External spray for treating wound fly maggot disease of livestock and preparation method of external spray

Abstract

The invention provides a livestock wound fly maggot disease external spray and a preparation method thereof, and belongs to the technical field of livestock pharmaceutical preparations, the external spray comprises the following components by volume: an insecticidal active component: 2-4 parts of diazinon; 0.5 to 1 part of blumea oil; 2-3 parts of peppermint oil; a solvent carrier component: 50-70 parts of xylene; 30 to 50 parts of diesel oil; an emulsifier component: 2-8 parts of an emulsifier; according to the invention, diazine belongs to an organophosphorus insecticide and acaricide, and has a good killing effect on various mites, flies, lice and ticks; the wormwood oil has the effects of resisting allergy, resisting inflammation, activating blood, resisting bacteria, resisting viruses, easing pain, enhancing immunity and resisting oxidation; the blumea oil has anti-inflammatory, analgesic, antibacterial, antiviral and penetration-promoting effects, the insecticidal effect of the diazine can be enhanced by matching the diazine with the blumea oil and the peppermint oil, and meanwhile, the irritation of the diazine to animal skin can be relieved by the blumea oil and the peppermint oil.

Description

Technical Field

[0001] The present invention belongs to the technical field of veterinary pharmaceutical preparations, and specifically relates to an external spray for treating myiasis of livestock wounds and a preparation method thereof. Background Art

[0002] In recent years, a series of studies have been carried out on myiasis of wounds, but there are still deficiencies in the prevention, control and treatment of this disease. Herders often use their hands or pliers to pull out maggots and then apply edible oil, pyrethroid drugs, organophosphorus drugs and medicated bath for treatment. However, it is difficult to immobilize livestock, inconvenient to administer drugs, and the curative effect is not ideal, thus bringing great economic losses to the livestock industry.

[0003] The Chinese patent document with the publication number of CN203087198U discloses a fly repellent for camels. The principle of preventing myiasis of camel vagina is that the fly repellent contains diazinon (diazinon, thiophosphoric acid), and through the release of drug efficacy, it can effectively prevent myiasis of camel vagina.

[0004] Diazinon belongs to organophosphorus insecticides and acaricides, with contact toxicity, stomach toxicity, fumigation and weak systemic effects, and has good killing effects on various mites, flies, lice and ticks. However, its drug efficacy is not significant; at the same time, as an organophosphorus insecticide, diazinon has certain irritation to animal skin, and after animals use it, erythema and edema often occur. Summary of the Invention

[0005] Based on the above technical problems, the present invention provides an external spray for treating myiasis of livestock wounds and a preparation method thereof, specifically as follows: On the one hand, the present invention provides an external spray for treating myiasis of livestock wounds. According to the volume component ratio, the external spray includes: Insecticidal active component: 2 - 4 parts of diazinon; 0.5 - 1 part of artemisia argyi oil; 2 - 3 parts of peppermint oil; Solvent carrier component: 50 - 70 parts of xylene; 30 - 50 parts of diesel; Emulsifier component: 2 - 8 parts of emulsifier; Preferably, according to the volume component ratio, the external spray includes: Insecticidal active component: 4 parts of diazinon; 0.5 part of artemisia argyi oil; 2.5 parts of peppermint oil; Solvent carrier component: 60 parts of xylene; 40 parts of diesel; Emulsifier component: 4 parts of emulsifier.

[0006] Preferably, the emulsifier is polyoxyethylene castor oil EL - 40 or Tween - 80.

[0007] On the other hand, a preparation method of an external spray for treating myiasis of livestock wounds includes the following steps: S1. Preparation: Weigh 2 - 4 volume parts of diazinon; 0.5 - 1 volume part of artemisia argyi oil; 2 - 3 volume parts of menthol oil; 2 - 8 volume parts of emulsifier; 50 - 70 volume parts of xylene; 30 - 50 volume parts of diesel, and place them in different containers for later use; S2. Configuration: Stir diazinon, emulsifier and xylene at 25 - 30 °C at 200 - 300 rpm for 10 - 15 minutes to form a homogeneous phase; S3. Preparation: Add artemisia argyi oil, menthol oil and diesel in sequence. After adding each component, continue to stir for 5 minutes, and finally obtain a light yellow clear liquid by ultrasonic treatment, which is the external spray.

[0008] Preferably, in step S3, the ultrasonic treatment time is 10 minutes and the ultrasonic frequency is 40 kHz.

[0009] In the third aspect, the present invention provides the application of the external spray for treating myiasis in livestock wounds. After spraying, the external spray forms an insecticidal film with strong adhesion on the wound surface, and a single application can maintain the insecticidal effect for 6 - 8 weeks.

[0010] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. In the present invention, diazinon belongs to an organophosphorus insecticide and acaricide, which has contact killing, stomach poisoning, fumigation and weak systemic effects, and has good killing effects on various mites, flies, lice and ticks. After spraying, its adhesion on the skin and hair is very strong, and it can maintain a long-term insecticidal effect; artemisia argyi oil has the effects of anti-allergy, anti-inflammatory and promoting blood circulation, antibacterial, antiviral, analgesic, enhancing immunity and antioxidant; menthol oil has the effects of anti-inflammatory and analgesic, antibacterial and antiviral, and promoting penetration. The combination of diazinon and artemisia argyi oil + menthol oil can enhance the insecticidal effect of diazinon. At the same time, artemisia argyi oil + menthol oil can reduce the irritation of diazinon to the animal skin.

[0011] 2. For the external spray for treating myiasis in livestock wounds provided by the present invention, xylene and diesel are used as slow-release carriers to form a xylene - diesel system, which can form an adhesion film on the animal skin and make the drug effect last for 6 - 8 weeks. Description of the Drawings

[0012] Figure 1 It is the high performance liquid chromatography (HPLC) chart of diazinon in the spray; Figure 2 It is the HPLC chart of the diazinon reference substance; Figure 3 It is the comparison before and after the treatment of a sheep suffering from myiasis; Figure 4 It is the comparison before and after the treatment of a camel suffering from myiasis; Figure 5 It is the effect after the treatment of a sheep suffering from myiasis in Control Group 1. Detailed implementation mode

[0013] Example 1 An external spray for treating myiasis of livestock wounds. In terms of volume component ratio, the external spray includes: Insecticidal active component: 4 parts of diazinon; 1 part of artemisia argyi oil; 3 parts of peppermint oil; Solvent carrier component: 70 parts of xylene; 50 parts of diesel oil; Emulsifier component: 8 parts of emulsifier; Moreover, the emulsifier is Tween-80.

[0014] Moreover, the preparation method of the external spray includes the following steps: S1. Preparation: Place the above-mentioned component medicaments in different containers for later use; S2. Configuration: Stir diazinon, emulsifier and xylene at 25 °C at 200 rpm for 10 minutes to form a homogeneous phase; S3. Preparation: Sequentially add artemisia argyi oil, peppermint oil and diesel oil, continue to stir for 5 minutes after adding each component, and finally obtain a light yellow clear liquid through ultrasonic treatment, which is the external spray.

[0015] Moreover, in step S3, the ultrasonic treatment time is 10 minutes and the ultrasonic frequency is 40 kHz.

[0016] Moreover, after the external spray is sprayed, an insecticidal film with strong adhesion is formed on the wound surface, and the insecticidal effect can be maintained for 6 - 8 weeks with one application.

[0017] Example 2 An external spray for treating myiasis of livestock wounds. In terms of volume component ratio, the external spray includes: Insecticidal active component: 2 parts of diazinon; 0.5 part of artemisia argyi oil; 2 parts of peppermint oil; Solvent carrier component: 50 parts of xylene; 30 parts of diesel oil; Emulsifier component: 2 parts of emulsifier; Moreover, the emulsifier is polyoxyethylene castor oil EL-40.

[0018] Moreover, the preparation method of the external spray includes the following steps: S1. Preparation: Place the above-mentioned component medicaments in different containers for later use; S2. Configuration: Stir diazinon, emulsifier and xylene at 30 °C at 300 rpm for 15 minutes to form a homogeneous phase; S3. Preparation: Sequentially add artemisia argyi oil, peppermint oil and diesel oil, continue to stir for 5 minutes after adding each component, and finally obtain a light yellow clear liquid through ultrasonic treatment, which is the external spray.

[0019] Moreover, in the step S3, the ultrasonic treatment time is 10 minutes and the ultrasonic frequency is 40 kHz.

[0020] Moreover, after the external spray is sprayed, an insecticidal film with strong adhesion is formed on the wound surface, and the insecticidal effect can be maintained for 6 - 8 weeks with one application.

[0021] Example 3 An external spray for treating myiasis of livestock wounds, in terms of volume component ratio, the external spray includes: Insecticidal active component: 4 parts of diazinon; 0.5 part of artemisia argyi oil; 2.5 parts of peppermint oil; Solvent carrier component: 60 parts of xylene; 40 parts of diesel; Emulsifier component: 4 parts of emulsifier; Moreover, the emulsifier is Tween - 80.

[0022] Moreover, the preparation method of the external spray includes the following steps: S1. Preparation: Place the above - mentioned component agents in different containers for later use; S2. Configuration: Stir diazinon, emulsifier and xylene at 28 °C at 250 rpm for 13 minutes to form a homogeneous phase; S3. Preparation: Add artemisia argyi oil, peppermint oil and diesel in sequence, continue to stir for 5 minutes after adding each component, and finally obtain a light - yellow clear liquid through ultrasonic treatment, which is the external spray.

[0023] Moreover, in the step S3, the ultrasonic treatment time is 10 minutes and the ultrasonic frequency is 40 kHz.

[0024] Moreover, after the external spray is sprayed, an insecticidal film with strong adhesion is formed on the wound surface, and the insecticidal effect can be maintained for 6 - 8 weeks with one application.

[0025] Experimental part

[0026] Experiment 1 The content of diazinon in the external spray prepared in Example 3 was detected by liquid chromatography, and the results are shown in Figure 1 、 2 Shown as Figure 1 is the high - performance liquid chromatography of diazinon in the spray, Figure 2 is the chromatogram of the diazinon reference substance (standard solution with diazinon as the main component). It can be seen from the figure that the peak emergence time of the diazinon chromatographic peak in the spray is consistent with that of the reference substance, indicating that the diazinon component in the spray is the same substance as the reference substance, verifying that the chemical structure of diazinon in the spray has not changed. The peak shape of the chromatographic peak is symmetric and single, without obvious impurity peaks.

[0027] Experiment 2 Verification of the Liquid Chromatography Method 1. System Suitability Test Sample Preparation: Take the topical spray solution, dilute it to an appropriate concentration with methanol, and filter it through a 0.45 μm filter membrane to obtain the test solution.

[0028] Repeated Injection: Inject the same test solution continuously for 5 times, and record the chromatographic peak area, resolution (R), and relative standard deviation of peak area (RSD) of diazinon.

[0029] Table 1 System Suitability Test

[0030] As can be seen from Table 1, the RSD of the peak area for 5 injections is 0.37% (far lower than the limit of 2.0%), indicating that the method has good repeatability and high instrument stability. The resolution R of the chromatographic peak from the adjacent peak is > 1.5, meeting the requirements of the analytical method, indicating that the target peak (diazinon) is completely separated from other components without interference. The fluctuation of the peak area for 5 injections is extremely small (2026 - 2043), further proving that the method is stable and reliable.

[0031] 2. Stability Test Take the same batch of topical spray, place it at room temperature, and inject samples at intervals of 0, 2, 4, 6, 12, 24, and 48 h under the chromatographic conditions, and record the peak area of the main drug (diazinon) respectively. The results are shown in Table 2.

[0032] Table 2 Stability Test

[0033] As can be seen from Table 2, within 48 hours, the fluctuation range of the peak area of diazinon is 2058 - 2077, without an obvious downward trend, indicating that the drug components are stable and no degradation has occurred. The RSD at all time points is ≤ 0.57%, far lower than the limit of 2.0%, indicating that the analytical method has high precision and reliable data. Even after 48 hours, the peak area remains stable (RSD is only 0.14%), indicating that short-term storage of the spray at room temperature will not affect the content of diazinon.

[0034] 3. Repeatability Test Take the same batch of topical spray, inject samples continuously for 6 times under the chromatographic conditions, record the peak area of the main drug, and calculate the content of the main drug. The results are shown in Table 3.

[0035] Table 3 Repeatability Test

[0036] As can be seen from Table 3, the diazinon content in the 6 determinations was 97.91% - 98.49%, with an average value of 98.11%, which is close to the theoretical value (100%), indicating that the preparation process is stable. The content RSD = 0.23% (<2.0%), and the peak area RSD = 0.23%, indicating excellent repeatability of the method and extremely small operation errors. The peak area and the content results are highly consistent (for example, the peak area of the 6th time is the highest, and the corresponding content is also slightly higher), further verifying the accuracy of the method.

[0037] 4. Spray Stability Test 4.1 High Temperature Test The same batch of topical spray was placed in a closed vial and put into a stable constant temperature incubator. It was placed at 40°C for 10 days. The appearance of the spray was observed on the 0th, 5th, and 10th days, and the content of the main drug in the spray was detected. The results are shown in Table 4.

[0038] Table 4 Influence of High Temperature at 40°C on the Stability of the Spray

[0039] As can be seen from Table 4, when stored at 40°C for 10 days, the spray always remained clear and light yellow, without precipitation, delamination or discoloration, indicating that the physical properties of the preparation are stable. The content only decreased by 0.46% within 10 days, which is lower than the allowable range of general drug preparations (usually ±5%).

[0040] 4.2 High Humidity Test The same batch of topical spray was placed in a closed vial at a temperature of 25°C and a relative humidity of 90% ± 5%. It was placed for 10 days. The appearance of the spray was observed on the 0th, 5th, and 10th days, and the content of the main drug in the spray was detected. The results are shown in Table 5.

[0041] Table 5 Influence of High Humidity on the Stability of the Spray

[0042] As can be seen from Table 5, when stored at 25°C and 90% ± 5% high humidity for 10 days, the spray always remained clear and light yellow, without turbidity, precipitation or delamination, indicating that the physical properties of the preparation are stable and not significantly affected by humidity. The diazinon content only decreased by 1.04% within 10 days, and the degradation rate is slow, meeting the stability standard of drug preparations (usually requiring the content change <5%).

[0043] 4.3 Strong Light Irradiation Test The same batch of topical spray was placed in a transparent vial and placed at 4500Lx ± 500Lx of light for 10 days. The appearance changes were observed on the 0th, 5th, and 10th days. The results are shown in Table 6.

[0044] Table 6 Influence of strong light conditions on the stability of the spray

[0045] As can be seen from Table 6, after 10 days of strong light irradiation at 4500Lx ± 500Lx, the spray remained clear light yellow, without discoloration, precipitation or stratification, indicating that the preparation had good tolerance to light. The content decreased to 98.75% after 10 days (cumulative decrease of 0.86%), and the degradation rate was slow.

[0046] Experiment 3 Prepare the medicaments in Table 7 below: Table 7 Medicament components

[0047] Test insects: Third-instar larvae of Lucilia sericata (standardized culture in the laboratory, body length 8 ± 1 mm).

[0048] Experimental group design: (10 larvae in each group, repeated 5 times): Treatment method: Evenly spray the liquid medicines of each group in Table 7 on filter papers with a diameter of 9 cm, put them into petri dishes, and seal them after inoculating Lucilia sericata.

[0049] Experimental results: Table 8 Comparison of the killing effects of each group on maggots

[0050] The mortality rates of control groups 1 - 3 and the implementation group were significantly higher than those of the blank group, proving that diazinon, diazinon + artemisia oil, diazinon + peppermint oil, diazinon + artemisia oil + peppermint oil could all achieve the effect of killing Lucilia sericata. However, the mortality rate of the implementation group could reach about 98% in 24 hours and 100% in 48 hours, which was better than those of control groups 1 - 3. It could be proved that artemisia oil + peppermint oil could improve the insecticidal effect of diazinon.

[0051] LT 50 Indicates the median lethal time, that is, the time required for half of the test subjects to die. The LT 50 of the implementation group was the shortest, which was 12.6 hours, significantly shorter than that of the control group, indicating that its lethal speed was the fastest. The time of control groups 2 and 3 was shorter than that of control group 1, proving that diazinon + artemisia oil / peppermint oil was better than diazinon alone, and the effect of diazinon + artemisia oil + peppermint oil was the best.

[0052] Diazinon belongs to the organophosphorus insecticide and acaricide, and has good killing effects on various mites, flies, lice and ticks. After spraying, its adhesion on the skin and fur is very strong, and it can maintain a long-term insecticidal effect; Wormwood oil has the effects of anti-allergy, anti-inflammatory and promoting blood circulation, antibacterial, antiviral, analgesic, enhancing immunity and antioxidant; Peppermint oil has the effects of anti-inflammatory and analgesic, antibacterial and antiviral, and promoting penetration. The combination of diazinon and wormwood oil + peppermint oil can enhance the insecticidal effect of diazinon. At the same time, wormwood oil + peppermint oil can reduce the irritation of diazinon to the animal skin.

[0053] Although the treatment effect of the control group was also relatively significant, after treatment, it was found that there was a red and swollen phenomenon on the animal skin. The effect of control group 1 is shown in Figure 5 .

[0054] Experiment 4 The external spray prepared in Example 3 of the present invention was used to spray and treat sheep and camels suffering from myiasis, and the healing rate was statistically counted. The treatment effect of sheep is shown in Figure 3 , and the treatment effect of camels is shown in Figure 4 . It can be seen from the figure that the effect after treatment with the present invention is obvious, and there is no problem of irritating erythema or swelling on the animal skin. It can be seen from Table 9 that after one administration of the present invention, the larvae died and fell off, and they recovered after one week, and the healing rate was 100%, and the effect was remarkable.

[0055] Table 9 Effect of the external spray after one administration for one week

Claims

1. An external spray for livestock wound myiasis, characterized in that, By volume component ratio, the external spray includes: Insecticidal active component: 2 - 4 parts of diazinon; 0.5 - 1 part of artemisia argyi oil; 2 - 3 parts of peppermint oil; Solvent carrier component: 50 - 70 parts of xylene; 30 - 50 parts of diesel; Emulsifier component: 2 - 8 parts of emulsifier.

2. The external spray for livestock wound myiasis according to claim 1, characterized in that, By volume component ratio, the external spray includes: Insecticidal active component: 4 parts of diazinon; 0.5 part of artemisia argyi oil; 2.5 parts of peppermint oil; Solvent carrier component: 60 parts of xylene; 40 parts of diesel; Emulsifier component: 4 parts of emulsifier.

3. The external spray for livestock wound myiasis according to any one of claims 1 or 2, characterized in that The emulsifier is polyoxyethylene castor oil EL - 40 or Tween - 80.

4. The preparation method of an external spray for livestock wound myiasis according to claim 1, characterized in that, It includes the following steps: S1. Preparation: Weigh 2 - 4 volume parts of diazinon; 0.5 - 1 volume part of artemisia argyi oil; 2 - 3 volume parts of peppermint oil; 2 - 8 volume parts of emulsifier; 50 - 70 volume parts of xylene; 30 - 50 volume parts of diesel, and place them in different containers for use respectively; S2. Configuration: Stir diazinon, emulsifier and xylene at 25 - 30 °C at 200 - 300 rpm for 10 - 15 minutes to form a homogeneous phase; S3. Preparation: Add artemisia argyi oil, peppermint oil and diesel in sequence, continue to stir for 5 minutes after adding each component, and finally obtain a light yellow clear liquid, namely the external spray, by ultrasonic treatment.

5. The preparation method of an external spray for livestock wound myiasis according to claim 4, characterized in that, In the step S3, the ultrasonic treatment time is 10 minutes and the ultrasonic frequency is 40 kHz.

6. Use of the external spray for livestock wound myiasis according to claim 1 or 2, characterized in that, After the external spray is sprayed, an insecticidal film is formed on the wound surface, and the insecticidal effect is maintained for 6 - 8 weeks with one application.

Images