Immunohistochemical multi-staining section as well as preparation method and application thereof
Through immunohistochemistry superposition staining technology, multiple antibodies are labeled on the same section at the same time, solving the problem that the relationship between tumor tissue and nerve and vascular invasion is difficult to intuitively interpret, achieving the accurate diagnosis of epithelial-derived malignant tumors, and meeting the needs of pathologists.
Patent Information
- Application Number
- CN202510510627.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-23
- Publication Date
- 2025-05-27
AI Technical Summary
In the prior art, the invasion relationship between tumor tissue and nerves and blood vessels is difficult to intuitively interpret on a single section, and a single staining is limited by the size of the lesion area, making it difficult to meet the diagnostic needs.
Immunohistochemical superposition staining (sporn staining) technology is used to label multiple antibodies on the same tissue section at the same time, and different colors are used to accurately evaluate the relationship between tumor cells and nerves and blood vessels. Specifically, AE1/AE3 antibodies are used to label epithelial-derived tumors, S-100 or NSE antibodies are used to label neural tissue, and CD31 or CD34 antibodies are used to label vascular structures.
The invasion relationship between epithelial-derived malignant tumor components and nerves and blood vessels is achieved on a section, which meets the precise diagnosis needs of pathologists, and reduces the sample size needs and the workload of pathologists.