Single molecule in-situ recognition and detection method based on intermolecular hydrogen bonds

By modifying gold nanoelectrodes and bridging molecules based on intermolecular hydrogen bonds, combined with electrochemical regulation and SERS technology, the shortcomings of single-molecule detection technology in specificity and dynamic response dimensions are solved, and high-sensitivity, multi-dimensional detection of biological molecules is achieved, especially in the fields of biological molecule detection and high-throughput screening, which has broad application prospects.

CN120629112APending Publication Date: 2025-09-12HUBEI UNIV OF AUTOMOTIVE TECH
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Patent Information

Application Number
CN202510949150.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-10
Publication Date
2025-09-12

AI Technical Summary

Technical Problem

Existing single-molecule detection technologies have deficiencies in specific recognition capabilities, dynamic response dimensions, and detection flux, making it difficult to achieve highly sensitive, multi-dimensional detection of trace biological molecules in complex biological systems, especially the low detection sensitivity of hydrogen bond networks.

Method used

A method based on intermolecular hydrogen bonds is adopted. By preparing gold nanoelectrodes and modifying bridging molecules, the nanogap is controlled by electrochemistry, and the hydrogen bond interactions are monitored in real time in combination with SERS technology to achieve high sensitivity and multi-dimensional detection of target molecules.

Benefits of technology

It achieves high-precision recognition and electrochemical regulation of single biological molecules, significantly improves signal intensity and detection response, has sub-nanometer spatial resolution, is suitable for qualitative and quantitative analysis of a variety of biological molecules, and has good versatility and expansion potential.

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Abstract

The invention relates to a monomolecular in-situ recognition and detection method based on intermolecular hydrogen bonds, and belongs to the technical field of monomolecular detection.The method comprises the steps that a gold nanoelectrode is prepared through an electrochemical corrosion method, then a bridging molecule solution with sulfydryl is combined with the surface of the gold nanoelectrode, meanwhile, gold nanoparticles are mixed with the bridging molecule solution, and the gold nanoparticles are obtained; the method comprises the following steps: modifying a gold nano-electrode, placing the modified gold nano-electrode in a modified gold nano-particle solution, adding a target molecule solution into the gold nano-particle solution, forming molecular knots by virtue of an electrostatic adsorption effect between gold nano-particles and the gold nano-electrode, dynamically adjusting the molecular knots by virtue of electrochemical regulation, and finally monitoring in real time by virtue of an SERS (Surface Enhanced Raman Scattering) signal. And obtaining identification information of the target molecule. Therefore, the method has the advantages of high identification precision, high sensitivity and real-time dynamic monitoring.
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