Multi-process composite molding 3D biological printing manufacturing device

Abstract

The invention relates to a 3D biological printing manufacturing device for multi-process composite forming. The 3D biological printing manufacturing device mainly comprises a rack, a spray head printing system and a forming platform system. The printing system can achieve extrusion biological printing and electrostatic spinning and mainly comprises a Y-direction sliding rail, a sliding block, a gantry, an X-direction sliding rail, a magnetic suction cup, an electrostatic spinning spray head, an extrusion printing spray head, a DLP light source and a DLP light source suspension. The forming platform system can move in the Z-axis direction and mainly comprises a table top, a material groove, a Z-direction sliding rail, a receiving platform, a platform clamp, a Z-axis sliding block, a driving device, a square hole and a positioning hole. Compared with a single forming mode, the multi-process composite forming biological manufacturing device can achieve multi-material and multi-scale integrated forming printing of a complex tissue system.

Description

Technical Field

[0001] The present invention relates to a 3D bioprinting manufacturing device with multi-process composite forming. Background Art

[0002] 3D bioprinting is a 3D printing technology that is based on a computer three-dimensional model and locates and assembles biomaterials or living cells by means of software layer-by-layer discretization and numerical control forming to manufacture biomedical products such as medical aids, artificial implant scaffolds, and tissue organs. It is widely applied to research directions such as regenerative medicine, pharmacokinetics, and cell biology. 3D bioprinting is often regarded as an enabling technology for tissue engineering. It uses cell-laden bioinks as basic raw materials to construct tissues and organs with physiological functions for transplantation and repair of damaged tissues.

[0003] Currently, 3D printing technologies for biomanufacturing mainly include extrusion-based bioprinting, digital light processing technology (DLP), stereolithography technology (SLA), electrospinning technology, inkjet bioprinting technology, etc. However, when facing human tissues with complex and diverse compositions and structures, a single forming method cannot achieve multi-material and multi-scale bioprinting, nor can it achieve integrated forming, which cannot meet the actual needs. Summary of the Invention

[0004] In view of this, the present invention discloses a 3D bioprinting manufacturing device with multi-process composite forming. Compared with traditional biomanufacturing devices, the present invention can achieve multi-scale and multi-material composite forming in a single printing process and can achieve integrated forming when printing complex human tissue structures.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A 3D bioprinting manufacturing device with multi-process composite forming, comprising a frame, a nozzle printing system and a forming platform system located on the frame;

[0007] The nozzle printing system can achieve extrusion bioprinting and electrospinning, and mainly includes a Y-direction slide rail, a slider, a gantry, an X-direction slide rail, a magnetic chuck, an electrospinning nozzle, an extrusion printing nozzle, a DLP light source, and a DLP light source cross suspension;

[0008] The forming platform system can move in the Z-axis direction and mainly includes a desktop, a material tank, a Z-direction slide rail, a receiving platform, a platform fixture, a Z-axis slider, and a Z-axis driving device.

[0009] Preferably, the Y-direction slide rail is fixed on the desktop, the slider is located on the Y-direction slide rail, the gantry is fixed on the slider, and the gantry can move along the Y-direction slide rail together with the slider.

[0010] Preferably, an X-direction slide rail is fixed on the gantry, and the magnetic chuck is installed on the X-direction slide rail.

[0011] Preferably, the electrospinning nozzle and the extrusion printing nozzle are fixed on the magnetic chuck by magnetic attraction.

[0012] Preferably, the DLP light source is arranged for upward projection, and the DLP light source is fixed on the DLP light source cross suspension.

[0013] Preferably, the forming platform system is located below the desktop, the material tank is installed on the receiving platform, the receiving platform is fixed on the Z-direction slide rail by a platform clamp, and the Z-axis driving device is installed below the Z-direction slide rail.

[0014] Preferably, a square hole is provided on the desktop, and positioning holes are provided around the square hole.

[0015] Specifically, the working principle of the 3D biological printing manufacturing device with multi-process composite forming provided by the present invention is as follows:

[0016] First, the extrusion nozzle moves along a predetermined path on the X- and Y-direction slide rails, and extrudes various types of biological inks onto the printing platform. When the first layer is extruded, the forming platform descends a certain distance on the Z-direction slide rail, and then the second layer is extruded. After the extrusion process is completed, the extrusion nozzle retreats to an area outside the forming platform, and crosslinking and curing are achieved through an upward projection DLP light source or other physical and chemical methods. Then, photosensitive biological ink is injected into the printing material tank, and the DLP light source projects a specific pattern to selectively cure the photosensitive biological ink. When the first layer of DLP curing is completed, the forming platform descends a certain distance on the Z-direction slide rail, and then the second layer is cured. The electrospinning nozzle can perform interspersed spinning during the layer-by-layer printing process, and the spun fibers are directly embedded and connected to the printed model through the crosslinking of the printing ink.

[0017] Compared with the prior art, the technology of the present invention has the following excellent effects:

[0018] The present invention provides a 3D biological printing manufacturing device with multi-process composite forming. Compared with traditional 3D printing devices, this device integrates extrusion forming, DLP forming technology, and electrospinning forming technology, and can achieve multi-mode and multi-material printing in one printing process, and complete integrated forming during the printing of complex and diverse human tissues. Description of the Drawings

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings described below are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on the provided drawings.

[0020] Figure 1 It is a schematic diagram of the overall structure of the 3D bioprinting manufacturing device with multi-process composite molding according to the present invention;

[0021] Figure 2 It is a schematic diagram of the molding platform structure of the 3D bioprinting manufacturing device with multi-process composite molding according to the present invention;

[0022] Figure 3 It is a schematic diagram of the partial structure of the 3D bioprinting manufacturing device with multi-process composite molding according to the present invention.

[0023] Wherein:

[0024] 1 - Frame; 2 - Y-direction slide rail; 3 - Slide block; 4 - Gantry; 5 - X-direction slide rail; 6 - Electromagnetic chuck; 7 - Electrospinning nozzle; 8, 9, 10 - Extrusion printing nozzles; 11 - DLP light source; 12 - DLP light source cross suspension; 13 - Desktop; 14 - Material tank; 15 - Z-direction slide rail; 16 - Receiving platform; 17 - Platform fixture; 18 - Z-axis slide block; 19 - Z-axis driving device; 20 - Square hole; 21 - Positioning hole. Detailed embodiments

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0026] Here, the special term "embodiment", as any embodiment described as "exemplary", does not have to be interpreted as superior to or better than other embodiments. For the performance index tests in the embodiments of this application, unless otherwise specified, the conventional test methods in the art are used. It should be understood that the terms described in this application are only used to describe specific embodiments and are not used to limit the content disclosed in this application.

[0027] Unless otherwise specified, the technical and scientific terms used herein have the same meanings as those commonly understood by those of ordinary skill in the technical field to which this application belongs; the test methods and technical means not otherwise specifically noted in this application refer to the experimental methods and technical means commonly used by those of ordinary skill in the art.

[0028] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by terms such as "middle", "upper", "lower", "ascending", "descending", "vertical", "surface", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention.

[0029] It should be understood that terms such as "having", "comprising", and "including" as used herein do not preclude the presence or addition of one or more other elements or combinations thereof.

[0030] It should be noted that in the description of the present invention, the orientation or positional relationship indicated by the terms is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention.

[0031] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, terms such as "installed", "provided with", "sheathed / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection. It can be a mechanical connection or an electrical connection. It can be directly connected or indirectly connected through an intermediate medium. It can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0032] Figure 1 and Figure 2 shows a 3D bioprinting manufacturing device for multi-process composite molding disclosed by the present invention. Figure 3 A partial CAD drawing of the device is shown, which includes a cooperating frame, a nozzle printing system, and a forming platform system. It should be noted that in Figure 1 and Figure 2 the equipment shown, the Z-axis is installed below the desktop, Figure 3 and what is shown is the situation of being installed above the desktop.

[0033] The described nozzle printing system can achieve extrusion bioprinting and electrospinning, mainly including a Y-direction slide rail 2, a slider 3, a gantry 4, an X-direction slide rail 5, a magnetic chuck 6, an electrospinning nozzle 7, extrusion printing nozzles 8, 9, 10, a DLP light source 11, and a DLP light source cross suspension 12; the Y-direction slide rail 2 is fixed on a table 13, the slider 3 is located on the Y-direction slide rail 2, the gantry 4 is fixed on the slider 3, and the gantry 4 can move along the Y-direction slide rail 2 together with the slider 3; an X-direction slide rail 5 is fixed on the gantry 4, the magnetic chuck 6 is installed on the X-direction slide rail 5, and the electrospinning nozzle 7 and the extrusion printing nozzles 8, 9, 10 are fixed on the magnetic chuck 6 by magnetic attraction. The DLP light source 11 is arranged for upward projection and is fixed on the DLP light source cross suspension 12. Driven by a motor, the nozzle can achieve sweeping in the XY plane. The extrusion nozzle is filled with bioink and can extrude and print layer by layer according to the sliced data of computer software. To adapt to the characteristics of different bioinks, the extrusion nozzles are divided into a low-temperature extrusion head and a high-temperature extrusion head.

[0034] The forming platform system can move in the Z-axis direction, mainly including a table 13, a material tank 14, a Z-direction slide rail 15, a receiving platform 16, a platform fixture 17, a Z-axis slider 18, and a Z-axis driving device 19; the forming platform system is located below the table 13, the material tank 14 can be installed on the receiving platform 16, the receiving platform 16 is fixed on the Z-direction slide rail 15 through the platform fixture 17, the Z-axis driving device 19 is installed below the Z-axis slide rail, a square hole 20 is provided on the table 13, and positioning holes are provided around the square hole.

[0035] Specific example:

[0036] 1. Check the equipment and turn on the power supply;

[0037] 2. Add bioink to the extrusion printing nozzles, electrospinning nozzle, and material tank;

[0038] 3. Input the three-dimensional model to be printed into the computer software for layer-by-layer slicing; set the temperature of the printing nozzle and the printing platform, set the extrusion speed of the extrusion nozzle, and set the printing layer height during extrusion; set the power and illumination time of the DLP light source, and set the high-voltage parameters during electrospinning. After the parameter setting is completed;

[0039] 4. First, calibrate the positions of the extrusion nozzle and the printing platform. After calibration, first perform extrusion printing. The extrusion nozzle moves above the printing platform, the receiving platform rises. After reaching the specified position, the extrusion nozzle extrudes bioink according to the sliced data. After printing one layer, execute the next layer printing command;

[0040] 5. After layer-by-layer printing is completed, the extrusion nozzle moves to an area outside the printing platform; at this time, the DLP light source cures the already extruded model;

[0041] 6. After the curing is completed, the electrospinning nozzle moves above the printing platform and performs electrospinning operations on the already completed extrusion model. After the electrospinning is finished, the electrospinning nozzle moves to an area outside the printing platform;

[0042] 7. The receiving platform carries the already printed model and immerses it into a trough containing bio-ink. The layer height between the upper surface of the model and the upper surface of the bio-ink is equal to the layer height of DLP curing. Layer by layer curing is performed according to the slicing information until the printing is completed;

[0043] 8. After the printing is completed, the model is removed from the receiving platform, and the residual bio-ink in the nozzle and the trough is cleaned;

[0044] 9. Turn off the power supply.

[0045] The above solution is only an illustration of a preferred example and is not limited thereto. When implementing the present invention, appropriate substitutions and / or modifications can be made according to the needs of users.

[0046] The number of devices and the processing scale described here are used to simplify the description of the present invention. The application, modification, and variation of the present invention are obvious to those skilled in the art.

[0047] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and embodiments. It can be fully applied to various fields suitable for the present invention. For those familiar with the field, additional modifications can be easily achieved. Therefore, without departing from the general concept defined by the claims and the equivalent scope, the present invention is not limited to specific details and the illustrations shown and described here.

[0048] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A 3D bio-printing manufacturing device for multi-process composite forming, characterized in that, The device mainly includes a frame (1), a nozzle printing system and a forming platform system located on the frame (1). The nozzle printing system can realize extrusion bioprinting and electrospinning, and mainly includes a Y-direction slide rail (2), a slider (3), a gantry (4), an X-direction slide rail (5), a magnetic chuck (6), an electrospinning nozzle (7), extrusion printing nozzles (8, 9, 10), a DLP light source (11) and a DLP light source cross suspension (12). The forming platform system moves in the Z-axis direction and mainly includes a table (13), a material tank (14), a Z-direction slide rail (15), a receiving platform (16), a platform fixture (17), a Z-axis slider (18) and a Z-axis driving device (19).

2. The 3D bioprinting manufacturing device for multi-process composite forming according to claim 1, wherein, The Y-direction slide rail (2) is fixed on the table (13), the slider (3) is located on the Y-direction slide rail (2), the gantry (4) is fixed on the slider (3), and the gantry (4) and the slider (3) move along the Y-direction slide rail (2) together.

3. The 3D bioprinting manufacturing device for multi-process composite forming according to claim 2, characterized in that, An X-direction slide rail (5) is fixed on the gantry (4), and the magnetic chuck (6) is installed on the X-direction slide rail (5).

4. The 3D bioprinting manufacturing device for multi-process composite forming according to claim 1, characterized in that, The electrospinning nozzle (7) and the extrusion printing nozzles (8, 9, 10) are fixed on the magnetic chuck (6) by magnetic attraction.

5. The 3D bioprinting manufacturing device for multi-process composite forming according to claim 1, characterized in that, The DLP light source (11) is arranged for upward projection, and the DLP light source (11) is fixed on the DLP light source cross suspension (12).

6. The 3D bioprinting manufacturing device for multi-process composite forming according to claim 1, characterized in that, The forming platform system is located below the table (13), the material tank (14) is installed on the receiving platform (16), the receiving platform (16) is fixed on the Z-direction slide rail (15) by the platform fixture (17), and the Z-axis driving device (19) is installed below the Z-direction slide rail (15).

7. The 3D bioprinting manufacturing device for multi-process composite molding according to claim 6, characterized in that, A square hole (20) is provided on the table (13), and positioning holes (21) are provided around the square hole (20).

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