Traditional Chinese medicinal material active ingredient extraction method based on ultra-low temperature crushing and deep eutectic solvent synergy, tablet candy capable of dispelling effects of alcohol and protecting liver and preparation method of tablet candy

Abstract

The invention discloses a method for extracting active ingredients of traditional Chinese medicinal materials based on ultralow-temperature crushing and deep eutectic solvent synergy, a tableted candy for dispelling effects of alcohol and protecting liver and a preparation method of the tableted candy. The ultralow-temperature crushing and deep eutectic solvent synergy extraction technology is combined, and the active ingredients of the traditional Chinese medicinal materials with homology of medicine and food are extracted at low temperature; the method comprises the following steps: firstly, quickly freezing a raw material by using liquid nitrogen and mechanically crushing the raw material to improve the cell wall breaking rate, then stirring and mixing a deep eutectic solvent based on choline chloride and glycerol with water to reduce the viscosity of the solvent for extraction, and carrying out ultrasonic extraction, filtration, macroporous adsorption resin recovery and other steps to obtain a high-purity extract. According to the preparation method, the extraction rate of active substances in the traditional Chinese medicinal materials is increased, and extracts of multiple medicinal and edible Chinese herbal medicines such as lucid ganoderma, radix puerariae, hawthorn and olive are compounded into the tablet candy, so that liver injury caused by alcohol is effectively improved, and side effects caused by long-term administration of medicines can be effectively avoided.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods, relates to hangover and liver-protecting candies, and specifically relates to a method for extracting active ingredients of Chinese herbal medicines based on the synergism of ultra-low temperature crushing and deep eutectic solvents, a tabletting candy for hangover and liver protection, and a preparation method thereof. Background Art

[0002] Alcoholic liver injury is a liver injury problem worldwide. Long-term and excessive alcohol consumption can lead to abnormal liver function, promote the occurrence of alcoholic fatty liver, and in severe cases, develop into liver fibrosis, cirrhosis, liver cancer and other diseases. Therefore, it is of great significance to develop a tabletting candy with hangover and liver protection functions. Clinically used drugs for treating alcoholic liver injury include polyene phosphatidylcholine capsules, silybin capsules, etc., but their adverse reactions are unknown and they are not suitable for long-term use. However, Chinese herbal medicines with both medicinal and edible properties overcome this problem. They not only have good activity, but also have good safety and can be taken for a long time. Compared with clinical drugs such as polyene phosphatidylcholine capsules and silybin capsules, the application value of extracts from Chinese herbal medicines with both medicinal and edible properties is broader.

[0003] Ganoderma lucidum is a traditional Chinese herbal medicine with both medicinal and edible properties. It has strong bioactive ingredients such as polysaccharides and triterpenoids, and is widely used in liver protection, antioxidant, anti-liver injury, etc. Currently, the main method for extracting active ingredients from Chinese herbal medicines with both medicinal and edible properties is hot water extraction. However, the high-temperature extraction method will cause the loss of heat-labile active ingredients. Summary of the Invention

[0004] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide a method for extracting active ingredients of Chinese herbal medicines based on the synergism of ultra-low temperature crushing and deep eutectic solvents, a tabletting candy for hangover and liver protection, and a preparation method thereof, to improve the extraction rate of active substances in Chinese herbal medicines, and the prepared tabletting candy can effectively improve liver damage caused by alcohol and avoid the side effects brought by long-term drug use.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A method for extracting active ingredients of Chinese herbal medicines based on the synergism of ultra-low temperature crushing and deep eutectic solvents, comprising the following steps:

[0007] Step 1: Rapidly freeze the Chinese herbal medicine raw materials with liquid nitrogen, and then perform mechanical crushing to obtain Chinese herbal medicine powder;

[0008] Step 2: Mix choline chloride and glycerol in a volume ratio of 1:(1-2), add water with a volume ratio of 15-25%, and stir evenly to form a homogeneous and stable deep eutectic solvent;

[0009] Step 3: Add the Chinese medicinal material powder prepared in Step 1 to the deep eutectic solvent prepared in Step 2 according to the mass-to-volume ratio of 1 g:(20 - 40) mL, and perform ultrasonic extraction for 30 - 40 min. After the extraction is completed, filter to remove the filter residue to obtain the extract.

[0010] Step 4: Adsorb the extract obtained in Step 3 with ME-2 macroporous adsorption resin, then perform elution, rotary evaporation, and freeze-drying to obtain the active extract.

[0011] Preferably, the operation method of the rapid freezing in Step 1 is: Add liquid nitrogen to the medicinal material raw materials according to the volume ratio of liquid nitrogen to medicinal material raw materials of (2 - 3):1 to rapidly freeze the medicinal materials.

[0012] Preferably, the stirring in Step 2 is carried out using a magnetic stirrer at 80 - 90 °C for 6 - 8 h.

[0013] Preferably, the ultrasonic frequency of the ultrasonic extraction in Step 3 is 350 - 450 W.

[0014] Preferably, the elution in Step 4 is to first wash and remove the deep eutectic solvent with distilled water, and then sequentially elute with 30%, 60%, 90%, and 100% ethanol and combine the eluents.

[0015] The present invention also protects a tablet candy for relieving hangover and protecting the liver, and the raw materials include, by weight: 5 - 15 parts of ganoderma lucidum extract, 5 - 10 parts of pueraria lobata extract, 4 - 8 parts of hovenia dulcis extract, 5 - 10 parts of hawthorn extract, 4 - 5 parts of olive extract, 3 - 9 parts of xylitol, 1 - 2 parts of citric acid, 5 - 10 parts of microcrystalline cellulose, 15 - 30 parts of maltodextrin, and 0.5 - 1 part of magnesium stearate;

[0016] The ganoderma lucidum extract, pueraria lobata extract, hovenia dulcis extract, hawthorn extract, and olive extract are extracted by the method described above.

[0017] The preparation method of the tablet candy for relieving hangover and protecting the liver as described above includes the following steps:

[0018] Step 1: Take the raw materials according to the ratio and mix them.

[0019] Step 2: Place the mixed materials in a stainless-steel tray, spray 70% - 80% ethanol until moistened, and then granulate.

[0020] Step 3: Pass the granules prepared in Step 1 through a 20-mesh sieve, dry them, and then perform sieving again.

[0021] Step 4: Add magnesium stearate to the granules obtained in Step 3, mix them evenly, and use a single-punch tablet press to press tablets to obtain the tablet candy for relieving hangover and protecting the liver.

[0022] Preferably, the mixing in step one is carried out using a V-type mixer with a rotation speed of 10-15 rpm for 10-20 minutes.

[0023] Preferably, the drying in step three is carried out by placing it in a constant temperature drying oven at 50-60 °C for 6-8 hours.

[0024] Preferably, the granulation method in step three is to pass through an 80-100 mesh sieve.

[0025] Compared with the prior art, the present invention has the following technical effects:

[0026] The present invention extracts active compounds at low temperature by adopting the method of ultra-low temperature crushing and deep eutectic solvent synergistic extraction to improve the cell wall breaking rate, significantly improving the extraction rate and functional characteristics of Chinese medicinal materials and food homologous Chinese medicinal materials, solving the problems of high energy consumption, low extraction rate and insufficient functionality in traditional methods, promoting the application of active extracts in the field of food health, and the method is simple to operate, green and environmentally friendly, and the extraction solution can be reused with low cost;

[0027] The present invention combines the active extracts of Ganoderma lucidum, Pueraria lobata, Hovenia dulcis, Canarium album and Crataegus pinnatifida together by compounding, combining traditional Chinese medicine theory and experimental results, and the several components synergistically enhance the efficacy of Chinese medicinal materials and food homologous Chinese herbal medicines, and prepares Ganoderma lucidum tablets with the efficacy of relieving alcohol and protecting the liver. The tablets can effectively improve the liver damage caused by alcohol, the formula is simple, all are extracts of Chinese medicinal materials and food homologous Chinese medicinal materials, which can avoid the side effects caused by long-term taking of drugs, have a wide application scenario, high safety and good activity, and are suitable for long-term taking. Description of the Drawings

[0028] Figure 1 It is a comparison chart of the influence of the hot water extraction method of Comparative Example 1 and the ultra-low temperature crushing synergistic deep eutectic solvent extraction method of Example 1 on the activity of Ganoderma lucidum extract;

[0029] Figure 2 It is the network pharmacology analysis of the tablets for relieving alcohol and protecting the liver prepared in Example 6;

[0030] Figure 3 It is a comparison chart of the effect of the tablets for relieving alcohol and protecting the liver prepared in Example 6 on mice's relieving alcohol and protecting the liver. Detailed Description of the Invention

[0031] The following further elaborates on the specific content of the present invention in conjunction with examples.

[0032] In the Chinese medicinal material extract of the present invention, the Ganoderma lucidum extract is extracted from the fruiting body, the Pueraria lobata is extracted from the root, and the Hovenia dulcis, Crataegus pinnatifida and Canarium album are all extracted from the fruits.

[0033] Example 1

[0034] This embodiment provides a method for extracting active ingredients from traditional Chinese medicine based on the synergism of ultra-low temperature crushing and deep eutectic solvents, including the following steps:

[0035] Step 1: Add liquid nitrogen to Ganoderma lucidum at a volume ratio of liquid nitrogen to raw medicinal materials of 2:1 to quickly freeze the medicinal materials. After 10 minutes, scoop them out with a ladle, then use a pulverizer to crush them. Run for 10 seconds and stop for 10 seconds, with a total of 5 cycles. Then screen them with a 100-mesh sieve to obtain traditional Chinese medicine powder.

[0036] Step 2: Mix choline chloride and glycerol at a volume ratio of 1:2, add water with a volume fraction of 15%, put the mixture into a magnetic stirrer, and stir at 80 °C and a rotation speed of 50 rpm for 6 hours until it becomes uniform to form a homogeneous and stable deep eutectic solvent.

[0037] Step 3: Add the traditional Chinese medicine powder prepared in Step 1 to the deep eutectic solvent prepared in Step 2 at a mass-volume ratio of 1 g:40 mL, shake for 1 minute to make them mix evenly, extract in an ultrasonic wave of 400 W for 30 minutes. After the extraction is completed, filter to remove the filter residue to obtain the extract.

[0038] Step 4: Soak the ME-2 macroporous adsorption resin in absolute ethanol and fill it into a chromatographic column with a size of φ50×400 mm. First, rinse it with 10 times the column volume of absolute ethanol (25 mL / min), and then rinse it with distilled water until there is no ethanol smell (25 mL / min). Use the ME-2 macroporous adsorption resin to recover flavonoids from the obtained extract. Add the extract to the ME-2 macroporous adsorption resin for adsorption, and elute it with distilled water (1 mL / min) for 10 times the column volume to remove the deep eutectic solvent. Then elute it successively with 30%, 60%, 90%, and 100% ethanol for 10 times the column volume (1 mL / min). Combine the eluates, and rotary evaporate and concentrate them at 40 °C until there is no ethanol, and then freeze-dry them with a freeze dryer to obtain the active extract.

[0039] Example 2

[0040] This embodiment provides a method for extracting active ingredients from traditional Chinese medicine based on the synergism of ultra-low temperature crushing and deep eutectic solvents, including the following steps:

[0041] Step 1: Add liquid nitrogen to hawthorn raw materials at a volume ratio of liquid nitrogen to raw medicinal materials of 3:1 to quickly freeze the medicinal materials. After 10 minutes, scoop them out with a ladle, then use a pulverizer to crush them. Run for 10 seconds and stop for 10 seconds, with a total of 10 cycles. Then screen them with a 100-mesh sieve to obtain traditional Chinese medicine powder.

[0042] Step 2: Mix choline chloride and glycerol in a volume ratio of 1:1, add water with a volume ratio of 25%, put the mixture into a magnetic stirrer, and stir at 80°C and a rotation speed of 60 rpm for 8 h until it is uniform to form a homogeneous and stable deep eutectic solvent;

[0043] Step 3: Add the traditional Chinese medicine powder prepared in Step 1 to the deep eutectic solvent prepared in Step 2 according to a mass-volume ratio of 1 g:30 mL, shake for 1 min to make it evenly mixed, extract in an ultrasonic wave of 400 W for 40 min, and after the extraction is completed, filter to remove the filter residue to obtain the extract;

[0044] Step 4: Soak the ME-2 macroporous adsorption resin with absolute ethanol, fill it into a chromatographic column with a size of φ50×400 mm, first rinse it with 10 times the column volume of absolute ethanol (25 mL / min), and then rinse it with distilled water until there is no ethanol smell (25 mL / min). Use the ME-2 macroporous adsorption resin to recover flavonoids from the obtained extract. Add the extract to the ME-2 macroporous adsorption resin for adsorption, and elute it with distilled water (1 mL / min) for 10 times the column volume to remove the deep eutectic solvent. Then elute it with 30%, 60%, 90%, and 100% ethanol for 10 times the column volume (1 mL / min) in sequence, combine the eluates, and rotary evaporate and concentrate them at 50°C until there is no ethanol, and then freeze-dry them with a freeze dryer to obtain the active extract.

[0045] Example 3

[0046] This example provides a method for extracting active ingredients from traditional Chinese medicine based on the synergism of ultra-low temperature crushing and deep eutectic solvents, including the following steps:

[0047] Step 1: Add liquid nitrogen to the pueraria lobata raw material to quickly freeze the medicinal materials according to a volume ratio of liquid nitrogen to medicinal material raw material of 2.5:1. After 10 min, scoop it out with a ladle, then use a pulverizer to crush it, run for 10 s and stop for 10 s, with a total of 8 cycles, and then screen it with a 100-mesh sieve to obtain the traditional Chinese medicine powder;

[0048] Step 2: Mix choline chloride and glycerol in a volume ratio of 1:1.5, add water with a volume ratio of 20%, put the mixture into a magnetic stirrer, and stir at 80°C and a rotation speed of 55 rpm for 7 h until it is uniform to form a homogeneous and stable deep eutectic solvent;

[0049] Step 3: Add the traditional Chinese medicine powder prepared in Step 1 to the deep eutectic solvent prepared in Step 2 according to a mass-volume ratio of 1 g:35 mL, shake for 1 min to make it evenly mixed, extract in an ultrasonic wave of 400 W for 35 min, and after the extraction is completed, filter to remove the filter residue to obtain the extract;

[0050] Step 4: Soak the ME-2 macroporous adsorption resin in absolute ethanol, fill it into a chromatographic column with a size of φ50×400 mm. First, rinse it with 10 column volumes of absolute ethanol (25 mL / min), and then rinse it with distilled water until there is no ethanol smell (25 mL / min). Use the ME-2 macroporous adsorption resin to recover flavonoids from the obtained extract. Add the extract to the ME-2 macroporous adsorption resin for adsorption, and elute it with distilled water (1 mL / min) for 10 column volumes to remove the deep eutectic solvent. Then, elute it successively with 30%, 60%, 90%, and 100% ethanol for 10 column volumes (1 mL / min). Combine the eluates, and rotary evaporate and concentrate them at 45°C until there is no ethanol, and then freeze-dry them with a freeze dryer to obtain the active extract.

[0051] Example 4

[0052] This example provides a method for extracting active ingredients from traditional Chinese medicine based on the synergism of ultra-low temperature crushing and deep eutectic solvents, including the following steps:

[0053] Step 1: Add liquid nitrogen to the olive raw materials according to the volume ratio of liquid nitrogen to medicinal raw materials of 2:1 to quickly freeze the medicinal materials. After 10 minutes, scoop them out with a ladle, and then use a pulverizer to crush them. Run for 10 seconds and stop for 10 seconds, with a total of 10 cycles. Then, screen them with a 100-mesh sieve to obtain the traditional Chinese medicine powder.

[0054] Step 2: Mix choline chloride and glycerol according to the volume ratio of 1:2, add water with a volume fraction of 20%, put the mixture into a magnetic stirrer, and stir it at 80°C and a rotation speed of 60 rpm for 6 hours until it is uniform to form a uniform and stable deep eutectic solvent.

[0055] Step 3: Add the traditional Chinese medicine powder prepared in Step 1 to the deep eutectic solvent prepared in Step 2 according to the mass-volume ratio of 1 g:20 mL, shake it for 1 minute to make it mix evenly, extract it in an ultrasonic wave with a power of 400 W for 40 minutes. After the extraction is completed, filter out the residue by suction filtration to obtain the extract.

[0056] Step 4: Soak the ME-2 macroporous adsorption resin in absolute ethanol, fill it into a chromatographic column with a size of φ50×400 mm. First, rinse it with 10 column volumes of absolute ethanol (25 mL / min), and then rinse it with distilled water until there is no ethanol smell (25 mL / min). Use the ME-2 macroporous adsorption resin to recover flavonoids from the obtained extract. Add the extract to the ME-2 macroporous adsorption resin for adsorption, and elute it with distilled water (1 mL / min) for 10 column volumes to remove the deep eutectic solvent. Then, elute it successively with 30%, 60%, 90%, and 100% ethanol for 10 column volumes (1 mL / min). Combine the eluates, and rotary evaporate and concentrate them at 40°C until there is no ethanol, and then freeze-dry them with a freeze dryer to obtain the active extract.

[0057] Example 5

[0058] This example provides a method for extracting active ingredients from Chinese herbal medicines by synergistically combining cryogenic crushing and deep eutectic solvents, including the following steps:

[0059] Step 1: Add liquid nitrogen to the Hovenia dulcis Thunb. raw material according to the volume ratio of liquid nitrogen to medicinal raw material of 2:1 to quickly freeze the medicinal material. After 10 minutes, scoop it out with a strainer, then use a pulverizer to crush it. Run for 10 seconds and stop for 10 seconds, for a total of 10 cycles. Then screen it with a 100-mesh sieve to obtain Chinese herbal medicine powder;

[0060] Step 2: Mix choline chloride and glycerol according to the volume ratio of 1:2, add water with a volume ratio of 30%, put the mixture into a magnetic stirrer, and stir at 80 °C and a rotation speed of 60 rpm for 7 hours until it is uniform to form a homogeneous and stable deep eutectic solvent;

[0061] Step 3: Add the Chinese herbal medicine powder prepared in Step 1 to the deep eutectic solvent prepared in Step 2 according to the mass-volume ratio of 1 g:35 mL, shake for 1 minute to make it evenly mixed, extract in an ultrasonic wave of 400 W for 40 minutes. After the extraction is completed, filter to remove the filter residue to obtain the extract;

[0062] Step 4: Soak the ME-2 macroporous adsorption resin in absolute ethanol, fill it into a chromatographic column with a diameter of φ50×400 mm. First, rinse it with 10 times the column volume of absolute ethanol (25 mL / min), and then rinse it with distilled water until there is no ethanol smell (25 mL / min). Use the ME-2 macroporous adsorption resin to recover flavonoids from the obtained extract. Add the extract to the ME-2 macroporous adsorption resin for adsorption, and elute it with distilled water (1 mL / min) for 10 times the column volume to remove the deep eutectic solvent. Then elute it with 30%, 60%, 90%, and 100% ethanol for 10 times the column volume (1 mL / min) in sequence. Combine the eluates, and rotate and evaporate and concentrate them at 45 °C until there is no ethanol, and then freeze-dry them with a freeze-dryer to obtain the active extract.

[0063] Example 6

[0064] This example provides a chewable tablet candy for relieving hangover and protecting the liver. The raw materials include, by weight: 5 parts of Ganoderma lucidum extract, 5 parts of Pueraria lobata extract, 4 parts of Hovenia dulcis Thunb. extract, 5 parts of Crataegus pinnatifida extract, 4 parts of Canarium album extract, 3 parts of xylitol, 1 part of citric acid, 5 parts of microcrystalline cellulose, 15 parts of maltodextrin, and 0.5 part of magnesium stearate;

[0065] The preparation method of the chewable tablet candy for relieving hangover and protecting the liver includes the following steps:

[0066] Step 1: Weigh the raw materials according to the ratio and mix them in a V-type mixer with a rotation speed of 10 rpm for 10 minutes;

[0067] Step 2: Place the mixed materials in a stainless-steel tray, spray 70% ethanol until moistened, and then granulate.

[0068] Step 3: Pass the granules prepared in Step 1 through a 20-mesh sieve, place them in a constant-temperature drying oven, dry at 50°C for 6 - 8 hours, and then pass them through an 80-mesh sieve for sizing.

[0069] Step 4: Add magnesium stearate to the granules obtained in Step 3, mix evenly, use a single-punch tablet press for tableting, and adjust the tablet weight to 0.5 g to obtain the anti-alcoholic and liver-protecting tablet candies.

[0070] Example 7

[0071] This example provides an anti-alcoholic and liver-protecting tablet candy. The raw materials, by weight, include: 15 parts of ganoderma lucidum extract, 10 parts of pueraria lobata extract, 8 parts of hovenia dulcis extract, 10 parts of hawthorn extract, 5 parts of olive extract, 9 parts of xylitol, 2 parts of citric acid, 10 parts of microcrystalline cellulose, 30 parts of maltodextrin, and 1 part of magnesium stearate.

[0072] The preparation method of the anti-alcoholic and liver-protecting tablet candy includes the following steps:

[0073] Step 1: Weigh the raw materials according to the ratio and mix them in a V-type mixer with a rotation speed of 15 rpm for 20 minutes.

[0074] Step 2: Place the mixed materials in a stainless-steel tray, spray 80% ethanol until moistened, and then granulate.

[0075] Step 3: Pass the granules prepared in Step 1 through a 20-mesh sieve, place them in a constant-temperature drying oven, dry at 60°C for 8 hours, and then pass them through a 100-mesh sieve for sizing.

[0076] Step 4: Add magnesium stearate to the granules obtained in Step 3, mix evenly, use a single-punch tablet press for tableting, and adjust the tablet weight to 0.5 g to obtain the anti-alcoholic and liver-protecting tablet candies.

[0077] Example 8

[0078] This example provides an anti-alcoholic and liver-protecting tablet candy. The raw materials, by weight, include: 10 parts of ganoderma lucidum extract, 8 parts of pueraria lobata extract, 6 parts of hovenia dulcis extract, 8 parts of hawthorn extract, 4.5 parts of olive extract, 5 parts of xylitol, 1.5 parts of citric acid, 8 parts of microcrystalline cellulose, 20 parts of maltodextrin, and 0.6 part of magnesium stearate.

[0079] The preparation method of the anti-alcoholic and liver-protecting tablet candy includes the following steps:

[0080] Step 1: Weigh the raw materials according to the ratio and mix them in a V-type mixer with a rotation speed of 12 rpm for 15 minutes.

[0081] Step 2: Place the mixed materials in a stainless-steel tray, spray 75% ethanol until moistened, and then granulate.

[0082] Step 3: Pass the granules prepared in Step 1 through a 20-mesh sieve, put them into a constant-temperature drying oven and dry at 55 °C for 7 h, and then pass them through a 90-mesh sieve for sizing.

[0083] Step 4: Add magnesium stearate to the granules obtained in Step 3, mix evenly, use a single-punch tablet press for tableting, and adjust the tablet weight to 0.5 g to obtain the anti-alcoholic and liver-protecting tablet candies.

[0084] Comparative Example 1

[0085] A method for hot water extraction of Ganoderma lucidum active ingredients, comprising the following steps:

[0086] Step 1: Take Ganoderma lucidum medicinal materials with the same mass as in Example 1, then use a pulverizer to crush them, run for 10 s and stop for 10 s, with a total of 5 cycles, and then screen through a 100-mesh sieve to obtain Chinese medicinal material powder.

[0087] Step 2: Add the Chinese medicinal material powder prepared in Step 1 to water according to the mass-to-volume ratio of 1 g:40 mL, shake for 1 min to mix evenly, heat and stir at 80 °C for 30 min, cool to room temperature after extraction, and filter to remove the filter residue to obtain the extract.

[0088] Step 3: Soak the ME-2 macroporous adsorption resin in anhydrous ethanol, fill it into a chromatographic column with a diameter of φ50×400 mm, first rinse with 10 column volumes of anhydrous ethanol (25 mL / min), and then rinse with distilled water until there is no ethanol smell (25 mL / min). Use the ME-2 macroporous adsorption resin to recover flavonoids from the obtained extract, add the extract to the ME-2 macroporous adsorption resin for adsorption, and elute with distilled water (1 mL / min) for 10 column volumes to remove the deep eutectic solvent. Then elute with 30%, 60%, 90%, and 100% ethanol for 10 column volumes (1 mL / min) in sequence, combine the eluates, and rotary evaporate and concentrate at 40 °C until there is no ethanol, and then freeze-dry with a freeze-dryer to obtain the active extract.

[0089] Perform activity detection on the Ganoderma lucidum active extracts of Example 1 and Comparative Example 1, and the results are as Figure 1 shown. The activity of the Ganoderma lucidum extract shows a dose-dependent relationship. The DPPH scavenging rate, ABTS + scavenging rate, and Fe 3+ reducing power of the Ganoderma lucidum extract obtained by hot water extraction (3.2 mg / mL) are 63.73%, 52.87%, and 44.17% respectively. However, for the Ganoderma lucidum extract obtained by deep eutectic solvent extraction, when its concentration is 3.2 mg / mL, its DPPH scavenging rate, ABTS +Clearance rate and Fe 3+ The reducing powers were increased to 75.93%, 69.03% and 65.37%, respectively. Therefore, the antioxidant activity of Ganoderma lucidum extract extracted by deep eutectic solvent was significantly higher than that obtained by hot water extraction.

[0090] Figure 2 For the network pharmacology analysis of the anti-hangover and liver-protecting tablet candy prepared in Example 6, as Figure 2 shown, by predicting the action targets of the compound and then comparing them with the targets of fatty liver, it was found that there were XX overlapping targets between the two. Then, the overlapping targets were enriched in the KEGG database for pathways, and it was found that the first pathway was fatty liver. Therefore, theoretically, this tablet candy has a certain improvement effect on fatty liver.

[0091] Figure 3 For the anti-hangover and liver-protecting effects of the anti-hangover and liver-protecting tablet candy prepared in Example 6 on mice; as Figure 3 can be seen, after using the Ganoderma lucidum tablet candy, the drunken time of the mice was prolonged. The drunken times of the low-dose and high-dose groups were prolonged by 1.64 times and 2.02 times, respectively, and the sobering-up times were shortened by 37.26% and 61.28%, respectively. And through the analysis of data such as ALT, AST, MDA, SOD in liver tissues, it was found that the anti-hangover and liver-protecting tablet candy of the present invention improved the oxidative stress of the liver and reduced the liver damage.

[0092] It should be noted that the above-described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art without making creative efforts based on the embodiments of the present invention fall within the scope of protection of the present invention.

Claims

1. A method for extracting active ingredients from traditional Chinese medicine based on ultra-low temperature crushing and deep eutectic solvent synergy, characterized in that: The following steps are involved: Step 1: Use liquid nitrogen to quickly freeze the Chinese medicinal material raw materials, and then mechanically crush them to obtain Chinese medicinal material powder; Step 2, choline chloride and glycerol are mixed in a volume ratio of 1:(1-2), and water is added in a volume ratio of 15-25%, and stirred evenly to form a uniform and stable deep eutectic solvent; Step 3: Add the Chinese medicinal material powder prepared in step 1 to the deep eutectic solvent prepared in step 2 at a mass volume ratio of 1 g: (20-40) mL, perform ultrasonic extraction for 30-40 min, and after the extraction is completed, remove the residue by suction filtration to obtain an extract; Step 4: adsorb the extract obtained in step 3 with ME-2 macroporous adsorption resin, and then elute, rotary evaporate and freeze-dry to obtain an active extract.

2. The method for extracting active ingredients from traditional Chinese medicines based on ultra-low temperature crushing and deep eutectic solvent synergy according to claim 1, characterized in that: The operation method of rapid freezing described in step 1 is: adding liquid nitrogen to the medicinal material raw material at a volume ratio of (2-3):1 to rapidly freeze the medicinal material.

3. The method for extracting active ingredients from traditional Chinese medicines based on ultra-low temperature crushing and deep eutectic solvent synergy according to claim 1, characterized in that: The stirring in step 2 is carried out by using a magnetic stirrer at 80-90° C. for 6-8 hours.

4. The method for extracting active ingredients from traditional Chinese medicines based on ultra-low temperature crushing and deep eutectic solvent synergy according to claim 1, characterized in that: The ultrasonic frequency of the ultrasonic extraction described in step 3 is 350-450W.

5. The method for extracting active ingredients from traditional Chinese medicines based on ultra-low temperature crushing and deep eutectic solvent synergy according to claim 1, characterized in that: The elution described in step 4 is to first remove the deep eutectic solvent by eluting with distilled water, and then eluting with 30%, 60%, 90%, and 100% ethanol in sequence and combining the eluates.

6. A compressed candy for sobering up and protecting the liver, characterized in that: The raw materials include, by weight: 5-15 parts of ganoderma lucidum extract, 5-10 parts of kudzu root extract, 4-8 parts of Hovenia dulcis extract, 5-10 parts of hawthorn extract, 4-5 parts of olive extract, 3-9 parts of xylitol, 1-2 parts of citric acid, 5-10 parts of microcrystalline cellulose, 15-30 parts of maltodextrin, and 0.5-1 part of magnesium stearate; The ganoderma lucidum extract, kudzu root extract, Hovenia dulcis extract, hawthorn extract and olive extract are extracted by the method described in any one of claims 1 to 5.

7. A method for preparing the alcohol-relieving and liver-protecting compressed candy according to claim 6, characterized in that: The following steps are involved: Step 1: Mix the raw materials according to the ratio; Step 2: Place the mixed materials in a stainless steel tray, spray 70% to 80% ethanol until moistened, and then granulate; Step 3, the granules prepared in step 1 are passed through a 20-mesh sieve, dried, and then granulated; Step 4: Add magnesium stearate to the granules obtained in step 3, mix well, and use a single punch tablet press to compress the granules to obtain a candy for sobering up and protecting the liver.

8. The method for preparing the alcohol-relieving and liver-protecting compressed candy according to claim 7, characterized in that: The mixing in step 1 is performed using a V-type mixer with a rotation speed of 10 to 15 rpm for 10 to 20 minutes.

9. The method for preparing the alcohol-relieving and liver-protecting compressed candy according to claim 7, characterized in that: The drying described in step 3 is to place the mixture in a constant temperature drying oven at 50-60° C. for 6-8 hours.

10. The method for preparing the alcohol-relieving and liver-protecting compressed candy according to claim 7, characterized in that: The granulation method described in step 3 is to pass through a 80-100 mesh sieve.

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