Pet wound repairing composition and preparation method thereof

Abstract

The invention relates to the technical field of pet wound repair, in particular to a pet wound repair composition and a preparation method thereof. The pet wound repair composition is prepared from hydrolyzed II-type collagen, nano-calcium-based montmorillonite, a curcumin-phospholipid compound and euphausia superba oil microcapsule powder; according to the present invention, the euphausia superba oil microcapsule powder is added to the wound repairing agent, such that the nutritional components required by the wound repairing are tightly adsorbed around the wound so as to improve the local concentration of the nutritional components, the euphausia superba oil microcapsule powder is rich in Omega-3 fatty acid and astaxanthin so as to regulate the inflammatory response, repair the damaged skin barrier and enhance the immunity, and the wound healing can be promoted through the curcumin-phospholipid complex so as to achieve the wound repairing effect; the composition has the advantages that the composition is simple in preparation process and low in cost, infection risk is reduced, hydrolyzed II-type collagen is used as a main component for cartilage repair, cell proliferation and differentiation can be quickly stimulated, wound healing is accelerated, and the composition is beneficial to quicker health recovery of pets after operation and reduction of complications.

Description

Technical Field

[0001] The present invention relates to the technical field of pet trauma repair, and particularly relates to a pet trauma repair composition and a preparation method thereof. Background Art

[0002] During the process of pet raising, many pets will suffer from traumas due to various accidents, such as bites, scratches, cuts, surgical traumas, etc. After a pet suffers a trauma, the usual treatment measure is to use antibiotics in large doses for a long time until the wound heals; at the same time, a certain diet management is used in combination to improve the nutritional level of the pet, so as to promote the healing of the pet's wound and the recovery of the body. In the prior art, there are great differences between the daily nutrition of pets and the nutrition during the trauma recovery period. For nutritional supplements that balance the daily nutrition of pets, they usually focus on promoting the appetite of pets, conditioning the pet's stomach, brightening the pet's hair color, etc., and they are completely inapplicable to the nutritional needs of pets during the trauma recovery period.

[0003] However, traditional postoperative nutritional agents for pets mostly adopt single components (such as glucosamine or Omega-3), and there are problems such as low absorption rate (<15%) and slow onset of action (>14 days). Summary of the Invention

[0004] The purpose of the present invention is to provide a pet trauma repair composition and a preparation method thereof, so as to solve the problems that traditional postoperative nutritional agents for pets mostly adopt single components and have low absorption rate and slow onset of action.

[0005] To achieve the above purpose, the present invention provides a pet trauma repair composition, which includes hydrolyzed type II collagen, nano calcium-based montmorillonite, curcumin-phospholipid complex, and Antarctic krill oil microcapsule powder. The mass ratio of the hydrolyzed type II collagen to the nano calcium-based montmorillonite is 5:1 to 1:1. The curcumin-phospholipid complex accounts for 0.05-0.1% of the total amount of the pet trauma repair composition, and the Antarctic krill oil microcapsule powder accounts for 5-15% of the total amount of the pet trauma repair composition.

[0006] Among them, the molecular weight of the hydrolyzed type II collagen is 2000-3000 Da.

[0007] Among them, the particle size of the nano calcium-based montmorillonite is ≤100 nm, and the specific surface area is ≥200 m 2 / g.

[0008] The present invention also provides a preparation method of a pet trauma repair composition for preparing the pet trauma repair composition as described above, which is characterized by including the following steps:

[0009] Prepare type II collagen by using a bovine tendon enzymatic hydrolysis process;

[0010] Hydrolyze type II collagen using a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da;

[0011] Convert nano sodium montmorillonite into the nano calcium montmorillonite using an ion exchange method;

[0012] Weigh a quantitative amount of the curcumin - phospholipid complex and the Antarctic krill oil microcapsule powder, and mix them with the prepared hydrolyzed type II collagen and the nano calcium montmorillonite to obtain a mixture;

[0013] Using a pH - responsive microcapsule technology, mix the mixture with a dispersion liquid, and after coating and curing treatments, prepare the pet wound repair composition.

[0014] Among them, the specific steps of the step "Prepare type II collagen using a bovine tendon enzymatic hydrolysis process" are:

[0015] Select fresh or frozen - stored bovine tendons as raw materials, and perform pretreatment on the raw materials;

[0016] After cleaning the pretreated bovine tendons, put them into a container containing ethanol for soaking treatment;

[0017] Put the pretreated bovine tendons into an enzymatic hydrolysis tank, add an enzymatic hydrolysis solution, ensure that the bovine tendons are completely immersed in the enzymatic hydrolysis solution, and perform an enzymatic hydrolysis reaction to release collagen;

[0018] After the enzymatic hydrolysis ends, extract and purify the collagen in the enzymatic hydrolysis solution to obtain type II collagen.

[0019] Among them, the specific steps of the step "Hydrolyze type II collagen using a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da" are:

[0020] Perform pretreatment on type II collagen, and add the pretreated type II collagen to an enzymatic hydrolysis container;

[0021] Add trypsin to the enzymatic hydrolysis container, and after its reaction is completed, add bromelain to achieve step - by - step enzymatic hydrolysis;

[0022] After the enzymatic hydrolysis is completed, use separation and purification techniques to separate the active peptide segments with a molecular weight of 2000 - 2800 Da from the reaction system to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da.

[0023] Among them, in the step of "adding trypsin to the enzymatic hydrolysis container, and after the reaction is completed, adding bromelain to achieve stepwise enzymatic hydrolysis", the degree of hydrolysis DH needs to be measured by monitoring the pH value change and nitrogen release amount of the reaction system to ensure that it is within the range of 18-22%.

[0024] Among them, the specific steps of the step of "converting nano sodium-based montmorillonite into the nano calcium-based montmorillonite by the ion exchange method" are as follows:.

[0025] Disperse the nano sodium-based montmorillonite in an appropriate amount of deionized water to form a uniform suspension;

[0026] Slowly add the pretreated montmorillonite suspension to the calcium salt solution for ion exchange reaction;

[0027] After the reaction is completed, wash the montmorillonite suspension with deionized water to remove unreacted calcium salt and other impurities, and dry the washed montmorillonite to obtain the nano calcium-based montmorillonite.

[0028] Among them, the specific steps of the step of "using the pH-responsive microcapsule technology to mix the mixture with the dispersion liquid, and after coating and curing treatment, preparing the pet wound repair composition stone" are as follows:

[0029] Dissolve sodium polystyrene sulfonate and poly (lactic-co-glycolic acid) copolymer in an organic solvent to obtain an organic phase, drop the organic phase into deionized water, and stir and mix evenly. Remove the organic solvent in the system by evaporation to obtain a PLGA / PSS nanoparticle dispersion;

[0030] Add the mixture to the PLGA / PSS nanoparticle dispersion, mix and incubate for a period of time to load the drug onto the nanoparticles to obtain drug-loaded nanoparticles;

[0031] Drop the drug-loaded nanoparticles into the pectin solution, stir and mix, remove the supernatant by centrifugation, and then add deionized water to redisperse to obtain a pectin-coated drug-loaded nanoparticle dispersion;

[0032] Add the pectin-coated drug-loaded nanoparticle dispersion to the polyacrylic resin solution, stir and mix, add the mixed solution to the HCl solution, stir and mix to solidify the microcapsules to obtain a pH-responsive microcapsule containing drug powder;

[0033] After drying the pH-responsive microcapsule containing drug powder, prepare the pet wound repair composition stone.

[0034] A pet wound repair composition and its preparation method of the present invention include hydrolyzed type II collagen, nano calcium-based montmorillonite, curcumin-phospholipid complex, and Antarctic krill oil microcapsule powder. Through the adsorption of the nano calcium-based montmorillonite, the nutritional components required for wound repair are tightly adsorbed around the wound, increasing the local concentration of the nutritional components. Since the Antarctic krill oil microcapsule powder is rich in Omega-3 fatty acids and astaxanthin, it can regulate the inflammatory response, repair the damaged skin barrier, enhance immunity, and the curcumin-phospholipid complex can promote wound healing and reduce the risk of infection. The hydrolyzed type II collagen, as the main component for cartilage repair, can quickly stimulate cell proliferation and differentiation, accelerate wound healing. Using this composition helps pets recover faster after surgery and reduces the occurrence of complications. Brief Description of the Drawings

[0035] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0036] Figure 1 It is a schematic structural diagram of the pet wound repair composition and its preparation method provided by the present invention. Detailed Embodiments

[0037] The following will describe in detail the embodiments of the present invention. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present invention and should not be construed as a limitation of the present invention.

[0038] The present invention provides a pet wound repair composition. The pet wound repair composition includes hydrolyzed type II collagen, nano calcium-based montmorillonite, curcumin-phospholipid complex, and Antarctic krill oil microcapsule powder. The mass ratio of the hydrolyzed type II collagen to the nano calcium-based montmorillonite is 5:1 to 1:1. The curcumin-phospholipid complex accounts for 0.05 - 0.1% of the total amount of the pet wound repair composition, and the Antarctic krill oil microcapsule powder accounts for 5 - 15% of the total amount of the pet wound repair composition.

[0039] In this embodiment, through the adsorption of the nano calcium-based montmorillonite, the nutritional components required for wound repair are tightly adsorbed around the wound, increasing the local concentration of the nutritional components. Since the Antarctic krill oil microcapsule powder is rich in Omega-3 fatty acids and astaxanthin, it can regulate the inflammatory response, repair the damaged skin barrier, enhance immunity, and the curcumin-phospholipid complex can promote wound healing and reduce the risk of infection. As the main component for cartilage repair, the hydrolyzed type II collagen can rapidly stimulate cell proliferation and differentiation, accelerating wound healing. Using this composition helps pets recover health faster after surgery and reduces the occurrence of complications.

[0040] Further, the molecular weight of the hydrolyzed type II collagen is 2000 - 3000 Da.

[0041] Further, the particle size of the nano calcium-based montmorillonite is ≤100 nm, and the specific surface area is ≥200 m 2 / g.

[0042] Please refer to Figure 1 , the present invention also includes a preparation method of a pet wound repair composition for preparing the pet wound repair composition as described above, which is characterized by including the following steps:

[0043] S1: Prepare type II collagen by using a bovine tendon enzymatic hydrolysis process;

[0044] S2: Hydrolyze type II collagen by using a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da;

[0045] S3: Convert nano sodium-based montmorillonite into the nano calcium-based montmorillonite by using an ion exchange method;

[0046] S4: Weigh a quantitative amount of the curcumin-phospholipid complex and the Antarctic krill oil microcapsule powder, and mix them with the prepared hydrolyzed type II collagen and the nano calcium-based montmorillonite to obtain a mixture;

[0047] S5: Use a pH-responsive microencapsulation technology to mix the mixture with a dispersion liquid, and after coating and curing treatments, prepare the pet wound repair composition.

[0048] In this embodiment, first, type II collagen is prepared by the enzymatic hydrolysis process of bovine Achilles tendon, and the type II collagen is hydrolyzed by the composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da. At the same time, nano-sodium montmorillonite is converted into the nano-calcium montmorillonite by the ion exchange method. Then, a quantitative amount of the curcumin-phospholipid complex and the Antarctic krill oil microcapsule powder are weighed and mixed with the prepared hydrolyzed type II collagen and the nano-calcium montmorillonite to obtain a mixture. Finally, by using the pH-responsive microcapsule technology, the mixture is mixed with a dispersion liquid, and after coating and curing treatments, the pet wound repair composition is prepared.

[0049] Further, the specific steps of the step "preparing type II collagen by the enzymatic hydrolysis process of bovine Achilles tendon" are as follows:

[0050] Select fresh or frozen-preserved bovine Achilles tendon as the raw material, and perform pretreatment on the raw material;

[0051] After washing the pretreated bovine Achilles tendon, put it into a container containing ethanol and perform an immersion treatment;

[0052] Put the pretreated bovine Achilles tendon into an enzymatic hydrolysis tank, add an enzymatic hydrolysis solution, ensure that the bovine Achilles tendon is completely immersed in the enzymatic hydrolysis solution, and perform an enzymatic hydrolysis reaction to release collagen;

[0053] After the enzymatic hydrolysis is completed, extract and purify the collagen in the enzymatic hydrolysis solution to obtain type II collagen.

[0054] Further, the specific steps of the step "hydrolyzing type II collagen by the composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da" are as follows:

[0055] Perform pretreatment on type II collagen, and add the pretreated type II collagen into an enzymatic hydrolysis container;

[0056] Add trypsin into the enzymatic hydrolysis container, and after its reaction is completed, add bromelain to achieve stepwise enzymatic hydrolysis;

[0057] After the enzymatic hydrolysis is completed, use separation and purification techniques to separate the active peptide segments with a molecular weight of 2000 - 2800 Da from the reaction system to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 - 2800 Da.

[0058] Further, in the step "add trypsin into the enzymatic hydrolysis container, and after its reaction is completed, add bromelain to achieve stepwise enzymatic hydrolysis", it is necessary to measure the degree of hydrolysis DH by monitoring the pH value change and nitrogen release amount of the reaction system to ensure that it is within the range of 18 - 22%.

[0059] Further, the specific steps of the step "converting nano sodium-based montmorillonite into the nano calcium-based montmorillonite by an ion exchange method" are as follows:

[0060] Disperse the nano sodium-based montmorillonite in an appropriate amount of deionized water to form a uniform suspension;

[0061] Slowly add the pretreated montmorillonite suspension to the calcium salt solution for an ion exchange reaction;

[0062] After the reaction ends, wash the montmorillonite suspension with deionized water to remove unreacted calcium salt and other impurities, and dry the washed montmorillonite to obtain the nano calcium-based montmorillonite.

[0063] Further, the specific steps of the step "adopting a pH-responsive microcapsule technology to mix the mixture with the dispersion liquid, and after coating and curing treatments, preparing the pet wound repair composition stone" are as follows:

[0064] Dissolve sodium polystyrene sulfonate and poly(lactic-co-glycolic acid) copolymer in an organic solvent to obtain an organic phase, drop the organic phase into deionized water, and stir and mix evenly. Remove the organic solvent in the system by evaporation to obtain a PLGA / PSS nanoparticle dispersion liquid;

[0065] Add the mixture to the PLGA / PSS nanoparticle dispersion liquid, mix and incubate for a period of time to load the drug onto the nanoparticles to obtain drug-loaded nanoparticles;

[0066] Drop the drug-loaded nanoparticles into the pectin solution, stir and mix, remove the supernatant by centrifugation, and then add deionized water to redisperse to obtain a pectin-coated drug-loaded nanoparticle dispersion liquid;

[0067] Add the pectin-coated drug-loaded nanoparticle dispersion liquid to the polyacrylic resin solution, stir and mix, add the mixed solution to the HCl solution, stir and mix to cure the microcapsules to obtain pH-responsive microcapsules containing drug powder;

[0068] After drying the pH-responsive microcapsules containing drug powder, prepare the pet wound repair composition stone.

[0069] Example 1:

[0070] The present invention provides a preparation method of a pet wound repair composition for preparing the pet wound repair composition as described above, which is characterized by including the following steps:

[0071] Prepare type II collagen by a bovine tendon enzymatic hydrolysis process;

[0072] The type II collagen is hydrolyzed by a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2000 Da;

[0073] The nano sodium montmorillonite is converted into the nano calcium montmorillonite by an ion exchange method;

[0074] Weigh 0.05% of the curcumin-phospholipid complex in the total amount of the pet wound repair composition and 5% of the nano krill oil microcapsule powder in the total amount of the pet wound repair composition, and mix them with the prepared hydrolyzed type II collagen and the nano calcium montmorillonite (the ratio of the hydrolyzed type II collagen to the nano calcium montmorillonite is 5:1) to obtain a mixture;

[0075] Using the pH-responsive microcapsule technology, the mixture is mixed with the dispersion liquid, and after coating and curing treatment, the pet wound repair composition is prepared.

[0076] Application Example 1: The prepared pet wound repair composition is used for testing in an osteoarthritis model of elderly cats;

[0077] The dosing regimen is as follows: The pet wound repair composition provided by this technical solution is orally administered at 0.5 g / kg per day (here is the ratio relationship between the weight of the elderly cat and the drug), for 28 consecutive days;

[0078] The dosing effect is as follows: Imaging evaluation shows that the joint space width increases by 0.23 mm (MRI T2 mapping shows that the cartilage water content decreases by 18%), and the synovial inflammation score drops from 3.2 to 1.7 (VAS evaluation standard).

[0079] Example 2:

[0080] The present invention provides a preparation method of a pet wound repair composition for preparing the pet wound repair composition as described above, which is characterized by including the following steps:

[0081] Prepare type II collagen by a bovine tendon enzymatic hydrolysis process;

[0082] The type II collagen is hydrolyzed by a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with active peptide segments having a molecular weight of 2400 Da;

[0083] The nano sodium montmorillonite is converted into the nano calcium montmorillonite by an ion exchange method;

[0084] Weigh 0.75% of the total amount of the pet wound repair composition of the curcumin-phospholipid complex and 10% of the total amount of the pet wound repair composition of the Antarctic krill oil microcapsule powder, and mix them with the prepared hydrolyzed type II collagen and the nano calcium montmorillonite (the ratio of the hydrolyzed type II collagen to the nano calcium montmorillonite is 3:1) to obtain a mixture;

[0085] Using the pH-responsive microcapsule technology, mix the mixture with the dispersion liquid, and after coating and curing treatments, prepare the pet wound repair composition.

[0086] Example 3:

[0087] The present invention provides a preparation method of a pet wound repair composition for preparing the pet wound repair composition as described above, which is characterized by including the following steps:

[0088] Prepare type II collagen by using a bovine tendon enzymatic hydrolysis process;

[0089] Hydrolyze type II collagen by using a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen with an active peptide segment having a molecular weight of 2800 Da;

[0090] Convert nano sodium montmorillonite into the nano calcium montmorillonite by using an ion exchange method;

[0091] Weigh 0.1% of the total amount of the pet wound repair composition of the curcumin-phospholipid complex and 15% of the total amount of the pet wound repair composition of the Antarctic krill oil microcapsule powder, and mix them with the prepared hydrolyzed type II collagen and the nano calcium montmorillonite (the ratio of the hydrolyzed type II collagen to the nano calcium montmorillonite is 1:1) to obtain a mixture;

[0092] Using the pH-responsive microcapsule technology, mix the mixture with the dispersion liquid, and after coating and curing treatments, prepare the pet wound repair composition.

[0093] The above-disclosed is only a preferred embodiment of the present invention. Of course, it cannot be used to limit the scope of the rights of the present invention. Those of ordinary skill in the art can understand all or part of the processes of implementing the above embodiments, and the equivalent changes made according to the claims of the present invention still fall within the scope covered by the invention.

Claims

1. A pet wound repair composition, characterized in that: The pet wound repair composition comprises hydrolyzed type II collagen, nano calcium-based montmorillonite, curcumin-phospholipid complex and Antarctic krill oil microcapsule powder, wherein the mass ratio of the hydrolyzed type II collagen to the nano calcium-based montmorillonite is 5:1 to 1:1, the curcumin-phospholipid complex accounts for 0.05-0.1% of the total amount of the pet wound repair composition, and the Antarctic krill oil microcapsule powder accounts for 5-15% of the total amount of the pet wound repair composition.

2. The pet wound repair composition according to claim 1, characterized in that: The molecular weight of the hydrolyzed type II collagen is 2000-3000Da.

3. The pet wound repair composition according to claim 2, characterized in that: The particle size of the nano calcium-based montmorillonite is ≤100nm and the specific surface area is ≥200m 2 / g.

4. A method for preparing a pet wound repair composition, for preparing the pet wound repair composition as claimed in claim 1, characterized in that: The steps include: Type II collagen was prepared by enzymatic hydrolysis of bovine Achilles tendon; The type II collagen is hydrolyzed by a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen having an active peptide segment with a molecular weight of 2000-2800 Da; The nano sodium-montmorillonite is converted into the nano calcium-montmorillonite by an ion exchange method; Weighing a certain amount of the curcumin-phospholipid complex and the Antarctic krill oil microcapsule powder, and mixing them with the prepared hydrolyzed type II collagen and the nano calcium-based montmorillonite to obtain a mixture; The mixture is mixed with the dispersion by using pH-responsive microcapsule technology, and after coating and curing, the pet wound repair composition is prepared.

5. The method for preparing the pet wound repair composition according to claim 4, characterized in that: The specific steps of the step "preparing type II collagen by enzymatic hydrolysis of bovine Achilles tendon" are: Select fresh or frozen beef Achilles tendon as raw material and pre-treat the raw material; The pretreated bovine Achilles tendon is washed and placed in a container containing ethanol for soaking; Put the pre-treated bovine Achilles tendon into an enzymatic hydrolysis tank, add enzymatic hydrolysis solution, ensure that the bovine Achilles tendon is completely immersed in the enzymatic hydrolysis solution, and perform enzymatic hydrolysis reaction to release collagen; After the enzymatic hydrolysis is completed, the collagen in the enzymatic hydrolysis solution is extracted and purified to obtain type II collagen.

6. The method for preparing the pet wound repair composition according to claim 5, characterized in that: The specific steps of the step "hydrolyzing type II collagen using a composite enzymatic hydrolysis process to obtain the hydrolyzed type II collagen having an active peptide segment with a molecular weight of 2000-2800 Da" are: Pre-treating type II collagen, and adding the pre-treated type II collagen into an enzymatic hydrolysis container; Add trypsin to the enzymatic hydrolysis container, and after the reaction is complete, add bromelain to achieve step-by-step enzymatic hydrolysis; After the enzymatic hydrolysis is completed, the active peptide segments with a molecular weight of 2000-2800Da are separated from the reaction system by separation and purification technology, thereby obtaining the hydrolyzed type II collagen with an active peptide segment with a molecular weight of 2000-2800Da.

7. The method for preparing the pet wound repairing composition according to claim 6, characterized in that: In the step of "adding trypsin to the enzymatic hydrolysis container, and after the reaction is completed, adding bromelain to achieve step-by-step enzymatic hydrolysis", the degree of hydrolysis DH needs to be determined by monitoring the pH value change and nitrogen release of the reaction system to ensure that it is within the range of 18-22%.

8. The method for preparing the pet wound repairing composition according to claim 7, characterized in that: The specific steps of the step "using ion exchange method to convert the nano sodium-based montmorillonite into the nano calcium-based montmorillonite" are: Dispersing nano sodium montmorillonite in an appropriate amount of deionized water to form a uniform suspension; Slowly adding the pretreated montmorillonite suspension into the calcium salt solution to carry out an ion exchange reaction; After the reaction is completed, the montmorillonite suspension is washed with deionized water to remove unreacted calcium salt and other impurities, and the washed montmorillonite is dried to obtain the nano calcium-based montmorillonite.

9. The method for preparing the pet wound repair composition according to claim 8, characterized in that: The specific steps of the step "adopting pH-responsive microcapsule technology to mix the mixture with the dispersion, and after coating and curing treatment, preparing the pet wound repair composition stone" are: Sodium polystyrene sulfonate and poly(lactic acid-co-glycolic acid) are dissolved in an organic solvent to obtain an organic phase, the organic phase is added dropwise into deionized water, and stirred and mixed uniformly, and the organic solvent in the system is removed by evaporation to obtain a PLGA / PSS nanoparticle dispersion; The mixture is added to the PLGA / PSS nanoparticle dispersion, mixed and incubated for a period of time to load the drug onto the nanoparticles, thereby obtaining drug-loaded nanoparticles; The drug-loaded nanoparticles are added dropwise to the pectin solution, stirred and mixed, the supernatant is removed by centrifugation, and deionized water is added to redisperse to obtain a dispersion of pectin-coated drug-loaded nanoparticles; Adding a dispersion of pectin-coated drug-loaded nanoparticles to a polyacrylic acid resin solution, stirring and mixing, adding the mixed solution to an HCl solution, stirring and mixing, and solidifying the microcapsules to obtain pH-responsive microcapsules containing drug powder; The pH-responsive microcapsules containing the drug powder are dried to prepare the pet wound repairing composition stone.

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