Volatile attractant for bactrocera dorsalis
A new type of orange fruit fly attractant was developed through the combination of ethyl acetate, butyl butyrate and dipentene, which solved the problem of identifying and passivation of commonly used attractants in the prior art, achieved efficient inducing effects, and supported the prevention and control of orange fruit fly.
Patent Information
- Application Number
- CN202510189651.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-20
- Publication Date
- 2025-06-24
AI Technical Summary
In the prior art, the identification and passivation of the commonly used attractant methyl eugenol by the orange fruit fly is caused by greatly reducing the trapping effect. It is urgent to develop a new orange fruit fly attractant.
Through research, it was found that after the combination of three compounds, ethyl acetate, butyl butyrate and dipentene, it has a strong inducing effect on orange fruit flies, providing a lure for orange fruit flies, including these compounds.
This attractant can effectively reduce the insect density of adults of orange fruit fly in the field, has excellent inducing effects, supports the prevention and control of orange fruit fly, and enriches the compound resources for biological control of fruit fly pests.
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Figure CN120188786A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of insect attractants, and more specifically, relates to a volatile attractant for oriental fruit flies. Background Art
[0002] Bactrocera dorsalis Hendel, Diptera, Tephritidae, Genus Bactrocera, is widely distributed in Guangdong, Guangxi, Yunnan and other places in my country. Common fruits such as citrus, mango and banana are the main hosts of Bactrocera dorsalis Hendel. The female insect uses an ovipositor to cut the peel of the fruit and lay eggs inside the fruit. After the eggs hatch, the larvae begin to feast on the flesh. When the larvae mature, they drill out of the fruit, dive into the soil to pupate, and finally emerge as adults, starting a new cycle. The fruit that has been bored by the larvae will first turn yellow. In severe cases, the fruit will fall off and rot directly, bringing a huge blow to the orchard.
[0003] At present, there are two main means of controlling the orange fruit fly in the field. One is to bag the fruit. Although this method can play a certain protective role, bagging is labor-intensive and has a high economic cost. The second is to use methyl eugenol for trapping. Methyl eugenol is a common commercial attractant on the market. It can attract the orange fruit fly and then kill it. However, due to the long-term and frequent use of methyl eugenol to attract the orange fruit fly, the orange fruit fly's recognition of methyl eugenol has been blunted, and the trapping effect has been greatly reduced. Therefore, it is urgent to develop a new type of orange fruit fly attractant. Summary of the invention
[0004] The present invention aims to develop an attractant that can effectively attract adult fruit flies. The present invention finds through research that three compounds, namely ethyl acetate, butyl butyrate and dipentene, have significant attracting effects on fruit flies. Based on this, an attractant for fruit flies is provided.
[0005] The purpose of the invention is to provide an attractant for oriental fruit flies and application thereof.
[0006] The above-mentioned purpose of the present invention is achieved through the following technical solutions:
[0007] The present invention has found that the combination of ethyl acetate, butyl butyrate and dipentene has a strong attractant effect on the oriental fruit fly, and therefore claims protection for the following scheme:
[0008] The invention provides an attractant for oriental fruit flies, which comprises ethyl acetate, butyl butyrate and dipentene.
[0009] Preferably, the mass ratio of ethyl acetate, butyl butyrate and dipentene is 1:(1-3):(1-3).
[0010] More preferably, the mass ratio of ethyl acetate, butyl butyrate and dipentene is 1:1:1.
[0011] Preferably, the concentration of ethyl acetate is 50 - 150 μg / mL, the concentration of butyl butyrate is 50 - 150 μg / mL, and the concentration of dipentene is 50 - 150 μg / mL.
[0012] More preferably, the concentration of ethyl acetate is 50 - 100 μg / mL, the concentration of butyl butyrate is 100 - 150 μg / mL, and the concentration of dipentene is 100 - 150 μg / mL.
[0013] Even more preferably, the concentration of ethyl acetate is 100 μg / mL, the concentration of butyl butyrate is 100 μg / mL, and the concentration of dipentene is 100 μg / mL.
[0014] As an alternative embodiment, the solvent of ethyl acetate, butyl butyrate and dipentene is liquid paraffin.
[0015] Use of the above attractant in attracting Bactrocera dorsalis.
[0016] Use of the above attractant in preparing a product for attracting Bactrocera dorsalis.
[0017] Use of the above attractant in controlling Bactrocera dorsalis.
[0018] Use of the above attractant in preparing a product for controlling Bactrocera dorsalis.
[0019] Use of the above attractant in controlling plant diseases caused by Bactrocera dorsalis.
[0020] Use of the combination of the above attractant and insecticide in preparing a product for trapping and killing Bactrocera dorsalis.
[0021] The present invention has the following beneficial effects:
[0022] The attractant of the present invention can effectively reduce the population density of adult Bactrocera dorsalis in the field. The attractant developed by the present invention is a novel attractant for Bactrocera dorsalis. The raw materials of the attractant are low in cost, wide in acquisition channels, simple in formula, easy in production process, and have excellent attracting effects on Bactrocera dorsalis. The attractant of the present invention can provide strong support for the control of Bactrocera dorsalis, enrich the compound resources for biological control of fruit fly pests, and promote the development of integrated pest control strategies towards high efficiency, economy, and greenness, and has high application value. Description of the Drawings
[0023] Figure 1 To determine the selection rate of Bactrocera dorsalis under different experimental group mixtures in the trap experiment.
[0024] Figure 2The trap experiment was conducted to determine the selection rate of Bactrocera dorsalis (Hendel) for mixtures of ethyl acetate, butyl butyrate, and dipentene with different ratios. Specific Embodiments
[0025] The present invention will be further described below in conjunction with the accompanying drawings of the specification and specific embodiments. However, the embodiments do not limit the present invention in any form. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in the technical field.
[0026] Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.
[0027] In the following examples, the adult Bactrocera dorsalis (Hendel) used was captured in the Carambola Park in Guangzhou and bred in the laboratory for more than 30 generations under the condition of a photoperiod of light period (L): dark period (D) = 14:10. The larval diet of Bactrocera dorsalis (Hendel) was prepared from corn flour, sucrose, yeast powder, cellulose (paper towel), purified water, hydrochloric acid, and sodium benzoate according to a mass ratio of 250:25:25:25:200:2:1. The adult diet of Bactrocera dorsalis (Hendel) was prepared from sucrose and yeast powder according to a mass ratio of 3:1.
[0028] The trap experiment in the examples refers to the experimental method in the reference "GU F, AI S, CHEN Y, et al. Mutualism promotes insect fitness by fungal nutrient compensation and facilitates fungus propagation by mediating insect oviposition preference[J]. ISME J, 2022, 16(7):1831 - 1842."
[0029] Ethyl acetate, butyl butyrate, dipentene, and liquid paraffin were purchased from Shanghai Macklin Biochemical Co., Ltd.; the product numbers are E809174, E807063, E802160, and E81570, respectively;
[0030] Ethyl acetate, with a CAS number of 414 - 78 - 6, has the following structural formula:
[0031] Butyl butyrate, with a CAS number of 109 - 21 - 7, has the following structural formula:
[0032] Dipentene (DL - Limonene), with a CAS number of 138 - 86 - 3, has the following structural formula:
[0033] d-Limonene, with a CAS number of 7705-14-8, has the following structural formula:
[0034] Example 1
[0035] (I) Experimental method
[0036] For the indoor selectivity determination, a trap experiment was used for testing. The experiment was carried out in an insect rearing cage with dimensions of 30 cm × 30 cm × 30 cm.
[0037] Experimental group: Using liquid paraffin as a solvent, mixture solutions of different experimental groups were prepared according to the formula in Table 1;
[0038] Negative control group: Liquid paraffin with the same volume as the mixture solution of the experimental group.
[0039] Table 1 Formulas of mixture solutions for different experimental groups
[0040]
[0041] Specific experimental method of the trap experiment:
[0042] 200 μL of the mixture solution of the experimental group and the negative control group (liquid paraffin) were respectively dropped onto filter papers. The filter papers were placed in trap bottles. The trap bottles of the experimental group and the negative control group were placed diagonally. After 24 h, the number of insects in the trap bottles of the experimental group and the control group was observed and counted. Each experimental group was repeated 3 times, with 50 insects in each group. After each test, the insect rearing cage was cleaned, and the trap bottles were cleaned with absolute ethanol and water and then placed in an oven to dry, so as to avoid the influence of the smell during the test on the next experiment. The test was carried out under the condition of 28 - 30 °C. The selection rate of different experimental groups for adult Bactrocera dorsalis was calculated using the following formula.
[0043]
[0044] (II) Experimental results
[0045] The results of the trap experiment for determining the selection rate of Bactrocera dorsalis under the conditions of mixture solutions of different experimental groups are as Figure 1 shown. The results show that: After 24 h of attraction, Bactrocera dorsalis had a certain selection preference for experimental groups 1 - 6 and experimental group 8, but the selection rate was relatively low, only about 40%, and the attraction effect on Bactrocera dorsalis was not obvious. However, Bactrocera dorsalis had a more obvious selection preference for experimental group 7, and the average selection rate could reach 85%, indicating that adult Bactrocera dorsalis had an obvious selection preference for the mixture of ethyl acetate, butyl butyrate, and dipentene.
[0046] Example 2
[0047] (I) Experimental method
[0048] For the selective determination indoors, trap experiments were conducted and tested in an insect rearing cage of 30 cm × 30 cm × 30 cm.
[0049] Experimental groups: Prepare mixed solution of different experimental groups according to the formula in Table 2 (using liquid paraffin as the solvent);
[0050] Negative control group: Liquid paraffin with the same volume as the mixed solution of the experimental group.
[0051] For the specific experimental method of the trap experiment, refer to Example 1.
[0052] Table 2 Formula of the mixed solution
[0053]
[0054] (II) Experimental results
[0055] The selection rates of Bactrocera dorsalis for mixtures of ethyl acetate, butyl butyrate, and dipentene with different ratios are as Figure 2 shown. The results show that: the selection rates of Bactrocera dorsalis for experimental groups 10, 11, and 12 have no significant difference from that of experimental group 7. The selection rate of experimental group 9 has a significant difference compared with that of experimental group 7, but the selection rate is still 73%, which is much higher than that of experimental groups 1-6 and experimental group 8.
[0056] In summary, an adult attractant for Bactrocera dorsalis provided by the present invention, the attractant includes ethyl acetate, butyl butyrate, and dipentene, has an excellent effect of attracting Bactrocera dorsalis, and clarifies that the mixture of these three substances, ethyl acetate, butyl butyrate, and dipentene, has a high attracting activity for adults of Bactrocera dorsalis, and the attracting rate is more than 85%. Therefore, the mixture of ethyl acetate, butyl butyrate, and dipentene can be used as an attractant for adults of Bactrocera dorsalis or as an attracting synergist, and has potential practical application prospects in the research and development of integrated pest control.
[0057] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.
Claims
1. An attractant for Bactrocera dorsalis, characterized in that: These include ethyl acetate, butyl butyrate and dipentene.
2. The attractant according to claim 1, characterized in that: The mass ratio of ethyl acetate, butyl butyrate and dipentene is 1:(1-3):(1-3).
3. The attractant according to claim 1, characterized in that: The concentration of ethyl acetate is 50-150 μg / mL, the concentration of butyl butyrate is 50-150 μg / mL, and the concentration of dipentene is 50-150 μg / mL.
4. The sex attractant according to claim 3, characterized in that: The solvent for ethyl acetate, butyl butyrate and dipentene is liquid paraffin.
5. Use of the attractant according to any one of claims 1 to 4 in attracting Bactrocera dorsalis.
6. Use of the attractant according to any one of claims 1 to 4 in preparing a product for attracting oriental fruit fly.
7. Use of the attractant according to any one of claims 1 to 4 in controlling the oriental fruit fly.
8. Use of the attractant according to any one of claims 1 to 4 in the preparation of a product for controlling oriental fruit fly.
9. Use of the attractant according to any one of claims 1 to 4 in preventing and controlling plant diseases caused by Bactrocera dorsalis.
10. Use of the attractant and insecticide combination according to any one of claims 1 to 4 in preparing a product for luring and killing oriental fruit flies.