Cell culture device

Abstract

The invention relates to the technical field of cell culture, in particular to a cell culture device which comprises a base, a culture box, a feeding mechanism, a sterilization mechanism and a sealing mechanism, a box body is mounted at the top end of the base, the culture box is mounted at one end of the interior of the box body, and the feeding mechanism comprises a culture frame and a moving assembly. The sterilization mechanism comprises a contact switch, a telescopic assembly, an abutting assembly and a plurality of ultraviolet lamps, the sealing mechanism comprises a first door opening, a first door plate, a second door opening, a second door plate, a lifting assembly and a driving assembly, and the first door plate and the second door plate can be rapidly switched, so that the incubator is prevented from being always opened in the process of taking out a culture dish; therefore, a large amount of external air enters the incubator, dust and bacteria carried in the air can be effectively prevented from entering the incubator, and the possibility that the incubator is polluted is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and specifically to a cell culture device. Background Art

[0002] A cell culture device is equipment used in a laboratory for culturing, preserving, and propagating cells, providing a controllable environment to promote cell growth and proliferation, and is commonly used in experimental research in fields such as cytology and molecular biology. When culturing cells, first, the cells to be cultured are placed in a culture dish, and then the culture dish containing the cells to be cultured is placed in the culture device.

[0003] To ensure the normal culturing of cells, it is necessary to ensure the cleanliness of the gas inside the incubator, thereby ensuring the internal environment of the incubator. In actual use, when taking and placing the culture dish inside the incubator, the incubator needs to go through two actions of being opened and closed. During the opening and closing process of the incubator, external air will enter the inside of the incubator, which will affect the air quality inside the incubator and the normal culturing of cells; The existing Chinese invention patent with the publication number CN117264766B discloses a cell culture device for medical research. When the door of the box is opened, the nozzle can spray disinfectant on the opening position of the partition, using the disinfectant to disinfect the air at the opening position of the partition. In actual use, the sprayed disinfectant easily falls into the culture dish at the opening position, which will affect the cells in the culture dish and is not conducive to the normal culturing of cells. At the same time, when the door of the box is opened, although the disinfectant can be sprayed to disinfect the air, after the door of the box is opened, the disinfectant stops spraying. At this time, the door of the box is still in the open state, and the external gas containing bacteria and dust will still enter the incubator, affecting the internal environment of the incubator. In view of the above problems, a cell culture device is now provided. Summary of the Invention

[0004] The purpose of the present invention is to provide a cell culture device to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solutions: A cell culture device includes a base, and a box body is installed at the top of the base. It further includes an incubator, a feeding mechanism, a sterilization mechanism, and a sealing mechanism; The incubator is installed at one end inside the box body; The feeding mechanism includes a culture rack and a moving component. The culture rack is located inside the incubator, and the moving component is located between the base and the culture rack; The sterilization mechanism includes a contact switch, a telescopic component, a resistance component and a plurality of ultraviolet lamps, wherein the plurality of ultraviolet lamps are installed on the inner wall of the incubator, the contact switch is located on the outer side of the box, the telescopic component is located between the contact switch and the box, and the resistance component is installed on one side of the moving component; The sealing mechanism includes a first door opening, a first door panel, a second door opening, a second door panel, a lifting assembly and a driving assembly. The first door opening is opened on a side of the box body away from the incubator, and the second door opening is opened on a side of the incubator body close to the first door opening. The first door panel is located on one side of the first door opening and is slidably set on the inner wall of the box body. The second door panel is located on one side of the second door opening and is slidably set on the surface of the incubator.

[0006] As a further solution of the present invention: a guide seat is fixed on the top of the base, a movable seat is slidably arranged above the guide seat, and the culture rack is fixedly installed on the top of the movable seat.

[0007] As a further solution of the present invention: the moving component includes an electric push rod, a first connecting rod and a pushing rod, the pushing rod passes through the box body and the incubator and is slidably connected to the box body and the incubator, one end of the pushing rod is fixedly connected to the moving seat, the electric push rod is installed at the bottom end of the base, the output end of the electric push rod is connected to the first connecting rod, and the other end of the pushing rod is connected to the top end of the first connecting rod.

[0008] As a further solution of the present invention: the resistance assembly includes a second connecting rod, a telescopic rod, a first side plate and a contact, the first side plate is fixed to the outer wall of the box, the telescopic rod is slidably arranged on the first side plate, one end of the telescopic rod is fixedly connected to the contact, the other end of the telescopic rod is connected to one end of the second connecting rod, and the other end of the second connecting rod is connected to the first connecting rod.

[0009] As a further solution of the present invention: the telescopic assembly includes a second side plate, a limit plate, a first spring, a guide rail, a moving block and a sliding rod, the second side plate is located on one side of the first side plate and is fixed to the outer wall of the box, the sliding rod is slidably arranged on the second side plate, the contact switch is installed at one end of the sliding rod, the limit plate is installed at the other end of the sliding rod, the first spring is sleeved on the outer wall of the sliding rod, the two ends of the first spring are respectively connected to the limit plate and the second side plate, the guide rail is fixed to the outer wall of the box, the moving block is slidably arranged inside the guide rail, and the top of the moving block is fixedly connected to the contact.

[0010] As a further solution of the present invention: two moving rods are fixedly connected to the top ends of the first door panel and the second door panel, the other end of each moving rod passes through the box body, and a moving plate is fixed to the top ends of the two moving rods on the same side.

[0011] As a further solution of the present invention: The lifting assembly includes a rotating shaft, two vertical rods, two swing rods, two first insertion rods, two first fixing seats, two second insertion rods and two second fixing seats. The two vertical rods are both fixed to the top end of the base. The rotating shaft is rotatably arranged between the two vertical rods. The two swing rods are respectively fixed to the two ends of the rotating shaft. One fixing plate is fixed to one side of each vertical rod. One guide rod is slidably connected to each fixing plate. The top end of each guide rod is connected to a second fixing seat. Each first fixing seat is fixed to the top end of a moving plate. Each first insertion rod is inserted into the inside of a first fixing seat. Each second insertion rod is inserted into the inside of a second fixing seat. Each first insertion rod and a second insertion rod are respectively fixed to the two ends of a swing rod.

[0012] As a further solution of the present invention: The driving assembly includes a mounting plate, a motor, a rotating rod and two hinged rods. The mounting plate is fixedly installed on the top end of the box body. The rotating rod is rotatably arranged on one side of the mounting plate. The motor is installed on the side of the mounting plate away from the rotating rod. The output end of the motor is fixedly connected to the rotating rod. One end of each hinged rod is hinged to the bottom end of a second fixing seat. The other end of each hinged rod is hinged to one end of the rotating rod.

[0013] As a further solution of the present invention: Avoidance grooves are respectively formed in the middle of the bottom ends of the first door panel and the second door panel. One closing assembly is provided on one side of each avoidance groove.

[0014] As a further solution of the present invention: Each closing assembly includes a telescopic plate, a baffle plate, two guide rods and two second springs. The baffle plate is installed on one side of the first door panel. The telescopic plate is located on one side of the avoidance groove and is in contact with the first door panel. The two guide rods are symmetrically and slidably arranged on the baffle plate. The bottom end of each guide rod is fixedly connected to the telescopic plate. Each second spring is sleeved on a guide rod. The two ends of each second spring are respectively connected to the baffle plate and the telescopic plate.

[0015] Compared with the prior art, the beneficial effects of the present invention are: 1. A cell culture device of the present invention, by providing a sealing mechanism, utilizes a first door panel and a second door panel that are opened alternately, when it is necessary to take out the culture dish inside the incubator, by opening the second door panel, at this time the first door panel moves downward to quickly close the first door opening, when the second door panel moves upward and the second door opening is opened, the culture rack is pushed out from the inside of the incubator to the inside of the box body through a feeding assembly, and then the second door panel moves downward to close the second door opening to keep the inside of the incubator sealed, at this time the first door panel moves upward to open the first door opening, and then the culture rack is pushed out of the box body through the feeding assembly, so that the culture dish on the culture rack can be taken out conveniently, and the first door panel and the second door panel can be quickly switched, so as to prevent the incubator from being opened all the time during the process of taking out the culture dish, resulting in a large amount of outside air entering the incubator, which can effectively prevent dust and bacteria carried in the air from entering the incubator, and reduce the possibility of the incubator being contaminated.

[0016] 2. A cell culture device of the present invention, by setting a sterilization mechanism, can use multiple ultraviolet lamps to disinfect and sterilize the interior of the incubator, thereby ensuring the environment inside the incubator; at the same time, through the resistance component and the telescopic component, when the culture rack moves from the incubator toward the interior of the box, the contact does not contact the contact switch, and the ultraviolet lamp does not work at this time; when the culture rack moves from the interior of the incubator into the interior of the box, the contact contacts the contact switch, and the ultraviolet lamp is turned on at this time, that is, after the culture rack leaves the interior of the incubator, the ultraviolet lamp can disinfect and sterilize the interior of the incubator, and can effectively prevent ultraviolet rays from affecting the cells in the culture dish; when the culture rack continues to move out of the interior of the box, the contact drives the contact switch to move, and the ultraviolet lamp is in the on state.

[0017] 3. In a cell culture device of the present invention, when the culture rack needs to enter the interior of the incubator again, the culture rack first enters the interior of the box. At this time, under the action of the first spring, the contact switch can be driven to move, that is, during the process of the culture rack moving from the outside of the box into the interior of the box, the contact is always in contact with the contact switch, and the ultraviolet lamp works. When the culture rack enters the interior of the box and moves toward the incubator, the contact is disengaged from the contact switch, and the ultraviolet lamp does not work at this time, which can effectively prevent ultraviolet rays from affecting the cells in the culture dish. At the same time, the interior of the incubator can be sterilized before the culture dish on the culture rack enters the incubator.

[0018] 4. A cell culture device of the present invention forms a linkage effect between the sterilization mechanism and the feeding mechanism by arranging a telescopic component and a resistance component, that is, when the culture rack is inside the incubator, the ultraviolet lamp does not work to prevent affecting the cells in the culture dish on the culture rack; when the culture rack is moved out of the incubator, the ultraviolet lamp starts to work to disinfect and sterilize the interior of the incubator with high synchronization, which can provide a favorable environment for cell culture and is conducive to the normal progress of cell culture. Brief Description of the Drawings

[0019] Figure 1 This is a schematic structural diagram of the present invention Figure 1 .

[0020] Figure 2 This is a schematic structural diagram of the present invention Figure 2 .

[0021] Figure 3 This is for the present invention Figure 2 an enlarged schematic structural diagram of part A.

[0022] Figure 4 This is a schematic exploded view of the box body in the present invention

[0023] Figure 5 This is a schematic sectional view of the box body and the incubator in the present invention

[0024] Figure 6 This is a schematic exploded view of the box body and the incubator in the present invention

[0025] Figure 7 This is a schematic structural diagram of the first door panel and the second door panel in the present invention

[0026] Figure 8 This is a schematic structural diagram of the swing rod in the present invention

[0027] Figure 9 This is for the present invention Figure 8 an enlarged schematic structural diagram of part B.

[0028] Figure 10 This is a schematic structural diagram of the telescopic plate in the present invention

[0029] Wherein: 11, base; 12, box body; 13, first door opening; 14, first door panel; 15, second door opening; 16, second door panel; 17, moving rod; 18, moving plate; 19, incubator; 21, vertical rod; 22, rotating shaft; 23, swing rod; 24, first insertion rod; 25, first fixed seat; 26, second insertion rod; 27, second fixed seat; 28, guide rod; 29, fixing plate; 30, mounting plate; 31, motor; 32, rotating rod; 33, articulated rod; 34, guiding seat; 35, moving seat; 36, culture rack; 37, electric push rod; 38, first connecting rod; 39, pushing rod; 40, second connecting rod; 41, telescopic rod; 42, first side plate; 43, contact head; 44, sliding rod; 45, second side plate; 46, limiting plate; 47, first spring; 48, guide rail; 49, moving block; 50, contact switch; 51, ultraviolet lamp; 53, avoidance groove; 54, telescopic plate; 55, baffle; 56, guiding rod; 57, second spring. Detailed Description of the Invention

[0030] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0031] The present invention provides the following preferred embodiments: Embodiment 1, as Figures 1 - 10 As shown, a cell culture device includes a base 11, a box 12 is installed on the top of the base 11, and also includes a culture box 19, a feeding mechanism, a sterilization mechanism and a sealing mechanism; The incubator 19 is installed at one end of the inner part of the box body 12. Specifically, the incubator 19 is made of corrosion-resistant materials, and a temperature control system, a humidity control system, a gas control system and a filtration system are installed on the incubator 19. The temperature control system includes a sensor, a heating element and a control panel for setting and adjusting the temperature. The humidity control system maintains a high humidity environment through a humidifier or a water tray. The gas control system is equipped with a CO2 sensor and a gas supply device. Some models are also equipped with an O2 control function. The filtration system is used to purify the air in the box to prevent pollution. The incubator 19 is a prior art and will not be described in detail here. The feeding mechanism includes a culture rack 36 and a moving assembly. The culture rack 36 is located inside the incubator 19. The moving assembly is located between the base 11 and the culture rack 36. In actual use, when cells need to be cultured, a culture dish containing cells to be cultured is placed on the culture rack 36, and the culture rack 36 is moved into the incubator 19 by using the moving assembly, thereby achieving cell culture. The sterilization mechanism includes a contact switch 50, a telescopic component, a resistance component and a plurality of ultraviolet lamps 51, wherein the plurality of ultraviolet lamps 51 are installed on the inner wall of the incubator 19, the contact switch 50 is located on the outer side of the box 12, the telescopic component is located between the contact switch 50 and the box 12, and the resistance component is installed on one side of the moving component; When the contact 43 contacts the contact switch 50, the plurality of second door openings 15 are opened to achieve sterilization of the interior of the incubator 19; The sealing mechanism includes a first door hole 13, a first door panel 14, a second door hole 15, a second door panel 16, a lifting assembly and a driving assembly. The first door hole 13 is opened on a side of the box body 12 away from the incubator 19, and the second door hole 15 is opened on a side of the incubator 19 close to the first door hole 13. Both the first door hole 13 and the second door hole 15 are used for the culture rack 36 to pass through. The first door panel 14 is located at one side of the first door hole 13 and is slidably set on the inner wall of the box body 12. The first door panel 14 is used to achieve blocking of the first door hole 13. The second door panel 16 is located at one side of the second door hole 15 and is slidably set on the surface of the incubator 19. The second door panel 16 is used to achieve blocking of the second door hole 15. In the initial state, the second door panel 16 blocks the second door opening 15 , the first door opening 13 is open, and the culture rack 36 is located inside the incubator 19 . It should be noted that the bottom ends of the first door panel 14 and the second door panel 16 are both provided with grooves for avoiding the guide seat 34 .

[0032] like Figures 1 - 10 As shown, a guide seat 34 is fixed on the top of the base 11, a movable seat 35 is slidably arranged above the guide seat 34, and a culture rack 36 is fixedly installed on the top of the movable seat 35. The sliding connection between the guide seat 34 and the movable seat 35 can provide guidance for the movement of the culture rack 36.

[0033] like Figures 1 - 10 As shown, the moving assembly includes an electric push rod 37, a first connecting rod 38 and a pushing rod 39. The pushing rod 39 penetrates the box body 12 and the incubator 19 and is slidably connected to the box body 12 and the incubator 19, that is, the box body 12 and the incubator 19 are both provided with through holes, and the pushing rod 39 is slidably arranged inside the through holes. It should be noted that a sealing ring is provided on the inner wall of the through hole, one end of the pushing rod 39 is fixedly connected to the moving seat 35, the electric push rod 37 is installed at the bottom end of the base 11, the output end of the electric push rod 37 is connected to the first connecting rod 38, and the other end of the pushing rod 39 is connected to the top end of the first connecting rod 38; When the culture rack 36 needs to be moved out, the controller controls the electric push rod 37 to work, and the electric push rod 37 can drive the first connecting rod 38 to move toward the direction close to the box body 12. The first connecting rod 38 drives the culture rack 36 and the movable seat 35 to slide on the guide seat 34 through the pushing rod 39, so that the culture rack 36 can be moved out.

[0034] like Figures 1 - 10 As shown, the abutment assembly includes a second connecting rod 40, a telescopic rod 41, a first side plate 42 and a contact 43, the first side plate 42 is fixed to the outer wall of the box body 12, the telescopic rod 41 is slidably arranged on the first side plate 42, one end of the telescopic rod 41 is fixedly connected to the contact 43, the other end of the telescopic rod 41 is connected to one end of the second connecting rod 40, and the other end of the second connecting rod 40 is connected to the first connecting rod 38; When the first connecting rod 38 moves toward the box body 12 , the first connecting rod 38 drives the telescopic rod 41 to slide on the first side plate 42 through the second connecting rod 40 , and the telescopic rod 41 can drive the contact 43 to move in the same direction when moving.

[0035] like Figures 1 - 10As shown, the telescopic assembly includes a second side plate 45, a limit plate 46, a first spring 47, a guide rail 48, a moving block 49 and a sliding rod 44. The second side plate 45 is located at one side of the first side plate 42 and is fixed to the outer wall of the box body 12. The sliding rod 44 is slidably arranged on the second side plate 45. The contact switch 50 is installed at one end of the sliding rod 44. The limit plate 46 is installed at the other end of the sliding rod 44. The first spring 47 is sleeved on the outer wall of the sliding rod 44. The two ends of the first spring 47 are respectively connected to the limit plate 46 and the second side plate 45. The guide rail 48 is fixed to the outer wall of the box body 12. The moving block 49 is slidably arranged inside the guide rail 48. The top end of the moving block 49 is fixedly connected to the contact 43. When the culture rack 36 moves from the incubator 19 to the inside of the box 12, the contact 43 moves toward the direction close to the contact switch 50, but does not contact the contact switch 50. At this time, the ultraviolet lamp 51 does not work. When the culture rack 36 moves from the inside of the incubator 19 to the inside of the box 12, the contact 43 is moved to one side of the contact switch 50 and contacts the contact switch 50. At this time, the ultraviolet lamp 51 is turned on, that is, after the culture rack 36 leaves the inside of the incubator 19, the ultraviolet lamp 51 can disinfect and sterilize the inside of the incubator 19, which can effectively prevent ultraviolet rays from affecting the cells in the culture dish. When the culture rack 36 continues to move out of the box 12, the contact 43 pushes the contact switch 50 to move, and the contact switch 50 drives the moving block 49 to slide inside the guide rail 48. At the same time, the contact switch 50 drives the sliding rod 44 to slide on the second side plate 45. When the sliding rod 44 moves, it drives the limit plate 46 to move, and the first spring 47 is stretched, and the ultraviolet lamp 51 is still in the on state. When the culture rack 36 needs to enter the interior of the incubator 19 again, the electric push rod 37 drives the first connecting rod 38 to move away from the box 12, and the first connecting rod 38 drives the telescopic rod 41 to move through the second connecting rod 40, and the telescopic rod 41 drives the contact 43 to move, so that the culture rack 36 first enters the interior of the box 12. In this process, under the action of the first spring 47, the contact switch 50 can be driven to move toward the direction close to the contact 43 and contact the contact 43. That is, when the culture rack 36 moves from the outside of the box 12 to the inside of the box 12, the contact 43 43 is always in contact with the contact switch 50, and the ultraviolet lamp 51 is working. When the push rod 39 continues to move in the direction away from the box 12, so that the culture rack 36 enters the interior of the box 12 and moves toward the incubator 19, the telescopic rod 41 drives the contact 43 to move in the direction away from the contact switch 50, and the contact 43 is separated from the contact switch 50. At this time, the ultraviolet lamp 51 does not work, which can effectively prevent ultraviolet rays from affecting the cells in the culture dish, and at the same time, the interior of the incubator 19 can be sterilized before the culture dish on the culture rack 36 enters the incubator 19; That is, when the culture rack 36 is inside the incubator 19, the ultraviolet lamp 51 does not work to prevent affecting the cells in the culture dish on the culture rack 36. After the culture rack 36 is removed from the inside of the incubator 19, the ultraviolet lamp 51 starts to work to disinfect and sterilize the inside of the incubator 19 with high synchronism, which can provide a favorable environment for cell culture and is conducive to the normal progress of cell culture.

[0036] As Figures 1 - 10 shown, two moving rods 17 are fixedly connected to the top ends of the first door panel 14 and the second door panel 16 respectively. The other end of each moving rod 17 passes through the box body 12, and a moving plate 18 is fixed to the top ends of the two moving rods 17 on the same side.

[0037] As Figures 1 - 10 shown, the lifting assembly includes a rotating shaft 22, two vertical rods 21, two swing rods 23, two first insertion rods 24, two first fixing seats 25, two second insertion rods 26 and two second fixing seats 27. The two vertical rods 21 are both fixed to the top end of the base 11. The rotating shaft 22 is rotatably arranged between the two vertical rods 21. The two swing rods 23 are respectively fixed to the two ends of the rotating shaft 22. A fixing plate 29 is fixed to one side of each vertical rod 21. A guide rod 28 is slidably connected to each fixing plate 29. The top end of each guide rod 28 is connected to a second fixing seat 27. The sliding connection between the guide rod 28 and the fixing plate 29 can play a guiding role for the second fixing seat 27. Each first fixing seat 25 is fixed to the top end of a moving plate 18. Each first insertion rod 24 is inserted into the inside of a first fixing seat 25. Each second insertion rod 26 is inserted into the inside of a second fixing seat 27. Each first insertion rod 24 and a second insertion rod 26 are respectively fixed to the two ends of a swing rod 23; Specifically, a strip-shaped groove is formed in the middle of each second fixing seat 27 and each first fixing seat 25. The second insertion rod 26 is inserted into the strip-shaped groove in the middle of the second fixing seat 27, and the first insertion rod 24 is inserted into the strip-shaped groove in the middle of the first fixing seat 25.

[0038] As Figures 1 - 10 shown, the driving assembly includes a mounting plate 30, a motor 31, a rotating rod 32 and two hinge rods 33. The mounting plate 30 is fixedly installed on the top end of the box body 12. The rotating rod 32 is rotatably arranged on one side of the mounting plate 30. The motor 31 is installed on the side of the mounting plate 30 away from the rotating rod 32. The output end of the motor 31 is fixedly connected to the rotating rod 32. One end of each hinge rod 33 is hinged to the bottom end of a second fixing seat 27, and the other end of each hinge rod 33 is hinged to one end of the rotating rod 32; When it is necessary to take out the culture dish inside the incubator 19, the controller controls the motor 31 to work, and the motor 31 drives the rotating rod 32 to rotate. At this time, under the action of the two hinge rods 33, the two second fixed seats 27 will move in the direction away from each other, so that the two second insertion rods 26 can drive the two swing rods 23 to rotate reversely around the axis of the rotating shaft 22. When the two swing rods 23 rotate reversely, the two moving plates 18 can be driven by the two first insertion rods 24 and the two first fixed seats 25. At this time, one of the moving plates 18 drives the second door panel 16 to move upward through the moving rod 17, and the other moving plate 18 drives the first door panel 14 to move downward through the moving rod 17; When the second door panel 16 moves upward, the first door panel 14 moves downward to quickly close the first door opening 13. When the second door panel 16 moves upward and the second door opening 15 is opened, the culture rack 36 is pushed out from the inside of the incubator 19 to the inside of the box body 12 through the feeding assembly. Subsequently, the motor 31 controls the rotating rod 32 to rotate reversely, and the second door panel 16 moves downward to close the second door opening 15 to maintain the seal inside the incubator 19. At this time, the first door panel 14 moves upward to open the first door opening 13, and then the culture rack 36 is pushed out of the box body 12 through the feeding assembly, which is convenient for taking out the culture dish on the culture rack 36. The first door panel 14 and the second door panel 16 can be quickly switched, so as to prevent the incubator 19 from being always opened during the process of taking out the culture dish, resulting in a large amount of external air entering the incubator 19, effectively preventing the dust and bacteria carried in the air from entering the incubator 19, and reducing the possibility of the incubator 19 being contaminated.

[0039] As Figures 1 - 10 shown, avoidance grooves 53 are provided in the middle of the bottom ends of the first door panel 14 and the second door panel 16. A closing assembly is provided on one side of each avoidance groove 53. Specifically, the size of the avoidance groove 53 matches the size of the push rod 39, and the avoidance groove 53 plays a role in avoidance to prevent the push rod 39 from conflicting with the first door panel 14 or the second door panel 16.

[0040] As Figures 1 - 10 shown, each closing assembly includes a telescopic plate 54, a baffle 55, two guide rods 56 and two second springs 57. The baffle 55 is installed on one side of the first door panel 14. The telescopic plate 54 is located on one side of the avoidance groove 53 and is in contact with the first door panel 14. Similarly, the baffle 55 in the closing assembly on one side of the second door panel 16 is fixed on one side of the second door panel 16. The telescopic plate 54 is located on one side of the avoidance groove 53 and is in contact with the second door panel 16. The two guide rods 56 are symmetrically and slidably arranged on the baffle 55. The bottom end of each guide rod 56 is fixedly connected to the telescopic plate 54. Each second spring 57 is sleeved on a guide rod 56. The two ends of each second spring 57 are respectively connected to the baffle 55 and the telescopic plate 54. The telescopic plate 54 is used to block the avoidance groove 53 to improve the sealing effect; In the initial state, the second spring 57 is in a natural state. When the first door panel 14 or the second door panel 16 conflicts with the push rod 39 during the downward movement, the push rod 39 pushes the telescopic plate 54 to move upward. At this time, the guide rod 56 slides on the baffle 55, and the second spring 57 is compressed, that is, the telescopic plate 54 releases the blockage of the avoidance groove 53, so that the push rod 39 can pass smoothly. At the same time, under the action of the avoidance groove 53, the bottom end of the first door panel 14 or the second door panel 16 can still contact the guide seat 34, thereby achieving a sealing effect.

[0041] The specific working process of the present invention is as follows: When the culture dish on the culture rack 36 needs to be taken out, the controller controls the motor 31 to work, and the motor 31 drives the rotating rod 32 to rotate. At this time, under the action of the two hinged rods 33, the two second fixed seats 27 will move in the direction away from each other, so that the two second plug rods 26 can drive the two swing rods 23 to rotate in the opposite direction around the axis of the rotating shaft 22. When the two swing rods 23 rotate in the opposite direction, the two first plug rods 24 and the two first fixed seats 25 can drive the two movable plates 18 to move. At this time, one of the movable plates 18 drives the second door panel 16 to move upward through the moving rod 17, and the other movable plate 18 drives the first door panel 14 to move downward through the moving rod 17, that is, when the second door panel 16 moves upward, the first door panel 14 moves downward to quickly close the first door opening 13. When the second door panel 16 moves upward and the second door opening 15 is opened, the controller controls the electric push rod 37 to work, and the electric push rod 37 can drive the first The connecting rod 38 moves toward the direction approaching the box body 12, and the first connecting rod 38 drives the culture rack 36 and the moving seat 35 to slide on the guide seat 34 through the pushing rod 39, so that the culture rack 36 can be pushed out from the inside of the incubator 19 to the inside of the box body 12, and then the motor 31 controls the rotating rod 32 to rotate in the opposite direction, and the second door panel 16 moves downward to close the second door opening 15 to keep the inside of the incubator 19 sealed. At this time, the first door panel 14 moves upward to open the first door opening 13, and then the culture rack 36 is pushed out of the box body 12 through the feeding assembly, so that the culture dish on the culture rack 36 can be taken out conveniently. The first door panel 14 and the second door panel 16 can be quickly switched, so as to prevent the incubator 19 from being opened all the time during the process of taking out the culture dish, causing a large amount of outside air to enter the incubator 19, which can effectively prevent dust and bacteria carried in the air from entering the incubator 19, and reduce the possibility of the incubator 19 being contaminated; When the culture rack 36 needs to be put back into the incubator 19 to culture the cells to be cultured, the culture rack 36 is first put into the box body 12 in the same manner, and then the first door panel 14 is closed, and the second door panel 16 is opened so that the culture rack 36 can enter the incubator 19, and then the second door panel 16 is closed; When the culture rack 36 moves from the incubator 19 to the inside of the box 12, the contact 43 moves toward the direction close to the contact switch 50, but does not contact the contact switch 50. At this time, the ultraviolet lamp 51 does not work. When the culture rack 36 moves from the inside of the incubator 19 to the inside of the box 12, the contact 43 is moved to one side of the contact switch 50 and contacts the contact switch 50. At this time, the ultraviolet lamp 51 is turned on, that is, after the culture rack 36 leaves the inside of the incubator 19, the ultraviolet lamp 51 can disinfect and sterilize the inside of the incubator 19, which can effectively prevent ultraviolet rays from affecting the cells in the culture dish. When the culture rack 36 continues to move out of the box 12, the contact 43 pushes the contact switch 50 to move, and the contact switch 50 drives the moving block 49 to slide inside the guide rail 48. At the same time, the contact switch 50 drives the sliding rod 44 to slide on the second side plate 45. When the sliding rod 44 moves, it drives the limit plate 46 to move, and the first spring 47 is stretched, and the ultraviolet lamp 51 is still in the on state. When the culture rack 36 needs to enter the interior of the incubator 19 again, the electric push rod 37 drives the first connecting rod 38 to move away from the box 12, and the first connecting rod 38 drives the telescopic rod 41 to move through the second connecting rod 40, and the telescopic rod 41 drives the contact 43 to move, so that the culture rack 36 first enters the interior of the box 12. In this process, under the action of the first spring 47, the contact switch 50 can be driven to move toward the direction close to the contact 43 and contact the contact 43. That is, when the culture rack 36 moves from the outside of the box 12 to the inside of the box 12, the contact 43 43 is always in contact with the contact switch 50, and the ultraviolet lamp 51 is working. When the push rod 39 continues to move in the direction away from the box 12, so that the culture rack 36 enters the interior of the box 12 and moves toward the incubator 19, the telescopic rod 41 drives the contact 43 to move in the direction away from the contact switch 50, and the contact 43 is separated from the contact switch 50. At this time, the ultraviolet lamp 51 does not work, which can effectively prevent ultraviolet rays from affecting the cells in the culture dish, and at the same time, the interior of the incubator 19 can be sterilized before the culture dish on the culture rack 36 enters the incubator 19; That is, when the culture rack 36 is inside the incubator 19, the ultraviolet lamp 51 does not work to prevent affecting the cells in the culture dish on the culture rack 36. When the culture rack 36 is moved out of the incubator 19, the ultraviolet lamp 51 starts to work to disinfect and sterilize the inside of the incubator 19 with high synchronization, which can provide a favorable environment for cell culture and is conducive to the normal progress of cell culture.

[0042] The beneficial effects of the present invention are specifically embodied in that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture device, comprising a base (11), and a box body (12) is installed at the top of the base (11), characterized in that, Also included is an incubator (19), a feeding mechanism, a sterilizing mechanism and a sealing mechanism; The incubator (19) is installed at one end of the interior of the box (12); The feeding mechanism comprises a culture rack (36) and a moving assembly, wherein the culture rack (36) is located inside the culture box (19), and the moving assembly is located between the base (11) and the culture rack (36); The sterilization mechanism comprises a contact switch (50), a telescopic component, a resistance component and a plurality of ultraviolet lamps (51), wherein the plurality of ultraviolet lamps (51) are all mounted on the inner wall of the incubator (19), the contact switch (50) is located outside the box body (12), the telescopic component is located between the contact switch (50) and the box body (12), and the resistance component is mounted on one side of the moving component; The sealing mechanism comprises a first door opening (13), a first door panel (14), a second door opening (15), a second door panel (16), a lifting assembly and a driving assembly. The first door opening (13) is opened on a side of the box body (12) away from the incubator (19), the second door opening (15) is opened on a side of the incubator (19) close to the first door opening (13), the first door panel (14) is located on one side of the first door opening (13) and is slidably arranged on the inner wall of the box body (12), and the second door panel (16) is located on one side of the second door opening (15) and is slidably arranged on the surface of the incubator (19).

2. The cell culture device according to claim 1, wherein, A guide seat (34) is fixed on the top of the base (11), a movable seat (35) is slidably arranged above the guide seat (34), and a culture rack (36) is fixedly mounted on the top of the movable seat (35).

3. The cell culture device according to claim 2, characterized in that, The moving assembly comprises an electric push rod (37), a first connecting rod (38) and a pushing rod (39); the pushing rod (39) penetrates the box (12) and the incubator (19) and is slidably connected to the box (12) and the incubator (19); one end of the pushing rod (39) is fixedly connected to the moving seat (35); the electric push rod (37) is mounted on the bottom end of the base (11); the output end of the electric push rod (37) is connected to the first connecting rod (38); and the other end of the pushing rod (39) is connected to the top end of the first connecting rod (38).

4. The cell culture device according to claim 3, characterized in that, The abutment assembly comprises a second connecting rod (40), a telescopic rod (41), a first side plate (42) and a contact (43); the first side plate (42) is fixed to the outer wall of the box body (12); the telescopic rod (41) is slidably arranged on the first side plate (42); one end of the telescopic rod (41) is fixedly connected to the contact (43); the other end of the telescopic rod (41) is connected to one end of the second connecting rod (40); and the other end of the second connecting rod (40) is connected to the first connecting rod (38).

5. A cell culture device according to claim 4, characterized in that The telescopic component includes a second side plate (45), a limiting plate (46), a first spring (47), a guide rail (48), a moving block (49) and a sliding rod (44). The second side plate (45) is located on one side of the first side plate (42) and is fixed to the outer wall of the box body (12). The sliding rod (44) is slidably arranged on the second side plate (45). The contact switch (50) is installed at one end of the sliding rod (44). The limiting plate (46) is installed at the other end of the sliding rod (44). The first spring (47) is sleeved on the outer wall of the sliding rod (44). The two ends of the first spring (47) are respectively connected to the limiting plate (46) and the second side plate (45). The guide rail (48) is fixed to the outer wall of the box body (12). The moving block (49) is slidably arranged inside the guide rail (48). The top end of the moving block (49) is fixedly connected to the contact head (43).

6. The cell culture device according to claim 5, wherein, Two moving rods (17) are fixedly connected to the top ends of the first door panel (14) and the second door panel (16). The other end of each moving rod (17) passes through the box body (12), and a moving plate (18) is fixed to the top ends of the two moving rods (17) on the same side.

7. The cell culture device according to claim 6, characterized in that, The lifting component includes a rotating shaft (22), two vertical rods (21), two swing rods (23), two first insertion rods (24), two first fixing seats (25), two second insertion rods (26) and two second fixing seats (27). The two vertical rods (21) are both fixed to the top end of the base (11). The rotating shaft (22) is rotatably arranged between the two vertical rods (21). The two swing rods (23) are respectively fixed to the two ends of the rotating shaft (22). A fixing plate (29) is fixed to one side of each vertical rod (21). A guide rod (28) is slidably connected to each fixing plate (29). The top end of each guide rod (28) is connected to a second fixing seat (27). Each first fixing seat (25) is fixed to the top end of a moving plate (18). Each first insertion rod (24) is inserted into the inside of a first fixing seat (25). Each second insertion rod (26) is inserted into the inside of a second fixing seat (27). Each first insertion rod (24) and a second insertion rod (26) are respectively fixed to the two ends of a swing rod (23).

8. A cell culture device according to claim 7, wherein, The driving component includes a mounting plate (30), a motor (31), a rotating rod (32) and two hinge rods (33). The mounting plate (30) is fixedly installed at the top end of the box body (12). The rotating rod (32) is rotatably arranged on one side of the mounting plate (30). The motor (31) is installed on the side of the mounting plate (30) away from the rotating rod (32). The output end of the motor (31) is fixedly connected to the rotating rod (32). One end of each hinge rod (33) is hinged to the bottom end of a second fixing seat (27). The other end of each hinge rod (33) is hinged to one end of the rotating rod (32).

9. The cell culture device according to claim 8, characterized in that, Avoidance grooves (53) are formed in the middle of the bottom ends of the first door panel (14) and the second door panel (16). A closing component is arranged on one side of each avoidance groove (53).

10. A cell culture device according to claim 9, characterized in that, Each closed component includes a telescopic plate (54), a baffle plate (55), two guide rods (56) and two second springs (57). The baffle plate (55) is installed on one side of the first door panel (14). The telescopic plate (54) is located on one side of the avoidance groove (53) and is in contact with the first door panel (14). The two guide rods (56) are symmetrically and slidably arranged on the baffle plate (55). The bottom end of each guide rod (56) is fixedly connected to the telescopic plate (54). Each second spring (57) is sleeved on a guide rod (56). The two ends of each second spring (57) are respectively connected to the baffle plate (55) and the telescopic plate (54).

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