Lunninghamia lanceolata tea fermentation composition for repairing staying up late and application thereof

Abstract

The invention relates to a snow lotus tea fermentation composition for repairing staying up late and application, and belongs to the technical field of cosmetics. The Xiaojia tea fermentation composition disclosed by the invention is prepared from the following components in parts by weight: 3 to 15 parts of black tea fermentation filtrate, 0.01 to 0.6 part of theanine and 0.5 to 3 parts of Xiaojia bulb extract. According to the composition comprehensively utilizing the black tea fermentation product filtrate, the theanine and the Xueshua lotus bulb extract, when the three components are combined, the following efficacy breakthroughs can be realized: firstly, the antioxidant efficacy is enhanced, and the damage of free radicals to the skin when staying up late can be more effectively resisted; and secondly, repairing the skin barrier, maintaining the normal physiological function of the skin and relieving the skin damage caused by staying up late. Thirdly, efficient whitening is achieved, darkness and darkness caused by staying up late are relieved, and the skin looks more bright.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to a Leucojum aestivum tea fermentation composition for staying-up-late repair and its application. Background Art

[0002] The components in the tea fermentation filtrate can stimulate the activity of the body's own antioxidant enzyme system, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), etc. These enzymes can help scavenge free radicals in the body and maintain the normal metabolism and function of cells. By enhancing the activity of antioxidant enzymes. Although the tea fermentation filtrate and theanine have strong antioxidant capabilities, there is a lack of research on scavenging free radicals during staying up late and preventing the attack of a large number of free radicals generated during staying up late on cells, and it is impossible to confirm whether their antioxidant effects can target and improve the skin problems generated during staying up late.

[0003] Theanine can cross the blood-brain barrier and enter the brain, affect the synthesis and release of γ-aminobutyric acid (an inhibitory neurotransmitter), and can reduce the activity of neurons and reduce the over-excitation of the brain, achieving the effect of helping sleep. Moreover, theanine can protect cells from oxidative stress through various pathways. It can regulate the signal transduction pathway in cells, activate the expression of antioxidant genes, and enhance the antioxidant capacity of cells.

[0004] Leucojum aestivum is a mild plant ingredient. Current research shows that Leucojum aestivum can reduce oxidative damage and bring certain anti-aging activity by increasing the expression of antioxidant enzymes in keratinocytes. Moreover, research shows that after using Leucojum aestivum for 3.5 days and 7 days, the inhibition rates of melanin synthesis reach 45% and 70% respectively, proving that Leucojum aestivum has a certain melanin-inhibiting effect. Although Leucojum aestivum has been proven to be able to inhibit the production of melanocytes, there is also a lack of research on whether it can improve the skin dullness problem after staying up late. And only from the existing theoretical research, it is impossible to judge whether it can play an improving role in the skin before and after staying up late when combined with the other two ingredients. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Leucojum aestivum tea fermentation composition for staying-up-late repair with the advantages of synergistic antioxidant and skin barrier repair, and its application.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] In the first aspect, the present invention provides a Leucojum aestivum tea fermentation composition, comprising the following components in parts by weight: 3-15 parts of black tea fermentation filtrate, 0.01-0.6 part of theanine, and 0.5-3 parts of Leucojum aestivum bulb extract.

[0008] By selecting the fermentation filtrate of black tea, theanine, and the bulb extract of Leucojum aestivum L. with specific components, the obtained Leucojum aestivum L. tea fermentation composition has the advantage of synergistic antioxidant and additionally has the effects of repairing the skin barrier and whitening the skin.

[0009] As a preferred embodiment of the Leucojum aestivum L. tea fermentation composition of the present invention, it includes the following components in parts by weight: 10 parts of black tea fermentation filtrate, 0.1 part of theanine, and 1 part of the bulb extract of Leucojum aestivum L.

[0010] The black tea fermentation filtrate is the fermentation product filtrate of fermenting black tea with yeast and / or acetic acid bacteria, and the INCI name is yeast / acetic acid bacteria / black tea fermentation product.

[0011] In a second aspect, the present invention provides the application of the Leucojum aestivum L. tea fermentation composition in the preparation of antioxidant products.

[0012] In a third aspect, the present invention provides the application of the Leucojum aestivum L. tea fermentation composition in the preparation of skin barrier repair products.

[0013] In a fourth aspect, the present invention provides the application of the Leucojum aestivum L. tea fermentation composition in the preparation of skin whitening products.

[0014] Furthermore, the product includes cosmetics.

[0015] Furthermore, the cosmetics include at least one of cleansing products, lotions, creams, and masks.

[0016] In a fifth aspect, the present invention provides a cosmetic, which contains the Leucojum aestivum L. tea fermentation composition.

[0017] Furthermore, the cosmetic also contains cosmetic adjuvants.

[0018] Furthermore, the cosmetic includes the following components in parts by weight: 3.51 - 18.6 parts of the Leucojum aestivum L. tea fermentation composition and 81.4 - 96.49 parts of cosmetic adjuvants.

[0019] As a preferred embodiment of the cosmetic of the present invention, the cosmetic includes the following components in parts by weight: 11.1 parts of the Leucojum aestivum L. tea fermentation composition and 88.9 parts of cosmetic adjuvants.

[0020] Furthermore, the cosmetic adjuvants include at least one of emollients, dispersants, humectants, preservatives, emulsification stabilizers, thickeners, soothers, chelating agents, pH regulators, and solvents.

[0021] Furthermore, the thickener includes acrylic acid (ester) / C10-30 alkanol acrylate cross-linked polymer and / or sodium acrylate / acryloyldimethyl taurate copolymer; the chelating agent includes disodium EDTA; the humectant includes at least one of glycerol, sodium hyaluronate, butanediol and 1,3-propanediol; the soothing agent includes allantoin; the preservative includes p-hydroxyacetophenone and / or phenoxyethanol; the emollient includes at least one of triglyceride caprylate / caprate, polydimethylsiloxane and dicaprylyl carbonate; the pH regulator includes aminomethylpropanol; the emulsion stabilizer includes C14-22 alcohol C12-20 alkyl glucoside; the solvent includes water.

[0022] As a preferred embodiment of the cosmetic of the present invention, the cosmetic adjuvant includes the following components in parts by weight: 0.15 part of acrylic acid (ester) / C10-30 alkanol acrylate cross-linked polymer, 0.02 part of disodium EDTA, 0.1 part of allantoin, 6 parts of glycerol, 0.05 part of sodium hyaluronate, 1 part of polydimethylsiloxane, 2 parts of dicaprylyl carbonate, 3 parts of triglyceride caprylate / caprate, 1.8 parts of C14-22 alcohol C12-20 alkyl glucoside, 0.3 part of sodium acrylate / acryloyldimethyl taurate copolymer, 0.04 part of aminomethylpropanol, 3 parts of butanediol, 0.4 part of p-hydroxyacetophenone, 0.3 part of 1,3-propanediol, 0.2 part of phenoxyethanol and 70.54 parts of water, to prepare an emulsion.

[0023] In a sixth aspect, the present invention provides a method for preparing the cosmetic, comprising the following steps:

[0024] S1: Stir the raw materials of phase B evenly at 500-800 rpm and adjust the temperature to 80-85 °C;

[0025] S2: Mix the raw materials of phase A with the phase B raw materials treated in step S1, homogenize at 2200 rpm for 2-3 min; keep stirring at 800-1200 rpm, adjust the temperature to 80-85 °C, keep warm and stir for 15 min, and stir until dissolved evenly;

[0026] S3: Mix the raw materials of phase C with the product treated in step S2, homogenize at 2500 rpm for 2-4 min, and stir evenly at 800 rpm;

[0027] S4: Adjust the temperature of the product treated in step S3 to 55 °C, add the raw materials of phase D, stir at 800-1000 rpm for 3-5 min, and stir until dissolved evenly; adjust the temperature to 45 °C, add the raw materials of phase E, and stir evenly at 600-1000 rpm; adjust the temperature to 38 °C, filter, and obtain the cosmetic.

[0028] The raw material of phase A includes at least one of acrylic (ester) / C10-30 alkanol acrylate cross-linked polymer, disodium EDTA, allantoin, glycerin, sodium hyaluronate, polydimethylsiloxane and water;

[0029] The raw material of phase B includes at least one of dicaprylyl carbonate, caprylic / capric triglyceride and C14-22 alcohol C12-20 alkyl glucoside;

[0030] The raw material of phase C includes at least one of sodium acrylate / sodium acryloyldimethyl taurate copolymer, aminomethyl propanol and water;

[0031] The raw material of phase D includes butanediol and / or p-hydroxyacetophenone;

[0032] The raw material of phase E includes the Leucojum aestivum tea fermentation composition described in claim 1 or 2, and also includes at least one of 1,3-propanediol, phenoxyethanol and water.

[0033] Furthermore, each component in the raw material of phase A, phase B, phase C, phase D or phase E can be first mixed and stirred evenly, and then the cosmetic is prepared.

[0034] Furthermore, in step S4, it is filtered through 190-210 meshes, preferably 200 meshes.

[0035] Furthermore, step S4 also includes inspecting, filling and packaging the filtered product to obtain the final commercially available emulsion product.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0037] The present invention conducts research on staying-up-late repair, aiming to provide a composition that comprehensively utilizes the filtrate of black tea fermentation product, theanine and the extract of Leucojum aestivum bulb to solve the deficiencies of each component in the existing research technology. The filtrate of tea fermentation product has a powerful antioxidant effect and can effectively scavenge free radicals generated after staying up late; theanine can enhance the antioxidant ability of cells and prevent the attack of a large number of free radicals generated during staying up late on cells; Leucojum aestivum has a certain melanin inhibition effect and can effectively solve the problem of dull skin after staying up late. When the three are combined, the following efficacy breakthroughs can be achieved. First, the antioxidant effect is enhanced, and it can more effectively fight against the damage of free radicals to the skin during staying up late. Second, it breaks through to repair the skin barrier, maintains the normal physiological functions of the skin, and relieves the damaged skin caused by staying up late. Third, it realizes high-efficiency whitening, reduces the dullness caused by staying up late, and makes the skin look more radiant. This combination brings new possibilities for the application of cosmetics and is expected to bring a better skin care experience to consumers. Description of the Drawings

[0038] Figure 1An emulsion prepared from the Leucojum aestivum tea fermentation composition of the present invention. Detailed implementation manners

[0039] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Other materials, reagents, etc. used in the embodiments can be obtained from commercial channels without special instructions.

[0040] The black tea fermentation filtrate of the present invention, with the INCI name of Saccharomyces / Xylacetic acid bacteria / Black tea fermentation product, is purchased from Guangzhou Xiyuan Biotechnology Co., Ltd.

[0041] The theanine of the present invention is purchased from Bozhou Mingqi Biotechnology Co., Ltd.

[0042] The Leucojum aestivum bulb extract of the present invention, namely Leucojum aestivum bulb extract, is purchased from Lucas Meyer Cosmetics.

[0043] Examples 1-11, Comparative examples 1-11

[0044] The composition components of the Leucojum aestivum tea fermentation composition in Examples 1-11 and Comparative examples 1-11 are shown in Tables 1 and 2.

[0045] Table 1 Composition components of the Leucojum aestivum tea fermentation composition in Examples 1-11 (parts by weight)

[0046]

[0047] Table 2 Composition components of the Leucojum aestivum tea fermentation composition in Comparative examples 1-11 (parts by weight)

[0048]

[0049] “ / ” in the table means not added.

[0050] Application Examples 1-11, Application Comparative Examples 1-11

[0051] Prepare emulsions according to the Leucojum aestivum tea fermentation compositions in Examples 1-11 and Comparative examples 1-11.

[0052] I. Emulsion formula:

[0053] The emulsion consists of 0.15% acrylate / C10-30 alkanol acrylate cross-linked polymer, 0.02% disodium EDTA, 0.1% allantoin, 6% glycerol, 0.05% sodium hyaluronate, 1% polydimethylsiloxane, 2% dicaprylyl carbonate, 3% caprylic / capric triglyceride, 1.8% C14-22 alcohol C12-20 alkyl glucoside, 0.3% acrylate / sodium acryloyldimethyltaurate copolymer, 0.04% aminomethylpropanol, 3% butanediol, 0.4% p-hydroxyacetophenone, 0.3% 1,3-propanediol, 0.2% phenoxyethanol, water and Leucojum aestivum tea fermentation composition. The water is added to make up the balance, and the above are in weight percentages.

[0054] Among them, in the emulsions of Application Examples 1 to 11, the weight percentages of the black tea fermentation product filtrate, theanine and Leucojum aestivum bulb extract in the Leucojum aestivum tea fermentation composition are shown in Table 3, and the obtained emulsions are as Figure 1 shown; in the emulsions of Comparative Examples 1 to 11, the weight percentages of the black tea fermentation product filtrate, theanine, Leucojum aestivum bulb extract and astaxanthin in the Leucojum aestivum tea fermentation composition are shown in Table 4. Astaxanthin is also a component with strong antioxidant power, so in Comparative Example 11, it is used to replace the Leucojum aestivum bulb extract for comparison.

[0055] Table 3 Composition components (weight percentages) of the Leucojum aestivum tea fermentation composition in Application Examples 1 to 11

[0056]

[0057] Table 4 Composition components (weight percentages) of the Leucojum aestivum tea fermentation composition in Comparative Examples 1 to 11

[0058]

[0059] II. Emulsion preparation method:

[0060] 1. Pretreatment

[0061] (1) Disperse acrylate / C10-30 alkanol acrylate cross-linked polymer, disodium EDTA, allantoin, glycerol, sodium hyaluronate, polydimethylsiloxane and water evenly and set aside to obtain the pretreated Phase A raw materials.

[0062] (2) Disperse acrylate / sodium acryloyldimethyltaurate copolymer, aminomethylpropanol and water evenly and set aside to obtain the pretreated Phase C raw materials.

[0063] (3) Disperse butanediol and p-hydroxyacetophenone evenly and set aside to obtain the pretreated Phase D raw materials.

[0064] (4) Pre-disperse 1,3-propanediol, phenoxyethanol, water and Leucojum aestivum tea fermentation composition evenly and set aside to obtain the pretreated Phase E raw materials.

[0065] 2. Preparation

[0066] (1) Add dioctyl carbonate, caprylic / capric triglyceride, and C14 - 22 alcohol C12 - 20 alkyl glucoside into the emulsifying pan in sequence, stir at 500 - 800 rpm, and heat up to 80 - 85 °C to obtain the raw material of Phase B.

[0067] (2) Pour the pretreated raw material of Phase A into the raw material of Phase B in step (1), homogenize at 2200 rpm for 2 - 3 min; keep stirring at 800 - 1200 rpm, heat up to 80 - 85 °C, and keep stirring for 15 min to stir and dissolve evenly.

[0068] (3) Pour the pretreated raw material of Phase C into the product obtained in step (2), homogenize at 2500 rpm for 2 - 4 min, and stir evenly at 800 rpm.

[0069] (4) Cool the product obtained in step (3) to 55 °C, add the pretreated raw material of Phase D, stir at 800 - 1000 rpm for 3 - 5 min to stir and dissolve evenly; after cooling to 45 °C, add the pretreated raw material of Phase E, and stir evenly at 600 - 1000 rpm.

[0070] (5) Take the product obtained in step (4) for in - process inspection; after passing the inspection, cool the temperature to 38 °C and filter through a 200 - mesh sieve for discharging.

[0071] (6) Let the discharged product in step (5) stand still for semi - finished product inspection.

[0072] (7) After the semi - finished product in step (6) passes the inspection, carry out filling and packaging to obtain the finished product.

[0073] (8) Inspect the finished product in step (7).

[0074] Test Example 1 Antioxidant Test (DPPH Method)

[0075] 1. Test Samples: Emulsion samples prepared in Application Examples 1 - 11 and Application Comparative Examples 1 - 11.

[0076] 2. Test Principle: Since DPPH free radicals have a single electron, have a strong absorption at 517 nm, and its alcohol solution is purple. When there is a free radical scavenger, due to the pairing of its single electron, its absorption gradually disappears, and the degree of fading is in a quantitative relationship with the number of electrons it accepts. Therefore, rapid quantitative analysis can be carried out with a spectrophotometer.

[0077] 3. Reagents and Materials

[0078] (1) Reagents: 0.2 mM DPPH solution (1,1 - diphenyl - 2 - trinitrophenylhydrazine, C18 H 12 N5O6): Weigh 0.007875 g of DPPH powder and dissolve it in 100 mL of absolute ethanol to prepare a 0.2 mM DPPH solution; VC solution (positive control group): Dilute VC with deionized water to 0.001 mg / mL, 0.002 mg / mL, 0.004 mg / mL, 0.006 mg / mL, 0.008 mg / mL, 0.01 mg / mL, 0.02 mg / mL, and 0.5 mg / mL in turn, and place them in brown bottles for later use.

[0079] (2) Instruments and equipment: Analytical balance (accurate to 0.001 g), ultrasonic cleaner, ultraviolet-visible spectrophotometer.

[0080] 4. Test procedure

[0081] Divide into four sample tubes and add samples in sequence according to Table 5.

[0082] Positive control group: Set up a vitamin C standard control tube, and add 4.5 mL of DPPH solution and 0.5 mL of vitamin C solution to each tube.

[0083] After mixing the solutions in each tube, react them at room temperature in the dark for 30 min. Zero the spectrophotometer with distilled water at a wavelength of 517 nm, measure the absorbance, and record the data (note that in data processing, the absolute difference between two independent measurement results obtained under repetitive conditions shall not exceed 10% of the arithmetic mean).

[0084] Calculate the scavenging rate (P) according to formula (1):

[0085]

[0086] In formula (1),

[0087] T: Absorbance value of the sample tube, that is, the absorbance value of the solution after the test sample reacts with DPPH;

[0088] T0: Background absorbance value of the test sample;

[0089] C: Absorbance value of the DPPH tube, that is, the absorbance value of the DPPH solution without adding the test sample;

[0090] C0: Background absorbance value of the solvent (absolute ethanol).

[0091] Table 5

[0092] Reagent T - Sample tube <![CDATA[T0 - Sample blank tube]]> C — Control tube <![CDATA[C0—Control blank]]> Test sample (mL) 2 2 / / Absolute ethanol (mL) / 2 2 4 DPPH solution (mL) 2 / 2 /

[0093] Table 6

[0094]

[0095]

[0096] The results are shown in Table 6. After verification by the antioxidant experiment, the examples of the present invention all have good effects of scavenging free radicals and antioxidation. Among them, the emulsion prepared with the Leucojum aestivum tea fermentation composition of Example 9 of the present invention has the best antioxidant effect. The increase in the content of the black tea fermentation filtrate helps its synergistic effect with other components and improves the antioxidant efficacy of the Leucojum aestivum tea fermentation composition. If astaxanthin is used to replace the Leucojum aestivum bulb extract, its DPPH scavenging rate is only 8.52±0.73%, which is much lower than that of Example 9 and even lower than that of Application Comparative Example 3 without astaxanthin or Leucojum aestivum bulb extract.

[0097] Test Example 2 Antioxidant Test (SOD Method)

[0098] 1. Test samples: Emulsions prepared from Application Examples 1 to 11 and Application Comparative Examples 1 to 11.

[0099] 2. Principle: SOD is a metal enzyme widely present in organisms and is an important oxygen free radical scavenger. It can catalyze the dismutation of superoxide anions to generate H2O2 and O2. SOD is not only a superoxide anion scavenging enzyme but also the main enzyme for generating H2O2 and plays an important role in the biological antioxidant system. Superoxide anions (O2 - ) are generated through the xanthine and xanthine oxidase reaction system. O2 - can reduce nitroblue tetrazolium to generate blue formazan, which has an absorption at 560 nm; SOD can scavenge O2 - , thus inhibiting the formation of formazan; the darker the blue color of the reaction solution, the lower the SOD activity, and vice versa.

[0100] 3. Reagents and materials

[0101] (1) Reagents: DMEM medium (a medium containing various amino acids and glucose), fetal bovine serum, DMSO (dimethyl sulfoxide), superoxide dismutase (SOD) activity detection kit (brand: Solarbio, catalog number: BC0175).

[0102] (2) Instruments and equipment: Multifunctional microplate reader and electronic balance.

[0103] 4. Experimental steps:

[0104] (1) Cell culture: Prepare a cell suspension 24 h before the test. Inoculate the suspension of logarithmically growing HACAT cells (human immortalized keratinocytes) into a 96-well cell culture plate, with 5000 cells per well, and culture for 24 h. Culture conditions: 37°C; 5% CO2; saturated humidity.

[0105] (2) Exposure: Discard the culture medium in the wells, add 100 μL of the test sample to each well, and culture for 24 h. Set up a normal cell group Control(-L), an ultraviolet stress group Control(+L), a positive control group, and a test sample group respectively.

[0106] (3) Sample collection: Collect the HACAT cells after co-incubation with the test sample, perform ultrasonic disruption, centrifuge to obtain the supernatant, and use it fresh or store it at -20 °C for later use.

[0107] (4) Determination: Add the corresponding reagents to the 96-well plate in the order shown in Table 7, and operate according to the instructions of the superoxide dismutase (SOD) activity detection kit. Measure the absorbance value of each well at 560 nm using an enzyme-linked immunosorbent assay (ELISA) reader.

[0108] Table 7 Sample Liquor Addition List

[0109] Reagent A - Measurement tube A - Control tube A - Blank tube 1 A - Blank tube 2 Sample (μL) 20 20 / / Reagent 1 (μL) 45 45 45 45 Reagent 2 working solution (μL) 20 / 20 / Reagent 3 (μL) 35 35 35 35 Deionized water 70 90 90 110 Reagent 4 working solution (μL) 10 10 10 10

[0110] 5. The SOD activity calculation formula is as follows (2)

[0111]

[0112] In formula (2),

[0113] ΔA blank: The difference in absorbance between A-blank tube 1 and A-blank tube 2;

[0114] ΔA measurement: The difference in absorbance between A-measurement tube and A-control tube;

[0115] W: Sample mass;

[0116] F: Sample dilution factor.

[0117] Table 8

[0118]

[0119]

[0120] As shown in Table 8, verified by the antioxidant experiment, all the application examples of the present invention have good effects of scavenging free radicals and antioxidant. Among them, the emulsion prepared with the Leucojum aestivum tea fermentation composition of Example 9 of the present invention has the best antioxidant effect. The increase in the content of the black tea fermentation filtrate helps its synergistic effect with other components and improves the antioxidant efficacy of the Leucojum aestivum tea fermentation composition. If astaxanthin is used to replace the Leucojum aestivum bulb extract, its SOD activity is only 31.22 ± 2.04 U / g, which is much lower than that of Example 9 and even lower than that of Application Comparative Example 3 without astaxanthin or Leucojum aestivum bulb extract.

[0121] Test Example 3: Repair Barrier Efficacy Test (Human)

[0122] 1. Test samples: Emulsions prepared according to Application Examples 1-11 and Application Comparative Examples 1-10.

[0123] 2. Subjects: A total of 32 people, all 32 being female, aged 38 to 58 years old, with an average age of 50.44 ± 5.42 years old, meeting the voluntary inclusion criteria for subjects.

[0124] 3. Test instruments:

[0125] Skin moisture loss tester TM 300 (Courage+Khazaka, Germany)

[0126] 4. Principle of repairing the skin barrier: Use the Tewameter to measure 3 times and take the average value.

[0127] (1) Parameter explanation: Based on Fick's diffusion law, the water vapor pressure formed on the skin surface is detected by an internal capacitance sensor, and thus the trans-epidermal water loss (TEWL) is calculated. The smaller the TEWL value, the better the skin barrier.

[0128] (2) Result determination: After using the product, if the TEWL value in the test area shows a significant decrease, it indicates that the tested product has the effect of repairing the skin barrier.

[0129] 5. The test method for the effect of repairing the skin barrier is as follows:

[0130] Single-center open trial. Through 30 (or more) subjects using the product for 4 weeks, with self-control before and after, and using the instrument test method, the trans-epidermal water loss rate before use and after 4 weeks of use is measured to verify the effect of the product in repairing the skin barrier.

[0131] Table 9

[0132]

[0133]

[0134] As shown in Table 9, verified by the experiment on the effect of repairing the skin barrier, all the examples of the present invention have a good effect of repairing the skin barrier. Among them, the emulsion prepared with the Leucojum aestivum tea fermentation composition of Example 9 of the present invention has the best effect of repairing the skin barrier. The increase in the content of the black tea fermentation filtrate helps its synergistic effect with other components, can ensure the reduction of the skin moisture loss rate, and improve the effect of the Leucojum aestivum tea fermentation composition in repairing the skin barrier.

[0135] Test Example 4: Test for inhibiting tyrosinase activity

[0136] 1. Test samples: Emulsions prepared according to Application Examples 1-11 and Application Comparative Examples 1-10.

[0137] 2. Experimental principle: Tyrosinase is the rate-limiting enzyme in the melanin synthesis pathway, mainly affecting melanin production by influencing the conversion of tyrosine to dopa and the oxidation of dopa to dopaquinone. The principle is that tyrosine or dopa is converted to dopaquinone under the action of tyrosinase. This reaction is a color reaction, which is determined by colorimetry to judge the inhibition rate of different brightening agents on tyrosinase activity.

[0138] 3. Reagents and materials:

[0139] (1) Prepare 0.05 mol / L PBS buffer (phosphate buffered saline, pH = 6.8):

[0140] Solution A: Weigh 7.099 g of disodium hydrogen phosphate and add distilled water to 1000 mL to obtain 0.05 mol / L Na2HPO4 solution;

[0141] Solution B: Weigh 6.803 g of potassium dihydrogen phosphate and add distilled water to 1000 mL to obtain 0.05 mol / L KH2PO4 solution;

[0142] Take 50 mL of Solution A and 50 mL of Solution B and mix them to obtain 0.05 mol / L PBS buffer (pH = 6.8).

[0143] (2) Prepare tyrosinase solution: Use the PBS buffer in step (1) to prepare tyrosinase (25 kU) into 100 U / mL, and prepare it immediately before use.

[0144] (3) Prepare dopa solution: Weigh 0.04 g of dopa, dissolve it in 40 mL of the PBS buffer in step (1), and store it in the dark.

[0145] (4) Prepare α-arbutin: Dilute α-arbutin to 100 μM with the PBS buffer in step (1).

[0146] 4. Instruments and equipment: Analytical balance (accurate to 0.0001 g), 1 L volumetric flask, 50 mL measuring cylinder, 1000 μL pipette, constant temperature water bath, UV-visible spectrophotometer.

[0147] 5. Measurement steps

[0148] Prepare the test solution according to Table 10, and sequentially add the test sample, PBS buffer and tyrosinase solution into the test tube.

[0149] Table 10 Composition of reaction solution (mL)

[0150]

[0151] After preparing the test solution, place the test tube in a 37 °C water bath for 10 min; then, add 2 mL of dopa solution, and after reacting for 5 min, measure the absorbance at 475 nm. The absolute difference between the two independent determination results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean. Calculate the tyrosinase inhibition rate (%) according to formula (2).

[0152] Tyrosinase inhibition rate (%) = [1 - (OD C - OD D ) / (OD A - OD B )] × 100% Formula (3)

[0153] In formula (3),

[0154] OD A : Absorbance value of the enzyme solution control group;

[0155] OD B : Absorbance value of the blank control group of the enzyme solution;

[0156] OD C : Absorbance value of the sample group;

[0157] OD D : Absorbance value of the blank control of the sample group.

[0158] Table 11

[0159]

[0160]

[0161] The test results are shown in Table 11. Compared with the comparative example, the snowflake leucojum tea fermentation composition prepared in the embodiment of the present invention has significant tyrosinase inhibitory activity, that is, it has a good brightening and whitening effect. Among them, the snowflake leucojum tea fermentation composition in Example 9 has the best effect, inhibiting tyrosinase activity, inhibiting the production of melanin, and promoting the metabolism of melanin to achieve the whitening effect.

[0162] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A Leucophyllum australis tea fermentation composition, characterized in that: The invention comprises the following components in parts by weight: 3-15 parts of black tea fermentation filtrate, 0.01-0.6 parts of theanine and 0.5-3 parts of Leucophyllum aestivum bulb extract.

2. The Leucophyllum australis tea fermentation composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 10 parts of black tea fermentation filtrate, 0.1 parts of theanine and 1 part of Leucophyllum aestivum bulb extract.

3. Use of the Leucophyllum aestivum tea fermentation composition according to claim 1 or 2 in the preparation of antioxidant products.

4. Use of the Leucophyllum aestivum tea ferment composition according to claim 1 or 2 in the preparation of skin barrier repair products.

5. Use of the Leucophyllum aestivum tea fermentation composition according to claim 1 or 2 in the preparation of skin whitening products.

6. A cosmetic, characterized in that: The cosmetic contains the Leucophyllum aestivum tea fermentation composition according to claim 1 or 2.

7. The cosmetic according to claim 5, characterized in that: The cosmetics also contain cosmetic accessories.

8. The cosmetic according to claim 6 or 7, characterized in that: Cosmetic excipients include at least one of an emollient, a dispersant, a humectant, a preservative, an emulsion stabilizer, a thickener, a soothing agent, a chelating agent, a pH adjuster and a solvent.

9. The cosmetic according to claim 8, characterized in that: The thickener includes acrylates / C10-30 alkyl acrylate cross-linked polymers and / or sodium acrylate / sodium acryloyldimethyl taurate copolymers; the chelating agent includes disodium EDTA; the moisturizer includes at least one of glycerin, sodium hyaluronate, butylene glycol and 1,3-propylene glycol; the soothing agent includes allantoin; the preservative includes p-hydroxyacetophenone and / or phenoxyethanol; the emollient includes at least one of caprylic / decanoic acid triglyceride, polydimethylsiloxane and dicaprylyl carbonate; the pH adjuster includes aminomethyl propanol; the emulsion stabilizer includes C14-22 alcohol C12-20 alkyl glucoside; and the solvent includes water.

10. The method for preparing the cosmetic according to any one of claims 5 to 9, characterized in that: The following steps are involved: S1: Stir the phase B raw material at 500-800 rpm and adjust the temperature to 80-85°C; S2: Mix the raw material of phase A with the raw material of phase B processed in step S1, and homogenize at 2200rpm for 2-3min; keep stirring at 800-1200rpm, adjust the temperature to 80-85°C, keep stirring for 15min, and stir to dissolve evenly; S3: Mix the C phase raw material with the product treated in step S2, homogenize at 2500 rpm for 2-4 min, and stir evenly at 800 rpm; S4: Adjust the temperature of the product after the treatment in step S3 to 55°C, add the raw material of phase D, stir at 800-1000 rpm for 3-5 minutes, and stir to dissolve evenly; adjust the temperature to 45°C, add the raw material of phase E, stir at 600-1000 rpm to dissolve evenly; adjust the temperature to 38°C, filter, and obtain the cosmetic; The phase A raw material includes at least one of acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, disodium EDTA, allantoin, glycerin, sodium hyaluronate, polydimethylsiloxane and water; The phase B raw material includes at least one of dicaprylyl carbonate, caprylic / caprylic triglyceride and C14-22 alcohol C12-20 alkyl glucoside; The C phase raw material includes at least one of sodium acrylate / sodium acryloyl dimethyl taurate copolymer, aminomethyl propanol and water; The phase D raw material includes butanediol and / or p-hydroxyacetophenone; The E-phase raw material includes the Leucophyllum aestivum tea fermentation composition according to claim 1 or 2, and also includes at least one of 1,3-propylene glycol, phenoxyethanol and water.

Images