Adenovirus detection test strip based on high-concentration colloidal gold and preparation method thereof

Abstract

The invention relates to an adenovirus detection test strip based on high-concentration colloidal gold and a preparation method thereof, and belongs to the technical field of adenovirus detection. The adenovirus detection test strip is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, the combination pad is loaded with an adenovirus detection antibody marked by high-concentration colloidal gold, the nitrocellulose membrane is provided with a detection line, the detection line is loaded with an adenovirus capture antibody, the particle size of the high-concentration colloidal gold is 60 nm, and the particle size of the high-concentration colloidal gold is 20 nm. And the optical density value is 100. According to the invention, the high-concentration colloidal gold is used for marking the adenovirus antibody, and an optimized marking process is combined, so that the binding efficiency of the colloidal gold and the antibody is enhanced, the detection sensitivity is improved, the problem of low sensitivity of adenovirus detection by using an immunochromatography technology in the prior art is solved, and the method has popularization value.

Description

Technical Field

[0001] The present invention relates to the technical field of adenovirus detection, and in particular to an adenovirus detection test strip based on high-concentration colloidal gold and a preparation method thereof. Background Art

[0002] Adenoviruses belong to the family of non-enveloped double-stranded DNA viruses, and their typical structure consists of 252 protein capsomeres forming an icosahedral symmetry. According to genomic differences, they can be divided into seven major subgroups A-G, covering 114 genotypes. Each type has tissue tropism differences and can cause multi-system infections such as the respiratory tract (subgroups B / C), eyes (subgroup D), and digestive tract (subgroup F). The transmission routes of this virus include droplet transmission (accounting for 82% of respiratory infections), contact with contaminated surfaces (surviving for up to 240 hours), and the fecal-oral route (the main transmission mode in gastroenteritis cases), and the transmission efficiency is significantly increased in enclosed spaces. Adenovirus infections are widespread globally, especially in crowded places (such as schools, military camps, hospitals, etc.) where explosive epidemics are likely to occur. The symptoms after infection vary from mild cold-like symptoms to severe pneumonia, conjunctivitis, and may even cause other serious complications, posing a greater threat to the health of patients. Given the harmfulness of adenovirus infections, rapid and accurate detection methods are crucial for the diagnosis, treatment, and prevention and control of diseases.

[0003] In the prior art, the detection methods for adenoviruses mainly include virus isolation and identification, morphological observation, nucleic acid detection, antigen detection, and antibody detection. Among them, virus isolation and identification, as the traditional "gold standard", observes cytopathic effects by culturing the virus, but has the disadvantages of long time consumption (7-10 days), complex operation, and low sensitivity; morphological observations such as electron microscopes can directly show the virus structure, but the resolution is insufficient and it is difficult to distinguish active infections from latent viruses. Although fluorescence microscopes can quickly locate antigens, they are limited by the sample quality and are prone to false negatives. Nucleic acid detection technologies (such as PCR, RT-qPCR) have become the mainstream methods due to their high sensitivity and specificity. In particular, real-time quantitative PCR can quickly detect the viral nucleic acids in various samples, but there are problems such as expensive equipment, being easily contaminated and resulting in false positives, and being unable to distinguish current and past infections. Antigen detection (such as ELISA, immunofluorescence) realizes rapid screening by identifying viral proteins and can give results within 30 minutes, but the sensitivity is relatively low and it is easily interfered by cross-reactions. Antibody detection can trace the infection history, but its diagnostic value is limited due to the widespread presence of past infection antibodies in the population, and it requires a comparison of paired sera, resulting in inability to be applied early. In addition, although emerging metagenomic sequencing can comprehensively analyze pathogens, the cost is too high to be popularized. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides an adenovirus detection test strip based on high-concentration colloidal gold and a preparation method thereof. The adenovirus detection test strip provided by the present invention is composed of a sample pad, a conjugate pad, a nitrocellulose membrane, an absorbent pad and a base plate. The conjugate pad is loaded with an adenovirus detection antibody labeled with high-concentration colloidal gold. A detection line is provided on the nitrocellulose membrane, and the detection line is loaded with an adenovirus capture antibody. The particle size of the high-concentration colloidal gold is 60 nm, and the optical density value is 100.

[0005] The first object of the present invention is to provide an adenovirus detection test strip based on high-concentration colloidal gold. The adenovirus detection test strip is composed of a sample pad, a conjugate pad, a nitrocellulose membrane, an absorbent pad and a base plate. The conjugate pad is loaded with an adenovirus detection antibody labeled with high-concentration colloidal gold. A detection line is provided on the nitrocellulose membrane, and the detection line is loaded with an adenovirus capture antibody;

[0006] The particle size of the high-concentration colloidal gold is 60 nm, and the optical density value is 100;

[0007] The concentration of the adenovirus detection antibody is 50-80 μg / mL. After mixing and reacting with high-concentration colloidal gold for 1.2-1.5 hours, 90-100 μL of a labeling blocking solution is added and reacted for 20 minutes. The labeling blocking solution includes casein and boric acid, and the pH value of the labeling blocking solution is 8-9.

[0008] Further, the steps of using high-concentration colloidal gold to label the adenovirus detection antibody include:

[0009] (1) Take high-concentration colloidal gold, add the adenovirus detection antibody, add the labeling blocking solution after the reaction, and centrifuge to obtain the precipitate;

[0010] (2) Add a colloidal gold labeling complex solution to the precipitate. The colloidal gold labeling complex solution includes a buffer solution of 35-60 mM, casein of 0.3-0.7 wt%, sucrose of 10-30 wt%, trehalose of 2-7 wt% and a preservative of 0.02-0.06 wt%.

[0011] Further, in step (1), the pH value of the high-concentration colloidal gold is adjusted using a potassium carbonate solution. The concentration of the potassium carbonate solution is 0.01 M, and the addition amount is 15-20 μL.

[0012] Further, in step (2), the addition amount of the colloidal gold labeling complex solution is 50-80 μg / mL.

[0013] Further, the capture antibody is diluted with a coating dilution solution. The coating dilution solution includes Tris, sucrose, bovine serum albumin and a preservative. After dilution, the concentration of the capture antibody is 0.7-2.0 mg / mL.

[0014] Further, the diluted capture antibody is sprayed on the test line of the nitrocellulose membrane at a rate of 1 - 2 μL / cm.

[0015] Further, the adenovirus detection test strip further includes a sample diluent, and the sample diluent includes a buffer, casein, a surfactant, and a preservative.

[0016] The second object of the present invention is to provide a method for preparing the above-mentioned adenovirus detection test strip, the steps including pre-treating the sample pad and the conjugate pad, spraying the adenovirus detection antibody labeled with high-concentration colloidal gold on the conjugate pad, setting a test line on the nitrocellulose membrane, spraying the adenovirus capture antibody on the test line, and assembling the sample pad, the conjugate pad, the nitrocellulose membrane, and the absorbent pad.

[0017] The third object of the present invention is to provide the application of the above-mentioned adenovirus detection test strip in detecting adenovirus antigen.

[0018] The fourth object of the present invention is to provide a method for detecting adenovirus antigen, using the above-mentioned adenovirus detection colloidal gold test strip to detect a sample to be tested.

[0019] Advantages of the present invention:

[0020] The present invention uses high-concentration colloidal gold (optical density value 100, particle size 60 nm) to label the antibody, optimizes the process during the preparation of the test strip, including adjusting the antibody loading and the solution components, etc., enhances the binding efficiency of the colloidal gold and the antibody, and thus improves the detection sensitivity. Using a binding blocking solution to reduce non-specific adsorption, ensuring clear color development of the test line, and making the result interpretation more reliable. The sample diluent contains a surfactant and casein, which can promote the rapid diffusion of the sample. Combining with the chromatography characteristics of the colloidal gold test strip, the detection can be completed within 10 - 15 minutes. By precisely controlling the labeling conditions, the colloidal gold labeling step is simplified, the batch difference is reduced, the clinical application range is expanded, and the problem of low sensitivity in detecting adenovirus using immunochromatography technology in the prior art is solved, which has the value of popularization. Description of the Drawings

[0021] In order to make the content of the present invention easier to be clearly understood, the following further details the present invention according to specific embodiments of the present invention in combination with the drawings, where:

[0022] Figure 1 is a schematic structural diagram of the adenovirus detection test strip prepared by the present invention, where 1 is the sample pad, 2 is the conjugate pad, 3 is the nitrocellulose membrane, 31 is the test line, 32 is the control line, 4 is the absorbent pad, and 5 is the bottom plate;

[0023] Figure 2Detection result diagram of the adenovirus detection test strip provided by the present invention. Detailed implementation manners

[0024] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and be able to implement it, but the specific embodiments cited are not intended to limit the present invention.

[0025] Example 1: Preparation of solutions

[0026] Sample pad treatment solution, with a pH value of 8 - 9, and the components include: 40 - 80 mM Tris-HCl buffer, 0.4 - 0.8 wt% casein, 0.8 - 1.5 wt% polyvinylpyrrolidone K30, 0.8 - 1.5 wt% surfactant S9, 0.04 - 0.1 wt% Tween 20, 0.02 - 0.06 wt% ProClin300, 0.002 - 0.006 wt% heterophilic antibody blocker HBR.

[0027] Conjugate pad treatment solution, with a pH value of 8 - 9, and the components include: 40 - 80 mM Tris-HCl buffer, 0.4 - 0.8 wt% casein, 0.04 - 0.08 wt% Tween 20, 0.02 - 0.06 wt% ProClin300.

[0028] High-concentration colloidal gold solution: The colloidal gold particle size is 60 nm, and the OD value of the solution is 100.

[0029] Colloidal gold labeling complex solution, with a pH value of 8 - 9, and the components include: 35 - 60 mM Tris-HCl buffer, 0.3 - 0.7 wt% casein, 10 - 30 wt% sucrose, 2 - 7 wt% trehalose, 0.02 - 0.06 wt% ProClin300.

[0030] Labeling blocking solution, with a pH value of 8 - 9 (adjusted by sodium hydroxide), and the components include: 0.6 - 1 wt% casein, 50 - 150 mM boric acid.

[0031] Detection line coating dilution solution, with a pH value of 7 - 7.5, and the components include: 8 - 15 mM PBS buffer, 1.5 - 3 wt% sucrose, 0.02 - 0.05 wt% ProClin300.

[0032] Quality control line coating dilution solution, with a pH value of 7 - 7.5, and the components include: 8 - 15 mM PBS buffer, 1.5 - 3 wt% sucrose, 0.02 - 0.05 wt% ProClin300.

[0033] Sample diluent with a pH value of 7 - 7.5, and its components include: 50 - 150 mM Tris-HCl buffer, 0.5 - 1.2 wt% casein, 0.2 - 0.6 wt% surfactant S22, and 0.02 - 0.06 wt% ProClin300.

[0034] Example 2: Preparation of an adenovirus detection test strip

[0035] (1) Treatment of the sample pad and the conjugate pad

[0036] Soak the sample pad in the sample pad treatment solution, with the addition amount of the treatment solution being 0.05 - 0.1 mL / cm 2 , after soaking for 5 minutes (or more), perform dehydration treatment. After dehydration, dry the sample pad in an oven (set temperature: 33 - 50 °C) for more than 7 hours, and then store it sealed in an environment with a humidity ≤ 30%.

[0037] Soak the conjugate pad in the conjugate pad treatment solution, with the addition amount of the treatment solution being 0.04 - 0.08 mL / cm 2 , after soaking for 5 minutes (or more), perform dehydration treatment. After dehydration, dry the sample pad in an oven (set temperature: 33 - 50 °C) for more than 7 hours, and then store it sealed in an environment with a humidity ≤ 30%.

[0038] (2) Labeling of the detection antibody

[0039] Take 40 μL of high-concentration colloidal gold, add 45 μL of ultrapure water and 15 μL of 0.01 M potassium carbonate solution to adjust the pH value to 7.5, and dilute the absorbance to OD40. Add the mouse anti-human adenovirus detection antibody to make its concentration 50 - 80 μg / mL, mix well and react for 1.5 hours, then add 90 - 110 μL of the labeling blocking solution, mix well and react for 20 minutes. Centrifuge the prepared solution at low temperature (0 - 8 °C) and high speed (7000 - 9000 rpm) for 5 - 10 minutes. After removing the supernatant, add 70 - 80 μL of the colloidal gold labeling complex solution to the precipitate, then disperse and resuspend the precipitate to obtain the mouse anti-human adenovirus detection antibody colloidal gold labeling complex solution.

[0040] Take 40 μL of high-concentration colloidal gold, add 30 μL of ultrapure water and 30 μL of 0.01 M potassium carbonate solution to adjust the pH value to 9, and dilute the absorbance to OD40. Add the chicken IgY antibody to make its concentration 50 - 80 μg / mL, mix well and react for 1.5 hours, then add 90 - 110 μL of the labeling blocking solution, mix well and react for 20 minutes. Centrifuge the prepared solution at low temperature (0 - 8 °C) and high speed (7000 - 9000 rpm) for 5 - 10 minutes. After removing the supernatant, add 70 - 80 μL of the colloidal gold labeling complex solution to the precipitate, then disperse and resuspend the precipitate to obtain the chicken IgY antibody colloidal gold labeling complex solution.

[0041] (3) Coating of capture antibodies

[0042] Dilute the mouse anti-human adenovirus capture antibody with coating diluent to 0.7 - 1.5 mg / mL to obtain the mouse anti-human adenovirus capture antibody coating solution.

[0043] Dilute the goat anti-chicken IgY antibody with coating diluent to 0.8 - 1.2 mg / mL to obtain the goat anti-chicken IgY antibody coating solution.

[0044] (4) Assembly of test strips

[0045] Mix 70 - 80 μL of the colloidal gold-labeled complex solution of mouse anti-human adenovirus detection antibody with 20 - 25 μL of the colloidal gold-labeled complex solution of chicken IgY antibody evenly, and spray the mixture onto the surface of the pretreated conjugate pad at a uniform rate of 2.5 - 4 μL / cm. After spraying, transfer the conjugate pad to an environment at 33 - 50 °C and bake for at least 6 hours. After complete drying, store it in a sealed container, and the humidity of the storage environment needs to be strictly controlled at ≤30%.

[0046] Spray the mouse anti-human adenovirus capture antibody coating solution and the goat anti-chicken IgY antibody coating solution onto the test line and the quality control line of the nitrocellulose membrane at a spray volume of 1 - 2 μL / cm respectively. Then, place the treated nitrocellulose membrane in an environment at 30 - 50 °C and bake for at least 6 hours. After complete drying, store it in a sealed container, and the humidity of the storage environment needs to be strictly controlled at ≤30%.

[0047] Assemble the components of the test strip onto the bottom plate in the following order: Using the nitrocellulose membrane as the central positioning point, lay it flat in the middle section area of the bottom plate, and lap and bond its two ends with the absorbent pad and the conjugate pad respectively. The lap overlap width is strictly controlled within the range of 1 - 3 mm. Among them, the upper end (detection end) of the nitrocellulose membrane overlaps and connects with the end of the conjugate pad, and the lower end (sample migration terminal) overlaps and connects with the starting end of the absorbent pad. The other end of the conjugate pad is connected to the sample pad with the same overlap specification, and finally form a chromatographic path of "sample pad - conjugate pad - nitrocellulose membrane - absorbent pad". The bottom plate serves as a supporting base to carry all components and ensure the precise alignment of each functional layer.

[0048] The schematic structural diagram of the prepared adenovirus detection test strip is as Figure 1 shown, where 1 is the sample pad, 2 is the conjugate pad, 3 is the nitrocellulose membrane, 31 is the test line, 32 is the quality control line, 4 is the absorbent pad, and 5 is the bottom plate.

[0049] Example 3: Application of adenovirus detection test strip

[0050] Adenovirus test strips were prepared according to the solution provided in Example 1 and the method provided in Example 2. The adenovirus antigen standard solution was diluted to 1.4 μg / mL, 10 ng / mL, 1 ng / mL, 0.5 ng / mL, 0.2 ng / mL, and 0.1 ng / mL using the sample diluent. 80 μL of the adenovirus antigen standard solution of 6 concentrations was added to the test strips. Due to capillary action, the sample moved along the test strips to the conjugate pad and nitrocellulose membrane until it flowed to the absorbent pad. The results were observed for 10 minutes, and the color development was invalid after 15 minutes.

[0051] Color rendering effect Figure 2 As shown, the quality control lines of all test strips are clearly and obviously displayed, indicating that the test strips can normally play the role of indicating antigens. When the adenovirus antigen concentration is ≥0.2ng / mL, the test line is colored (due to problems such as shooting angle, contrast, pixels, etc. Figure 2 The visual effect of the detection line is not obvious. By comparing the framed part, it can be seen that the test strip detection line does exist when the antigen concentration is 0.2 ng / mL). Therefore, the minimum detection limit of the adenovirus detection test strip provided by the present invention for adenovirus antigen is 0.2 ng / mL. When the adenovirus antigen concentration is ≥ 0.2 ng / mL, the detection line is colored, indicating that the adenovirus detection result is positive; when the adenovirus antigen concentration is < 0.2 ng / mL, the detection line is not colored, indicating that the adenovirus detection result is negative.

[0052] Comparative Example 1: Effect of Adjustment of Labeling Conditions on Detection Performance

[0053] (1) Adjust pH

[0054] Adenovirus detection test strips were prepared according to the method provided in Example 2, except that the amount of potassium carbonate solution added was adjusted to 0.01 M in step 2 to 5 μL, 10 μL, 20 μL and 25 μL, respectively. Then, the adenovirus antigen standard solution was tested according to the method described in Example 3. The test results are shown in Table 1.

[0055] Table 1 Effect of potassium carbonate addition adjustment on detection performance

[0056] Potassium carbonate addition amount of 0.01M 5 μL 10 μL 20 μL 25 μL Minimum detectable limit 0.5 ng / mL 0.3 ng / mL 0.2 ng / mL 0.5 ng / mL

[0057] From the results in Table 1, it can be seen that adjusting the amount of potassium carbonate solution added will affect the detection performance of the adenovirus test strip, resulting in an increase in the minimum detection limit to varying degrees.

[0058] (2) Adjusting the marking reaction time

[0059] The adenovirus detection test strip was prepared according to the method provided in Example 2, except that in step 2, when using high-concentration colloidal gold to label the mouse anti-human adenovirus detection antibody, the labeling reaction time after adding the mouse anti-human adenovirus detection antibody was adjusted to 30 minutes, 60 minutes, and 120 minutes respectively. Then, the adenovirus antigen standard solution was detected according to the method described in Example 3, and the detection results are shown in Table 2.

[0060] Table 2 Influence of adjusted labeling reaction time on detection performance

[0061] Labeling reaction time 30 min 60 min 120 min Minimum detectable limit 1 ng / mL 0.5 ng / mL 0.2 ng / mL

[0062] As can be seen from the results in Table 2, adjusting the labeling reaction time will affect the detection performance of the adenovirus detection test strip, resulting in varying degrees of increase in the lowest detection limit.

[0063] (3) Adjust the composition of the blocking solution

[0064] The adenovirus detection test strip was prepared according to the method provided in Example 2, except that in step 2, the blocking solution was adjusted to 5 μL of 20 wt% bovine serum albumin. Then, the adenovirus antigen standard solution was detected according to the method described in Example 3, and the lowest detection limit was 0.5 ng / mL. When the adenovirus antigen concentration value ≥ 0.5 ng / mL, the detection result was positive; when < 0.5 ng / mL, the detection result was negative.

[0065] Comparative Example 2: Influence of adjusting the loading amounts of the detection antibody and the capture antibody on detection performance

[0066] (1) Adjust the loading amount of the detection antibody

[0067] The adenovirus detection test strip was prepared according to the method provided in Example 2, except that in step 4, 50 μL, 60 μL, 90 μL, 100 μL, and 110 μL of the colloidal gold-labeled complex solution were added to the precipitate after removing the supernatant when the mouse anti-human adenovirus detection antibody was labeled with colloidal gold. Then, the adenovirus antigen standard solution was detected according to the method described in Example 3, and the detection results are shown in Table 3.

[0068] Table 3 Influence of adjusted loading amount of the detection antibody on detection performance

[0069] Amount of gold-labeled complex solution added 50 μL 60 μL 90 μL 100 μL 110 μL Minimum detectable limit 0.2 ng / mL 0.2 ng / mL 0.4 ng / mL 0.5 ng / mL 0.8 ng / mL

[0070] As can be seen from the results in Table 3, increasing the loading amount of the detection antibody on the conjugate pad will affect the detection performance of the adenovirus detection test strip.

[0071] (2) Adjust the loading amount of the capture antibody

[0072] The adenovirus detection test strip was prepared according to the method provided in Example 2, except that in step 3, the concentrations of the mouse anti-human adenovirus capture antibody coating solution were adjusted to 0.2 mg / mL, 0.5 mg / mL, 1.8 mg / mL, 2.0 mg / mL, and 2.5 mg / mL, and then the adenovirus antigen standard solution was detected according to the method described in Example 3. The detection results are shown in Table 4.

[0073] Table 4 Influence of the adjustment of the capture antibody loading on the detection performance

[0074] Coating solution concentration 0.2 mg / mL 0.5 mg / mL 1.8 mg / mL 2.0 mg / mL 2.5 mg / mL Minimum detectable limit 1.0 ng / mL 0.5 ng / mL 0.2 ng / mL 0.2 ng / mL 0.5 ng / mL

[0075] As can be seen from the results in Table 4, increasing the loading of the capture antibody on the nitrocellulose membrane will affect the detection performance of the adenovirus detection test strip.

[0076] Obviously, the above examples are only for illustration and are not intended to limit the implementation. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementations here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. An adenovirus detection test strip based on high concentration colloidal gold, characterized in that: The adenovirus detection test strip is composed of a sample pad, a conjugation pad, a nitrocellulose membrane, a water-absorbing pad and a bottom plate, the conjugation pad is loaded with adenovirus detection antibodies labeled with high concentration colloidal gold, the nitrocellulose membrane is provided with a detection line, and the detection line is loaded with adenovirus capture antibodies; The particle size of the high concentration colloidal gold is 60 nm, and the optical density value is 100; The concentration of the adenovirus detection antibody is 50-80 μg / mL. After being mixed with high concentration colloidal gold for reaction for 1.2-1.5 hours, 90-100 μL of a labeling blocking solution is added for reaction for 20 minutes. The labeling blocking solution includes casein and boric acid, and the pH value of the labeling blocking solution is 8-9.

2. The adenovirus detection test strip according to claim 1, characterized in that: The steps for using high concentration colloidal gold to label adenovirus detection antibodies include: (1) Take high concentration colloidal gold, add adenovirus detection antibody, add labeling blocking solution after reaction, and centrifuge to obtain the precipitate; (2) adding a colloidal gold labeling solution to the precipitate, wherein the colloidal gold labeling solution comprises 35-60 mM buffer, 0.3-0.7 wt % casein, 10-30 wt % sucrose, 2-7 wt % trehalose and 0.02-0.06 wt % preservative.

3. The adenovirus detection test strip according to claim 2, characterized in that: In step (1), a potassium carbonate solution is used to adjust the pH value of the high concentration colloidal gold. The concentration of the potassium carbonate solution is 0.01 M and the amount added is 15-20 μL.

4. The adenovirus detection test strip according to claim 2, characterized in that: In step (2), the amount of the colloidal gold labeling solution added is 50-80 μg / mL.

5. The adenovirus detection test strip according to claim 1, characterized in that: The capture antibody is diluted with a coating diluent, which includes Tris, sucrose, bovine serum albumin and a preservative. After dilution, the concentration of the capture antibody is 0.7-2.0 mg / mL.

6. The adenovirus detection test strip according to claim 5, characterized in that: The diluted capture antibody was sprayed on the nitrocellulose membrane detection line at a volume of 1-2 μL / cm.

7. The adenovirus detection test strip according to claim 1, characterized in that: The adenovirus detection test strip also includes a sample diluent, which includes a buffer, casein, a surfactant and a preservative.

8. The method for preparing the adenovirus detection test strip according to claim 1, characterized in that: The steps include pretreating the sample pad and the conjugate pad, labeling the adenovirus detection antibody with high-concentration colloidal gold and spraying it on the conjugate pad, setting a detection line on the nitrocellulose membrane, spraying the adenovirus capture antibody on the detection line, and assembling the sample pad, the conjugate pad, the nitrocellulose membrane and the absorbent pad.

9. Use of the adenovirus detection test strip according to any one of claims 1 to 7 in detecting adenovirus antigens.

10. A method for detecting adenovirus antigens, characterized in that: The adenovirus detection colloidal gold test strip according to any one of claims 1 to 7 is used to detect the sample to be tested.

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