Method for full-automatic lossless separation and enrichment of rare cells

By combining microfluidic chips and cell filtration membranes, using bottom-up flow direction and pressure sensor monitoring, the problems of pore blockage and cell damage during cell separation were solved, achieving efficient and simplified rare cell separation and enrichment.

CN120607951APending Publication Date: 2025-09-09INST OF PHYSICS HENAN ACAD OF SCI +1
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Patent Information

Application Number
CN202510558417.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-29
Publication Date
2025-09-09

AI Technical Summary

Technical Problem

Existing cell separation methods are prone to cell clogging and pressure differences affecting cell structure during the sorting process, resulting in poor sorting efficiency and sensitivity, complex operations and high costs.

Method used

By combining microfluidic chips and cell filtration membranes, the flow direction is adjusted from bottom to top, the fluid dynamics conditions are precisely controlled, and integrated pressure sensors are used for real-time monitoring and to prevent blockage, thereby achieving efficient separation and enrichment of rare cells.

Benefits of technology

It improves separation efficiency and accuracy, reduces cell damage, simplifies operation procedures, and reduces costs. It is suitable for a variety of cell separations, especially the separation and enrichment of circulating tumor cells.

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Abstract

The invention relates to a method for full-automatic lossless separation and enrichment of rare cells, which utilizes a micro-flow device, a pressure sensor and a full-automatic control system from bottom to top, and the device can realize efficient separation and enrichment of rare cells without pre-treating thick cell liquid. By accurately controlling fluid dynamic conditions and optimizing the cell flow velocity and pressure, the method can maintain the cell activity and integrity and improve the capture rate and purity of rare cells at the same time. The design of the device allows separated and enriched rare cells to be directly collected into a collection bottle containing a cell culture medium. By means of the design, it is ensured that cells are in a proper growth environment, follow-up cell culture, downstream analysis and treatment are facilitated, and therefore the experiment efficiency and the result reliability are improved. According to the method, the traditional process is simplified, the sample treatment time is shortened, and an efficient and reliable solution is provided for cytological research and clinical diagnosis.
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