected with the fluid delivery device through a connection via the actuator.

18. A plant injection system comprising: An actuator according to any one of claims 1 to 4; An injection tool according to any one of claims 9 to 17; and a fluid delivery device, The socket of the injection tool is configured to be insertable into the actuator so that the injection tool is fluidly connected to the fluid delivery device through a connection via the actuator.

19. The plant injection system of claim 18, wherein: The injection tool is configured to be insertable into the actuator perpendicularly relative to the fluid delivery device.

20. The plant injection system of claim 18, wherein: The injection tool is configured to be insertable into the actuator horizontally relative to the fluid delivery device.

21. A plant injection system according to any one of claims 18 to 20, wherein: The fluid delivery device includes a pressurized canister.

22. A plant injection system according to any one of claims 18 to 21, wherein: The fluid delivery device includes a canister having a bag-on-valve insert.

23. The plant injection system of any one of claims 18 to 22, configured to deliver the liquid formulation contained in the fluid delivery device to the vasculature of the plant.

24. A plant injection system according to any one of claims 18 to 23, wherein: The liquid formulations contain one or more active ingredients.

25. A plant injection system according to any one of claims 18 to 24, wherein: The injection means releases the liquid formulation into the active vasculature of the plant part.

26. A method for positioning and mounting a plant injection system according to any one of claims 18 to 25 on a plant part, the method comprising: installing the injection tool into the trunk or stem of the plant part; Setting the injection tool by pressing the top beam; as well as The fluid delivery device is pushed so that a predetermined breaking point of the actuator bridge allows the activator to snap into the frame of the actuator.

27. A method of using an injection tool according to any one of claims 9 to 17 or an injection system according to any one of claims 18 to 25 to dispense a liquid formulation to a plant, the method comprising: penetrating the plant with the injection tool; as well as The liquid formulation is dispensed to the plants by means of the injection tool.

28. The method according to claim 27, wherein: Penetrating the plant includes: The plant is penetrated with the cutting edge, wherein the injection tip is moved along the longitudinal body axis of the injection tool.

29. The method according to claim 27 or 28, wherein: Dispensing the liquid formulation to the plant comprises: delivering the liquid formulation from the inlet of the injection tool to the orifice of the injection tip; and The liquid formulation is delivered from the aperture to the dispensing reservoir.

30. The method according to any one of claims 27 to 29, further comprising: The liquid formulation is retained along the plant tissue of the plant.

31. The method according to any one of claims 27 to 30, wherein: The liquid formulation is dispensed over one or more hours.

32. A method according to any one of claims 27 to 31, wherein The liquid formulation is dispensed continuously.

33. A method of modulating the phenotype of a plant or plants, or treating plants infected with pathogens, or mitigating, controlling and / or eradicating pathogens in plants, or improving abiotic or biotic stress tolerance in plants, The method comprises: installing a plant injection system according to any one of claims 18 to 25 in said plant or plants, and The liquid formulation with active ingredients is applied to modulate the phenotype of the plant, or to treat plants infected with pathogens, or to reduce, control and / or eradicate pathogens in plants, or to improve abiotic or biotic stress tolerance in plants.

34. The method of claim 33, wherein: The pathogen is a viral pathogen, a bacterial pathogen, a fungus, a nematode or an insect pest.

35. The method according to any one of claims 27 to 34, wherein: The liquid formulation is delivered into the vasculature of the plant.

36. A method according to any one of claims 27 to 35, wherein: The plant is a tree crop; or a citrus tree; or a fruit crop; or a vine crop; or a coffee tree, a coconut tree, a pineapple tree, a cocoa tree, a tea tree, a banana tree, a laurel tree, a fig tree, a guava tree, a mango tree, an olive tree, a papaya tree, a cashew tree, a macadamia tree, an almond tree, a natural rubber tree, a date tree, an oil palm tree, an ornamental tree, a forestry tree or a boxwood tree.

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