High-activity lumbrukinase extraction equipment and method

Abstract

The invention discloses high-activity lumbrukinase extraction equipment and method. The equipment comprises an equipment bracket, a cleaning assembly, a grinding assembly and a separation assembly, the cleaning assembly comprises a first transverse plate, a cleaning outer cylinder arranged on the first transverse plate and an inner net cylinder arranged in the cleaning outer cylinder in a sleeved mode. A gland is arranged at the top end of the outer cleaning cylinder and internally provided with a cleaning coil pipe. The grinding assembly comprises a second transverse plate, a grinding seat arranged on the second transverse plate, a rotating seat clamped in the grinding seat and a grinding motor for providing power for the grinding seat; the separation assembly comprises a separation barrel, a conical separation mesh enclosure and a material stirring fan blade which are arranged in the separation barrel, and a material stirring motor for providing power for the material stirring fan blade; the earthworm adults are cleaned, ground and separated, so that the lumbrukinase in the earthworm bodies is completely separated, and the purity and activity of the lumbrukinase are effectively improved; meanwhile, due to the integrated equipment structure, the extraction efficiency of the lumbrukinase is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of bioactive substance extraction, and in particular to a highly active lumbrokinase extraction device and method. Background Art

[0002] Earthworms are an indispensable part of nature. Ancient Chinese people recognized their medicinal value. Called earthworms in Traditional Chinese Medicine, they are known for their cold and salty properties, and possess heat-clearing, antispasmodic, and antiasthmatic properties. Modern research has revealed that lumbrokinase, an enzyme mixture isolated from earthworms, possesses a unique affinity for blood clots. It effectively dissolves microemboli, improves microcirculation, strengthens cardiovascular and cerebral collateral circulation, repairs damaged endothelial cells, increases vascular elasticity, improves vascular oxygenation, reduces blood viscosity, decreases platelet aggregation, and inhibits re-thrombosis. It also repairs necrotic brain cells surrounding thrombi and salvages penumbras. It is currently widely used clinically to treat cardiovascular, endocrine, and respiratory conditions.

[0003] However, in the existing technology, the extraction purity and yield of lumbrokinase are still low, the preparation steps are complicated, and the production efficiency is low; moreover, during the lumbrokinase extraction process, the cleaning, grinding and filtration separation of earthworms are all carried out separately, and material transfer and waiting are required between each step, resulting in low extraction efficiency, affecting the activity of lumbrokinase, and the filtered earthworm residue will still contain some extract, resulting in waste of raw materials. Summary of the Invention

[0004] In response to the above-mentioned technical problems, the present invention provides a high-activity lumbrokinase extraction device and method.

[0005] The technical solution of the present invention is: a high-activity lumbrokinase extraction device, comprising an equipment bracket, a cleaning assembly arranged at the upper end of the equipment bracket, and a grinding assembly and a separation assembly arranged at the lower end of the equipment bracket and respectively located below the cleaning assembly; the equipment bracket comprises a base and a vertical plate arranged at the rear side of the upper end surface of the base;

[0006] The cleaning assembly includes a first horizontal plate arranged at the upper end of the outer side wall of the vertical plate, a cleaning outer cylinder arranged on the upper end surface of the first horizontal plate, and an inner net cylinder sleeved inside the cleaning outer cylinder; a sewage pipe is arranged at the lower end of the outer side wall of the cleaning outer cylinder; a pressure cover is movably connected to the top of the cleaning outer cylinder, a cleaning coil is arranged at the top of the pressure cover, and a water inlet pipe connected to the cleaning coil is arranged at the top of the pressure cover; a sliding rod is arranged at the bottom of the inner net cylinder, which passes through the pressure cover, and a compression spring is sleeved on the sliding rod to abut against the top of the pressure cover;

[0007] The grinding assembly includes a second horizontal plate arranged on the outer side wall of the vertical plate and located below the first horizontal plate, a grinding seat arranged on the upper end surface of the second horizontal plate, a rotating seat rotatably clamped inside the grinding seat, and a grinding motor arranged on the top of the grinding seat and providing power for the grinding seat; a blanking hole is provided on the second horizontal plate below the gap between the grinding seat and the rotating seat, a collecting bucket is provided on the lower bottom surface of the second horizontal plate and located outside the blanking hole, and a transfer pipe is provided at the lower end of the collecting bucket; a guide sleeve is provided on the upper end surface of the grinding seat; the rotating seat is a frustum structure with a larger upper portion and a smaller lower portion, and a plurality of grinding rollers are equidistantly distributed circumferentially on the outer side wall of the rotating seat; the inner cavity of the grinding seat is adapted to the shape of the rotating seat; the grinding motor is arranged on the top of the guide sleeve through a frame, and the output end of the grinding motor is connected to the rotating seat;

[0008] The separation component includes a separation cylinder arranged on the upper end surface of the base, several conical separation mesh covers equidistantly distributed inside the separation cylinder, material-discharging blades arranged inside the separation cylinder and respectively located above each conical separation mesh cover, and a material-discharging motor arranged at the top of the separation cylinder and simultaneously providing power for each material-discharging blade; a feed pipe is provided at the top of the separation cylinder, and a collection pipe is provided at the lower end of the outer side wall of the separation cylinder; the mesh diameter of each conical separation mesh cover decreases successively from top to bottom; the output end of the material-discharging motor is provided with a drive shaft passing through each conical separation mesh cover, and each material-discharging blade is respectively sleeved on the drive shaft.

[0009] Furthermore, a universal ball seat is provided at the bottom end of the cleaning outer cylinder; a supporting ball head movably engaged with the universal ball seat is provided on the upper end surface of the first horizontal plate, and a rotating sleeve is provided at the bottom end of the cleaning outer cylinder and located outside the universal ball seat, and a rotating connecting seat is slidably engaged with the rotating sleeve; a swing sleeve is provided on the upper end surface of the first horizontal plate, and an arc-shaped swing disk is provided on the inner side wall of the swing sleeve, and a swing push rod is slidably engaged with the arc-shaped swing disk; a rotating ring is provided on the upper end surface of the first horizontal plate, and an inner gear ring is provided on the inner side of the rotating ring; a push plate movably hingedly connected to the rotating ring and the rotating connecting seat respectively; a swing motor is provided on the lower bottom surface of the first horizontal plate, and a small gear meshing with the inner gear ring is provided at the output end of the swing motor;

[0010] Description: During the earthworm cleaning process, the swing motor is used to drive the pinion to rotate, and the meshing action of the pinion and the inner gear ring is used to drive the rotating circle to rotate. During the rotation of the rotating circle, the push plate is driven to rotate. At this time, the swing push rod swings back and forth inside the arc-shaped swing disk, and when the rotating connecting seat rotates inside the rotating sleeve, the cleaning outer cylinder is pushed by the swing push rod to swing back and forth on the upper end of the supporting ball head, which is beneficial to improving the cleaning effect of the earthworms and improving the purity of lumbrokinase.

[0011] Furthermore, the first horizontal plate is rotatably engaged with the vertical plate, and a movable cylinder cover communicating with the interior of the cleaning outer cylinder is provided at the lower end of the outer wall of the cleaning outer cylinder;

[0012] Description: By rotating and locking the first horizontal plate and the vertical plate, it is convenient to transfer the cleaned earthworms to the inside of the grinding seat through the movable cylinder cover.

[0013] Furthermore, the transfer pipe is connected to a circulation pipe through a three-way valve, and the other end of the circulation pipe is connected to the inner cavity of the grinding seat; a circulation pump connected to the circulation pipe is provided on the second horizontal plate; another port of the three-way valve is connected to an extension pipe, which is connected to the feed pipe, and a feed pump connected to the extension pipe is provided on the base;

[0014] Description: The circulation pump facilitates repeated crushing and grinding of earthworms, which is beneficial to improving the grinding effect of earthworms and thus promoting the extraction of lumbrokinase in earthworms; and the feed pump is set to facilitate the transfer of ground earthworm tissues.

[0015] Furthermore, a plurality of adjustment seats are evenly distributed on the outer wall of the rotating seat, and each grinding roller is slidably engaged with each adjustment seat in a one-to-one correspondence. A return spring is provided inside the rotating seat and abuts against each adjustment seat respectively; a push slide is provided inside the rotating seat and abuts against each adjustment seat at the same time, and a first electric rod connected to the push slide is provided at the top of the rotating seat;

[0016] Description: The first electric rod is used to push the slide downward inside the rotating seat. At this time, the adjusting seat can drive the corresponding grinding roller close to the inner wall of the grinding seat, thereby improving the degree of refinement of the earthworms.

[0017] Furthermore, the inner wall of the grinding seat is provided with first extrusion teeth, and the outer wall of each grinding roller is provided with second extrusion teeth;

[0018] Note: The cooperation between the second extrusion teeth and the first extrusion teeth is beneficial to improving the crushing and grinding efficiency of earthworms.

[0019] Furthermore, a sliding sleeve is sleeved on the outside of the drive shaft, and each conical separation screen is sleeved on the outside of the sliding sleeve; a damping spring is sleeved on the lower end of the drive shaft and abuts against the sliding sleeve, a docking plate is slidably engaged with the upper end of the drive shaft, and a compression screw is threadedly connected to the separation cylinder and slidably engaged with the docking plate; each conical separation screen is rotatably engaged with the inner wall of the separation cylinder;

[0020] Description: After the earthworm tissue separation is completed, the compression screw is used to push the docking plate downward along the drive shaft, and the material-diverting fan blade drives the sliding sleeve to slide downward along the drive shaft, and finally the material-diverting fan blade is engaged with the conical separation mesh cover. At this time, the material-diverting fan blade and the conical separation mesh cover are driven by the drive shaft to rotate at the same time, which facilitates the centrifugal treatment of the earthworm tissue blocked by the conical separation mesh cover, making the separation of effective substances in the earthworm tissue more thorough.

[0021] Furthermore, a cleaning door is movably hinged on the outer side wall of the separation cylinder;

[0022] Note: The cleaning door is provided to facilitate the cleaning of earthworm tissues blocked by the conical separation mesh, thus improving the convenience of using the equipment.

[0023] Furthermore, a conical blanking platform is provided on the top of the rotating seat;

[0024] Note: By setting up a conical drop table, the cleaned earthworms can quickly fall into the grinding seat, which is beneficial to improving the working efficiency of the equipment.

[0025] The present invention also provides a method for extracting high-activity lumbrokinase, based on the above-mentioned high-activity lumbrokinase extraction device, comprising the following steps:

[0026] S1. Place the adult earthworms into the inner net cylinder. The gland slides downward along the sliding rod under the action of the compression spring and is clamped on the top of the cleaning outer cylinder. Then, external clean water is introduced into the cleaning coil through the water inlet pipe to clean the adult earthworms. The wastewater generated during the cleaning process is discharged through the sewage pipe.

[0027] S2. After the adult earthworms are cleaned, the adult earthworms are placed into the grinding seat through the guide sleeve. The grinding motor drives the rotating seat to rotate inside the grinding seat. The adult earthworms are ground and crushed by the squeezing effect between the grinding roller and the inner wall of the grinding seat to obtain earthworm slurry. The earthworm slurry enters the collection bucket through the drop hole and is discharged through the transfer pipe.

[0028] S3. Place the earthworm slurry into the separation cylinder through the feed pipe. After the earthworm slurry is filtered and separated by each conical separation mesh cover, liquid lumbrokinase is obtained and discharged through the collection tube. After the liquid lumbrokinase is freeze-dried, lumbrokinase powder can be obtained. During the earthworm slurry separation process, the driving shaft is driven to rotate by the feeding motor. At this time, each feeding fan blade rotates above the corresponding conical separation mesh cover to stir the earthworm slurry.

[0029] Compared with the prior art, the beneficial effects of the present invention are embodied in the following aspects:

[0030] First, the device structure of the present invention is rationally designed. By cleaning, grinding and separating the adult earthworms, the lumbrokinase in the earthworms is completely separated, effectively improving the purity and activity of the lumbrokinase. At the same time, the integrated device structure of the present invention greatly improves the extraction efficiency of the lumbrokinase.

[0031] Second, the cleaning assembly of the present invention can swing back and forth during use, so that the adult earthworms inside the inner net tube are always in motion. This not only improves the cleaning effect of the adult earthworms and lays a foundation for improving the purity of lumbrokinase, but also avoids damage to the adult earthworms caused by traditional cleaning methods.

[0032] Third, the present invention performs multi-stage filtration separation and centrifugal separation on the ground earthworm slurry, so that the lumbrokinase in the earthworm slurry can be completely released, which is beneficial to improving the utilization rate of raw materials and reducing the production cost of lumbrokinase. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is a longitudinal sectional view of the apparatus of the present invention;

[0034] Figure 2 It is a right side view of the device of the present invention;

[0035] Figure 3 This invention Figure 1 A partial enlarged schematic diagram of point A in the middle;

[0036] Figure 4 This is a schematic diagram of the connection between the inner net cylinder and the cleaning outer cylinder of the present invention;

[0037] Figure 5 This is a schematic diagram of the connection between the swing push rod and the arc-shaped swing plate of the present invention;

[0038] Figure 6 This is a schematic diagram of the connection between the grinding roller and the rotating seat of the present invention;

[0039] Figure 7 This is a distribution diagram of the grinding rollers of the present invention on the rotating seat;

[0040] Figure 8 Schematic diagram of the internal structure of the separation cylinder of the present invention;

[0041] Among them, 1- equipment bracket, 10- base, 11- vertical plate, 2- cleaning assembly, 20- first horizontal plate, 21- cleaning outer cylinder, 210- drain pipe, 211- pressure cover, 212- universal ball seat, 213- support ball head, 214- rotating sleeve, 215- rotating connecting seat, 216- movable cylinder cover, 22- inner net cylinder, 220- sliding rod, 221- compression spring, 23- cleaning coil, 230- water inlet pipe, 24- swing sleeve, 240- arc swing disk, 241- swing push rod, 242- push plate, 25- rotating ring, 250- inner gear ring, 26- swing motor, 260- small gear, 3- grinding assembly, 30- second horizontal plate, 300- drop hole, 31- grinding seat, 310 -guide sleeve, 311-first extrusion tooth, 32-rotating seat, 320-grinding roller, 3200-second extrusion tooth, 321-adjusting seat, 322-reset spring, 323-conical blanking table, 33-grinding motor, 34-collecting bucket, 340-transfer pipe, 341-circulation pipe, 342-extension pipe, 35-circulation pump, 36-pushing slide, 37-first electric rod, 4-separation assembly, 40-separation cylinder, 400-feed pipe, 401-collection pipe, 402-cleaning door, 41-conical separation mesh cover, 42-feeding fan blade, 43-feeding motor, 430-drive shaft, 431-sliding sleeve, 432-damping spring, 433-docking plate, 44-feeding pump, 45-compression screw. DETAILED DESCRIPTION

[0042] Example 1

[0043] like Figure 1 、 2 The device for extracting high-activity lumbrokinase shown in the figure includes an equipment support 1, a cleaning assembly 2 disposed at the upper end of the equipment support 1, and a grinding assembly 3 and a separation assembly 4 disposed at the lower end of the equipment support 1 and respectively located below the cleaning assembly 2. The equipment support 1 includes a base 10 and a vertical plate 11 disposed on the rear side of the upper end surface of the base 10.

[0044] like Figure 1 、 4 As shown, the cleaning assembly 2 includes a first horizontal plate 20 arranged at the upper end of the outer wall of the vertical plate 11, a cleaning outer cylinder 21 arranged on the upper end surface of the first horizontal plate 20, and an inner net cylinder 22 sleeved inside the cleaning outer cylinder 21; a sewage pipe 210 is provided at the lower end of the outer wall of the cleaning outer cylinder 21; a pressure cover 211 is movably connected to the top of the cleaning outer cylinder 21, a cleaning coil 23 is provided at the top of the pressure cover 211, and a water inlet pipe 230 connected to the cleaning coil 23 is provided at the top of the pressure cover 211; a sliding rod 220 is provided at the bottom of the inner net cylinder 22, which passes through the pressure cover 211, and a compression spring 221 is sleeved on the sliding rod 220 and abuts against the top of the pressure cover 211;

[0045] like Figure 1As shown, the grinding assembly 3 includes a second transverse plate 30 arranged on the outer wall of the vertical plate 11 and located below the first transverse plate 20, a grinding seat 31 arranged on the upper end surface of the second transverse plate 30, a rotating seat 32 rotatably engaged with the grinding seat 31, and a grinding motor 33 arranged at the top of the grinding seat 31 and providing power for the grinding seat 31; a blanking hole 300 is provided on the second transverse plate 30 and is located below the gap between the grinding seat 31 and the rotating seat 32; a collecting bucket 34 is provided on the lower bottom surface of the second transverse plate 30 and is located outside the blanking hole 200, and a transfer pipe 340 is provided at the lower end of the collecting bucket 34; a guide sleeve 310 is provided on the upper end surface of the grinding seat 31; the rotating seat 32 is a frustum structure with a larger upper portion and a smaller lower portion, and four grinding rollers 320 are equidistantly distributed circumferentially on the outer side wall of the rotating seat 32; the inner cavity of the grinding seat 31 is adapted to the shape of the rotating seat 32; the grinding motor 33 is arranged at the top inside the guide sleeve 310 through a frame, and the output end of the grinding motor 33 is connected to the rotating seat 32;

[0046] like Figure 2 、 8 As shown, the separation assembly 4 includes a separation cylinder 40 arranged on the upper end surface of the base 10, three conical separation screens 41 equidistantly distributed inside the separation cylinder 40, a material-dispensing fan blade 42 arranged inside the separation cylinder 40 and respectively located above each conical separation screen 41, and a material-dispensing motor 43 arranged at the top of the separation cylinder 40 and simultaneously providing power for each material-dispensing fan blade 42; a feed pipe 400 is provided at the top of the separation cylinder 40, and a collection pipe 401 is provided at the lower end of the outer wall of the separation cylinder 40; the mesh diameter of each conical separation screen 41 decreases from top to bottom, and is 0.8 mm, 0.5 mm, and 0.2 mm respectively; a drive shaft 430 is provided at the output end of the material-dispensing motor 43, which passes through each conical separation screen 41, and each material-dispensing fan blade 42 is respectively sleeved on the drive shaft 430;

[0047] In this embodiment, the grinding motor 33 and the material-discharging motor 43 are both products in the prior art, for example, they can both be gear reduction motors produced by Dongguan Yutian Motor Co., Ltd.

[0048] Example 2

[0049] This embodiment describes a method for extracting high-activity lumbrokinase, based on a high-activity lumbrokinase extraction device according to Example 1, comprising the following steps:

[0050] S1. Place the adult earthworms into the inner net cylinder 22. The gland 211 slides downward along the sliding rod 220 under the action of the compression spring 221 and is clamped on the top of the cleaning outer cylinder 21. Then, external clean water is introduced into the cleaning coil 23 through the water inlet pipe 230 to clean the adult earthworms. The wastewater generated during the cleaning process is discharged through the sewage pipe 210.

[0051] S2. After the adult earthworms are cleaned, the adult earthworms are placed into the grinding seat 31 through the guide sleeve 310. The grinding motor 33 drives the rotating seat 32 to rotate inside the grinding seat 31. The grinding roller 320 and the inner wall of the grinding seat 31 are squeezed to grind and crush the adult earthworms to obtain earthworm slurry. The earthworm slurry enters the collecting hopper 34 through the drop hole 300 and is discharged through the transfer pipe 340.

[0052] S3. Place the earthworm slurry into the separation cylinder 40 through the feed pipe 400. After the earthworm slurry is filtered and separated by each conical separation mesh cover 41, liquid lumbrokinase is obtained and discharged through the collection tube 401. After the liquid lumbrokinase is freeze-dried at a temperature of -18°C, lumbrokinase powder can be obtained. During the earthworm slurry separation process, the driving shaft 430 is driven to rotate by the feeding motor 43. At this time, each feeding fan blade 42 rotates above the corresponding conical separation mesh cover 41 to stir the earthworm slurry.

[0053] Example 3

[0054] This embodiment differs from embodiment 1 in that:

[0055] like Figure 1 、 3 As shown, a universal ball seat 212 is provided at the bottom end of the cleaning outer cylinder 21; a supporting ball head 213 movably engaged with the universal ball seat 212 is provided on the upper end surface of the first horizontal plate 20, a rotating clamping sleeve 214 is provided at the bottom end of the cleaning outer cylinder 21 and located outside the universal ball seat 212, and a rotating connecting seat 215 is slidably engaged on the rotating clamping sleeve 214; a swinging sleeve 24 is provided on the upper end surface of the first horizontal plate 20, and an arc-shaped swinging disc 240 is provided on the inner side wall of the swinging sleeve 24, and the arc-shaped swinging disc 240 slides upward. The movable clamp is connected with a swing push rod 241; the upper end surface of the first horizontal plate 20 is provided with a rotating ring 25 which is sleeved on the inner side of the swing sleeve 24, and the inner side of the rotating ring 25 is provided with an inner gear ring 250; the swing push rod 241 is movably hinged with a push plate 242 which is movably hinged to the rotating ring 25 and the rotating connecting seat 215 respectively; the lower bottom surface of the first horizontal plate 20 is provided with a swing motor 26 (commercially available product), and the output end of the swing motor 26 is provided with a small gear 260 which is meshed with the inner gear ring 250.

[0056] Example 4

[0057] This embodiment differs from embodiment 3 in that:

[0058] like Figure 1 、 2 As shown, the first horizontal plate 20 is rotatably connected to the vertical plate 11, and a movable cylinder cover 216 which is connected to the interior of the cleaning outer cylinder 21 is provided at the lower end of the outer side wall of the cleaning outer cylinder 21; by rotatably connecting the first horizontal plate 20 to the vertical plate 11, it is convenient to transfer the cleaned earthworms to the interior of the grinding seat 31 through the movable cylinder cover 216.

[0059] Example 5

[0060] This embodiment differs from embodiment 4 in that:

[0061] like Figure 2 As shown, the transfer tube 340 is connected to a circulation tube 341 through a three-way valve (commercially available product), and the other end of the circulation tube 341 is connected to the inner cavity of the grinding seat 31; a circulation pump 35 connected to the circulation tube 341 is provided on the second horizontal plate 30; another port of the three-way valve is connected to an extension tube 342, and the extension tube 342 is connected to the feed tube 400, and a feed pump 44 connected to the extension tube 342 is provided on the base 10; the circulation pump 35 facilitates repeated crushing and grinding of earthworms, which is beneficial to improving the grinding effect of earthworms, thereby promoting the extraction effect of lumbrokinase in earthworms; and the feed pump 44 is provided to facilitate the transfer of ground earthworm tissue.

[0062] In this embodiment, the circulation pump 35 and the feed pump 44 are both products of the prior art, for example, a PYSP310-XC miniature diaphragm water pump produced by Shenzhen Pincheng Motor Co., Ltd. can be used;

[0063] Example 6

[0064] This embodiment differs from embodiment 5 in that:

[0065] like Figure 6 As shown, four adjustment seats 321 are evenly distributed on the outer wall of the rotating seat 32, and each grinding roller 320 is slidably engaged with each adjustment seat 321 one by one. A return spring 322 is provided inside the rotating seat 32 to abut against each adjustment seat 321 respectively; a pushing slide 36 is provided inside the rotating seat 32 to abut against each adjustment seat 321 at the same time, and a first electric rod 37 (commercially available product) connected to the pushing slide 36 is provided at the top of the rotating seat 32.

[0066] Example 7

[0067] This embodiment differs from embodiment 6 in that:

[0068] like Figure 7 As shown, a first extrusion tooth 311 is provided on the inner wall of the grinding seat 31, and a second extrusion tooth 3200 is provided on the outer wall of each grinding roller 320; the cooperation between the second extrusion teeth 3200 and the first extrusion teeth 311 is beneficial to improving the crushing and grinding efficiency of earthworms.

[0069] Example 8

[0070] This embodiment differs from embodiment 7 in that:

[0071] like Figure 2 、8 As shown, a sliding sleeve 431 is sleeved on the outside of the driving shaft 430, and each conical separation mesh cover 41 is sleeved on the outside of the sliding sleeve 431; a damping spring 432 is sleeved on the lower end of the driving shaft 430 and abuts against the sliding sleeve 431, and a docking plate 433 is slidably engaged with the upper end of the driving shaft 430, and a clamping screw 45 is threadedly connected to the separation cylinder 40 and slidably engaged with the docking plate 433; each conical separation mesh cover 41 is rotatably engaged with the inner wall of the separation cylinder 40; a cleaning door 402 is movably hinged on the outer wall of the separation cylinder 40.

[0072] Example 9

[0073] The difference between this embodiment and embodiment 8 is that:

[0074] like Figure 6 As shown, a conical drop-out platform 323 is provided at the top of the rotating seat 32; by providing the conical drop-out platform 323, the cleaned earthworms can quickly fall into the grinding seat 31, which is beneficial to improving the working efficiency of the equipment.

[0075] Example 10

[0076] This example describes a method for extracting high-activity lumbrokinase, based on a high-activity lumbrokinase extraction device according to Example 9, comprising the following steps:

[0077] S1. Place the adult earthworms into the inner net cylinder 22. The pressure cover 211 slides downward along the sliding rod 220 under the action of the compression spring 221 and is clamped on the top of the cleaning outer cylinder 21. Then, the external clean water is introduced into the cleaning coil 23 through the water inlet pipe 230 to clean the adult earthworms. The waste water generated during the cleaning process is discharged through the sewage pipe 210. During the cleaning process, the swing motor 26 is used to drive the pinion 260 to rotate, and the meshing action of the pinion 260 and the inner gear ring 250 is used to drive the rotating ring 25 to rotate. During the rotation of the rotating ring 25, the push plate 242 is driven to rotate. At this time, the swing push rod 241 swings back and forth inside the arc-shaped swing disk 240, and when the rotating connecting seat 215 rotates inside the rotating sleeve 214, the outer cleaning cylinder 21 is pushed by the swing push rod 241 to swing back and forth on the upper end of the supporting ball head 213.

[0078] S2. After the adult earthworms are cleaned, the adult earthworms are placed into the grinding seat 31 through the guide sleeve 310. The grinding motor 33 drives the rotating seat 32 to rotate inside the grinding seat 31. The grinding roller 320 and the inner wall of the grinding seat 31 are squeezed to grind and crush the adult earthworms to obtain earthworm slurry. The earthworm slurry enters the collecting bucket 34 through the drop hole 300 and is discharged through the transfer pipe 340. During the grinding process of the adult earthworms, the first electric rod 37 is used to push the slide 36 downward inside the rotating seat 32. At this time, the adjusting seat 321 can drive the corresponding grinding roller 320 to approach the inner wall of the grinding seat 31.

[0079] S3, the earthworm slurry is placed into the separation cylinder 40 through the feed pipe 400, and the earthworm slurry is filtered and separated by each conical separation mesh cover 41 to obtain liquid lumbrokinase, which is then discharged through the collection tube 401. The liquid lumbrokinase is freeze-dried to obtain lumbrokinase powder; during the separation process of the earthworm slurry, the driving shaft 430 is driven to rotate by the feeding motor 43, and at this time, each feeding fan blade 42 rotates above the corresponding conical separation mesh cover 41 to stir the earthworm slurry; After the earthworm slurry separation is completed, the compression screw 45 is used to push the docking plate 433 to move downward along the drive shaft 430, and the material-diverting fan blade 42 drives the sliding sleeve 431 to slide downward along the drive shaft 430, and finally the material-diverting fan blade 42 is engaged with the conical separation mesh cover 41. At this time, the material-diverting fan blade 42 and the conical separation mesh cover 41 are driven by the drive shaft 430 to rotate at the same time, and the earthworm tissue blocked by the conical separation mesh cover 41 is centrifuged, so that the effective substances in the earthworm tissue are fully separated.

Claims

1. A highly active lumbrokinase extraction device, characterized in that: The device comprises an equipment support (1), a cleaning assembly (2) arranged at the upper end of the equipment support (1), and a grinding assembly (3) and a separation assembly (4) arranged at the lower end of the equipment support (1) and respectively located below the cleaning assembly (2); the equipment support (1) comprises a base (10) and a vertical plate (11) arranged at the rear side of the upper end surface of the base (10); The cleaning assembly (2) comprises a first transverse plate (20) arranged at the upper end of the outer side wall of the vertical plate (11), a cleaning outer cylinder (21) arranged on the upper end surface of the first transverse plate (20), and an inner net cylinder (22) sleeved inside the cleaning outer cylinder (21); a pressure cover (211) is movably connected to the top of the cleaning outer cylinder (21), and a cleaning coil (23) is arranged at the top of the pressure cover (211); The grinding assembly (3) comprises a second transverse plate (30) arranged on the outer wall of the vertical plate (11), a grinding seat (31) arranged on the upper end surface of the second transverse plate (30), a rotating seat (32) rotatably connected to the inside of the grinding seat (31), and a grinding motor (33) arranged at the top of the grinding seat (31) and providing power to the grinding seat (31); a blanking hole (300) located below the gap between the grinding seat (31) and the rotating seat (32) is provided through the second transverse plate (30); a collecting hopper (34) located outside the blanking hole (200) is provided on the lower bottom surface of the second transverse plate (30), and a transfer pipe (340) is provided at the lower end of the collecting hopper (34); a plurality of grinding rollers (320) are equidistantly distributed circumferentially on the outer wall of the rotating seat (32); The separation assembly (4) comprises a separation cylinder (40) arranged on the upper end surface of the base (10), a plurality of conical separation screens (41) equidistantly distributed inside the separation cylinder (40), a material-dispensing fan blade (42) arranged inside the separation cylinder (40) and respectively located above each of the conical separation screens (41), and a material-dispensing motor (43) arranged at the top of the separation cylinder (40) and simultaneously providing power to each of the material-dispensing fan blades (42); the output end of the material-dispensing motor (43) is provided with a drive shaft (430) that passes through each of the conical separation screens (41).

2. The high-activity lumbrokinase extraction device according to claim 1, characterized in that: The bottom end of the cleaning outer cylinder (21) is provided with a universal ball seat (212); the upper end surface of the first horizontal plate (20) is provided with a supporting ball head (213) movably engaged with the universal ball seat (212); the bottom end of the cleaning outer cylinder (21) and located outside the universal ball seat (212) is provided with a rotating sleeve (214), and a rotating connecting seat (215) is slidably engaged on the rotating sleeve (214); the upper end surface of the first horizontal plate (20) is provided with a swing sleeve (24), and an arc-shaped swing disk (240) is provided on the inner side wall of the swing sleeve (24), and the arc-shaped swing disk (240) is provided. The upper sliding clamp is provided with a swing push rod (241); the upper end surface of the first transverse plate (20) is provided with a rotating ring (25) sleeved on the inner side of the swing sleeve (24), and the inner side of the rotating ring (25) is provided with an inner gear ring (250); the swing push rod (241) is movably hinged with a push plate (242) movably hinged to the rotating ring (25) and the rotating connecting seat (215); the lower bottom surface of the first transverse plate (20) is provided with a swing motor (26), and the output end of the swing motor (26) is provided with a small gear (260) meshing with the inner gear ring (250).

3. The high-activity lumbrokinase extraction device according to claim 1, characterized in that: The transfer pipe (340) is connected to a circulation pipe (341) via a three-way valve, and the other end of the circulation pipe (341) is communicated with the inner cavity of the grinding seat (31); a circulation pump (35) connected to the circulation pipe (341) is provided on the second horizontal plate (30); another port of the three-way valve is connected to an extension pipe (342), and the extension pipe (342) is connected to the separation cylinder (40), and a feed pump (44) connected to the extension pipe (342) is provided on the base (10).

4. The high-activity lumbrokinase extraction device according to claim 1, characterized in that: A plurality of adjustment seats (321) are evenly distributed on the outer wall of the rotating seat (32), and each of the grinding rollers (320) is slidably engaged with each of the adjustment seats (321) in a one-to-one correspondence. A return spring (322) is provided inside the rotating seat (32) and is respectively in contact with each of the adjustment seats (321); a push slide (36) is provided inside the rotating seat (32) and is simultaneously in contact with each of the adjustment seats (321). A first electric rod (37) connected to the push slide (36) is provided on the top of the rotating seat (32).

5. The high-activity lumbrokinase extraction device according to claim 1, characterized in that: The driving shaft (430) is sleeved with a sliding sleeve (431) on the outside, and each of the conical separation net covers (41) is sleeved on the outside of the sliding sleeve (431); the lower end of the driving shaft (430) is sleeved with a damping spring (432) that abuts against the sliding sleeve (431); the upper end of the driving shaft (430) is slidably engaged with a docking plate (433); the separation cylinder (40) is threadedly connected with a compression screw (45) that is slidably engaged with the docking plate (433); and each of the conical separation net covers (41) is rotatably engaged with the inner wall of the separation cylinder (40).

6. The high-activity lumbrokinase extraction device according to claim 1, characterized in that: A conical blanking platform (323) is provided at the top of the rotating seat (32).

7. A method for extracting high-activity lumbrokinase, based on the high-activity lumbrokinase extraction device according to claim 2, characterized in that: The following steps are involved: S1. Place the adult earthworms into the inner net cylinder (22), then clamp the pressure cover (211) on the top of the cleaning outer cylinder (21), and finally pass external clean water into the cleaning coil (23) to clean the adult earthworms. During the cleaning process, the swing motor (26) is used to drive the small gear (260) to rotate, and the meshing action of the small gear (260) and the inner gear ring (250) is used to drive the rotating ring (25) to rotate. During the rotation of the rotating ring (25), the push plate (242) is driven to rotate. At this time, the swing push rod (241) swings back and forth inside the arc-shaped swing disk (240), and when the rotating connecting seat (215) rotates inside the rotating sleeve (214), the outer cleaning cylinder (21) is pushed by the swing push rod (241) to swing back and forth on the upper end of the supporting ball head (213); S2. After the adult earthworms are cleaned, the adult earthworms are placed inside the grinding seat (31), and the grinding motor (33) drives the rotating seat (32) to rotate inside the grinding seat (31). The adult earthworms are ground and crushed by the squeezing effect between the grinding roller (320) and the inner wall of the grinding seat (31), thereby obtaining an earthworm slurry; The earthworm slurry enters the collecting hopper (34) through the drop hole (300) and is discharged through the transfer pipe (340); S3. Place the earthworm slurry into the earthworm slurry. After the earthworm slurry is filtered and separated by each conical separation mesh cover (41), liquid lumbrokinase is obtained. After the liquid lumbrokinase is freeze-dried, lumbrokinase powder can be obtained. During the earthworm slurry separation process, the driving shaft (430) is driven to rotate by the material-diverting motor (43). At this time, each material-diverting fan blade (42) rotates above the corresponding conical separation mesh cover (41) to dig the earthworm slurry.

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