Preparation method and application of water-soluble pogostemon cablin essential oil

Abstract

The invention discloses a preparation method and application of water-soluble pogostemon cablin essential oil, and the preparation method comprises the following steps: blending pogostemon cablin and ethanol, carrying out wet grinding treatment, freezing, drying and carrying out secondary grinding to obtain fine powder, then dissolving out and distilling the fine powder to obtain pogostemon cablin essential oil liquid, and carrying out spray drying on the pogostemon cablin essential oil liquid to obtain the water-soluble pogostemon cablin essential oil. And finally, preparing the water-soluble pogostemon cablin essential oil from the pogostemon cablin essential oil liquid, a surfactant and a cosurfactant. The invention further provides application of the water-soluble pogostemon cablin essential oil. According to the technical scheme, the formula is simple, procedures are simplified, and large-scale production and marketization popularization are facilitated; the stability of the pogostemon cablin essential oil is remarkably improved, and long-term storage and transportation are facilitated; active ingredients of the inner phase can be effectively protected, the slow release effect is achieved, the active ingredients stably play a role, and meanwhile absorption of the active ingredients is promoted; the plant essential oil is prepared into the water-soluble microemulsion, so that the water-soluble microemulsion can be suitable for more dosage forms, and the application range of the water-soluble microemulsion is greatly widened.

Description

Technical Field

[0001] The invention belongs to the technical field of essential oils, and particularly relates to a preparation method and application of water-soluble patchouli essential oil. Background Art

[0002] Patchouli, one of the "Top Ten Southern Medicinal Herbs," is pungent and slightly warm in nature, with aromatic properties for dehumidification, cough and dampness relief, and analgesia. Patchouli essential oil, a volatile oil extracted from the herb, is rich in active ingredients such as patchouliol, patchouli ketone, and patchoulene, and exhibits significant antibacterial, anti-allergic, and anti-inflammatory properties. Studies have shown that patchouli essential oil can effectively reduce TRPV1 expression in the skin, soothe skin conditions, alleviate skin discomfort, and contribute to peripheral analgesia. Patchouli essential oil is a cannabinoid receptor 2 agonist, activating cannabinoid receptor 2 without triggering cannabinoid receptor 1-mediated central nervous system side effects. It can inhibit the release of inflammatory mediators, thereby reducing inflammatory responses, and can also inhibit the transmission of pain signals, promote the repair of skin barrier function, and alleviate skin sensitivity. However, patchouli essential oil is inherently hydrophobic, making it difficult to dissolve in water, resulting in poor stability during application. Summary of the Invention

[0003] In response to the problems in the prior art, the present invention provides a method for preparing water-soluble patchouli essential oil, which solves the problem of poor water solubility of the existing patchouli essential oil. Patchouli is dispersed by a double refinement method, and an ethanol-water distillation system is used to form water-containing patchouli essential oil. The water-soluble patchouli essential oil is then combined with a surfactant, a co-surfactant, etc. to obtain a water-soluble system in which the essential oil is homogeneously dispersed.

[0004] In order to achieve the above technical objectives, the technical solution of the present invention is:

[0005] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0006] Step 1: Patchouli is blended with ethanol, wet-grinded for 1-4 hours, and subjected to reduced pressure distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 5-8:1, the temperature of the wet grinding is 10-15° C., the grinding pressure is 0.5-0.7 MPa, and the temperature of the reduced pressure distillation is 30-40° C., and the pressure is 90% of atmospheric pressure. This step utilizes the fluidity and stability of the liquid to ensure that the patchouli forms fine particles at room temperature without causing local temperature increase. During the reduced pressure distillation process, the volatility of ethanol is utilized to remove the ethanol without damaging the patchouli, thereby obtaining fine patchouli powder.

[0007] Step 2: allowing the patchouli particles to stand for 5-10 minutes, freeze-drying them, and then grinding them a second time for 1-2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 20-30° C.; the freeze-drying temperature is -20° C., and the grinding pressure is 0.6 MPa. This step utilizes the adsorption and permeability of humid air to form a deposit on the surface of the patchouli particles, thereby increasing the water content of the patchouli particles. The low temperature of freeze-drying can quickly convert the water in the particles into ice, causing the volume of the patchouli particles to expand, and the patchouli particles are secondary refined during grinding, thereby obtaining patchouli fine powder with a smaller particle size. Based on the further refinement of the patchouli particle size and the freeze expansion from the interior of the particles, the dissolution contact surface of the patchouli is increased, while the integrity of the patchouli is broken, which helps the solvent enter the interior, thereby improving the extraction efficiency and extraction yield.

[0008] Step 3: Place the patchouli powder in an ethanol-water solution and ultrasonicate it for 10-20 minutes, let it stand for 20-30 minutes, then seal and reflux it for 3-5 hours, and then cool it to room temperature to obtain a suspension. The volume ratio of ethanol in the ethanol-water solution is 10-20%. The mass ratio of patchouli powder to ethanol-water solution is 1:10-15, the ultrasonic temperature is 5-10°C, the ultrasonic frequency is 80-100kHz, and the standing temperature is 35-40°C; the sealed reflux temperature is 80-90°C, and steam is introduced to the bottom of the solution after reflux; this step utilizes the permeability and film-forming properties of the ethanol-water solution, combined with the high-frequency vibration and temperature control of the ultrasound, to infiltrate ethanol and water into the patchouli powder, thereby achieving a good dissolution effect; the temperature is slightly increased during the standing process, thereby improving the fluidity of the ethanol-water solution, effectively improving the flow effect of the dissolved matter, and helping to improve the dissolution effect, thereby achieving the effect of releasing essential oil molecules;

[0009] Step 4, distilling the suspension for 3-4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 103-105° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 80-90° C., and the temperature of the second gradient condensation is 10-15° C.; in this step, the essential oil, ethanol, and part of the water in the solution are converted into steam by distillation, and after the gradient treatment, the patchouli essential oil and ethanol mainly composed of water and patchouli essential oil are collected in sequence;

[0010] Step 5: adding a surfactant and a co-surfactant to a patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, adding water to the oil phase under high-speed stirring to prepare colostrum; injecting the prepared colostrum into a high-pressure microjet homogenizer; after homogenization, discharging and standing to obtain water-soluble patchouli essential oil; the premixing treatment adopts mechanical stirring, and the stirring speed is 300-500 r / min, and the temperature is 40-50° C.; the homogenization pressure of the high-pressure homogenizer is 40-60 MPa, the colostrum temperature is 35-40° C., and the number of homogenization times is 6-8 times; the mass fraction of the water-soluble patchouli essential oil is: 2-10% patchouli essential oil, 3-15% surfactant, 2-15% co-surfactant, and the balance is water. In this step, surfactants and co-surfactants are directly added to the aqueous patchouli essential oil solution. Premixing initially disperses the patchouli essential oil into the aqueous phase. High-speed stirring and a high-pressure homogenizer are then used to continuously disperse the patchouli essential oil droplets, ensuring the dispersibility of the patchouli essential oil droplets. This treatment method eliminates the need for dewatering the patchouli essential oil solution, effectively reducing the complexity of the patchouli essential oil process, improving its utilization rate, and minimizing process losses.

[0011] The surfactant is a composition of polyoxyethylene 40 hydrogenated castor oil and PPG-6-decyltetradecyl alcohol polyether-30. The composition has a good solubilizing effect on patchouli essential oil and improves the solubility and dispersibility of patchouli essential oil.

[0012] Wherein, the co-surfactant is one or more of n-propanol, 1,2-propylene glycol and 1,3-propylene glycol.

[0013] Furthermore, the mass ratio of the water-soluble patchouli essential oil is: 3-7% patchouli essential oil, 3-10% surfactant, 4-12% co-surfactant and the balance water.

[0014] Furthermore, the mass ratio of patchouli essential oil to surfactant is 1:1-3.

[0015] The mass ratio of surfactant to cosurfactant is 1:1-2.

[0016] Furthermore, the mass ratio of the patchouli essential oil, the surfactant and the co-surfactant is 1:1-3:1-3.

[0017] The water-soluble patchouli essential oil can be used to prepare a soothing and redness-reducing cream.

[0018] The mass ratio of the soothing and redness-removing cream is:

[0019] Phase A: C14-22 Alcohol / C12-20 Alkyl Glucoside: 0.5-1.5%; Glyceryl Stearate / PEG-100 Stearate: 0.2-0.5%; Caprylic / Capric Triglyceride: 0.5-1.0%; Shea Butter: 1.5-2%; Squalane: 1-2%; Dimethicone: 0.5%-0.8%;

[0020] Phase B: Acrylates / C10-30 Alkyl Acrylate Crosspolymer: 0.1-0.3%; Acrylates / C10-30 Alkyl Acrylate Crosspolymer: 0.1-0.2%; Hydrogenated Lecithin: 0.1-0.2%; Acetyl Glucosamine: 0.5-1%; Butylene Glycol: 1%-2%; Glycerin 6%-8%; Preservative: 0.5-1%;

[0021] Phase C: Arginine: 0.3-0.5%;

[0022] Phase D: Water-soluble patchouli essential oil: 2%-5%;

[0023] Phase E: Carnosine: 0.2-0.3%; Purified water: the balance.

[0024] Furthermore, the mass ratio of the soothing and redness-removing cream is as follows: Phase A: C14-22 alcohol / C12-20 alkyl glucoside: 0.5-0.8%; Glyceryl stearate / PEG-100 stearate: 0.2-0.3%; Caprylic / capric triglyceride: 0.5-1.0%; Shea butter: 1.5-2%; Squalane: 1-1.5%; Dimethicone: 0.5%-0.8%; Phase B: Acrylates / C10-30 alkyl propylene glycol Acid ester crosspolymer: 0.15-0.2%; Acrylates / C10-30 Alkyl Acrylate Crosspolymer: 0.15-0.2%; Hydrogenated Lecithin: 0.1-0.15%; Acetyl Glucosamine: 0.8-1%; Butylene Glycol: 1%-2%; Glycerin 7%-8%; Preservative: 0.6-1%; Phase C: Arginine: 0.3-0.4%; Phase D: Water-soluble Patchouli Essential Oil: 3%; Phase E: Carnosine: 0.2-0.3%; Purified Water: Balance.

[0025] The preservative is a composition of p-hydroxyacetophenone and hexylene glycol, and the mass ratio of p-hydroxyacetophenone to hexylene glycol is 1:1.

[0026] The preparation method of the soothing and redness-reducing cream comprises the following steps: a1, weighing phase A in a beaker, heating to 85°C and dissolving uniformly without particles; dissolving phases C and E separately in water for later use; a2, weighing phase B in another beaker, heating to 85°C and dissolving, keeping the temperature for 30 minutes, starting a homogenizer, and homogenizing for 3-5 minutes until a uniform state without particles; a3, pouring phase A into phase B, homogenizing for 3 minutes until a uniform state without particles; adding the dissolved phase C, stirring and cooling; a4, stirring and cooling to below 45°C, sequentially adding phases D and E, stirring and uniformly mixing, and discharging.

[0027] The water-soluble patchouli essential oil can be used to prepare a soothing and redness-reducing facial mask liquid.

[0028] The mass ratio of the soothing and redness-removing facial mask liquid is: Phase A: hydroxyethyl cellulose: 0.02-0.3%; Carbomer: 0.15-0.18%; Disodium EDTA: 0.05-0.7%; Dipotassium glycyrrhizate: 0.05-0.06%; Trehalose: 1-1.2%; Acetyl glucosamine: 0.5-0.8%; Allantoin: 0.2-0.4%; Glycerin: 7%-8%; Butylene glycol: 1%-1.5%; Preservative: 0.5-0.8%; Phase B: Arginine 0.2-0.3%; Phase C: Water-soluble Patchouli Essential Oil: 0.5%, and Purified Water: the remainder.

[0029] The preservative is a composition of p-hydroxyacetophenone and hexylene glycol, and the mass ratio of p-hydroxyacetophenone to hexylene glycol is 1:1.

[0030] The preparation method of the soothing and redness-removing facial mask liquid includes: weighing phase A, heating to 85°C to completely dissolve, and keeping warm for 30 minutes; dissolving phase B separately with water for later use; homogenizing phase A for 3-5 minutes until the material is uniform and free of particles, adding phase B, stirring and cooling to 45°C, weighing phase C, and stirring evenly; and discharging.

[0031] Furthermore, the soothing and redness-reducing facial mask liquid is filtered and sterilized, then put into the prepared facial mask paper, and sealed and packaged to obtain a 0.5% CB2 soothing and redness-reducing facial mask.

[0032] It can be seen from the above description that the present invention has the following advantages:

[0033] 1. The present invention solves the problem of poor water solubility of existing patchouli essential oil. Patchouli is dispersed by a double refinement method, and a distillation system of ethanol-water is used to form aqueous patchouli essential oil. The aqueous patchouli essential oil is then combined with a surfactant, a co-surfactant, etc. to obtain a water-soluble system in which the essential oil is homogeneously dispersed.

[0034] 2. The water-soluble patchouli essential oil prepared by the present invention has good water solubility, high in vitro effect stability, good safety, and can effectively provide the applicability of patchouli essential oil in skin care products.

[0035] 3. The soothing and redness-removing facial mask of the present invention has been tested for soothing efficacy in vitro and human efficacy, and can effectively inhibit the content of TRPV1 and increase the content of CB2, and has a good soothing and repairing effect.

[0036] 4. The patchouli moisturizing mask of the present invention has been tested on human body efficacy and can effectively improve skin redness and has the effect of instantly fading redness.

[0037] In summary, the patchouli essential oil prepared by this method has high extraction efficiency, effectively overcoming the water insolubility disadvantage of traditional patchouli essential oil, improving the stability of patchouli essential oil, facilitating long-term storage and transportation, and being applicable to a wider range of dosage forms, further enhancing the application of patchouli essential oil in cosmetic formulations. Creams and facial masks containing this water-soluble patchouli essential oil are gentle and non-irritating, suitable for sensitive skin, and can effectively reduce the degree of redness on sensitive skin, with a soothing and fading effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is a picture of the chicken embryo chorioallantoic membrane irritation experiment in Example 6.

[0039] Figure 2 This is a human efficacy test of the 3% CB2 soothing and redness-reducing cream in Example 7 on improving skin.

[0040] Figure 3 This is a human efficacy test of the 0.5% CB2 soothing and redness-reducing mask in Example 8 to improve skin. DETAILED DESCRIPTION

[0041] Combine Figures 1 to 3 , describes in detail the specific embodiments of the present invention, but does not limit the claims of the present invention.

[0042] Example 1

[0043] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0044] Step 1: blending patchouli with ethanol, wet-grinding the mixture for 1 hour, and performing vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 8:1, the wet-grinding temperature is 10° C., the grinding pressure is 0.5 MPa, and the vacuum distillation temperature is 30° C., and the pressure is 90% of atmospheric pressure;

[0045] Step 2: allowing the patchouli granules to stand for 5 minutes, freeze-drying them, and then grinding them again for 1 hour to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 20° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0046] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 10 minutes, allow to stand for 20 minutes, and then seal and reflux for 3 hours. After cooling to room temperature, a suspension is obtained. The volume ratio of ethanol in the ethanol-water solution is 20%. The mass ratio of patchouli powder to ethanol-water solution is 1:15. The ultrasonic temperature is 5°C, the ultrasonic frequency is 80kHz, and the standing temperature is 35°C. The sealed reflux temperature is 80°C, and steam is introduced to the bottom of the solution after reflux.

[0047] Step 4, distilling the suspension for 3 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 103° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 80° C. and the temperature of the second gradient condensation is 10° C.;

[0048] Step 5: adding a surfactant and a co-surfactant to a patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, adding water to the oil phase under high-speed stirring to prepare colostrum; injecting the prepared colostrum into a high-pressure microjet homogenizer; after homogenization, discharging and standing to obtain water-soluble patchouli essential oil; the premixing process adopts mechanical stirring, and the stirring speed is 300 r / min, and the temperature is 40°C; the homogenization pressure of the high-pressure homogenizer is 40 MPa, the colostrum temperature is 35°C, and the number of homogenization times is 6 times; the mass fraction of the water-soluble patchouli essential oil is: 10% patchouli essential oil, 15% surfactant, 2% co-surfactant, and the balance is water.

[0049] The surfactant is a compound of PPG-6-decyltetradecyl alcohol polyether-30 and polyoxyethylene 40 hydrogenated castor oil in a mass ratio of 1:2, and the co-surfactant is n-propanol.

[0050] Example 2

[0051] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0052] Step 1: patchouli and ethanol are blended and wet-grinded for 4 hours, followed by vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 5:1, the wet-grinding temperature is 15° C., the grinding pressure is 0.7 MPa, and the vacuum distillation temperature is 40° C., and the pressure is 90% of atmospheric pressure;

[0053] Step 2: allowing the patchouli particles to stand for 10 minutes, freeze-drying them, and then grinding them again for 2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 30° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0054] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 20 minutes, allow to stand for 30 minutes, then seal and reflux for 5 hours, cool to room temperature to obtain a suspension, wherein the volume ratio of ethanol in the ethanol-water solution is 10%. The mass ratio of patchouli powder to ethanol-water solution is 1:10, the ultrasonic temperature is 10°C, the ultrasonic frequency is 100kHz, and the standing temperature is 40°C; the sealed reflux temperature is 90°C, and steam is introduced to the bottom of the solution after reflux.

[0055] Step 4, distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 105° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 90° C. and the temperature of the second gradient condensation is 15° C.;

[0056] Step 5: adding a surfactant and a co-surfactant to a patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, adding water to the oil phase under high-speed stirring to prepare colostrum; injecting the prepared colostrum into a high-pressure microfluidizer; after homogenization, discharging and standing to obtain water-soluble patchouli essential oil; the premixing treatment adopts mechanical stirring, and the stirring speed is 500 r / min, and the temperature is 50° C.; the homogenization pressure of the high-pressure homogenizer is 60 MPa, the colostrum temperature is 40° C., and the number of homogenization times is 8 times; the mass fraction of the water-soluble patchouli essential oil is: 2% patchouli essential oil, 3% surfactant, 15% co-surfactant, and the balance is water.

[0057] The surfactant is a compound of PPG-6-decyltetradecyl alcohol polyether-30 and polyoxyethylene 40 hydrogenated castor oil in a mass ratio of 1:2, and the co-surfactant is 1,3-propylene glycol.

[0058] Example 3

[0059] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0060] Step 1: patchouli and ethanol are blended and wet-grinded for 3 hours, followed by vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 6:1, the wet-grinding temperature is 12° C., the grinding pressure is 0.6 MPa, and the vacuum distillation temperature is 35° C., and the pressure is 90% of atmospheric pressure;

[0061] Step 2: allowing the patchouli granules to stand for 8 minutes, freeze-drying them, and then grinding them again for 2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 25° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0062] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 15 minutes, allow to stand for 25 minutes, and then seal and reflux for 4 hours. After cooling to room temperature, a suspension is obtained. The volume ratio of ethanol in the ethanol-water solution is 15%. The mass ratio of patchouli powder to ethanol-water solution is 1:12. The ultrasonic temperature is 8°C, the ultrasonic frequency is 90kHz, and the standing temperature is 38°C. The sealed reflux temperature is 85°C, and steam is introduced to the bottom of the solution after reflux.

[0063] Step 4, distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 104° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 85° C. and the temperature of the second gradient condensation is 12° C.;

[0064] Step 5: adding a surfactant and a co-surfactant to a patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, adding water to the oil phase under high-speed stirring to prepare colostrum; injecting the prepared colostrum into a high-pressure microjet homogenizer; after homogenization, discharging and standing to obtain water-soluble patchouli essential oil; the premixing process adopts mechanical stirring, and the stirring speed is 400 r / min, and the temperature is 45° C.; the homogenization pressure of the high-pressure homogenizer is 50 MPa, the colostrum temperature is 38° C., and the number of homogenization times is 7 times; the mass fraction of the water-soluble patchouli essential oil is: 5% patchouli essential oil, 9% surfactant, 10% co-surfactant, and the balance water.

[0065] Among them, the surfactant is a compound of PPG-6-decyltetradecyl alcohol polyether-30 and polyoxyethylene 40 hydrogenated castor oil in a mass ratio of 1:1, and the co-surfactant is 1,3-propylene glycol.

[0066] Example 4

[0067] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0068] Step 1: patchouli and ethanol are blended and wet-grinded for 3 hours, followed by vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 6:1, the wet-grinding temperature is 12° C., the grinding pressure is 0.6 MPa, and the vacuum distillation temperature is 35° C., and the pressure is 90% of atmospheric pressure;

[0069] Step 2: allowing the patchouli granules to stand for 8 minutes, freeze-drying them, and then grinding them again for 2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 25° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0070] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 15 minutes, allow to stand for 25 minutes, and then seal and reflux for 4 hours. After cooling to room temperature, a suspension is obtained. The volume ratio of ethanol in the ethanol-water solution is 15%. The mass ratio of patchouli powder to ethanol-water solution is 1:12. The ultrasonic temperature is 8°C, the ultrasonic frequency is 90kHz, and the standing temperature is 38°C. The sealed reflux temperature is 85°C, and steam is introduced to the bottom of the solution after reflux.

[0071] Step 4, distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 104° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 85° C. and the temperature of the second gradient condensation is 12° C.;

[0072] Step 5: adding a surfactant and a co-surfactant to a patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, adding water to the oil phase under high-speed stirring to prepare colostrum; injecting the prepared colostrum into a high-pressure micro-jet homogenizer; after homogenization, discharging and standing to obtain water-soluble patchouli essential oil; the premixing treatment adopts mechanical stirring, and the stirring speed is 400 r / min, and the temperature is 45° C.; the homogenization pressure of the high-pressure homogenizer is 50 MPa, the colostrum temperature is 38° C., and the number of homogenization times is 7 times. The mass fraction of the water-soluble patchouli essential oil is: 5% patchouli essential oil, 8% surfactant, 12% co-surfactant, and the balance water.

[0073] Among them, the surfactant is a compound of PPG-PPG-6-decyltetradecyl alcohol polyether-30 and polyoxyethylene 40 hydrogenated castor oil in a mass ratio of 1:2, and the co-surfactant is 1,3-propylene glycol.

[0074] Example 5

[0075] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0076] Step 1: patchouli and ethanol are blended and wet-grinded for 3 hours, followed by vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 6:1, the wet-grinding temperature is 12° C., the grinding pressure is 0.6 MPa, and the vacuum distillation temperature is 35° C., and the pressure is 90% of atmospheric pressure;

[0077] Step 2: allowing the patchouli granules to stand for 8 minutes, freeze-drying them, and then grinding them again for 2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 25° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0078] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 15 minutes, allow to stand for 25 minutes, and then seal and reflux for 4 hours. After cooling to room temperature, a suspension is obtained. The volume ratio of ethanol in the ethanol-water solution is 15%. The mass ratio of patchouli powder to ethanol-water solution is 1:12. The ultrasonic temperature is 8°C, the ultrasonic frequency is 90kHz, and the standing temperature is 38°C. The sealed reflux temperature is 85°C, and steam is introduced to the bottom of the solution after reflux.

[0079] Step 4, distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 104° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 85° C. and the temperature of the second gradient condensation is 12° C.;

[0080] Step 5: adding a surfactant and a co-surfactant to a patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, adding water to the oil phase under high-speed stirring to prepare colostrum; injecting the prepared colostrum into a high-pressure microjet homogenizer; after homogenization, discharging and standing to obtain water-soluble patchouli essential oil; the premixing process adopts mechanical stirring, and the stirring speed is 400 r / min, and the temperature is 45° C.; the homogenization pressure of the high-pressure homogenizer is 50 MPa, the colostrum temperature is 38° C., and the number of homogenization times is 7 times; the mass fraction of the water-soluble patchouli essential oil is: 7% patchouli essential oil, 10% surfactant, 12% co-surfactant, and the balance water.

[0081] Among them, the surfactant is a compound of PPG-PPG-6-decyltetradecyl alcohol polyether-30 and polyoxyethylene 40 hydrogenated castor oil in a mass ratio of 1:2, and the co-surfactant is 1,3-propylene glycol.

[0082] Comparative Example 1

[0083] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0084] Step 1, placing patchouli in water and steaming for 12 hours, and then cooling to room temperature to obtain a suspension, wherein the mass ratio of patchouli to water is 1:12, and the steaming temperature is 100°C;

[0085] Step 4: distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through condensation. The distillation temperature is 104° C., and the condensation temperature is 12° C.

[0086] Comparative Example 2

[0087] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0088] Step 1: patchouli and ethanol are blended and wet-grinded for 3 hours, followed by vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 6:1, the wet-grinding temperature is 12° C., the grinding pressure is 0.6 MPa, and the vacuum distillation temperature is 35° C., and the pressure is 90% of atmospheric pressure;

[0089] Step 2: allowing the patchouli granules to stand for 8 minutes, freeze-drying them, and then grinding them again for 2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 25° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0090] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 15 minutes, allow to stand for 25 minutes, and then seal and reflux for 4 hours. After cooling to room temperature, a suspension is obtained. The volume ratio of ethanol in the ethanol-water solution is 15%. The mass ratio of patchouli powder to ethanol-water solution is 1:12. The ultrasonic temperature is 8°C, the ultrasonic frequency is 90kHz, and the standing temperature is 38°C. The sealed reflux temperature is 85°C, and steam is introduced to the bottom of the solution after reflux.

[0091] Step 4, distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 104° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 85° C. and the temperature of the second gradient condensation is 12° C.;

[0092] Step 5: Add a surfactant and a co-surfactant to the patchouli essential oil for premixing to prepare an oil phase. After the oil phase is completely dissolved, water is added to the oil phase under high-speed stirring to prepare colostrum. The prepared colostrum is injected into a high-pressure microfluidizer. After homogenization, the material is allowed to stand to obtain water-soluble patchouli essential oil. The premixing process uses mechanical stirring at a stirring speed of 400 r / min and a temperature of 45°C. The homogenization pressure of the high-pressure homogenizer is 50 MPa, the colostrum temperature is 38°C, and the homogenization number is 7 times. The mass fraction of the water-soluble patchouli essential oil is: 5% patchouli essential oil, 9% surfactant, 10% co-surfactant, and the balance is water. The surfactant is PPG-6-decyltetradecyl alcohol polyether-30 and the co-surfactant is 1,3-propylene glycol.

[0093] Comparative Example 3

[0094] A method for preparing water-soluble patchouli essential oil comprises the following steps:

[0095] Step 1: patchouli and ethanol are blended and wet-grinded for 3 hours, followed by vacuum distillation to obtain fine patchouli particles; the mass ratio of patchouli to ethanol is 6:1, the wet-grinding temperature is 12° C., the grinding pressure is 0.6 MPa, and the vacuum distillation temperature is 35° C., and the pressure is 90% of atmospheric pressure;

[0096] Step 2: allowing the patchouli granules to stand for 8 minutes, freeze-drying them, and then grinding them again for 2 hours to obtain patchouli fine powder, wherein the standing atmosphere is humid air at a temperature of 25° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa;

[0097] Step 3: Ultrasonicate patchouli powder in an ethanol-water solution for 15 minutes, allow to stand for 25 minutes, and then seal and reflux for 4 hours. After cooling to room temperature, a suspension is obtained. The volume ratio of ethanol in the ethanol-water solution is 15%. The mass ratio of patchouli powder to ethanol-water solution is 1:12. The ultrasonic temperature is 8°C, the ultrasonic frequency is 90kHz, and the standing temperature is 38°C. The sealed reflux temperature is 85°C, and steam is introduced to the bottom of the solution after reflux.

[0098] Step 4, distilling the suspension for 4 hours to obtain patchouli essential oil and ethanol through gradient condensation, wherein the distillation temperature is 104° C., and the gradient condensation includes a first gradient condensation and a second gradient condensation, wherein the temperature of the first gradient condensation is 85° C. and the temperature of the second gradient condensation is 12° C.;

[0099] Step 5: Add a surfactant and a co-surfactant to the patchouli essential oil for premixing to prepare an oil phase. After the oil phase is completely dissolved, water is added to the oil phase under high-speed stirring to produce colostrum. The prepared colostrum is injected into a high-pressure microfluidizer. After homogenization, the material is allowed to stand to obtain water-soluble patchouli essential oil. The premixing process uses mechanical stirring at a stirring speed of 400 r / min and a temperature of 45°C. The homogenization pressure of the high-pressure homogenizer is 50 MPa, the colostrum temperature is 38°C, and the homogenization number is 7 times. The mass fraction of the water-soluble patchouli essential oil is: 5% patchouli essential oil, 9% surfactant, 10% co-surfactant, and the balance is water. The surfactant is polyoxyethylene 40 hydrogenated castor oil, and the co-surfactant is 1,3-propylene glycol.

[0100] Comparative Example 4

[0101] Comparative Example 4 uses commercially available patchouli essential oil, which is purchased from Zhanjiang XX Spice Co., Ltd.

[0102] Examples 1-5 and Comparative Examples 1-4 were used as samples for testing, and the results were as follows:

[0103] Among them, the quantitative analysis results of the chemical components of patchouli essential oil extracted from the samples during the extraction process are as follows:

[0104] Example Patchouli alcohol content (%) Patchouli ketone content (%) Example 1 35.22 1.27 Example 2 38.05 1.61 Example 3 43.14 1.83 Example 4 43.21 1.84 Example 5 43.18 1.83 Comparative Example 1 27.88 1.89 Comparative Example 2 42.74 1.81 Comparative Example 3 42.78 1.82 Comparative Example 4 26.53 1.66

[0105] Comparison of the above data indicates that the present technical solution, in which patchouli is blended with ethanol and then wet-grinded, followed by freeze-drying and secondary grinding, yields a fine powder. The patchouli essential oil extracted from this fine powder by steam distillation exhibits excellent dissolution efficiency, with the patchouli alcohol content in the extracted patchouli essential oil ranging from 35.22% to 43.11%, and the patchouli ketone content ranging from 1.27% to 1.83%. Comparative Example 1 varied the extraction solvent and extraction ratio, while Comparative Example 4 evaluated commercially available patchouli oil and found that, compared with the extraction methods described in the examples, the method provided by the present technical solution was able to extract more active extracts, effectively improving extraction efficiency.

[0106] The results of the stability test analysis are as follows:

[0107] Example Room temperature stability Low temperature stability Centrifugal stability Example 1 Stablize Stablize Unstratified Example 2 Stablize Stablize Unstratified Example 3 Stablize Stablize Unstratified Example 4 Stablize Stablize Unstratified Example 5 Stablize Stablize Unstratified Comparative Example 1 Stablize Stablize Unstratified Comparative Example 2 Let it stand for a while and stratify / / Comparative Example 3 Let it stand for a while and stratify / / Comparative Example 4 Stablize Stablize Unstratified

[0108] The above results indicate that Examples 1-5, Comparative Examples 1, and Comparative Example 4 all produced patchouli essential oil microemulsions with good room temperature, low temperature, and centrifugation stability. Comparative Examples 2-3, in which only the composite surfactant was replaced with a single surfactant, also formed microemulsions, but all exhibited stratification after standing for a period of time, indicating that the combined surfactant is more conducive to microemulsion stability.

[0109] Example 6 Safety Evaluation of Water-Soluble Patchouli Essential Oil

[0110] Experimental Method: This study used the endpoint scoring method. Nine-day-old chicken embryos were candled and the air cell position was marked on the eggshell surface. The marked eggshell part was peeled off with dental serrated curved forceps to expose the white egg membrane. An appropriate amount of mass was taken with a pipette.

[0111] After moistening the egg membrane with a 0.9% NaCl solution, pour off the solution. Carefully remove the intima with forceps, ensuring that the vascular membrane is not damaged. Pipette 0.3 mL of the test agent (a 10% aqueous solution of the sample from Example 3) and drip it onto the chorioallantoic membrane. Observe the extent of each toxic effect over a 5-minute period.

[0112] as a result Figure 1 As shown, the test result of the aqueous solution of the water-soluble patchouli essential oil of Example 3 with a concentration of 10% was non-irritating.

[0113] Example 7 Preparation Method and Efficacy Test Results of 3% CB2 Soothing and Redness-Reducing Cream

[0114] (1) Material preparation: C14-22 alcohol / C12-20 alkyl glucoside, glyceryl stearate / PEG-100 stearate, caprylic / capric triglyceride, shea butter (BUTYROSPERMUM PARKII), squalane, dimethicone; Phase B: acrylates / C10-30 alkyl acrylate crosspolymer, acrylates / C10-30 alkyl acrylate crosspolymer, hydrogenated lecithin, acetyl glucosamine, butylene glycol, glycerin, preservative; Phase C: arginine, Phase D: water-soluble patchouli essential oil; Phase E: carnosine; purified water: balance.

[0115] (2) Preparation of 3% CB2 soothing and redness-reducing cream: weigh phase A in a beaker, heat to 85°C and dissolve evenly without particles; dissolve phase C and phase E in water separately for later use; then weigh phase B in another beaker, heat to 85°C and dissolve, keep warm for 30 minutes, start homogenization, and homogenize for 3-5 minutes until it is even and without particles; pour phase A into phase B, homogenize for 3 minutes until it is even and without particles; add dissolved phase C, stir and cool; stir and cool to below 45°C, add phase D and phase E in turn and stir evenly to complete the preparation.

[0116] Effective effect verification:

[0117] (I) In vitro soothing efficacy test of 3% CB2 soothing redness-reducing cream

[0118] This test uses CAP to stimulate the 3D epidermal skin model The soothing effect of the test sample was evaluated by detecting the changes in the content of capsaicin receptor (TRPV1) and cannabinoid receptor 2 (CB2).

[0119] Table 1 Test Group

[0120]

[0121] Test steps: First, according to the test group, the model was transferred to a 6-well plate with 0.9 mL of EpiGrowth culture medium added in advance, and the test group number was marked. Among them, the BC group did not receive any treatment, the NC group, PC group and sample group added EpiGrowth culture medium containing 15 μM CAP submerged, the PC group added the corresponding concentration of positive control working solution, and the sample group evenly distributed the sample working solution on the model surface. The 6-well plate was placed in a CO2 incubator (37°C, 5% CO2) and incubated for 24 hours; after the incubation, the residual test substance was washed with sterile PBS solution, and the residual liquid was wiped off with a sterile cotton swab; then, the model for testing was taken and fixed with 4% paraformaldehyde. After fixation for 24 hours, immunofluorescence detection was performed, and photos were taken under a microscope for observation, collection and analysis.

[0122]

[0123] 1. Summary table of TRPV1 immunofluorescence analysis results

[0124] Group Relative IOD average SD P-value Inhibition rate (vs NC) BC 1.00 0.17 / / NC 1.67 0.15 0.006## / PC 1.03 0.19 0.009** 38.32％ 3% CB2 Soothing Redness-Reducing Cream 0.80 0.08 0.001** 52.10％

[0125] Note: The integrated optical density (IOD) reflects the content of TRPV1. When statistically analyzed using the t-test method, significance is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##. When compared with the BC group, significance is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0126] Analysis of TRPV1 immunofluorescence results:

[0127] Compared with the BC group, the TRPV1 content of the NC group increased significantly, indicating that the stimulation conditions of this test were effective; compared with the NC group, the TRPV1 content of the PC group decreased significantly, indicating that the positive control of this test was effective; compared with the NC group, the TRPV1 content of the sample 3% CB2 soothing and redness-reducing cream decreased significantly, with an inhibition rate of 52.10%.

[0128]

[0129] 2. CB2 Immunofluorescence Analysis Results Summary Table

[0130] Group Relative IOD average SD P-value Inhibition rate (vs NC) BC 1.00 0.20 / / NC 0.33 0.05 0.005## / PC 1.02 0.12 0.001** 209.09％ 3% CB2 Soothing Redness-Reducing Cream 1.28 0.10 0.000** 287.88％

[0131] Note: The integrated optical density (IOD) reflects the content of TRPV1. When statistically analyzed using the t-test method, significance is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##. When compared with the BC group, significance is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0132] Compared with the BC group, the CB2 content of the NC group decreased significantly, indicating that the stimulation conditions of this test were effective; compared with the NC group, the CB2 content of the PC group increased significantly, indicating that the positive control of this test was effective; compared with the NC group, the CB2 content of the sample 3% CB2 soothing and redness-reducing cream increased significantly, with an increase rate of 287.88%.

[0133] In vitro soothing efficacy test conclusion:

[0134] Based on 3D epidermal skin model Compared with the control group, the capsaicin receptor (TRPV1) content of the sample 3% CB2 soothing and redness-reducing cream was significantly decreased, with an inhibition rate of 52.10%, and the cannabinoid receptor 2 content was significantly increased, with an increase rate of 287.88%, indicating that the sample can reduce the capsaicin receptor (TRPV1) content and increase the cannabinoid receptor 2 content, and has a soothing effect.

[0135] (II) Human Efficacy Test of 3% CB2 Soothing and Redness-Reducing Cream

[0136] Test subjects: 33 healthy Chinese women aged 18-50, with self-identified sensitive skin, a lactic acid sting index ≥ 3 points, poor facial barrier function, prone to redness, and normal to oily skin.

[0137] Testing indicators: transepidermal water loss value, skin redness a* value, skin red area ratio and subject self-assessment.

[0138] Testing method: Using the before-after comparison method, measure the transepidermal water loss value of the subject's skin, conduct subjective evaluation and take photos of the subject's full face, such as Figure 2 As shown,

[0139] (1) Summary table of transepidermal water loss, skin redness a* value, and skin red area ratio

[0140]

[0141] Significance marking method: "ns" indicates no statistical difference, p≥0.05; p<0.05 indicates a significant difference.

[0142] Result analysis:

[0143] After using 3% CB2 soothing and anti-erythema cream for 14 consecutive days, the transepidermal water loss value of the skin was significantly reduced by 8.48%; after using 3% CB2 soothing and anti-erythema cream for 28 consecutive days, the transepidermal water loss value of the skin was significantly reduced by 15.55%.

[0144] After using 3% CB2 soothing and anti-erythema cream for 14 consecutive days, the VISIA skin redness a* value significantly decreased by 3.10%; after using 3% CB2 soothing and anti-erythema cream for 28 consecutive days, the VISIA skin redness a* value significantly decreased by 6.92%.

[0145] After using 3% CB2 Soothing Redness Relief Cream for 14 consecutive days, VISIA's skin redness area decreased significantly by 7.13%.

[0146] After using 3% CB2 soothing and anti-redness cream for 28 consecutive days, the area of ​​redness on VISIA's skin decreased significantly by 9.50%.

[0147] (2) Analysis of subjects’ self-assessment results

[0148] After continuous use of the test product 3% CB2 Soothing Redness-Reducing Cream for 28 days, 100.00% of the subjects felt that their skin was more hydrated; 100.00% of the subjects felt that their skin dryness was improved; 96.97% of the subjects felt that their skin redness was improved; 100.00% of the subjects felt that their skin condition was more stable; 96.97% of the subjects felt that the product could soothe skin discomfort; 100.00% of the subjects felt that the product had a soothing effect; 100.00% of the subjects felt that the product could repair the skin barrier; 100.00% of the subjects felt that the product had a repairing effect; 100.00% of the subjects felt that the product was gentle and non-irritating; 100.00% of the subjects felt that the product was suitable for sensitive skin; and 100.00% of the subjects were generally satisfied with the product.

[0149] Based on the soothing and restorative efficacy data from human subjects, the test product, 3% CB2 Soothing Redness-Reducing Cream, was shown to have soothing and repairing properties after continuous use for 14 and 28 days. 100.00% of subjects felt the product was mild and non-irritating, and 100.00% of subjects felt it was suitable for sensitive skin, indicating that the test product is mild and non-irritating, making it suitable for sensitive skin.

[0150] Example 8: Preparation Method and Efficacy Test Results of 0.5% CB2 Soothing and Redness-Reducing Facial Mask

[0151] (1) Material preparation: Phase A: 0.03% hydroxyethyl cellulose, 0.18% carbomer, 0.05% disodium EDTA, 0.05% dipotassium glycyrrhizate, 1% trehalose, 0.5% acetyl glucosamine, 0.2% allantoin, 7% glycerol, 1% butylene glycol, 0.8% preservative; Phase B: 0.21% arginine; Phase C: 0.3% water-soluble patchouli essential oil; Purified water: balance.

[0152] (2) Preparation of 0.5% CB2 soothing and redness-reducing facial mask: Weigh phase A, heat to 85°C to dissolve completely, and keep warm for 30 minutes; dissolve phase B separately in water and set aside; start homogenizing phase A, homogenize for 3-5 minutes until the material is uniform and free of particles, add phase B, stir and cool to 45°C, weigh phase C, stir evenly; and discharge.

[0153] Effective effect verification:

[0154] Human Efficacy Testing of a 0.5% CB2 Soothing and Redness-Reducing Mask

[0155] Test subjects: 33 healthy Chinese women aged 18-50, with self-identified sensitive skin, a lactic acid sting index ≥ 3 points, poor facial barrier function, prone to redness, and normal to oily skin.

[0156] Detection indicators: transepidermal water loss value, skin redness a* value, and skin red area ratio.

[0157] Test method: Using the self-before and after control and blank control method, measure the transepidermal water loss value of the subject's skin, make a subjective evaluation and take a full-face photo of the subject, such as Figure 3 shown.

[0158]

[0159] Test results:

[0160] 20 minutes after the subjects used the test product once, the changes in the transepidermal water loss value of the skin in the test product area, the changes in the VISIA skin redness a* value, and the changes in the VISIA skin red zone area ratio were lower than those in the blank control area.

[0161] In summary, water-soluble patchouli essential oil has soothing and repairing effects. The product is mild and non-irritating, suitable for sensitive skin, and can effectively improve the redness of the skin.

[0162] It is understood that the above specific description of the present invention is only used to illustrate the present invention and is not limited to the technical solutions described in the embodiments of the present invention. Those skilled in the art should understand that the present invention can still be modified or replaced with equivalents to achieve the same technical effects; as long as the use requirements are met, they are all within the scope of protection of the present invention.

Claims

1. A method for preparing a water-soluble patchouli essential oil, characterized in that: The steps include: Step 1: patchouli is mixed with ethanol and then wet-grinded for 1-4 hours, followed by vacuum distillation to obtain fine patchouli particles; Step 2, allowing the patchouli granules to stand for 5-10 minutes, freeze-drying them, and then grinding them again for 1-2 hours to obtain patchouli fine powder; Step 3, placing the patchouli powder in an ethanol aqueous solution and ultrasonicating for 10-20 minutes, allowing to stand for 20-30 minutes, then sealing and refluxing for 3-5 hours, and cooling to room temperature to obtain a suspension; Step 4, distilling the suspension for 3-4 hours, and obtaining patchouli essential oil and ethanol through gradient condensation; Step 5: Add a surfactant and a co-surfactant to the patchouli essential oil liquid for premixing to prepare an oil phase; after the oil phase is completely dissolved, add water to the oil phase under high-speed stirring to prepare colostrum; inject the prepared colostrum into a high-pressure microfluidizer; after homogenization, discharge the material and let it stand to obtain water-soluble patchouli essential oil.

2. The method for preparing water-soluble patchouli essential oil according to claim 1, wherein: The mass ratio of patchouli to ethanol in step 1 is 5-8:1, the temperature of the wet grinding treatment is 10-15° C., the grinding pressure is 0.5-0.7 MPa, the temperature of the reduced pressure distillation is 30-40° C., and the pressure is 90% of atmospheric pressure.

3. The method for preparing water-soluble patchouli essential oil according to claim 1, wherein: In the step 2, the static atmosphere is humid air at a temperature of 20-30° C.; the freeze-drying temperature is −20° C., and the grinding pressure is 0.6 MPa.

4. The method for preparing water-soluble patchouli essential oil according to claim 1, wherein: The volume ratio of ethanol in the ethanol-water solution in step 3 is 10-20%. The mass ratio of patchouli powder to ethanol-water solution is 1:10-15. The ultrasonic temperature is 5-10°C, the ultrasonic frequency is 80-100 kHz, and the static temperature is 35-40°C. The sealed reflux temperature is 80-90°C, and steam is introduced to the bottom of the solution after reflux.

5. The method for preparing water-soluble patchouli essential oil according to claim 1, wherein: The temperature of the distillation treatment in step 4 is 103-105°C, and the gradient condensation includes a first gradient condensation and a second gradient condensation. The temperature of the first gradient condensation is 80-90°C, and the temperature of the second gradient condensation is 10-15°C.

6. The method for preparing the water-soluble patchouli essential oil according to claim 1, wherein: The premixing treatment in step 5 adopts mechanical stirring with a stirring speed of 300-500 r / min and a temperature of 40-50° C.; the homogenization pressure of the high-pressure homogenizer is 40-60 MPa, the colostrum temperature is 35-40° C., and the homogenization times are 6-8 times; the mass fraction of the water-soluble patchouli essential oil is: 2-10% patchouli essential oil, 3-15% surfactant, 2-15% cosurfactant, and the balance water.

7. The method for preparing the water-soluble patchouli essential oil according to claim 6, wherein: The surfactant is a composition of polyoxyethylene 40 hydrogenated castor oil and PPG-6-decyltetradecyl alcohol polyether-30.

8. The method for preparing the water-soluble patchouli essential oil according to claim 6, wherein: The co-surfactant is one or more of n-propanol, 1,2-propylene glycol and 1,3-propylene glycol.

9. The method for preparing the water-soluble patchouli essential oil according to claim 1, wherein: The water-soluble patchouli essential oil can be used to prepare a soothing and redness-reducing cream.

10. The method for preparing the water-soluble patchouli essential oil according to claim 1, wherein: The water-soluble patchouli essential oil can be used to prepare a soothing and redness-reducing facial mask liquid.

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