Composition for scavenging free radicals and relieving visual fatigue and application

Abstract

The invention relates to the technical field of food, and discloses a composition for scavenging free radicals and relieving visual fatigue and application, the composition comprises the following components: 50-200 parts of blueberry fruit powder, 50-200 parts of blackcurrant fruit powder, 50-200 parts of black wolfberry fruit powder, 4-12 parts of lutein ester microcapsule powder, 1-4 parts of zeaxanthin, 50-200 parts of blueberry anthocyanin, 30-200 parts of DHA algal oil microcapsule powder and 5-20 parts of pyrroloquinoline quinone. The composition is a functional food, a health food or a medicine for scavenging free radicals, relieving visual fatigue, and preventing and assisting in treating vision decline caused by maculopathy and retinitis pigmentosa. Natural plant components and an efficient antioxidant are scientifically proportioned, the stability and bioavailability of the components are improved by adopting a microencapsulation technology, free radicals are effectively removed, visual fatigue is relieved, comprehensive nutrients needed by eyes are provided, and possible side effects in traditional eye protection products are avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of food, and specifically to a composition for scavenging free radicals and relieving visual fatigue and its applications. Background Art

[0002] With the rapid development of modern society and the popularization of information technology, especially the widespread use of electronic devices, visual fatigue has become an increasingly serious problem globally. Visual fatigue is usually caused by excessive eye use and is manifested as symptoms such as blurred vision, eye soreness, dryness, and tearing. The occurrence of visual fatigue is closely related to multiple factors, such as long eye use time, improper eye use distance, poor lighting conditions, mismatched eyeglass diopters, and lack of sufficient outdoor activities. Long-term eye fixation and continuous metabolism of extraocular muscles and ciliary muscles cause the continuous accumulation of metabolic wastes in the eyes (such as free radicals), which in turn leads to damage and functional decline of eye cells.

[0003] In recent years, due to the accelerated work rhythm and the popularization of electronic screens, the visual burden of modern people has been increasing. Especially long-term and close contact with electronic products is likely to cause visual fatigue, resulting in symptoms such as eye soreness and vision decline. Related visual problems have also gradually increased, including macular lesions, retinitis pigmentosa, glaucoma, cataract, etc. These problems not only affect visual health but may even lead to permanent vision loss in severe cases.

[0004] In daily life, free radicals are the main damaging factors caused by long-term eye use or the external environment (such as blue light emitted by electronic devices). Free radicals can damage the retina and lens of the eyes, resulting in reduced elasticity of the lens and blurred retinal imaging, thus causing eye diseases such as myopia. Especially the blue light emitted by electronic devices will generate a large amount of reactive oxygen species (ROS) after entering the eyes. These free radicals cause serious damage to the macula and may even cause macular lesions and blindness.

[0005] Currently, there are already some functional foods and health products on the market attempting to solve these problems, especially for relieving visual fatigue and protecting eye health. However, most of the existing technologies are still limited to the use of single components. For example, lutein is used to filter blue light, or antioxidant components such as vitamin C are relied on to slow down the effects of free radicals. Although these components can provide protection to a certain extent, their effects are often limited and do not fully comprehensively regulate the multiple causes of free radical accumulation and visual fatigue.

[0006] In the existing technology, although some products relieve visual fatigue through single antioxidant ingredients such as lutein, vitamin E, etc., they lack a comprehensive solution of multi-ingredient synergistic effect, which makes them unable to solve the problem of cell damage caused by free radicals, especially in the case of long-term accumulation of visual fatigue, the effect is not long-lasting and comprehensive. Existing products usually rely on single nutritional supplements or antioxidants, without effectively utilizing the synergistic effect of natural ingredients. Summary of the invention

[0007] In view of the deficiencies of the prior art, the present invention provides a composition and application for scavenging free radicals and relieving visual fatigue, which solves the problems of insufficient free radical scavenging and unsustainable visual fatigue relief effect in the prior art.

[0008] To achieve the above objectives, the present invention is implemented through the following technical scheme: a composition for scavenging free radicals and relieving visual fatigue, comprising the following components by weight: 50-200 parts of blueberry fruit powder, 50-200 parts of black currant fruit powder, 50-200 parts of black wolfberry powder, 4-12 parts of lutein ester microcapsule powder, 1-4 parts of zeaxanthin, 50-200 parts of blueberry anthocyanins, 30-200 parts of DHA algae oil microcapsule powder and 5-20 parts of pyrroloquinoline quinone.

[0009] Preferably, the blackcurrant fruit powder is blackcurrant powder rich in anthocyanins and polyphenols.

[0010] Preferably, the lutein ester microcapsule powder is a combination of lutein and fatty acids.

[0011] A method for preparing a composition for removing free radicals and relieving visual fatigue comprises the following steps:

[0012] S1. Mix blueberry powder, blackcurrant powder, black wolfberry powder, lutein ester microcapsule powder, zeaxanthin, blueberry anthocyanin, DHA algae oil microcapsule powder and pyrroloquinoline quinone according to the mass proportions;

[0013] S2, drying the mixture, wherein the drying temperature is controlled between 4°C and 60°C, and the drying time is 2-4h;

[0014] S3, crushing the dried mixture to a particle size of 100-300 μm;

[0015] S4, uniformly mixing the crushed mixture;

[0016] S5. Add appropriate amount of auxiliary materials to prepare the preparation

[0017] Preferably, the adjuvants include one or more of food additives, acidulants, sweeteners, bleaching agents, food pigments, hardeners, preservatives, bactericides, antioxidants, thickeners, emulsifiers, lubricants, fillers, binders, fortifiers, effervescent agents, and disintegrants.

[0018] Preferably, the preparation is in the form of powder, tablet, capsule, granule, oral preparation or dripping pill.

[0019] Use of a composition for scavenging free radicals and relieving visual fatigue, the composition being composed of the ingredients as described above, and the composition being used for functional foods, health foods or drugs for scavenging free radicals, relieving visual fatigue, preventing and adjuvantly treating visual decline caused by macular lesions and retinitis pigmentosa.

[0020] The present invention provides a composition for scavenging free radicals and relieving visual fatigue and its application. It has the following beneficial effects:

[0021] 1. The composition of the present invention combines natural plant components such as lutein, zeaxanthin, and blueberry anthocyanins, especially lutein and zeaxanthin. These components are not only the main pigments in the macular area of the retina but also essential nutrients required by the human body. By selecting these natural and edible components and using microencapsulation technology to improve the stability and bioavailability of the components, the technical effect of non-toxic side effects is achieved. Compared with some existing products that rely on synthetic chemical components or high doses of single components, the present invention, through scientific formulation and selection of natural raw materials, avoids possible toxic side effects, ensures the safety of long-term use, and effectively solves the problem of possible side effects in traditional eye care products.

[0022] 2. The present invention is based on natural plant components such as blueberry fruit powder, blackcurrant fruit powder, and black wolfberry powder, and combines various eye care components such as lutein ester, zeaxanthin, and DHA algal oil microcapsule powder. In particular, the addition of pyrroloquinoline quinone, a component that efficiently scavenges free radicals, achieves the technical effect of synergistic enhancement. Compared with the technical solutions in the prior art that are mostly single components or only rely on conventional antioxidants, through scientific material selection and formulation, while each component exerts its individual efficacy, a powerful synergistic effect is formed, significantly enhancing the effects in multiple aspects such as scavenging free radicals, improving ocular microcirculation, and relieving visual fatigue. In particular, the combination of PQQ as a powerful antioxidant with other natural components can not only quickly scavenge free radicals but also play an active role in promoting the repair of retinal cells and maintaining ocular health, thus effectively solving the problem of single components or limited effects in some existing products. Description of the Drawings

[0023] Figure 1 It is a flow chart of the preparation method of the composition of the present invention. Detailed Embodiments

[0024] Next, in conjunction with the accompanying drawings of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0025] Please refer to the attached Figure 1 , the embodiment of the present invention provides a composition for scavenging free radicals and relieving visual fatigue, which includes the following components by weight: 50-200 parts of blueberry fruit powder, 50-200 parts of blackcurrant fruit powder, 50-200 parts of black wolfberry powder, 4-12 parts of lutein ester microcapsule powder, 1-4 parts of zeaxanthin, 50-200 parts of blueberry anthocyanin, 30-200 parts of DHA algal oil microcapsule powder, and 5-20 parts of pyrroloquinoline quinone.

[0026] The blackcurrant fruit powder is a powder of blackcurrant rich in anthocyanins and polyphenols.

[0027] The lutein ester microcapsule powder is a conjugate of lutein and fatty acids.

[0028] Specifically, the blueberry fruit powder is made from blueberries, which are rich in anthocyanins. It is a powerful antioxidant that can effectively scavenge free radicals. The accumulation of free radicals will damage eye cells, especially the retina and lens, leading to vision problems. Anthocyanins reduce oxidative stress through antioxidant effects, prevent retinal damage, and improve the microvascular circulation of the eyes, thereby relieving visual fatigue and improving visual acuity. Vitamin C in blueberries also helps strengthen the immune function and protect the eyes from damage by the external environment.

[0029] The blackcurrant fruit powder is made from blackcurrants, which are rich in anthocyanins and polyphenols. They have powerful antioxidant and anti-inflammatory effects and can significantly reduce oxidative damage caused by long-term eye use or screen radiation. These components can enhance the blood flow in the microvessels of the eyes and improve the absorption ability of the retina for nutrients, thereby promoting eye health. Vitamin C and vitamin E in blackcurrants can also enhance immunity and further help eye cells resist oxidative stress.

[0030] The black wolfberry powder is made from black wolfberries, which contain rich wolfberry polysaccharides, amino acids, vitamins, and minerals. It can effectively improve blood circulation and increase the blood flow in the microvessels of the eyes, thereby enhancing the metabolic and repair abilities of retinal cells. In addition, the antioxidant components in black wolfberries can protect the eyes from oxidative stress caused by external factors such as electronic devices or ultraviolet rays and slow down eye aging.

[0031] Lutein in lutein ester microcapsule powder is a carotenoid mainly present in the macular area of the eye, with the functions of absorbing blue light and antioxidation. It can effectively filter blue light, reduce the damage of blue light to the macular area, and prevent macular lesions. Lutein ester is a conjugate of lutein and fatty acid, with good bioavailability and can be converted into lutein in the body. By protecting the retina and enhancing the antioxidant capacity of the eyes, lutein can relieve visual fatigue and reduce the burden on the eyes.

[0032] Zeaxanthin and lutein together constitute the macular pigment in the macular area of the retina, and its concentration is relatively high in the fovea centralis of the eye. Zeaxanthin has strong antioxidant capacity and can effectively counteract free radical damage caused by blue light, ultraviolet light and pollution. It can also enhance the acuity of vision, help improve visual performance in low-light environments, and prevent eye diseases caused by free radicals, such as age-related macular degeneration.

[0033] Blueberry anthocyanins are a class of strong water-soluble antioxidants, which can protect the eyes from oxidative damage by scavenging free radicals and increasing the activity of antioxidant enzymes. It can enhance the antioxidant capacity of retinal and lens cells and prevent damage to the retina caused by long-term exposure to screen radiation and ultraviolet light. In addition, blueberry anthocyanins can also improve ocular microcirculation and help the eyes recover from fatigue.

[0034] DHA in DHA algal oil microcapsule powder is an important essential fatty acid, which is widely distributed in the retina. It is crucial for maintaining retinal health, improving visual acuity and supporting visual development. DHA from algal oil helps to promote the formation of retinal pigment molecules and enhance the eye's adaptability to light. By increasing the antioxidant capacity of the retina, DHA can reduce the accumulation of free radicals, thereby protecting the eyes from aging and vision decline.

[0035] Pyrroloquinoline quinone (PQQ) is a strong antioxidant that can directly scavenge free radicals in the body, especially various reactive oxygen species (ROS). By reducing oxidative stress, PQQ protects eye tissues from oxidative damage and prevents cell aging of the retina and lens. PQQ can also enhance the metabolic capacity of cells by promoting mitochondrial function, improve the repair and regeneration ability of eye cells, and help maintain normal visual function.

[0036] A preparation method of a composition for scavenging free radicals and relieving visual fatigue, comprising the following steps:

[0037] S1. Mix blueberry fruit powder, blackcurrant fruit powder, black wolfberry powder, lutein ester microcapsule powder, zeaxanthin, blueberry anthocyanins, DHA algal oil microcapsule powder and pyrroloquinoline quinone according to mass parts;

[0038] S2. Dry the above mixture, control the drying temperature between 4°C and 60°C, and the drying time is 2 - 4 h;

[0039] S3. Crush the dried mixture, and control the crushing particle size within 100 - 300 μm;

[0040] S4. Uniformly mix the crushed mixture;

[0041] S5. Add appropriate excipients and process them into preparations.

[0042] The excipients include one or two or more of food additives, acidulants, sweeteners, bleaching agents, food pigments, hardeners, preservatives, bactericides, antioxidants, thickeners, emulsifiers, lubricants, fillers, binders, fortifiers, effervescent agents, and disintegrants.

[0043] The preparation is powder, tablet, capsule, granule, oral preparation or dripping pill.

[0044] Application of a composition for scavenging free radicals and relieving visual fatigue. The composition is composed of the following ingredients. The composition is used for scavenging free radicals, relieving visual fatigue, and is a functional food, health food or medicine for preventing and adjuvantly treating vision decline caused by macular lesions and retinitis pigmentosa.

[0045] Example 1: Preparation of tablets

[0046] In this example, we will show how to prepare a tablet of a composition for scavenging free radicals and relieving visual fatigue according to the technical solution of the present invention. Through the following specific steps, the problem of visual fatigue proposed in the background technology is solved, and an effective relief effect is achieved, especially in terms of vision decline and eye discomfort caused by long-term eye use.

[0047] Step 1: Weigh the raw materials

[0048] First, prepare the basic raw materials of the composition and weigh them according to the following ratio:

[0049] 200 parts of blueberry fruit powder;

[0050] 100 parts of blackcurrant fruit powder;

[0051] 50 parts of black wolfberry powder;

[0052] 6 parts of lutein ester microcapsule powder;

[0053] 2 parts of zeaxanthin;

[0054] 100 parts of blueberry anthocyanins;

[0055] 50 parts of DHA algal oil microcapsule powder;

[0056] 10 parts of pyrroloquinoline quinone (PQQ).

[0057] In addition, as an excipient, 28.2 g of maltodextrin was prepared to improve the taste of the tablets and the flowability of the powder.

[0058] Step 2: Mix the raw materials

[0059] Mix the above raw materials with the excipient (maltodextrin) to ensure uniform distribution of the raw materials. To ensure the mixing effect, pass the mixture through an 80-mesh sieve to remove larger particles and ensure uniformity.

[0060] Step 3: Drying

[0061] Put the mixed materials into an oven for constant-temperature drying. Set the temperature at 50 °C and maintain this temperature for 4 hours to ensure that the moisture in the raw materials is fully evaporated and avoid the influence of moisture on the stability and preservation of the tablets. After drying, use an ultraviolet lamp for sterilization for 30 minutes to ensure the hygienic safety of the product.

[0062] Step 4: Crushing and tableting

[0063] The dried materials are crushed by a crusher, and the target particle size is 100 - 300 μm to ensure fine powder and easy tableting. Next, the crushed materials are tableted by a tableting machine. The weight of each tablet is controlled at 0.8 g, and finally 100 tablets are obtained.

[0064] Free radical scavenging ability test experiment:

[0065] To verify the effect of the composition of the present invention in scavenging free radicals, we used the DPPH free radical scavenging method and the ABTS cation free radical scavenging method for experiments. All experiments were carried out under standardized conditions, and the results showed that the composition of the present invention could significantly scavenge free radicals, and the effect was better than that of ascorbic acid as a positive control.

[0066] Experimental method:

[0067] Determination of DPPH free radical scavenging ability:

[0068] First, prepare a 0.5 mmol / L DPPH solution using 95% ethanol as a solvent. Under the condition of 37 °C, mix 100 μL of the DPPH solution with 100 μL of the sample solution and incubate in the dark for 30 minutes. Then, measure the absorbance at 517 nm and record it as A1. At the same time, ultrapure water is used as a blank control, and the absorbance is recorded as A0. Then, replace the DPPH solution with ultrapure water and mix it with 100 μL of the sample solution. After incubating for 30 minutes, measure the absorbance and record it as A2. Calculate the DPPH scavenging rate through the formula:

[0069]

[0070] Determination of the scavenging ability of ABTS cation radicals:

[0071] Prepare a 0.1 mmol / L ABTS cation working solution with an absorbance stable between 0.7 - 0.8 at 734 nm. Mix 100 μL of the sample solution with 100 μL of the ABTS cation working solution, measure the absorbance at 734 nm, and record it as A1. Use ultrapure water to replace the sample solution as a control, and record it as A0; replace the ABTS cation working solution with ultrapure water and mix it with 100 μL of the sample solution, measure the absorbance after incubating in the dark at 37 °C for 30 minutes, and record it as A2. Calculate the ABTS cation scavenging rate according to the following formula:

[0072]

[0073] Table 2:

[0074] Group DPPH Scavenging Rate (%) ABTS Cation Scavenging Rate (%) Ascorbic Acid 50.3±3.5 38.6±4.6 Example 1 77.4±4.2 66.2±4.2

[0075] Summary: As can be seen from Table 1, the composition of the present invention is significantly superior to ascorbic acid in terms of the scavenging ability of DPPH and ABTS cation radicals. The DPPH scavenging rate increased by 27.1 percentage points, and the ABTS cation scavenging rate increased by 27.6 percentage points. This indicates that the composition of the present invention has a good effect in scavenging free radicals, can effectively reduce cell damage caused by free radicals, and has a strong antioxidant effect.

[0076] Human trial:

[0077] In order to further evaluate the actual effect of the composition of the present invention in relieving visual fatigue and improving eye health, we conducted a human trial. According to the "Technical Specification for the Inspection and Evaluation of Health Foods" (2023 Edition), a comparative trial was carried out between the trial group and the control group to observe the improvement of eye discomfort symptoms.

[0078] Experimental method:

[0079] In this human trial, 100 participants were randomly selected and divided into a trial group and a control group. The trial group took the tablets in Example 1 of the present invention orally every day for 4 weeks. Before and after the trial, the symptoms of eye soreness, eye swelling, photophobia, blurred vision, dry eyes, etc. of the participants were recorded and analyzed, and the changes between the trial group and the control group were compared. The clinical symptom scoring method was used for evaluation.

[0080] Table 2:

[0081] Symptom Control Group Test Group Improvement Rate (%) of Control Group Improvement Rate (%) of Test Group Eye Soreness 47 28 6 56 Eye Swelling 46 20 8 39.2 Photophobia 48 18 7.7 36 Blurred Vision 47 24 9.6 46.2 Dry Eye 48 20 5.9 40 Foreign Body Sensation 48 20 24 40 Tearing 43 8 14 16 General Discomfort 46 19 16 37.3

[0082] Summary: According to the test results, the improvements in multiple symptoms of the test group were significantly higher than those of the control group, and the effective rate was significantly increased. The eye symptom scores of the test group decreased significantly, the duration of clear vision improved, and there was no significant difference in visual persistence compared with the control group (P>0.05), but it performed well in relieving visual fatigue and symptom improvement.

[0083] Conclusion: It can be seen from the results of the human feeding test that the composition of the present invention has the function of relieving visual fatigue. By effectively scavenging free radicals and reducing oxidative damage, it significantly improves the symptoms related to eye fatigue, indicating that the composition has the potential in preventing and relieving vision problems, especially suitable for relieving the discomfort symptoms caused by long-term eye use.

[0084] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composition for scavenging free radicals and relieving visual fatigue, characterized in that: The invention comprises the following components by weight: 50-200 parts of blueberry powder, 50-200 parts of blackcurrant powder, 50-200 parts of black wolfberry powder, 4-12 parts of lutein ester microcapsule powder, 1-4 parts of zeaxanthin, 50-200 parts of blueberry anthocyanin, 30-200 parts of DHA algae oil microcapsule powder and 5-20 parts of pyrroloquinoline quinone.

2. A composition for scavenging free radicals and relieving visual fatigue according to claim 1, characterized in that: The blackcurrant fruit powder is blackcurrant powder rich in anthocyanins and polyphenols.

3. A composition for scavenging free radicals and relieving visual fatigue according to claim 1, characterized in that: The lutein ester microcapsule powder is a combination of lutein and fatty acids.

4. A method for preparing a composition for scavenging free radicals and relieving visual fatigue, characterized in that: The following steps are involved: S1. Mix blueberry powder, blackcurrant powder, black wolfberry powder, lutein ester microcapsule powder, zeaxanthin, blueberry anthocyanin, DHA algae oil microcapsule powder and pyrroloquinoline quinone according to the mass proportions; S2, drying the mixture, wherein the drying temperature is controlled between 4°C and 60°C, and the drying time is 2-4h; S3, crushing the dried mixture to a particle size of 100-300 μm; S4, uniformly mixing the crushed mixture; S5. Add appropriate amount of auxiliary materials to prepare the preparation.

5. The method for preparing a composition for scavenging free radicals and relieving visual fatigue according to claim 4, characterized in that: The auxiliary materials include one or two or more of food additives, acidulants, sweeteners, bleaching agents, food pigments, hardeners, preservatives, bactericides, antioxidants, thickeners, emulsifiers, lubricants, fillers, adhesives, fortifiers, effervescent agents, and disintegrants.

6. The method for preparing a composition for scavenging free radicals and relieving visual fatigue according to claim 4, characterized in that: The preparation is in the form of powder, tablet, capsule, granule, oral preparation or drop pill.

7. Use of a composition for removing free radicals and relieving visual fatigue, wherein the composition is composed of the ingredients according to claim 1, characterized in that: The composition is a functional food, health food or medicine for removing free radicals, relieving visual fatigue, preventing and assisting in the treatment of visual atrophy caused by macular degeneration and retinal pigment degeneration.

Images