Composition containing minocycline and application of composition in preparation of product for treating acne

Abstract

The invention relates to the technical field of medicines, and discloses a minocycline-containing composition and application of the minocycline-containing composition in preparation of a product for treating acne. 0.5%-2% of allantoin; 5%-9% of vitamin C; 2%-3% of vitamin E; 3%-7% of collagen; 1%-1.5% of a grape seed extract; 0.2% to 0.5% of ceramide; 0.5%-2.2% of trehalose; 0.6%-1.1% of a purslane extract; the product for treating acne comprises the following raw materials: 66%-70% of the composition, 2%-7% of tea tree essential oil, 6%-11% of tranexamic acid, 5%-9% of arbutin, 4%-7% of recombinant human epidermal growth factors and 0.4%-0.8% of a preservative and pure water, a water phase, an oil phase and active ingredients are constructed, gradient mixing is performed, and the composition containing minocycline is prepared. The composition is mixed with raw materials of a product for treating acne, and the finally prepared product can be used for treating acne, repairing scars and recovering skin vitality.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and in particular to a minocycline-containing composition and application thereof in preparing a product for treating acne. Background Art

[0002] Acne is a common skin disease caused by a variety of factors, including infection with Propionibacterium acnes, inflammation, and impaired skin barrier function. Traditional acne treatments, such as minocycline, while possessing excellent anti-inflammatory properties, suffer from poor stability and transdermal penetration. Furthermore, single-ingredient treatments often fail to fully address acne issues, such as inflammation control, scar repair, and skin rejuvenation.

[0003] In recent years, the use of plant extracts in skin treatments has garnered significant attention. For example, purslane extract (Portulaca oleracea L.) possesses anti-inflammatory and antioxidant properties, alleviating inflammation by inhibiting the NF-κB pathway. Dipotassium glycyrrhizate in licorice extract further enhances its anti-inflammatory effects by blocking COX-2. The synergistic effects of these natural ingredients offer the potential for developing novel acne treatments.

[0004] In terms of manufacturing processes, traditional high-temperature sterilization and simple mixing often lead to degradation of active ingredients, affecting product effectiveness. However, the application of nitrogen protection, low-temperature emulsification, and microencapsulation technology can effectively improve the retention and stability of active ingredients. These technological advances provide technical support for the development of highly effective and stable acne treatment products.

[0005] Therefore, the present invention aims to provide a minocycline-containing composition and its use in preparing products for treating acne, and to solve the problems existing in the prior art by optimizing the combination of ingredients and the preparation process, thereby improving the therapeutic effect and product stability. Summary of the Invention

[0006] (1) Technical problems solved

[0007] In response to the shortcomings of the existing technology, the present invention provides a minocycline-containing composition and its use in the preparation of products for treating acne. The composition has the advantages of synergistic antibacterial, highly effective anti-inflammatory and high active ingredient retention rate, and solves the problems of poor stability of traditional drugs, limited therapeutic effect of single ingredients and easy degradation of active ingredients.

[0008] (2) Technical solution

[0009] To achieve the above-mentioned object, the present invention provides the following technical solution: a minocycline-containing composition, comprising minocycline, an anti-inflammatory component, an antioxidant component, a repair component, and a moisturizing component, wherein the components of the composition and their weight ratios range as follows: minocycline 8% to 11%; allantoin 0.5% to 2%; vitamin C 5% to 9%; vitamin E 2% to 3%; collagen 3% to 7%; grape seed extract 1% to 1.5%; ceramide 0.2% to 0.5%; trehalose 0.5% to 2.2%; purslane extract 0.6% to 1.1%; and licorice extract 0.1% to 0.3%.

[0010] Preferably, the anti-inflammatory component consists of allantoin, purslane extract and licorice extract; the antioxidant component consists of vitamin C, vitamin E and grape seed extract; the repair component consists of collagen and ceramide; and the moisturizing component consists of trehalose.

[0011] Preferably, the components of the composition and their weight ratios are: minocycline 9%; allantoin 2%; vitamin C 9%; vitamin E 2.5%; collagen 6%; grape seed extract 1.3%; ceramide 0.25%; trehalose 2%; purslane extract 1%; and licorice extract 0.25%.

[0012] Preferably, the components of the composition and their weight ratios are: minocycline 8%; allantoin 0.6%; vitamin C 6%; vitamin E 3%; collagen 4%; grape seed extract 1.5%; ceramide 0.5%; trehalose 0.9%; purslane extract 0.7%; and licorice extract 0.1%.

[0013] Preferably, the components of the composition and their weight ratios are: minocycline 11%; allantoin 2%; vitamin C 9%; vitamin E 2%; collagen 7%; grape seed extract 1.1%; ceramide 0.3%; trehalose 1.9%; purslane extract 0.9%; and licorice extract 0.11%.

[0014] Preferably, the preparation process of the grape seed extract is as follows: (1) Wash, dry and crush the grape seeds, and then pass through a 60-mesh sieve to obtain grape seed powder; (2) Grape seed powder was added with 70% to 80% ethanol solution at a material-liquid ratio of 1:15-20, and refluxed at 60-65°C for 2-3 times, each time for 1.5-2 hours, and the extracts were combined; (3) The extract is concentrated under reduced pressure at a temperature of 45-50°C until it is free of ethanol, and the concentrate is collected by passing it through an AB-8 macroporous resin column to obtain a grape seed extract with a proanthocyanidin content of ≥95%.

[0015] Preferably, the preparation process of the licorice extract is: (1) Raw material processing: Licorice root slices are defatted by CO2 supercritical fluid and crushed to 80 mesh; (2) Dynamic countercurrent extraction: Add ammonia-ethanol mixed solution with a pH of 8.0-8.5 at a solid-liquid ratio of 1:12-15, and circulate the extraction at 45-50°C for 2-3 times, each time for 1-1.5 hours; (3) Acid precipitation purification: Combine the extracts, adjust the pH to 3.0-3.5 with hydrochloric acid, let stand for 10-12 hours, and centrifuge to obtain the precipitate; (4) Membrane separation and purification: The precipitate was redissolved in 75% to 80% ethanol and passed through a 0.45 μm microfiltration membrane and a 10 kDa ultrafiltration membrane to remove large molecular impurities; (5) Freeze drying: The filtrate is pre-frozen at -35 to -40°C and then freeze-dried in a vacuum to obtain a licorice extract with a glycyrrhizic acid content of ≥90%.

[0016] Preferably, the preparation method of the composition is: S1. Ingredient preparation: prepare minocycline, allantoin, purslane extract, licorice extract, vitamin C, vitamin E, grape seed extract, collagen, ceramide and trehalose in a formula ratio; S2. Phase system construction: For aqueous phase construction, stir trehalose and allantoin at 65-70°C until completely dissolved, then cool to 35-40°C, add collagen and ceramide for liposome homogenization and emulsification. For oil phase construction, ultrasonically disperse vitamin E and grape seed extract for 15-20 minutes, add purslane extract and licorice extract, and continue ultrasonically dispersing for 25-30 minutes. For active component construction, dry-mix minocycline and vitamin C in advance and pass through a 100-mesh sieve for later use. S3. Gradient mixing: add the aqueous phase dropwise into the oil phase and emulsify at high speed to form a primary emulsion. Add the active component three times under nitrogen protection. Control the temperature ≤35°C to obtain a composition for standby use.

[0017] Preferably, the preparation steps of the product for treating acne are: Step 1: prepare 66% to 70% of the composition, 2% to 7% of tea tree essential oil, 6% to 11% of tranexamic acid, 5% to 9% of arbutin, 4% to 7% of recombinant human epidermal growth factor, and 0.4% to 0.8% of a preservative, with the remaining raw material being pure water; Step 2: Heat pure water to 60-65°C, add tranexamic acid and arbutin, stir until completely dissolved, then cool to 40-45°C for later use; Step 3: Add the composition and tea tree essential oil to the solution prepared in step 2 in order, start the high-speed stirring device, and stir at a speed of 1500-2000 r / min for 30-40 minutes; Step 4: Lower the temperature to 35-40° C., add recombinant human epidermal growth factor and preservative, stir evenly, and control the pH value between 5.0-5.5 to obtain a mixed solution; Step 5: Sterilization and filling: The mixed solution is sterilized and filtered through a 0.22 μm polyethersulfone filter membrane, vacuum degassed, and filled into a light-proof vacuum pump bottle, which is then filled with nitrogen and sealed. Step 6. Quality Control: Test the content of active ingredients. The labeled amount of minocycline is 98% to 100%, and the retention rates of vitamin C and vitamin E are both ≥98%.

[0018] A minocycline-containing composition is used in preparing a product for treating acne. The acne-treating product prepared according to the minocycline-containing composition is used for treating acne, repairing scars and restoring skin vitality.

[0019] Compared with the prior art, the present invention provides a minocycline-containing composition and its use in preparing a product for treating acne, which has the following beneficial effects: 1. The present invention achieves the beneficial effect of reducing the MIC90 value of Propionibacterium acnes through the synergistic antibacterial effect of minocycline, tea tree essential oil, and allantoin. Among them, minocycline has the effect of inhibiting Propionibacterium acnes, while tea tree essential oil and allantoin can further enhance the antibacterial effect. Tea tree essential oil can destroy bacterial biofilms, and allantoin can inhibit the production of inflammatory factors, thereby acting synergistically. The minimum inhibitory concentration of the three combinations on Propionibacterium acnes is reduced, the antibacterial ability is stronger, and more powerful support is provided for the treatment of acne.

[0020] 2. The present invention achieves the beneficial effect of improving the IL-6 inhibition rate through the dual anti-inflammatory mechanism of purslane extract and dipotassium glycyrrhizate. Among them, purslane extract can inhibit the NF-κB (nuclear factor κB) pathway, while dipotassium glycyrrhizate can block COX-2 (cyclooxygenase-2). The two work synergistically to effectively inhibit the production of the inflammatory factor IL-6. The combination of the two has the best anti-inflammatory effect and can better alleviate the problem of acne skin inflammation, thereby alleviating the symptoms of skin redness and swelling.

[0021] 3. The preparation process of the present invention achieves the beneficial effect of high active ingredient retention rate through nitrogen protection, low-temperature emulsification process and microencapsulation technology. Among them, the application of low-temperature emulsification process can effectively avoid the problem of degradation of vitamin C active ingredients caused by traditional processes such as high-temperature sterilization. The high active ingredient retention rate can ensure that the composition can still exert good therapeutic effects during long-term storage and use, thereby improving the storage stability of the product and the reliability of treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a step diagram of the present invention. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] See also Figure 1 A minocycline-containing composition is composed of minocycline, an anti-inflammatory component, an antioxidant component, a repair component and a moisturizing component. The components of the composition and their weight ratio range are: minocycline 8% to 11%; allantoin 0.5% to 2%; vitamin C 5% to 9%; vitamin E 2% to 3%; collagen 3% to 7%; grape seed extract 1% to 1.5%; ceramide 0.2% to 0.5%; trehalose 0.5% to 2.2%; purslane extract 0.6% to 1.1%; and licorice extract 0.1% to 0.3%.

[0025] The function table of its ingredients is as follows: Table 1

[0026] Specifically, the anti-inflammatory component is composed of allantoin, purslane extract and licorice extract; the antioxidant component is composed of vitamin C, vitamin E and grape seed extract; the repair component is composed of collagen and ceramide; and the moisturizing component is composed of trehalose.

[0027] The advantage is that the present invention achieves the beneficial effect of a high IL-6 inhibition rate through the dual anti-inflammatory mechanism of purslane extract and dipotassium glycyrrhizate, wherein purslane extract can inhibit the NF-κB (nuclear factor κB, a protein complex whose main function is to control transcription, cell survival, immune response and inflammatory response) pathway, while dipotassium glycyrrhizate can block COX-2 (cyclooxygenase-2, an enzyme that is mainly involved in inflammatory response and transmission of pain signals). The two act synergistically to effectively inhibit the production of the inflammatory factor IL-6. The combination of the two has the best anti-inflammatory effect, can better alleviate the problem of acne skin inflammation, and thus relieve the symptoms of skin redness and swelling.

[0028] Specifically, the preparation process of grape seed extract is as follows: (1) Wash, dry and crush the grape seeds, and then pass through a 60-mesh sieve to obtain grape seed powder; (2) Grape seed powder was added with 70% to 80% ethanol solution at a material-liquid ratio of 1:15-20, and refluxed at 60-65°C for 2-3 times, each time for 1.5-2 hours, and the extracts were combined; (3) The extract is concentrated under reduced pressure at a temperature of 45-50°C until it is free of ethanol, and the concentrate is collected by passing it through an AB-8 macroporous resin column to obtain a grape seed extract with a proanthocyanidin content of ≥95%.

[0029] Specifically, the preparation process of licorice extract is as follows: (1) Raw material processing: Licorice root slices are defatted by CO2 supercritical fluid and crushed to 80 mesh; (2) Dynamic countercurrent extraction: Add ammonia-ethanol mixed solution with a pH of 8.0-8.5 at a solid-liquid ratio of 1:12-15, and circulate the extraction at 45-50°C for 2-3 times, each time for 1-1.5 hours; (3) Acid precipitation purification: Combine the extracts, adjust the pH to 3.0-3.5 with hydrochloric acid, let stand for 10-12 hours, and centrifuge to obtain the precipitate; (4) Membrane separation and purification: The precipitate was redissolved in 75% to 80% ethanol and passed through a 0.45 μm microfiltration membrane and a 10 kDa ultrafiltration membrane to remove large molecular impurities; (5) Freeze drying: The filtrate is pre-frozen at -35 to -40°C and then freeze-dried in a vacuum to obtain a licorice extract with a glycyrrhizic acid content of ≥90%.

[0030] Specifically, the preparation method of the composition is: S1. Ingredient preparation: prepare minocycline, allantoin, purslane extract, licorice extract, vitamin C, vitamin E, grape seed extract, collagen, ceramide and trehalose in a formula ratio; S2. Phase system construction: For aqueous phase construction, stir trehalose and allantoin at 65-70°C until completely dissolved, then cool to 35-40°C, add collagen and ceramide for liposome homogenization and emulsification. For oil phase construction, ultrasonically disperse vitamin E and grape seed extract for 15-20 minutes, add purslane extract and licorice extract, and continue ultrasonically dispersing for 25-30 minutes. For active component construction, dry-mix minocycline and vitamin C in advance and pass through a 100-mesh sieve for later use. S3. Gradient mixing: add the aqueous phase dropwise into the oil phase and emulsify at high speed to form a primary emulsion. Add the active component three times under nitrogen protection. Control the temperature ≤35°C to obtain a composition for standby use.

[0031] Specifically, the preparation steps of the product for treating acne are as follows: Step 1: Prepare 66% to 70% of the composition, 2% to 7% of tea tree essential oil, 6% to 11% of tranexamic acid, 5% to 9% of arbutin, 4% to 7% of recombinant human epidermal growth factor, and 0.4% to 0.8% of a preservative. The remaining raw material is pure water. By precisely proportioning the ingredients, the resulting product can be ensured to have comprehensive therapeutic effects. The composition provides basic antibacterial, anti-inflammatory, repair, and moisturizing functions; tea tree essential oil enhances antibacterial properties; tranexamic acid and arbutin help whiten the skin and inhibit pigmentation; recombinant human epidermal growth factor promotes skin repair; and preservatives ensure product stability. This scientific ratio can lay the foundation for the subsequent preparation of efficient and stable acne treatment products. Step 2: Heat pure water to 60-65°C, add tranexamic acid and arbutin. This temperature helps tranexamic acid and arbutin dissolve quickly, improves dissolution efficiency, and avoids uneven distribution of components due to incomplete dissolution. Stir until completely dissolved, then cool to 40-45°C for later use. Cooling can ensure the dissolution effect and avoid the damage of subsequent ingredients to high temperature, providing a suitable temperature environment for subsequent steps and ensuring that the activity of each ingredient is retained. Step 3: Add the composition and tea tree essential oil to the solution prepared in step 2 in sequence, start the high-speed stirring device, and stir at a speed of 1500-2000 r / min for 30-40 minutes to fully mix the ingredients and form a stable emulsified system. High-speed stirring can break the interfacial tension between the ingredients, so that the ingredients are evenly dispersed in the solution, ensuring that the ingredients can act evenly on the skin during use and exert a synergistic therapeutic effect. Step 4: Lower the temperature to 35-40°C, add recombinant human epidermal growth factor and preservatives. At this temperature, the high temperature can effectively prevent the activity of the recombinant human epidermal growth factor from being destroyed, while ensuring that the preservatives are evenly distributed. Stir evenly and control the pH value between 5.0 and 5.5 to obtain a mixed solution. This pH value is close to the pH of human skin, which can reduce skin irritation, improve product safety, make the product more suitable for long-term use on the skin, and also promote the stability and effectiveness of each ingredient. Step 5. Sterilization and filling: The mixed solution is sterilized and filtered through a 0.22 μm polyethersulfone filter membrane, and after vacuum degassing, it is filled into a light-proof vacuum pump bottle and sealed with nitrogen. This step can effectively remove bacteria, molds and other microorganisms in the mixed solution, ensure the sterility of the product, and avoid the risk of skin infection during use. Vacuum degassing can remove bubbles in the solution and prevent bubbles from forming gaps in the product, affecting the stability and appearance of the product. Filling into a light-proof vacuum pump bottle and sealing with nitrogen can effectively isolate air and light, prevent oxidation and degradation of the active ingredients in the product, and extend the shelf life of the product. At the same time, the use of a vacuum pump bottle is also convenient for users to take, avoiding secondary contamination of the product during use; Step 6. Quality Control: Test the active ingredient content. The minocycline content is 98% to 100% of the labeled amount, and the retention rates of vitamin C and vitamin E are both ≥ 98%. This step can guarantee the effectiveness of the product, ensure that each batch of products meets the standards, and provide users with safe and effective acne treatment products.

[0032] Specifically, a minocycline-containing composition is used in the preparation of a product for treating acne. The acne-treating product prepared according to the above-mentioned minocycline-containing composition is used for treating acne, repairing scars and restoring skin vitality.

[0033] The advantages are: through the synergistic antibacterial effect of minocycline, tea tree essential oil and allantoin, the beneficial effect of reducing the MIC90 value of Propionibacterium acnes is achieved. Among them, minocycline has the effect of inhibiting Propionibacterium acnes, while tea tree essential oil and allantoin can further enhance the antibacterial effect. Tea tree essential oil can destroy bacterial biofilms, and allantoin can inhibit the production of inflammatory factors, thereby acting synergistically. The minimum inhibitory concentration of the three combinations on Propionibacterium acnes is reduced, the antibacterial ability is stronger, and more powerful support is provided for the treatment of acne.

[0034] Example 1 Specifically, the components of the composition and their weight ratios are: minocycline 9%; allantoin 2%; vitamin C 9%; vitamin E 2.5%; collagen 6%; grape seed extract 1.3%; ceramide 0.25%; trehalose 2%; purslane extract 1%; and licorice extract 0.25%.

[0035] Example 2 Specifically, the components of the composition and their weight ratios are: minocycline 8%; allantoin 0.6%; vitamin C 6%; vitamin E 3%; collagen 4%; grape seed extract 1.5%; ceramide 0.5%; trehalose 0.9%; purslane extract 0.7%; and licorice extract 0.1%.

[0036] Example 3 Specifically, the components of the composition and their weight ratios are: minocycline 11%; allantoin 2%; vitamin C 9%; vitamin E 2%; collagen 7%; grape seed extract 1.1%; ceramide 0.3%; trehalose 1.9%; purslane extract 0.9%; and licorice extract 0.11%.

[0037] The examples of the present invention were all prepared by the method of the present invention. To verify the practicality of the method of the present invention, five existing acne treatment products were compared with the finished products prepared by the examples of the present invention. The comparative data are shown in Table 2 below: Table 2

[0038] From the analysis in Table 2, it can be seen that the MIC90 values ​​of Examples 1-3 (0.06-0.12 μg / mL) are significantly lower than those of existing products (0.18-0.30 μg / mL), which is attributed to the synergistic antibacterial effect of minocycline, tea tree essential oil and allantoin (destroying bacterial biofilm + inhibiting inflammatory factors); the IL-6 inhibition rate is as high as 91.7% (Example 3), which is due to the dual anti-inflammatory mechanism of Portulaca oleracea extract (inhibiting NF-κB pathway) and dipotassium glycyrrhizate (blocking COX-2); the scar repair rate of Example 3 (53.1%) is 1.8 times that of the control group (29.8%), which is due to the dual effects of rhEGF promoting collagen regeneration and tranexamic acid inhibiting fibrosis; the active ingredient retention rate is ≥96 % (Examples 1-3), due to nitrogen protection, low-temperature emulsification process and microencapsulation technology, while existing products suffer from severe vitamin C degradation due to high-temperature sterilization (retention rate ≤ 88.7%); erythema index ≤ 1.2 (Examples 1-3), verifying the low-irritation design of tannin-free grape seed extract and CO2 supercritical defatted licorice extract; Example 3 has a clinical efficacy of 94.1% (p<0.01), proving that the combined efficacy of the composition in controlling acne, repairing and revitalizing is significantly better than that of a single antibiotic (existing product A has an efficacy of 68.5%).

[0039] The advantages are: during the preparation process of the present invention, nitrogen protection, low-temperature emulsification process and microencapsulation technology are used to achieve the beneficial effect of a high active ingredient retention rate. Among them, the application of the low-temperature emulsification process can effectively avoid the problem of degradation of the active ingredient of vitamin C caused by traditional processes such as high-temperature sterilization. Under the conditions of 40°C / 3 months, the active ingredient retention rate of the present invention is ≥96%, while the existing product suffers from severe degradation of vitamin C due to high-temperature sterilization, and the retention rate is ≤88.7%. The high active ingredient retention rate ensures that the composition can still exert a good therapeutic effect during long-term storage and use, thereby improving the stability and reliability of the product.

[0040] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A minocycline-containing composition, characterized in that The composition consists of minocycline, an anti-inflammatory component, an antioxidant component, a repair component and a moisturizing component. The components of the composition and their weight ratio range are: minocycline 8% to 11%; allantoin 0.5% to 2%; vitamin C 5% to 9%; vitamin E 2% to 3%; collagen 3% to 7%; grape seed extract 1% to 1.5%; ceramide 0.2% to 0.5%; trehalose 0.5% to 2.2%; purslane extract 0.6% to 1.1%; and licorice extract 0.1% to 0.3%.

2. A minocycline-containing composition according to claim 1, characterized in that: The anti-inflammatory component consists of allantoin, purslane extract and licorice extract; the antioxidant component consists of vitamin C, vitamin E and grape seed extract; the repair component consists of collagen and ceramide; and the moisturizing component consists of trehalose.

3. A minocycline-containing composition according to claim 1, characterized in that: The components of the composition and their weight ratios are: minocycline 9%, allantoin 2%, vitamin C 9%, vitamin E 2.5%, collagen 6%, grape seed extract 1.3%, ceramide 0.25%, trehalose 2%, purslane extract 1%, and licorice extract 0.25%.

4. A minocycline-containing composition according to claim 1, characterized in that: The components of the composition and their weight ratios are: minocycline 8%, allantoin 0.6%, vitamin C 6%, vitamin E 3%, collagen 4%, grape seed extract 1.5%, ceramide 0.5%, trehalose 0.9%, purslane extract 0.7%, and licorice extract 0.1%.

5. A minocycline-containing composition according to claim 1, characterized in that The components of the composition and their weight ratios are: minocycline 11%, allantoin 2%, vitamin C 9%, vitamin E 2%, collagen 7%, grape seed extract 1.1%, ceramide 0.3%, trehalose 1.9%, purslane extract 0.9%, and licorice extract 0.11%.

6. A minocycline-containing composition according to claim 1, characterized in that: The preparation process of the grape seed extract is as follows: (1) Wash, dry and crush the grape seeds, and then pass through a 60-mesh sieve to obtain grape seed powder; (2) Grape seed powder was added with 70% to 80% ethanol solution at a material-liquid ratio of 1:15-20, and refluxed at 60-65°C for 2-3 times, each time for 1.5-2 hours, and the extracts were combined; (3) The extract is concentrated under reduced pressure at a temperature of 45-50°C until it is free of ethanol, and the concentrate is collected by passing it through an AB-8 macroporous resin column to obtain a grape seed extract with a proanthocyanidin content of ≥95%.

7. The minocycline-containing composition according to claim 1, wherein: The preparation process of the licorice extract is as follows: (1) Raw material processing: Licorice root slices are defatted by CO2 supercritical fluid and crushed to 80 mesh; (2) Dynamic countercurrent extraction: Add ammonia-ethanol mixed solution with a pH of 8.0-8.5 at a solid-liquid ratio of 1:12-15, and circulate the extraction at 45-50°C for 2-3 times, each time for 1-1.5 hours; (3) Acid precipitation purification: Combine the extracts, adjust the pH to 3.0-3.5 with hydrochloric acid, let stand for 10-12 hours, and centrifuge to obtain the precipitate; (4) Membrane separation and purification: The precipitate was redissolved in 75% to 80% ethanol and passed through a 0.45 μm microfiltration membrane and a 10 kDa ultrafiltration membrane to remove large molecular impurities; (5) Freeze drying: The filtrate is pre-frozen at -35 to -40°C and then freeze-dried in a vacuum to obtain a licorice extract with a glycyrrhizic acid content of ≥90%.

8. The minocycline-containing composition according to claim 1, characterized in that The preparation method of the composition is: S1. Ingredient preparation: prepare minocycline, allantoin, purslane extract, licorice extract, vitamin C, vitamin E, grape seed extract, collagen, ceramide and trehalose in a formula ratio; S2. Phase system construction: For aqueous phase construction, stir trehalose and allantoin at 65-70°C until completely dissolved, then cool to 35-40°C, add collagen and ceramide for liposome homogenization and emulsification. For oil phase construction, ultrasonically disperse vitamin E and grape seed extract for 15-20 minutes, add purslane extract and licorice extract, and continue ultrasonically dispersing for 25-30 minutes. For active component construction, dry-mix minocycline and vitamin C in advance and pass through a 100-mesh sieve for later use. S3. Gradient mixing: add the aqueous phase dropwise into the oil phase and emulsify at high speed to form a primary emulsion. Add the active component three times under nitrogen protection. Control the temperature ≤35°C to obtain a composition for standby use.

9. The minocycline-containing composition according to claim 1, wherein: The preparation steps of the product for treating acne are: Step 1: prepare 66% to 70% of the composition, 2% to 7% of tea tree essential oil, 6% to 11% of tranexamic acid, 5% to 9% of arbutin, 4% to 7% of recombinant human epidermal growth factor, and 0.4% to 0.8% of a preservative, with the remaining raw material being pure water; Step 2: Heat pure water to 60-65°C, add tranexamic acid and arbutin, stir until completely dissolved, then cool to 40-45°C for later use; Step 3: Add the composition and tea tree essential oil to the solution prepared in step 2 in order, start the high-speed stirring device, and stir at a speed of 1500-2000 r / min for 30-40 minutes; Step 4: Lower the temperature to 35-40° C., add recombinant human epidermal growth factor and preservative, stir evenly, and control the pH value between 5.0-5.5 to obtain a mixed solution; Step 5: Sterilization and filling: The mixed solution is sterilized and filtered through a 0.22 μm polyethersulfone filter membrane, vacuum degassed, and filled into a light-proof vacuum pump bottle, which is then filled with nitrogen and sealed. Step 6. Quality Control: Test the content of active ingredients. The labeled amount of minocycline is 98% to 100%, and the retention rates of vitamin C and vitamin E are both ≥98%.

10. Use of a minocycline-containing composition in preparing a product for treating acne, characterized in that: The acne treatment product prepared from the minocycline-containing composition according to claim 9 is used for treating acne, repairing scars and restoring skin vitality.

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