Wound repair spray containing zinc hyaluronate as well as preparation method and application of wound repair spray

Abstract

The invention belongs to the technical field of chemicals, and particularly relates to a zinc hyaluronate-containing wound repair spray as well as a preparation method and application thereof. The wound repair spray containing zinc hyaluronate is prepared from the following components in percentage by mass: 0.05 to 5 percent of low molecular weight zinc hyaluronate, 0.05 to 5 percent of high molecular weight zinc hyaluronate, 0.01 to 1 percent of synergistic component, 2.0 to 8.0 percent of glycerol, 1.0 to 5.0 percent of propylene glycol, 0.05 to 0.5 percent of alpha-glucan oligosaccharide, 0.1 to 1.0 percent of serine, 0.05 to 0.3 percent of water-soluble polymer and the balance of water. The prepared wound repairing spray containing the zinc hyaluronate can be combined with a vacuum sealing drainage technology for use, and can promote rapid healing of pressure injury.

Description

Technical Field

[0001] The invention belongs to the technical field of chemical medicines, and particularly relates to a wound repair spray containing zinc hyaluronate, and a preparation method and application thereof. Background Art

[0002] Pressure injuries are surgical conditions caused by prolonged localized tissue compression, resulting in persistent ischemia, hypoxia, and malnutrition, ultimately leading to tissue ulceration and necrosis. Because pressure injuries are more common in the elderly and high-risk patients with chronic illnesses who are bedridden for extended periods, they are often accompanied by damage to skin integrity and a certain amount of normal tissue loss. This leads to long treatment times, high recurrence rates, and significant difficulty in treatment. This increases the workload for nurses and incurs expensive medical consumables. These injuries not only consume significant manpower, material, and financial resources, but can also expose patients to unexpected infections.

[0003] Hyaluronic acid is a bioactive substance widely present in the human body. It is a high-molecular-weight mucopolysaccharide composed of disaccharide units consisting of glucuronic acid and acetylglucosamine. Its primary functions in the body include maintaining the extracellular matrix environment and promoting cell repair. Recent studies have demonstrated hyaluronic acid's excellent biocompatibility and biological activity in wound healing, leading to its increasing application in wound dressings and tissue engineering, with sodium hyaluronate being the most widely used clinically. Pressure injury wounds are often complicated by infection, and anti-infection is also crucial for wound healing, but sodium hyaluronate is relatively deficient in this regard. Zinc is an essential trace element for all biological systems. Studies have shown that improving zinc status, either systemically or locally at the wound site, can promote wound repair in various types of wounds. Furthermore, zinc is a safe and effective antimicrobial metal with strong skin astringency and bactericidal properties, making it effective in treating surgical and wound infections.

[0004] Hyaluronate zinc, as an emerging biomaterial, has shown great potential in wound repair. At present, there are many reports at home and abroad that hyaluronate zinc promotes wound healing. For example, Chinese patent CN112773816B discloses a wound repair spray and its preparation method and use. The wound repair spray can greatly enhance the effect of bioactive glass in promoting wound healing and wound repair, and is easy to use, stable and low in irritation. It is particularly suitable for treating soft tissue ulcers or long-term erosions such as diabetic ulcers, diabetic foot, bedsores, sinus tracts or fat liquefaction wounds. Chinese patent CN114191597B discloses a liquid dressing for skin wound repair and its preparation method and use, which can protect skin wounds from external pollutants and bacterial invasion, while also playing a role in moisturizing and repairing wound skin.

[0005] Currently, negative pressure seal drainage (VSD) technology is a commonly used treatment method for treating this type of wound in clinical practice. It promotes blood circulation, reduces edema, accelerates granulation tissue growth, and removes wound exudate by applying negative pressure on the wound, thereby helping the wound to heal. However, although VSD technology has shown certain efficacy in treating pressure injuries, it has the problem of slow effectiveness. For some patients, especially those with chronic diseases such as diabetes or arteriosclerosis that affect blood circulation, the use of VSD technology may not quickly see significant results, mainly because these conditions will further slow down the natural repair process of the wound. At present, in order to enrich and expand the treatment strategy for pressure injury wounds, some hospitals have adopted combined therapy: in addition to VSD, other auxiliary treatment methods are introduced, such as silver ion dressings, growth factors, enzyme debridements, etc., to enhance the treatment effect.

[0006] Wnt signaling is the earliest molecular signal to appear during skin development and is the most important signaling pathway in skin damage and repair. The Wnt / β-catenin signaling pathway is the canonical Wnt signaling pathway, and its molecular mechanism is highly conserved across species. Its primary characteristic is the regulation of cytoplasmic β-catenin stability, which is involved in regulating fate, proliferation, and apoptosis. Existing research has shown that the Wnt / β-catenin signaling pathway is closely related to skin development and wound healing, and is involved in all aspects of wound healing. The inventors' previous research has shown that zinc hyaluronate can, to a certain extent, promote the repair of pressure-induced wounds by regulating the Wnt / β-catenin signaling pathway. However, its combination with VSD technology has been less effective. Building on their previous research into the molecular mechanism of zinc hyaluronate's effect on pressure wound repair, the inventors hope to further improve the clinical effectiveness of pressure wound repair by enhancing products used in combination with VSD. Summary of the Invention

[0007] The purpose of the present invention is to provide a wound repair spray containing zinc hyaluronate, and a preparation method and application thereof.

[0008] In order to achieve the above object, the present invention provides the following technical solutions:

[0009] A wound repair spray containing zinc hyaluronate comprises the following components in percentage by mass: 0.05%-5% of low molecular weight zinc hyaluronate, 0.05%-5% of high molecular weight zinc hyaluronate, 0.01%-1% of a synergistic component, 2.0%-8.0% of glycerol, 1.0%-5.0% of propylene glycol, 0.05%-0.5% of α-glucan oligosaccharide, 0.1%-1.0% of serine, 0.05%-0.3% of a water-soluble polymer, and the mixture is supplemented with water to 100%.

[0010] Preferably, the molecular weight of the low molecular weight zinc hyaluronate is ≤ 100,000 Daltons.

[0011] Preferably, the molecular weight of the low molecular weight zinc hyaluronate is 10,000-50,000 Daltons.

[0012] Preferably, the molecular weight of the high molecular weight zinc hyaluronate is ≥ 1 million Daltons.

[0013] Preferably, the molecular weight of high molecular weight zinc hyaluronate is 1.5 to 2 million Daltons.

[0014] The present invention does not impose any particular limitation on the preparation method of zinc hyaluronate, and the hyaluronate may be commercially available or homemade. The homemade method may be as follows: sodium hyaluronate is added to water, stirred to dissolve, an aqueous zinc chloride solution is added, stirred, filtered using a dialysis bag, and freeze-dried to obtain zinc hyaluronate.

[0015] Preferably, the mass ratio of the low molecular weight zinc hyaluronate, the high molecular weight zinc hyaluronate and the synergistic component is (15-20): (8-10): (2-5).

[0016] Preferably, the composition includes the following components in percentage by mass: 1.5%-2% low molecular weight zinc hyaluronate, 0.8%-1.0% high molecular weight zinc hyaluronate, 0.2%-0.5% synergistic component, 3.0%-5.0% glycerol, 1.0%-3.0% propylene glycol, 0.08%-0.24% α-glucan oligosaccharide, 0.3%-0.6% serine, 0.10%-0.17% water-soluble polymer, and water supplementation to 100%.

[0017] Preferably, the synergistic component is prepared by mixing Centella asiatica, Calendula officinalis, Turmeric, Rosemary and Chamomile and then fermenting them using a bacterial agent.

[0018] Preferably, the mass ratio of Centella asiatica, Calendula officinalis, Curcuma longa, Rosemary and Chamomile is (14-18): (5-10): (3-8): (7-12): (1-4).

[0019] Preferably, the bacterial agent includes Lactobacillus gasseri, Acetobacter pasteurianus and Lactobacillus plantarum.

[0020] Preferably, the bacterial agent comprises Lactobacillus gasseri, Acetobacter pasteurianus and Lactobacillus plantarum in a mass ratio of (1.3-1.6):1:(0.3-0.5).

[0021] Preferably, the preparation method of the synergistic component comprises the following steps:

[0022] (1) mixing Centella asiatica, Calendula officinalis, turmeric, rosemary, and chamomile and then grinding them to obtain a mixture;

[0023] (2) uniformly mixing the mixture and water, adding a microbial agent in an amount of 0.2-0.4% of the water mass, and aerobically fermenting at 30-40° C. for 3-5 days. After the fermentation is completed, sterilizing is performed to obtain a fermentation liquid;

[0024] (3) Filter the fermentation broth through a filter membrane, concentrate the filtrate, and spray-dry to obtain the synergistic component.

[0025] Preferably, the water-soluble polymer is selected from at least one of Span 20, carbomer, hydroxyethyl cellulose and xanthan gum.

[0026] A second aspect of the present invention provides a method for preparing a wound repair spray containing zinc hyaluronate, comprising the following steps: mixing a synergistic component and water, adding low molecular weight zinc hyaluronate and high molecular weight zinc hyaluronate, stirring evenly, sequentially adding serine, α-glucan oligosaccharide, glycerol, propylene glycol and a water-soluble polymer, stirring evenly, and obtaining a wound repair spray containing zinc hyaluronate.

[0027] Preferably, the preparation method further comprises stirring evenly, packaging in containers, sealing, and pressurizing.

[0028] A third aspect of the present invention provides a use of a wound repair spray containing zinc hyaluronate in the preparation of a drug for treating pressure injuries.

[0029] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0030] 1. The present invention provides a wound repair spray containing zinc hyaluronate. Through the compounding of multiple components, it can provide a microenvironment conducive to healing for pressure injury wounds. Zinc hyaluronate is compounded with other components to work synergistically, thereby accelerating the tissue repair process.

[0031] 2. The hyaluronate zinc spray described in the present invention can be used in combination with negative pressure sealed drainage technology, showing a synergistic effect in clinical applications. The present invention has significant application value in the treatment of pressure injuries, and is particularly suitable for alkaline wound environments where there is a risk of infection or infection has already occurred, and has good prospects for clinical promotion. DETAILED DESCRIPTION

[0032] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0033] The raw materials used in the following examples of the present invention are all commercially available commodities:

[0034] The hyaluronate zinc of different molecular weights used were all from Huaxi Biotechnology.

[0035] The bacterial strains used were all commercially available products purchased from Beijing Zhongke Quality Inspection Biotechnology Co., Ltd.: Lactobacillus gasseri (strain number: ZKCC12372), Acetobacter pasteurianus (strain number: ZKCC12083), Lactobacillus plantarum (strain number: ZKCC16010), Aspergillus oryzae (strain number: ZKCC15260), Candida utilis (strain number: ZKCC12620), Saccharomyces cerevisiae (strain number: ZKCC15672), Aspergillus niger (strain number: ZKCC153712), Bacillus subtilis (strain number: ZKCC153891), and Bacillus licheniformis (strain number: ZKCC25630).

[0036] The water-soluble polymer used in the following examples and comparative examples is Span 20.

[0037] Example 1

[0038] This embodiment provides a wound repair spray containing zinc hyaluronate, comprising the following components in percentage by weight: 1.7% low molecular weight zinc hyaluronate, 0.9% high molecular weight zinc hyaluronate, 0.3% synergistic component, 4% glycerol, 2% propylene glycol, 0.12% α-glucan oligosaccharide, 0.5% serine, 0.13% water-soluble polymer, and 100% water.

[0039] The molecular weight of the low molecular weight zinc hyaluronate is 30,000 Daltons.

[0040] High molecular weight zinc hyaluronate has a molecular weight of 1.6 million Daltons.

[0041] The preparation method of the synergistic component comprises the following steps:

[0042] (1) Centella asiatica, marigold, turmeric, rosemary, and chamomile are mixed and crushed to less than 80 meshes to obtain a mixture; the mass ratio of the Centella asiatica, marigold, turmeric, rosemary, and chamomile is 16:8:5:11:3.

[0043] (2) The mixture and water were mixed uniformly at a material-liquid ratio of 1 g:6 mL, and a bacterial agent was added, wherein the bacterial agent included Lactobacillus gasseri, Acetobacter pasteurianus, and Lactobacillus plantarum in a mass ratio of 1.5:1:0.4; the amount of the bacterial agent added was 0.3% of the water mass, and the mixture was aerobically fermented at 35° C. for 4 days with an air ventilation volume of 0.5 cubic meter / (cubic meter*minute). After the fermentation was completed, the mixture was sterilized to obtain a fermentation liquid;

[0044] (3) The fermentation broth was filtered through a 0.45 μm filter membrane, the filtrate was concentrated, and spray-dried to obtain the synergistic component.

[0045] The preparation method of the wound repair spray containing zinc hyaluronate comprises the following steps: mixing a synergistic component and water, adding low-molecular-weight zinc hyaluronate and high-molecular-weight zinc hyaluronate, stirring evenly, sequentially adding serine, α-glucan oligosaccharide, glycerol, propylene glycol, and a water-soluble polymer, stirring evenly, and obtaining the wound repair spray containing zinc hyaluronate.

[0046] Example 2

[0047] This embodiment provides a wound repair spray containing zinc hyaluronate, comprising the following components in mass percentages: 1.5% low molecular weight zinc hyaluronate, 1.0% high molecular weight zinc hyaluronate, 0.2% synergistic component, 5.0% glycerol, 1.0% propylene glycol, 0.24% α-glucan oligosaccharide, 0.3% serine, 0.17% water-soluble polymer, and 100% water.

[0048] The molecular weight of the low molecular weight zinc hyaluronate is 10,000 Daltons.

[0049] High molecular weight zinc hyaluronate has a molecular weight of 2 million Daltons.

[0050] The preparation method of the synergistic component comprises the following steps:

[0051] (1) Centella asiatica, marigold, turmeric, rosemary, and chamomile are mixed and crushed to less than 80 meshes to obtain a mixture; the mass ratio of the Centella asiatica, marigold, turmeric, rosemary, and chamomile is 14:10:3:12:1.

[0052] (2) The mixture and water were mixed uniformly at a material-liquid ratio of 1 g:5 mL, and a bacterial agent was added, wherein the bacterial agent included Lactobacillus gasseri, Acetobacter pasteurianus, and Lactobacillus plantarum in a mass ratio of 1.3:1:0.5; the amount of the bacterial agent added was 0.2% of the mass of the water, and the mixture was aerobically fermented at 35° C. for 5 days with an air ventilation volume of 0.4 cubic meters / (cubic meters*minutes). After the fermentation was completed, the mixture was sterilized to obtain a fermentation liquid;

[0053] (3) The fermentation broth was filtered through a 0.45 μm filter membrane, the filtrate was concentrated, and spray-dried to obtain the synergistic component.

[0054] The preparation method of the wound repair spray containing zinc hyaluronate comprises the following steps: mixing a synergistic component and water, adding low-molecular-weight zinc hyaluronate and high-molecular-weight zinc hyaluronate, stirring evenly, sequentially adding serine, α-glucan oligosaccharide, glycerol, propylene glycol, and a water-soluble polymer, stirring evenly, and obtaining the wound repair spray containing zinc hyaluronate.

[0055] Example 3

[0056] This embodiment provides a wound repair spray containing zinc hyaluronate, comprising the following components in mass percentages: 2% low molecular weight zinc hyaluronate, 0.8% high molecular weight zinc hyaluronate, 0.5% synergistic component, 3.0% glycerol, 3.0% propylene glycol, 0.08% α-glucan oligosaccharide, 0.6% serine, 0.10% water-soluble polymer, and 100% water.

[0057] The molecular weight of the low molecular weight zinc hyaluronate is 50,000 Daltons.

[0058] High molecular weight zinc hyaluronate has a molecular weight of 1.5 million Daltons.

[0059] The preparation method of the synergistic component comprises the following steps:

[0060] (1) Centella asiatica, marigold, turmeric, rosemary, and chamomile are mixed and crushed to less than 80 meshes to obtain a mixture; the mass ratio of the Centella asiatica, marigold, turmeric, rosemary, and chamomile is 18:5:8:7:4.

[0061] (2) The mixture and water were mixed uniformly at a material-liquid ratio of 1 g:8 mL, and a bacterial agent was added, wherein the bacterial agent included Lactobacillus gasseri, Acetobacter pasteurianus, and Lactobacillus plantarum in a mass ratio of 1.6:1:0.3; the amount of the bacterial agent added was 0.4% of the mass of the water, and the mixture was aerobically fermented at 35° C. for 3 days with an air ventilation volume of 0.6 cubic meters / (cubic meters*minutes). After the fermentation was completed, the mixture was sterilized to obtain a fermentation liquid;

[0062] (3) The fermentation broth was filtered through a 0.45 μm filter membrane, the filtrate was concentrated, and spray-dried to obtain the synergistic component.

[0063] The preparation method of the wound repair spray containing zinc hyaluronate comprises the following steps: mixing a synergistic component and water, adding low-molecular-weight zinc hyaluronate and high-molecular-weight zinc hyaluronate, stirring evenly, sequentially adding serine, α-glucan oligosaccharide, glycerol, propylene glycol, and a water-soluble polymer, stirring evenly, and obtaining the wound repair spray containing zinc hyaluronate.

[0064] Example 4

[0065] The difference between this embodiment and embodiment 1 is that the mass percentages of the components are different.

[0066] A wound repair spray containing zinc hyaluronate, characterized by comprising the following components in percentage by mass: 2.5% low molecular weight zinc hyaluronate, 0.2% high molecular weight zinc hyaluronate, 0.7% synergistic component, 1% glycerol, 5.0% propylene glycol, 0.3% α-glucan oligosaccharide, 0.8% serine, 0.6% water-soluble polymer, and 100% water.

[0067] Example 5

[0068] The difference between this embodiment and embodiment 1 is that the mass percentages of low molecular weight zinc hyaluronate, high molecular weight zinc hyaluronate and synergistic components are different, but the total amount remains unchanged.

[0069] A wound repair spray containing zinc hyaluronate comprises the following components in percentage by mass: 1.0% low molecular weight zinc hyaluronate, 1.2% high molecular weight zinc hyaluronate, 0.7% synergistic component, 4% glycerol, 2% propylene glycol, 0.12% α-glucan oligosaccharide, 0.5% serine, 0.13% water-soluble polymer, and 100% water.

[0070] Example 6

[0071] The difference between this comparative example and Example 1 is that the mass percentages of low molecular weight zinc hyaluronate, high molecular weight zinc hyaluronate and the synergistic component are different, but the total amount remains unchanged.

[0072] A wound repair spray containing zinc hyaluronate comprises the following components in percentage by mass: 2.2% low molecular weight zinc hyaluronate, 0.6% high molecular weight zinc hyaluronate, 0.1% synergistic component, 4% glycerol, 2% propylene glycol, 0.12% α-glucan oligosaccharide, 0.5% serine, 0.13% water-soluble polymer, and 100% water.

[0073] Comparative Example 1

[0074] The difference between this comparative example and Example 1 is that high molecular weight zinc hyaluronate is not added.

[0075] A wound repair spray containing zinc hyaluronate comprises the following components in percentage by weight: 2.6% low-molecular-weight zinc hyaluronate, 0.3% synergistic component, 4% glycerol, 2% propylene glycol, 0.12% α-glucan oligosaccharide, 0.5% serine, 0.13% water-soluble polymer, and 100% water. The low-molecular-weight zinc hyaluronate has a molecular weight of 30,000 Daltons.

[0076] Comparative Example 2

[0077] The difference between this comparative example and Example 1 is that low molecular weight zinc hyaluronate is not added.

[0078] A wound repair spray containing zinc hyaluronate comprises the following components in percentage by weight: 2.6% high molecular weight zinc hyaluronate, 0.3% synergistic component, 4% glycerol, 2% propylene glycol, 0.12% α-glucan oligosaccharide, 0.5% serine, 0.13% water-soluble polymer, and 100% water. The high molecular weight zinc hyaluronate has a molecular weight of 1.6 million daltons.

[0079] Comparative Example 3

[0080] The difference between this comparative example and Example 1 is that no synergistic component is added, and the total amount of the synergistic component, low molecular weight zinc hyaluronate, and high molecular weight zinc hyaluronate remains unchanged.

[0081] A wound repair spray containing zinc hyaluronate comprises the following components in percentage by mass: 1.9% low molecular weight zinc hyaluronate, 1.0% high molecular weight zinc hyaluronate, 4% glycerol, 2% propylene glycol, 0.12% α-glucan oligosaccharide, 0.5% serine, 0.13% water-soluble polymer, and 100% water.

[0082] Comparative Example 4

[0083] The difference between this comparative example and Example 1 is that the mass ratio of the Centella asiatica, marigold, turmeric, rosemary and chamomile is 8:3:11:5:16.

[0084] Comparative Example 5

[0085] The difference between this comparative example and Example 1 is that the bacterial agent comprises Aspergillus oryzae, Candida utilis and Saccharomyces cerevisiae in a mass ratio of 1.6:1:0.3.

[0086] Comparative Example 6

[0087] The difference between this comparative example and Example 1 is that the bacterial agent includes Aspergillus niger, Bacillus subtilis and Bacillus licheniformis in a mass ratio of 1.6:1:0.3.

[0088] Comparative Example 7

[0089] This comparative example is a wound repair spray prepared with reference to Example 1 in patent CN112773816B, a wound repair spray and its preparation method and application.

[0090] Performance Testing

[0091] 1. This study used SPF-grade male C57BL / 6J mice, 7-8 weeks old and weighing an average of 22 g. The environmental parameters were: temperature (22 ± 1) °C, relative humidity (50 ± 5)%, and a 12-h light-dark cycle. Food and water were provided ad libitum during the experiment.

[0092] A hairless observation area was established on the back skin of the mice. The exposed skin was lifted vertically and circular magnets (8 mm in diameter, N52 grade) were symmetrically attached on both sides. A constant pressure of 45 kPa was applied for 6 hours per day for 5 consecutive cycles. Obvious pressure injury wounds were visible on the back skin, accompanied by exudate, indicating that the model was successful.

[0093] The mice after modeling were randomly divided into experimental group and control group, with 6 mice in each group.

[0094] Control group: The wound was covered with sterile gauze and fixed with a sterile elastic bandage. The gauze was changed every 24 hours, and the intervention lasted for 7 days.

[0095] Experimental group: The wound surface was evenly sprayed with the wound repair spray prepared in Examples 1-6 and Comparative Examples 1-7 (about 0.10 mL / cm2) to form a moist, drip-free film layer. The wound surface was covered with sterile gauze, and the outer layer was fixed with a sterile elastic bandage. The spray and gauze were replaced synchronously every 24 hours for 7 consecutive days.

[0096] (1) The wound healing rate of mice after 7 days was calculated as follows: (initial wound area - wound area after 7 days) / initial wound area × 100%.

[0097] (2) Calculate the time required from the start of surgery to the complete coverage of the wound by the epidermis (average healing time).

[0098] (3) Measure the pH value of wound exudate before and after intervention.

[0099] The results are shown in Table 1.

[0100] Table 1 Healing of pressure injury wounds in mice

[0101]

[0102]

[0103] As shown in Table 1, the wound repair agents prepared in Examples 1-6 significantly improved the healing rate of pressure injuries in mice and shortened the healing time. However, the results show that the healing time of Examples 5-6 was significantly longer than that of Examples 1-3, indicating that the composition of the wound repair spray has a significant impact on the subsequent healing of the wound. Furthermore, the results of the Examples and Comparative Example 3 show that the combination of zinc hyaluronate and the synergistic component has a synergistic effect, and when added together, it can significantly improve the wound treatment effect.

[0104] Since the alkaline environment of pressure injury wounds is associated with the occurrence of infection, the pH value of the wound surface will continue to rise in the event of infection, showing an alkaline trend, accompanied by a large number of bacterial colonization. The results show that the wound repair agent prepared by the present invention can effectively improve the pH value of the wound microenvironment, creating an environment more conducive to tissue repair, thereby accelerating the healing process.

[0105] 2. The research subjects were patients with infectious stage 3 and 4 pressure injuries who were treated in Zhejiang Xiaoshan Hospital between February 2022 and February 2024. All patients had no history of allergy to the wound repair spray of Example 1; the patients' pressure ulcer diagnosis met the international Npuap / Epuap pressure ulcer classification system standards. There was no statistically significant difference in general information between the experimental group and the control group (P>0.05), and the two groups were comparable.

[0106] A total of 80 patients who met the criteria were enrolled, with 40 in the control group and 40 in the experimental group. One patient in the control group could no longer be observed due to a change in condition, and two patients withdrew from clinical observation for personal reasons. Two patients in the experimental group were transferred to other departments due to a change in condition.

[0107] The experimental group (n=38) was treated with the wound repair spray prepared in Example 1 combined with negative pressure sealing drainage technology. There were 23 males and 15 females with an average age of 65.50±4.13 years. All injuries were located in the sacrum and coccyx. The pressure ulcer stages were: 18 cases at stage 3 and 20 cases at stage 4.

[0108] The control group (n=37) was treated with negative pressure sealing drainage. There were 23 males and 14 females with a mean age of 63.50±5.83 years. All lesions were located in the sacrum and coccyx. The pressure ulcer stages were: stage 3 in 19 cases and stage 4 in 18 cases.

[0109] Treatment:

[0110] The control group received standard negative pressure sealing drainage technology, with the negative pressure set at 90-100 mmHg. The treatment frequency was the same as the experimental group, and treatment lasted for 8 consecutive weeks.

[0111] Experimental group: In addition to the treatment of the control group, the wound repair spray of Example 1 was used. Specific procedures: After the wound was disinfected, the wound repair spray was evenly sprayed on the entire wound surface. The spray amount was enough to cover the wound to form a thin layer (about 0.10mL / cm2). The wound was allowed to stand for 5 minutes, and then VSD treatment was performed according to the method described for the control group. The wound repair spray was sprayed daily for the first week and the VSD dressing was replaced at the same time. The wound repair spray was sprayed every 3 days for the second to fourth weeks and the VSD dressing was replaced at the same time. The wound repair spray was sprayed every 5 days thereafter and the VSD dressing was replaced at the same time. The treatment was continued for 8 weeks.

[0112] The wound healing rates of the patients were calculated after 8 weeks, and the results are shown in Table 2.

[0113] Table 2 Wound healing rate (%) and wound healing time

[0114] 4 weeks 8 weeks Wound healing time / month Experimental group 69.15±9.32 88.47±8.57 3.02±0.47 control group 52.63±7.04 71.56±7.61 3.71±0.63

[0115] The results show that the wound healing rates of the experimental group after 4 and 8 weeks of treatment were higher than those of the control group, and the wound healing time was significantly shortened. The wound repair spray prepared by the present invention can achieve full-process healing efficiency by synergizing the mechanical drainage and microcirculation improvement functions of the VSD.

[0116] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A wound repair spray containing zinc hyaluronate, characterized in that: The invention comprises the following components in percentage by mass: 0.05%-5% low molecular weight zinc hyaluronate, 0.05%-5% high molecular weight zinc hyaluronate, 0.01%-1% synergistic component, 2.0%-8.0% glycerol, 1.0%-5.0% propylene glycol, 0.05%-0.5% α-glucan oligosaccharide, 0.1%-1.0% serine, 0.05%-0.3% water-soluble polymer, and water supplementation to 100%.

2. The wound repair spray containing zinc hyaluronate according to claim 1, characterized in that The molecular weight of the low molecular weight zinc hyaluronate is ≤100,000 Daltons.

3. The wound repair spray containing zinc hyaluronate according to claim 2, characterized in that The molecular weight of the high molecular weight zinc hyaluronate is ≥1 million Daltons.

4. The wound repair spray containing zinc hyaluronate according to claim 3, characterized in that: The synergistic component is prepared by mixing centella asiatica, marigold, turmeric, rosemary and chamomile and then fermenting the mixture using a bacterial agent.

5. The wound repair spray containing zinc hyaluronate according to claim 4, characterized in that The mass ratio of the Centella asiatica, marigold, turmeric, rosemary and chamomile is (14-18): (5-10): (3-8): (7-12): (1-4).

6. The wound repair spray containing zinc hyaluronate according to claim 5, characterized in that: The bacterial agents include Lactobacillus gasseri, Acetobacter pasteurianus and Lactobacillus plantarum.

7. The wound repair spray containing zinc hyaluronate according to claim 5, characterized in that: The mass ratio of the low molecular weight zinc hyaluronate, the high molecular weight zinc hyaluronate and the synergistic component is (15-20): (8-10): (2-5).

8. The wound repair spray containing zinc hyaluronate according to claim 1, characterized in that The water-soluble polymer is selected from at least one of Span 20, carbomer, hydroxyethyl cellulose and xanthan gum.

9. A method for preparing the wound repair spray containing zinc hyaluronate according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: mixing the synergistic component and water, adding low molecular weight zinc hyaluronate and high molecular weight zinc hyaluronate, stirring evenly, and then sequentially adding serine, α-glucan oligosaccharide, glycerol, propylene glycol and water-soluble polymer, stirring evenly to obtain a wound repair spray containing zinc hyaluronate.

10. Use of the wound repair spray containing zinc hyaluronate according to any one of claims 1 to 8 in the preparation of a medicine for treating pressure injuries.

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