Microfluidic chip and microfluidic analysis system

Abstract

The application is suitable for the field of medical equipment, and discloses a micro-fluidic chip and a micro-fluidic analysis system. The micro-fluidic chip comprises a non-circular chip main body, and the non-circular chip main body is formed with a sample inlet cavity, a first sample quantitative cavity, a diluent inlet cavity, a diluent quantitative cavity, a mixing cavity, a distribution cavity, a reaction detection cavity, a first overflow cavity and a second overflow cavity. The reaction detection cavity is used for reacting reagents and mixed liquid into a sample. The first overflow cavity is respectively communicated with the diluent quantitative cavity and the distribution cavity, so as to collect the diluent overflowing from the diluent quantitative cavity and collect the mixed liquid overflowing from the distribution cavity. The second overflow cavity is communicated with the first sample quantitative cavity, so as to collect the sample overflowing from the first sample quantitative cavity. The micro-fluidic chip is simple and convenient to use, and can reduce the cost of sample recheck, and reduce the waste of unnecessary samples and consumables.

Description

Technical Field

[0001] This application relates to the field of medical devices, and more particularly to a microfluidic chip and a microfluidic analysis system having the microfluidic chip. Background Technology

[0002] Microfluidic analysis technology integrates the basic operational units of sample analysis, such as sample addition, separation, dilution, reaction, and detection, onto a microfluidic chip with microchannels (tens to hundreds of micrometers in size), automatically completing the entire sample analysis process.

[0003] Traditional microfluidic chips are complete circular sheets with multiple reaction detection chambers distributed around their circumference. Each chamber is used to carry a sample and perform one test. When using this complete circular microfluidic chip for sample testing, it only offers package testing functionality (i.e., testing is performed based on the test items performed by each reaction detection chamber). When medical personnel question the results of a particular test item in the package, or if the results of a particular test item are inaccurate, a retest is required. Using a complete circular microfluidic chip for retesting one, two, or three tests results in most of the reaction detection chambers being redundant. This increases the cost of retesting, wastes the redundant reaction detection chambers, and requires more sample, leading to unnecessary waste of sample, diluent, and reagents.

[0004] To address the aforementioned technical issues, a fan-shaped microfluidic chip has been provided. Multiple fan-shaped microfluidic chips can be assembled into a complete circular microfluidic structure. This fan-shaped microfluidic chip can reduce the waste of unnecessary reaction detection chambers, samples, diluents, and reagents to a certain extent. However, due to its unreasonable structural layout, this fan-shaped microfluidic chip still has the following shortcomings in practical applications: (1) This fan-shaped microfluidic chip does not have a whole blood sample separation function, nor does it have a quantitative sample and diluent function. As a result, the operation of using this fan-shaped microfluidic chip to detect whole blood samples is relatively complicated. It is necessary to first centrifuge the whole blood, take out a certain amount of plasma and add it to the diluent tube, invert and mix it several times, and then take out a certain amount of the mixed solution and add it to the fan-shaped microfluidic chip. (2) The central angle of this fan-shaped microfluidic chip is 120°, and with this layout, it is difficult to further miniaturize it. There are still many reaction detection chambers on it, which will still cause waste of reaction detection chambers, samples, diluents, and reagents. Summary of the Invention

[0005] The first objective of this application is to provide a microfluidic chip that addresses the technical problems of high cost and significant waste of samples and consumables when using microfluidic chips for re-examination in related technologies.

[0006] To achieve the above objectives, the solution provided in this application is: a microfluidic chip, characterized in that: it includes a non-circular chip body, wherein the non-circular chip body is formed with a sample injection chamber, a first sample quantification chamber, a diluent injection chamber, a diluent quantification chamber, a mixing chamber, a dispensing chamber, a reaction detection chamber, a first overflow chamber, and a second overflow chamber;

[0007] The sample inlet chamber is used to store the sample entering the microfluidic chip;

[0008] The first sample quantification chamber is connected to the sample injection chamber for quantifying the sample from the sample injection chamber when the microfluidic chip is centrifuged and rotated.

[0009] The diluent chamber is used to store the diluent that enters the microfluidic chip;

[0010] The diluent metering chamber is connected to the diluent inlet chamber to meter the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged and rotated.

[0011] The mixing chamber is connected to the first sample metering chamber and the diluent metering chamber, respectively, to receive and mix the sample entering from the first sample metering chamber and the diluent entering from the diluent metering chamber when the microfluidic chip is centrifuged and rotated.

[0012] The dispensing chamber is connected to the mixing chamber and the reaction detection chamber respectively, so as to receive the mixture formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged and rotated, and dispense the mixture into the reaction detection chamber;

[0013] The reaction detection chamber is used for the reaction of reagents with the mixture to form a sample;

[0014] The first overflow chamber is connected to the diluent metering chamber and the dispensing chamber, respectively, for collecting the diluent overflowing from the diluent metering chamber and the mixture overflowing from the dispensing chamber;

[0015] The second overflow chamber is connected to the first sample quantification chamber for collecting the sample overflowing from the first sample quantification chamber.

[0016] In one embodiment, the non-circular chip body also forms a sample judgment cavity, which is connected to the second overflow cavity, so as to allow the optical detection component to detect whether there is a sample overflowing from the first sample quantification cavity;

[0017] The distance from the sample judgment cavity to the rotation center axis of the microfluidic chip is the same as the distance from the reaction detection cavity to the rotation center axis of the microfluidic chip.

[0018] In one embodiment, the non-circular chip body also forms a diluent detection cavity, which is connected to the first overflow cavity, for use by the optical detection component to detect whether there is diluent overflowing from the diluent metering cavity;

[0019] The distance from the dilution determination chamber to the rotation center axis of the microfluidic chip is the same as the distance from the reaction detection chamber to the rotation center axis of the microfluidic chip.

[0020] In one embodiment, the distance from the dilution fluid determination chamber to the rotation center axis of the microfluidic chip is greater than the distance from the first overflow chamber to the rotation center axis of the microfluidic chip.

[0021] In one embodiment, the sample determination chamber and the diluent determination chamber are located on opposite sides of the reaction detection chamber pair along the direction of centrifugal rotation of the microfluidic chip.

[0022] In one embodiment, the non-circular chip body also forms a first channel and a second channel. The two ends of the first channel are respectively connected to the distribution cavity and the first overflow cavity, and the two ends of the second channel are respectively connected to the distribution cavity and the reaction detection cavity. The width of the first channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

[0023] In one embodiment, the non-circular chip body has a first plate surface and a second plate surface arranged opposite to each other. The sample injection chamber, the first sample quantification chamber, the diluent injection chamber, the diluent quantification chamber, the mixing chamber, the dispensing chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber are all recessed from the first plate surface toward the second plate surface, and all have a distance between them and the second plate surface.

[0024] The microfluidic chip further includes a sealing membrane, which is attached to the first plate surface to at least cover the sample injection chamber, the first sample quantification chamber, the diluent injection chamber, the diluent quantification chamber, the mixing chamber, the dispensing chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber.

[0025] The sealing membrane has a sample injection hole that extends through the sample inlet chamber at a location corresponding to the sample inlet chamber. The sample injection hole is connected to the sample inlet chamber for injecting the sample into the sample inlet chamber.

[0026] In one embodiment, the sealing membrane has a diluent injection hole penetrating through it at a position corresponding to the diluent inlet chamber. The diluent injection hole communicates with the diluent inlet chamber for injecting diluent into it; or...

[0027] A diluent packet is placed inside the diluent inlet chamber.

[0028] In one embodiment, the non-circular chip body also forms a diluent overflow channel and a sample overflow channel. The diluent overflow channel is connected between the diluent metering chamber and the first overflow chamber, and the sample overflow channel is connected between the first sample metering chamber and the second overflow chamber.

[0029] The sealing membrane is also provided with a first vent, a second vent, and a third vent. The first vent is connected to the diluent overflow channel, the second vent is connected to the sample overflow channel, and the third vent is connected to the mixing chamber.

[0030] In one embodiment, the non-circular chip body also forms a second sample quantification cavity, a sample quantification channel, and a sample drainage capillary, with the two ends of the sample quantification channel respectively connected to the first sample quantification cavity and the second sample quantification cavity;

[0031] The distance from the sample quantification channel to the rotation center axis of the microfluidic chip is greater than the distance from the first sample quantification cavity to the rotation center axis of the microfluidic chip, and less than the distance from the second sample quantification cavity to the rotation center axis of the microfluidic chip.

[0032] The two ends of the sample drainage capillary are respectively connected to the sample quantification channel and the mixing chamber, and the sample drainage capillary has a first bend. The distance from the first bend to the rotation center axis of the microfluidic chip is less than the distance from the first sample quantification chamber to the rotation center axis of the microfluidic chip.

[0033] In one embodiment, the non-circular chip body also forms a mixed liquid drainage capillary and an initial liquid cavity;

[0034] The two ends of the mixture drainage capillary are respectively connected to the mixing chamber and the dispensing chamber, and the mixture drainage capillary has a second bend. The distance from the second bend to the rotation center axis of the microfluidic chip is less than the distance from the mixing chamber to the rotation center axis of the microfluidic chip.

[0035] The initial liquid chamber is connected to the end of the distribution chamber near the end of the mixed liquid drainage capillary, so as to at least collect the initial liquid entering the distribution chamber from the mixed liquid drainage capillary.

[0036] The distance from the initial liquid chamber to the rotation center axis of the microfluidic chip is less than the distance from the reaction detection chamber to the rotation center axis of the microfluidic chip.

[0037] In one implementation, the volume of the initial liquid chamber is smaller than the volume of the reaction detection chamber; and / or,

[0038] The non-circular chip body also forms a second channel and a third channel. The two ends of the second channel are respectively connected to the dispensing cavity and the reaction detection cavity. The two ends of the third channel are respectively connected to the dispensing cavity and the initial liquid cavity. The width of the third channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

[0039] In one embodiment, the non-circular chip body includes a first edge and a second edge, the first edge and the second edge being disposed opposite to each other at a distance, the first edge being disposed at one end of the microfluidic chip near the rotation center axis of the microfluidic chip's centrifugal rotation, and the second edge being disposed at one end of the microfluidic chip away from the rotation center axis of the microfluidic chip's centrifugal rotation;

[0040] The diluent inlet chamber, the diluent metering chamber, the mixing chamber, the dispensing chamber, and the first overflow chamber are arranged sequentially between the first edge and the second edge;

[0041] The injection chamber, the first sample quantification chamber, the mixing chamber, the dispensing chamber, and the second overflow chamber are arranged sequentially between the first edge and the second edge.

[0042] In one implementation, the length of the second edge is greater than the length of the first edge.

[0043] In one implementation, the first edge and the second edge are two concentric arc-shaped edges; or,

[0044] The first edge and the second edge are two parallel straight lines.

[0045] In one embodiment, the non-circular chip body further includes a third edge and a fourth edge;

[0046] The third edge and the fourth edge are spaced apart and opposite to each other. The third edge extends from one end of the first edge to one end of the second edge, and the fourth edge extends from the other end of the first edge to the other end of the second edge.

[0047] The diluent inlet chamber, the diluent metering chamber, one end of the mixing chamber, one end of the dispensing chamber, and the first overflow chamber are arranged sequentially along the third edge;

[0048] The injection chamber, the first sample quantification chamber, and the second overflow chamber are arranged sequentially along the fourth edge.

[0049] In one implementation, the included angle formed by the third edge and the fourth edge is greater than 0° and less than or equal to 90°.

[0050] In one embodiment, the included angle formed by the third edge and the fourth edge is 60°±15°.

[0051] In one implementation, the number of reaction detection cavities formed by the non-circular chip body is less than or equal to six.

[0052] In one implementation, the number of reaction detection cavities formed by the non-circular chip body is two, three, four, or five.

[0053] The second objective of this application is to provide a microfluidic chip comprising a non-circular chip body, wherein the non-circular chip body is formed with a sample inlet chamber, a first sample quantification chamber, a diluent inlet chamber, a diluent quantification chamber, a mixing chamber, a dispensing chamber, a reaction detection chamber, a third overflow chamber, and a fourth overflow chamber.

[0054] The first sample quantification chamber is connected to the sample injection chamber for quantifying the sample from the sample injection chamber when the microfluidic chip is centrifuged and rotated.

[0055] The diluent metering chamber is connected to the diluent inlet chamber to meter the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged and rotated.

[0056] The mixing chamber is connected to the first sample metering chamber and the diluent metering chamber, respectively, to receive and mix the sample entering from the first sample metering chamber and the diluent entering from the diluent metering chamber when the microfluidic chip is centrifuged and rotated.

[0057] The dispensing chamber is connected to the mixing chamber and the reaction detection chamber respectively, so as to receive the mixture formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged and rotated, and dispense the mixture into the reaction detection chamber;

[0058] The reaction detection chamber is used for the reaction of reagents with the mixture to form a sample;

[0059] The third overflow chamber is connected to the diluent metering chamber for collecting the diluent overflowing from the diluent metering chamber;

[0060] The fourth overflow chamber is connected to the first sample quantification chamber and the distribution chamber, respectively, for collecting the sample overflowing from the first sample quantification chamber and collecting the mixture overflowing from the distribution chamber.

[0061] The third objective of this application is to provide a microfluidic analysis system, which includes a turntable, an optical detection assembly, a rotary drive mechanism, and the aforementioned microfluidic chip;

[0062] The turntable has multiple accommodating positions arranged sequentially along the circumference, each of which is used to accommodate one of the microfluidic chips or a counterweight component with the same outer contour and weight as the microfluidic chip.

[0063] The optical detection assembly includes a light emitter and a light receiver. The light emitter is positioned above the turntable to irradiate light toward the sample in the reaction detection chamber.

[0064] The light receiver is located below the turntable and directly below the light emitter, for receiving light emitted by the light emitter that passes through the microfluidic chip;

[0065] The rotary drive mechanism is used to drive the turntable to rotate the microfluidic chip, so as to respectively realize: quantitative analysis of sample and diluent, mixing of sample and diluent, distribution of mixture, and rotating the reaction detection cavity to directly below the photodetector.

[0066] The microfluidic chip and microfluidic analysis system provided in this application achieve sample loading onto the microfluidic chip through the sample injection chamber, and diluent loading onto the microfluidic chip through the diluent injection chamber. The sample is quantified from the sample injection chamber through the first sample quantification chamber, and the diluent is quantified from the diluent injection chamber through the diluent quantification chamber. The sample entering from the first sample quantification chamber and the diluent entering from the diluent quantification chamber are received and mixed through the mixing chamber. The mixture formed by the sample and diluent entering from the mixing chamber is received through the dispensing chamber and dispensed to the reaction detection chamber. Excess diluent and sample are collected and quantified through the first overflow chamber and the second overflow chamber. Thus, after the sample and diluent are added to the microfluidic chip, the rotation of the microfluidic chip automatically completes the quantification, mixing, dispensing, and detection of the sample and diluent. Its operation is simple and the quantification is accurate. Because the sample injection chamber, first sample quantification chamber, diluent injection chamber, diluent quantification chamber, mixing chamber, dispensing chamber, reaction detection chamber, first overflow chamber, and second overflow chamber are all formed within a non-circular chip body—meaning the microfluidic chip is not a perfectly circular chip—it is advantageous to reduce the number of reaction detection chambers and minimize waste of samples, diluents, and reagents during retesting. Furthermore, since the first overflow chamber collects both the diluent overflowing from the diluent quantification chamber and the mixture overflowing from the dispensing chamber, it effectively combines the overflow chambers for diluent quantification and mixture quantification into one, thereby reducing the number of overflow chambers. This simplifies the microfluidic chip's structure and improves its compactness, ultimately facilitating miniaturization and further reducing material costs, ultimately lowering the cost of retesting using this microfluidic chip. Attached Figure Description

[0067] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0068] Figure 1 This is a top view of the microfluidic chip provided in Embodiment 1 of this application;

[0069] Figure 2 This is a schematic diagram of the state after the sample and diluent are added to the microfluidic chip, as provided in Embodiment 1 of this application;

[0070] Figure 3 This is a schematic diagram of the state of the microfluidic chip provided in Embodiment 1 of this application after the first centrifugation and rotation to complete the quantification of diluent and sample;

[0071] Figure 4This is a schematic diagram of the state of sample and diluent being drained by capillary action after the microfluidic chip provided in Embodiment 1 of this application stops its first centrifugal rotation;

[0072] Figure 5 This is a schematic diagram of the state of the microfluidic chip provided in Embodiment 1 of this application after a second centrifugal rotation to complete the mixing of the diluent and the sample;

[0073] Figure 6 This is a schematic diagram of the state of the mixture being guided by the capillary after the microfluidic chip provided in Embodiment 1 of this application stops its second centrifugal rotation;

[0074] Figure 7 This is a schematic diagram of the state of the microfluidic chip provided in Embodiment 1 of this application after the mixture is distributed by the third centrifugal rotation;

[0075] Figure 8 This is a schematic diagram of the front view structure of the microfluidic chip provided in Embodiment 1 of this application;

[0076] Figure 9 This is a schematic diagram of the distribution of multiple microfluidic chips on a turntable provided in Embodiment 1 of this application;

[0077] Figure 10 This is a schematic diagram showing the positions of the microfluidic chip and the optical detection component provided in Embodiment 1 of this application;

[0078] Figure 11 This is a schematic diagram of the composition of the microfluidic analysis system provided in Embodiment 1 of this application;

[0079] Figure 12 This is a top view of the microfluidic chip provided in Embodiment 2 of this application;

[0080] Figure 13 This is a top view of the microfluidic chip provided in Embodiment 3 of this application.

[0081] Reference numerals: 100, Microfluidic chip; 110, Non-circular chip body; 111, Sample injection chamber; 112, First sample quantification chamber; 113, Diluent injection chamber; 114, Diluent quantification chamber; 115, Mixing chamber; 116, Dispensing chamber; 117, Reaction detection chamber; 118, First overflow chamber; 119, Second overflow chamber; 101, Sample judgment chamber; 102, Diluent judgment chamber; 103, Diluent overflow channel; 104, Sample overflow channel; 105, Second sample quantification chamber; 106, Sample quantification conduit; 107, Sample drainage capillary; 1071, First bend; 108, Diluent drainage capillary; 1081, Third bend; 109, Mixture drainage capillary; 1091, First... Two bends; 1001, Initial liquid chamber; 1002, First channel; 1003, Second channel; 1004, Third channel; 1005, First edge; 1006, Second edge; 1007, Third edge; 1008, Fourth edge; 1009, First plate surface; 1010, Second plate surface; 1011, Third overflow chamber; 1012, Fourth overflow chamber; 120, Sealing membrane; 121, Sample injection hole; 122, Diluent injection hole; 123, First vent hole; 124, Second vent hole; 125, Third vent hole; 200, Turntable; 300, Optical detection assembly; 310, Light emitter; 320, Light receiver; 400, Rotation drive mechanism; 500, Controller; MN, Rotation center axis. Detailed Implementation

[0082] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0083] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of this application are only used to explain the relative positional relationship and movement of each component in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0084] It should also be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly on the other component or may be connected to an intermediary component. When a component is referred to as being "connected to" another component, it can be directly connected to the other component or indirectly connected to the other component through an intermediary component.

[0085] Furthermore, the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. When the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed in this application.

[0086] Example 1:

[0087] like Figure 1 , Figure 8 and Figure 9 As shown, the microfluidic chip 100 provided in Embodiment 1 of this application includes a non-circular chip body 110. Specifically, the non-circular chip body 110 is a chip structure with a non-circular circumference, meaning its outer edge is not circular. The microfluidic chip 100 has a non-circular overall structure, meaning it is not perfectly circular. Compared to perfectly circular chips, the material cost of the microfluidic chip 100 can be reduced. When used for detecting a small number of items (e.g., retesting some items), it can reduce costs and the consumption of samples, diluents, and reagents. When used for detecting a large number of items, two or more microfluidic chips 100 can be combined to meet the detection needs of different numbers of items, offering high flexibility.

[0088] Reference Figure 1 , Figure 2 and Figure 8As shown, in one embodiment, the non-circular chip body 110 has a sample inlet chamber 111, a first sample quantification chamber 112, a diluent inlet chamber 113, a diluent quantification chamber 114, a mixing chamber 115, a dispensing chamber 116, and a reaction detection chamber 117. The sample inlet chamber 111 stores the sample entering the microfluidic chip 100; the first sample quantification chamber 112 is connected to the sample inlet chamber 111 for quantifying the sample from the sample inlet chamber 111 when the microfluidic chip 100 is centrifuged; the diluent inlet chamber 113 stores the diluent entering the microfluidic chip 100; the diluent quantification chamber 114 is connected to the diluent inlet chamber 113 for quantifying the diluent from the diluent inlet chamber 113 when the microfluidic chip 100 is centrifuged; the mixing chamber 115 is connected to the first sample quantification chamber 112, the diluent inlet chamber 113, the diluent inlet chamber 114, and the mixing chamber 115. The liquid metering chamber 114 is connected to receive and mix the sample entering from the first sample metering chamber 112 and the diluent entering from the diluent metering chamber 114 when the microfluidic chip 100 is centrifuged and rotated. The dispensing chamber 116 is connected to the mixing chamber 115 and the reaction detection chamber 117, respectively, to receive the mixture formed by mixing the sample and the diluent from the mixing chamber 115 when the microfluidic chip 100 is centrifuged and rotated, and to dispense the mixture to the reaction detection chamber 117. The reaction detection chamber 117 is used for the reagent to react with the mixture to form a sample. In this embodiment, after the sample and diluent are added to the microfluidic chip 100, the quantification, mixing, dispensing and detection of the sample and diluent can be automatically completed by the rotation of the microfluidic chip 100. The operation is simple and convenient, and the quantification is accurate. Since the sample injection chamber 111, the first sample quantification chamber 112, the diluent injection chamber 113, the diluent quantification chamber 114, the mixing chamber 115, the dispensing chamber 116, and the reaction detection chamber 117 are all formed on the non-circular chip body 110, it is beneficial to reduce the number of reaction detection chambers 117 on the microfluidic chip 100, and to reduce the waste of samples, diluents, and reagents during retesting.

[0089] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a first overflow cavity 118, which is connected to the diluent metering cavity 114 to collect the diluent overflowing from the diluent metering cavity 114. The first overflow cavity 118 can be used to collect excess diluent after metering, which helps to ensure that there is enough diluent and prevents excessive diluent, thus effectively ensuring the accuracy of diluent metering.

[0090] Reference Figure 1 , Figure 3 and Figure 7As shown, in one embodiment, the first overflow chamber 118 is also connected to the dispensing chamber 116 for collecting the mixture overflowing from the dispensing chamber 116. In this embodiment, the first overflow chamber 118 is used to collect both the diluent overflowing from the diluent metering chamber 114 and the mixture overflowing from the dispensing chamber 116. This is equivalent to combining the overflow chamber for metering the diluent and the overflow chamber for the mixture into one, thereby reducing the number of overflow chambers, simplifying the structure of the microfluidic chip 100 and improving its compactness. Ultimately, this facilitates the miniaturization design of the microfluidic chip 100, further reducing the material cost of the microfluidic chip 100, and consequently lowering the cost of using the microfluidic chip 100 for testing.

[0091] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a second overflow cavity 119, which is connected to the first sample quantification cavity 112 to collect the sample overflowing from the first sample quantification cavity 112. The second overflow cavity 119 is mainly used to collect excess sample after quantification, which helps to ensure sufficient sample and prevents excessive sample, effectively ensuring the accuracy of sample quantification.

[0092] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a sample determination cavity 101, which is connected to the second overflow cavity 119 for the optical detection component 300 to detect whether there is any sample overflowing from the first sample quantification cavity 112. When the optical detection component 300 detects a sample in the sample determination cavity 101, it indicates that the first sample quantification cavity 112 is full, thus determining that the sample quantity is sufficient; otherwise, it determines that the sample quantity is insufficient. The sample determination cavity 101 helps to further ensure the accuracy and reliability of sample quantification.

[0093] Reference Figure 1 , Figure 3 and Figure 10As shown, in one embodiment, the distance from the sample judgment cavity 101 to the rotation center axis MN of the microfluidic chip 100 is the same as the distance from the reaction detection cavity 117 to the rotation center axis MN of the microfluidic chip 100. In this embodiment, the fact that the distance from the sample judgment cavity 101 to the rotation center axis MN of the microfluidic chip 100 is the same as the distance from the reaction detection cavity 117 to the rotation center axis MN of the microfluidic chip 100 allows the sample judgment cavity 101 and the reaction detection cavity 117 to sequentially pass through the same position when the microfluidic chip 100 rotates. This facilitates the sharing of the optical detection component 300 for determining the presence or absence of a sample in the sample judgment cavity 101 and detecting the sample in the reaction detection cavity 117, thereby simplifying the structure of the microfluidic system. Of course, in specific applications, as an alternative implementation, the determination of the presence or absence of a sample in the sample determination cavity 101 can also be performed by a separate sensor, without sharing the optical detection component 300 with the reaction detection cavity 117. In this way, the distance from the sample determination cavity 101 to the rotation center axis MN of the microfluidic chip 100 can be different from the distance from the reaction detection cavity 117 to the rotation center axis MN of the microfluidic chip 100.

[0094] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a diluent detection chamber 102, which is connected to the first overflow chamber 118 for the optical detection component 300 to detect whether there is diluent overflowing from the diluent metering chamber 114. When the optical detection component 300 detects that there is diluent in the diluent detection chamber 102, it indicates that the diluent metering chamber 114 is full, thus determining that the amount of diluent is sufficient; otherwise, it determines that the amount of diluent is insufficient. The setting of the diluent detection chamber 102 can help to further ensure the accuracy and reliability of diluent metering.

[0095] Reference Figure 1 , Figure 3 and Figure 10As shown, in one embodiment, the distance from the dilution determination chamber 102 to the rotation center axis MN of the microfluidic chip 100 is the same as the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100. In this embodiment, the fact that the distance from the dilution determination chamber 102 to the rotation center axis MN of the microfluidic chip 100 is the same as the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100 allows the dilution determination chamber 102 and the reaction detection chamber 117 to pass through the same position sequentially when the microfluidic chip 100 rotates. This facilitates the sharing of the optical detection component 300 for determining the presence or absence of dilution in the dilution determination chamber 102 and detecting the sample in the reaction detection chamber 117, thereby simplifying the structure of the microfluidic system. Of course, in specific applications, as an alternative implementation, the determination of the presence or absence of diluent in the diluent determination chamber 102 can also be performed by a separate sensor, without sharing the optical detection component 300 with the reaction detection chamber 117. In this way, the distance from the diluent determination chamber 102 to the rotation center axis MN of the microfluidic chip 100 can be different from the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100.

[0096] In one implementation, the diluent determination chamber 102, the reaction detection chamber 117, and the sample determination chamber 101 are sequentially distributed along the same arc trajectory. This allows the diluent determination chamber 102, the reaction detection chamber 117, and the sample determination chamber 101 to pass through the same position sequentially when the microfluidic chip 100 rotates. This facilitates the use of the optical detection component 300 to determine the presence or absence of diluent in the diluent determination chamber 102, the presence or absence of sample in the sample determination chamber 101, and the detection of the sample in the reaction detection chamber 117.

[0097] Reference Figure 1 , Figure 2 and Figure 10 As shown, in one embodiment, the distance from the diluent determination chamber 102 to the rotation center axis MN of the microfluidic chip 100 is greater than the distance from the first overflow chamber 118 to the rotation center axis MN of the microfluidic chip 100. This ensures that excess diluent will preferentially enter the diluent determination chamber 102 under centrifugal action, and will only enter the first overflow chamber 118 after the diluent determination chamber 102 is full.

[0098] Reference Figure 1 , Figure 2 and Figure 10As shown, in one embodiment, the distance from the sample judgment chamber 101 to the rotation center axis MN of the microfluidic chip 100 is greater than the distance from the second overflow chamber 119 to the rotation center axis MN of the microfluidic chip 100. This helps to ensure that excess sample will preferentially enter the sample judgment chamber 101 under centrifugation, and will only enter the second overflow chamber 119 after the diluent judgment chamber 102 is full.

[0099] Reference Figure 1 , Figure 2 and Figure 3 As shown, in one embodiment, the sample judgment cavity 101 and the diluent judgment cavity 102 are located on both sides of the reaction detection cavity 117 pair along the direction of centrifugal rotation of the microfluidic chip 100. That is, the reaction detection cavity 117 is located between the sample judgment cavity 101 and the diluent along the circumference of the microfluidic chip 100. With this arrangement, the sample-related cavities and the diluent-related cavities can be concentrated on both sides of the circumference of the microfluidic chip 100, thereby improving the structural compactness of the microfluidic chip 100.

[0100] Reference Figure 1 , Figure 3 , Figure 4 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a first channel 1002 and a second channel 1003. The two ends of the first channel 1002 are respectively connected to the distribution cavity 116 and the first overflow cavity 118, and the two ends of the second channel 1003 are respectively connected to the distribution cavity 116 and the reaction detection cavity 117. The width L1 of the first channel 1002 in the centrifugal rotation direction of the microfluidic chip 100 is equal to the width L2 of the second channel 1003 in the centrifugal rotation direction of the microfluidic chip 100. In this embodiment, the first channel 1002 and the second channel 1003 are designed to have the same width without distinction, which helps to reduce the manufacturing difficulty of the microfluidic chip 100.

[0101] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a diluent overflow channel 103, which connects the diluent metering chamber 114 and the first overflow chamber 118. The diluent overflow channel 103 is also connected to the diluent judgment chamber 102. The diluent overflow channel 103 is mainly used to guide the diluent overflowing from the diluent metering chamber 114 to the diluent judgment chamber 102 and the first overflow chamber 118.

[0102] In one embodiment, one end of the diluent overflow channel 103 is connected to the end of the diluent metering chamber 114 near the rotation center axis MN of the microfluidic chip 100. In this way, the diluent will enter the diluent overflow channel 103 under centrifugal force after the diluent metering chamber 114 is filled.

[0103] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a sample overflow channel 104, which connects the first sample quantification chamber 112 and the second overflow chamber 119. The sample overflow channel 104 is also connected to the sample judgment chamber 101. The sample overflow channel 104 is mainly used to guide the sample overflowing from the first sample quantification chamber 112 to the sample judgment chamber 101 and the second overflow chamber 119.

[0104] In one implementation, one end of the sample overflow channel 104 is connected to the end of the first sample quantitative cavity 112 near the rotation center axis MN of the microfluidic chip 100. In this way, the sample will enter the sample overflow channel 104 under centrifugal force after the first sample quantitative cavity 112 is filled.

[0105] Reference Figure 1 , Figure 3 , Figure 8 and Figure 10 As shown, in one embodiment, the non-circular chip body 110 also forms a second sample quantification chamber 105 and a sample quantification channel 106. The two ends of the sample quantification channel 106 are respectively connected to the first sample quantification chamber 112 and the second sample quantification chamber 105. The distance from the sample quantification channel 106 to the rotation center axis MN of the microfluidic chip 100 is greater than the distance from the first sample quantification chamber 112 to the rotation center axis MN of the microfluidic chip 100, and less than the distance from the second sample quantification chamber 105 to the rotation center axis MN of the microfluidic chip 100. The second sample quantification chamber 105 is mainly used to meet the requirement of centrifugation and stratification of some samples before detection. For example, when the sample is a whole blood sample, after the sample is centrifuged and quantified, the plasma will be concentrated in the first sample quantification chamber 112, and the red blood cells will be concentrated in the second sample quantification chamber 105.

[0106] Reference Figure 3 , Figure 4 and Figure 8As shown, in one embodiment, the non-circular chip body 110 also has a sample drainage capillary 107, with its two ends connected to the sample quantification channel 106 and the mixing chamber 115, respectively. The sample drainage capillary 107 is mainly used to ensure that the sample quantification and the mixing of the sample into the mixing chamber 115 can be carried out separately during the two centrifugal rotations of the microfluidic chip 100, thereby helping to ensure the accuracy of sample quantification.

[0107] Reference Figure 3 , Figure 4 , Figure 8 and Figure 10 As shown, in one embodiment, the sample drainage capillary 107 has a first bend 1071. The distance from the first bend 1071 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the first sample quantification chamber 112 to the rotation center axis MN of the microfluidic chip 100. In this embodiment, the distance from the first bend 1071 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the first sample quantification chamber 112 to the rotation center axis MN of the microfluidic chip 100, which helps to ensure that the sample does not enter the mixing chamber 115 from the sample drainage capillary 107 during the centrifugal rotation stage of quantifying the sample; during the sample quantification completion and stopping stage, the sample in the sample drainage capillary 107 fills the sample drainage capillary 107 under capillary action; during the centrifugal rotation stage of mixing the sample with the diluent, the sample in the sample drainage capillary 107 flows into the mixing chamber 115 under siphon action.

[0108] In one embodiment, one end of the sample drainage capillary 107 is connected to the end of the sample quantitative pipeline 106 near the first sample quantitative chamber 112, and the other end of the sample drainage capillary 107 is connected to the end of the mixing chamber 115 near the rotation center axis MN of the microfluidic chip 100.

[0109] In one embodiment, the first bending portion 1071 is arranged in an arc-shaped bend.

[0110] Reference Figure 3 , Figure 4 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a diluent drainage capillary 108. The two ends of the diluent drainage capillary 108 are respectively connected to the diluent metering chamber 114 and the mixing chamber 115. The diluent drainage capillary 108 is mainly used to ensure the metering of the diluent and that the mixing of the diluent into the mixing chamber 115 can be carried out in two centrifugal rotations of the microfluidic chip 100, thereby helping to ensure the accuracy of the diluent metering.

[0111] Reference Figure 3 , Figure 4 , Figure 8and Figure 10 As shown, in one embodiment, the diluent draining capillary 108 has a third bend 1081. The distance from the third bend 1081 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the diluent metering chamber 114 to the rotation center axis MN of the microfluidic chip 100. In this embodiment, the distance from the third bend 1081 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the diluent metering chamber 114 to the rotation center axis MN of the microfluidic chip 100, which helps to ensure that during the centrifugal rotation stage of the metered diluent, the diluent will not enter the mixing chamber 115 from the diluent draining capillary 108; during the metered diluent completion and stopping stage, the diluent in the diluent draining capillary 108 fills the diluent draining capillary 108 under capillary action; during the mixing centrifugal rotation stage, the diluent in the diluent draining capillary 108 flows into the mixing chamber 115 under siphon action. Specifically, the quantification of the diluent and the quantification of the sample are performed in the same centrifugal rotation stage of the microfluidic chip 100, and the diluent and the sample enter the mixing chamber 115 in the same centrifugal rotation stage of the microfluidic chip 100.

[0112] In one embodiment, one end of the diluent drainage capillary 108 is connected to the end of the diluent metering chamber 114 away from the rotation center axis MN of the microfluidic chip 100, and the other end of the diluent drainage capillary 108 is connected to the end of the mixing chamber 115 near the rotation center axis MN of the microfluidic chip 100.

[0113] In one embodiment, the third bending portion 1081 is configured in an arc-shaped bend.

[0114] Reference Figures 5 to 8 As shown, in one embodiment, the non-circular chip body 110 also forms a mixing liquid drainage capillary 109 and an initial liquid chamber 1001. The two ends of the mixing liquid drainage capillary 109 are respectively connected to the mixing chamber 115 and the distribution chamber 116. The initial liquid chamber 1001 is connected to the end of the distribution chamber 116 near the mixing liquid drainage capillary 109, at least for collecting the initial liquid entering the distribution chamber 116 from the mixing liquid drainage capillary 109. The mixing liquid drainage capillary 109 is mainly used to ensure that the mixing of the diluent and the sample, and the distribution of the mixed liquid into the distribution chamber 116, can be performed separately during the two centrifugal rotations of the microfluidic chip 100. This facilitates ensuring that the diluent and the sample are fully mixed before entering the distribution chamber 116, thereby ensuring the accuracy of sample detection in the reaction detection chamber 117. The initial liquid chamber 1001 is used to collect unmixed liquid from the mixing liquid drainage capillary 109, which helps ensure the accuracy of sample detection in the reaction detection chamber 117.

[0115] Reference Figure 7 , Figure 8 and Figure 10 As shown, in one embodiment, the distance from the initial liquid chamber 1001 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100. In this embodiment, the distance from the initial liquid chamber 1001 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the reaction detection chamber 117 to the rotation center axis MN of the microfluidic chip 100. This helps to ensure that when the microfluidic chip 100 is centrifuged, the liquid entering the distribution chamber 116 from the mixed liquid drainage capillary 109 will preferentially fill the initial liquid chamber 1001, so that the unmixed liquid can preferentially enter the initial liquid chamber 1001.

[0116] In one implementation, the reaction detection chamber 117 is positioned circumferentially between the initial liquid chamber 1001 and the first overflow chamber 118 along the centrifugal rotation of the microfluidic chip 100. This allows the initial liquid chamber 1001 and the first overflow chamber 118 to be positioned close to the two ends of the distribution chamber 116, which helps to ensure that the liquid entering the distribution chamber 116 from the mixed liquid guide capillary 109 first fills the initial liquid chamber 1001, then fills the reaction detection chamber 117, and finally enters the first overflow chamber 118.

[0117] Reference Figures 5 to 8 As shown, in one embodiment, the mixing liquid drainage capillary 109 has a second bend 1091. The distance from the second bend 1091 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the mixing chamber 115 to the rotation center axis MN of the microfluidic chip 100. In this embodiment, the distance from the second bend 1091 to the rotation center axis MN of the microfluidic chip 100 is less than the distance from the mixing chamber 115 to the rotation center axis MN of the microfluidic chip 100, which helps to ensure that during the centrifugal rotation stage of mixing the diluent and the sample, the sample and diluent will not enter the distribution chamber 116 from the mixing liquid drainage capillary 109; during the mixing completion and stopping stage, the mixture in the mixing liquid drainage capillary 109 fills the mixing liquid drainage capillary 109 under capillary action; during the centrifugal rotation stage of distributing the mixture, the mixture in the mixing liquid drainage capillary 109 flows into the distribution chamber 116 under siphon action.

[0118] In one embodiment, one end of the mixing liquid drainage capillary 109 is connected to the end of the mixing chamber 115 away from the rotation center axis MN of the microfluidic chip 100, and the other end of the mixing liquid drainage capillary 109 is connected to the end of the dispensing chamber 116 near the rotation center axis MN of the microfluidic chip 100.

[0119] In one embodiment, the second bending portion 1091 is configured in an arc-shaped bend.

[0120] Reference Figure 5 , Figure 6and Figure 7 As shown, in one embodiment, the volume of the initial liquid chamber 1001 is smaller than the volume of the reaction detection chamber 117. Since the initial liquid chamber 1001 is mainly used to collect a small amount of unmixed liquid in the mixed liquid drainage capillary 109, designing the initial liquid chamber 1001 to be smaller than the reaction detection chamber 117 can help avoid excessive mixed liquid entering the initial liquid chamber 1001 after mixing in the mixing chamber 115, which would waste the sample and diluent, and ensure that the subsequent reaction detection chamber 117 can collect a sufficient amount of mixed liquid.

[0121] Reference Figure 3 , Figure 4 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 also forms a second channel 1003 and a third channel 1004. The two ends of the second channel 1003 are connected to the dispensing cavity 116 and the reaction detection cavity 117, respectively. The two ends of the third channel 1004 are connected to the dispensing cavity 116 and the initial liquid cavity 1001, respectively. The width L3 of the third channel 1004 in the centrifugal rotation direction of the microfluidic chip 100 is equal to the width L2 of the second channel 1003 in the centrifugal rotation direction of the microfluidic chip 100. The width L3 of the third channel 1004 in the centrifugal rotation direction of the microfluidic chip 100 is the circumferential width of the third channel 1004. The width L2 of the second channel 1003 in the centrifugal rotation direction of the microfluidic chip 100 is the circumferential width of the second channel 1003. In this embodiment, the second channel 1003 and the third channel 1004 are designed with equal widths without distinction, which helps to reduce the manufacturing difficulty of the microfluidic chip 100.

[0122] In one embodiment, the reaction detection chamber 117 is filled with reagents. After the mixture enters the reaction detection chamber 117, it reacts with the reagents to form a sample.

[0123] In one implementation, the reagent loaded in the reaction detection chamber 117 is a lyophilized bulb reagent. Lyophilized bulb reagents are reagents prepared using the lyophilization method. The small volume of lyophilized bulb reagents helps to increase the shelf life of the reagent. Using smaller volume lyophilized bulb reagents allows for a larger sample load in the same size reaction detection chamber 117, thereby improving detection sensitivity and efficiency.

[0124] Reference Figure 1 , Figure 3 and Figure 8As shown, in one embodiment, the non-circular chip body 110 includes a first edge 1005 and a second edge 1006. The first edge 1005 and the second edge 1006 are spaced apart and opposite to each other. The first edge 1005 is located at one end of the microfluidic chip 100 near the rotation center axis MN of the microfluidic chip 100, and the second edge 1006 is located at one end of the microfluidic chip 100 away from the rotation center axis MN of the microfluidic chip 100. A diluent inlet chamber 113, a diluent metering chamber 114, a mixing chamber 115, a dispensing chamber 116, and a first overflow chamber 118 are sequentially arranged between the first edge 1005 and the second edge 1006; a sample inlet chamber 111, a first sample metering chamber 112, a mixing chamber 115, a dispensing chamber 116, and a second overflow chamber 119 are sequentially arranged between the first edge 1005 and the second edge 1006. Specifically, the first edge 1005 is the inner edge of the non-circular chip body 110, and the second edge 1006 is the outer edge of the non-circular chip body 110. The diluent inlet chamber 113, the diluent metering chamber 114, the mixing chamber 115, the dispensing chamber 116, and the first overflow chamber 118 are arranged sequentially from the inner edge to the outer edge. The sample inlet chamber 111, the first sample metering chamber 112, the mixing chamber 115, the dispensing chamber 116, and the second overflow chamber 119 are also arranged sequentially from the inner edge to the outer edge. This arrangement helps to ensure the stability of the microfluidic chip. When the sample 100 is centrifuged, the sample in the injection chamber 111 can enter the first sample quantification chamber 112 and the second overflow chamber 119 under centrifugation. The sample in the diluent chamber 113 can enter the diluent quantification chamber 114 and the first overflow chamber 118 under centrifugation. The sample in the first sample quantification chamber 112 can enter the mixing chamber 115 under centrifugation. The diluent in the diluent quantification chamber 114 can enter the mixing chamber 115 under centrifugation. The mixture in the mixing chamber 115 enters the distribution chamber 116 under centrifugation.

[0125] In one embodiment, the second sample quantitative cavity 105 is located on the side of the first sample quantitative cavity 112 facing the second edge 1006. That is, the second sample quantitative cavity 105 is located between the first sample quantitative cavity 112 and the second edge 1006 along the direction from the first edge 1005 to the second edge 1006. This facilitates ensuring that the larger part of the sample can preferentially enter the second sample quantitative cavity 105 under centrifugation, thereby facilitating the centrifugation stratification of the sample. For example, red blood cells in a whole blood sample enter the second sample quantitative cavity 105 under centrifugation, while plasma in a whole blood sample enters the first sample quantitative cavity 112 under centrifugation.

[0126] In one embodiment, the reaction detection chamber 117 is located on the side of the distribution chamber 116 facing the second edge 1006, that is, the reaction detection chamber 117 is located between the distribution chamber 116 and the second edge 1006 along the direction from the first edge 1005 toward the second edge 1006. This facilitates ensuring that the mixture in the distribution chamber 116 can enter the reaction detection chamber 117 under centrifugal action.

[0127] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the non-circular chip body 110 further includes a third edge 1007 and a fourth edge 1008; the third edge 1007 and the fourth edge 1008 are arranged opposite each other at intervals, the third edge 1007 extends from one end of the first edge 1005 to one end of the second edge 1006, and the fourth edge 1008 extends from the other end of the first edge 1005 to the other end of the second edge 1006; the diluent inlet chamber 113, the diluent metering chamber 114, one end of the mixing chamber 115, one end of the dispensing chamber 116, and the first overflow chamber 118 are arranged sequentially along the third edge 1007; the sample inlet chamber 111, the first sample metering chamber 112, and the second overflow chamber 119 are arranged sequentially along the fourth edge 1008. The third edge 1007 and the fourth edge 1008 are the two circumferential edges of the non-circular chip body 110. In this embodiment, the sample-related cavities are arranged along one circumferential edge of the non-circular chip body 110, and the diluent-related cavities are arranged along the other circumferential edge of the non-circular chip body 110, which helps to optimize the layout of each cavity and improve the structural compactness of the microfluidic chip 100.

[0128] In one implementation, the length of the second edge 1006 is greater than the length of the first edge 1005, meaning the outer edge of the microfluidic chip 100 is longer than its inner edge. The third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a gradually decreasing spacing. This arrangement allows for a closer arrangement of adjacent microfluidic chips 100 when multiple microfluidic chips 100 are combined along the circumferential direction. Of course, in specific applications, as an alternative implementation, the length of the second edge 1006 can also be equal to the length of the first edge 1005.

[0129] In one embodiment, the first edge 1005 and the second edge 1006 are two concentric arc-shaped edges. In this embodiment, the microfluidic chip 100 has a fan-shaped structure, the first edge 1005 and the second edge 1006 are both arc-shaped and concentric, and the third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a gradually decreasing spacing.

[0130] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the included angle A formed by the third edge 1007 and the fourth edge 1008 is greater than 0° and less than or equal to 90°, that is, the central angle A of the non-circular chip body 110 is between 0° and 90°. By adopting the above-described layout of the cavities on the non-circular chip body 110, the central angle of the non-circular chip body 110 can be designed to be less than or equal to 90°, thereby making the volume of the non-circular chip body 110 relatively small, which helps to reduce the cost of re-inspection using this microfluidic chip 100 and unnecessary waste.

[0131] In one implementation, the included angle A formed by the third edge 1007 and the fourth edge 1008 is 60°±15°, that is, the central angle A of the non-circular chip body 110 is between 60°±15°.

[0132] In one implementation, the included angle A formed by the third edge 1007 and the fourth edge 1008 is 60°, that is, the central angle A of the non-circular chip body 110 is 60°. In this way, six non-circular chip bodies 110 can be combined to form a complete circular chip structure.

[0133] In one implementation, the number of reaction detection cavities 117 formed by the non-circular chip body 110 is less than or equal to six. This makes the volume of a single microfluidic chip 100 relatively small and the number of reaction detection cavities 117 on a single microfluidic chip 100 relatively small. When a single microfluidic chip 100 is used for retesting, the cost of the microfluidic chip 100 can be reduced, as well as the waste of samples, diluents and reagents can be reduced.

[0134] In one implementation, the non-circular chip body 110 forms three reaction detection cavities 117. This effectively meets the requirements for retesting using a single microfluidic chip 100 and significantly avoids waste of samples, diluents, and reagents. Of course, in specific applications, the number of reaction detection cavities 117 formed by the non-circular chip body 110 can also be two, three, four, or five, etc.

[0135] Reference Figure 1 , Figure 3 and Figure 8As shown, in one embodiment, the non-circular chip body 110 has a first plate surface 1009 and a second plate surface 1010 arranged opposite to each other. The sample injection chamber 111, the first sample quantitative chamber 112, the diluent injection chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the dispensing chamber 116, the reaction detection chamber 117, the first overflow chamber 118 and the second overflow chamber 119 are all recessed from the first plate surface 1009 toward the second plate surface 1010, and all have a distance from the second plate surface 1010. That is, the sample injection chamber 111, the first sample quantitative chamber 112, the diluent injection chamber 113, the diluent quantitative chamber 114, the mixing chamber 115, the dispensing chamber 116, the reaction detection chamber 117, the first overflow chamber 118 and the second overflow chamber 119 are not arranged to penetrate along the thickness direction of the non-circular chip body 110. That is, these cavities are all concave cavity structures similar to blind holes.

[0136] Reference Figure 1 , Figure 3 and Figure 8 As shown, in one embodiment, the microfluidic chip 100 further includes a sealing film 120, which is attached to the first plate surface 1009 to at least cover the sample injection chamber 111, the first sample quantification chamber 112, the diluent injection chamber 113, the diluent quantification chamber 114, the mixing chamber 115, the dispensing chamber 116, the reaction detection chamber 117, the first overflow chamber 118, and the second overflow chamber 119. The sealing film 120 is mainly used to seal and protect the sample injection chamber 111, the first sample quantification chamber 112, the diluent injection chamber 113, the diluent quantification chamber 114, the mixing chamber 115, the dispensing chamber 116, the reaction detection chamber 117, the first overflow chamber 118, and the second overflow chamber 119.

[0137] In one embodiment, the second sample quantitative cavity 105 is also recessed from the first plate surface 1009 toward the second plate surface 1010, and there is a gap between it and the second plate surface 1010. That is, the second sample quantitative cavity 105 is not disposed through the thickness direction of the non-circular chip body 110, that is, the second sample quantitative cavity 105 is a concave cavity structure similar to a blind hole. The sealing film 120 also covers the second sample quantitative cavity 105.

[0138] Reference Figure 1 , Figure 2 and Figure 8 As shown, in one embodiment, the sealing membrane 120 has a sample injection hole 121 penetrating through it at a position corresponding to the sample injection chamber 111. The sample injection hole 121 communicates with the sample injection chamber 111 for injecting samples into the sample injection chamber 111. The sample to be tested can be injected into the sample injection chamber 111 through the sample injection hole 121 to achieve sample loading. Of course, the sample injection hole 121 can also be temporarily formed after the sample is injected into the sample injection chamber 111.

[0139] Reference Figure 1 , Figure 2 and Figure 8 As shown, in one embodiment, the sealing membrane 120 has a diluent injection hole 122 penetrating through it at a position corresponding to the diluent inlet chamber 113. The diluent injection hole 122 communicates with the diluent inlet chamber 113 for injecting diluent into the diluent inlet chamber 113. In this embodiment, the diluent is injected into the diluent inlet chamber 113 through the diluent injection hole 122. Of course, in specific applications, as an alternative embodiment, a diluent pack can also be placed in the diluent inlet chamber 113. When the diluent is needed, the diluent in the diluent pack can be made to flow into the diluent inlet chamber 113 by pressing or puncturing.

[0140] Reference Figure 1 , Figure 2 and Figure 8 As shown, in one embodiment, the sealing membrane 120 is further provided with a first vent 123, which is connected to the diluent overflow channel 103. Since the diluent overflow channel 103 is connected to the diluent metering chamber 114, the first overflow chamber 118, and the diluent judgment chamber 102 respectively, and the first overflow chamber 118 is connected to the distribution chamber 116, the gas in the diluent metering chamber 114 and the gas in the diluent judgment chamber 102 can be discharged through the first vent 123 when metering the diluent; and the gas in the distribution chamber 116 and the reaction detection chamber 117 can also be discharged through the first vent 123 when distributing the mixture. In this embodiment, the diluent metering chamber 114 and the distribution chamber 116 share a vent, which helps to reduce the number of vents and thus simplifies the structure of the microfluidic chip 100.

[0141] Reference Figure 1 , Figure 2 and Figure 8 As shown, in one embodiment, the sealing membrane 120 is further provided with a second vent 124, which is connected to the sample overflow channel 104. Since the sample overflow channel 104 is connected to the first sample quantification chamber 112, the second overflow chamber 119, and the sample judgment chamber 101 respectively, and the first sample quantification chamber 112 is connected to the second sample quantification chamber 105, the gas in the first sample quantification chamber 112, the gas in the second sample quantification chamber 105, and the gas in the sample judgment chamber 101 can be discharged from the second vent 124 during sample quantification.

[0142] Reference Figure 1 , Figure 5 and Figure 8 As shown, in one embodiment, the sealing membrane 120 is further provided with a third vent 125, which communicates with the mixing chamber 115. When mixing the sample and diluent, the gas in the mixing chamber 115 can be discharged through the third vent 125.

[0143] In one implementation, the microfluidic chip 100 is used for the detection of blood samples, meaning the sample added to the injection chamber 111 is a blood sample. Of course, in specific applications, the microfluidic chip 100 can also be used for the detection of other samples, such as urine samples.

[0144] As one implementation method, the microfluidic chip 100 is used for the detection of whole blood samples, plasma samples, or serum samples. That is, the microfluidic chip 100 can simultaneously meet the detection requirements of whole blood samples, plasma samples, and serum samples, and has a wide range of applications.

[0145] Reference Figure 8 , Figure 10 and Figure 11 As shown, this embodiment also provides a microfluidic analysis system, which includes a turntable 200, an optical detection component 300, a rotary drive mechanism 400, and the aforementioned microfluidic chip 100. The turntable 200 is used to load at least one microfluidic chip 100, the rotary drive mechanism 400 is used to drive the turntable 200 to rotate the microfluidic chip 100, and the optical detection component 300 is used to perform optical detection on the sample in the reaction detection chamber 117. Because the microfluidic analysis system uses the aforementioned microfluidic chip 100, the number of microfluidic chips 100 loaded can be customized according to detection requirements, thereby avoiding waste of microfluidic chips 100, samples, reagents, and diluents. Furthermore, during the detection process, only the operator needs to add the sample and diluent, making the operation simple and convenient.

[0146] In one implementation, the turntable 200 has multiple accommodating positions arranged sequentially along the circumference. Each accommodating position is used to accommodate a microfluidic chip 100 or a counterweight component with the same outer contour and weight as the microfluidic chip 100. The counterweight component is mainly used to ensure the balance of the microfluidic chip 100 after it is mounted on the turntable 200. For example, if only one microfluidic chip 100 is needed to meet the detection requirements, a counterweight component can be configured and arranged symmetrically with the microfluidic chip 100 on the turntable 200.

[0147] Reference Figure 9 , Figure 10 and Figure 11As shown, in one embodiment, the optical detection assembly 300 includes a light emitter 310 and a light receiver 320. The light emitter 310 is disposed above the turntable 200 to irradiate light toward the sample in the reaction detection chamber 117. The light receiver 320 is disposed below the turntable 200 and directly below the light emitter 310 to receive light irradiated by the light emitter 310 through the microfluidic chip 100. The rotation drive mechanism 400 is used to drive the turntable 200 to rotate the microfluidic chip 100 to respectively realize: quantitative analysis of the sample and diluent, mixing of the sample and diluent, distribution of the mixture, and rotating the reaction detection chamber 117 to directly below the light receiver 320. Specifically, the rotary drive mechanism 400 first drives the turntable 200 to perform a first centrifugal rotation of the microfluidic chip 100 to achieve quantitative analysis of the sample and diluent; then, it drives the turntable 200 to perform a second centrifugal rotation of the microfluidic chip 100 to achieve mixing of the sample and diluent; then, it drives the turntable 200 to perform a third centrifugal rotation of the microfluidic chip 100 to achieve distribution of the mixture; finally, it drives the turntable 200 to rotate the microfluidic chip 100 so that each reaction detection chamber 117 rotates sequentially to be directly below the photoreceiver 320 for detection.

[0148] In one implementation, the turntable 200 has six accommodating positions arranged sequentially along the circumference. Each microfluidic chip 100 has a central angle of 60°, and the counterweight also has a central angle of 60°. The turntable 200 can carry six microfluidic chips 100 for testing at a time; alternatively, it can carry five microfluidic chips 100 and one counterweight at a time; alternatively, it can carry four symmetrically arranged microfluidic chips 100 for testing at a time; alternatively, it can carry three microfluidic chips 100 and one counterweight at a time; alternatively, it can carry two symmetrically arranged microfluidic chips 100 for testing at a time; alternatively, it can carry one microfluidic chip 100 and one counterweight at a time.

[0149] In one implementation, when the turntable 200 carries two or more microfluidic chips 100 for testing at one time, the samples in the two microfluidic chips 100 can be samples from the same patient or samples from different patients.

[0150] In one implementation, the microfluidic analysis system also includes a controller 500, which is electrically connected to the optical detection component 300 and the rotary drive mechanism 400. The controller 500 is used to control the rotary drive mechanism 400 to drive the turntable 200 to rotate and stop, and to analyze the detection data based on the feedback information from the optical detection component 300.

[0151] In one implementation, the time for the rotary drive mechanism 400 to drive the turntable 200 to rotate during the sample and diluent quantification stage is greater than the time for the rotary drive mechanism 400 to drive the turntable 200 to rotate during the sample and diluent mixing stage, and also greater than the time for the rotary drive mechanism 400 to drive the turntable 200 to rotate during the mixture dispensing stage.

[0152] As one implementation method, the microfluidic chip 100 and microfluidic analysis system provided in this embodiment can be applied to the detection of animal samples as well as human samples.

[0153] As one implementation method, the workflow for sample detection using the microfluidic analysis system provided in this embodiment is as follows:

[0154] (1) First step; refer to Figure 2 As shown, a certain amount (e.g., between 20 μL and 50 μL) of sample (whole blood sample, plasma sample, or serum sample) is added into the sample injection chamber 111 through the sample injection port 121, and a certain amount (e.g., between 50 μL and 100 μL) of diluent is added into the diluent injection chamber 113 through the diluent injection port 122.

[0155] (2) Second step: Refer to Figure 2 , Figure 3 and Figure 9 As shown, the drive turntable 200 rotates at a first preset speed (e.g., between 3000 rpm and 6000 rpm) for a first preset time (e.g., 3 min to 5 min), so that the sample in the sample inlet chamber 111 flows into the first sample quantification chamber 112 and the second sample quantification chamber 105, and the excess sample flows into the second overflow chamber 119. Simultaneously, the gas in the first sample quantification chamber 112, the second sample quantification chamber 105, and the second overflow chamber 119 is discharged from the second vent 124. Furthermore, the presence of liquid in the sample determination chamber 101 can be determined by the optical detection component 300. If liquid is present, the sample volume is considered sufficient; otherwise, the sample volume is considered insufficient. If the sample is a whole blood sample, during the later stages of centrifugation, the plasma will concentrate in the first sample quantification chamber 112, and the red blood cells will concentrate in the second sample quantification chamber 105.

[0156] While the sample is being quantified, the diluent in the diluent chamber 113 flows into the diluent quantification chamber 114, and excess diluent flows into the diluent judgment chamber 102 and the first overflow chamber 118. Simultaneously, the gas originally in the diluent quantification chamber 114, the diluent judgment chamber 102, and the first overflow chamber 118 is discharged through the first vent 123. The presence of liquid in the diluent judgment chamber 102 can be determined by the optical detection component 300. If liquid is present, it is determined that the diluent is sufficient; otherwise, it is determined that the diluent is insufficient.

[0157] During the later stages of the second step of the centrifugation process, plasma enters the sample drainage capillary 107. Due to the outward centrifugal force, the sample can only remain at position a1. Similarly, diluent enters the diluent drainage capillary 108, and the diluent can only remain at position b1.

[0158] (3) Third step: Refer to Figure 3 , Figure 4 and Figure 9 As shown, when turntable 200 stops rotating and centrifugal force disappears, the plasma in sample drainage capillary 107 fills the sample drainage capillary 107 under capillary force, and the plasma moves to position a2 of sample drainage capillary 107. Similarly, diluent fills diluent drainage capillary 108, and the diluent moves to position b2 of diluent drainage capillary 108.

[0159] (4) Fourth step, refer to Figure 4 , Figure 5 and Figure 9 As shown, the turntable 200 rotates at a second preset speed (e.g., between 3000 rpm and 5000 rpm, the second preset speed can be less than, equal to, or greater than the first preset speed) for a second preset time (e.g., 10 sec to 60 sec, the second preset time is preferably less than the first preset time). Plasma in the first sample quantification chamber 112 flows into the mixing chamber 115 through the sample drainage capillary 107 under siphon action. Similarly, diluent in the diluent quantification chamber 114 flows into the mixing chamber 115 through the diluent drainage capillary 108, and gas in the mixing chamber 115 is discharged from the third vent 125. The turntable 200 mixes the diluent and plasma under rapid acceleration and slow deceleration. Due to the outward centrifugal force, the mixture can only remain at position c1 of the mixing drainage capillary 109.

[0160] (5) Fifth step: Refer to Figure 5 , Figure 6 and Figure 9 As shown, the turntable 200 stops rotating, the centrifugal force disappears, the mixture in the mixed liquid drainage capillary 109 fills the mixed liquid drainage capillary 109, and the mixed liquid moves to position c2 of the mixed liquid drainage capillary 109.

[0161] (6) Step Six: Refer to Figure 6 , Figure 7 and Figure 9As shown, the turntable 200 rotates at a third preset speed (e.g., 3000 rpm to 5000 rpm, the third preset speed can be less than, equal to, or greater than the first preset speed) for a third preset time (e.g., 10 sec to 60 sec, the third preset time is preferably less than the first preset time). The mixture in the mixing chamber 115 flows into the distribution chamber 116 through the mixture drainage capillary 109. The initial liquid entering the distribution chamber 116 from the mixture drainage capillary 109 preferentially enters the initial liquid chamber 1001, which is mainly used to load the unmixed liquid in the mixture drainage capillary 109. The mixture then flows into the reaction detection chamber 117, and the excess mixture in the distribution chamber 116 flows into the second overflow chamber 119. At the same time, the gas originally in the distribution chamber 116 and the reaction detection chamber 117 is discharged from the second vent 124 through the dilution overflow channel 103. The lyophilized reagent bulbs contained in the reaction detection chamber 117 can react with the mixed solution, and different items can be detected by acquiring optical signals.

[0162] The microfluidic chip 100 provided in this embodiment eliminates the need for quantitative addition of diluent and sample, and can simultaneously detect different types of samples such as whole blood, plasma, and serum. In operation, the operator only needs to add a certain range of sample and a certain range of diluent to the microfluidic chip 100 to complete the operation. The operation is very simple and convenient, requiring only a small sample volume and providing accurate quantification. Each microfluidic chip 100 constitutes an independently partitioned and independently detectable sub-disc. Multiple microfluidic chips 100 can be freely combined for detection, and the operator can customize the selection of microfluidic chips 100 for retesting, reducing retesting costs and unnecessary waste of consumables.

[0163] Example 2:

[0164] Reference Figure 1 , Figure 8 and Figure 12 As shown, the microfluidic chip 100 and microfluidic analysis system provided in this embodiment differ from those in Embodiment 1 mainly in the shape of the microfluidic chip 100. Specifically, the microfluidic chip 100 in Embodiment 1 is fan-shaped, while the microfluidic chip 100 in this embodiment is trapezoidal.

[0165] In one implementation, the first edge 1005 and the second edge 1006 are two parallel straight edges, and the third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a gradually decreasing spacing. In this implementation, the microfluidic chip 100 has a trapezoidal structure.

[0166] Of course, in specific applications, the shape of the microfluidic chip 100 is not limited to a fan shape or a trapezoid. For example, as an alternative implementation, the microfluidic chip 100 can also be a rectangular structure. In this alternative, the first edge 1005 and the second edge 1006 are also two parallel straight edges, and the third edge 1007 and the fourth edge 1008 extend from the second edge 1006 to the first edge 1005 with a constant spacing.

[0167] Apart from the differences mentioned above, the microfluidic chip 100 and other parts of the microfluidic analysis system provided in this embodiment can be referred to in Embodiment 1, and will not be described in detail here.

[0168] Example 3:

[0169] Reference Figure 1 , Figure 8 and Figure 13 As shown, the microfluidic chip 100 and microfluidic analysis system provided in this embodiment differ from those in Embodiment 1 mainly in the way the overflow chamber is set. Specifically, in Embodiment 1, the overflow chamber for diluent and the overflow chamber for mixed solution are shared, while the overflow chamber for sample is set independently; whereas in this embodiment, the overflow chamber for sample and the overflow chamber for mixed solution are shared, while the overflow chamber for diluent is set independently.

[0170] In one embodiment, the non-circular chip body 110 comprises a sample inlet chamber 111, a first sample quantification chamber 112, a diluent inlet chamber 113, a diluent quantification chamber 114, a mixing chamber 115, a dispensing chamber 116, a reaction detection chamber 117, a third overflow chamber 1011, and a fourth overflow chamber 1012. The third overflow chamber 1011 is connected to the diluent quantification chamber 114 to collect the diluent overflowing from the diluent quantification chamber 114. The fourth overflow chamber 1012 is connected to both the first sample quantification chamber 112 and the dispensing chamber 116 to collect the sample overflowing from the first sample quantification chamber 112 and the mixed solution overflowing from the dispensing chamber 116. In this embodiment, the arrangement and principle of the sample inlet chamber 111, the first sample quantification chamber 112, the diluent inlet chamber 113, the diluent quantification chamber 114, the mixing chamber 115, the dispensing chamber 116, and the reaction detection chamber 117 are the same as in Embodiment 1, and will not be described in detail here. In this embodiment, since the fourth overflow chamber 1012 is used to collect both the sample overflowing from the first sample quantification chamber 112 and the mixture overflowing from the dispensing chamber 116, it is equivalent to combining the overflow chamber for sample quantification and the overflow chamber for mixture into one. This can also help reduce the number of overflow chambers, thereby simplifying the structure of the microfluidic chip 100 and improving the structural compactness of the microfluidic chip 100, and ultimately facilitating the miniaturization design of the microfluidic chip 100.

[0171] Apart from the differences mentioned above, the microfluidic chip 100 and other parts of the microfluidic analysis system provided in this embodiment can be referred to in Embodiment 1 or Embodiment 2, and will not be described in detail here.

[0172] The above description is merely a preferred embodiment of this application and does not limit the patent scope of this application. Any equivalent structural transformations made based on the inventive concept of this application and the contents of the specification and drawings of this application, or direct / indirect applications in other related technical fields, are included within the patent protection scope of this application.

Claims

1. A microfluidic chip, characterized in that: Includes a non-circular chip body, which is formed with a sample injection chamber, a first sample quantification chamber, a diluent injection chamber, a diluent quantification chamber, a mixing chamber, a dispensing chamber, a reaction detection chamber, a first overflow chamber, and a second overflow chamber; The sample inlet chamber is used to store the sample entering the microfluidic chip; The first sample quantification chamber is connected to the sample injection chamber for quantifying the sample from the sample injection chamber when the microfluidic chip is centrifuged and rotated. The diluent chamber is used to store the diluent that enters the microfluidic chip; The diluent metering chamber is connected to the diluent inlet chamber to meter the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged and rotated. The mixing chamber is connected to the first sample metering chamber and the diluent metering chamber, respectively, to receive and mix the sample entering from the first sample metering chamber and the diluent entering from the diluent metering chamber when the microfluidic chip is centrifuged and rotated. The dispensing chamber is connected to the mixing chamber and the reaction detection chamber respectively, so as to receive the mixture formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged and rotated, and dispense the mixture into the reaction detection chamber; The reaction detection chamber is used for the reaction of reagents with the mixture to form a sample; The first overflow chamber is connected to the diluent metering chamber and the dispensing chamber, respectively, for collecting the diluent overflowing from the diluent metering chamber and the mixture overflowing from the dispensing chamber; The second overflow chamber is connected to the first sample quantification chamber for collecting the sample overflowing from the first sample quantification chamber.

2. The microfluidic chip as described in claim 1, characterized in that: The non-circular chip body also forms a sample judgment cavity, which is connected to the second overflow cavity for the optical detection component to detect whether there is a sample overflowing from the first sample quantification cavity; The distance from the sample judgment cavity to the rotation center axis of the microfluidic chip is the same as the distance from the reaction detection cavity to the rotation center axis of the microfluidic chip.

3. The microfluidic chip as described in claim 2, characterized in that: The non-circular chip body also forms a diluent detection cavity, which is connected to the first overflow cavity, so as to allow the optical detection component to detect whether there is diluent overflowing from the diluent metering cavity; The distance from the dilution determination chamber to the rotation center axis of the microfluidic chip is the same as the distance from the reaction detection chamber to the rotation center axis of the microfluidic chip.

4. The microfluidic chip as described in claim 3, characterized in that: The distance from the dilution chamber to the rotation center axis of the microfluidic chip is greater than the distance from the first overflow chamber to the rotation center axis of the microfluidic chip.

5. The microfluidic chip as described in claim 3 or 4, characterized in that: The sample determination chamber and the diluent determination chamber are located on opposite sides of the reaction detection chamber pair, respectively, along the direction of centrifugal rotation of the microfluidic chip.

6. The microfluidic chip according to any one of claims 1 to 4, characterized in that: The non-circular chip body also forms a first channel and a second channel. The two ends of the first channel are respectively connected to the distribution cavity and the first overflow cavity, and the two ends of the second channel are respectively connected to the distribution cavity and the reaction detection cavity. The width of the first channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

7. The microfluidic chip according to any one of claims 1 to 4, characterized in that: The non-circular chip body has a first plate surface and a second plate surface arranged opposite to each other. The sample injection chamber, the first sample quantification chamber, the diluent injection chamber, the diluent quantification chamber, the mixing chamber, the dispensing chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber are all recessed from the first plate surface toward the second plate surface, and all have a distance between them and the second plate surface. The microfluidic chip further includes a sealing membrane, which is attached to the first plate surface to at least cover the sample injection chamber, the first sample quantification chamber, the diluent injection chamber, the diluent quantification chamber, the mixing chamber, the dispensing chamber, the reaction detection chamber, the first overflow chamber, and the second overflow chamber. The sealing membrane has a sample injection hole that extends through the sample inlet chamber at a location corresponding to the sample inlet chamber. The sample injection hole is connected to the sample inlet chamber for injecting the sample into the sample inlet chamber.

8. The microfluidic chip as described in claim 7, characterized in that: The sealing membrane has a diluent injection hole penetrating through it at a position corresponding to the diluent inlet chamber. This diluent injection hole communicates with the diluent inlet chamber for injecting diluent into it; or... A diluent packet is placed inside the diluent inlet chamber.

9. The microfluidic chip as described in claim 7, characterized in that: The non-circular chip body also forms a diluent overflow channel and a sample overflow channel. The diluent overflow channel is connected between the diluent metering chamber and the first overflow chamber, and the sample overflow channel is connected between the first sample metering chamber and the second overflow chamber. The sealing membrane is also provided with a first vent, a second vent, and a third vent. The first vent is connected to the diluent overflow channel, the second vent is connected to the sample overflow channel, and the third vent is connected to the mixing chamber.

10. The microfluidic chip according to any one of claims 1 to 4, characterized in that: The non-circular chip body also forms a second sample quantification cavity, a sample quantification channel, and a sample drainage capillary, with the two ends of the sample quantification channel respectively connected to the first sample quantification cavity and the second sample quantification cavity; The distance from the sample quantification channel to the rotation center axis of the microfluidic chip is greater than the distance from the first sample quantification cavity to the rotation center axis of the microfluidic chip, and less than the distance from the second sample quantification cavity to the rotation center axis of the microfluidic chip. The two ends of the sample drainage capillary are respectively connected to the sample quantification channel and the mixing chamber, and the sample drainage capillary has a first bend. The distance from the first bend to the rotation center axis of the microfluidic chip is less than the distance from the first sample quantification chamber to the rotation center axis of the microfluidic chip.

11. The microfluidic chip according to any one of claims 1 to 4, characterized in that: The non-circular chip body also forms a mixed liquid drainage capillary and an initial liquid cavity; The two ends of the mixture drainage capillary are respectively connected to the mixing chamber and the dispensing chamber, and the mixture drainage capillary has a second bend. The distance from the second bend to the rotation center axis of the microfluidic chip is less than the distance from the mixing chamber to the rotation center axis of the microfluidic chip. The initial liquid chamber is connected to the end of the distribution chamber near the end of the mixed liquid drainage capillary, so as to at least collect the initial liquid entering the distribution chamber from the mixed liquid drainage capillary. The distance from the initial liquid chamber to the rotation center axis of the microfluidic chip is less than the distance from the reaction detection chamber to the rotation center axis of the microfluidic chip.

12. The microfluidic chip as described in claim 11, characterized in that: The volume of the initial liquid chamber is smaller than the volume of the reaction detection chamber; and / or, The non-circular chip body also forms a second channel and a third channel. The two ends of the second channel are respectively connected to the dispensing cavity and the reaction detection cavity. The two ends of the third channel are respectively connected to the dispensing cavity and the initial liquid cavity. The width of the third channel in the centrifugal rotation direction of the microfluidic chip is equal to the width of the second channel in the centrifugal rotation direction of the microfluidic chip.

13. The microfluidic chip as described in claim 1, characterized in that: The non-circular chip body includes a first edge and a second edge, the first edge and the second edge are arranged opposite to each other at a distance, the first edge is located at one end of the microfluidic chip close to the rotation center axis of the microfluidic chip's centrifugal rotation, and the second edge is located at one end of the microfluidic chip away from the rotation center axis of the microfluidic chip's centrifugal rotation; The diluent inlet chamber, the diluent metering chamber, the mixing chamber, the dispensing chamber, and the first overflow chamber are arranged sequentially between the first edge and the second edge; The injection chamber, the first sample quantification chamber, the mixing chamber, the dispensing chamber, and the second overflow chamber are arranged sequentially between the first edge and the second edge.

14. The microfluidic chip as described in claim 13, characterized in that: The length of the second edge is greater than the length of the first edge.

15. The microfluidic chip as described in claim 13, characterized in that: The first edge and the second edge are two concentric arc-shaped edges; or, The first edge and the second edge are two parallel straight lines.

16. The microfluidic chip according to any one of claims 13 to 15, characterized in that: The non-circular chip body also includes a third edge and a fourth edge; The third edge and the fourth edge are spaced apart and opposite to each other. The third edge extends from one end of the first edge to one end of the second edge, and the fourth edge extends from the other end of the first edge to the other end of the second edge. The diluent inlet chamber, the diluent metering chamber, one end of the mixing chamber, one end of the dispensing chamber, and the first overflow chamber are arranged sequentially along the third edge; The injection chamber, the first sample quantification chamber, and the second overflow chamber are arranged sequentially along the fourth edge.

17. The microfluidic chip as described in claim 16, characterized in that: The angle formed by the third edge and the fourth edge is greater than 0° and less than or equal to 90°.

18. The microfluidic chip as described in claim 17, characterized in that: The angle formed by the third edge and the fourth edge is 60°±15°.

19. The microfluidic chip according to any one of claims 1 to 4 or any one of claims 13 to 15, characterized in that: The number of reaction detection cavities formed by the non-circular chip body is less than or equal to six.

20. The microfluidic chip as described in claim 19, characterized in that: The number of reaction detection cavities formed by the non-circular chip body is two, three, four, or five.

21. A microfluidic chip, characterized in that: It includes a non-circular chip body, which is formed with a sample injection chamber, a first sample quantification chamber, a diluent injection chamber, a diluent quantification chamber, a mixing chamber, a dispensing chamber, a reaction detection chamber, a third overflow chamber, and a fourth overflow chamber; The first sample quantification chamber is connected to the sample injection chamber for quantifying the sample from the sample injection chamber when the microfluidic chip is centrifuged and rotated. The diluent metering chamber is connected to the diluent inlet chamber to meter the diluent from the diluent inlet chamber when the microfluidic chip is centrifuged and rotated. The mixing chamber is connected to the first sample metering chamber and the diluent metering chamber, respectively, to receive and mix the sample entering from the first sample metering chamber and the diluent entering from the diluent metering chamber when the microfluidic chip is centrifuged and rotated. The dispensing chamber is connected to the mixing chamber and the reaction detection chamber respectively, so as to receive the mixture formed by mixing the sample and the diluent from the mixing chamber when the microfluidic chip is centrifuged and rotated, and dispense the mixture into the reaction detection chamber; The reaction detection chamber is used for the reaction of reagents with the mixture to form a sample; The third overflow chamber is connected to the diluent metering chamber for collecting the diluent overflowing from the diluent metering chamber; The fourth overflow chamber is connected to the first sample quantification chamber and the distribution chamber, respectively, for collecting the sample overflowing from the first sample quantification chamber and collecting the mixture overflowing from the distribution chamber.

22. A microfluidic analysis system, characterized in that: Includes a turntable, an optical detection assembly, a rotary drive mechanism, and a microfluidic chip as described in any one of claims 1 to 21; The turntable has multiple accommodating positions arranged sequentially along the circumference, each of which is used to accommodate one of the microfluidic chips or a counterweight component with the same outer contour and weight as the microfluidic chip. The optical detection assembly includes a light emitter and a light receiver. The light emitter is positioned above the turntable to irradiate light toward the sample in the reaction detection chamber. The light receiver is located below the turntable and directly below the light emitter, for receiving light emitted by the light emitter that passes through the microfluidic chip; The rotary drive mechanism is used to drive the turntable to rotate the microfluidic chip, so as to respectively realize: quantitative analysis of sample and diluent, mixing of sample and diluent, distribution of mixture, and rotating the reaction detection cavity to directly below the photodetector.

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