Method for constructing anxiety mouse model based on terahertz waves

Abstract

The invention discloses a method for constructing an anxiety mouse model based on terahertz waves, relates to the technical field of anxiety animal models, and solves the problems that an anxiety animal model in the prior art depends on a behavioral test and has limitation in the aspect of accurate evaluation of a neurobiological mechanism; the related research mostly focuses on the local effect of the terahertz wave on the cells or tissues, and the system evaluation on the overall behavioristics and neurobiology influence is lacked. The method comprises the following steps: randomly distributing a plurality of mice into two groups; carrying out adaptive feeding and inverted photoperiod feeding on the two groups of mice; one of the two groups of fed mice is used as an experimental group, and the other group is used as a control group; carrying out irradiation treatment on the mice of the experimental group and the control group by constructing a platform for irradiating the mice with terahertz waves; and respectively carrying out behavioral detection of an open field experiment, an elevated cross labyrinth experiment, a three-room social experiment and a black-and-white box experiment on the mice in the control group and the experimental group after irradiation treatment, so as to solve the technical problems.

Description

Technical Field

[0001] The present invention belongs to the field of anxiety animal models, and specifically is a method for constructing an anxiety mouse model based on terahertz waves. Background Art

[0002] Anxiety disorder is a common psychological disorder with a complex pathogenesis involving the interaction of multiple systems, including neurotransmitters, neuroendocrine, and neuroimmune systems. To better understand the pathophysiological mechanisms of anxiety disorders and develop effective treatments, it is crucial to construct reliable and reproducible animal models of anxiety. Currently, commonly used animal models of anxiety include the elevated plus maze, open field box, and social avoidance. These models rely on behavioral tests and, while they can simulate human anxiety symptoms to a certain extent, have limitations in accurately assessing neurobiological mechanisms.

[0003] In recent years, terahertz technology has become a hot topic in biomedical research due to its unique biological effects and safety. Terahertz waves, with a frequency band between microwaves and infrared, can penetrate biological tissue and affect the structure and function of cells and molecules. Studies have shown that terahertz waves can induce anxiety-like behavioral and physiological responses under specific conditions by modulating neuronal activity, influencing neurotransmitter release, and altering metabolic states in brain tissue. Using terahertz waves to construct animal models of anxiety not only improves the accuracy and controllability of the models but also provides new tools and methods for studying the neural mechanisms underlying anxiety disorders.

[0004] However, there is currently no systematic research on methods for constructing anxiety mouse models based on terahertz waves. Existing related studies have mostly focused on the local effects of terahertz waves on cells or tissues, lacking a systematic assessment of their overall behavioral and neurobiological impacts. Therefore, the present invention proposes a method for constructing an anxiety mouse model based on terahertz waves to address the technical issues that commonly used anxiety animal models in the prior art rely on behavioral testing, which is limited in accurately assessing neurobiological mechanisms. Furthermore, related studies have mostly focused on the local effects of terahertz waves on cells or tissues, lacking a systematic assessment of their overall behavioral and neurobiological impacts. Summary of the Invention

[0005] The present invention aims to solve at least one of the technical problems existing in the prior art; to this end, the present invention proposes a method for constructing an anxiety mouse model based on terahertz waves, which is used to solve the technical problems that the anxiety animal models commonly used in the prior art rely on behavioral tests, have limitations in accurately evaluating neurobiological mechanisms, and related research mostly focuses on the local effects of terahertz waves on cells or tissues, lacking a systematic evaluation of the overall behavioral and neurobiological effects.

[0006] To achieve the above objectives, the first aspect of the present invention provides a method for constructing an anxiety mouse model based on terahertz waves, comprising: Several mice were randomly assigned into two groups; Two groups of mice were subjected to adaptive housing and inverted light cycle housing; One of the two groups of mice after feeding was used as the experimental group, and the other was used as the control group; A terahertz wave irradiation platform for mice was constructed to irradiate mice in the experimental and control groups; The irradiated mice in the control and experimental groups were subjected to behavioral tests including open field test, elevated plus maze test, three-chamber social test and black-white box test.

[0007] In combination with the first aspect above, in a possible implementation, the adaptive breeding includes: Both groups of mice were placed in an SPF-grade experimental animal room; The mice are raised for a certain period of time according to preset environmental conditions; wherein the preset environmental conditions include: the optimal survival temperature and optimal survival humidity of the mice.

[0008] It should be noted that both groups of mice were 6-8 weeks old and in puberty; SPF-grade experimental animals are animals that are free of specific pathogens and do not carry major potential infections or pathogens that cause conditional diseases or interfere with scientific experiments. SPF-grade experimental animal rooms are places that can provide experimental animals that meet this level of protection in a barrier system or isolation system. SPF-grade experimental animal rooms implement strict microbial control, and the experimental environment strictly controls the entry and exit of personnel, objects and air. The stable initial environment of adaptive rearing can reduce the nonspecific anxiety of mice caused by environmental changes and ensure that the subsequent experimental results are caused by terahertz irradiation.

[0009] In combination with the first aspect above, in a possible implementation, the inverted photoperiod breeding includes: The preset environmental conditions of the two groups of mice were kept unchanged, and the two groups of mice after adaptive feeding were raised under a 12-hour inverted light cycle for a certain period of time.

[0010] It should be noted that the 12-hour inverted light cycle refers to a lighting setting that reverses the natural circadian rhythm, for example, the light period: 09:00-21:00 (a total of 12 hours of light); the dark period: 21:00-09:00 (a total of 12 hours of darkness); Inverting the light cycle can artificially cause "biological clock imbalance" in mice, similar to shift work and insomnia in humans. Circadian rhythm disorders are correlated with the occurrence of anxiety disorders and can serve as an auxiliary stress factor for terahertz irradiation to enhance the anxiety-inducing effect. The control group and the experimental group were raised under the same abnormal light cycle to eliminate the interference of the natural circadian rhythm on the experimental results and ensure that terahertz irradiation is the core variable.

[0011] In combination with the first aspect above, in a possible implementation, constructing a terahertz wave irradiation platform for mice to irradiate mice in the experimental group and the control group includes: A1: The terahertz wave irradiation platform for mice in the experimental group performs fixed-point terahertz irradiation on the head according to the preset irradiation time; A2: Stop the terahertz source and perform the same operation as step A1 on the control group mice.

[0012] It should be noted that when performing fixed-point head terahertz irradiation, the mouse was fixed in a holder so that the terahertz wave was focused on the head; The preset irradiation time is the irradiation time and duration set by those skilled in the art based on actual experience, such as fixed-point head terahertz irradiation for 15 minutes every day for 5 consecutive days.

[0013] By stimulating the mouse's head with terahertz waves, neurophysiological changes are induced, resulting in anxiety-like behaviors. Terahertz waves can penetrate brain tissue, regulate neuronal electrical activity, the release of neurotransmitters (such as serotonin and dopamine), and brain metabolic state, thereby inducing anxiety-related behaviors.

[0014] In combination with the first aspect above, in one possible implementation, constructing a platform for irradiating mice with terahertz waves includes: The terahertz wave irradiation platform for mice is composed of a terahertz source, a plane mirror, a polyethylene lens, a mouse holder, a bracket and a lifting platform, specifically: Place the terahertz emission source on a lifting platform; The plane mirror is fixed on the bracket at an angle of 45°, with the mirror facing downward; The polyethylene lens is fixed below the plane mirror of the bracket and placed horizontally so that the center of the lens and the center of the plane mirror are on the same vertical line; Adjust the height of the lifting platform so that the waveguide port of the terahertz emission source is aligned with the center of the plane mirror; Place the mouse holder under the center of the lens.

[0015] In combination with the first aspect above, in one possible implementation, the irradiated control group and experimental group mice are subjected to behavioral tests including an open field test, an elevated plus maze test, a three-chamber social interaction test, and a black-and-white box test, respectively, including: The mice in the control group and the experimental group were subjected to the open field test, elevated plus maze test, three-chamber social test and black-white box test, respectively, and the corresponding indicator data of each experiment were recorded; among them, the indicator data included: open field test indicators, elevated plus maze test indicators, three-chamber social test indicators and black-white box test indicators; open field test indicators included: the time the mouse spent in the center of the open field and the distance moved in the center of the open field; elevated plus maze test indicators included: the number and time the mouse entered the open arm; three-chamber social test indicators included: the number and time the mouse sniffed in the left cage; black-white box test indicators included: the distance and time the mouse entered the white box; Calculate the mean and standard deviation of the indicator data corresponding to the experimental group and the control group in each experiment, and use the independent sample t test to calculate the P value of the corresponding indicator of the experimental group and the control group; If the P value is less than or equal to the preset threshold, it means that the difference in indicator data between the experimental group and the control group is statistically significant, that is, terahertz irradiation successfully induces anxiety-like behavior in mice, and the anxiety mouse model is successfully established.

[0016] It should be noted that the P value (Probability value) is a key statistic in hypothesis testing, which is used to measure the probability that "the difference between the experimental group and the control group is caused by random error"; if the P value is less than or equal to the preset threshold, it indicates that the probability of the difference being caused by random error is extremely small, and the difference can be considered statistically significant (that is, terahertz irradiation is likely to have induced anxiety behavior); at the same time, the P value provides quantitative statistical evidence for the effectiveness of the model, avoiding subjective judgment.

[0017] In combination with the first aspect above, in a possible implementation, the method for obtaining open field test indicators includes: The mouse was placed in the center of the open field, and the timing began when the mouse entered the open field; The time the mouse spends in the center of the open field and the distance it moves in the center of the open field are recorded within the set time.

[0018] In combination with the first aspect above, in one possible implementation, the method for obtaining the elevated plus maze test index includes: It was set that the mouse's hind legs entered the open arm 5 cm once; The mouse was placed in the center of the plus maze with its head facing the open arm, and the timing began when the mouse entered the plus maze; The number and time of mice entering the open arms within the set time were recorded.

[0019] In conjunction with the first aspect above, in one possible implementation, the method for obtaining the three-chamber social experiment indicator includes: A sniff was counted as one time when the mouse's head was within 3 cm of the left cage and stayed there for at least 1 second; Place the stranger mouse in the cage of the left box, place the mouse in the middle box, open the sliding doors of the left and right boxes, and start timing from the moment the mouse enters the middle box; The number and duration of sniffing by the mouse in the left cage were recorded within the set time.

[0020] It should be noted that the stranger mice refer to healthy mice of the same strain and sex that have no history of social contact with the experimental and control group mice, and are used to stimulate the social behavior of the experimental mice.

[0021] In combination with the first aspect above, in a possible implementation, the method for obtaining the black-and-white box experiment indicator includes: Place the mouse in the small door between the light and dark boxes, and start counting from the moment the mouse enters the black and white box; The movement distance and time of the mouse entering the white box within the set time were recorded.

[0022] Compared with the prior art, the present invention has the following beneficial effects: In the existing technology, commonly used animal models of anxiety rely on behavioral tests, which have limitations in accurately evaluating neurobiological mechanisms. Related studies mostly focus on the local effects of terahertz waves on cells or tissues, and lack a systematic evaluation of the overall behavioral and neurobiological effects. At the same time, traditional models may involve drug injections or external blows, which may cause harm to experimental animals. The present invention uses terahertz waves to provide external environmental stimulation to mice, avoiding the effects of drugs, causing no harm to mice, and is more humane. By adaptively feeding mice, grouping and inverting the light cycle, and terahertz fixed-point irradiation, combined with behavioral tests such as open field experiments, elevated plus maze experiments, three-chamber social experiments, and black-and-white box experiments, the effects of terahertz waves on the overall behavior and neurobiology of mice are systematically evaluated, and an anxiety mouse model is successfully constructed, providing more effective means and technical support for the study of neuropsychiatric diseases such as anxiety. At the same time, the model has good reliability and repeatability, and provides solutions and experimental basis for the later promotion of the clinical application of terahertz neuromodulation technology and the treatment of neurodegenerative diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1 Schematic diagram of the method steps of an embodiment of the present invention; Figure 2Schematic diagram of a terahertz wave irradiation platform for mice according to an embodiment of the present invention. DETAILED DESCRIPTION

[0025] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] See also Figure 1 The first embodiment of the present invention provides a method for constructing an anxiety mouse model based on terahertz waves, comprising: Several mice were randomly assigned into two groups; Two groups of mice were subjected to adaptive housing and inverted light cycle housing; One of the two groups of mice after feeding was used as the experimental group, and the other was used as the control group; A terahertz wave irradiation platform for mice was constructed to irradiate mice in the experimental and control groups; The irradiated mice in the control and experimental groups were subjected to behavioral tests including open field test, elevated plus maze test, three-chamber social test and black-white box test.

[0027] Adaptive husbandry, including: Both groups of mice were placed in an SPF-grade experimental animal room; The mice are raised for a certain period of time according to preset environmental conditions; wherein the preset environmental conditions include: the optimal survival temperature and optimal survival humidity of the mice.

[0028] Inverted photoperiod rearing, including: The preset environmental conditions of the two groups of mice were kept unchanged, and the two groups of mice after adaptive feeding were raised under a 12-hour inverted light cycle for a certain period of time.

[0029] A terahertz wave irradiation platform for mice was constructed to irradiate mice in the experimental and control groups, including: A1: The terahertz wave irradiation platform for mice in the experimental group performs fixed-point terahertz irradiation on the head according to the preset irradiation time; A2: Stop the terahertz source and perform the same operation as step A1 on the control group mice.

[0030] See Figure 2 , building a terahertz wave irradiation platform for mice, including: The terahertz wave irradiation platform for mice is composed of a terahertz source, a plane mirror, a polyethylene lens, a mouse holder, a bracket and a lifting platform, specifically: Place the terahertz emission source on a lifting platform; The plane mirror is fixed on the bracket at an angle of 45°, with the mirror facing downward; The polyethylene lens is fixed below the plane mirror of the bracket and placed horizontally so that the center of the lens and the center of the plane mirror are on the same vertical line; Adjust the height of the lifting platform so that the waveguide port of the terahertz emission source is aligned with the center of the plane mirror; Place the mouse holder under the center of the lens.

[0031] Example 1: Follow the steps below to build a terahertz wave irradiation platform for mice: Required instruments: Prepare a terahertz emission source with a frequency of 0.22 THz, a plane mirror, a polyethylene lens, a bracket, a mouse holder, and a lifting platform; Platform setup: Place the terahertz emission source on the lifting platform; fix the plane mirror on the bracket at a 45° angle, with the mirror facing downward; fix the polyethylene lens below the plane mirror on the bracket, place it horizontally, and keep the center of the lens and the center of the plane mirror on the same vertical line; adjust the height of the lifting platform so that the waveguide port of the terahertz emission source is in the center of the plane mirror; place the mouse holder below the center of the lens; Parameter adjustment: Turn on the terahertz emission source, adjust the height of the lifting platform, and the distance between the plane mirror and the lens on the bracket, so that the power density of the terahertz wave emitted by the terahertz source reaching the mouse holder is 200mW / cm 2 .

[0032] The irradiated mice in the control and experimental groups were subjected to behavioral tests including open field test, elevated plus maze test, three-chamber social test and black-white box test, including: The mice in the control group and the experimental group were subjected to the open field test, elevated plus maze test, three-chamber social test and black-white box test, respectively, and the corresponding indicator data of each experiment were recorded; among them, the indicator data included: open field test indicators, elevated plus maze test indicators, three-chamber social test indicators and black-white box test indicators; open field test indicators included: the time the mouse spent in the center of the open field and the distance moved in the center of the open field; elevated plus maze test indicators included: the number and time the mouse entered the open arm; three-chamber social test indicators included: the number and time the mouse sniffed in the left cage; black-white box test indicators included: the distance and time the mouse entered the white box; Calculate the mean and standard deviation of the corresponding indicator data of the experimental group and the control group in each experiment, and use the independent sample t test to calculate the P value of the corresponding indicator of the experimental group and the control group; If the P value is less than or equal to the preset threshold, it means that the difference in indicator data between the experimental group and the control group is statistically significant, that is, terahertz irradiation successfully induces anxiety-like behavior in mice, and the anxiety mouse model is successfully established.

[0033] Methods for obtaining open field test indicators include: The mouse was placed in the center of the open field, and the timing began when the mouse entered the open field; The time the mouse spends in the center of the open field and the distance it moves in the center of the open field are recorded within the set time.

[0034] Methods for obtaining indicators of the elevated plus maze experiment include: It was set that the mouse's hind legs entered the open arm 5 cm once; The mouse was placed in the center of the plus maze with its head facing the open arm, and the timing began when the mouse entered the plus maze; The number and time of mice entering the open arms within the set time were recorded.

[0035] The method for obtaining the three-chamber social experiment indicators includes: A sniff was counted as one time when the mouse's head was within 3 cm of the left cage and stayed there for at least 1 second; Place the stranger mouse in the cage of the left box, place the mouse in the middle box, open the sliding doors of the left and right boxes, and start timing from the moment the mouse enters the middle box; The number and duration of sniffing by the mouse in the left cage were recorded within the set time.

[0036] The method for obtaining black and white box experiment indicators includes: Place the mouse in the small door between the light and dark boxes, and start counting from the moment the mouse enters the black and white box; The movement distance and time of the mouse entering the white box within the set time were recorded.

[0037] Example 2: (1) Adaptive feeding: 24 eight-week-old adolescent mice were selected and randomly divided into two groups, with 12 mice in each group. The mice were then placed in an SPF-grade experimental animal room with 4 mice in a cage for adaptive feeding. The feeding environment temperature was set at 22°C and the humidity was set at 65% RH. The light was on from 9:00 to 21:00 and the dark was on from 21:00 to 9:00. The mice were fed for 5 days. During the adaptive feeding process, both groups of mice had free access to the same feed and water. (2) After the adaptive feeding in step (1), the two groups of mice were divided into a control group (Ctrl) and an experimental group (THz). The experimental group (THz) received fixed-point head terahertz irradiation for 15 minutes per day for 5 consecutive days (terahertz frequency: 0.22 THz, power density: 200 mW / cm 2 The control group Ctrl and the experimental group THz underwent the same operation, but the terahertz source was stopped. During the terahertz irradiation process, both groups of mice were free to eat the same feed and drink water; (3) Behavioral testing of mice: After irradiation in step (2), the control group Ctrl and experimental group THz mice were subjected to open field test, elevated plus maze test, three-chamber social test and black-white box test respectively.

[0038] Behavioral testing in the open field test: Mice were placed in the center of an open field. A monitoring instrument automatically measured the time the mouse spent in the center of the open field and the distance it traveled within the center of the open field over a 5-minute period. The monitoring data are shown in Table 1.

[0039] Assuming the preset threshold is 0.05, the monitoring results show that the mean time spent in the center of the open field by the THz mice in the experimental group was 20.5975±4.5628s, while the mean time spent in the center of the open field by the Ctrl mice in the control group was 25.0383±4.6279s. There was a significant difference in the time spent in the center of the open field between the THz mice in the experimental group and the Ctrl mice in the control group (P value < 0.05). The mean movement distance of the experimental group THz mice in the center of the open field was 2489.1350±588.4541 mm, and the mean movement distance of the control group Ctrl mice in the open field was 2637.2350±630.2576 mm. There was a significant difference in the time spent in the center of the open field between the experimental group THz and the control group Ctrl (P value = 0.56).

[0040] Behavioral testing in the elevated plus maze: Mice were placed in the center of the plus maze, head-on toward the open arms. A monitoring instrument automatically measured the number of times the mouse entered the open arms and the duration of each entry over a 5-minute period. The monitoring data are shown in Table 1.

[0041] The monitoring results showed that the number of times the mice in the experimental group THz entered the open arm was 1.25±1.34, and the number of times the mice in the control group Ctrl entered the open arm was 3±1.95. There was a significant difference in the number of times the mice in the experimental group THz entered the open arm compared with the control group Ctrl (P value < 0.05). The time for THz mice in the experimental group to enter the open arm was 19.7275±4.1928s, and the time for Ctrl mice in the control group to enter the open arm was 31.3100±5.3205s. There was a significant difference in the arm opening time between the THz mice in the experimental group and the Ctrl mice (P value < 0.001).

[0042] Behavioral testing in a three-chamber social interaction experiment: Place the unfamiliar mouse in the cage of the left chamber, and the experimental mouse in the middle chamber. Open the sliding doors of both chambers, and a monitoring instrument automatically measures the number and duration of sniffs made by the mouse in the left cage over a 5-minute period. See Table 1 for the monitoring data.

[0043] The monitoring results showed that the number of sniffings in the left cage by the mice in the experimental group THz was 5.67±2.35, and the number of sniffings in the left cage by the mice in the control group Ctrl was 8.83±2.86. There was a significant difference in the number of sniffings in the left cage between the experimental group THz and the control group Ctrl (P value < 0.01); The sniffing time of the THz mice in the experimental group in the left cage was 22.5825±3.9254s, and the sniffing time of the Ctrl mice in the control group in the left cage was 26.3467±4.9321s. There was a significant difference in the arm opening time between the THz mice in the experimental group and the Ctrl mice (P value = 0.05).

[0044] Behavioral testing in the black-white box experiment: Mice were placed through a small door between light and dark boxes. Time was measured from the moment the mouse entered the black-white box. A monitoring instrument automatically measured the distance and time the mouse spent in the white box over a 5-minute period. The monitoring data are shown in Table 1.

[0045] The monitoring results showed that the movement distance of the mice in the experimental group THz entering the white box was 7840.3650±700.0329mm, and the movement distance of the mice in the control group Ctrl entering the white box was 8289.9250±674.3345mm. There was a significant difference in the movement distance of the mice in the experimental group THz and the control group Ctrl entering the white box (P value = 0.0123); The time it took for the THz mice in the experimental group to enter the white box was 171.1942±11.3573s, and the time it took for the Ctrl mice in the control group to enter the white box was 179.3792±12.5465s. There was a significant difference in the arm opening time between the THz mice in the experimental group and the Ctrl mice in the control group (P value = 0.0108).

[0046]

[0047] Table 1

[0048] This shows that the present invention successfully established an anxiety mouse model based on terahertz waves.

[0049] In summary, the method for constructing a mouse model provided by the present invention is to model the model in a magnetic field environment, obtain experimentally verified results, and successfully establish a model of anxious mice based on terahertz waves.

[0050] The above embodiments are only used to illustrate the technical method of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical method of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical method of the present invention.

Claims

1. A method for constructing an anxiety mouse model based on terahertz waves, characterized in that: include: Several mice were randomly assigned into two groups; Two groups of mice were subjected to adaptive housing and inverted light cycle housing; One of the two groups of mice after feeding was used as the experimental group, and the other was used as the control group; A terahertz wave irradiation platform for mice was constructed to irradiate mice in the experimental and control groups; The irradiated mice in the control and experimental groups were subjected to behavioral tests including open field test, elevated plus maze test, three-chamber social test and black-and-white box test.

2. The method for constructing an anxiety mouse model based on terahertz waves according to claim 1, characterized in that: The adaptive breeding comprises: Both groups of mice were placed in an SPF-grade experimental animal room; The mice are raised for a certain period of time according to preset environmental conditions; wherein the preset environmental conditions include: the optimal survival temperature and optimal survival humidity of the mice.

3. The method for constructing an anxiety mouse model based on terahertz waves according to claim 1, characterized in that: The inverted photoperiod breeding includes: The preset environmental conditions of the two groups of mice were kept unchanged, and the two groups of mice after adaptive feeding were raised under a 12-hour inverted light cycle for a certain period of time.

4. The method for constructing an anxiety mouse model based on terahertz waves according to claim 1, characterized in that: The method of constructing a terahertz wave irradiation platform for mice to perform irradiation treatment on the mice in the experimental group and the control group includes: A1: The terahertz wave irradiation platform for mice in the experimental group irradiates the head with terahertz waves at a fixed point according to the preset irradiation time; A2: Stop the terahertz source and perform the same operation as step A1 on the control group mice.

5. The method for constructing an anxiety mouse model based on terahertz waves according to claim 4, characterized in that: The method for constructing a terahertz wave irradiation platform for mice comprises: The terahertz wave irradiation platform for mice is composed of a terahertz source, a plane mirror, a polyethylene lens, a mouse holder, a bracket and a lifting platform, specifically: Place the terahertz emission source on a lifting platform; The plane mirror is fixed on the bracket at an angle of 45°, with the mirror facing downward; The polyethylene lens is fixed below the plane mirror of the bracket and placed horizontally so that the center of the lens and the center of the plane mirror are on the same vertical line; Adjust the height of the lifting platform so that the waveguide port of the terahertz emission source is aligned with the center of the plane mirror; Place the mouse holder under the center of the lens.

6. The method for constructing an anxiety mouse model based on terahertz waves according to claim 1, characterized in that: The irradiated mice in the control group and experimental group were subjected to behavioral tests including open field test, elevated plus maze test, three-chamber social interaction test and black-white box test, respectively. The mice in the control group and the experimental group were subjected to the open field test, elevated plus maze test, three-chamber social test and black-white box test, respectively, and the corresponding indicator data of each experiment were recorded; among them, the indicator data included: open field test indicators, elevated plus maze test indicators, three-chamber social test indicators and black-white box test indicators; open field test indicators included: the time the mouse spent in the center of the open field and the distance moved in the center of the open field; elevated plus maze test indicators included: the number and time the mouse entered the open arm; three-chamber social test indicators included: the number and time the mouse sniffed in the left cage; black-white box test indicators included: the distance and time the mouse entered the white box; Calculate the mean and standard deviation of the indicator data corresponding to the experimental group and the control group in each experiment, and use the independent sample t test to calculate the P value of the corresponding indicator of the experimental group and the control group; If the P value is less than or equal to the preset threshold, it means that the difference in indicator data between the experimental group and the control group is statistically significant, that is, terahertz wave irradiation successfully induces anxiety-like behavior in mice, and the anxiety mouse model is successfully established.

7. The method for constructing an anxiety mouse model based on terahertz waves according to claim 6, characterized in that: The method for obtaining the open field test index comprises: The mouse was placed in the center of the open field, and the timing began when the mouse entered the open field; The time the mouse spends in the center of the open field and the distance it moves in the center of the open field are recorded within the set time.

8. The method for constructing an anxiety mouse model based on terahertz waves according to claim 6, characterized in that: The method for obtaining the elevated plus maze test index comprises: It was set that the mouse's hind legs entered the open arm 5 cm once; The mouse was placed in the center of the plus maze with its head facing the open arm, and the timing began when the mouse entered the plus maze; The number and time of mice entering the open arms within the set time were recorded.

9. The method for constructing an anxiety mouse model based on terahertz waves according to claim 6, characterized in that: The method for obtaining the three-chamber social experiment indicator includes: A sniff was counted as one time when the mouse's head was within 3 cm of the left cage and stayed there for at least 1 second; Place the stranger mouse in the cage of the left box, place the mouse in the middle box, open the sliding doors of the left and right boxes, and start timing from the moment the mouse enters the middle box; The number and duration of sniffing by the mouse in the left cage were recorded within the set time.

10. The method for constructing an anxiety mouse model based on terahertz waves according to claim 6, characterized in that: The method for obtaining the black-and-white box experiment index includes: Place the mouse in the small door between the light and dark boxes, and start counting from the moment the mouse enters the black and white box; The movement distance and time of the mouse entering the white box within the set time were recorded.

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