Steaming processing technology of rhizoma polygoni cuspidati
Through expansion treatment and composite microcapsule technology, the problem of easy oxidation and degradation of saponin components of button seven was solved, the stability of the medicinal material and the content of rare saponins were improved, and its application range was expanded.
Patent Information
- Application Number
- CN202510872290.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-26
- Publication Date
- 2025-09-23
AI Technical Summary
The saponin components in Kouziqi are easily oxidized and degraded, and have poor stability, which affects its medicinal value and application range.
The puffing treatment is combined with composite microcapsule technology, and the button seven medicinal materials are processed through a twin-screw extrusion puffing machine to form a porous structure. Composite microcapsules are composed of salvia miltiorrhiza extract, astragalus extract, kudzu root extract, tea polyphenols, β-cyclodextrin and citric acid to form a stable microcapsule system to encapsulate saponin components and prevent oxidative degradation.
The content and stability of rare saponins in the Kouziqi medicinal material are improved, the processing efficiency and stability of the medicinal material are enhanced, and its application range is expanded.
Smart Images
Figure BDA0005470156060000141 
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of traditional Chinese medicine, and in particular to a steaming and processing process of Kouziqi. Background Art
[0002] Knotweed, also known as pearl ginseng, large-leaf Panax notoginseng, button seven, lump seven, and pan seven, has high medicinal value. It is the best of the 72 "seven" medicines in Tujia medicine and is known as the "Tujia holy medicine". It is bitter, sweet, and slightly cold in nature. It enters the liver, lung, and stomach meridians. It has the effects of nourishing the lungs and yin, removing blood stasis and relieving pain, and stopping bleeding. Modern pharmacological research shows that Knotweed has multiple pharmacological activities such as anti-tumor, anti-inflammatory and analgesic, antioxidant, cardiovascular and cerebrovascular protection, and liver protection.
[0003] Processing refers to the process of making unprocessed Chinese medicinal materials of Kouziqi into medicinal slices for clinical use through cleaning, cutting, frying, calcining, roasting, roasting, soaking, soaking, steaming and boiling under the guidance of traditional Chinese medicine theory. After processing, the material basis of Kouziqi changes, which can significantly increase the saponin content, but the saponin components in Kouziqi are easily oxidized and degraded, and have poor stability. Summary of the Invention
[0004] The invention provides a steaming and processing process for Kouziqi, which solves the problem that the saponin components of Kouziqi are easily oxidized and degraded and have poor stability during the steaming and processing process of Kouziqi.
[0005] The technical solution of the present invention:
[0006] A steaming and preparing process of Button Seven comprises the following steps:
[0007] S1. After removing impurities and cleaning the buttons, the buttons are puffed and dried to obtain puffed buttons.
[0008] S2. The puffed buttons seven herbs, composite microcapsules and water were mixed and stirred evenly. After 1-4h of incubation at 30-40 ℃, the mixture was steamed and dried to obtain steamed buttons seven herbs.
[0009] The composite microcapsule is made of a composite material as a core material and β-cyclodextrin and citric acid as capsule materials;
[0010] The composite material is obtained by mixing salvia miltiorrhiza extract, astragalus root extract, kudzu root extract and tea polyphenols.
[0011] Furthermore, in step S1, the impurity removal and cleaning are specifically as follows: the button seven medicinal materials are subjected to impurity removal, and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed, and then the surface mud and sand are removed by washing with water.
[0012] Furthermore, in step S1, the puffing treatment adopts a twin-screw extruder, the barrel temperature is 60-80°C, the screw speed is 200-220r / min, the feed rate is 10-12kg / h, and the amount of deionized water added is 45-55% of the weight of the cleaned button seven medicinal material.
[0013] Furthermore, in step S2, the mass ratio of the expanded Kouziqi medicinal material, composite microcapsules and water is (80-100):(10-15):(20-30).
[0014] Furthermore, in step S2, the steaming temperature is 115-130°C, and the steaming time is 1-4 hours; the drying temperature is 60-90°C, and the drying time is 5-10 minutes.
[0015] Furthermore, in step S2, the composite microcapsules are prepared by the following steps:
[0016] A1. Salvia miltiorrhiza extract, Astragalus extract, and Pueraria root extract were added to ethanol and stirred at 700-800 r / min for 20-30 min. Tea polyphenols were added and stirred for 20-30 min. The mixture was stirred at 40-50 ° C until the solvent evaporated and dried to obtain a composite material.
[0017] A2. β-cyclodextrin was added to deionized water and stirred at 35-45°C for 30-40 minutes to obtain a β-cyclodextrin solution. The composite material and citric acid were added to deionized water and stirred at 35-45°C for 30-40 minutes to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and ultrasonically treated. The mixture was cooled to room temperature, and the solid was filtered, washed, and freeze-dried to obtain composite microcapsules.
[0018] Furthermore, during the above-mentioned A1 reaction process, salvia miltiorrhiza extract, astragalus extract, kudzu root extract and tea polyphenols are mixed. The phenolic hydroxyl groups contained in the tea polyphenols can be combined with the active groups in the salvia miltiorrhiza extract, astragalus extract and kudzu root extract through hydrogen bonds, so that the salvia miltiorrhiza extract, astragalus extract and kudzu root extract are cross-linked to form a stable system, which is conducive to the encapsulation of β-cyclodextrin. In addition, tea polyphenols have an antioxidant effect and can significantly scavenge DPPH free radicals, prevent the thermal decomposition of saponins, and have a synergistic effect with the kudzu root extract in inhibiting the oxidative degradation of saponin components.
[0019] Furthermore, in the above-mentioned A2 reaction process, the composite material is used as the core material, and β-cyclodextrin and citric acid are used as capsule materials to form microcapsules. The tea polyphenols in the composite material can be embedded in the hydrophobic cavity of β-CD, and the fat-soluble components in the Salvia miltiorrhiza extract can also interact with β-cyclodextrin, so that β-cyclodextrin encapsulates the composite material. In addition, citric acid, as a cross-linking agent, can combine with tea polyphenols and β-cyclodextrin through chemical bonds, thereby increasing the cross-linking density of the microcapsules, thereby increasing the encapsulation rate, and forming composite microcapsules.
[0020] Furthermore, in step A1, the mass ratio of the salvia miltiorrhiza extract, astragalus root extract, kudzu root extract, ethanol, and tea polyphenols is (2.2-2.4):(2.1-2.3):(1.4-1.6):(30-40):(3.5-3.9).
[0021] Furthermore, in step A2, the mass ratio of β-cyclodextrin to deionized water is (8-12):(45-55).
[0022] Furthermore, in step A2, the mass ratio of the composite material, citric acid and deionized water is (3.2-3.4):(1-1.2):(25-35).
[0023] Furthermore, in step A2, the ultrasonic treatment temperature is 40-60° C., the ultrasonic treatment frequency is 40-50 KHz, and the ultrasonic treatment time is 30-40 min.
[0024] The present invention has the following beneficial effects:
[0025] (1) In the technical solution of the present invention, the button seven medicinal material is puffed. Due to the action of high temperature, high pressure and high shear force, especially when the pressure is released, the air in the button seven medicinal material is discharged, which destroys the cell wall and cell membrane structure of the button seven medicinal material, making the button seven medicinal material surface have a porous structure, which is conducive to the release of saponin components in the button seven medicinal material. Its porous structure increases the contact area with the composite microcapsule, making it easier for the composite microcapsule to enter the interior of the button seven medicinal material, thereby improving the processing efficiency, increasing the content of rare saponins, reducing the gelatinization of starch and improving the stability of the steamed button seven medicinal material.
[0026] (2) In the technical solution of the present invention, the fat-soluble components in the Salvia miltiorrhiza extract can promote the dissolution and retention of saponin components in the Herba Lycopodii, reduce the loss of components during the processing process, and increase the content of rare saponins; the surface activity of the Astragalus extract can optimize the infiltration effect during the processing of the Herba Lycopodii, increase the moisturizing effect of the Herba Lycopodii, and help to increase the content of rare saponins in the Herba Lycopodii steamed products; the Pueraria root extract can inhibit the oxidative degradation of saponin components, improve the stability of the Herba Lycopodii steamed products, and avoid the loss of components during the processing process.
[0027] (3) In the technical solution of the present invention, salvia miltiorrhiza extract, astragalus extract, kudzu root extract and tea polyphenols are mixed. The phenolic hydroxyl groups contained in the tea polyphenols can be combined with the active groups in the salvia miltiorrhiza extract, astragalus extract and kudzu root extract through hydrogen bonds, so that the salvia miltiorrhiza extract, astragalus extract and kudzu root extract are cross-linked to form a stable system, which is conducive to the encapsulation of β-cyclodextrin. In addition, tea polyphenols have an antioxidant effect and can significantly scavenge DPPH free radicals, prevent the thermal decomposition of saponins, and have a synergistic effect with the kudzu root extract in inhibiting the oxidative degradation of saponin components.
[0028] (4) In the technical solution of the present invention, a composite material is used as a core material, and β-cyclodextrin and citric acid are used as capsule materials to form microcapsules. On the one hand, the stability of the composite material is improved to prevent the composite material from being decomposed by heat during the processing of the button seven medicinal material, thereby affecting the saponin content and effective ingredients of the button seven medicinal material steamed product. On the other hand, the formed microcapsules have a small size effect. The surface of the puffed button seven medicinal material contains a large number of pore structures, which enables the microcapsules to enter the puffed button seven medicinal material, thereby improving the processing efficiency and increasing the rare saponin content. In addition, during the processing of the puffed button seven medicinal material, the internal moisture evaporates rapidly, the outer layer dries and shrinks, and stress is generated, which causes the composite microcapsules entering the puffed button seven medicinal material to rupture, releasing the composite material, which plays a role inside the button seven medicinal material, thereby improving the processing efficiency.
[0029] (5) In the technical solution of the present invention, the β-cyclodextrin produced by the rupture of the composite microcapsules can encapsulate the saponin components, thereby improving the stability of the steamed products of the button seven medicinal material, and citric acid can inhibit the gelatinization of starch, thereby preventing the starch from gelatinizing easily, encapsulating the saponin components in the button seven medicinal material, forming a physical barrier, and reducing the dissolution rate of the saponins. The present invention mainly adopts the methods of moistening, steaming, and drying to prepare the button seven medicinal material. The preparation process is simple and the effect is excellent. Moreover, by steaming the compound microcapsules, the content of rare saponins in the steamed button seven medicinal material is increased, thereby expanding the application range of the button seven medicinal material. DETAILED DESCRIPTION
[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.
[0031] The raw materials used in the examples of the present invention are as follows, and all reagents used are of analytical grade.
[0032] Among them, the medicinal material Kouziqi was identified by Associate Professor Gong Limin of Hunan University of Chinese Medicine as the dried rhizome of P.japonicus CAMey.var.bipinnatifidus(Seem.)CYWu et KMFeng.
[0033] Salvia miltiorrhiza extract was purchased from Hebei Pengyu Biotechnology Co., Ltd.
[0034] Astragalus extract (catalog number: ZB-51251456) was purchased from Xi'an Zebang Biotechnology Co., Ltd.
[0035] Pueraria root extract (catalog number: 1524) was purchased from Xi'an Prius Bioengineering Co., Ltd.
[0036] β-Cyclodextrin was purchased from Zhengzhou Chongyuan Science and Technology Trading Co., Ltd.
[0037] Example 1
[0038] A steaming and preparing process of Button Seven comprises the following steps:
[0039] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0040] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly. After being simmered at 30 ℃ for 1h, the mixture was steamed and dried to obtain steamed button seven herbs.
[0041] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 60°C, a screw speed of 200 r / min, a feed rate of 10 kg / h, and an amount of deionized water added of 45% of the weight of the washed buckle seven medicinal material;
[0042] In step S2, the mass ratio of the expanded buckle seven medicinal materials, composite microcapsules and water is 80:10:20;
[0043] In step S2, steaming is performed in a steamer at a temperature of 115° C. for 1 hour; and drying is performed at a temperature of 60° C. for 5 minutes.
[0044] In step S2, the composite microcapsules are prepared by the following steps:
[0045] A1. 2.2 g of Danshen extract, 2.1 g of Astragalus extract, and 1.4 g of Pueraria extract were added to 30 g of ethanol and stirred at 700 r / min for 20 min. 3.5 g of tea polyphenols were added and stirred for 20 min. The mixture was stirred at 40 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0046] A2. 8 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 35°C for 30 min to obtain a β-cyclodextrin solution. 3.2 g of the composite material and 1 g of citric acid were added to 25 g of deionized water and stirred at 35°C for 30 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 40°C, ultrasonically treated at 40 kHz for 30 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0047] Example 2
[0048] A steaming and preparing process of Button Seven comprises the following steps:
[0049] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0050] S2. The puffed buttons seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 35 ℃ for 2.5h, steamed and dried to obtain steamed buttons seven herbs;
[0051] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 70°C, a screw speed of 210 r / min, a feed rate of 11 kg / h, and an amount of deionized water added of 50% of the weight of the washed buckle seven medicinal material;
[0052] In step S2, the mass ratio of the expanded buckle seven medicinal materials, composite microcapsules and water is 90:13:25;
[0053] In step S2, steaming is performed in a steamer at a temperature of 125° C. for 2 hours; and drying is performed at a temperature of 80° C. for 8 minutes.
[0054] In step S2, the composite microcapsules are prepared by the following steps:
[0055] A1. 2.3 g of Danshen extract, 2.2 g of Astragalus extract, and 1.5 g of Pueraria extract were added to 35 g of ethanol and stirred at 750 r / min for 25 min. 3.7 g of tea polyphenols were added and stirred for 25 min. The mixture was stirred at 45 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0056] A2. 10 g of β-cyclodextrin was added to 50 g of deionized water and stirred at 40°C for 35 minutes to obtain a β-cyclodextrin solution. 3.3 g of the composite material and 1.1 g of citric acid were added to 30 g of deionized water and stirred at 40°C for 35 minutes to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 50°C, ultrasonically treated at 45 kHz for 35 minutes, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 hours to obtain composite microcapsules.
[0057] Example 3
[0058] A steaming and preparing process of Button Seven comprises the following steps:
[0059] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0060] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ° C for 4h, steamed and dried to obtain a button seven herbs steamed product;
[0061] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0062] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0063] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0064] In step S2, the composite microcapsules are prepared by the following steps:
[0065] A1. 2.4 g of Danshen extract, 2.3 g of Astragalus extract, and 1.6 g of Pueraria extract were added to 40 g of ethanol and stirred at 800 r / min for 30 min. 3.9 g of tea polyphenols were added and stirred for 30 min. The mixture was stirred at 50 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0066] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material and 1.2 g of citric acid were added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0067] Comparative Example 1
[0068] A steaming and preparing process of Button Seven comprises the following steps:
[0069] S1. The button seven medicinal materials were cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips were removed. After washing with water to remove surface sediment, the materials were dried at 40 ° C for 10 min to obtain pre-treated button seven medicinal materials;
[0070] S2. The pretreated buttons seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ℃ for 4h, steamed and dried to obtain buttons seven herbs steamed products;
[0071] Wherein, in step S2, the mass ratio of the pre-treated button seven medicinal materials, composite microcapsules and water is 100:15:30;
[0072] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0073] In step S2, the composite microcapsules are prepared by the following steps:
[0074] A1. 2.4 g of Danshen extract, 2.3 g of Astragalus extract, and 1.6 g of Pueraria extract were added to 40 g of ethanol and stirred at 800 r / min for 30 min. 3.9 g of tea polyphenols were added and stirred for 30 min. The mixture was stirred at 50 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0075] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material and 1.2 g of citric acid were added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0076] Comparative Example 2
[0077] A steaming and preparing process of Button Seven comprises the following steps:
[0078] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0079] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ° C for 4h, steamed and dried to obtain a button seven herbs steamed product;
[0080] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0081] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0082] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0083] In step S2, the composite microcapsules are prepared by the following steps:
[0084] A1. 2.3 g of Astragalus extract and 1.6 g of Pueraria extract were added to 40 g of ethanol and stirred at 800 r / min for 30 min. 3.9 g of tea polyphenols were added and stirred for 30 min. The mixture was stirred at 50 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0085] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material and 1.2 g of citric acid were added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0086] Comparative Example 3
[0087] A steaming and preparing process of Button Seven comprises the following steps:
[0088] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0089] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ° C for 4h, steamed and dried to obtain a button seven herbs steamed product;
[0090] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0091] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0092] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0093] In step S2, the composite microcapsules are prepared by the following steps:
[0094] A1. 2.4 g of Danshen extract and 1.6 g of Pueraria extract were added to 40 g of ethanol and stirred at 800 r / min for 30 min. 3.9 g of tea polyphenols were added and stirred for 30 min. The mixture was stirred at 50 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0095] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material and 1.2 g of citric acid were added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0096] Comparative Example 4
[0097] A steaming and preparing process of Button Seven comprises the following steps:
[0098] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0099] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ° C for 4h, steamed and dried to obtain a button seven herbs steamed product;
[0100] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0101] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0102] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0103] In step S2, the composite microcapsules are prepared by the following steps:
[0104] A1. 2.4 g of Danshen extract and 2.3 g of Astragalus extract were added to 40 g of ethanol and stirred at 800 r / min for 30 min. 3.9 g of tea polyphenols were added and stirred for 30 min. The mixture was stirred at 50 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0105] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material and 1.2 g of citric acid were added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0106] Comparative Example 5
[0107] A steaming and preparing process of Button Seven comprises the following steps:
[0108] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0109] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ° C for 4h, steamed and dried to obtain a button seven herbs steamed product;
[0110] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0111] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0112] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0113] In step S2, the composite microcapsules are prepared by the following steps:
[0114] A1. 2.4g of Danshen extract, 2.3g of Astragalus extract, and 1.6g of Pueraria extract were mixed to obtain a composite material;
[0115] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material and 1.2 g of citric acid were added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0116] Comparative Example 6
[0117] A steaming and preparing process of Button Seven comprises the following steps:
[0118] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0119] S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly, and then simmered at 40 ° C for 4h, steamed and dried to obtain a button seven herbs steamed product;
[0120] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0121] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0122] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0123] In step S2, the composite microcapsules are prepared by the following steps:
[0124] A1. 2.4 g of Danshen extract, 2.3 g of Astragalus extract, and 1.6 g of Pueraria extract were added to 40 g of ethanol and stirred at 800 r / min for 30 min. 3.9 g of tea polyphenols were added and stirred for 30 min. The mixture was stirred at 50 ° C until the solvent evaporated and dried at 20 ° C for 24 h to obtain a composite material.
[0125] A2. 12 g of β-cyclodextrin was added to 45 g of deionized water and stirred at 45°C for 40 min to obtain a β-cyclodextrin solution. 3.4 g of the composite material was added to 35 g of deionized water and stirred at 45°C for 40 min to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and stirred, then heated to 60°C, ultrasonically treated at 50 kHz for 40 min, cooled to room temperature, and the solid was filtered out. The solid was washed three times with deionized water and frozen at -20°C for 24 h to obtain composite microcapsules.
[0126] Comparative Example 7
[0127] A steaming and preparing process of Button Seven comprises the following steps:
[0128] S1. The button seven medicinal materials are cleaned and non-medicinal residual stems, fibrous roots, dead leaves, stones, and metal chips are removed. After washing to remove surface sediment, the button seven medicinal materials are puffed and dried at 40 ° C for 10 min to obtain puffed button seven medicinal materials;
[0129] S2. The puffed buttons seven herbs, composite materials and water were mixed and stirred evenly, and then simmered at 40 ℃ for 4h, steamed and dried to obtain steamed buttons seven herbs;
[0130] In step S1, the puffing process is carried out using a twin-screw extruder, with a barrel temperature of 80°C, a screw speed of 220 r / min, a feed rate of 12 kg / h, and an amount of deionized water added of 55% of the weight of the washed buckle seven medicinal material;
[0131] In step S2, the mass ratio of the expanded Kouziqi medicinal material, the composite microcapsules and water is 100:15:30;
[0132] In step S2, steaming is performed in a steamer at a temperature of 130° C. for 4 hours, and drying is performed at a temperature of 90° C. for 10 minutes.
[0133] In step S2, the composite material is specifically prepared by the following steps:
[0134] 2.4 g of Salvia miltiorrhiza extract, 2.3 g of Astragalus membranaceus extract, and 1.6 g of Pueraria lobata extract were added to 40 g of ethanol, stirred at 800 r / min for 30 min, 3.9 g of tea polyphenols were added, and stirring was continued for 30 min. The mixture was stirred at 50°C until the solvent evaporated, and dried at 20°C for 24 h to obtain a composite material.
[0135] The performance of the steamed products of Kouziqi medicinal materials prepared in Examples 1-3 and Comparative Examples 1-7 was tested.
[0136] Use a rotary medicine cutting machine to cut the steamed Panax notoginseng into thin slices with a thickness of 2 mm. Observe the state of the Panax notoginseng slices under a microscope to determine whether the starch in the steamed Panax notoginseng is gelatinized. Observe whether the color of the cut surface is brown to determine the stability of the steamed Panax notoginseng.
[0137] Determination of the yield of steamed products of Kouziqi medicinal materials: record the weight of the puffed Kouziqi medicinal materials and the composite material (m1), and the steamed products of Kouziqi medicinal materials (m2). The yield = m2 / m1×100%.
[0138] Take 1g of steamed Kouziqi medicinal material and add it to 50mL of ethanol. Ultrasonic extraction was performed at 40kHz for 40min. The mixture was cooled and weighed. The weight loss was supplemented with ethanol. The mixture was centrifuged at 13000r / min for 10min. The supernatant was collected as the test sample.
[0139] Content determination: The samples were determined by high performance liquid chromatography to determine the total content of saponins (saponins include ginsenoside Ro, pseudoginsenoside RT1, bamboo ginsenoside IV, bamboo ginsenoside IVa, ginger-like notoginseng glycoside R1, pseudoginsenoside RP1, and ginsenoside RK1). The total content of saponins in the unprocessed Radix Notoginseng was 5.6%, which was used as the control group.
[0140] High performance liquid chromatography: Waters CORTECS UPLC T3 column (2.1×100 mm, 1.6 μm), mobile phase: 0.1% phosphoric acid (A); acetonitrile (B), gradient elution (0-7 min, 30-32% B; 7-10 min, 32-35% B; 10-12 min, 35-43% B; 12-14 min, 43-43% B; 14-16 min, 43-47% B; 16-21 min, 47-54% B; 21-22 min, 54-54% B; 22-23 min, 54-56% B; 23-24 min, 56-56% B; 24-30 min, 56-70% B; 30-35 min, 70-30% B); flow rate 0.3 mL / min; detection wavelength 203 nm; column temperature 30°C; injection volume 1 μL.
[0141] The test results are shown in Table 1 below.
[0142] Table 1 Performance test of steamed products of Kouziqi medicinal materials prepared in Examples 1-3 and Comparative Examples 1-7
[0143]
[0144]
[0145] It can be seen from the data in Table 1 that the steamed products of Kouziqi medicinal materials prepared in Examples 1-3 have high saponin content and good stability.
[0146] In comparative example 1, the steamed product of the button seven medicinal material prepared without puffing the button seven medicinal material in step S1 has a decreased saponin content and poor stability, which proves that the button seven medicinal material is puffed to make the surface of the button seven medicinal material have a porous structure, which is conducive to the release of saponin components in the button seven medicinal material. Its porous structure increases the contact area with the composite microcapsule, making it easier for the composite microcapsule to enter the interior of the button seven medicinal material, thereby improving the processing efficiency, increasing the rare saponin content, reducing starch gelatinization and improving the stability of the steamed product of the button seven medicinal material.
[0147] In comparative example 2, the composite microcapsules prepared without adding Salvia miltiorrhiza extract were mixed with the medicinal materials of Kouziqi and processed. The saponin content of the prepared steamed product of Kouziqi medicinal materials decreased, proving that the fat-soluble components in Salvia miltiorrhiza extract can promote the dissolution and retention of saponin components of Kouziqi, reduce the loss of components during the processing process, and increase the content of rare saponins.
[0148] In comparative example 3, the composite microcapsules prepared without adding astragalus extract were mixed with the medicinal materials of button seven for processing. The saponin content of the prepared steamed product of button seven medicinal materials decreased, which proved the surface activity of astragalus extract, which can optimize the wetting effect during the processing of button seven medicinal materials, increase the moisturizing effect of button seven medicinal materials, and is beneficial to increase the content of rare saponins in the steamed product of button seven medicinal materials.
[0149] In comparative example 4, the composite microcapsules prepared without adding Pueraria lobata extract were mixed with the medicinal materials of Kouziqi for preparation. The prepared steamed product of Kouziqi medicinal materials had a decreased saponin content and poor stability, which proved that Pueraria lobata extract can inhibit the oxidative degradation of saponin components, improve the stability of the steamed product of Kouziqi medicinal materials, and avoid the loss of components during the preparation process.
[0150] In comparative example 5, the composite microcapsules prepared without adding tea polyphenols were mixed with the Chinese herbal medicine Kuziqi for preparation. The steamed product of the prepared Kuziqi medicinal material had a decreased saponin content and poor stability, which proved that the phenolic hydroxyl groups contained in tea polyphenols could form a stable system by hydrogen bonding and cross-linking with the active groups in Salvia miltiorrhiza extract, Astragalus membranaceus extract, and Pueraria lobata extract, which was beneficial for the encapsulation of β-cyclodextrin. In addition, tea polyphenols have an antioxidant effect, can significantly scavenge DPPH free radicals, prevent the thermal decomposition of saponins, and have a synergistic effect with Pueraria lobata extract in inhibiting the oxidative degradation of saponin components.
[0151] In comparative example 6, the composite microcapsules prepared without adding citric acid were mixed with the medicinal materials of Kouziqi for preparation. The steamed product of Kouziqi medicinal materials prepared had a decreased saponin content and poor stability, which proved that citric acid, as a cross-linking agent, can combine with tea polyphenols and β-cyclodextrin through chemical bonds, thereby increasing the cross-linking density of the microcapsules and thus increasing the embedding rate. In addition, citric acid can inhibit starch gelatinization, prevent starch from gelatinizing easily, wrap the saponin components in Kouziqi, form a physical barrier, and reduce the dissolution rate of saponins.
[0152] In Comparative Example 7, the composite microcapsules were replaced with composite materials and mixed with the button seven medicinal materials for preparation. The saponin content of the prepared button seven medicinal material steamed product decreased and the stability was poor, which proved that using the composite material as the core material and β-cyclodextrin and citric acid as the capsule material to form microcapsules can improve the stability of the composite material. During the preparation process of the puffed button seven medicinal materials, the internal moisture evaporated rapidly, the outer layer dried and shrank, and stress was generated, which caused the composite microcapsules entering the puffed button seven medicinal materials to rupture, releasing the composite material, which played a role inside the button seven medicinal materials and improved the preparation efficiency.
[0153] Throughout the specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
[0154] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the described specific embodiments or replace them in similar ways. As long as they do not deviate from the invention or exceed the scope defined by the claims, they should all fall within the scope of protection of the present invention.
Claims
1. A steaming process for preparing button seven, characterized in that: The following steps are involved: S1. After removing impurities and cleaning the buttons, the buttons are puffed and dried to obtain puffed buttons. S2. The puffed button seven herbs, composite microcapsules and water were mixed and stirred evenly. After 1-4h of incubation at 30-40 ℃, the mixture was steamed and dried to obtain steamed buttons seven herbs. The composite microcapsule is made of a composite material as a core material and β-cyclodextrin and citric acid as capsule materials; The composite material is obtained by mixing salvia miltiorrhiza extract, astragalus root extract, kudzu root extract and tea polyphenols.
2. The steaming process of Button 7 according to claim 1 is characterized in that: In step S1, the puffing treatment adopts a twin-screw extruder, the barrel temperature is 60-80°C, the screw speed is 200-220r / min, the feed rate is 10-12kg / h, and the amount of deionized water added is 45-55% of the weight of the cleaned buckle seven medicinal material.
3. The steaming process of Button 7 according to claim 1 is characterized in that: In step S2, the mass ratio of the expanded Kouziqi medicinal material, composite microcapsules and water is (80-100):(10-15):(20-30).
4. The steaming process of Button 7 according to claim 1 is characterized in that: In step S2, the steaming temperature is 115-130° C., and the steaming time is 1-4 hours; the drying temperature is 60-90° C., and the drying time is 5-10 minutes.
5. The steaming process of Button 7 according to claim 1 is characterized in that: In step S2, the composite microcapsules are prepared by the following steps: A1. Salvia miltiorrhiza extract, Astragalus extract, and Pueraria root extract were added to ethanol and stirred at 700-800 r / min for 20-30 min. Tea polyphenols were added and stirred for 20-30 min. The mixture was stirred at 40-50 ° C until the solvent evaporated and dried to obtain a composite material. A2. β-cyclodextrin was added to deionized water and stirred at 35-45°C for 30-40 minutes to obtain a β-cyclodextrin solution. The composite material and citric acid were added to deionized water and stirred at 35-45°C for 30-40 minutes to obtain a composite solution. The composite solution and the β-cyclodextrin solution were mixed and ultrasonically treated. The mixture was cooled to room temperature, and the solid was filtered, washed, and freeze-dried to obtain composite microcapsules.
6. The steaming process of Button 7 according to claim 5 is characterized in that: In step A1, the mass ratio of the salvia miltiorrhiza extract, astragalus root extract, kudzu root extract, ethanol, and tea polyphenols is (2.2-2.4):(2.1-2.3):(1.4-1.6):(30-40):(3.5-3.9).
7. The steaming process of Button 7 according to claim 5 is characterized in that: In step A2, the mass ratio of β-cyclodextrin to deionized water is (8-12):(45-55).
8. The steaming process of Button 7 according to claim 5 is characterized in that: In step A2, the mass ratio of the composite material, citric acid and deionized water is (3.2-3.4):(1-1.2):(25-35).
9. The steaming process of Button 7 according to claim 5 is characterized in that: In step A2, the ultrasonic treatment temperature is 40-60° C., the ultrasonic treatment frequency is 40-50 KHz, and the ultrasonic treatment time is 30-40 min.