Soothing composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a soothing composition as well as a preparation method and application thereof. The composition is prepared from saccharomycetes fermentation lysate filtrate, peony root extract, spanishneedles herb extract, liquorice root extract, glycerol and tremella fuciformis extract. Small molecule peptides in the fermentation lysate and flavone, polyphenol and triterpenoid components in the plant extract can inhibit inflammation pathways (such as TNF-alpha, IL-6 and COX-2) in a multi-target mode, and skin redness is effectively relieved. The skin barrier repairing capability is high: the tremella acidic polysaccharide and the yeast polypeptide form a synergistic network, so that the water content of a cuticle is favorably enhanced, and barrier lipid synthesis is promoted; a polysaccharide-polypeptide cross-linked chain structure is broken through a high-shear homogenizing treatment technology, so that the wiredrawing and colloid decorous feeling of a finished product are remarkably reduced, and a refreshing skin feeling is obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and in particular to a soothing composition and a preparation method and application thereof. Background Art

[0002] Impaired skin barrier function, frequent allergic reactions, and a persistently activated inflammatory microenvironment are pressing challenges in the current skincare industry. Particularly in modern urban environments, skin is more susceptible to dryness, itching, redness, and stinging due to irritants such as UV exposure, excessive cleansing, and cosmetic procedures. Consequently, market demand for skincare products with soothing, anti-inflammatory, and repairing properties has increased significantly.

[0003] Existing technologies include single-source plant extracts such as licorice, calendula, and Centella asiatica, which are widely used in soothing cosmetics. However, these single ingredients often have limited ability to regulate complex skin inflammatory factors. Furthermore, some natural active ingredients (such as polysaccharides, flavonoids, and fermentation products) can exhibit stickiness, stringiness, poor transparency, and unstable compatibility in formulations, impacting the skin feel and storage stability of the final product.

[0004] At the same time, the combination of natural plant extracts and yeast fermentation products has been preliminarily explored in the field of skin soothing, but there is still a lack of systematic synergistic optimization strategies. Especially under the coexistence conditions of polysaccharides, peptides and flavonoids, how to achieve stable compatibility, refreshing skin feel and significant efficacy is still a pain point in the industry.

[0005] Therefore, there is an urgent need to develop a new soothing composition based on the synergistic construction of multi-source natural extracts, while optimizing the preparation process and colloidal structure control method, so as to increase the application breadth and market acceptance of such natural formulas. Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a soothing composition, which comprises: yeast fermentation lysate filtrate, peony (PAEONIA SUFFRUTICOSA) root extract, three-leaf Bidens pilosa (BIDENSPILOSA) extract, licorice (GLYCYRRHIZAURALENSIS) extract, glycerin, and Tremella fuciformis (TREMELLA AFUCIFORMIS) extract.

[0007] As an embodiment of the present invention, the content of glycerol is 6 to 10 wt%.

[0008] As an embodiment of the present invention, the preparation method of the Bidens pilosa L. extract is as follows:

[0009] The aboveground part of the dried Bidens pilosa was crushed into 40 mesh, and 0.5 wt% β-glucosidase (dissolved in citric acid-sodium citrate buffer with a pH of 5.5, with a solid-liquid ratio of 1:10) was added and reacted at 37°C for 1 h;

[0010] Add 50% ethanol, extract at 60°C for 45 min at a solid-liquid ratio of 1:12, repeat the operation twice, combine the filtrates, and concentrate to 1 / 5 of the volume under low-temperature vacuum;

[0011] Using D101 macroporous resin, the sample was loaded at a volume ratio of 1:10, and the active components were eluted using water, 30% ethanol, and 70% ethanol respectively; the eluate was then collected, concentrated and stored, the pH was adjusted to 5.5, and stored at low temperature.

[0012] As an embodiment of the present invention, the extraction method of the licorice extract is:

[0013] Grind the licorice slices to 60 mesh size and extract them with 0.5% sodium hydroxide aqueous solution at a solid-liquid ratio of 1:10 at 70°C for 2 hours. Then filter the supernatant and adjust the pH to 5.5 with dilute hydrochloric acid.

[0014] Glycosidase (0.2% w / v) was added, and the mixture was reacted at 37°C for 2 h. After that, anhydrous ethanol was added to a final concentration of 70%, and the mixture was allowed to stand for 8 h, and the supernatant was collected.

[0015] Subsequently, the solution was eluted with a D101 resin column using ethanol of different concentration gradients, with the ethanol concentration ranging from 50% to 70% in the eluent segment; the pH was then adjusted to 6.0, the solution was sterilized by filtration with a 0.22 μm filter membrane, and stored at low temperature.

[0016] As an embodiment of the present invention, the composition further comprises p-hydroxyacetophenone and 1,2-hexanediol.

[0017] As an embodiment of the present invention, the content of 1,2-hexanediol is 0.8 to 1.2 wt%.

[0018] As an embodiment of the present invention, the composition further comprises HA with a molecular weight of kDa to 50 kDa.

[0019] As an embodiment of the present invention, the preparation method of the soothing composition comprises the following steps:

[0020] (1) Add the Tremella fuciformis extract to glycerol and stir evenly; let it stand at room temperature for 15 to 30 minutes for prehydration, and keep stirring at a low speed to avoid agglomeration;

[0021] (2) adding peony root extract, Bidens pilosa extract, and licorice extract in sequence; slowly stirring and mixing to form a clear or slightly yellow liquid;

[0022] (3) Slowly adding the yeast fermentation lysate filtrate; placing the above mixture into a homogenization reaction vessel;

[0023] (4) Use a high shear homogenizer at 5000-8000 rpm for 3-5 minutes; add HA and stir evenly;

[0024] (5) Add p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and adjust the pH to 5.5±0.2.

[0025] As an embodiment of the present invention, the soothing composition is used in the field of daily chemicals.

[0026] By adopting the above technical solution, the present invention has the following beneficial effects:

[0027] The present invention provides a soothing skin care composition composed of a yeast fermentation lysate filtrate, peony root extract, Bidens pilosa extract, licorice extract, Tremella fuciformis extract, and other composite ingredients. Combining a multi-phase natural active mechanism with high shear homogenization technology, it has the following beneficial effects:

[0028] 1. Multi-pathway synergistic soothing: Small molecule peptides in fermentation lysate, flavonoids, polyphenols, and triterpenes in plant extracts can inhibit inflammatory pathways (such as TNF-α, IL-6, COX-2, etc.) at multiple targets, effectively alleviating skin redness, stinging, and other discomfort symptoms;

[0029] 2. Strong skin barrier repair ability: Tremella acidic polysaccharides and yeast peptides form a synergistic network, which helps to increase the water content of the stratum corneum and promote the synthesis of barrier lipids;

[0030] 3. Light and non-sticky skin feel: Through high shear homogenization technology, the polysaccharide-polypeptide cross-linking chain structure is interrupted, significantly reducing the stringy and colloid-heavy feeling of the finished product, resulting in a refreshing skin feel;

[0031] 4. Good compatibility stability: The use of pH control, glycerol dispersion, staged addition and anti-corrosion synergistic strategies effectively solves problems such as precipitation, flocculation, and color change among plant ingredients, and improves the storage stability and sensory properties of the product. DETAILED DESCRIPTION

[0032] The present invention will be further explained below with reference to specific embodiments.

[0033] The setting instructions of the embodiment are as shown in Table 1.

[0034] Table 1 shows the specific formula of each embodiment

[0035]

[0036] In the above table, the preparation steps of the BIDENSPILOSA extract are as follows:

[0037] Mix the crushed dry aerial parts of Bidens pilosa with deionized water in a ratio of 1:10 (w / v), and heat directly to 100°C in an open beaker; continue heating at a boiling state for 40 minutes, and then filter.

[0038] The preparation steps of Glycyrrhiza uralensis extract are as follows:

[0039] Take the crushed dry licorice slices and put them into room temperature water with a solid-liquid ratio of 1:8; the extraction temperature is controlled at around 70°C and the extraction time is 30 minutes; then filter.

[0040] The preparation methods of the compositions in Examples 1 to 5 are as follows:

[0041] (1) Add the Tremella fuciformis extract to glycerol and stir evenly; let it stand at room temperature for 15 to 30 minutes for prehydration, and keep stirring at a low speed to avoid agglomeration;

[0042] (2) adding peony root extract, Bidens pilosa extract, and licorice extract in sequence; slowly stirring and mixing to form a clear or slightly yellow liquid;

[0043] (3) Slowly adding the yeast fermentation lysate filtrate; placing the above mixture into a homogenization reaction vessel;

[0044] (4) Use a high shear homogenizer at 5000-8000 rpm for 3-5 minutes; add HA and stir evenly (if any);

[0045] (5) Add p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and adjust the pH to 5.5±0.2.

[0046] The present invention also provides Example 6, which has the same formula as Example 1, but is prepared as follows:

[0047] (1) Add Tremella fuciformis extract, peony extract, Bidens pilosa extract, licorice extract and HA powder directly into distilled water and stir;

[0048] (2) The turbid system is directly treated with an 8000 rpm high shear homogenizer for 5 minutes; after high shearing, yeast fermentation filtrate is added to obtain p-hydroxyacetophenone and 1,2-hexanediol.

[0049] Performance testing:

[0050] Test 1: Zebrafish soothing effect (total movement distance) test method

[0051] 1. Experimental Principle

[0052] As an in vitro model system, the zebrafish (Daniorerio) is commonly used in studies of skin inflammation, neuropathic pain, and other conditions, due to its highly similar skin and nervous systems to humans. Exogenous stimuli (such as glacial acetic acid) can be used to induce a "pain / irritation"-like state in zebrafish, manifested by a significant increase in locomotor activity, or "total distance traveled." If the test sample possesses soothing or analgesic activity, the distance traveled by the zebrafish can be reduced, thereby measuring its soothing ability.

[0053] 2. Experimental Materials and Conditions

[0054]

[0055]

[0056] 3. Experimental Design

[0057] Grouping illustrate Normal control group Untreated zebrafish, incubated in feeding medium only Model control group Glacial acetic acid stimulation, no sample added Sample Group Glacial acetic acid stimulation + sample aqueous solution pretreatment

[0058] Three replicate wells were set up in each group, with 15 zebrafish per well, for a total sample size of n = 45 per group. Ten zebrafish were randomly selected from each group for behavioral testing.

[0059] IV. Experimental Procedure

[0060] Step 1: Zebrafish incubation and handling

[0061] Zebrafish embryos 2 days after fertilization were distributed into 6-well plates, with 15 embryos per well. Sample aqueous solution (diluted to 0.1%) (or blank culture medium / control solution) was added, with a volume of 3 mL per well. A normal control group (no sample added, no modeling) and a model control group (no sample added, subsequent glacial acetic acid modeling) were set up. All sample and control groups were incubated in the dark at 28°C for 24 h.

[0062] Step 2: Pain model establishment and behavioral testing

[0063] Randomly transfer 10 zebrafish from each group to a 96-well plate (1 fish per well). Add glacial acetic acid solution to induce a skin pain stimulation model in zebrafish. Immediately activate the behavioral analyzer to record the movement trajectory of each fish for 3 to 5 minutes. Collect the total movement distance D (unit: cm or mm) of each fish and output the data. Soothing effect (%) = (D (模型对照组) -D (样品组) ) / (D (模型对照组) -D (正常对照组) *100%.

[0064] Table 2 Test results of test 1

[0065]

[0066] Test 2: The compositions prepared in Examples 1 to 6 were subjected to temperature stability tests at 5°C, 25°C, and 40°C for 30 days. The test results are shown in Table 3. Hot and cold cycle experiment: The compositions prepared in Examples 1 to 6 were placed in sealed containers to ensure that they are completely sealed to prevent interference from external humidity or gas. Place the sample in a thermostat set at 45°C for 12 hours. After taking out the sample, quickly transfer it to a low-temperature box at 5°C and keep it for 12 hours. After completing one high and low temperature cycle, repeat 15 times; check the external properties of the sample after the cycle.

[0067] Table 3 Test results of performance test 2

[0068]

[0069]

[0070] Test 3: User experience test

[0071] This experiment aims to observe the soothing and usage effects of the compositions of Examples 1 to 6 as soothing cosmetics. The test period is 14 days. The test adopts a randomized single-blind method, and the subjects are self-controlled before and after the test.

[0072] Do not use any other skin care products during the trial period. Apply about 1g of the product on your face after washing your face every morning and evening, and record your experience.

[0073] Storage conditions: Avoid direct sunlight or high temperatures, store in a cool and dry place.

[0074] A total of 25 subjects evaluated the effects of the test products on D14. The evaluation results are shown in the table below: Scoring criteria (5-point system): Those with a statistical score of ≥3 points are considered to be satisfied.

[0075] Satisfaction = (number of valid people who chose satisfaction ÷ number of valid test participants) × 100%.

[0076] 1 is strongly disagree, 2 is disagree, 3 is uncertain, 4 is moderately agree, and 5 is strongly agree. To save space, the table only records satisfaction.

[0077] Table 4 Test results of performance test 3 of embodiment 1

[0078]

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A soothing composition, characterized in that The composition comprises: yeast fermentation lysate filtrate, peony root extract, Bidens pilosa extract, liquorice extract, glycerin and Tremella fuciformis extract.

2. The soothing composition according to claim 1, characterized in that The content of the glycerol is 6-10 wt%.

3. The soothing composition according to claim 1, characterized in that The preparation method of the Bidens pilosa L. extract is as follows: The aboveground part of the dried Bidens pilosa was crushed into 40 mesh, and 0.5 wt% β-glucosidase was added and reacted at 37°C for 1 h; Add 50% ethanol, extract at 60°C for 45 min at a solid-liquid ratio of 1:12, repeat the operation twice, combine the filtrates, and concentrate to 1 / 5 of the volume under low-temperature vacuum; D101 macroporous resin was used, and the sample was loaded at a volume ratio of 1:

10. The active components were eluted with water, 30% ethanol, and 70% ethanol, respectively. The eluate was then collected, concentrated, and stored, the pH was adjusted to 5.5, and stored at low temperature.

4. The soothing composition according to claim 1, characterized in that The extraction method of the licorice extract is: Grind the licorice slices to 60 mesh size and extract them with 0.5% sodium hydroxide aqueous solution at a solid-liquid ratio of 1:10 at 70°C for 2 hours. Then filter the supernatant and adjust the pH to 5.5 with dilute hydrochloric acid. Add glycosidase, react at 37°C for 2 h, then add anhydrous ethanol to a final concentration of 70%, let stand for 8 h, and collect the supernatant; Subsequently, the solution was eluted with a D101 resin column using ethanol of different concentration gradients, with the ethanol concentration ranging from 50% to 70% in the eluent segment; the pH was then adjusted to 6.0, the solution was sterilized by filtration with a 0.22 μm filter membrane, and stored at low temperature.

5. The soothing composition according to claim 1, characterized in that The composition also includes p-hydroxyacetophenone and 1,2-hexanediol.

6. The soothing composition according to claim 5, characterized in that The content of the 1,2-hexanediol is 0.8-1.2 wt%.

7. The soothing composition according to claim 1, characterized in that The composition also comprises HA with a molecular weight of 3 kDa to 50 kDa.

8. The method for preparing the soothing composition according to any one of claims 1 to 7, characterized in that: The preparation steps are as follows: (1) Add the Tremella fuciformis extract to glycerol and stir evenly; let it stand at room temperature for 15 to 30 minutes for prehydration, and keep stirring at a low speed to avoid agglomeration; (2) Add peony root extract, Bidens pilosa extract, and licorice extract in sequence; slowly stir and mix. Forms a clear or slightly yellow liquid; (3) Slowly adding the yeast fermentation lysate filtrate; placing the above mixture into a homogenization reaction vessel; Use a high shear homogenizer at 5000-8000 rpm for 3-5 minutes; add HA and stir evenly; (4) Add p-hydroxyacetophenone and 1,2-hexanediol, stir evenly, and adjust the pH to 5.5±0.

2.

9. The soothing composition according to any one of claims 1 to 7, characterized in that Used in daily chemical field.

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