Novel split storage type oligopeptide freeze-dried powder and essence combined skin-brightening skin care product

Abstract

The invention relates to the technical field of skin care products. The invention aims to provide a split storage type novel skin-brightening skin-care product combined by oligopeptide freeze-dried powder and essence. The split storage type novel skin-brightening skin-care product comprises multiple oligopeptide freeze-dried powder and solvent essence, the multi-oligopeptide freeze-dried powder is prepared from the following raw materials in parts by weight: 90 to 95 parts of water, 1 to 2 parts of glutathione, 1 to 2 parts of mannitol, 1 to 2 parts of carnosine, 0.5 to 1 part of ergothioneine, 0.5 to 1 part of vitamin C and 0.0005 to 0.001 part of a multi-peptide-zinc chelate. The multi-peptide-zinc chelate is prepared from the following raw materials in parts by weight: 1-2 parts of hexapeptide-2, 1-2 parts of nonapeptide-1, 1-2 parts of dipeptide-2, 1-2 parts of tripeptide-10 and 2-3 parts of zinc oxide. According to the invention, various peptides with small molecular weights are chelated with zinc, the characteristics of good permeability and strong skin affinity of the small molecular peptides are fully played, the effects of zinc and other components are effectively played, and the overall use effect is greatly improved. Through compounding of multiple components, the moisturizing and brightening mask has multiple effects of moisturizing, good permeability, whitening, brightening and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence. Background Art

[0002] As the human body ages, skin aging issues also arise. In recent years, the combined effects of high work and life pressures, severe environmental pollution, and lack of sleep in modern society have made skin aging issues more pronounced, leading to an increasing demand for skin care and repair products. Oligopeptides, a type of peptide, generally have a molecular weight below 1000 Daltons. They are also called small peptides, oligopeptides, oligosaccharides, or small molecule active peptides, and are generally composed of 2-10 amino acids. They possess excellent antioxidant properties, can scavenge free radicals, and reduce oxidative damage, and their efficacy in delaying cellular aging is gaining increasing attention. Because oligopeptides are directly absorbed by the human body without digestion, and zinc, a key component of various enzymes in the body, possesses excellent properties such as antibacterial and anti-inflammatory properties, and promotes cell growth and repair, combining the two offers the potential to fully leverage their respective strengths and synergistically enhance skin anti-aging properties. However, to date, no relevant reports have been found in this field. Summary of the Invention

[0003] The purpose of the present invention is to provide a novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence, which can reduce skin oxidative damage and enhance skin anti-aging properties.

[0004] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence, comprising multiple oligopeptide freeze-dried powders and a solvent essence;

[0005] The multiple oligopeptide freeze-dried powder is prepared from the following raw materials in parts by weight: 90-95 parts of water, 1-2 parts of glutathione, 1-2 parts of mannitol, 1-2 parts of carnosine, 0.5-1 parts of ergothioneine, 0.5-1 parts of vitamin C, and 0.0005-0.001 parts of a multiple peptide-zinc chelate; the multiple peptide-zinc chelate is prepared from the following raw materials in parts by weight: 1-2 parts of hexapeptide-2, 1-2 parts of nonapeptide-1, 1-2 parts of dipeptide-2, 1-2 parts of tripeptide-10, and 2-3 parts of zinc oxide.

[0006] Preferably, the solvent essence contains the following raw materials in parts by weight: 90-95 parts of water, 0.5-1 part of 1,2-pentanediol, 1-2 parts of purslane extract, 1-2 parts of Centella asiatica extract, 0.5-1 part of green tea extract, 0.5-1 part of p-hydroxyacetophenone, 0.1-0.3 parts of glycerol, 0.1-0.3 parts of glyceryl glucoside, 0.2-0.5 parts of betaine, and 0.1-0.3 parts of butylene glycol.

[0007] Preferably, the solvent essence further comprises the following raw materials in parts by weight: 0.005-0.01 parts of acetylated sodium hyaluronate and 0.01-0.03 parts of Stephania tetrandra extract.

[0008] Preferably, the multiple oligopeptide lyophilized powder is prepared by the following method: weighing the raw materials in parts by weight according to the formula, mixing 1 / 5 of the weighed water with the multiple peptide-zinc chelate by weight, heating to 40-60°C within 2 minutes, and stirring continuously for 30 minutes; after stirring, rapidly cooling to room temperature within 2 minutes; repeating heating-stirring-cooling 3 times to obtain a mixed solution A; adding other raw materials to the remaining water, mixing and stirring uniformly to obtain a mixed solution B; combining the mixed solution A and the mixed solution B, and stirring continuously for 2 hours; then lyophilizing to a water content of ≤5% in a vacuum freezer, and packaging into vials at 350 mg / bottle to obtain the multiple oligopeptide lyophilized powder.

[0009] Preferably, the multi-peptide-zinc chelate is prepared by the following method: zinc oxide powder is weighed in parts by weight and added to distilled water, the pH value is adjusted to 6 with acetic acid to prepare a 0.5 mol / L zinc oxide solution; hexapeptide-2, nonapeptide-1, dipeptide-2, and tripeptide-10 are weighed in parts by weight and added to the zinc oxide solution; the mixture is stirred and reacted at 10-15° C. for 36-48 hours to obtain a zinc-peptide mixture, and the zinc-peptide mixture is placed in a vacuum freezer and freeze-dried to a freeze-dried powder with a water content of ≤3%, thereby obtaining a multi-peptide-zinc chelate.

[0010] Preferably, the solvent essence is prepared by the following method: weighing raw materials in parts by weight according to the formula, mixing and stirring evenly; and filling into vials at a rate of 7 ml / bottle to obtain the solvent essence.

[0011] Preferably, after opening the package, pour the solvent essence into the multiple oligopeptide freeze-dried powder, shake to mix evenly, and take 6-10 drops each time and apply evenly on the facial skin.

[0012] The beneficial effects of the present invention are concentrated in:

[0013] 1. Through the introduction of multiple peptide-zinc chelates, it has good antioxidant properties, can effectively remove free radicals and delay aging.

[0014] 2. Through the addition of trace element zinc, zinc, as an important component of various enzymes in the human body, can promote the growth and repair of skin cells, increase skin elasticity, delay aging; prevent pigment deposition, and make the skin firmer and brighter.

[0015] 3. Chelate a variety of small molecular weight peptides with zinc to give full play to the good permeability and strong skin affinity of small molecule peptides, effectively exert the effects of zinc and other components, and greatly improve the overall use effect.

[0016] 4. Through the compounding of multiple components, the present invention has multiple functions such as moisturizing, good permeability, whitening and brightening. DETAILED DESCRIPTION

[0017] The present invention is a combination product, which contains both a multiple oligopeptide freeze-dried powder and a solvent essence. The two products are stored separately in a vial. When in use, after opening the package, pour the solvent essence into the multiple oligopeptide freeze-dried powder, shake to mix evenly, and take 6-10 drops each time and apply evenly to the facial skin. It can also be used with a corresponding essence lotion, mixed evenly with it and applied to the facial skin. It should be noted that the multiple oligopeptide freeze-dried powder of the present invention should be used promptly after opening and mixing with the solvent essence, generally used up within 5-10 days, and stored in a cool and shady place at low temperature.

[0018] Multiple oligopeptide lyophilized powder:

[0019] The multiple oligopeptide freeze-dried powder of the present invention is prepared from the following raw materials in parts by weight: 90-95 parts of water, 1-2 parts of glutathione, 1-2 parts of mannitol, 1-2 parts of carnosine, 0.5-1 part of ergothioneine, 0.5-1 part of vitamin C, and 0.0005-0.001 part of multiple peptide-zinc chelate.

[0020] The multi-peptide-zinc chelate is an extremely important core trace additive of the present invention. It is made of the following raw materials in parts by weight: 1-2 parts of hexapeptide-2, 1-2 parts of nonapeptide-1, 1-2 parts of dipeptide-2, 1-2 parts of tripeptide-10, and 2-3 parts of zinc oxide. During preparation: zinc oxide powder is weighed by weight and added to distilled water, and the pH value is adjusted to 6 with acetic acid to prepare a 0.5 mol / L zinc oxide solution. Hexapeptide-2, nonapeptide-1, dipeptide-2, and tripeptide-10 are weighed by weight and added to the zinc oxide solution; the reaction is stirred at low temperature at 10-15°C for 36-48 hours to obtain a zinc-peptide mixture. This low-temperature stirring can effectively prevent the decomposition and denaturation of the oligopeptides, avoid affecting their structure and function, ensure that they maintain good absorption rate and bioavailability, and ensure the stability of the chelate. The zinc-peptide mixture is placed in a vacuum freezer and freeze-dried to a freeze-dried powder with a water content of ≤3%, thereby obtaining a multiple peptide-zinc chelate (freeze-dried powder dosage form).

[0021] After the preparation of the multi-peptide-zinc chelate is completed, the multi-oligopeptide lyophilized powder of the present invention is prepared as follows: weigh the raw materials in parts by weight according to the formula, take 1 / 5 of the total weight of water, and mix it with the multi-peptide-zinc chelate. Use an intermittent rapid heating and cooling dissolution method, heat to 40-60°C within 2 minutes, and continue stirring for 30 minutes; after stirring, quickly cool to room temperature within 2 minutes; repeat heating-stirring-cooling 3 times to obtain a mixed solution A; this method can improve the stirring and mixing efficiency while ensuring the invariance of the components. Add other raw materials to the remaining water, mix and stir evenly to obtain a mixed solution B; combine the mixed solution A and the mixed solution B, and continue stirring for 2 hours; then freeze-dry to a water content of ≤5% in a vacuum freezer, and dispense into syringe bottles at 350 mg / bottle to obtain a multi-oligopeptide lyophilized powder.

[0022] Solvent Essence

[0023] The solvent essence of the present invention is used to dissolve freeze-dried powder of multiple oligopeptides during use, improving ease of use and bioavailability. It comprises: 90-95 parts water, 0.5-1 part 1,2-pentanediol, 1-2 parts purslane extract, 1-2 parts Centella asiatica extract, 0.5-1 part green tea extract, 0.5-1 part p-hydroxyacetophenone, 0.1-0.3 parts glycerol, 0.1-0.3 parts glyceryl glucoside, 0.2-0.5 parts betaine, and 0.1-0.3 parts butylene glycol. The solvent essence is prepared by weighing the raw materials in parts by weight according to the formula, mixing and stirring uniformly, and then filling into vials at a rate of 7 ml per bottle to obtain the solvent essence. In order to better improve the moisturizing and antibacterial and anti-inflammatory properties, acetylated sodium hyaluronate and Stephania tetrandra can also be added to the solvent evolution liquid, with 0.005-0.01 parts of acetylated sodium hyaluronate and 0.01-0.03 parts of Stephania tetrandra extract.

[0024] The technical solution of the present invention is further described below by way of specific embodiments. It should be understood by those skilled in the art that the embodiments are merely to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0025] In Examples 1-3, multiple peptide-zinc chelates were prepared according to the method of the present invention and the ratios shown in Table 1.

[0026] Table 1

[0027] Hexapeptide-2 Nonapeptide-1 Dipeptide-2 Tripeptide-10 zinc oxide Example 1 1 2 1 2 2 Example 2 2 1 2 1 3 Example 3 1 1 2 2 3

[0028] In Examples 4-7, multiple oligopeptide freeze-dried powders and solvent essences were prepared according to the method of the present invention and the ratios in Table 2.

[0029] Table 2

[0030]

[0031]

[0032] The only difference between Control Example 1 and Example 7 is that the multi-peptide-zinc chelate prepared in Example 1 was not added.

[0033] The only difference between Control Example 2 and Example 7 is that the same components as in Example 1, namely, 1 part hexapeptide-2, 2 parts nonapeptide-1, 1 part dipeptide-2, 2 parts tripeptide-10, and 2 parts zinc oxide, are used to prepare an unchelated multi-peptide and zinc oxide mixture without performing separate peptide-metal liquid chelation; and the mixture is then directly mixed with the other raw materials in proportion.

[0034] The only difference between Comparative Example 3 and Comparative Example 2 is that zinc oxide is not added.

[0035] The only difference between Control Example 4 and Control Example 2 is that hexapeptide-2, nonapeptide-1, dipeptide-2, and tripeptide-10 are not added.

[0036] Test Example 1

[0037] Using an in vitro antioxidant test, a free radical scavenging test was performed on the products prepared in Examples 4-7 and Control Examples 1-4.

[0038] Test sample:

[0039] 50 mg of the lyophilized powder of the multiple oligopeptides in Examples 4-7 were respectively dissolved in 1 ml of the solvent essence in Examples 4-7 to prepare samples 1-4;

[0040] In the same manner, 50 mg of the lyophilized powder in Control Examples 1-4 was dissolved in 1 ml of the corresponding solvent to prepare Samples 5-8.

[0041] Test Method: Add 5 mL of 0.2 mol / L DPPH solution to each of Samples 1-8; shake well and incubate in the dark for 30 minutes. Measure the absorbance (A1) at 515 nm. Simultaneously, measure the absorbance (A0) at 515 nm after mixing 5 mL of the same DPPH solution with 1 mL of pure water. Free radical scavenging ability: DPPH scavenging percentage = (A0 - A1) / A0 × 100%.

[0042] Test results: as shown in Table 3.

[0043] Table 3 DPPH solution free radical scavenging rate

[0044] Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Clearance 62.54％ 67.84％ 72.63％ 78.65％ 39.32％ 58.64％ 54.65％ 62.69％

[0045] As can be seen from Table 3, the present invention has a good free radical scavenging ability as a whole through its unique compounding. The introduction of multiple oligopeptides and zinc further enhances the free radical scavenging ability of the present invention, has an excellent effect on improving the skin's antioxidant capacity, and has a unique anti-aging effect.

[0046] Test Example 2

[0047] Human skin anti-aging experiment

[0048] Test method:

[0049] 160 female volunteers aged 30-35 with healthy skin were randomly divided into 8 groups. Each group was assigned to use samples 1-8, respectively. After cleansing their faces morning and evening, the volunteers applied 5 drops of each sample using a dropper of the same size to the skin around their eyes. Massage for 5-10 minutes twice daily for 10 consecutive days. Following follow-up, each group was evaluated based on satisfaction with improved skin elasticity (weighted 30%), whitening (weighted 30%), and fine line improvement (weighted 40%). The highest and lowest scores were removed from each group to calculate the average of each data point. The results are shown in Table 4.

[0050] Table 4

[0051] Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group 8 Skin elasticity 83 82 90 92 26 68 63 38 Whitening improvement 79 78 69 71 54 69 62 48 Fine lines improvement 79 86 82 87 37 64 59 44 Overall score 80.2 82.4 80.5 83.7 38.8 66.7 61.1 43.4

[0052] According to Table 4, it can be seen that the comprehensive evaluation of the products obtained in groups 1-4 (that is, Examples 4-7) in application is relatively high, all reaching more than 80 points, which is much higher than Group 5. It is particularly noteworthy that, in combination with Table 3, it can be seen that in the in vitro free radical scavenging experiment, Examples 4-7 are improved compared with Control Example 4, but the maximum improvement rate is about 25% (Example 7 and Control Example 4); However, in actual use, the satisfaction scores of Groups 1-4 are significantly increased compared with Group 8, and the maximum improvement rate even reaches about 93%. This shows that when applied to the human body, small molecule oligopeptides, as high-quality carriers of zinc, can greatly improve the permeability and absorption rate of zinc, give full play to the enzymatic effect of zinc in the body, fully activate the cell's own activity and repair ability, and greatly improve the overall use effect of the product.

Claims

1. A novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence, characterized by: Including multiple oligopeptide freeze-dried powder and solvent essence; The multiple oligopeptide freeze-dried powder is prepared from the following raw materials in parts by weight: 90-95 parts of water, 1-2 parts of glutathione, 1-2 parts of mannitol, 1-2 parts of carnosine, 0.5-1 parts of ergothioneine, 0.5-1 parts of vitamin C, and 0.0005-0.001 parts of a multiple peptide-zinc chelate; the multiple peptide-zinc chelate is prepared from the following raw materials in parts by weight: 1-2 parts of hexapeptide-2, 1-2 parts of nonapeptide-1, 1-2 parts of dipeptide-2, 1-2 parts of tripeptide-10, and 2-3 parts of zinc oxide.

2. The novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence according to claim 1, characterized in that: The solvent essence contains the following raw materials in parts by weight: 90-95 parts of water, 0.5-1 part of 1,2-pentanediol, 1-2 parts of purslane extract, 1-2 parts of Centella asiatica extract, 0.5-1 part of green tea extract, 0.5-1 part of p-hydroxyacetophenone, 0.1-0.3 parts of glycerol, 0.1-0.3 parts of glyceryl glucoside, 0.2-0.5 parts of betaine, and 0.1-0.3 parts of butylene glycol.

3. The novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence according to claim 2, characterized in that: The solvent essence further comprises the following raw materials in parts by weight: 0.005-0.01 parts of acetylated sodium hyaluronate and 0.01-0.03 parts of Stephania tetrandra extract.

4. The novel skin-lightening skin care product comprising a split storage type freeze-dried oligopeptide powder and an essence according to any one of claims 1 to 3, characterized in that: The multi-oligopeptide lyophilized powder is prepared by the following method: weighing raw materials in parts by weight according to the formula, mixing 1 / 5 of the weighed water with the multi-peptide-zinc chelate, heating to 40-60° C. within 2 minutes, and continuously stirring for 30 minutes; and rapidly cooling to room temperature within 2 minutes after the stirring is completed; Repeat heating-stirring-cooling 3 times to obtain mixed solution A; add other raw materials to the remaining water, mix and stir evenly to obtain mixed solution B; combine mixed solution A and mixed solution B, and continue stirring for 2 hours; then lyophilize to a water content of ≤5% in a vacuum freezer, and dispense into vials at 350 mg / bottle to obtain multiple oligopeptide lyophilized powder.

5. The novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence according to claim 4, characterized in that: The multi-peptide-zinc chelate is prepared by the following method: zinc oxide powder is weighed in parts by weight and added to distilled water, and the pH value is adjusted to 6 with acetic acid to prepare a 0.5 mol / L zinc oxide solution; hexapeptide-2, nonapeptide-1, dipeptide-2, and tripeptide-10 are weighed in parts by weight and added to the zinc oxide solution; the mixture is stirred and reacted at 10-15° C. for 36-48 hours to obtain a zinc-peptide mixture, and the zinc-peptide mixture is placed in a vacuum freezer and freeze-dried to a freeze-dried powder with a water content of ≤3%, thereby obtaining the multi-peptide-zinc chelate.

6. The novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence according to claim 5, characterized in that: The solvent essence liquid is prepared by the following method: weighing raw materials in parts by weight according to the formula, mixing and stirring evenly; and filling into a penicillin bottle at a rate of 7 ml / bottle to obtain the solvent essence liquid.

7. The novel skin-lightening skin care product comprising a split storage type oligopeptide freeze-dried powder and an essence according to claim 6, characterized in that: After opening, pour the solvent essence into the multiple oligopeptide freeze-dried powder, shake to mix evenly, and apply 6-10 drops evenly on the facial skin each time.

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