Vegetable oil essential oil composition and application

Abstract

The invention belongs to the technical field of daily chemicals, and discloses a vegetable oil and essential oil composition and application thereof. The plant oil and essential oil composition is prepared from the following components in parts by weight: 50 to 80 parts of crambe abyssinica seed oil, 5 to 20 parts of rosa rugosa seed oil, 8 to 15 parts of evening primrose oil, 2 to 15 parts of acanthopanax gracilistylus root and bark extract and 1.2 to 5 parts of jasminum grandiflorum extract. The vegetable oil and essential oil composition is added into massage oil and can go deep into the bottom layer of skin, inhibit generation and release of inflammatory factors, relieve inflammatory response of the skin, relieve symptoms such as red and swollen, pain and itching caused by inflammation, balance grease secretion of the skin, promote blood circulation of the skin and relieve inflammation. The elasticity and luster of the skin are improved, the formation of stains and darkness is reduced, and the skin is brighter and more uniform.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and particularly relates to a vegetable oil essential oil composition and its application. Background Art

[0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants by steam distillation, pressing, cold extraction or solvent extraction methods. Essential oils are composed of many different molecules, and the structures and properties of these molecules determine the aroma, efficacy and permeability of essential oils. For example, rose essential oil can be composed of more than 250 different molecules combined together, which gives it a unique aroma and efficacy. Essential oils are lipophilic, easily soluble in oils, and their molecular chains are short, enabling them to quickly penetrate the skin and enter the blood circulation system through the capillaries in subcutaneous fat, exerting various effects such as antibacterial, anti-inflammatory and antioxidant effects, and thus maintaining physical health. In addition, the small molecules of essential oils can also be absorbed into the body through the nasal mucosa tissue and directly affect the limbic system of the brain, thereby regulating emotions and the physiological functions of the body. For example, in aromatherapy, essential oils are widely used to enhance physiological and psychological functions. Using essential oils by inhalation, massage or bathing can help people relieve stress, improve sleep, enhance concentration, relieve pain, etc.

[0003] With the improvement of living standards, people's demand for essential oil products is increasing continuously. Especially when facing health problems such as skin care, emotion management, respiratory system maintenance and daily body care, people have a strong demand for high-efficiency, safe and natural essential oil products. However, traditional essential oil products mostly rely on chemical compounds, which not only weaken the natural advantages of essential oils and cannot exert the true efficacy of essential oils, but also easily pose potential threats to human health. Long-term use of these products can cause adverse effects such as skin allergies, damaged skin barriers, and liver and kidney function damage. In addition, the current existing essential oils have a single efficacy, and people often need to purchase multiple essential oils to meet various needs. Therefore, there is an urgent need to develop a safer and more efficient essential oil that can achieve multiple effects, meet the market demand for high-quality health products, and promote the sustainable development of the essential oil industry. Summary of the Invention

[0004] The purpose of the present invention is to provide a vegetable oil essential oil composition and its application by overcoming the deficiencies of the prior art.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] In a first aspect, the present invention provides a vegetable oil essential oil composition, comprising the following components in parts by weight: 50-80 parts of Crambe abyssinica seed oil, 5-20 parts of Rosa rubiginosa seed oil, 8-15 parts of evening primrose oil, 2-15 parts of Acanthopanax gracilistylus root bark extract, and 1.2-5 parts of Jasminum grandiflorum extract.

[0007] Through the synergistic effect of various active ingredients such as Crambe abyssinica seed oil, Rosa rubiginosa seed oil, evening primrose oil, Acanthopanax gracilistylus root bark extract, and Jasminum grandiflorum extract, the vegetable oil essential oil composition of the present invention achieves remarkable anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects. Moreover, its active ingredients are all natural vegetable oils or vegetable oil extracts, avoiding the use of chemical compounds and reducing the risk of irritation and side effects on the skin and body. It is suitable for various needs such as mood management, pain relief, strengthening the tendons, promoting blood circulation to remove blood stasis, and skin care, with good safety and mildness. Among them, Crambe abyssinica seed oil is rich in long-chain fatty acid C22, which can cooperate with other components to improve and enhance the antioxidant property and natural stability of the skin on the one hand, prevent the invasion of external harmful substances to the skin, and on the other hand, make the composition have excellent skin affinity, and can easily penetrate into the deep layer of the skin to provide necessary nutrition and moisture for the skin; Rosa rubiginosa seed oil cooperates with other components to effectively play the roles of deep moisturizing, antioxidant, and anti-aging, and improve skin health; in addition to palmitic acid, oleic acid, and stearic acid, evening primrose oil also contains about 70% linoleic acid and about 9% γ-linolenic acid. Among them, γ-linolenic acid is one of the polyunsaturated fatty acids required by the human body and can be converted into arachidonic acid in the body to provide precursors for the formation of prostaglandins and biological membranes; Acanthopanax gracilistylus root bark extract is rich in active substances such as polysaccharides, isofraxidin, chlorogenic acid, sesamin, stearic acid, and β-sitosterol. Furthermore, evening primrose oil can be compounded with Acanthopanax gracilistylus root bark extract to further cooperate with other components to inhibit the production and release of inflammatory mediators, regulate the inflammatory response, scavenge free radicals, protect the skin from oxidative damage, and effectively play the roles of enhancing immunity, anti-inflammatory, soothing, and antioxidant; Jasminum grandiflorum extract cooperates with other components to jointly resist the invasion of free radicals, slow down the aging process, reduce skin inflammation, promote skin repair, and effectively play the roles of anti-inflammatory, soothing, and antioxidant.

[0008] Therefore, under the synergistic action of multiple components, the vegetable oil essential oil composition of the present invention can penetrate to the bottom layer of the skin to inhibit the production and release of inflammatory factors, reduce the inflammatory response of the skin, and soothe symptoms such as redness, pain, and itching caused by inflammation, promoting the repair and regeneration of the skin; at the same time, they can also balance the sebum secretion of the skin, relieve skin sensitivity and discomfort, promote blood circulation in the skin, and promote the synthesis of collagen and the formation of elastic fibers in the skin, reducing the formation of fine lines and wrinkles and increasing the elasticity and luster of the skin; in addition, they can scavenge free radicals, protect the skin from oxidative damage, delay the aging process of the skin, reduce the formation of age spots and dullness, and make the skin brighter and more uniform. In summary, by reasonably compounding multiple active ingredients, the present invention not only successfully constructs a powerful and multi-functional essential oil system, but also effectively improves the barrier function and health status of the skin.

[0009] As a preferred embodiment of the vegetable oil essential oil composition of the present invention, the vegetable oil essential oil composition comprises the following components in parts by weight: 56 - 78.8 parts of Crambe abyssinica seed oil, 9 - 17 parts of Rosa rubiginosa seed oil, 8 - 15 parts of evening primrose oil, 3 - 8 parts of Acanthopanax gracilistylus root bark extract, and 1.2 - 4 parts of Jasminum grandiflorum extract.

[0010] Preferably, the vegetable oil essential oil composition comprises the following components in parts by weight: 67.14 parts of Crambe abyssinica seed oil, 13.16 parts of Rosa rubiginosa seed oil, 11.28 parts of evening primrose oil, 5.64 parts of Acanthopanax gracilistylus root bark extract, and 2.78 parts of Jasminum grandiflorum extract.

[0011] As a preferred embodiment of the vegetable oil essential oil composition of the present invention, the mass ratio of the evening primrose oil to the Acanthopanax gracilistylus root bark extract is 1:(0.15 - 1.5).

[0012] Preferably, the mass ratio of the evening primrose oil to the Acanthopanax gracilistylus root bark extract is 1:(0.375 - 0.75).

[0013] More preferably, the mass ratio of the evening primrose oil to the Acanthopanax gracilistylus root bark extract is any one or both of the range values of 1:0.375, 1:0.4, 1:0.425, 1:0.45, 1:0.475, 1:0.5, 1:0.525, 1:0.55, 1:0.575, 1:0.6, 1:0.65, 1:0.7, 1:0.75.

[0014] In a second aspect, the present invention provides a preparation method of the vegetable oil essential oil composition, comprising the following steps: mixing the Crambe abyssinica seed oil, Rosa rubiginosa seed oil, evening primrose oil, Acanthopanax gracilistylus root bark extract, and Jasminum grandiflorum extract evenly, and that's it.

[0015] In a third aspect, the present invention provides a massage oil, which comprises the vegetable oil essential oil composition described above.

[0016] By reasonably proportioning the vegetable oil essential oil composition of the present invention with other auxiliaries, the massage oil of the present invention provides a massage oil that can safely and efficiently achieve the effects of anti-inflammation, soothing, anti-wrinkle, and anti-oxidation, and has good safety and mildness.

[0017] As a preferred embodiment of the massage oil of the present invention, the massage oil comprises the following components in weight percentages: 9.5%-18% of the vegetable oil essential oil composition, 60%-75% of the base oil, 5%-10% of the blending oil, 1%-2.5% of the conditioner, 0.2%-1% of the antioxidant, 0.05%-0.3% of the soother, 0.03%-0.1% of the heat-generating agent, and the balance of the solvent.

[0018] Preferably, the massage oil comprises the following components in weight percentages: 9.5%-18% of the vegetable oil essential oil composition, 66%-69% of the base oil, 5.98%-8.35% of the blending oil, 1.61%-2.05% of the conditioner, 0.2%-0.8% of the antioxidant, 0.1%-0.27% of the soother, 0.03%-0.1% of the heat-generating agent, and the balance of the solvent.

[0019] More preferably, the massage oil comprises the following components in weight percentages: 13.3% of the vegetable oil essential oil composition, 67.17% of the base oil, 6.24% of the blending oil, 1.77% of the conditioner, 0.46% of the antioxidant, 0.18% of the soother, 0.06% of the heat-generating agent, and the balance of the solvent.

[0020] As a preferred embodiment of the massage oil of the present invention, the base oil comprises at least one of sunflower seed oil, olive fruit oil, European hazelnut oil, and macadamia nut oil; the blending oil comprises at least one of lemon peel oil, mugwort leaf oil, Michelia alba leaf oil, bergamot fruit oil, ginger root oil, Angelica archangelica root oil, Ligusticum wallichii oil, and Pelargonium graveolens oil; the conditioner comprises at least one of the extracts of Clerodendrum cyrtophyllum Turcz., Spatholobus suberectus Dunn, Speranskia tuberculata (Bge.) Baill., and Panax notoginseng root; the soother comprises the extract of Glycyrrhiza glabra L. and / or stearyl glycyrrhetinate. Preferably, the base oil comprises sunflower seed oil, olive fruit oil, European hazelnut oil, and macadamia nut oil; the blending oil comprises lemon peel oil, mugwort leaf oil, Michelia alba leaf oil, bergamot fruit oil, ginger root oil, Angelica archangelica root oil, Ligusticum wallichii oil, and Pelargonium graveolens oil; the conditioner comprises the extracts of Clerodendrum cyrtophyllum Turcz., Spatholobus suberectus Dunn, Speranskia tuberculata (Bge.) Baill., and Panax notoginseng root; the soother comprises the extract of Glycyrrhiza glabra L. and stearyl glycyrrhetinate.

[0021] As a preferred embodiment of the massage oil of the present invention, the antioxidant includes tocopheryl acetate; the heat-sensing agent includes vanillyl butyl ether, and the solvent includes squalane.

[0022] Fourthly, the present invention provides a preparation method of the massage oil, comprising the following steps:

[0023] (1) Mix the vegetable oil essential oil composition, the base oil, and the solvent uniformly to obtain mixture A;

[0024] (2) Mix the blended oil and the conditioner uniformly to obtain mixture B;

[0025] (3) Mix the antioxidant, the soothing agent, and the heat-sensing agent uniformly to obtain mixture C;

[0026] (4) Mix mixture A, mixture B, and mixture C, stir, and let stand to obtain the finished product.

[0027] The preparation method of the massage oil of the present invention has mild reaction conditions, simple operation steps, and is easy to scale up for industrial production.

[0028] As a preferred embodiment of the preparation method of the massage oil of the present invention, the stirring time is 2h - 5h; the standing time is 10h - 15h.

[0029] Fifthly, the present invention provides the application of the vegetable oil essential oil composition and the massage oil in skin care products and health care products.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows: First, through the synergistic effect of various active ingredients, the vegetable oil essential oil composition of the present invention achieves significant anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects, and its active ingredients are all natural vegetable oils or vegetable oil extracts, avoiding the use of chemical synthetic substances, reducing the risk of irritation and side effects on the skin and body, and having good safety and mildness for various needs such as mood management, pain relief, strengthening the tendons, promoting blood circulation to remove blood stasis, and skin care. Secondly, the massage oil prepared by adding the vegetable oil essential oil composition of the present invention into the massage oil can penetrate deep into the bottom layer of the skin, inhibit the production and release of inflammatory factors, reduce the inflammatory reaction of the skin, and soothe symptoms such as redness, pain, and itching caused by inflammation, promoting the repair and regeneration of the skin; balance the sebum secretion of the skin, relieve skin sensitivity and discomfort, promote blood circulation of the skin, and eliminate fatigue; promote the synthesis of collagen and the generation of elastic fibers in the skin, reduce the formation of fine lines and wrinkles, increase the elasticity and luster of the skin; scavenge free radicals, protect the skin from oxidative damage, delay the aging process of the skin, reduce the formation of skin spots and dullness, and make the skin brighter and more uniform. In addition, the preparation method of the massage oil of the present invention has mild reaction conditions, simple operation steps, and is easy to scale up for industrial production. Detailed implementation manners

[0031] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0032] The following is elaborated in conjunction with specific embodiments to illustrate the actual effects of the present invention solution.

[0033] Unless otherwise specified, the test methods used in the examples are all conventional methods; the materials, reagents, equipment, etc. used, unless otherwise specified, can all be obtained from commercial channels.

[0034] The raw materials used in the following examples and comparative examples are described as follows, but are not limited to these materials:

[0035] The said deep-sea two-joint shepherd's purse seed oil, rusty rose seed oil, evening primrose oil, sunflower seed oil, olive fruit oil, European hazelnut oil and macadamia nut oil are all purchased from Northstar Lipids(UK)Ltd (North Star, UK);

[0036] The said extract of the root bark of Acanthopanax gracilistylus, extract of Jasminum grandiflorum, lemon peel oil, Artemisia argyi leaf oil, Michelia alba leaf oil, bergamot fruit oil, ginger root oil, Angelica archangelica root oil, Ligusticum chuanxiong oil, Pelargonium graveolens oil, extract of Liquidambar formosana, extract of Spatholobus suberectus, extract of Phryma leptostachya, extract of the root of Panax notoginseng, borage seed oil, sea buckthorn fruit oil, lithospermum oil, extract of the root of Acanthopanax senticosus, extract of Jasminum sambac are all purchased from Guangzhou Xinsi Mei Biotechnology Co., Ltd;

[0037] The said tocopheryl acetate is purchased from BASF(China)Co.Ltd.Shanghai (BASF, Germany);

[0038] The said extract of the root of Glycyrrhiza glabra, stearyl glycyrrhetinate, vanillyl butyl ether are purchased from Xinjiang Caozimuye Pharmaceutical Co., Ltd.

[0039] In the following examples and comparative examples, the preparation method of the vegetable oil essential oil composition involved is as follows: Mix the deep-sea Crambe oil, Rosa rubiginosa seed oil, evening primrose oil, Acanthopanax gracilistylus root bark extract, and Jasminum grandiflorum extract evenly, and that's it.

[0040] The raw materials (by weight parts) of the vegetable oil essential oil composition in the following examples and comparative examples are shown in Tables 1 and 2.

[0041] Table 1 Raw material ratio (weight parts) of the vegetable oil essential oil composition in the examples of the present invention

[0042] Component Crambe abyssinica seed oil Rosa rubiginosa seed oil Evening primrose oil Acanthopanax gracilistylus root bark extract Jasminum grandiflorum extract Example 1 56 17 15 8 4 Example 2 60.96 15.5 13.57 6.85 3.12 Example 3 67.14 13.16 11.28 5.64 2.78 Example 4 78.8 9 8 3 1.2 Example 5 69.5 10 10 7.5 3 Example 6 80 5 11 2 2 Example 7 50 20 10 15 2

[0043] Table 2 Raw material ratio (weight parts) of the vegetable oil essential oil composition in the comparative examples of the present invention

[0044]

[0045]

[0046] In the following application examples and application comparative examples, the preparation method of the massage oil involved is as follows:

[0047] (1) Mix sunflower seed oil, olive fruit oil, hazelnut oil, macadamia nut oil, vegetable oil essential oil composition, and squalane evenly to obtain mixture A;

[0048] (2) Mix lemon peel oil, mugwort leaf oil, Michelia alba leaf oil, bergamot fruit oil, ginger root oil, Angelica archangelica root oil, Ligusticum wallichii oil, Pelargonium graveolens oil, and extracts of Clerodendrum cyrtophyllum Turcz., Spatholobus suberectus Dunn, Herba Speranskiae tuberculatae, and Panax notoginseng root evenly to obtain mixture B;

[0049] (3) Mix tocopheryl acetate, stearoyl glycyrrhetinate, Glycyrrhiza glabra root extract, and vanillyl butyl ether evenly to obtain mixture C;

[0050] (4) Mix mixture A, mixture B, and mixture C, stir at a speed of 200 rpm for 3 h, and then let it stand for 12 h to obtain the massage oil.

[0051] The raw materials (by weight percentage) of the massage oil in the following application examples and application comparative examples are shown in Table 3 and so on.

[0052] Table 3: Raw material ratio (by weight percentage) of the massage oil in the application examples and application comparative examples of the present invention

[0053]

[0054]

[0055] Test Example 1: Anti-inflammatory effect test

[0056] (1) Materials and methods:

[0057] (I) Test samples: First, the essential oil compositions of vegetable oils in Examples 1-7 and Comparative Examples 1-12 were prepared into a solution with a mass concentration of 30% using dimethyl sulfoxide, and then into a solution with a mass concentration of 1% using DMEM-H complete medium;

[0058] (II) Test principle: Rheumatoid arthritis is an autoimmune disease characterized by chronic inflammation of the synovium. Untreated rheumatoid arthritis leads to persistent joint destruction, resulting in disability, low quality of life, and increased mortality. Fibroblast-like synoviocytes (FLS) and inflammatory cells, such as macrophages and T cells, produce pro-inflammatory cytokines, such as IL-1β and TNF-α, which play a key role in the pathogenesis of rheumatoid arthritis. In this test example of the present invention, a human rheumatoid arthritis fibroblast-like synoviocyte (RA-FLS) model was used to evaluate the inhibitory effect of the essential oil compositions of vegetable oils of the present invention on IL-1β.

[0059] (III) Test procedure: RA-FLS cells in the logarithmic growth phase (purchased from: Cell Resource Center, Institute of Basic Medicine, Chinese Academy of Medical Sciences) were added to a 6-well culture plate, with 2×10 5 cells added to each well, and DMEM-H complete medium was added. The cells were cultured for 24 h under the conditions of 37 °C, 5% CO2, and saturated humidity; the medium was aspirated, 1 mL of DMEM-H complete medium was added to the blank control group and the model group, and 1 mL of the corresponding test sample was added to the sample group. The cells were cultured for 24 h under the conditions of 37 °C, 5% CO2, and saturated humidity. Subsequently, the model group and the sample group were stimulated with 1 mL of 10 ng / mL lipopolysaccharide (LPS) for 6 hours, and the supernatant and cell lysate were collected. The IL-1β in the cell culture supernatant was quantified by an IL-1β enzyme-linked immunosorbent assay (ELISA) kit, and the content of IL-1β was measured at 450 nm by an enzyme-labeled instrument. Taking the IL-1β content of the cells in the blank control group as 1.00, the relative content of IL-1β in other groups was calculated.

[0060] (2) Test results: As shown in Table 4, the cells in the model group were not treated with any test samples. After being stimulated with LPS, the IL-1β value increased significantly, which was 1.83 times that of the blank control group. After the RA-FLS cells treated with the test samples were stimulated with LPS, the IL-1β value decreased to varying degrees, indicating that the test samples could inhibit the expression of IL-1β in RA-FLS cells, suggesting that the test samples had excellent anti-inflammatory effects. The expression level of the essential oil compositions of vegetable oils in the examples was equivalent to or even lower than that of the blank control group.

[0061] Test Example 2: Soothing efficacy test

[0062] (1) Materials and methods:

[0063] (I) Test samples: First, the essential oil compositions of vegetable oils in Examples 1-7 and Comparative Examples 1-12 were prepared into a solution with a mass concentration of 30% using dimethyl sulfoxide, and then into a solution with a mass concentration of 1% using deionized water.

[0064] (II) Test principle: Hyaluronidase is a participant in type I allergic reactions and is strongly correlated with inflammation and allergy. The soothing effect of the test samples was reflected by testing their inhibitory effect on hyaluronidase activity in this test example.

[0065] (III) Test procedure: The hyaluronidase inhibition rate of each test sample was calculated through the Elson-Morgan hyaluronidase inhibition experiment.

[0066] Four groups, namely A, B, C, and D, were set up, with 3 parallel groups in each group.

[0067] Group A was the reaction solution containing the sample and the enzyme: 0.1 mL of 2.5 mmol / L calcium chloride solution, 0.5 mL of 600 U / mL hyaluronidase, 0.1 mL of acetate buffer solution, 0.5 mL of the sample solution, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetic acid acetone solution, and 1 mL of Ehrlich reagent were successively added to the test tube.

[0068] Group B was the reaction solution containing the sample but without the enzyme: 0.1 mL of 2.5 mmol / L calcium chloride solution, 0.5 mL of deionized water, 0.1 mL of acetate buffer solution, 0.5 mL of the sample solution, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetic acid acetone solution, and 1 mL of Ehrlich reagent were successively added to the test tube.

[0069] Group C was the reaction solution containing the enzyme but without the sample: 0.1 mL of 2.5 mmol / L calcium chloride solution, 0.5 mL of 600 U / mL hyaluronidase, 0.1 mL of acetate buffer solution, 0.5 mL of deionized water, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetic acid acetone solution, and 1 mL of Ehrlich reagent were successively added to the test tube.

[0070] Group D was the reaction solution without the sample and the enzyme: 0.1 mL of 2.5 mmol / L calcium chloride solution, 1 mL of deionized water, 0.1 mL of acetate buffer solution, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetic acid acetone solution, and 1 mL of Ehrlich reagent were successively added to the test tube.

[0071] Mix the above four groups of reaction solutions and place them in boiling water, ice water, and room temperature environments successively for reaction. Then add P-DAB color reagent to the four tubes. After the solution reaction is complete, measure the absorbance value of each group at a wavelength of 530 nm. Calculate the inhibition rate of hyaluronidase through the absorbance of each group.

[0072] The calculation formula for the inhibition rate of hyaluronidase is as follows:

[0073]

[0074] Among them, A is the absorbance of the reaction solution containing the sample and the enzyme; B is the absorbance of the reaction solution containing the sample and without the enzyme; C is the absorbance of the reaction solution containing the enzyme and without the sample; D is the absorbance of the reaction solution without the sample and the enzyme.

[0075] (2) Test results: As shown in Table 4, the test results show that the vegetable oil essential oil composition of the embodiment of the present invention can well inhibit the activity of hyaluronidase, indicating that the vegetable oil essential oil composition of the embodiment of the present invention has a good soothing effect.

[0076] Test Example 3: Anti-wrinkle performance test

[0077] (1) Materials and methods:

[0078] (I) Test samples: First, use dimethyl sulfoxide to prepare a solution with a mass concentration of 30% of the vegetable oil essential oil compositions of Examples 1-7 and Comparative Examples 1-12, and then use deionized water to prepare a solution with a mass concentration of 1%.

[0079] (II) Test principle: Elastase is the most important enzyme in the matrix metalloproteinase family, mainly synthesized and secreted by fibroblasts, and can degrade elastin in the skin, leading to skin aging. In this test example, the anti-wrinkle and firming effects are characterized by evaluating the inhibition rate of the test samples on elastase.

[0080] (III) Test steps: Prepare a Tricine buffer solution (50 mmol / L, pH 7.5) containing 400 mmol / L NaCl and 10 mmol / L CaCl2, and use the Tricine buffer solution to prepare an elastase solution with a concentration of 0.8 U / mL and an N-[3-(2-furyl)acryloyl]-leucine-glycine-proline-alanine (FALGPA) solution with a concentration of 2 mmol / L.

[0081] Group A is the test group: Mix 40 μL of the sample solution with 100 μL of Tricine buffer, then add 20 μL of elastase solution at 0.8 U / mL. After mixing, incubate at a constant temperature of 25 °C for 15 min. Then add 40 μL of 2 mmol / L FALGPA solution. After 15 min, measure the absorbance value at 335 nm, and perform parallel measurements 3 times.

[0082] Group B is the reaction solution without enzyme but containing the sample: Mix 40 μL of the sample solution with 100 μL of Tricine buffer, then add 20 μL of elastase solution at 0.8 U / mL. After mixing, incubate at a constant temperature of 25 °C for 15 min. Then add 40 μL of 2 mmol / L FALGPA solution. After 15 min, measure the absorbance value at 335 nm, and perform parallel measurements 3 times.

[0083] Group C is the reaction solution without sample but containing enzyme: Mix 40 μL of purified water with 100 μL of Tricine buffer, then add 20 μL of elastase solution at 0.8 U / mL. After mixing, incubate at a constant temperature of 25 °C for 15 min. Then add 40 μL of 2 mmol / L FALGPA solution. After 15 min, measure the absorbance value at 335 nm, and perform parallel measurements 3 times.

[0084] Group D is the reaction solution without sample and enzyme: Mix 40 μL of purified water with 100 μL of Tricine buffer, then add 20 μL of purified water. After mixing, incubate at a constant temperature of 25 °C for 15 min. Then add 40 μL of 2 mmol / L FALGPA solution. After 15 min, measure the absorbance value at 335 nm, and perform parallel measurements 3 times.

[0085] Calculate the inhibition rate of the sample on elastase according to the following formula:

[0086]

[0087] Where A is the absorbance of the test group; B is the absorbance of the reaction solution without enzyme but containing the sample; C is the absorbance of the reaction solution without sample but containing enzyme; D is the absorbance of the reaction solution without sample and enzyme.

[0088] (2) Test results: As shown in Table 4, the essential oil composition of vegetable oil in the examples of the present invention can well inhibit the activity of elastase, indicating that the essential oil composition of vegetable oil in the examples of the present invention has good anti-wrinkle effects.

[0089] Test Example 4: Antioxidant performance test

[0090] (1) Materials and methods:

[0091] (I) Test samples: First, use dimethyl sulfoxide to prepare a solution with a mass concentration of 30% of the vegetable oil essential oil compositions of Examples 1-7 and Comparative Examples 1-12, and then use deionized water to prepare a solution with a mass concentration of 1%.

[0092] (II) Test steps: Take 5 mL of Tris-HCl buffer solution with a pH of 8.2 in a stoppered test tube, preheat it in a water bath at 25 °C for 20 min, and then sequentially add 2 mL of the sample solution and 0.5 mL of 5×10 -3 mol / L pyrogallol solution (prepared with 1×10 -2 mol / L HCl solution). Immediately shake well and react in a water bath at 25 °C for 4 min. Immediately measure its absorbance A1 at 320 nm; use 0.5 mL of 1×10 -2 mol / L HCl solution to replace the pyrogallol solution to measure the absorbance A2; use 2 mL of pure water to replace the sample solution to measure the absorbance A3. Calculate the scavenging rate of ·O2 - according to the following formula.

[0093]

[0094] Among them, A1 is the absorbance of the sample for scavenging free radicals; A3 is the absorbance of the solution without the sample; A2 is the absorbance of the sample at the measurement wavelength.

[0095] (2) Test results: As shown in Table 4, the vegetable oil essential oil compositions of the examples of the present invention can eliminate ·O2 - free radicals and show excellent antioxidant effects.

[0096] Table 4: Relative content of IL-1β in test group cells of examples and comparative examples

[0097]

[0098]

[0099] It can be seen from the results in Table 4 that the effects of the vegetable oil essential oil compositions of Examples 1-7 of the present invention are significantly better than those of Comparative Examples 1-12. That is, in the vegetable oil essential oil compositions of the examples of the present invention, the synergistic effect among Crambe abyssinica seed oil, Rosa rubiginosa seed oil, evening primrose oil, Acanthopanax gracilistylus root bark extract, and Jasminum grandiflorum extract can well inhibit the expression of IL-1β in RA-FLS cells, inhibit the activity of hyaluronidase, inhibit the activity of elastase, and eliminate ·O 2- free radicals. It has excellent anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects. If one of the components is missing, or the components are replaced, the anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects of the composition will become worse.

[0100] Test Example 5: Test on the usability and efficacy of massage oil

[0101] (1) Test samples: Massage oils of Application Examples 1-4 and Application Comparative Examples 1 and 2;

[0102] (2) Test steps: Select 60 volunteers aged 40-60 with arthritis problems (10 volunteers use one test sample each). The volunteers take an appropriate amount of massage oil and apply it to the arthritic parts, rub for 2 minutes, and use the product continuously for 2 weeks, once a day. The volunteers evaluate the usability and efficacy of the sample after using it. The scoring standard is: 0 means no effect, 10 means very good effect, and the score can take any value from 0 to 10. The test results are averaged;

[0103] (3) The test results are shown in Table 6. The test results show that after two weeks of using the massage oil of the application examples of the present invention, the painful parts of the volunteers' arthritis have been well relieved, the fatigue has been well eliminated, and after two weeks of use, the skin of the corresponding parts has become better, indicating that the massage oil provided by the present invention can not only play the role of strengthening the zongjin, promoting blood circulation and removing blood stasis, but also play the effect of skin care.

[0104] Table 6: Scoring table for the usability and efficacy of massage oils of application examples and application comparative examples

[0105]

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A vegetable oil essential oil composition, characterized in that The invention comprises the following components in parts by weight: 50-80 parts of shepherd's purse seed oil, 5-20 parts of rust red rose seed oil, 8-15 parts of evening primrose oil, 2-15 parts of Acanthopanax scapularis root bark extract and 1.2-5 parts of Jasmine flower extract.

2. The vegetable oil essential oil composition according to claim 1, wherein The invention comprises the following components in parts by weight: 56-78.8 parts of shepherd's purse seed oil, 9-17 parts of rust red rose seed oil, 8-15 parts of evening primrose oil, 3-8 parts of Acanthopanax scapularis root bark extract and 1.2-4 parts of Jasmine flower extract.

3. The vegetable oil essential oil composition according to claim 1, wherein The mass ratio of the evening primrose oil to the Acanthopanax gracilis root bark extract is 1:(0.15-1.5).

4. The method for preparing the vegetable oil essential oil composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: The deep sea camellia seed oil, rust red rose seed oil, evening primrose oil, Acanthopanax styli root bark extract and Jasmine flower extract are mixed evenly to prepare the product.

5. A massage oil, characterized in that: The massage oil comprises the plant oil essential oil composition according to any one of claims 1 to 3.

6. The massage oil according to claim 5, characterized in that The invention comprises the following components in weight percentage: 9.5%-18% of the plant oil essential oil composition, 60%-75% of the base oil, 5%-10% of the blending oil, 1%-2.5% of the conditioner, 0.2%-1% of the antioxidant, 0.05%-0.3% of the soothing agent, 0.03%-0.1% of the thermal agent and the balance of the solvent.

7. The massage oil according to claim 6, characterized in that The base oil includes at least one of sunflower seed oil, olive oil, filbert oil, and macadamia seed oil; the blending oil includes at least one of lemon peel oil, wormwood oil, brandy leaf oil, bergamot fruit oil, ginger root oil, angelica root oil, chuanxiong oil, and geranium oil; the conditioning agent includes at least one of Rhizoma Lulutong extract, Millettia spatholobi extract, Herba Polygoni Multiflori extract, and Panax notoginseng root extract; the soothing agent includes Glycyrrhiza glabra root extract and / or stearyl glycyrrhizinate.

8. The method for preparing the massage oil according to any one of claims 5 to 7, characterized in that: The following steps are involved: (1) uniformly mixing the plant oil essential oil composition, base oil, and solvent to obtain a mixture A; (2) uniformly mixing the blended oil and the conditioning agent to obtain a mixture B; (3) mixing the antioxidant, soothing agent, and thermal agent uniformly to obtain a mixture C; (4) Mix the mixture A, mixture B and mixture C, stir, and let stand to obtain a mixture.

9. The method for preparing massage oil according to claim 8, characterized in that: The stirring time is 2h-5h; the standing time is 10h-15h.

10. Use of the plant essential oil composition according to any one of claims 1 to 3 and the massage oil according to any one of claims 5 to 7 in skin care products and health care products.

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