Crushing and enzymolysis process for obtaining silkworm chrysalis ACE inhibitory peptide

Abstract

The invention discloses a crushing and enzymolysis process for obtaining silkworm chrysalis ACE inhibitory peptide, and belongs to the technical field of protein peptide enzymolysis. Degreased silkworm chrysalis meal is used as a raw material, a raw material stirring and air filtering structure is optimized based on the enzymolysis principle of crushing and stirring the raw material, and in the water phase dissolving process, the enzymolysis efficiency is improved; the degreased silkworm chrysalis meal is efficiently dispersed downwards along the conical surface on the flow guide and gas filtration assembly by utilizing the plurality of liquid injection pipes, and the formed suspension liquid is preheated in the preheating space, so that the enzymolysis reaction of subsequent enzymolysis liquid is favorably improved, and in the enzymolysis reaction process, the stirring assembly periodically rotates, and the stirring efficiency is improved. Multi-point stirring exhaust and top scraping actions are set, so that waste gas in enzymatic hydrolysate is comprehensively and effectively collected, the waste gas is subjected to internal and external dual filtration through a filter disc and a filter material, and viscous solid substances intercepted on the filter disc fall into an enzymolysis space again along with scraping of a stirring assembly to be subjected to an enzymolysis reaction.

Description

Technical Field

[0001] The present invention relates to the technical field of protein peptide enzymolysis, and more specifically, to a comminution enzymolysis process for obtaining silkworm pupa ACE inhibitory peptide. Background Art

[0002] Alcoholic liver damage (ALD) is a liver disease caused by long-term or excessive alcohol consumption and is the main cause of non-viral liver damage. The renin-angiotensin system (RAS) is an important humoral regulatory system in the human body. When the liver is stimulated by pathogenic factors such as alcohol, RAS is activated, leading to damage to the structure and function of the liver. Functional peptides with angiotensin-converting enzyme (ACE) inhibitory activity (ACE inhibitory peptides) down-regulate the expression of RAS and reduce the degree of liver damage caused by alcohol.

[0003] Silkworm pupa is the main by-product of silk, rich in high-quality protein and having ACE inhibitory peptides with high inhibitory activity. ACE inhibitory peptides are an effective ingredient, but the development and utilization rate of silkworm pupa is low, resulting in a great waste of resources.

[0004] In the process of preparing silkworm pupa ACE inhibitory peptide, it is necessary to comminute and enzymolyze silkworm pupa. The enzymolysis device used only places the pre-comminuted collagen peptide raw material in an independent reaction tank through an independent reaction tank. For example, an enzymolysis mixing device for producing small molecule bone protein peptides disclosed in patent number CN117778180B heats the protein peptide raw material and protease solvent by an internal heating component and stirs them by an internal stirring component.

[0005] However, during the enzymolysis of silkworm pupa, exhaust gases of various components will be generated, including unreacted raw materials, enzymolysis products, and some organic substances that may volatilize. Some fine reaction viscous particles will be discharged together with the exhaust gas. On the one hand, it causes a large amount of solid substances to be doped in the exhaust gas, affecting subsequent exhaust gas treatment. On the other hand, the exhaust gas carrying unreacted substances is discharged, affecting the enzymolysis effect.

[0006] Therefore, we propose a comminution enzymolysis process for obtaining silkworm pupa ACE inhibitory peptide. Summary of the Invention

[0007] The purpose of the present invention is to solve existing practical problems. Compared with the prior art, a comminution enzymolysis process for obtaining silkworm pupa ACE inhibitory peptide is provided, optimizing the raw material shunt stirring structure and adding an exhaust gas filtration structure to effectively filter and treat the exhaust gas generated during the enzymolysis process.

[0008] The purpose of the present invention can be achieved by the following technical solutions: A comminution enzymolysis process for obtaining silkworm pupa ACE inhibitory peptide, comprising the following steps:

[0009] Step 1: Pretreatment of raw materials. Using silkworm pupae as raw materials, they are pre-treated by degreasing and drying, and then put into a pulverizing device for pulverizing and sieving to obtain defatted silkworm pupa powder.

[0010] Step 2: Preheating treatment of the aqueous phase. The defatted silkworm pupa powder is introduced into the preheating space of the enzymatic hydrolysis tank, and an appropriate amount of aqueous solution is injected through the liquid injection pipe for aqueous phase dissolution. It is distributed and diffused outward by the diversion and air filtration component to obtain a silkworm pupa suspension, and preheating treatment is carried out.

[0011] Step 3: Enzymatic hydrolysis treatment. The silkworm pupa suspension is introduced into the enzymatic hydrolysis space of the enzymatic hydrolysis tank, and protease is added through the feeding pipe to form an enzymatic hydrolysis solution. The stirring component installed in the enzymatic hydrolysis tank is rotated by the driving mechanism to mix and stir the enzymatic hydrolysis solution.

[0012] In this process, the stirring component rotates periodically, and multi-point stirring and exhaust as well as top scraping actions are set. Specifically, the generated waste gas is led upward and intercepted and filtered by the diversion and air filtration component to complete the solid-gas separation action. Subsequently, the waste gas is introduced into the water bath inner cylinder of the stirring component for water washing and cooling and then discharged.

[0013] Step 4: Enzyme inactivation treatment. The temperature is raised to terminate the enzymatic hydrolysis reaction to obtain an enzymatic hydrolysis product.

[0014] Step 5: Separation and purification treatment. The enzymatic hydrolysis product is discharged through the discharge pipe, and then high-purity silkworm pupa ACE inhibitory peptide is obtained through separation and purification.

[0015] Furthermore, the pulverizing device includes a pulverizing box body fixedly installed at the top end of the enzymatic hydrolysis tank. The pulverizing box body includes a conveying long pipe and a pulverizing cylinder. A pulverizing cutter extending into the pulverizing cylinder is rotatably installed inside the conveying long pipe. A blanking port is opened at the bottom end of the pulverizing cylinder, and a storage hopper penetrating to the top of the preheating space of the enzymatic hydrolysis tank is fixed at the bottom end of the blanking port.

[0016] Furthermore, the inner diameter of the pulverizing cylinder is larger than the inner diameter of the conveying long pipe. An overscreen cylinder corresponding to the position of the blanking port and fixedly connected to the end of the pulverizing cutter is rotatably installed inside the pulverizing cylinder.

[0017] Furthermore, the stirring component includes a rotating base installed at the inner bottom of the enzymatic hydrolysis tank and provided with a discharge pipe. An inner water bath cylinder coaxially arranged with the rotating base and having a discharge space reserved between it and the discharge pipe is installed at the upper end of the rotating base. A plurality of ventilation plates fixedly connected to the upper end surface of the rotating base are annularly arranged at the outer end of the inner water bath cylinder. A plurality of air filtration rods are distributed in the vertical direction on the ventilation plates, and a scraping strip in contact with the bottom end surface of the diversion and air filtration component is fixed at the top end of each ventilation plate.

[0018] Further, an air vent groove that communicates with a plurality of air filter rods and has an upper end opening is formed inside the air vent plate. A plurality of overflow holes are formed in the end wall of each air filter rod, and a waterproof and breathable membrane is coated on the outer surface of each air filter rod.

[0019] Further, the diversion and air filtration assembly includes a conical outer shell and a filter disc fixed to its bottom end surface and fixedly connected to the upper end wall of the inner water bath cylinder. The conical outer shell is filled with filter material.

[0020] Further, air cavities that communicate with the inside of the inner water bath cylinder are reserved in both the filter material and the middle of the filter disc. A second water inlet pipe and an exhaust pipe that penetrate below the rotating base are externally connected to the bottom end of the inner water bath cylinder.

[0021] Further, a blanking gap is reserved between the outer wall of the conical outer shell and the inner wall of the enzymolysis tank. An electromagnetic ring located above the diversion and air filtration assembly is fixedly embedded on the inner wall of the preheating space. A magnetic sealing ring adapted to the blanking gap is installed on the electromagnetic ring through a plurality of guide rods for lifting and lowering.

[0022] Compared with the prior art, the advantages of the present invention are as follows:

[0023] (1) In this solution, defatted silkworm pupa powder is used as the raw material. Based on the enzymolysis principle of raw material crushing and stirring, the raw material stirring and air filtration structure is optimized. During the water-phase dissolution process, a plurality of liquid injection pipes are used to efficiently disperse the defatted silkworm pupa powder downward along the conical surface of the diversion and air filtration assembly, and the formed suspension is preheated in the preheating space, which is beneficial to improving the enzymolysis reaction of the subsequent enzymolysis solution. During the enzymolysis reaction process, the stirring assembly rotates periodically, and multi-point stirring and exhaust as well as top scraping actions are set to comprehensively and effectively collect the waste gas inside the enzymolysis solution. The waste gas is filtered twice inside and outside by the filter disc and the filter material, and the viscous solid substances intercepted on the filter disc fall back into the enzymolysis space with the scraping of the stirring assembly for enzymolysis reaction.

[0024] (2) In this solution, the stirring assembly also rotates periodically. A plurality of air filter rods are used to stir the enzymolysis solution. The gas generated inside the enzymolysis solution is discharged upward through the plurality of air filter rods and the air vent plate, and the viscous solid substances on the filter disc are scraped downward by the rotating scraping strip. On the one hand, this is beneficial to avoiding the blockage of the filter disc. On the other hand, the viscous solid substances scraped downward then undergo enzymolysis reaction, improving the enzymolysis effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is the process flow chart of the present invention;

[0026] Figure 2 is the external structure schematic diagram of the crushing and enzymolysis equipment of the present invention;

[0027] Figure 3Another external structure schematic diagram of the crushing and enzymatic hydrolysis equipment of the present invention;

[0028] Figure 4 Internal sectional view of the crushing and enzymatic hydrolysis equipment of the present invention;

[0029] Figure 5 Structure schematic diagram of the crushing box of the present invention;

[0030] Figure 6 Exploded view of the crushing box of the present invention;

[0031] Figure 7 Sectional view of the crushing box of the present invention;

[0032] Figure 8 Top view of the joint of the stirring component and the diversion and air filtration component of the present invention;

[0033] Figure 9 Bottom view of the joint of the stirring component and the diversion and air filtration component of the present invention;

[0034] Figure 10 Exploded view of the joint of the stirring component and the diversion and air filtration component of the present invention;

[0035] Figure 11 Cross-sectional view of the crushing and enzymatic hydrolysis equipment of the present invention.

[0036] Explanation of the reference numerals in the figure:

[0037] 1. Enzymatic hydrolysis tank; 2. Water bath outer shell; 201. First water inlet pipe; 3. Crushing box; 31. Conveyor long pipe; 32. Crushing cylinder; 321. Material dropping port; 4. First driving motor; 5. Storage hopper; 6. Sieving cylinder; 7. Crushing cutter; 8. Rotary base; 9. Inner water bath cylinder; 901. Second water inlet pipe; 902. Exhaust pipe; 10. Diversion and air filtration component; 101. Conical outer shell; 102. Filter disc; 103. Filter material; 11. Rotary sleeve; 12. Tooth ring; 13. Gear; 14. Second driving motor; 15. Diversion cover; 16. Liquid injection pipe; 17. Feeding pipe; 18. Ventilation plate; 19. Air filtration rod; 20. Scraping strip; 21. Electromagnetic ring; 22. Magnetic sealing ring; 23. Guide rod. Detailed implementation manners

[0038] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0039] Example 1: The present invention discloses a pulverization and enzymatic hydrolysis process for obtaining silkworm pupa ACE inhibitory peptide. Please refer to Figures 1 - 4 , which includes the following steps:

[0040] Step 1: Raw material pretreatment. Using silkworm pupa as the raw material, it is pre-treated by degreasing and drying, and then put into a pulverizing device for pulverization and sieving to obtain degreased silkworm pupa powder.

[0041] Step 2: Aqueous phase preheating treatment. The degreased silkworm pupa powder is introduced into the preheating space in enzymatic hydrolysis tank 1, and an appropriate amount of aqueous solution is injected through liquid injection pipe 16 for aqueous phase dissolution. It is distributed and diffused by the conical end face on the diversion and filtration component 10 to obtain a silkworm pupa suspension, and the silkworm pupa suspension is preheated.

[0042] Step 3: Enzymatic hydrolysis treatment. The preheated silkworm pupa suspension is introduced into the enzymatic hydrolysis space in enzymatic hydrolysis tank 1, and an appropriate amount of protease is added through feeding pipe 17 to form an enzymatic hydrolysate. The driving mechanism rotates to drive the stirring component installed in enzymatic hydrolysis tank 1 to mix and stir the enzymatic hydrolysate, and an enzymatic hydrolysis reaction is carried out in a water bath at 45 - 55 °C for 3 - 6 hours.

[0043] In this process, the exhausted gas is led upward and intercepted and filtered by the diversion and filtration component 10. The viscous solid substances adhere to the filter disk 102 to complete the solid-gas separation action. The gas enters the interior of the diversion and filtration component 10, is filtered by the filter material 103, and is introduced into the water bath inner cylinder 9 of the stirring component for water washing and cooling, and finally discharged through the exhaust pipe 902.

[0044] In the exhaust gas filtration treatment, with the periodic rotation of the stirring component, multi-point stirring exhaust and top scraping actions are set. Specifically: multiple filter gas rods 19 are used to stir the enzymatic hydrolysate, and the gas generated inside the enzymatic hydrolysate is discharged upward through the multiple filter gas rods 19 and the ventilation plate 18, and the viscous solid substances on the filter disk 102 are scraped downward by the rotating scraping strip 20. The downward-scraped viscous solid substances then undergo an enzymatic hydrolysis reaction.

[0045] Step 4: Enzyme inactivation treatment. The temperature is raised to 90 - 100 °C to inactivate the protease and terminate the enzymatic hydrolysis reaction to obtain an enzymatic hydrolysis product.

[0046] Step 5: Separation and purification treatment. The enzymatic hydrolysis product is discharged through the discharge pipe, and then the enzymatic hydrolysis product is separated and purified by methods such as filtration, centrifugation, ultrafiltration, gel chromatography, and ion exchange chromatography to remove small molecule substances such as unhydrolyzed substrates, impurities, and salts, and obtain high-purity silkworm pupa ACE inhibitory peptide.

[0047] Example 2: Regarding the pulverization and enzymatic hydrolysis process disclosed in Example 1, the following pulverization and enzymatic hydrolysis equipment is involved. Please refer to Figures 2 - 4 :

[0048] An external installation of the enzymatic hydrolysis tank 1 is provided with a water bath housing 2 that forms a closed water bath space between its outer wall. An inlet pipe 201 is externally connected to the water bath housing 2. The height of the water bath space is greater than or equal to the total height of the preheating space and the enzymatic hydrolysis space. A heating device is installed in the water bath space to heat the water in the water bath space to meet the water bath requirement at a certain temperature.

[0049] Please refer to Figures 4 - 7 , the crushing device includes a crushing box body 3 fixedly installed at the top of the enzymatic hydrolysis tank 1. The crushing box body 3 includes a conveying long pipe 31 and a crushing cylinder 32. At the upper end of one side of the conveying long pipe 31 away from the crushing cylinder 32, there is a feed hopper. A crushing cutter 7 extending into the crushing cylinder 32 is rotationally embedded and installed inside the conveying long pipe 31. A driving motor 4 for rotating the crushing cutter 7 is fixed to the outer end of the conveying long pipe 31. A material dropping port 321 is opened at the bottom end of the crushing cylinder 32, and a storage hopper 5 penetrating through the top of the preheating space of the enzymatic hydrolysis tank 1 is fixed at the bottom end of the material dropping port 321. The inner diameter of the crushing cylinder 32 is larger than the inner diameter of the conveying long pipe 31. A sieving cylinder 6 corresponding to the position of the material dropping port 321 and fixedly connected to the end of the crushing cutter 7 is rotationally embedded and installed inside the crushing cylinder 32;

[0050] The crushing cutter 7 includes a rotating shaft penetrating between the conveying long pipe 31 and the crushing cylinder 32. A spiral blade located inside the conveying long pipe 31 and a crushing blade located inside the crushing cylinder 32 are fixedly installed on the rotating shaft. Multiple groups of crushing blades are provided;

[0051] The dried defatted silkworm pupa raw material is introduced into the inside of the conveying long pipe 31 from the feed hopper. During the process of being crushed by the spiral blade, the raw material is synchronously conveyed into the crushing cylinder 32 and falls into the sieving cylinder 6, and then is further crushed by the crushing blades inside the crushing cylinder 32;

[0052] During the secondary crushing process, the sieving cylinder 6 rotates together with the rotating shaft to perform sieving on the crushed silkworm pupa powder. The silkworm pupa powder meeting the crushing requirements falls into the storage hopper 5 through the filter holes on the sieving cylinder 6 for storage. The crushing device is directly placed on the top of the enzymatic hydrolysis tank 1. The crushed silkworm pupa powder can directly fall into the preheating space inside the enzymatic hydrolysis tank 1 for aqueous phase dissolution, or after all the crushing is completed, the material dropping valve at the bottom of the storage hopper 5 can be opened for unified aqueous phase dissolution.

[0053] Please refer to Figure 4 and Figures 8 - 11, the stirring assembly includes a rotating base 8 installed at the inner bottom of the enzymatic hydrolysis tank 1 and equipped with a discharge pipe. At the upper end of the rotating base 8, a water bath inner cylinder 9 coaxially arranged with it and having a discharge space reserved between it and the discharge pipe is installed. A plurality of ventilation plates 18 fixedly connected to the upper end surface of the rotating base 8 are annularly arranged at the outer end of the water bath inner cylinder 9. A plurality of air filtering rods 19 are distributed in the vertical direction on the ventilation plates 18. And a scraping strip 20 in contact with the bottom end surface of the diversion and air filtering assembly 10 is fixed to the top end of each ventilation plate 18. An air ventilation groove communicating with a plurality of air filtering rods 19 and having an upper opening is formed inside the ventilation plate 18. A plurality of overflow holes are formed in the end wall of each air filtering rod 19, and a waterproof and breathable membrane is coated on the outer surface of each air filtering rod 19;

[0054] The driving mechanism includes a rotating sleeve 11 rotatably installed at the inner bottom of the enzymatic hydrolysis tank 1 and threadedly sleeved on the outer wall of the rotating base 8. A toothed ring 12 is embedded and installed on the outer end wall of the rotating sleeve 11. A gear 13 meshing with the toothed ring 12 is rotatably installed at the bottom outside of the water bath housing 2 through a driving motor two 14.

[0055] The diversion and air filtering assembly 10 is installed above the stirring assembly. The diversion and air filtering assembly 10 is used to divide the internal space of the enzymatic hydrolysis tank 1 into a preheating space above and an enzymatic hydrolysis space below. The diversion and air filtering assembly 10 includes a conical housing 101 and a filtering disc 102 fixed to its bottom end surface and fixedly connected to the upper end wall of the water bath inner cylinder 9;

[0056] The conical housing 101 is a hollow conical structure that is narrow at the top and wide at the bottom. The inside of the conical housing 101 is filled with a filter material 103. The filter material 103 can be selected from activated carbon or molecular sieve materials. Air cavities communicating with the inside of the water bath inner cylinder 9 are reserved in the middle of the filter material 103 and the filtering disc 102. A second water inlet pipe 901 and an exhaust pipe 902 passing through below the rotating base 8 are externally connected to the bottom end of the water bath inner cylinder 9. Water is introduced into the water bath inner cylinder 9 through the second water inlet pipe 901. Heating equipment is also installed inside the water bath inner cylinder 9 for heating the water body to meet the water bath requirements at a certain temperature;

[0057] Please refer to Figure 4 and Figure 11, a deflector 15 is fixedly installed at the top in the preheating space and is located at the upper end of the conical outer shell 101. A diversion space is reserved between the deflector 15 and the upper end face of the conical outer shell 101. A plurality of liquid injection pipes 16 are provided, and the inner ends of the plurality of liquid injection pipes 16 are evenly distributed inside the deflector 15 with the lower outlet of the storage hopper 5 as the center. A blanking gap is reserved between the outer wall of the conical outer shell 101 and the inner wall of the enzymolysis tank 1. An electromagnetic ring 21 is fixedly embedded on the inner wall of the preheating space and is located above the diversion and air filtering assembly 10. A magnetic sealing ring 22 adapted to the blanking gap is installed on the electromagnetic ring 21 through a plurality of guide rods 23. The magnetic sealing ring 22 is made of a high-sealing material and has a certain weight. In the state where the electromagnetic ring 21 is disconnected, the magnetic sealing ring 22 blocks the blanking gap under the action of gravity;

[0058] In the initial state, the magnetic sealing ring 22 blocks the blanking gap, realizing the independent sealing treatment between the preheating space and the enzymolysis space. During the aqueous phase preheating process, the blanking valve below the storage hopper 5 is opened, and at the same time, a plurality of liquid injection pipes 16 are opened. The plurality of liquid injection pipes 16 spray aqueous solution towards the top of the conical outer shell 101, and the degreased silkworm pupa powder is efficiently dispersed downward along the conical surface of the diversion and air filtering assembly 10 by the plurality of liquid injection pipes 16, and the formed suspension is preheated in the preheating space. In order to improve the preheating effect, an electric heating tube can be added to the top of the preheating space, and combined with the water bath heating of the water bath outer shell 2, the preheating temperature is increased.

[0059] At the same time, the driving mechanism is used to drive the rotating base 8, the inner water bath cylinder 9 and the diversion and air filtering assembly 10 to rotate as a whole, effectively shaking the suspension in the preheating space and improving the preheating uniformity. After the preheating is completed, the electromagnetic ring 21 is started, and the magnetic attraction of the electromagnetic ring 21 on the magnetic sealing ring 22 is used to open the blanking gap between the conical outer shell 101 and the inner wall of the enzymolysis tank 1. At this time, the preheated suspension falls into the lower enzymolysis space through the blanking gap. Subsequently, 221 is closed, so that the magnetic sealing ring 22 resets to the blanking gap again. At this time, the enzymolysis space is an independent closed space.

[0060] During the enzymolysis process, an appropriate amount of protease is introduced into the enzymolysis space through the feeding pipe 17. One or more feeding pipes 17 can be provided. The stirring assembly continuously rotates to stir the mixture formed by the protease and the suspension to form an enzymolysis solution. The height of the enzymolysis solution does not exceed the opening at the top of the ventilation plate 18, and an exhaust space is reserved between the top surface of the enzymolysis solution and the bottom end of the diversion and air filtering assembly 10. Under the dual water bath action of the inner water bath cylinder 9 and the water bath outer shell 2, the enzymolysis reaction is carried out in a water bath at 45 - 55 °C, and the reaction time is 3 - 6 h.

[0061] In this process, the waste gas generated in the enzymatic hydrolysate is discharged upward. Multiple groups of gas filtering rods 19 with multiple overflow holes are arranged around the inner cylinder 9 of the water bath. While achieving sufficient stirring, it is conducive to guiding the waste gas inside the enzymatic hydrolysate through the gas filtering rods 19 distributed in multiple places into the ventilation plate 18 and discharging it upward through the ventilation plate 18, ensuring that the waste gas is comprehensively and effectively collected. The waste gas discharged upward is distributed at the bottom of the diversion gas filtering assembly 10 and intercepted and filtered by the diversion gas filtering assembly 10. The viscous particulate matter in the waste gas adheres to the filter disc 102, completing the solid-gas separation action.

[0062] The side cross-section of the scraping bar 20 is a trapezoidal structure that is narrow at the top and wide at the bottom. During the rotation of the inner cylinder 9 of the water bath with the scraping bar 20, it is used to scrape the viscous particulate matter on the filter disc 102, and the viscous particulate matter falls back into the enzymatic hydrolysis space for enzymatic hydrolysis reaction. In addition, the top cross-section of 18 is a trapezoidal structure that is narrow on the outside and wide on the inside, which is conducive to the enzymatic hydrolysis products being discharged outward from the bottom end surface of the rotating base 8 towards the discharge pipe during discharging.

[0063] The waste gas enters the inside of the diversion gas filtering assembly 10, is filtered by the filter material 103, and is guided downward into the inner cylinder 9 of the water bath under the action of gas cavity diversion. The water in the inner cylinder 9 of the water bath washes and cools the waste gas to remove soluble substances, and the finally treated gas is discharged through the exhaust pipe 902. According to the external discharge requirements, the gas can be connected to external waste gas treatment equipment for subsequent further purification treatment.

[0064] When the enzymatic hydrolysis reaction is approaching completion, the generation amount of waste gas will gradually decrease and tend to be stable because most of the substrates have been decomposed, and the substances that can continue to react to produce gas gradually decrease. Therefore, a gas flow meter is set at the exhaust pipe 902 to monitor the gas discharge amount, and the enzymatic hydrolysis reaction can be supervised.

[0065] After the reaction is completed, the water body that has acted in the inner cylinder 9 of the water bath is discharged through the second water inlet pipe 901. This water body needs to be subjected to subsequent filtration treatment. The stirring assembly and the rotating sleeve 11 are installed in a threaded manner, which facilitates the downward detachment of the stirring assembly together with the diversion gas filtering assembly 10 from the enzymatic hydrolysis tank 1 for subsequent in-depth cleaning.

[0066] In summary: The present invention uses defatted silkworm pupa powder as the raw material. Based on the enzymatic hydrolysis principle of raw material crushing and stirring, it optimizes the raw material stirring and gas filtering structure. During the water phase dissolution process, multiple injection pipes are used to efficiently disperse the defatted silkworm pupa powder downward along the upper conical surface of the diversion gas filtering assembly, and the formed suspension is preheated in the preheating space, which is conducive to improving the enzymatic hydrolysis reaction of the subsequent enzymatic hydrolysate. During the enzymatic hydrolysis reaction process, the stirring assembly rotates periodically, and multi-point stirring and exhaust as well as top scraping actions are set.

[0067] Specifically: multiple filter rods are used to stir the enzymatic hydrolysate. The gas generated inside the enzymatic hydrolysate is discharged upward through the multiple filter rods and the ventilation plate, and the waste gas is filtered both inside and outside by the filter disc and the filter material. In addition, the viscous solid substances on the filter disc are scraped downward by the rotating scraping bar. On the one hand, this is conducive to avoiding the blockage of the filter disc. On the other hand, the viscous solid substances scraped downward then undergo an enzymatic hydrolysis reaction.

[0068] The above is only a preferred specific embodiment of the present invention; however, the protection scope of the present invention is not limited thereto; any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its improved concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae, characterized in that: The following steps are involved: Step 1: Raw material pretreatment, using silkworm pupae as raw materials, pre-degreasing and drying, and then putting them into a crushing device for crushing and sieving to obtain defatted silkworm pupa powder; Step 2: preheating the aqueous phase, introducing the defatted silkworm pupa powder into the preheating space of the enzymolysis tank (1), and simultaneously injecting an appropriate amount of aqueous solution through the injection pipe (16) to dissolve the aqueous phase, distributing and diffusing it outwards through the flow guide and air filter component (10) to obtain a silkworm pupa suspension, and preheating it; Step 3: enzymatic hydrolysis treatment, introducing the silkworm pupa suspension into the enzymatic hydrolysis space of the enzymatic hydrolysis tank (1), adding protease through the injection pipe (17) to form an enzymatic hydrolysis solution, and rotating the stirring component installed in the enzymatic hydrolysis tank (1) through the driving mechanism to mix and stir the enzymatic hydrolysis solution; In this process, the stirring component rotates periodically, and multiple points of stirring exhaust and top scraping actions are set. Specifically, the generated waste gas is guided upward and intercepted and filtered by the guide filter component (10) to complete the solid-gas separation action. Then, the waste gas is introduced into the water bath inner cylinder (9) of the stirring component for water washing and cooling before being discharged; Step 4: Inactivate the enzyme by raising the temperature to terminate the enzymatic reaction and obtain the enzymatic product; Step 5: separation and purification, discharging the enzymatic hydrolysis product through a discharge pipe, and subsequently separating and purifying to obtain a high-purity silkworm pupa ACE inhibitory peptide.

2. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae according to claim 1, characterized in that: The pulverizing device comprises a pulverizing box (3) fixedly mounted on the top of an enzymolysis tank (1), the pulverizing box (3) comprising a long conveying tube (31) and a pulverizing cylinder (32), a pulverizing cutter (7) extending into the pulverizing cylinder (32) being rotatably embedded inside the long conveying tube (31), a material drop opening (321) being provided at the bottom end of the material drop opening (321), and a material storage hopper (5) penetrating to the top of the preheating space of the enzymolysis tank (1) being fixed at the bottom end of the material drop opening (321).

3. A crushing and enzymatic hydrolysis process for obtaining silkworm chrysalis ACE inhibitory peptides according to claim 2, characterized in that: The inner diameter of the crushing cylinder (32) is larger than the inner diameter of the long conveying tube (31), and a screening cylinder (6) is rotatably mounted inside the crushing cylinder (32) and is fixedly connected to the end of the crushing tool (7).

4. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae according to claim 3, characterized in that: The stirring assembly comprises a rotating base (8) installed at the bottom of the enzymatic hydrolysis tank (1) and provided with a discharge pipe, the upper end of the rotating base (8) is provided with a water bath inner cylinder (9) which is coaxially arranged with the rotating base and has a discharge space reserved between the rotating base (8) and the discharge pipe, the outer end of the water bath inner cylinder (9) is provided with a plurality of ventilation plates (18) which are fixedly connected to the upper end surface of the rotating base (8) and are arranged in an annular manner, the ventilation plates (18) are provided with a plurality of air filter rods (19) distributed in the vertical direction, and a scraper strip (20) which contacts the bottom end surface of the guide air filter assembly (10) is also fixed to the top of each ventilation plate (18).

5. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae according to claim 4, characterized in that: The ventilation plate (18) is provided with a ventilation groove which is connected to a plurality of air filter rods (19) and has an upper end opening, a plurality of overflow holes are provided on the end wall of each air filter rod (19), and the outer surface of each air filter rod (19) is covered with a waterproof breathable membrane.

6. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae according to claim 5, characterized in that: The flow guide and air filter assembly (10) comprises a conical outer shell (101) and a filter plate (102) fixed to the bottom end surface thereof and fixedly connected to the upper end wall of the water bath inner cylinder (9); the conical outer shell (101) is filled with filter material (103).

7. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae according to claim 6, characterized in that: The filter material (103) and the filter disc (102) are both provided with an air cavity in the middle thereof which is in communication with the interior of the water bath inner cylinder (9). The bottom end of the water bath inner cylinder (9) is externally connected with a second water inlet pipe (901) and an exhaust pipe (902) which penetrate to the bottom of the rotating base (8).

8. A crushing and enzymatic hydrolysis process for obtaining ACE inhibitory peptides from silkworm pupae according to claim 7, characterized in that: A material-dropping gap is reserved between the outer wall of the conical housing (101) and the inner wall of the enzymolysis tank (1); an electromagnetic ring (21) located above the air guide and filter assembly (10) is fixedly embedded on the inner wall of the preheating space; a magnetic sealing ring (22) adapted to the material-dropping gap is mounted on the electromagnetic ring (21) via a plurality of guide rods (23) for lifting.

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