Active probiotic component detection equipment

Abstract

The invention relates to the technical field of microbiological detection, in particular to active probiotic component detection equipment which comprises a box body, a microscope is arranged in the box body, a glass slide is arranged below the microscope, a cover glass is placed at the top of the glass slide, a box door is rotationally connected to one side of the box body through a hinge, a placement plate is fixedly mounted in the box body, and sliding grooves are formed in the two sides of the placement plate. A clamping unit is slidably connected in the sliding groove, a glass slide is fixedly connected above the placing plate through the clamping unit, a first mounting groove is formed in one end, close to the glass slide, of the placing plate, a driving unit is fixedly connected in the first mounting groove, a separating unit is arranged above the glass slide, and a heating unit is arranged below the glass slide; the separation unit and the heating unit are both controlled by a driving unit, the bottom end of the clamping unit penetrates through the placing plate to be fixedly connected with a moving unit, a dyeing unit is arranged in the end, away from the clamping unit, of the placing plate, and an angle adjusting unit is arranged below the dyeing unit.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, in particular to active probiotic component detection equipment. Background Art

[0002] Active probiotics refer to a class of active microorganisms that are beneficial to human health. They usually exist in the intestines and can regulate the balance of intestinal flora, enhance immunity or promote nutrient absorption. Common ones include bifidobacteria and lactobacilli. The efficacy of probiotics is highly dependent on the number of live bacteria and specific strains. Different strains have obvious differences in their effects. Testing is needed to confirm whether the product contains the labeled strains to avoid using cheap strains to impersonate patented strains. At the same time, pathogenic bacteria contamination such as Salmonella and Staphylococcus aureus should be excluded to prevent safety hazards.

[0003] Therefore, people will dissolve and sample probiotic products and then conduct preliminary observations of the probiotics under a microscope. The activity and type of the strain can be determined by observing the motility of the living microbial cells, and the number of bacteria can be directly calculated. When the species type cannot be determined by the motility and habits of the living microbial cells or further careful observation is required to determine the species type, the sample needs to be Gram-stained. The Gram staining method generally includes four steps: primary staining, mordanting, decolorization, and re-staining. Manual operation is relatively cumbersome and inefficient. Summary of the Invention

[0004] In response to the above situation, the present invention provides an active probiotic component detection device: after preliminarily observing the motility of the probiotics, the cover glass can be automatically removed, the slide can be heated and fixed, and then Gram staining can be performed, thereby facilitating the user to observe and judge the components of the probiotics, and allowing the user to perform other work during the staining period, which can effectively improve the user's work efficiency and thus enhance the user experience.

[0005] The present invention provides an active probiotic component detection device, which includes a box body, a microscope is arranged in the box body, a slide is arranged under the microscope, a cover glass is placed on the top of the slide, one side of the box body is connected to the box door by a hinge, a placement plate is fixedly installed in the box body, two sides of the placement plate are provided with slide grooves, a clamping unit is slidably connected in the slide grooves, the top of the placement plate is fixedly connected to the slide through the clamping unit, a first installation groove is provided in one end of the placement plate close to the slide, a driving unit is fixedly connected in the first installation groove, a separation unit capable of separating the cover glass is provided above the slide, and a separation unit is provided below the slide. A heating unit is provided that can heat the glass slide to fix the bacteria species. The separation unit and the heating unit are both controlled by a driving unit. The bottom end of the clamping unit is fixedly connected to a moving unit through the placement plate. The moving unit can control the position of the glass slide by pulling the clamping unit to move. A dyeing unit that can dye the probiotics in the glass slide is provided in the end of the placement plate away from the clamping unit. An angle adjustment unit that can control the angle of the glass slide during rinsing is provided below the dyeing unit. A water sink is provided on the placement plate below the dyeing unit, and a waste water tank is provided below the water sink. The waste water tank is slidably installed in the box body.

[0006] Preferably, the clamping unit includes an L-shaped support column, four of which are provided, and the four support columns are symmetrically slidably installed in the sliding grooves opened on both sides of the placement plate in a group of two. A movable column is provided at one end of the support column, and the movable column is a hollow structure. A first spring is provided in the movable column, one end of the first spring is fixedly connected to the inner wall of the movable column, and the other end is fixedly connected to the support column. The end of the movable column away from the support column is fixedly connected to a rotating column, and the other end of the rotating column is rotatably connected to a clamping block, and the clamping block clamps the slide through the thrust of the first spring, and the bottom end of the support column is fixedly connected to a connecting column, and a group of support columns are fixedly connected through the connecting column.

[0007] Preferably, the driving unit includes a first motor, which is fixedly installed in the first installation groove. The output end of the first motor is fixedly connected to a threaded rod, the surface of the threaded rod is threadedly connected to a driving column, and the driving column is slidably installed in the placement plate.

[0008] Preferably, the separation unit includes a light baffle, one end of the light baffle is fixedly connected to a driving column, the light baffle, a movable block is slidably connected to the middle of the light baffle, the bottom end of the movable block is fixedly connected to a suction cup, the outer wall of the movable block is fixedly connected to a movable plate, the movable plate passes through the placement plate and is slidably installed in the first installation groove, the bottom end of the movable plate is slidably connected to the first special-shaped groove, the first special-shaped groove is opened on the inner wall of the first installation groove, a collection box is provided under the suction cup, and the collection box is slidably installed in the box body.

[0009] Preferably, the heating unit includes a second mounting groove, which is opened in the placement plate, and the lighting lamp and the heating lamp are slidably installed in the second mounting groove. The lighting lamp and the heating lamp are fixedly connected to a cylinder on a side close to the first mounting groove, and both ends of the cylinder are slidably connected to a second special-shaped groove, which is opened on the inner wall of the first mounting groove. The cylinders of the lighting lamp and the heating lamp pass through the second special-shaped groove and are sleeved with a synchronization block at one end. The synchronization block is provided with a limiting groove at one end close to the cylinder, and a limiting block is slidably connected in the limiting groove. The limiting block is fixedly connected to the outer wall of the cylinder, and a driving column is fixedly connected to the outer wall of the synchronization block at one end.

[0010] Preferably, the moving unit includes an electric telescopic rod, a pair of which are symmetrically installed below both sides of the placement plate, and the telescopic ends of the pair of electric telescopic rods are fixedly connected to push blocks, an oblique slide groove is provided in the push block, a moving column is slidably installed in the slide groove, the moving column passes through the connecting column and is slidably connected to an L-shaped guide groove, the guide groove is fixedly connected to the inner wall of the box, a limit groove is provided above the guide groove, the limit groove is provided in the placement plate, a pair of limit blocks are slidably connected in the limit groove, and one end of the limit block passes through the limit groove and is slidably connected to the support column.

[0011] Preferably, the dyeing unit includes a second motor, which is fixedly installed in the placement plate, and the output end of the second motor is fixedly connected to a worm, one side of the worm is rotatably connected to a worm wheel, and the worm wheel is coaxially fixedly connected to a rotating column, the bottom end of the rotating column passes through the worm wheel and is rotatably installed in the placement plate, and the top of the rotating column passes through the placement plate and is fixedly connected to a turntable, and nine dyeing tanks are fixedly installed in the turntable, and the nine dyeing tanks are respectively filled with crystal violet dye, clean water, iodine solution dye, clean water, ethanol decolorant, clean water, sand yellow dye, clean water, and cedar oil. The water outlet of the dyeing tank passes through the turntable and is fixedly connected to a solenoid valve, and an air pipe is provided below the solenoid valve, and the air pipe is fixedly installed on the top of the placement plate, and an air port is opened in the air pipe near the radial direction of the slide.

[0012] Preferably, the angle adjustment unit includes a driving disk, which is coaxially fixedly connected to the rotating column. A third special-shaped groove is provided in the driving disk. The third special-shaped groove is M-shaped, and the bottom end corresponds to the position of the staining tank filled with clean water. A lifting block is slidably connected in the third special-shaped groove. When the staining tank filled with clean water rotates to above the glass slide, the lifting block is located at the bottom end of the third special-shaped groove. Both ends of the lifting block are fixedly connected to the lifting column. The top of the lifting column is fixedly connected to a slider. The slider is slidably installed in the placement plate. The end of the slider away from the lifting column is fixedly connected to a clamping column. A lifting unit that can be clamped with the clamping column is provided on one side of the clamping column.

[0013] Preferably, the lifting unit includes a receiving slot and a compensation slot. The receiving slot is opened in the support column close to the lifting column. A second spring is fixedly installed in the receiving slot. A telescopic column is fixedly connected to the top of the second spring. The telescopic column is slidably installed in the receiving slot. A clamping slot is opened on one side of the telescopic column close to the clamping column, and the clamping slot can be clamped with the clamping column. The compensation slot is opened at the bottom end of the support column away from the lifting column. A third spring is fixedly installed in the compensation slot. One end of the third spring is fixedly connected to the compensation slot, and the other end is fixedly connected to the support column. The support column is slidably installed in the compensation slot through the third spring.

[0014] The beneficial effects of the above technical solution are: (1) The present invention can perform preliminary observation of probiotic components and then automatically stain them for further detailed observation. In addition, a heating unit is provided to dry and fix the probiotics on the slide using the temperature of a baking lamp without using an open flame, which can effectively improve the drying effect and safety. (2) The present invention can control the height and position of the slide by setting a moving unit, so that the slide can be moved away from the microscope to the staining unit for staining, which can effectively avoid affecting the microscope during staining, and can automatically send the stained slide to the bottom of the microscope, thereby facilitating the user to observe and determine the type of probiotics; (3) The present invention arranges the observation port of the microscope and the slide glass in the box body, and can remove the cover glass through the separation unit in the box body, so that the entire staining process can be completed in the box body, thereby avoiding contamination of the slide glass by external microorganisms during staining, which can effectively improve the detection effect and user experience. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic structural diagram of the active probiotic component detection equipment of the present invention; Figure 2 This is a schematic diagram of the internal structure of the active probiotic component detection device of the present invention; Figure 3 This is a schematic structural diagram of the driving unit and separation unit of the active probiotic component detection device of the present invention; Figure 4 This is an enlarged schematic diagram of the structure of the active probiotic component detection device A of the present invention; Figure 5 This is a schematic diagram of the internal structure of the separation unit and the heating unit of the active probiotic component detection equipment of the present invention; Figure 6 This is a schematic diagram of the internal structure of the heating unit of the active probiotic component detection equipment of the present invention; Figure 7 This is a schematic diagram of the structure of the dyeing unit of the active probiotic component detection equipment of the present invention; Figure 8This is an enlarged schematic diagram of the structure of the active probiotic component detection device at position B of the present invention; Figure 9 This is a schematic structural diagram of the dyeing unit and angle adjustment unit of the active probiotic component detection equipment of the present invention; Figure 10 This is an enlarged schematic diagram of the structure of the active probiotic component detection device at position C of the present invention; Figure 11 This is a front view of the dyeing unit and angle adjustment unit of the active probiotic component detection device of the present invention; Figure 12 This is an enlarged schematic diagram of the structure of location C of the active probiotic component detection device of the present invention.

[0016] Explanation of the numbers in the figure: 1. Box body; 12. Microscope; 13. Box door; 14. Placement plate; 15. Slide; 16. Slide; 17. Cover glass; 18. First mounting slot; 19. Sink; 101. Waste water tank; 2. Clamping unit; 21. Support column; 22. Movable column; 23. First spring; 24. Rotating column; 25. Clamping block; 26. Connecting column; 3. Driving unit; 31. First motor; 32. Threaded rod; 33. Driving column; 4. Separating unit; 41. Light baffle; 42. Movable block; 43. Suction cup; 44. Moving plate; 45. First special-shaped slot; 46. Collecting box; 5. Heating unit; 51. Second mounting slot; 52. Illuminating lamp; 53. Bake Lamp; 54. Second special-shaped groove; 55. Synchronizing block; 56. Cylinder; 6. Moving unit; 61. Electric telescopic rod; 62. Push block; 63. Moving column; 64. Guide groove; 65. Limiting groove; 66. Limiting block; 71. Second motor; 72. Worm; 73. Worm gear; 74. Rotating column; 75. Turntable; 76. Dyeing tank; 77. Solenoid valve; 78. Air pipe; 79. Air port; 8. Angle adjustment unit; 81. Drive disk; 82. Third special-shaped groove; 83. Lifting block; 84. Lifting column; 85. Slider; 86. Clamping column; 87. Storage slot; 88. Second spring; 89. Telescopic column; 801. Clamping slot; 802. Compensating slot; 803. Third spring. DETAILED DESCRIPTION

[0017] The above and other technical contents, features and effects of the present invention are described below with reference to the attached Figures 1 to 11 It can be clearly presented in the detailed description of the embodiments that the structural contents mentioned in the following embodiments are all referenced to the drawings in the specification.

[0018] Figure 1 and Figure 2The active probiotic component detection device is schematically shown, including a box body 1, a microscope 12 is arranged in the box body 1, a slide 16 is arranged under the microscope 12, a cover glass 17 is placed on the top of the slide 16, a box door 13 is connected to one side of the box body 1 by a hinge, a placement plate 14 is fixedly installed in the box body 1, a slide 15 is provided on both sides of the placement plate 14, a clamping unit 2 is slidably connected in the slide 15, a slide glass 16 is fixedly connected to the top of the placement plate 14 through the clamping unit 2, a first mounting groove 18 is provided in one end of the placement plate 14 close to the slide glass 16, a driving unit 3 is fixedly connected in the first mounting groove 18, a separation unit 4 capable of separating the cover glass 17 is provided above the slide glass 16, and the slide glass 17 is fixedly connected to the top of the slide glass 16. A heating unit 5 is provided below the slide 16, which can heat the slide 16 to fix the bacteria. The separation unit 4 and the heating unit 5 are both controlled by the driving unit 3. The bottom end of the clamping unit 2 is fixedly connected to the moving unit 6 through the placement plate 14. The moving unit 6 can control the position of the slide 16 by pulling the clamping unit 2 to move. A dyeing unit 7 is provided at the end of the placement plate 14 away from the clamping unit 2, which can dye the probiotics in the slide 16. An angle adjustment unit 8 is provided below the dyeing unit 7, which can control the angle of the slide 16 during rinsing. A water sink 19 is provided on the placement plate 14 below the dyeing unit 7, and a waste water tank 101 is provided below the water sink 19. The waste water tank 101 is slidably installed in the box body 1.

[0019] In a specific implementation, it is only necessary to place the device in a suitable place, then open the box door 13 and place the sampled slide 16 in the clamping unit 2, and then the probiotics on the slide 16 can be observed through the microscope 12. At this time, the probiotics are not stained, and the user can clearly observe the movement state and life habits of the probiotics, avoiding the death of the probiotics after staining and the inability to observe the probiotics' movement and life habits. After the preliminary observation of the probiotics or when further observation is required, the driving unit 3 can be started, and the driving unit 3 can drive the separation unit 4 to move the slide 16 The cover glass 17 on the glass slide 16 is removed and collected, and the lighting lamp 52 of the heating unit 5 is switched to the baking lamp 53. At this time, the moving unit 6 is started. The moving unit 6 will first lower the height of the glass slide 16, thereby facilitating the baking lamp 53 to solidify the probiotics on the glass slide 16. When the probiotics on the glass slide 16 are solidified, the moving unit 6 can drive the glass slide 16 to move horizontally, and then the glass slide 16 is moved to the bottom of the dyeing unit 7, and then the dyeing unit 7 dyes the probiotics on the glass slide 16, and the wastewater generated during dyeing will flow into the wastewater tank 101 along the water trough 19.

[0020] like Figure 4 、 Figure 5 and Figure 7The clamping unit 2 shown includes an L-shaped support column 21, and there are four support columns 21. The four support columns 21 are symmetrically slidably installed in the slide grooves 15 opened on both sides of the placement plate 14 in pairs. A movable column 22 is sleeved on one end of the support column 21. The movable column 22 is a hollow structure. A first spring 23 is provided in the movable column 22. One end of the first spring 23 is fixedly connected to the inner wall of the movable column 22, and the other end is fixedly connected to the support column 21. The end of the movable column 22 away from the support column 21 is fixedly connected to a rotating column 24, and the other end of the rotating column 24 is rotatably connected to a clamping block 25. The clamping block 25 clamps the slide 16 by the thrust of the first spring 23. The bottom end of the support column 21 is fixedly connected to a connecting column 26, and a group of support columns 21 are fixedly connected by the connecting column 26.

[0021] In a specific implementation, it is only necessary to place the glass slide 16 on the clamping block 25 . At this time, the first spring 23 will push the movable column 22 to move, and the movement of the movable column 22 will drive the clamping block 25 to move, thereby enabling the clamping block 25 to clamp and fix the glass slide 16 .

[0022] like Figure 2 and Figure 3 The drive unit 3 shown includes a first motor 31, which is fixedly installed in the first installation groove 18. The output end of the first motor 31 is fixedly connected to a threaded rod 32, and the surface of the threaded rod 32 is threadedly connected to a drive column 33, which is slidably installed in the placement plate 14.

[0023] In a specific implementation, when the first motor 31 rotates, it drives the threaded rod 32 to rotate, and the rotation of the threaded rod 32 pushes the driving column 33 to slide in the placement plate 14, thereby providing power to the separation unit 4 and the heating unit 5.

[0024] like Figure 3 and Figure 5 The separation unit 4 shown includes a light baffle 41, one end of which is fixedly connected to the driving column 33, the light baffle 41, and a movable block 42 slidably connected in the middle of the light baffle 41, the bottom end of the movable block 42 is fixedly connected to the suction cup 43, the outer wall of the movable block 42 is fixedly connected to the movable plate 44, the movable plate 44 passes through the placement plate 14 and is slidably installed in the first installation groove 18, the bottom end of the movable plate 44 is slidably connected to the first special-shaped groove 45, the first special-shaped groove 45 is opened on the inner wall of the first installation groove 18, a collection box 46 is provided under the suction cup 43, and the collection box 46 is slidably installed in the box body 1.

[0025] In a specific implementation, when the first motor 31 rotates through the threaded rod 32 to push the driving column 33 to move, the movement of the driving column 33 will drive the light baffle 41 to move, and the movement of the light baffle 41 will drive the suction cup 43 to move and drive the moving plate 44 to slide in the first special-shaped groove 45. When the suction cup 43 moves above the cover glass 17, the moving plate 44 will also move downward along the curvature of the first special-shaped groove 45, and the downward movement of the moving plate 44 will drive the movable block 42 to move downward, and the downward movement of the movable block 42 will drive the suction cup 43 to move downward and adhere to the cover glass 17. At this time, starting the suction cup 43 can adsorb the cover glass 17, and at this time, reversing the first motor 31 will cause the screw to rotate. The grooved rod 32 is reversed and drives the driving column 33 to move toward the collection box 46, thereby driving the suction cup 43 to retreat and driving the heating unit 5 to the center, and then the slide 16 is moved close to the heating unit 5 through the moving unit 6 for drying and fixing. At this time, the light baffle 41 is located directly below the microscope 12, which can effectively prevent the light source of the baking lamp from entering the microscope 12 and causing damage. When the drying and fixing is completed, the first motor 31 can be reversed. The reversal of the first motor 31 will drive the driving column 33 to reset, and then drive the suction cup 43 to reset. At this time, closing the suction cup 43 can allow the cover glass 17 to fall into the collection box 46. When the collection box 46 is full, the collection box 46 can be pulled out by holding the handle.

[0026] like Figure 3 、 Figure 5 and Figure 6 The heating unit 5 shown includes a second mounting groove 51, which is opened in the placement plate 14, and a lighting lamp 52 and a heating lamp 53 are slidably installed in the second mounting groove 51. The lighting lamp 52 and the heating lamp 53 are fixedly connected to a cylinder 56 on a side close to the first mounting groove 18, and both ends of the cylinder 56 are slidably connected to a second special-shaped groove 54, which is opened on the inner wall of the first mounting groove 18. The cylinder 56 of the lighting lamp 52 and the heating lamp 53 passes through one end of the second special-shaped groove 54 and is sleeved with a synchronization block 55. The synchronization block 55 is provided with a limiting groove 57 on one end close to the cylinder 56, and a limiting block 58 is slidably connected in the limiting groove 57. The limiting block 58 is fixedly connected to the outer wall of the cylinder 56, and the outer wall of one end of the synchronization block 55 is fixedly connected to the driving column 33.

[0027] In a specific implementation, when the driving column 33 moves, it will drive the synchronization block 55 to move, and the movement of the synchronization block 55 will drive the cylinder 56 of the lighting lamp 52 and the baking lamp 53 to slide in the second special-shaped groove 54. When the suction cup 43 moves to the top of the slide glass 16 and removes the cover glass 17, the positions of the lighting lamp 52 and the baking lamp 53 are adjusted by controlling the forward and reverse rotation of the first motor 31. When the baking lamp 53 needs to be used for drying, it is only necessary to drive the first motor 31 to drive the threaded rod 32 to rotate, and then the threaded rod 32 pushes the driving column 33 to move, and then pushes the baking lamp 53 to the slide glass 16. 6, thereby completing the light source switching, so that the heating unit 5 can heat and fix the probiotics on the slide 16, and the middle part of the second special-shaped groove 54 is a convex slide groove, which can make the lamp mouth of the baking lamp 53 move upward a certain distance to make up for the wall thickness of the second installation groove 51, thereby improving the drying efficiency, and the cylinder 56 is slidably installed in the limiting groove 57 through the limiting block 58, so that the cylinder 56 can only move up and down along the vertical direction of the synchronization block 55 and cannot rotate, thereby making the lighting lamp 52 and the baking lamp 53 able to slide horizontally in the second special-shaped groove 54.

[0028] like Figure 4 、 Figure 7 and Figure 8 The shown moving unit 6 includes an electric telescopic rod 61, and a pair of electric telescopic rods 61 are symmetrically installed under the two sides of the placement plate 14. The telescopic ends of the pair of electric telescopic rods 61 are fixedly connected to push blocks 62, and an oblique sliding groove is opened in the push block 62. A moving column 63 is slidably installed in the sliding groove. The moving column 63 passes through the connecting column 26 and is slidably connected to an L-shaped guide groove 64. The guide groove 64 is fixedly connected to the inner wall of the box body 1. A limit groove 65 is provided above the guide groove 64. The limit groove 65 is opened in the placement plate 14. A pair of limit blocks 66 are slidably connected in the limit groove 65. One end of the limit block 66 passes through the limit groove 65 and is slidably connected to the support column 21.

[0029] In a specific implementation, when the separation unit 4 removes the cover glass 17, the electric telescopic rod 61 can be started. The contraction of the electric telescopic rod 61 will drive the push block 62 to move, and the movement of the push block 62 will press down the moving column 63, thereby causing the moving column 63 to slide downward in the guide groove 64. When the moving column 63 is at the bottom end of the push block 62, the contraction can be stopped. Because the moving column 63 is fixedly connected to the connecting column 26 of a group of support columns 21, the movement of the moving column 63 can drive the support column 21 to move, and the movement of the support column 21 can drive the slide 16 to move, thereby causing the electric telescopic rod 61 to contract. The telescopic rod 61 can control the distance between the glass slide 16 and the baking lamp 53, so that the glass slide 16 can fully contact the heat source of the baking lamp 53, so that the probiotics on the glass slide 16 can be solidified on the glass slide 16. When the probiotics on the glass slide 16 are completely solidified, the electric telescopic rod 61 can be retracted. At this time, because the moving column 63 is located at the bottom end of the push block 62, there is no downward pressure space, so that the push block 62 can pull the moving column 63 to slide parallel to the guide groove 64. When the glass slide 16 moves to above the water tank 19, the electric telescopic rod 61 can be closed.

[0030] like Figure 7 、 Figure 9 and Figure 11 The dyeing unit 7 shown includes a second motor 71, which is fixedly installed in the placement plate 14. The output end of the second motor 71 is fixedly connected to a worm 72, and one side of the worm 72 is rotatably connected to a worm gear 73. The worm gear 73 is coaxially fixedly connected to a rotating column 74. The bottom end of the rotating column 74 passes through the worm gear 73 and is rotatably installed in the placement plate 14. The top of the rotating column 74 passes through the placement plate 14 and is fixedly connected to a turntable 75. Nine dyeing tanks 76 are fixedly installed in the turntable 75. The top of the nine dyeing tanks 76 is provided with a water inlet. The water inlet has a threaded inner portion. It is connected to a ventilation plug of model M10, which can prevent water from flowing out without affecting the entry of air into the staining tank 76. The nine staining tanks 76 are respectively filled with crystal violet stain, clean water, iodine solution stain, clean water, ethanol decolorizer, clean water, sand yellow stain, clean water, and cedar oil. The water outlet of the staining tank 76 is fixedly connected to a solenoid valve 77 through the turntable 75. An air pipe 78 is provided below the solenoid valve 77. The air pipe 78 is fixedly installed on the top of the placement plate 14, and an air port 79 is opened on the air pipe 78 near the radial direction of the slide 16.

[0031] The second motor 71 is started after the electric telescopic rod 61 moves the glass slide 16 to above the water tank 19. The rotation of the second motor 71 can drive the worm 72 to rotate, and the rotation of the worm 72 will drive the worm gear 73 to rotate, and the rotation of the worm gear 73 will drive the rotating column 74 to rotate, and the rotation of the rotating column 74 will drive the turntable 75 to rotate, and the rotation of the turntable 75 will drive the dyeing tank 76 to rotate. When the water outlet of the dyeing tank 76 is aligned with the glass slide 16, the solenoid valve 77 can be opened, so that the crystal violet dye in the dyeing tank 76 can drip onto the glass slide 16, thereby dyeing the probiotics on the glass slide 16. After one minute, the second motor 71 is started again, and the dyeing tank 76 filled with clean water is rotated above the glass slide 16. At this time, the angle adjustment unit 8 can tilt the glass slide 16. Open the solenoid valve 77 of the clean water staining tank 76 to rinse the glass slide 16 with clean water, thereby washing away the crystal violet staining solution. After washing away the crystal violet staining solution and starting the external heater, the heater blows air into the trachea 78, and the warm air in the trachea 78 flows out from the air port 79, thereby air-drying the glass slide 16. Then rotate the turntable 75 again to move the staining tank 76 containing iodine solution stain to the top of the glass slide 16 for staining. This cycle continues until the sand yellow stain is used. After the sand yellow stain is rinsed away, the staining tank 76 containing cedar oil can be rotated to the top of the glass slide 16 and cedar oil can be dripped onto the stained probiotics on the glass slide 16 to complete the staining. At this time, the glass slide 16 is returned to the bottom of the microscope 12 by the mobile unit 6 and can be observed again to determine the composition of the probiotics.

[0032] like Figure 4 、 Figure 9 、 Figure 10 、 Figure 11 and Figure 12 The angle adjustment unit 8 shown includes a driving disk 81, which is coaxially fixedly connected to the rotating column 74. A third special-shaped groove 82 is provided in the driving disk 81. The third special-shaped groove 82 is M-shaped, and the bottom end corresponds to the position of the staining tank 76 filled with clean water. A lifting block 83 is slidably connected in the third special-shaped groove 82. When the staining tank 76 filled with clean water rotates to above the slide 16, the lifting block 83 is located at the bottom end of the third special-shaped groove 82. Both ends of the lifting block 83 are fixedly connected to the lifting column 84. The top of the lifting column 84 is fixedly connected to a slider 85. The slider 85 is slidably installed in the placement plate 14. The end of the slider 85 away from the lifting column 84 is fixedly connected to a clamping column 86. One side of the clamping column 86 is provided with a lifting unit that can be clamped with the clamping column 86.

[0033] When the second motor 71 drives the rotating column 74 through the worm gear 72 and the worm gear 73, the driving disk 81 will be driven to rotate, and the rotation of the driving disk 81 will drive the lifting block 83 to slide in the third special-shaped groove 82, thereby causing the lifting block 83 to move up and down along the arc of the third special-shaped groove 82, and the lifting block 83 will drive the lifting column 84 to move up and down, and the lifting column 84 will drive the slider 85 and the clamping column 86 to move up and down, which will drive the clamping column 86 to move up and down, and the slide 16 will be tilted through the lifting unit, so that when the staining tank 76 filled with clean water rotates to above the slide 16, the slide 16 can be tilted, thereby better cleaning the slide 16.

[0034] like Figure 4 、 Figure 9 、 Figure 10 、 Figure 11 and Figure 12 The lifting unit includes a receiving slot 87 and a compensation slot 802. The receiving slot 87 is opened in the support column 21 near the lifting column 84. A second spring 88 is fixedly installed in the receiving slot 87. A telescopic column 89 is fixedly connected to the top of the second spring 88. The telescopic column 89 is slidably installed in the receiving slot 87. A card slot 801 is opened on the side of the telescopic column 89 close to the card column 86. The card slot 801 can be engaged with the card column 86. The compensation slot 802 is opened at the bottom end of the support column 21 away from the lifting column 84. A third spring 803 is fixedly installed in the compensation slot 802. One end of the third spring 803 is fixedly connected to the compensation slot 802, and the other end is fixedly connected to the support column 21. The support column 21 is slidably installed in the compensation slot 802 through the third spring 803.

[0035] When the lifting block 83 drives the clamping column 86 to move up and down, the clamping column 86 is engaged with the clamping slot 801, and the clamping column 86 moves up and down, which can drive the clamping slot 801 to move up and down. The up and down movement of the clamping slot 801 will drive the telescopic column 89 to move in the storage slot 87, and the top of the telescopic column 89 is fixedly connected to the support column 21, so that when the telescopic column 89 moves downward, the height of the support column 21 close to the dyeing unit 7 in a group of support columns 21 can be lowered, and the height drop of one support column 21 in a group of support columns 21 will cause the clamping block 25 to tilt, and the tilting of the clamping block 25 will drive the slide 16 to tilt, so that the dyeing tank 76 filled with clean water can better clean the slide 16. Because the height of one support column 21 drops and the width of the clamping block 25 remains unchanged, the support column 21 with unchanged height will move toward the support column 21 with lowered height, and then slide in the compensation groove 802 and compress the third spring 803, so that the slide 16 tilts.

[0036] In actual use, it is only necessary to place the device in a suitable place, then open the box door 13 and place the sampled slide 16 on the clamping block 25. At this time, the first spring 23 will push the movable column 22 to move, and the movement of the movable column 22 will drive the clamping block 25 to move, so that the clamping block 25 can clamp and fix the slide 16, and the probiotics on the slide 16 can be observed through the microscope 12. At this time, the probiotics are not stained, and the user can clearly observe the movement state and life habits of the probiotics, avoiding the death of the probiotics after staining and the inability to observe the motility and life habits of the probiotics. After the preliminary observation of the probiotics or when further observation is needed, the first motor 31 can be started. When the first motor 31 rotates, it will drive the threaded rod 32 to rotate, and the screw The rotation of the threaded rod 32 will push the driving column 33 to slide in the placement plate 14, and the movement of the driving column 33 will drive the light blocking plate 41 to move, and the movement of the light blocking plate 41 will drive the suction cup 43 to move and drive the moving plate 44 to slide in the first special-shaped groove 45. When the suction cup 43 moves above the cover glass 17, the moving plate 44 will also move downward along the arc of the first special-shaped groove 45, and the downward movement of the moving plate 44 will drive the movable block 42 to move downward, and the downward movement of the movable block 42 will drive the suction cup 43 to move downward and adhere to the cover glass 17. At this time, the suction cup 43 can be started to adsorb the cover glass 17. At this time, the first motor 31 is reversed, and the threaded rod 32 is reversed to drive the driving column 33 to move toward the collection box 46, thereby driving the suction cup 43 to retreat. When the drying and fixing is completed, the first motor 31 can be reversed. The reversal of the first motor 31 will drive the driving column 33 to reset, and then drive the suction cup 43 to reset. At this time, closing the suction cup 43 can make the cover glass 17 fall into the collection box 46. When the collection box 46 is full, the collection box 46 can be pulled out by holding the handle, and the movement of the driving column 33 will also drive the synchronization block 55 to move, and the movement of the synchronization block 55 will drive the lighting lamp 52 and the cylinder 56 of the baking lamp 53 to slide in the second special-shaped groove 54. When the suction cup 43 moves to After the cover glass 17 is removed from the top of the glass slide 16, the positions of the lighting lamp 52 and the baking lamp 53 are adjusted by controlling the forward and reverse rotation of the first motor 31. When the baking lamp 53 needs to be used for drying, it is only necessary to drive the first motor 31 to drive the threaded rod 32 to rotate, and then the threaded rod 32 pushes the driving column 33 to move, and then pushes the baking lamp 53 to the bottom of the glass slide 16, thereby completing the light source switching, so that the heating unit 5 can heat and fix the probiotics on the glass slide 16, and the middle part of the second special-shaped groove 54 is a raised sliding groove, which can make the lamp mouth of the baking lamp 53 move upward a certain distance to make up for the wall thickness of the second installation groove 51, thereby improving the drying efficiency, and the cylinder 56 is slidably installed in the limiting groove 57 through the limiting block 58.The cylinder 56 can only move up and down along the vertical direction of the synchronization block 55 and cannot rotate, thereby allowing the lighting lamp 52 and the baking lamp 53 to slide horizontally in the second special-shaped groove 54. When the separation unit 4 removes the cover glass 17, the electric telescopic rod 61 can be started. The contraction of the electric telescopic rod 61 will drive the push block 62 to move, and the movement of the push block 62 will press down the moving column 63, thereby causing the moving column 63 to slide downward in the guide groove 64. When the moving column 63 is at the bottom end of the push block 62, the contraction can be stopped. Because the moving column 63 is fixedly connected to the connecting column 26 of a group of support columns 21, the movement of the moving column 63 can drive the support column 21 to move, and the movement of the support column 21 can drive the slide 16 to move, thereby making the electric telescopic rod 61 The distance between the glass slide 16 and the baking lamp 53 can be controlled, so that the glass slide 16 can fully contact the heat source of the baking lamp 53, so that the probiotics on the glass slide 16 can be cured on the glass slide 16. When the probiotics on the glass slide 16 are completely cured, the electric telescopic rod 61 can be retracted. At this time, because the moving column 63 is located at the bottom end of the push block 62, there is no downward pressure space, so that the push block 62 can pull the moving column 63 to slide parallel to the guide groove 64. When the glass slide 16 moves to above the water tank 19, the electric telescopic rod 61 can be closed, and the second motor 71 can be started at this time. The rotation of the second motor 71 can drive the worm 72 to rotate, and the rotation of the worm 72 will drive the worm gear 73 to rotate, and the rotation of the worm gear 73 will drive the rotating column 74 to rotate. The rotation of the rotating column 74 drives the turntable 75 to rotate, and the rotation of the turntable 75 drives the dyeing tank 76 to rotate. When the water outlet of the dyeing tank 76 is aligned with the slide glass 16, the electromagnetic valve 77 can be opened, so that the crystal violet dye in the dyeing tank 76 can drip onto the slide glass 16, thereby dyeing the probiotics on the slide glass 16. One minute later, the second motor 71 is started again, and the dyeing tank 76 filled with clean water is rotated to above the slide glass 16. At this time, the angle adjustment unit 8 can tilt the slide glass 16. At this time, the electromagnetic valve 77 of the clean water dyeing tank 76 is opened to allow the clean water to rinse the slide glass 16, thereby flushing away the crystal violet dye. The external heater can be started to make the heater blow to the air pipe 7 8, and the warm air in the trachea 78 will flow out from the air port 79, thereby air-drying the glass slide 16, and then the turntable 75 is rotated again to move the dyeing tank 76 filled with iodine solution dye to the top of the glass slide 16 for dyeing, and this cycle continues until the sand yellow dye is used. After the sand yellow dye is rinsed off, the turntable 75 can be rotated to the dyeing tank 76 filled with cedar oil and rotated to the top of the glass slide 16 to drip cedar oil on the dyed probiotics on the glass slide 16 to complete the dyeing. At this time, the glass slide 16 is returned to the bottom of the microscope 12 by the moving unit 6 and can be observed again to determine the composition of the probiotics. When the electric telescopic rod 61 moves the glass slide 16 to the top of the water tank 19, the clamping column 86 can be clamped with the clamping slot 801 in the lifting unit.At this time, the second motor 71 drives the rotating column 74 to rotate through the worm 72 and the worm gear 73, which drives the driving plate 81 to rotate. The rotation of the driving plate 81 drives the lifting block 83 to slide in the third special-shaped groove 82, thereby making the lifting block 83 move up and down along the arc of the third special-shaped groove 82. The up and down movement of the lifting block 83 drives the lifting column 84 to move up and down, and the up and down movement of the lifting column 84 drives the slider 85 and the card column 86 to move up and down, which drives the card column 86 to move up and down, and the up and down movement of the card column 86 can drive the card slot 801 to move up and down, and the up and down movement of the card slot 801 can drive the telescopic column 89 to move in the storage slot 87, and the top of the telescopic column 89 is fixedly connected to the support column 21, so that the telescopic column 89 can move downward. At this time, the height of the support column 21 in the group of support columns 21 close to the dyeing unit 7 decreases, and the height decrease of one support column 21 in the group of support columns 21 causes the clamping block 25 to tilt, and the tilting of the clamping block 25 drives the slide glass 16 to tilt, thereby allowing the dyeing tank 76 filled with clean water to better clean the slide glass 16. Because the height of one support column 21 decreases while the width of the clamping block 25 remains unchanged, the support column 21 with the unchanged height will move toward the support column 21 with the decreased height, thereby sliding in the compensation groove 802 and compressing the third spring 803, causing the slide glass 16 to tilt. When the dyeing tank 76 filled with clean water rotates to above the slide glass 16, the slide glass 16 can tilt, thereby better cleaning the slide glass 16.

Claims

1. An active probiotic component detection device, comprising a housing (1), a microscope (12) disposed in the housing (1), a glass slide (16) disposed below the microscope (12), a cover glass (17) disposed on top of the glass slide (16), and characterized in that: One side of the box body (1) is connected to a box door (13) by a hinge, a placement plate (14) is fixedly installed in the box body (1), a slide groove (15) is provided on both sides of the placement plate (14), a clamping unit (2) is slidably connected in the slide groove (15), a glass slide (16) is fixedly connected above the placement plate (14) through the clamping unit (2), a first installation groove (18) is provided in one end of the placement plate (14) close to the glass slide (16), a driving unit (3) is fixedly connected in the first installation groove (18), a separation unit (4) capable of separating a cover glass (17) is provided above the glass slide (16), a heating unit (5) capable of heating the glass slide (16) to fix the bacterial strain is provided below the glass slide (16), and the separation unit ( 4) and the heating unit (5) are controlled by the driving unit (3), the bottom end of the clamping unit (2) is fixedly connected to the moving unit (6) through the placement plate (14), and the moving unit (6) can control the position of the slide glass (16) by pulling the clamping unit (2) to move, and a dyeing unit (7) capable of dyeing the probiotics in the slide glass (16) is provided in the end of the placement plate (14) away from the clamping unit (2), and an angle adjustment unit (8) capable of controlling the angle of the slide glass (16) during washing is provided below the dyeing unit (7), and a water tank (19) is provided below the placement plate (14) at the bottom of the dyeing unit (7), and a waste water tank (101) is provided below the water tank (19), and the waste water tank (101) is slidably installed in the box body (1).

2. The active probiotic component detection device according to claim 1, characterized in that: The clamping unit (2) includes an L-shaped support column (21), and the support columns (21) are provided with four. The four support columns (21) are symmetrically slidably installed in the slide grooves (15) opened on both sides of the placement plate (14) in pairs. A movable column (22) is provided at one end of the support column (21). The movable column (22) is a hollow structure. A first spring (23) is provided in the movable column (22). One end of the first spring (23) is fixedly connected to the inner wall of the movable column (22), and the other end is fixedly connected to the support column (21). The end of the movable column (22) away from the support column (21) is fixedly connected to the rotating column (24). The other end of the rotating column (24) is rotatably connected to the clamping block (25). The clamping block (25) clamps the slide (16) through the thrust of the first spring (23). The bottom end of the support column (21) is fixedly connected to the connecting column (26). A group of support columns (21) are fixedly connected through the connecting column (26).

3. The active probiotic component detection device according to claim 1, characterized in that: The drive unit (3) includes a first motor (31), the first motor (31) is fixedly installed in the first installation groove (18), the output end of the first motor (31) is fixedly connected to a threaded rod (32), the surface of the threaded rod (32) is threadedly connected to a drive column (33), and the drive column (33) is slidably installed in the placement plate (14).

4. The active probiotic component detection device according to claim 1 or 3, characterized in that: The separation unit (4) includes a light baffle (41), one end of the light baffle (41) is fixedly connected to the driving column (33), the light baffle (41), the middle of the light baffle (41) is slidably connected to a movable block (42), the bottom end of the movable block (42) is fixedly connected to a suction cup (43), the outer wall of the movable block (42) is fixedly connected to a movable plate (44), the movable plate (44) passes through the placement plate (14) and is slidably installed in the first installation groove (18), the bottom end of the movable plate (44) is slidably connected to a first special-shaped groove (45), the first special-shaped groove (45) is opened on the inner wall of the first installation groove (18), a collection box (46) is provided below the suction cup (43), and the collection box (46) is slidably installed in the box body (1).

5. The active probiotic component detection device according to claim 1, characterized in that: The heating unit (5) includes a second mounting groove (51), the second mounting groove (51) is opened in the placement plate (14), and a lighting lamp (52) and a heating lamp (53) are slidably installed in the second mounting groove (51). The lighting lamp (52) and the heating lamp (53) are fixedly connected to a cylinder (56) on one side close to the first mounting groove (18), and the two ends of the cylinder (56) are slidably connected to a second special-shaped groove (54), and the second special-shaped groove (54) is opened on the inner wall of the first mounting groove (18). The cylinders (56) of the lighting lamp (52) and the heating lamp (53) pass through one end of the second special-shaped groove (54) and are sleeved with a synchronization block (55). The synchronization block (55) is opened at one end close to the cylinder (56) and is provided with a limiting groove (57). A limiting block (58) is slidably connected in the limiting groove (57), and the limiting block (58) is fixedly connected to the outer wall of the cylinder (56). The outer wall of one end of the synchronization block (55) is fixedly connected to the driving column (33).

6. The active probiotic component detection device according to claims 1, 2, 3 and 4, characterized in that: The mobile unit (6) includes an electric telescopic rod (61), which is provided with a pair of electric telescopic rods (61). The pair of electric telescopic rods (61) are symmetrically installed below the two sides of the placement plate (14). The telescopic ends of the pair of electric telescopic rods (61) are fixedly connected to push blocks (62). An oblique sliding groove is provided in the push block (62). A moving column (63) is slidably installed in the sliding groove. The moving column (63) passes through the connecting column (26) and is slidably connected to an L-shaped guide groove (64). The guide groove (64) is fixedly connected to the inner wall of the box body (1). A limiting groove (65) is provided above the guide groove (64). The limiting groove (65) is provided in the placement plate (14). A pair of limiting blocks (66) are slidably connected in the limiting groove (65). One end of the limiting block (66) passes through the limiting groove (65) and is slidably connected to the support column (21).

7. The active probiotic component detection device according to claims 1, 2, 3 and 4, characterized in that: The dyeing unit (7) includes a second motor (71), the second motor (71) is fixedly installed in the placement plate (14), the output end of the second motor (71) is fixedly connected to a worm (72), one side of the worm (72) is rotatably connected to a worm wheel (73), the worm wheel (73) is coaxially fixedly connected to a rotating column (74), the bottom end of the rotating column (74) passes through the worm wheel (73) and is rotatably installed in the placement plate (14), the top end of the rotating column (74) passes through the placement plate (14) and is fixedly connected to a turntable (75), the turntable (7 5) Nine staining tanks (76) are fixedly installed inside, and the nine staining tanks (76) are respectively filled with crystal violet stain, clean water, iodine solution stain, clean water, ethanol decolorant, clean water, sand yellow stain, clean water, and cedar oil. The water outlet of the staining tank (76) passes through the turntable (75) and is fixedly connected to a solenoid valve (77). An air pipe (78) is provided below the solenoid valve (77). The air pipe (78) is fixedly installed on the top of the placement plate (14). The air pipe (78) is provided with an air port (79) near the radial direction of the slide (16).

8. The active probiotic component detection device according to claims 1, 2, 3 and 4, characterized in that: The angle adjustment unit (8) includes a driving disk (81), which is coaxially fixedly connected to the rotating column (74). A third special-shaped groove (82) is provided in the driving disk (81), and the third special-shaped groove (82) is M-shaped, and the bottom end corresponds to the position of the staining tank (76) filled with clean water. A lifting block (83) is slidably connected in the third special-shaped groove (82). When the staining tank (76) filled with clean water rotates to above the slide glass (16), the lifting block (83) is located at the bottom end of the third special-shaped groove (82). Both ends of the lifting block (83) are fixedly connected to the lifting column (84). The top of the lifting column (84) is fixedly connected to a slider (85). The slider (85) is slidably installed in the placement plate (14). The end of the slider (85) away from the lifting column (84) is fixedly connected to a clamping column (86). One side of the clamping column (86) is provided with a lifting unit that can be clamped with the clamping column (86).

9. The active probiotic component detection device according to claims 1, 2, 3 and 4, characterized in that: The lifting unit includes a receiving slot (87) and a compensation slot (802). The receiving slot (87) is opened in the support column (21) close to the lifting column (84). A second spring (88) is fixedly installed in the receiving slot (87). The top of the second spring (88) is fixedly connected to a telescopic column (89). The telescopic column (89) is slidably installed in the receiving slot (87). A clamping slot (801) is opened on one side of the telescopic column (89) close to the clamping column (86). The clamping slot (801) can be clamped with the clamping column (86). The compensation slot (802) is opened at the bottom end of the support column (21) away from the lifting column (84). A third spring (803) is fixedly installed in the compensation slot (802). One end of the third spring (803) is fixedly connected to the compensation slot (802), and the other end is fixedly connected to the support column (21). The support column (21) is slidably installed in the compensation slot (802) through the third spring (803).

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