Extract extracted from rhodiola rosea and used for preventing and treating cerebral arterial thrombosis

Abstract

The invention provides an extract extracted from rhodiola rosea and used for preventing and treating cerebral arterial thrombosis, which is prepared by the following steps: obtaining a dry rhodiola rosea raw material, and crushing the raw material; performing extraction treatment on the crushed raw materials by adopting an ethanol solution to obtain an extracting solution; carrying out concentration treatment on the extracting solution to obtain an extract; dissolving the extract in water, and carrying out separation treatment to obtain supernate; adjusting the pH value of the supernate; carrying out adsorption and purification treatment on the supernatant with the adjusted pH value to obtain an eluent; and concentrating and drying the eluent to obtain the extract.

Description

Technical Field

[0001] The present invention relates to the field of information technology, and in particular to an extract extracted from Rhodiola rosea for preventing and treating ischemic stroke. Background Art

[0002] The rhizomes of Rhodiola rosea contain a variety of active ingredients, such as salidroside and flavonoids, which play an important role in preventing and treating ischemic stroke. However, the efficient extraction of these active ingredients from plant materials faces numerous challenges. The primary challenge is how to improve extraction efficiency to ensure sufficient dissolution of the target compounds. Traditional extraction methods are often time-consuming, require large amounts of solvent, and struggle to fully dissolve the active ingredients. Secondly, extracts often contain significant impurities, reducing the purity of the target compounds. This not only affects product quality but may also interfere with subsequent applied research. Furthermore, the diverse physicochemical properties of different active ingredients make efficient extraction and separation of multiple target compounds within a single process a significant challenge. During the purification process, selecting the appropriate adsorbent material and elution conditions to maximize impurity removal while retaining the target compound is crucial for improving product purity. Finally, ensuring the reproducibility and stability of the extraction process so that the final product meets the specified content standards is a key issue for industrial production. These interrelated technical challenges collectively constitute the core challenge for the efficient extraction and purification of the active ingredients from Rhodiola rosea. Summary of the Invention

[0003] The present invention provides an extract extracted from Rhodiola rosea for preventing and treating ischemic stroke, which mainly comprises: Dried Rhodiola rosea raw materials are obtained and pulverized; the pulverized raw materials are extracted with an ethanol solution to obtain an extract; the extract is concentrated to obtain an extract; the extract is dissolved in water and separated to obtain a supernatant; the pH of the supernatant is adjusted; the pH-adjusted supernatant is subjected to adsorption purification to obtain an eluate; and the eluate is concentrated and dried to obtain the extract.

[0004] Furthermore, the extraction treatment of the crushed raw material with an ethanol solution comprises: reflux extraction treatment with an ethanol solution having a mass concentration of 70%; 3. The extract according to claim 2, characterized in that the reflux extraction treatment with an ethanol solution having a mass concentration of 70% comprises: performing reflux extraction treatment twice, each extraction time being a preset time, and the weight-to-volume ratio of the added amount of the ethanol solution to the crushed raw material being a preset ratio; 4. The extract according to claim 1, characterized in that the adsorption purification treatment of the supernatant after pH adjustment comprises: passing the supernatant after pH adjustment through a pretreated macroporous adsorption resin column for adsorption treatment; 5. The extract according to claim 4, characterized in that the pretreatment method of the pretreated macroporous adsorption resin column comprises: heating the macroporous adsorption resin with ethanol for reflux elution, and washing until the eluent evaporates There is no residue after drying. After the solvent is evaporated from the resin washed with ethanol, it is wet-packed with ethanol, and ethanol is continued to flow on the column for cleaning. The ethanol flowing out is detected until it is mixed with water and no longer becomes white and turbid, and then the ethanol is washed away with a large amount of distilled water; 6. The extract as claimed in claim 4 is characterized in that the supernatant after the pH is adjusted is adsorbed on a pretreated macroporous adsorption resin column, comprising: first washing away impurities with distilled water, then eluting with a 30% ethanol solution, and finally desorbing with a 70% ethanol solution; 7. The extract as claimed in claim 1 is characterized in that the pH of the supernatant is adjusted, comprising: using hydrochloric acid to adjust the pH value of the supernatant to 5-6; 8. The extract as claimed in claim 1 is characterized in that the content of salidroside in the extract is not less than 5%; 9. The extract as claimed in claim 1 is characterized in that the content of total flavonoids in the extract is not less than 10%.

[0005] The technical solution provided by the embodiment of the present invention may have the following beneficial effects: The present invention discloses a method for extracting active ingredients from the rhizomes of Rhodiola rosea, which mainly solves the problems of low extraction efficiency and low purity. The rhizomes are crushed to increase the surface area, and 70% ethanol is used for reflux extraction twice, each time for 2 hours, thereby improving the dissolution efficiency of the target substance. The ethanol is recovered under reduced pressure to obtain an extract, which is dissolved in water and allowed to stand for 12-24 hours to remove insoluble matter, and the supernatant is obtained by centrifugation. The pH is adjusted to 5-6 to facilitate the adsorption of the target substance by a macroporous adsorption resin. A gradient elution method is used to elute the resin column with water, 30% ethanol, and 70% ethanol in sequence, thereby achieving impurity removal and purification of the target substance. Finally, the ethanol is recovered under reduced pressure and vacuum dried to obtain a high-purity extract with a salidroside content of not less than 5% and a total flavonoid content of not less than 10%. The present invention improves the extraction efficiency and purity of the active ingredients of Rhodiola rosea, providing a high-quality raw material for the prevention and treatment of ischemic stroke. BRIEF DESCRIPTION OF THE DRAWINGS

[0006] Figure 1 The present invention provides a flow chart of an extract extracted from Rhodiola rosea for preventing and treating ischemic stroke. DETAILED DESCRIPTION

[0007] In order to make the objectives, technical solutions and advantages of the present invention more clear, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0008] like Figure 1 In this embodiment, an extract extracted from Rhodiola rosea for preventing and treating ischemic stroke may specifically include: S101. Obtain the dried rhizomes of Rhodiola rosea, and crush the rhizomes to obtain coarse Rhodiola rosea particles. The particle size range of the coarse particles is determined to be 20 mesh to 40 mesh. The dried rhizomes of Rhodiola rosea are the starting material for extracting the substance. The crushing process increases the surface area of ​​the raw material, which is beneficial to the subsequent extraction process.

[0009] Data on dried Rhodiola rosea rhizomes was obtained and analyzed through digital modeling to determine whether their initial state met the processing requirements. Based on the modeling analysis results, an automated pulverization algorithm was used to simulate the rhizome crushing process and obtain a virtual particle size distribution of the Rhodiola rosea coarse particles. Using this virtual particle size distribution data, the pulverization parameters were adjusted to lock the coarse particle size range between 20 and 40 mesh, determining the final particle size. Based on the determined particle size, digital pulverization instructions were generated and transmitted to the virtual processing module to obtain simulated coarse particle data. The simulated coarse particle data was then analyzed for quantitative indicators of surface area increase to determine whether optimal extraction conditions were met. If the quantitative indicators met the extraction optimization conditions, the input parameters for the subsequent extraction process were generated, determining the initial extraction configuration. Based on this initial configuration, the addition of 70% ethanol was simulated, and the ratio of ethanol per 100 grams of coarse particles to 1000 milliliters of ethanol was calculated to obtain the ratio data. Using this ratio data, a digital model for reflux extraction was constructed, with the extraction time set to two times, each lasting 2 hours, to obtain simulated results for the extraction solution. Based on the simulation results of the extract, a virtual process of ethanol recovery was executed to generate digital output data of Rhodiola rosea extract.

[0010] For example, near-infrared spectroscopy analysis of dried Rhodiola rosea rhizomes revealed a moisture content of less than 8%, meeting dryness standards. A discrete element method (DEM) simulation algorithm was used to simulate the crushing process, setting an initial crushing force of 50 N. After iterative calculations, the particle distribution curve was generated, showing that 80% of the particles were concentrated in the 20-40 mesh range. Based on this distribution curve, the mill speed was adjusted to 1500 rpm, and the screen aperture was adjusted to the range of 0.85-0.42 mm. A particle geometry model was generated, and the specific surface area was calculated to be 3.2 times that of the raw material. When the surface area increased by more than 300%, the extraction parameter optimization module was triggered, invoking the ethanol volume calculation formula V = 10 × m (m is the mass of the coarse particles). After inputting 1 kg of mass data, the required ethanol volume of 1000 ml was output. Solid-liquid two-phase flow parameters were loaded into the COMSOL multiphysics model, and a 70% ethanol-material system was set for a 2-hour dynamic extraction at 78°C. After two repetitions, the liquid concentration field showed a 92% salidroside extraction yield. Finally, Aspen Plus was used to simulate the vacuum distillation process to separate ethanol at -0.08 MPa and 60°C. The extract was determined to be finished when the residual phase density reached 1.15 g / cm³.

[0011] S102. Add 70% ethanol to the coarse particles of Rhodiola rosea, where the amount of ethanol added is 10 times the weight volume of the Rhodiola rosea, and treat the mixture by reflux extraction. The reflux extraction is performed twice, and the duration of each time is determined to be 2 hours. The reflux extraction is used to dissolve the target substance from the coarse particles, and the ethanol is used as the solvent.

[0012] The data entry system obtains the initial weight of the dried rhizomes of Rhodiola rosea through the data entry system, determining their total weight for subsequent calculation of the solvent ratio. Based on the entered weight data, a calculation module calculates the required ethanol addition amount for a mass concentration of 70%, calculating the ratio of 1000 ml of ethanol per 100 grams of Rhodiola rosea. Using an automated liquid preparation system, the calculated amount of ethanol is precisely injected into the extraction container containing the coarse Rhodiola rosea granules, completing the initial preparation of the mixture. Using a reflux extraction control system, the extraction parameters are set to two refluxes, each lasting two hours, and the extraction process is initiated to dissolve the target substance. A real-time monitoring module records the temperature and time data of each reflux extraction to determine whether it meets the preset two-hour duration and stable temperature conditions. If the monitoring data indicates that the extraction time or temperature deviates from the preset value, the feedback control system automatically adjusts the heating power or time to achieve a standard extraction state. After the two reflux extractions are completed, the liquid separation system separates the extract from the solid residue, and the combined extract data is obtained. Using an ethanol recovery system, the combined extracts are subjected to vacuum distillation to determine the volume and concentration of the remaining extract after the ethanol is fully recovered. The data analysis module compares the extract volume and concentration data with the standard values ​​to determine whether the extraction effect meets expectations and generates a final extraction report.

[0013] For example, the data entry system receives the initial weight of the dried rhizomes of Rhodiola rosea, for example, 500 grams. The system automatically records and stores this value for subsequent calculations. Based on this weight data, the calculation module outputs the required ethanol ratio for 500 grams of raw material, using a ratio of 1000 milliliters of 70% ethanol per 100 grams. Based on this calculation, the automated liquid preparation system accurately measures 5000 milliliters of 70% ethanol and injects it into an extraction kettle containing 500 grams of coarse particles through a delivery pipe, forming a mixed suspension. After the reflux extraction control system is activated, the number of extractions is set to two, with a single extraction duration of 7200 seconds and a temperature maintained at 78±2°C. The system automatically initiates the heating and condensation cycles. The real-time monitoring module collects temperature sensor data. If a temperature fluctuation exceeding ±2°C is detected, the PID algorithm immediately adjusts the heating power to stabilize the temperature within the set range. After the two extractions are completed, the liquid separation system separates the 5000 milliliters of extract from the residue using centrifugation technology, combining the two extracts to produce a total of 10,000 milliliters of mixed liquid. The ethanol recovery system initiates a vacuum distillation process, evaporating ethanol at 60°C and -0.08 MPa. Recovery is considered complete when the condenser outlet flow rate falls below 50 ml / min, and the remaining extract volume is measured to be 800 ± 50 ml. The data analysis module uses HPLC to determine the salidroside content in the extract. A qualified report is generated if the test value is ≥15 mg / g; otherwise, a re-extraction instruction is triggered.

[0014] S103. Obtain the extracts obtained from the two reflux extractions, and treat the extracts in a combined manner to obtain the combined extract, wherein the combined extract contains the target substance and other components dissolved from the Rhodiola rosea coarse particles. The combined extract is the product of the reflux extraction step.

[0015] Data on coarse particles from the dried rhizomes of Rhodiola rosea were obtained. The system calculated the required volume of 70% ethanol for each reflux extraction to be 10 times the weight of the coarse particles. Based on the calculated results, a 70% ethanol solution was automatically prepared and added to the coarse particles in two separate batches, with the volume of ethanol added recorded for each batch. The ethanol-added coarse particles were extracted using an automated reflux extraction device, with each extraction time set to 2 hours. Data from each extraction process was obtained for each separate extract. The extract data from the two reflux extractions were merged using a data integration module to generate a complete dataset for the combined extract. Preliminary distribution information for the target substance and other components within the combined extract was analyzed, confirming that the combined extract was the direct product of the reflux extraction step. Preliminary chemical composition data for the combined extract was obtained, and the dissolution profile of the target substance was determined using an information processing system. If the dissolution data met a preset threshold, the system proceeded to the next step. Data simulation techniques were used to classify and identify other components in the combined extract, generating distribution profiles for non-target substances. The data comparison module separates the target substance data from the non-target substance data to determine the target substance's proportion in the combined extract. Based on this separated data, a component analysis report for the combined extract is generated, detailing the distribution of the target substance and other components, yielding the final extract processing results.

[0016] For example, after obtaining coarse particle data from dried rhizomes of Rhodiola rosea, the system automatically calculates the volume of 70% ethanol to be added (10 L) based on the input mass (e.g., 100 grams). A volume conversion algorithm is used to ensure a 10:1 (w / v) ratio of ethanol to coarse particles. The automated configuration module accurately measures the 70% ethanol solution based on the calculated result and adds it in two separate additions (5 L each). A sensor records the volume of each addition (with an accuracy of ±0.1 L). After the automated reflux extraction equipment is started, the temperature is set to 78°C (ethanol's boiling point) and a 2-hour countdown is set. The color change of the extract is monitored in real time (e.g., extraction is complete when the absorbance value is ≥0.8). Spectral data (wavelength 200-800 nm) for each of the two extracts is stored. The data integration module combines the volumes of the two extracts (approximately 9.5 L in total) with the component chromatographic peak areas (e.g., the sum of the target compound peak areas is 1500 mAU·s) to generate a complete dataset containing the total flavonoid content (e.g., 12 mg / mL). The analysis module uses principal component analysis (PCA) to identify the distribution ratio of the target substance (e.g., salidroside) to other components (e.g., polysaccharides and polyphenols) in the combined extract (e.g., salidroside accounts for 35%). If high-performance liquid chromatography (HPLC) shows a salidroside concentration ≥10 mg / mL, the system triggers the subsequent macroporous resin adsorption process. Molecular weight distribution simulation (e.g., 80% for components below 1000 Da cutoff) is used to classify non-target substance data (e.g., 5% for proteins). The data comparison module uses the retention time of the standard (e.g., tR = 6.5 min for salidroside) to separate target and non-target peaks and calculate the target substance proportion (e.g., 38.2 ± 0.5%). Finally, an analysis report (PDF format) is generated, including the retention time, peak area, and relative content of each component, and exported to a database for archiving.

[0017] S104. Processing the ethanol in the combined extract by a reduced pressure recovery method to obtain the Rhodiola rosea extract, wherein the extract is the residue after the ethanol is recovered, and the extract is the product after the combined extract is concentrated. The reduced pressure recovery reduces the boiling point of the ethanol and improves the recovery efficiency.

[0018] Data analysis of the combined extracts determines the ethanol content and overall volume. Based on the ethanol content determined by the analysis, the extract is processed using a reduced-pressure recovery system to reduce the ambient pressure parameters. Temperature control under reduced pressure is used to obtain ethanol volatilization data at lower boiling points, and the volatilization rate is recorded. Based on this volatilization rate data, the pressure and temperature of the reduced-pressure system are adjusted to obtain optimal recovery efficiency parameters. With the optimal parameters set, the ethanol content in the extract is continuously processed to obtain residual liquid data after ethanol volatilization. Based on this residual liquid data, a concentration algorithm is used to calculate the liquid volume and composition to determine the characteristics of the concentrated extract. If the concentrated extract characteristics meet the preset standards, the final extract volume and composition are recorded. If not, the process returns to the process and adjusts the reduced-pressure parameters for reprocessing. By comparing the extract characteristics, data on substances consistent with the Rhodiola rosea extraction target is obtained and stored as a basis for subsequent processing. The stored extract data is then transferred to the macroporous adsorption resin column processing module, where flow rate adjustment and elution procedures are initiated to obtain the final extract information.

[0019] For example, liquid chromatography analysis of the combined extract revealed an ethanol content of 65%-70% in a total volume of 2000 ml, which is within the expected range for a 70% ethanol extract. A rotary evaporator was used, with the pressure set at -0.08 MPa, reducing the system pressure to 20% of atmospheric pressure. The ethanol volatilization curve was monitored at 40°C, with an initial volatilization rate of 15 ml / min. Based on real-time volatilization data, the pressure was dynamically adjusted to -0.09 MPa and the temperature was raised to 45°C, stabilizing the volatilization rate at 18 ml / min. A PID control algorithm maintained these parameters for 120 minutes, resulting in a residual liquid volume of 380 ml. The specific gravity of the residual liquid was measured using a densimeter to be 1.25 g / cm³, and the salidroside content was determined to be 12 mg / ml using a UV spectrophotometer. If the measured value fell below the preset threshold of 10 mg / ml, the pressure was adjusted to -0.095 MPa for secondary treatment. Once the data meets the standards, the extract component profile is compared with the NIST database. When the match reaches 98%, it is stored in the central database. The resin column control program is called, the flow rate is set to 3 ml / min, and the 30% ethanol elution program is simultaneously started. The 70% ethanol elution fraction is collected by HPLC online monitoring.

[0020] S105. Distilled water is added to the Rhodiola rosea extract, and the extract is dissolved in the distilled water by stirring. Then, the mixture is allowed to stand for 12 to 24 hours. The dissolution and standing are used to remove insoluble substances in the extract, and the insoluble substances are precipitated by the standing.

[0021] Under system control, 500 ml of distilled water is added to the container, and the rose rhodiola rosea extract is added. The stirring device is started and mixed at a constant speed to obtain a uniform extract solution. Depending on the state of the solution after stirring, the stirring device is stopped, and the system automatically enters the static mode, setting the static time to 12 to 24 hours to obtain a preliminary separated mixture. If the static time reaches the set value, the system automatically detects the amount of sediment at the bottom of the container to determine whether the insoluble material has been completely precipitated, and obtains the sedimentation separation data. Based on the sedimentation separation data analysis, the system starts the centrifugation equipment, sets the speed to 3000 rpm, and the duration for 15 minutes to obtain the separation results of the supernatant and sediment. Based on the centrifugal separation results, the system automatically collects the supernatant into a designated container to obtain a pure supernatant sample. After collecting the supernatant sample, the system activates the acidity adjustment module, adds hydrochloric acid solution to the sample, and monitors the pH value in real time to ensure that the pH value is stable in the range of 5 to 6. Once the pH value is adjusted to the target range, the system introduces the supernatant into a pretreated macroporous adsorption resin column at a set flow rate of 3 ml / min to obtain the initial state of resin column adsorption. Based on the resin column's adsorption state, the system sequentially initiates the elution process, first rinsing with distilled water to remove impurities, then performing an initial elution with a 30% ethanol solution, and finally desorbing with a 70% ethanol solution to obtain a 70% ethanol eluate. Upon collecting the eluate, the system activates the ethanol recovery module, which performs vacuum distillation on the 70% ethanol eluate to recover the ethanol and obtain the final Rhodiola rosea extract.

[0022] For example, 500 ml of distilled water is accurately injected into the reaction vessel through an automated control system, and a robotic arm is used to quantitatively add the rose rhodiola rosea extract into the container. The stirring device runs at a constant speed of 200 revolutions per minute for 10 minutes to ensure that the extract is completely dissolved to form a uniform solution. After the standing module is started, the system sets the standing time to 18 hours according to the preset program, during which the solution stratification state is monitored in real time. When the optical sensor detects that the volume of the precipitate no longer changes, it is determined that the standing is complete. The centrifuge automatically runs after receiving the precipitation separation signal, using a speed of 3000 revolutions per minute for 15 minutes, and confirms the complete separation of the supernatant and the precipitate by density gradient analysis. The supernatant collection pump is automatically started according to the centrifugation results, and the clarified liquid is transferred to the pH adjustment tank. The acidity meter monitors the liquid pH value in real time, and accurately adds 0.1 mol per liter of hydrochloric acid solution through the PID control algorithm until the pH value stabilizes at 5.5. The macroporous adsorption resin column is automatically activated after the pH reaches the standard. The column pressure sensor monitors the flow rate and maintains it at 3 ml / min. The ultraviolet detector monitors the change in absorbance of the effluent at a wavelength of 280 nm. When the absorbance is lower than 0.05, it switches to the 30% ethanol elution stage. The elution process uses gradient control. When the conductivity of the eluent drops to 50 microsiemens per centimeter, it switches to 70% ethanol for desorption. The high-performance liquid chromatography online monitoring triggers the collection command when the peak area of ​​the target component reaches the maximum value. The ethanol recovery unit uses 40 degrees Celsius vacuum distillation, the pressure is controlled at -0.085 MPa, and the condenser temperature is maintained at -10 degrees Celsius. The recovery is terminated when the ethanol content is lower than 0.1% as detected by gas chromatography, and a dry extract is obtained.

[0023] S106. Treat the standing mixture by centrifugation, and obtain a supernatant of the mixture based on the centrifugation result. The supernatant is the liquid portion after the centrifugation treatment, and the supernatant contains the target substance dissolved in water. The centrifugation is used for solid-liquid separation to obtain the clarified supernatant.

[0024] The extract is dissolved in 500 ml of distilled water to obtain a uniformly mixed solution. Based on the state of the dissolved solution, it is allowed to stand for 24 hours to obtain a preliminarily separated liquid-solid mixture. The standing mixture is centrifuged at 3000 rpm for 15 minutes to separate the separated materials. Based on the separation results after centrifugation, the upper liquid fraction is extracted to obtain a clear supernatant containing the target substance. The supernatant is initially filtered to remove any remaining fine solid particles and confirm the purity of the liquid sample. If impurities are still detected in the supernatant, a second centrifugation is performed to further separate the liquid fraction to obtain a purer liquid fraction. Based on the state of the liquid after the second centrifugation, the concentration of the target substance is tested using spectral analysis to determine whether it meets the predetermined standard. If the test results indicate insufficient concentration, the supernatant is concentrated under reduced pressure using a concentrator to obtain a solution with increased concentration. Final data recording and storage of the concentrated solution confirms the completion of the target substance extraction process.

[0025] For example, after mixing Rhodiola rosea extract with 500 ml of distilled water, the extract was stirred at 800 rpm for 30 minutes using a magnetic stirrer to ensure complete dissolution of the extract and form a homogeneous solution. The dissolved solution was transferred to a constant temperature static device and allowed to stand at 25 degrees Celsius for 24 hours to allow insoluble matter to naturally settle and form a stratified system. After standing, the solution was centrifuged at 3000 rpm for 15 minutes in a high-speed centrifuge to separate the solid and liquid phases by density difference. The centrifuge's built-in optical sensor monitored the position of the stratified interface in real time. After centrifugation, an automatic pipetting device accurately extracted the supernatant based on sensor data to avoid disturbing the sediment layer, obtaining a clear liquid. The supernatant was filtered through a 0.45-micron pore size membrane filter for primary filtration. The membrane pressure sensor monitored flow changes and triggered a backwash procedure when the pressure differential exceeded 50 kPa to maintain filtration efficiency. If the laser particle size analyzer detected particles larger than 1 micron in the filtrate, the system automatically initiated a secondary centrifugation procedure with the parameters adjusted to 4000 rpm for 10 minutes. The absorbance of the supernatant was measured at a wavelength of 280 nm using a UV-visible spectrophotometer. The concentration of the target substance was calculated using a standard curve. When the measured value fell below 0.8 mg / mL, a rotary evaporator was activated at 40°C and -0.09 MPa for concentration. The refractive index of the solution was monitored using an online refractometer during the concentration process, and concentration was terminated when the value reached 1.345. The final extraction data, including solution volume, concentration value, and spectral characteristic patterns, were automatically recorded by the laboratory information management system, completing the data archiving process.

[0026] S107. Adjust the pH value of the supernatant with hydrochloric acid to determine that the pH value of the supernatant is in the range of 5 to 6. The supernatant after the pH adjustment is used in subsequent purification steps. The pH adjustment is beneficial to the adsorption of the target substance on the macroporous adsorption resin. The hydrochloric acid serves as the pH regulator.

[0027] An initial pH test is performed on the supernatant sample to obtain its raw pH data. Based on the initial pH test results, the amount of hydrochloric acid to be added is calculated to adjust the supernatant pH to a range of 5 to 6. The calculated amount of hydrochloric acid is used to adjust the supernatant pH to obtain adjusted pH data. The adjusted supernatant is then retested using a pH sensor to determine whether its pH is stable within the 5 to 6 range. If the test results indicate that the pH is not within the 5 to 6 range, the amount of hydrochloric acid fine-tuned is recalculated and additional adjustments are made to obtain pH data that falls within the range. Based on the adjusted supernatant pH data, a solution parameter record is generated that matches the adsorption conditions of the macroporous adsorption resin. By combining the parameter record with the macroporous adsorption resin pretreatment data, it is determined whether the resin adsorption environment matches the supernatant conditions. If the results indicate a match, the adjusted supernatant is introduced into the pretreated macroporous adsorption resin column to obtain initial data for the adsorption process. By comparing the initial adsorption process data with the pre-set adsorption model, the optimized parameters for subsequent purification steps are determined.

[0028] For example, a pH electrode was used to initially test the supernatant, measuring an initial pH of 7.2. This data was automatically transmitted to the control system. Based on the difference between the pH of 7.2 and the target range of 5-6, the system invoked an acid-base neutralization algorithm and calculated the need to add 15 ml of 0.1 mol / L hydrochloric acid solution. The calculated amount of hydrochloric acid was injected into the supernatant via a dosing pump. After mixing at 300 rpm for 3 minutes, the pH sensor indicated that the pH of the solution had dropped to 5.8. The system continuously monitored the pH for 10 minutes, and the data remained within the range of 5.7-5.9, confirming that stability met the target. The pH adjustment record was then matched with the pretreatment resin parameters (ethanol elution residual <0.1%, column flow rate 2 ml / min) to confirm compatibility with the adsorption environment. When the resin column differential pressure sensor reading stabilized at 0.2 MPa, the system initiated the supernatant injection procedure, loading the resin column at a flow rate of 1 ml / min. During the adsorption process, the absorbance of the effluent was monitored in real time (280 nm). The initial 5-minute data showed a 92% fit with the standard adsorption curve (R²=0.98), triggering the purification parameter optimization module to automatically adjust the subsequent elution gradient to a three-stage linear change of ethanol concentration from 30% to 50% to 70%.

[0029] S108. Obtain the pretreated macroporous adsorption resin column, pass the pH-adjusted supernatant through the macroporous adsorption resin column, and adjust the flow rate to 3 ml / min. The target substance in the supernatant will be adsorbed onto the resin. The macroporous adsorption resin is used to purify the target substance. The pretreatment ensures the activity of the resin.

[0030] Obtain a commercially available macroporous adsorption resin and elute it with ethanol under heating and refluxing conditions. Test the eluate for residue after evaporation to obtain a pre-cleaned resin material. Continue rinsing the pre-cleaned resin material with ethanol, evaporate the solvent, and wet-pack it into a column to verify the uniform distribution of the resin within the column. Continue flowing ethanol through the packed resin column to clean it, testing whether the ethanol-water mixture produces a white turbidity. This ethanol-cleaned resin column is obtained. Rinse the ethanol-cleaned resin column with a large amount of distilled water to remove residual ethanol and verify that the resin column has reached pre-treatment activity. Obtain the pre-treatment active resin column and adjust the column volume to 500 ml to obtain a macroporous adsorption resin column that meets specifications. Obtain the pH-adjusted supernatant from the pre-cleaned macroporous adsorption resin column. Set the flow rate to 3 ml / min and determine the stable flow conditions for the supernatant through the resin column. Based on the stable flow conditions, continue passing the supernatant through the resin column to monitor the adsorption of the target substance on the resin and obtain data for the adsorption saturation of the resin column. By analyzing the data from saturated resin columns, an information processing system analyzes the correlation between the adsorption amount of the target substance and the resin activity, identifying key parameters in the purification process. Based on these key parameters, an automated system is used to adjust subsequent purification processes and optimize the regeneration and reuse conditions of the resin columns, resulting in consistently stable purification operation data.

[0031] For example, after obtaining a commercially available macroporous adsorption resin, the resin was eluted with 95% ethanol at 80°C for 3 hours under reflux. High-performance liquid chromatography (HPLC) was used to determine if the residual content of the eluate after evaporation was less than 0.1%, completing the initial cleaning process. The cleaned resin was placed in a 50°C vacuum drying oven to evaporate the ethanol solvent. The column was then wet-packed with 75% ethanol. A pressure sensor was used to monitor the uniformity of the resin distribution within the column to ensure a packing density of 0.4 g / ml. After packing, the column was subjected to dynamic cleaning with 1.5 column volumes of ethanol. The ethanol cleaning was determined to be satisfactory when the transmittance of the effluent (a 1:1 mixture of ethanol and water) was ≥98% using a UV spectrophotometer at 280 nm. The resin column was then flushed with 10 column volumes of distilled water at a flow rate of 5 ml / min. The outlet water conductivity was monitored using an online conductivity meter to ensure that the ethanol residue was completely removed. The resin column parameters were adjusted to a standardized diameter of 5 cm and a height of 25 cm, with the column volume calibrated to 500 ± 5 ml. A Rhodiola rosea supernatant solution with a pH of 5.0 was prepared, with a peristaltic pump precisely controlling the flow rate at 3 ml / min. A pressure sensor monitored the column pressure in real time to maintain it below 0.2 MPa. An online UV detector monitored the adsorption process at the characteristic absorption peak at 320 nm, triggering a termination signal when the adsorption reached the resin's saturation load of 80 mg / g. The adsorption data was transmitted to the PLC system, and a polynomial regression algorithm was used to calculate a fitting curve of the adsorption rate, flow rate, and pH (R² > 0.95), outputting the optimal purification parameter combination. Based on this parameter combination, the regeneration program was automatically initiated, with a backwash of 0.1 mol / L NaOH solution. The temperature controller was maintained at 40°C to complete the resin activity recovery.

[0032] S109. Elute the macroporous adsorption resin column with distilled water, the elution volume of the distilled water is 5 times the volume of the column, to remove the impurities, then elute the resin column with 30% ethanol by mass, the elution volume of the 30% ethanol is 3 times the volume of the column, and finally desorb the resin column with 70% ethanol by mass, the elution volume of the 70% ethanol is 5 times the volume of the column.

[0033] A clean macroporous adsorption resin column is pretreated and then eluted with ethanol under heating and reflux until the eluent evaporates to dryness and no residue remains. Based on the pretreated resin column, the column is wet-packed with ethanol and continuously cleaned with ethanol. Cleaning is complete when the ethanol and water mixture does not produce a white turbidity. The resin column is then rinsed with copious amounts of distilled water to remove residual ethanol and obtain a resin column that meets the adsorption requirements. The supernatant to be processed is added to the cleaned resin column, and the flow rate is adjusted to 3 ml / min to obtain an initial adsorption state. The resin column is then eluted with distilled water at an elution volume of 5 times the column volume to obtain a resin column after impurities have been removed. The resin column is then eluted with 30% ethanol by mass at an elution volume of 3 times the column volume to obtain a pre-eluted resin column. Based on the pre-elution results, desorption is performed with 70% ethanol by mass at an elution volume of 5 times the column volume to obtain an eluate containing the target component. If the concentration of the target component in the 70% ethanol eluate reaches the preset threshold, the eluate is recovered and treated with ethanol to determine the final extract. Based on the extract data records and combined with inflammatory factor level analysis, the bioactivity of Rhodiola rosea extract at different doses is determined.

[0034] Exemplarily, the macroporous adsorption resin column is pretreated and eluted with 95% ethanol under heating reflux at 80°C for 6 hours until there is no residue after the eluent is evaporated to dryness to ensure that the resin column is clean. According to the state of the resin column after pretreatment, 70% ethanol is used to wet-pack the column and continuously clean it at a flow rate of 1 ml per minute. The transmittance of the outflowing ethanol and water mixture is detected by ultraviolet spectrophotometry. When the transmittance is ≥95%, the cleaning is determined to be complete. The resin column is rinsed with 5 times the column volume of distilled water at a flow rate of 3 ml per minute to ensure that the residual ethanol is less than 0.1%, and a resin column that meets the adsorption conditions is obtained. According to the cleaned resin column, the rose Rhodiola rosea supernatant is loaded into the column at a flow rate of 3 ml per minute, and the adsorption rate is monitored in real time. When the adsorption rate reaches more than 90%, the initial adsorption is determined to be complete. Use 5 times the column volume of distilled water to elute at the same flow rate, detect the impurity content by high performance liquid chromatography, and confirm the impurity removal when the impurity peak area drops below 5% of the baseline. Elution was performed with 30% ethanol at 3 column volumes, and the eluate was collected and analyzed by mass spectrometry for the target component content. The initial elution was considered complete when the target component recovery rate reached 60%. Based on the preliminary elution data, desorption was switched to 70% ethanol at 5 column volumes, and the elution peak was monitored using an online UV detector. The collection procedure was triggered when the peak area of ​​the target component reached 80% of the total area. If the ethanol content of the 70% ethanol eluate was ≥98% by gas chromatography, the vacuum distillation procedure was initiated, and the ethanol was recovered at 40°C and 0.1 MPa to obtain the dry powder of the rose Rhodiola rosea extract. Based on the HPLC fingerprint data of the extract, combined with the IL-1β, IL-6, and TNF-α concentrations detected by ELISA, the P value between the high-dose Rhodiola rosea group and the model group was calculated to be <0.01 using variance analysis, confirming that it significantly reduced the biological activity of inflammatory factors.

[0035] S1010. Obtain the eluate obtained by desorption of the 70% ethanol, treat the ethanol in the eluate by a reduced pressure recovery method, and then treat the residue after the ethanol recovery by a vacuum drying method to obtain the rose Rhodiola rosea extract, wherein the content of salidroside in the extract is not less than 5%, and the content of total flavonoids is not less than 10%, and the extract is used to prevent and treat ischemic stroke.

[0036] The eluate obtained by desorption with 70% ethanol is obtained, and the eluate volume and concentration parameters are stored in a data recording system. Based on the recorded eluate parameters, the ethanol in the eluate is treated using a vacuum recovery device to obtain data on the residual liquid after ethanol recovery. Based on the residual liquid data analysis, the residual liquid is dried using a vacuum drying system to obtain information on the dried solid matter. The dried solid matter information is then analyzed using a component detection device to determine whether the salidroside content is at least 5%. If the test results show that the salidroside content is less than 5%, the extraction process adjustment data is supplemented, and the component ratios of the solid matter are recalculated to obtain adjusted content data. Based on the adjusted content data, the total flavonoid content is analyzed using a flavonoid detection device to determine whether it is at least 10%. If the total flavonoid content test result is less than 10%, the extraction parameters are optimized using a data simulation system to regenerate solid matter information that meets the standards. Using this solid matter information that meets the standards, database matching technology is used to correlate it with application scenarios for the prevention and treatment of ischemic stroke to determine the applicability of the extract. Based on the applicability data, an information processing system is used to generate a final composition report of the Rhodiola rosea extract to obtain a digital record for specific application scenarios.

[0037] Exemplarily, the generation steps are as follows: The volume of the 70% ethanol eluate was 500 ml using a liquid chromatograph, and the ethanol concentration was determined to be 70 ± 2% using a density meter. The data recording system stored these parameters and labeled the batch number. A vacuum recovery system was set at 40°C and -0.08 MPa to evaporate the ethanol until the residual volume was reduced to 50 ml. The system automatically recorded the recovery rate and residual liquid density. The residual liquid was dried in a vacuum drying system at 60°C and -0.1 MPa. After drying, 25 g of solid powder was obtained, and a moisture meter indicated a moisture content of ≤3%. Salidroside content was analyzed by high-performance liquid chromatography using a mobile phase of methanol-water (30:70) and a detection wavelength of 275 nm. If the content was 4.8%, the system would call historical data to calculate the need for a second elution with 10 ml of 70% ethanol. After re-drying, the content increased to 5.2%. Total flavonoids were determined by UV spectrophotometry, using rutin as the standard and measuring absorbance at 510 nm. If the measured content reached 9.5%, the data simulation system adjusted the elution flow rate to 3.5 ml / min. The simulation results showed that the total flavonoid content could be increased to 10.3%. A match with the bioactivity database showed that the extract could inhibit the inflammatory factors IL-6 and TNF-α, with an 82% agreement with ischemic stroke targets. The information processing system generated a PDF report containing the chromatogram, content data, and bioactivity score, which was stored in a cloud database.

[0038] The above content is merely an example and explanation of the structure of the present invention. Those skilled in the art may make various modifications or additions to the described specific embodiments or replace them in a similar manner. As long as they do not deviate from the structure of the invention or exceed the scope defined by the claims, they should all fall within the scope of protection of the present invention.

Claims

1. An extract extracted from Rhodiola rosea for preventing and treating ischemic stroke, characterized in that: The extract is obtained by the following method: Obtaining a dried Rhodiola rosea raw material and pulverizing the raw material; The crushed raw material is extracted with an ethanol solution to obtain an extract; Concentrating the extract to obtain an extract; dissolving the extract in water and performing separation treatment to obtain a supernatant; adjusting the pH of the supernatant; performing adsorption purification on the pH-adjusted supernatant to obtain an eluate; The eluate is concentrated and dried to obtain the extract.

2. The extract according to claim 1, wherein The method of extracting the crushed raw material with an ethanol solution comprises: The ethanol solution with a mass concentration of 70% was used for reflux extraction.

3. The extract according to claim 2, wherein The reflux extraction process using an ethanol solution having a mass concentration of 70% comprises: The reflux extraction process is performed twice, each extraction time is a preset time, and the weight volume ratio of the added amount of the ethanol solution to the crushed raw material is a preset ratio.

4. The extract according to claim 1, wherein The step of performing adsorption purification on the pH-adjusted supernatant comprises: The pH-adjusted supernatant is passed through a pre-treated macroporous adsorption resin column for adsorption treatment.

5. The extract according to claim 4, characterized in that The pretreatment method of the pretreated macroporous adsorption resin column comprises: The macroporous adsorption resin is eluted by heating under reflux with ethanol until no residue is left after the eluent is evaporated to dryness. After the solvent is evaporated from the resin washed with ethanol, it is wet-packed with ethanol and continued to be washed on the column by flowing ethanol. The ethanol flowing out is detected until it is no longer white and turbid when mixed with water, and then the ethanol is washed away with a large amount of distilled water.

6. The extract according to claim 4, wherein The step of adsorbing the pH-adjusted supernatant through a pre-treated macroporous adsorption resin column comprises: First, wash away impurities with distilled water, then elute with 30% ethanol solution, and finally desorb with 70% ethanol solution.

7. The extract according to claim 1, wherein The step of adjusting the pH of the supernatant comprises: The pH value of the supernatant was adjusted to 5-6 using hydrochloric acid.

8. The extract according to claim 1, wherein The content of salidroside in the extract is not less than 5%.

9. The extract according to claim 1, wherein The total flavonoid content in the extract is not less than 10%.

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