Actinomycete antibacterial agent and application thereof in prevention and control of common diseases of Chinese fir

Abstract

The invention relates to the technical field of disease prevention and control, in particular to an actinomycete antibacterial agent and application of the actinomycete antibacterial agent in prevention and control of common diseases of China fir. The antibacterial agent is fermentation liquor of actinomycetes FY4; the preparation process of the fermentation liquor comprises the following steps: inoculating actinomycetes FY4 on an ISP2 agar culture medium by a Z-shaped streaking method, culturing in a 28 DEG C constant temperature box, then taking out a fungus cake by a sterile puncher, transferring the fungus cake into a soybean powder immersion liquid culture medium, and additionally adding composite trace elements into the culture medium; meanwhile, intermittent ultrasonic treatment is carried out in the culture process; placing the culture medium in a shaking table for continuous culture; after fermentation is finished, centrifugally separating supernate, and finally filtering by using a water system filter membrane to obtain fermentation filtrate; the anti-microbial agent based on the actinomycetes FY4 zymophyte liquid provided by the invention has a relatively strong antibacterial effect in the field of prevention and control of Chinese fir diseases, and the actinomycetes FY4 zymophyte liquid shows outstanding antibacterial activity.

Description

Technical Field

[0001] The present invention relates to the technical field of disease prevention and control, in particular to an actinomycete antifungal agent and application thereof in preventing and controlling common diseases of Chinese fir. Background Art

[0002] Chinese fir is an important fast-growing timber species in southern my country and occupies a key position in the forestry economy. Due to its rapid growth and excellent quality, it is widely used in construction, furniture manufacturing and other fields. However, Chinese fir faces severe disease threats during its growth process, which seriously restricts its healthy growth and the improvement of forestry production efficiency.

[0003] Actinomycetes are a class of Gram-positive bacteria with significant application value, named for their filamentous, branching structure. They are widely distributed in soil, water, and on the surfaces of plants and animals, producing a rich variety of metabolites, including antibiotics, enzymes, and vitamins. In the field of biological control, actinomycetes are gaining further application due to their unique antibacterial properties and environmental friendliness.

[0004] Chinese fir is threatened by a variety of pathogens, including those from the genera Alternaria, Fusarium, Colletotrichum, and Phytophthora. These pathogens cause common diseases such as leaf blight, red blight, and anthracnose. Leaf blight causes numerous lesions on fir leaves, which gradually turn yellow and fall off, severely impacting photosynthesis. Red blight rapidly withers young shoots and leaves, reducing plant vigor. Anthracnose forms black lesions on the stems, branches, and leaves, causing tissue necrosis and even the death of the entire plant. Once these diseases break out, they not only slow growth and reduce wood quality, but also cause widespread tree mortality, resulting in significant economic losses to forestry production.

[0005] Currently, the use of chemical pesticides is still widespread in the prevention and control of Chinese fir diseases. While they can effectively control the spread of diseases in the short term, their long-term, extensive use presents numerous serious problems. For one thing, chemical pesticide residues pollute the soil, water, and air, disrupting ecological balance and affecting biodiversity. Furthermore, pathogens can easily develop resistance to chemical pesticides, leading to increasing pesticide dosages and a gradual decline in control effectiveness. Furthermore, chemical pesticides can enter the human body through the food chain, posing a potential threat to human health.

[0006] Therefore, developing green, efficient and sustainable biological control technologies to replace or reduce the use of chemical pesticides is of great significance for ensuring the healthy growth of Chinese fir, maintaining ecological and environmental safety and promoting the sustainable development of forestry. Summary of the Invention

[0007] In view of the problems existing in the prior art, the present invention provides an actinomycete antifungal agent and its application in preventing and controlling common diseases of Chinese fir.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] An actinomycete antifungal agent, wherein the active ingredient of the antifungal agent is derived from the unique actinomycete FY4;

[0010] The antibacterial agent is the fermentation broth of actinomycetes FY4;

[0011] The fermentation broth preparation process includes: inoculating actinomycetes FY4 on ISP2 agar medium using a Z-streaking method, placing the culture in a 28°C constant temperature incubator for 5-6 days, then taking out 10 bacterial cakes with a diameter of 8 mm using a sterile punch, transferring the culture cakes to 100 mL of soybean powder infusion medium, and additionally adding complex trace elements to the culture medium, wherein the complex trace elements are zinc, iron, and manganese ions; simultaneously, performing intermittent ultrasonic treatment during the culturing process, ultrasonic treatment for 10-15 minutes every 2-3 hours, so as to improve the bacterial body's absorption efficiency of nutrients and secretion of metabolites; placing the culture in a shaker with a shaking rate of 150-180 rpm and a temperature of 28°C, and continuously culturing for 10-12 days; after the fermentation is completed, separating the supernatant by centrifugation at 5000-5500 rpm and 20°C for 20-24 minutes, repeating the centrifugation operation once, and finally filtering the culture filtrate using a 0.22 μm water filter membrane to obtain a fermentation filtrate.

[0012] As a further technical solution, the ultrasonic power is controlled at 100-120W.

[0013] As a further technical solution, the basic component ratio of the soybean powder infusion culture medium is: 20-30g soybean powder, 10-20g glucose, 0.5-1.5g sodium chloride, 0.5-1.0g potassium dihydrogen phosphate, and 0.2-0.5g magnesium sulfate, which are dissolved in 1000mL distilled water and the pH is adjusted to 6-7.

[0014] As a further technical solution, the addition amount of the composite trace elements is 0.8-1.2 mg of zinc ions, 1-1.5 mg of iron ions, and 1.5-1.8 mg of manganese ions per liter of soybean powder infusion culture medium to promote the growth and metabolism of actinomycetes.

[0015] As a further technical solution, the antibacterial agent has a significant inhibitory effect on Chinese fir pathogens;

[0016] The Chinese fir pathogens include Alternaria alternata, Alternaria fusca, Colletotrichum gloeosporioides, and Colletotrichum gloeosporioides. Experiments show that the antibacterial agent has an inhibition rate of more than 95% on the mycelial growth of these pathogens, and also has varying degrees of inhibition on their spore germination, effectively reducing the risk of pathogenic bacteria invading Chinese fir and ensuring the healthy growth of Chinese fir.

[0017] As a further technical solution, the antibacterial agent has good stability under various environmental conditions; within the pH range of pH 4-10, the inhibition rate of mycelium growth of the perennial Heterobasidiomycete Ha03010 is always maintained at 100%; within the temperature range of 4°C-100°C, the inhibition of Ha03010 mycelium maintains biological activity; under ultraviolet light conditions of 1-12 hours, the inhibition rate of Ha03010 mycelium growth is stable at 100%; during storage at room temperature for 1-72 hours, the inhibition rate of Ha03010 mycelium growth is still 100%. This stability ensures the effectiveness of the antibacterial agent in different environments and extends the shelf life and scope of application of the product.

[0018] Application of actinomycete fungicide in preventing and controlling common diseases of Chinese fir.

[0019] As a further technical solution, the common fir diseases include fir leaf blight, fir red blight, and fir anthracnose. The actinomycete fungicide prevents and controls these diseases by inhibiting the growth of the pathogens that cause them. Furthermore, the agent can be used in conjunction with beneficial insects or other beneficial microorganisms used in biological control to establish a mutually beneficial, symbiotic ecological control system, further improving the control efficiency of common fir diseases, reducing the use of chemical pesticides, and protecting the ecological environment.

[0020] As a further technical solution, the application method not only comprises mixing the actinomycete antifungal agent with a PDA medium melted to about 50° C. at a concentration of 50% (v / v) to prepare a poisoned PDA medium to inhibit the growth of mycelium of Chinese fir pathogens, but also can adopt various application methods such as spraying and root irrigation. During the spray application, a surfactant is added to enhance the adhesion and permeability of the antifungal agent on the surface of Chinese fir leaves, thereby improving the antifungal effect.

[0021] The surfactant is sodium dodecylbenzenesulfonate, which is added in an amount of 1-2 wt%.

[0022] As a further technical solution, the application also includes using the actinomycete fungicide to treat fir seeds, thereby increasing the germination rate of fir seeds and the length of radicles, and promoting the growth of fir seedlings;

[0023] After treating Chinese fir seeds with 10-12% actinomycete fungicide for 60-75 minutes, the seed germination rate can reach more than 90%.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The antibacterial agent based on the fermentation liquid of actinomycetes FY4 provided by the present invention has a strong antibacterial effect in the field of fir disease prevention and control.

[0026] First, the fermentation liquid of the actinomycete FY4 of the present invention exhibits outstanding antibacterial activity, primarily due to the production of certain volatile substances with antibacterial properties by the actinomycete FY4. The fermentation liquid, prepared using the specific process of the present invention, exhibits strong inhibitory activity against Chinese fir pathogens, such as Alternaria, Fusarium, Colletotrichum, and Phytophthora. The inhibition rate for mycelial growth of these pathogens exceeds 95%, and spore germination is also significantly inhibited. This excellent antibacterial effect effectively reduces the risk of pathogenic bacteria infecting Chinese fir, ensuring its healthy growth, thereby increasing timber yield and quality and significantly enhancing forestry production efficiency.

[0027] The present invention has unique advantages over full ultrasonic treatment in the preparation process. Although full ultrasonic treatment can also have a certain effect on the bacteria, high-intensity and long-term ultrasound will cause excessive damage to the actinomycete cells, destroy the integrity of the cell structure, cause a large amount of leakage of intracellular substances, and affect the normal metabolism of the cells and the synthesis and secretion of active ingredients. Intermittent ultrasonic treatment, while ensuring the increase in cell membrane permeability, gives the cells enough recovery time. This can not only promote nutrients to enter the cells more efficiently and meet the growth and metabolic needs of the bacteria, but also avoid cell death due to excessive damage, so that actinomycetes can continuously and stably synthesize and secrete antibacterial active ingredients. At the same time, intermittent ultrasonic treatment can also stimulate the stress response of actinomycetes, inducing them to produce more metabolites with antibacterial activity, thereby improving the overall antibacterial effect of the fermentation liquid.

[0028] The addition of complex trace elements (zinc, iron, and manganese ions) plays a key role in the fermentation process. Zinc ions are a component or activator of many enzymes and participate in various metabolic reactions within cells. They can enhance the activity of antioxidant enzymes within actinomycete cells, improve the cells' antioxidant capacity, reduce damage to cells caused by oxidative stress, ensure normal physiological functions of cells, and provide a stable metabolic environment for the synthesis of active ingredients. Iron ions are an indispensable element in cellular respiration and electron transfer. They participate in redox reactions within cells, provide energy for the growth and metabolism of actinomycetes, and are also related to the synthesis of active substances such as certain antibiotics, and can promote the biosynthesis of antibacterial active ingredients. Manganese ions participate in important physiological processes such as photosynthesis and nitrogen metabolism within actinomycete cells, regulate intracellular ion balance, affect the structure and function of cell walls and cell membranes, help maintain cell integrity and stability, and thus improve the efficiency of actinomycete synthesis and secretion of active ingredients. Complex trace elements and the growth and metabolic processes of actinomycetes work together to promote the improvement of the antibacterial activity of the fermentation broth.

[0029] Furthermore, the antibacterial agent of the present invention exhibits excellent stability under a variety of environmental conditions. Under conditions such as pH 4-10, 4°C-100°C, UV irradiation for 1-12 hours, and room temperature storage for 1-72 hours, the antibacterial agent consistently maintains a 100% inhibition rate against the mycelial growth of the perennial Heterobasidiomycete Ha03010. This excellent stability enables it to adapt to diverse environmental conditions in practical applications, ensuring the sustainability and reliability of its antibacterial effect. Furthermore, as a biological agent, the antibacterial agent is environmentally friendly, effectively reducing the use of chemical pesticides, reducing environmental pollution, and protecting ecological balance. This aligns with the concept of green and sustainable development, offering broad application prospects and significant social benefits.

[0030] Figures in the specification

[0031] Figure 1 This is a graph showing the inhibitory effect of actinomycete FY4 on 8 Chinese fir pathogens. DETAILED DESCRIPTION

[0032] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0033] Preparation and Application of Actinomycete Antimicrobial Agent

[0034] The invention provides an actinomycete antifungal agent, the active ingredient of which is derived from actinomycete FY4, and the antifungal agent is a fermentation liquid of actinomycete FY4.

[0035] The actinomycete FY4 used in the present invention was isolated from the root tip mycorrhizae of 6-month-old densely planted matsutake seedlings at a specific matsutake production base in Wakayama Prefecture, Japan. The PCR amplification fragments of its 16SrRNA, atpD, rpoB, and trpB genes showed specific lengths of 1420 bp, 706 bp, 735 bp, and 726 bp, respectively. As shown in Table 1, the primers and DNA sequences used for PCR amplification correspond to the accession numbers PP434658, PP230168, PP230166, and PP230167 in GenBank. These genetic characteristics can be used to accurately identify this strain and distinguish it from other actinomycetes.

[0036] The actinomycete FY4 (Streptomyces blastmyceticus FY4) used in the present invention has been deposited in the China Culture Collection on March 24, 2025, with the deposit number CCTCCNO2: M2025485;

[0037] Fermentation broth preparation process

[0038] Inoculation and culture: Inoculate actinomycetes FY4 onto ISP2 agar medium using the Z-streak method and culture in a 28°C incubator for 5-6 days to ensure full activation and reproduction of the bacteria.

[0039] Transfer and addition of trace elements: Use a sterile punch to take 10 bacterial cakes with a diameter of 8 mm and transfer them to 100 mL of soybean powder infusion culture medium. Add zinc, iron, and manganese ion complex trace elements to optimize the metabolic environment of actinomycetes.

[0040] Culture medium ingredients: Soybean powder infusion culture medium is prepared by dissolving 20-30g soybean powder, 10-20g glucose, 0.5-1.5g sodium chloride, 0.5-1.0g potassium dihydrogen phosphate, and 0.2-0.5g magnesium sulfate in 1000mL distilled water and adjusting the pH to 6-7 to provide suitable nutritional conditions for the growth of actinomycetes.

[0041] Trace element addition amount: Add 0.8-1.2mg zinc ion, 1-1.5mg iron ion, and 1.5-1.8mg manganese ion per liter of culture medium to precisely control the nutritional composition.

[0042] Ultrasonic treatment and shaking culture: Intermittent ultrasonic treatment is performed during the culture process, with an ultrasonic power of 100-120W and 10-15 minutes every 2-3 hours to enhance cell membrane permeability; the culture is placed on a shaker at a speed of 150-180 rpm and a temperature of 28°C for 10-12 days to ensure sufficient growth of the bacteria.

[0043] Separation and filtration: After the fermentation is completed, the supernatant is separated by centrifugation at 5000-5500rpm and 20℃ for 20-24min. After repeated centrifugation, it is filtered with a 0.22μm water filter membrane to obtain pure fermentation filtrate, which is the antibacterial agent.

[0044] Performance characteristics of antibacterial agents

[0045] Inhibitory effect on Chinese fir pathogens: The inhibition rate on the mycelial growth of Chinese fir pathogens such as Alternaria alternata, Alternaria fusca, Colletotrichum gloeosporioides, etc. is over 95%, which significantly inhibits spore germination and reduces the risk of Chinese fir being attacked.

[0046] Environmental stability: Under the conditions of pH 4-10, 4℃-100℃, ultraviolet light exposure for 1-12h and storage at room temperature for 1-72h, the mycelial growth inhibition rate of the perennial Heterobasidiomycete Ha03010 is always maintained at 100%, with good stability.

[0047] Application of antifungal agents in preventing and controlling common diseases of Chinese fir

[0048] Types of diseases prevented and controlled: Suitable for common diseases such as fir leaf blight, fir red blight and fir anthracnose.

[0049] Application method

[0050] Mix with PDA culture medium: Mix with PDA culture medium melted at about 50°C at a rate of 50% (v / v) to make a poisonous PDA culture medium to inhibit the growth of pathogenic bacteria hyphae.

[0051] Spray: Add 1-2wt% sodium dodecylbenzene sulfonate as a surfactant and then spray to enhance the adhesion effect.

[0052] Root irrigation: Direct irrigation to the roots, affecting the root system and soil environment.

[0053] Treatment of Chinese fir seeds: Treat the seeds with 10-12% concentration of antifungal agent for 60-75 minutes, and the seed germination rate can reach more than 90%.

[0054] The following are specific embodiments:

[0055] Example 1

[0056] Prepare soybean powder infusion culture medium: weigh 25 g soybean powder, 15 g glucose, 1 g sodium chloride, 0.8 g potassium dihydrogen phosphate, and 0.3 g magnesium sulfate, dissolve in 1000 mL distilled water, and adjust the pH to 6.5.

[0057] Add trace elements: add 1 mg zinc ion, 1.2 mg iron ion, and 1.6 mg manganese ion per liter of culture medium.

[0058] The inoculation, ultrasonic treatment, shaking culture, centrifugation and filtration were completed according to the standard process to prepare the actinomycete antibacterial agent.

[0059] Example 2

[0060] Culture medium preparation: Weigh 22 g soybean powder, 12 g glucose, 0.8 g sodium chloride, 0.6 g potassium dihydrogen phosphate, and 0.25 g magnesium sulfate, dissolve in 1000 mL distilled water, and adjust the pH to 6.2.

[0061] Trace element addition: add 0.9 mg zinc ion, 1.1 mg iron ion, and 1.5 mg manganese ion per liter of culture medium.

[0062] The remaining operations were the same as in Example 1 to obtain an actinomycete antifungal agent.

[0063] Example 3

[0064] Preparation of culture medium: Weigh 28 g of soybean powder, 18 g of glucose, 1.2 g of sodium chloride, 0.9 g of potassium dihydrogen phosphate, and 0.4 g of magnesium sulfate, dissolve in 1000 mL of distilled water, and adjust the pH to 6.8.

[0065] Trace element addition: Add 1.1 mg zinc ion, 1.3 mg iron ion, and 1.7 mg manganese ion per liter of culture medium.

[0066] The actinomycete antifungal agent was prepared according to the standard operating procedures.

[0067] Comparative Example 1

[0068] The preparation process was basically the same as that in Example 1, except that intermittent ultrasonic treatment was not performed during the culture process, but ultrasonic treatment was performed throughout the process. The remaining steps, such as culture medium preparation, trace element addition, inoculation, shaking culture conditions, centrifugation and filtration, were consistent with those in Example 1 to prepare a comparative antibacterial agent.

[0069] Comparative Example 2

[0070] During the preparation process, the culture medium contained only basic components, and no zinc, iron, and manganese ion complex trace elements were added. The remaining operating steps, including inoculation, ultrasonic treatment, shaking culture, centrifugation and filtration, were performed with reference to Example 1 to obtain a comparative antibacterial agent.

[0071] Table 1 Primers and DNA sequences used for PCR amplification

[0072]

[0073]

[0074] test

[0075] Field application effect test

[0076] Test Method: Multiple test plots were divided in a fir forest, each planted with identical fir seedlings. The antimicrobial agents of Examples 1-3 were sprayed at 10% concentration at 15-day intervals. A blank control plot was sprayed with water, and a control plot was sprayed with the comparative antimicrobial agent. The incidence of fir leaf blight, red blight, and anthracnose was recorded throughout the growing season.

[0077] Table 2

[0078]

[0079] It can be seen from Table 2 that the antibacterial agent of the examples significantly reduced the incidence of common diseases of Chinese fir; the antibacterial agent of the comparative example had a weaker control effect, and the disease in the blank control plot was serious.

[0080] The fermentation broth in Example 1 was analyzed for active substances in the fermentation broth using liquid chromatography-mass spectrometry / mass spectrometry (LC-MS / MS) from Shanghai Applied Protein Technology Co., Ltd. (Shanghai). The results are shown in Table 3:

[0081] Table 3

[0082]

[0083]

[0084] As shown in Table 3 , 915 compounds were identified in the FY4 fermentation broth after database matching, which were divided into 13 superclasses, among which the most abundant were organic acids and their derivatives (23.5%), lipids and lipid molecules (21.7%), and benzene (10.4%).

[0085] The actinomycetes FY4 of Example 1 was matched with the Chinese fir pathogens and cultured for 10 days. It was found that the actinomycetes FY4 showed different degrees of antibacterial effect on the Chinese fir pathogens. Figure 1 ,This experiment shows that actinomycetes FY4 can produce some volatile substances with ,antibacterial effects. According to Table 4, ,the antibacterial effects are SMCG, HN, XXG, SDHG, MQ, CQ, SJ, and NC, ,from strong to weak;

[0086] Chinese fir leaf blight Alternaria Shandongensi (SDHG), Fusarium fujikuroi (HN);

[0087] Pseudopestalotiopsis Chinensis (NC)

[0088] Colletotrichum gloeosporioides (SMCG),

[0089] Fruit anthrax Colletotrichum fructicola (MQ);

[0090] Table 4

[0091]

[0092]

[0093] Note: The data listed in the table are the average values ​​of 3 groups of repetitions. Data without the same lowercase letters indicate significant differences (P < 0.05).

[0094] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the content of this specification.

Claims

1. An actinomycete antifungal agent, characterized in that: The active ingredient of the antibacterial agent is derived from Actinomycetes FY4; The antibacterial agent is the fermentation broth of actinomycetes FY4; The fermentation broth preparation process includes: inoculating actinomycetes FY4 on ISP2 agar medium using a Z-streaking method, placing the culture in a 28°C constant temperature incubator for 5-6 days, then taking out 10 bacterial cakes with a diameter of 8 mm using a sterile punch, transferring the culture cakes to 100 mL of soybean powder infusion medium, and additionally adding complex trace elements to the culture medium, wherein the complex trace elements are zinc, iron, and manganese ions; simultaneously, performing intermittent ultrasonic treatment during the culturing process, ultrasonic treatment for 10-15 minutes every 2-3 hours; placing the culture medium on a shaker with a shaking rate of 150-180 rpm and a temperature of 28°C, and continuously culturing for 10-12 days; after the fermentation is completed, separating the supernatant by centrifugation at 5000-5500 rpm and 20°C for 20-24 minutes, repeating the centrifugation operation once, and finally filtering the culture medium using a 0.22 μm water filter membrane to obtain a fermentation filtrate.

2. The actinomycete antifungal agent according to claim 1, characterized in that The ultrasonic power is controlled at 100-120W.

3. The actinomycete antifungal agent according to claim 1, characterized in that The basic component ratio of the soybean powder infusion culture medium is: 20-30g soybean powder, 10-20g glucose, 0.5-1.5g sodium chloride, 0.5-1.0g potassium dihydrogen phosphate, and 0.2-0.5g magnesium sulfate, which are dissolved in 1000mL distilled water and the pH is adjusted to 6-7.

4. The actinomycete antifungal agent according to claim 3, characterized in that The addition amount of the composite trace elements is 0.8-1.2 mg of zinc ion, 1-1.5 mg of iron ion, and 1.5-1.8 mg of manganese ion per liter of soybean powder infusion culture medium.

5. The actinomycete antifungal agent according to claim 1, characterized in that The antibacterial agent has a significant inhibitory effect on Chinese fir pathogens; The Chinese fir pathogens include Alternaria alternata, Alternaria fusca, Colletotrichum gloeosporioides, and Colletotrichum gloeosporioides. Experiments show that the antibacterial agent has an inhibition rate of more than 95% on the mycelial growth of these pathogens, and also has varying degrees of inhibition on their spore germination, effectively reducing the risk of pathogenic bacteria invading Chinese fir and ensuring the healthy growth of Chinese fir.

6. The actinomycete antifungal agent according to claim 1, characterized in that The antibacterial agent has good stability under various environmental conditions. Within the pH range of 4-10, the mycelial growth inhibition rate of the perennial Heterobasidiomycete Ha03010 is always maintained at 100%; within the temperature range of 4°C-100°C, the mycelial inhibition of Ha03010 maintains biological activity; under ultraviolet irradiation for 1-12 hours, the mycelial growth inhibition rate of Ha03010 is stable at 100%; and during storage at room temperature for 1-72 hours, the mycelial growth inhibition rate of Ha03010 remains at 100%.

7. Use of the actinomycete antifungal agent according to any one of claims 1 to 6 in preventing and controlling common diseases of Chinese fir.

8. The use according to claim 7, characterized in that The common diseases of Chinese fir include Chinese fir leaf blight, Chinese fir red blight and Chinese fir anthracnose.

9. The use according to claim 7, characterized in that In addition to mixing the actinomycete antifungal agent with a PDA culture medium melted to about 50° C. at a concentration of 50% (v / v) to prepare a poisoned PDA culture medium to inhibit the growth of Chinese fir pathogen mycelium, the application method can also adopt various application methods such as spraying and root irrigation; in the spray application, a surfactant is also added; the surfactant is sodium dodecylbenzene sulfonate, which is added in an amount of 1-2wt%.

10. The use according to claim 6, characterized in that The application also includes using the actinomycete fungicide to treat fir seeds; After treating Chinese fir seeds with 10-12% actinomycete fungicide for 60-75 minutes, the seed germination rate can reach more than 90%.

Images