Oral composition

Abstract

The oral composition contains a specific sugar, an amino acid, and a citric acid. The specific sugar is a polysaccharide comprising, as structural sugars, a single galactose and a single or a plurality of other sugars other than galactose. The citric acid is selected from at least one of citric acid and citrate. The content of the specific sugar is 0.5-5% by mass (inclusive), the content of the amino acid is 0.5-4% by mass (inclusive), and the content of the citric acid is 0.001-2% by mass (inclusive).

Description

Technical Field

[0001] The present disclosure relates to oral compositions. Background Art

[0002] As a kind of non-pathogenic bacteria in the bacteria in the oral cavity, oral streptococcus (Streptococcusoralis) (hereinafter also referred to as oral streptococcus (S.oralis)) is known. Oral streptococcus belongs to the mitis group (mitis group) of the genus Streptococcus (Streptococcus). Other bacterial species belonging to the mitis group are also classified as non-pathogenic bacteria like oral streptococcus.

[0003] Patent Document 1 discloses an oral composition that selectively inhibits the growth of pathogenic bacteria without affecting the growth of Streptococcus mitis species belonging to the genus Streptococcus. In other words, the oral composition exhibits a selective antibacterial effect against pathogenic bacteria in the oral cavity. Improving the balance of oral bacterial flora by increasing the proportion of non-pathogenic bacteria in the oral bacterial flora is useful for preventing and inhibiting the progression of oral diseases. Prior art literature Patent Literature

[0004] Patent Document 1: Japanese Patent Application Laid-Open No. 2016-113460 Summary of the Invention Problems to be solved by the invention

[0005] From the perspective of ensuring a healthy oral environment, it has been suggested that increasing the concentration of oral streptococci in the oral cavity is useful. The present inventors have discovered that a combination of specific sugars and amino acids contributes to the proliferation of oral streptococci. However, when sugars and amino acids are used in combination, a Maillard reaction occurs, in which the sugars and amino acids react to produce a brown substance. Therefore, compositions mixed with sugars and amino acids are prone to discoloration caused by the Maillard reaction. Means for solving problems

[0006] A method of recording the present disclosure. [Method 1] An oral composition comprising a specific sugar, an amino acid, and a citric acid, wherein the specific sugar is a polysaccharide containing a single galactose as a structural sugar, and the citric acid is at least one selected from citric acid and a citrate, wherein the content of the specific sugar is from 0.5% to 5% by mass, the content of the amino acid is from 0.5% to 4% by mass, and the content of the citric acid is from 0.001% to 2% by mass.

[0007] [Method 2] The oral composition according to [Embodiment 1], wherein the specific sugar is a non-reducing sugar. [Method 3] The oral composition according to [Embodiment 2], wherein the amino acid is at least one selected from basic amino acids and acidic amino acids, the basic amino acid is at least one selected from basic amino acids and basic amino acid salts, and the acidic amino acid is at least one selected from acidic amino acids and acidic amino acid salts.

[0008] [Method 4] The oral composition according to [Embodiment 3], wherein the basic amino acid is arginine, the acidic amino acid is at least one selected from glutamic acid, glutamate, aspartic acid, and aspartate, and the non-reducing sugar is raffinose.

[0009] [Method 5] The oral composition according to [Embodiment 2], wherein the amino acid is a neutral amino acid. [Method 6] The oral composition according to [Embodiment 5], wherein the neutral amino acid is citrulline, and the non-reducing sugar is raffinose.

[0010] [Method 7] The oral composition according to [Embodiment 1], wherein the specific sugar is lactose, and the amino acid is at least one acidic amino acid selected from the group consisting of acidic amino acids and acidic amino acid salts.

[0011] [Method 8] The oral composition according to [Embodiment 7], wherein the acidic amino acid is at least one selected from glutamic acid, glutamate salts, aspartic acid, and aspartate salts.

[0012] [Method 9] The oral composition according to any one of [Aspect 1] to [Aspect 7], which is used as an oral composition for bacteria proliferation that promotes the growth of oral Streptococcus. DETAILED DESCRIPTION

[0013] Hereinafter, the oral composition according to this embodiment will be described in detail. The oral composition of the present embodiment is used as, for example, an oral composition for bacteria proliferation for the purpose of improving the balance of bacterial flora in the oral cavity, specifically, for the purpose of promoting the proliferation of oral Streptococcus.

[0014] The oral composition of this embodiment contains specific sugars, amino acids, and citric acids. The pH of the oral composition is, for example, 5.5 to 9.0, or less, preferably 6.0 to 8.5.

[0015] (Specific sugars) Specific sugars are polysaccharides containing a single galactose as a structural sugar. In other words, they are heteropolysaccharides containing a single galactose and a single or multiple other sugars other than galactose as structural sugars. In specific sugars, the structural sugars are linked to each other via glycosidic bonds. The number of monosaccharide bonds in a specific sugar is, for example, 2 or more and 5 or less, preferably 2 or more and 4 or less. Specific sugars are, for example, disaccharides and trisaccharides. Specific sugars may be oligosaccharides having 4 to 9 monosaccharide bonds, or polysaccharides having 10 or more monosaccharide bonds.

[0016] Examples of the structural sugars other than galactose contained in the specific sugar include hexose sugars such as glucose, fructose, and fucose, and pentose sugars such as xylose and ribose. The structural sugars other than galactose are preferably hexose sugars.

[0017] Examples of specific sugars include reducing sugars such as lactose and non-reducing sugars such as raffinose. Lactose is a disaccharide composed of one galactose and one glucose as structural sugars. Raffinose is a trisaccharide composed of one fructose, one galactose, and one glucose as structural sugars. The oral composition may contain only one of the above specific sugars, or a combination of two or more.

[0018] It should be noted that reducing sugars refer to sugars having free aldehyde or ketone groups or aldehyde or ketone groups bonded via hemiacetal. Non-reducing sugars refer to sugars that do not have free reducing groups compared to reducing sugars. In other words, non-reducing sugars refer to sugars that have neither free aldehyde or ketone groups nor aldehyde or ketone groups bonded via hemiacetal.

[0019] The content of the specific sugar in the oral composition is 0.5% by mass or more and 5% by mass or less. The content of the specific sugar is preferably 1% by mass or more, more preferably 1.5% by mass or more. The content of the specific sugar is preferably 4% by mass or less, more preferably 3% by mass or less.

[0020] In addition, the oral composition may contain other sugars that do not belong to the specific sugars. In this case, the content of the other sugars in the oral composition is preferably 5% by mass or less, for example.

[0021] (amino acids) The amino acid contained in the oral composition is not particularly limited. As the above-mentioned amino acid, for example, basic amino acids such as lysine, arginine, histidine and basic amino acid salts (hereinafter collectively recorded as basic amino acids), acidic amino acids such as aspartic acid, glutamic acid and acidic amino acid salts (hereinafter collectively recorded as acidic amino acids), neutral amino acids such as alanine, proline, threonine, serine, valine, glycine, citrulline, etc. can be enumerated. As a specific example of the basic amino acid salt, hydrochloride and sulfate can be enumerated. As a specific example of the acidic amino acid salt, alkali metal salts such as sodium salt, potassium salt, alkaline earth metal salts such as magnesium salt, calcium salt can be enumerated. The oral composition can contain only one of the above-mentioned amino acids alone, or can contain two or more in combination.

[0022] The content of amino acids in the oral composition is 0.5% by mass or more and 4% by mass or less. The content of amino acids is preferably 1% by mass or more, more preferably 1.5% by mass or more. The content of amino acids is preferably 3.5% by mass or less, more preferably 3% by mass or less. In addition, in the oral composition, the mass ratio of amino acids to specific sugars (amino acids / specific sugars) is, for example, 0.05 or more and 10 or less, preferably 0.25 or more and 4 or less.

[0023] (combination of specific sugars and amino acids) Preferred combinations of specific sugars and amino acids include combinations of non-reducing sugars and amino acids. In this case, the type of amino acid is not particularly limited and may be any of basic amino acids, acidic amino acids, and neutral amino acids. Specific examples of combinations of non-reducing sugars and amino acids include combinations of raffinose and arginine, combinations of raffinose and glutamic acid, combinations of raffinose and glutamate, combinations of raffinose and aspartic acid, combinations of raffinose and aspartate, and combinations of raffinose and citrulline.

[0024] In addition, as another preferred combination of specific sugars and amino acids, a combination of lactose and acidic amino acids can be mentioned. Specific examples thereof include a combination of lactose and glutamic acid, a combination of lactose and glutamate, a combination of lactose and aspartic acid, and a combination of lactose and aspartate.

[0025] (Citric Acid) The citric acid compound contained in the oral composition is at least one selected from citric acid and citrates. Examples of citrates include sodium citrate, magnesium citrate, potassium citrate, and calcium citrate.

[0026] The content of citric acid in the oral composition is 0.001% by mass or more and 2% by mass or less. The citric acid content is preferably 0.003% by mass or more, more preferably 0.005% by mass or more. The citric acid content is preferably 1.8% by mass or less, more preferably 1.5% by mass or less.

[0027] When the amino acid contained in the oral composition is a basic amino acid, the citric acid is preferably citric acid. In this case, the mass ratio of citric acid to basic amino acids in the oral composition (citric acid / basic amino acids) is, for example, 0.001 to 1.5, preferably 0.005 to 1.

[0028] When the amino acids contained in the oral composition are acidic amino acids, the mass ratio of citric acid to acidic amino acids in the oral composition (citric acid / acidic amino acids) is, for example, 0.001 to 1, preferably 0.005 to 0.8.

[0029] When the amino acids contained in the oral composition are neutral amino acids, the mass ratio of citric acid to neutral amino acids (citric acid / neutral amino acids) in the oral composition is, for example, 0.001 to 1, preferably 0.005 to 0.8.

[0030] (Application, use and dosage form) The application mode of the oral composition is not particularly limited, and for example, it can be used as a pharmaceutical or quasi-pharmaceutical. As the use of the oral composition, known uses can be appropriately adopted, for example, toothpaste, mouthwash, gargle, liquid dentifrice, biofilm dispersant, bad breath preventive, gum massage agent, oral moisturizing agent, tongue coating remover, oral coating, oral disinfectant, throat disinfectant, oral and throat agent, periodontal disease therapeutic agent, denture installation agent, denture coating agent, denture stabilizer, denture preservative, denture cleaning agent, and implant care agent.

[0031] The dosage form of the oral composition is not particularly limited, and by containing a solvent such as water or alcohol, the composition can be applied to an ointment, paste, cream, spray, gel, liquid, suspoemulsifier, or oral gum.

[0032] The type of water used as a solvent is not particularly limited, and for example, distilled water, pure water, ultrapure water, purified water, tap water, etc. can be used. The type of alcohol used as a solvent is not particularly limited, and for example, ethanol can be used. Water and alcohol can also be mixed and used. When the oral composition is in a liquid form, the content of the solvent such as water is preferably 60 to 99.8% by mass, more preferably 70 to 95% by mass.

[0033] (Other ingredients) The oral composition may contain other ingredients in addition to the above-mentioned ingredients according to the application purpose, form, use, etc. As other ingredients, for example, antibacterial agents, anti-inflammatory agents, fragrances, wetting agents, surfactants, abrasives, alcohols, thickeners, sweetening ingredients, medicinal ingredients, colorants, and stabilizers can be mentioned. Other ingredients can use known ingredients mixed in oral compositions. In the oral composition, the above-mentioned other ingredients can be contained alone or in combination of two or more.

[0034] Examples of the antibacterial agent include cetylpyridinium chloride, parabens, sodium benzoate, triclosan, chlorhexidine hydrochloride, isopropylmethylphenol, benzalkonium chloride, benzethonium chloride, and hinokitiol.

[0035] Examples of the anti-inflammatory agent include allantoin, glycyrrhetinic acid, glycyrrhizic acid salts, tranexamic acid, ε-aminocaproic acid, and Phellodendron amurense extract. Examples of the flavoring component include menthol, anethole, eugenol, carvone, wintergreen, methyl salicylate, thymol, clove oil, sage oil, ocimene oil, and citronellol.

[0036] Examples of the surfactant include nonionic surfactants, anionic surfactants, and amphoteric surfactants. Examples of the nonionic surfactant include sugar fatty acid esters such as sucrose fatty acid ester and maltose fatty acid ester, sugar alcohol fatty acid esters such as maltitol fatty acid ester, sorbitan fatty acid esters such as sorbitan monolaurate, polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene sorbitan monolaurate and polyoxyethylene sorbitan monostearate, fatty acid alkanolamides such as lauric acid diethanolamide, polyoxyethylene stearyl ether and polyoxyethylene oleyl ether, polyethylene glycol fatty acid esters such as polyethylene glycol monooleate and polyethylene glycol monolaurate, alkyl glucosides such as lauryl glucoside and decyl glucoside, polyglycerol fatty acid ester, polyoxyethylene glycerol fatty acid ester, polyoxyethylene fatty acid esters, alkyl glucosides, polyoxyethylene hydrogenated castor oil, glycerol fatty acid ester, and polyoxyethylene propylene block copolymers.

[0037] Examples of the anionic surfactant include sulfate ester salts such as sodium lauryl sulfate and sodium polyoxyethylene lauryl ether sulfate, sulfosuccinates such as sodium lauryl sulfosuccinate and sodium polyoxyethylene lauryl ether sulfosuccinate, acylamino acid salts such as sodium cocoyl sarcosinate and sodium lauroyl methylalanine, and sodium cocoyl methyl taurate.

[0038] Examples of the amphoteric surfactant include amino acid-type amphoteric surfactants such as N-lauryldiaminoethylglycine and N-myristyldiethylglycine; and betaine-based amphoteric surfactants such as alkyldimethylaminoacetic acid betaine, N-alkyl-N'-carboxymethyl-N'-hydroxyethylethylenediamine salts, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine and cocamidopropyl betaine.

[0039] Examples of the abrasive include calcium carbonate, magnesium carbonate, calcium hydrogen phosphate, tricalcium phosphate, magnesium phosphate, silica, zeolite, sodium metaphosphate, aluminum hydroxide, magnesium hydroxide, calcium pyrophosphate, red iron oxide, calcium sulfate, and silicic anhydride.

[0040] Examples of the alcohols include ethanol, lauryl alcohol, and myristyl alcohol. Examples of the thickener include sodium polyacrylate, carrageenan, sodium carboxymethylcellulose, sodium alginate, xanthan gum, hydroxyethylcellulose, hydroxypropylmethylcellulose, methylcellulose, and propylene glycol alginate.

[0041] Examples of the pharmaceutical ingredients include fluorides such as sodium monofluorophosphate, sodium fluoride, stannous fluoride, and strontium fluoride; condensed phosphates such as sodium pyrophosphate and sodium polyphosphate; phosphates such as sodium dihydrogen phosphate and trisodium phosphate; vitamins such as ascorbic acid, sodium ascorbate, pyridoxine hydrochloride, and tocopherol acetate; dextranases such as dextranase and mutanase; degrading enzymes such as protease and lysozyme; inorganic salts such as zinc chloride, zinc citrate, strontium chloride, potassium nitrate, and aluminum lactate; chelating compounds such as chlorophyll and glycerophosphate; polyethylene glycol for solubilizing lipids; sodium chloride, aluminum lactate, and strontium chloride.

[0042] Examples of colorants include legal pigments such as Green No. 1, Blue No. 1, and Yellow No. 4, as well as titanium oxide. It should be noted that when the oral composition is transparent or translucent, particularly in a liquid form, discoloration caused by the brown substance generated by the Maillard reaction is easily noticeable. Therefore, the discoloration-suppressing effect of this embodiment can be particularly significant when the oral composition is in a transparent or translucent liquid form.

[0043] Examples of the stabilizer include sodium edetate, sodium thiosulfate, sodium sulfite, calcium lactate, lanolin, triacetin, castor oil, and magnesium sulfate.

[0044] (Effect) Next, the operation and effects of this embodiment will be described. (1) An oral composition contains a specific sugar, an amino acid, and a citric acid. The specific sugar is a polysaccharide containing a single galactose as a structural sugar. The citric acid is at least one selected from citric acid and a citrate. The content of the specific sugar is 0.5% to 5% by mass, the content of the amino acid is 0.5% to 4% by mass, and the content of the citric acid is 0.001% to 2% by mass.

[0045] According to the above configuration, oral Streptococcus can be proliferated in the oral cavity and discoloration of the oral composition can be suppressed. (2) The specific sugar is a non-reducing sugar. According to the above-mentioned structure, the discoloration suppression effect described in (1) above can be more significantly obtained. In particular, in this case, the Maillard reaction in which the specific sugar reacts with the amino acid to generate a brown substance can be effectively suppressed, thereby further effectively suppressing the discoloration of the oral composition. In addition, the specific sugar and the amino acid are not easily consumed by the Maillard reaction, thereby suppressing the reduction of the concentrations of the specific sugar and the amino acid in the oral composition. Therefore, from the two aspects of suppressing discoloration and maintaining the proliferation-promoting effect of oral streptococci, the oral composition of the above-mentioned structure can be stored stably for a long time. In addition, the non-reducing sugar is particularly preferably raffinose.

[0046] (3) In the composition of (2) above, the amino acid is at least one selected from basic amino acids and acidic amino acids. The basic amino acid is at least one selected from basic amino acids and basic amino acid salts. The acidic amino acid is at least one selected from acidic amino acids and acidic amino acid salts.

[0047] According to the above configuration, the discoloration suppression effect described in (2) above can be more significantly obtained. It should be noted that the basic amino acid is particularly preferably arginine. The acidic amino acid is particularly preferably at least one selected from glutamic acid, glutamate, aspartic acid, and aspartate.

[0048] (4) In the structure of (2) above, the amino acid is a neutral amino acid. According to the above structure, the discoloration suppressing effect described in (2) above can be more significantly obtained. The neutral amino acid is particularly preferably citrulline.

[0049] (5) The specific sugar is lactose. The amino acid is at least one acidic amino acid selected from acidic amino acids and acidic amino acid salts. According to the above composition, the discoloration suppression effect described in (1) above can be more significantly obtained. It should be noted that the acidic amino acid is particularly preferably at least one selected from glutamic acid, glutamate, aspartic acid, and aspartate. Example

[0050] The oral composition of the present disclosure will be described in further detail based on the following examples and comparative examples. It should be noted that the oral composition of the present disclosure is not limited to the configurations of the following examples. (Preparation of Examples 1 to 15 and Comparative Examples 1 and 2) As Examples 1 to 15 and Comparative Examples 1 and 2, the components were mixed and stirred according to conventional methods to prepare liquid mouthwashes of the formulation examples shown in Table 1. The types and amounts of sugars, amino acids, and citric acids used in each example are shown in Tables 2 to 4. The values ​​of the components in the tables are expressed in mass %.

[0051] [Table 1] Element Compounding amount (mass %) sugar 1.5～5.0 amino acids 1.5 Citric acid 0.01～0.71 water margin total 100

[0052] (Long-term storage evaluation) Each of the prepared Examples and Comparative Examples was sealed and stored at 55°C for one month. The yellowness of each Example was measured before and after the above storage treatment, and the rate of change in yellowness (after treatment / before treatment) was determined. The yellowness was measured using a UV-visible spectrophotometer (UV-2600) manufactured by Shimadzu Corporation. The coloration suppression effect of each Example was then evaluated based on the rate of change in yellowness according to the following criteria. The results are shown in Tables 2 to 4.

[0053] A: The rate of change in yellowness is 2.0 or less, and the coloration suppression effect is high. B: The rate of change in yellowness exceeds 2.0 and is 4.0 or less, indicating a coloration-suppressing effect. C: The rate of change in yellowness exceeded 4.0, indicating no coloration suppressing effect.

[0054] Tables 2 to 4 show the pH of each example measured before the above-mentioned storage treatment.

[0055] [Table 2]

[0056] [Table 3]

[0057] [Table 4]

[0058] Comparative Example 1 contained galactose, a monosaccharide, as a sugar. Comparative Example 2 contained galacto-oligosaccharide, a trisaccharide composed of two galactoses and one glucose as structural sugars. As shown in Table 2, the change in yellowness before and after storage treatment in Comparative Examples 1 and 2 was 20 or more, indicating significant discoloration due to storage treatment.

[0059] On the other hand, Examples 1 to 10, 14, and 15 contained raffinose, a trisaccharide and non-reducing sugar, with one fructose, one galactose, and one glucose as structural sugars. Examples 11 to 13 contained lactose, a disaccharide and reducing sugar, with one galactose and one glucose as structural sugars. As shown in Tables 2 to 4, in Examples 1 to 15, the rate of change in yellowness before and after storage treatment was 4 or less, which significantly suppressed discoloration caused by storage treatment compared to Comparative Examples 1 and 2, where the rate of change was 20 or more. These results show that the use of a polysaccharide containing a single galactose as a structural sugar as a specific sugar can achieve a discoloration-suppressing effect.

[0060] In Examples 1 to 10, 14, and 15, in which the sugar contained a non-reducing sugar, the rate of change in yellowness before and after storage was 2 or less, indicating a particularly high effect in suppressing discoloration due to storage. Similarly, in Examples 12 and 13, in which the sugar and amino acid combination consisted of an acidic amino acid (monosodium glutamate or sodium aspartate) and a reducing sugar (lactose), the rate of change in yellowness before and after storage was also 2 or less, indicating a particularly high effect in suppressing discoloration due to storage.

[0061] Furthermore, the rate of change in yellowness varies depending on the content of citric acid, as shown in the results of Examples 1 to 5 and Examples 14 and 15. This result shows that the presence of citric acid is important for achieving the discoloration suppression effect of the Examples.

[0062] (Culture test of oral streptococci (S.oralis)) Oral Streptococcus was cultured in BHI medium (Brain Heart Infusion broth, manufactured by BD Biosciences). The turbidity of the culture solution was measured at a wavelength of 660 nm, and then the culture solution was diluted with BHI medium to a turbidity of 0.1. This was used as the bacterial solution. Streptococcus oralis spp. tigurinus was used as the oral Streptococcus.

[0063] Sample solutions corresponding to Examples 1, 9 to 13, 14, and 15 were prepared. The sample solutions differed from those of Examples 1, 9 to 13, 14, and 15 only in that the water having the composition shown in Table 1 was replaced with BHI medium.

[0064] 100 μl of bacterial suspension and 100 μl of sample solution were added to the wells of a 96-well plate and cultured at 37°C under anaerobic conditions for 24 hours. After culture, the supernatant was removed, and 110 μl of Alamar Blue solution (Invitrogen) diluted 11-fold with phosphate-buffered saline (PBS) was added. Fluorescence intensity was measured under the conditions of an excitation wavelength of 545 nm and a fluorescence wavelength of 590 nm.

[0065] The relative value of the fluorescence intensity relative to the control without sample solution was calculated and used as the growth rate of oral Streptococcus (S.oralis). As a result, when any of the sample solutions corresponding to Examples 1, 9 to 13, 14, and 15 were used, the growth rate exceeded 1, confirming the effect of promoting the growth of oral Streptococcus.

Claims

1. An oral composition, wherein: The composition contains specific sugars, amino acids and citric acids. The specific sugar is a polysaccharide containing a single galactose as a structural sugar, The citric acid is at least one selected from citric acid and citrate, The content of the specific sugar is 0.5% by mass or more and 5% by mass or less, The content of the amino acid is 0.5% by mass or more and 4% by mass or less, The content of the citric acid is 0.001% by mass or more and 2% by mass or less.

2. The oral composition according to claim 1, wherein The specific sugar is a non-reducing sugar.

3. The oral composition according to claim 2, wherein The amino acid is at least one selected from basic amino acids and acidic amino acids, The basic amino acid is at least one selected from basic amino acids and basic amino acid salts. The acidic amino acid is at least one selected from acidic amino acids and acidic amino acid salts.

4. The oral composition according to claim 3, wherein The basic amino acid is arginine, The acidic amino acid is at least one selected from glutamic acid, glutamate, aspartic acid and aspartate, The non-reducing sugar is raffinose.

5. The oral composition according to claim 2, wherein The amino acids are neutral amino acids. The oral composition according to claim 5, wherein The neutral amino acid is citrulline, The non-reducing sugar is raffinose.

7. The oral composition according to claim 1, wherein The specific sugar is lactose, The amino acid is at least one acidic amino acid selected from acidic amino acids and acidic amino acid salts.

8. The oral composition according to claim 7, wherein The acidic amino acids are at least one selected from glutamic acid, glutamate, aspartic acid and aspartate.

9. The oral composition according to any one of claims 1 to 8, wherein This composition is used as an oral composition for promoting the growth of oral streptococci.

Images