Chiral molecule configuration recognition method and device and application

Abstract

The invention provides a chiral molecular configuration recognition method, a chiral molecular configuration recognition device and application. The chiral molecular configuration recognition method comprises the following steps: alternately depositing two or more materials with different refractive indexes on a substrate to form a reflector substrate with a periodic multilayer film structure; the method comprises the following steps: depositing metal chiral nanoparticles on a reflector substrate to obtain a chiral molecule detection sensor, and immersing the chiral molecule detection sensor in a to-be-detected chiral molecule solution; the method comprises the following steps: irradiating a chiral molecule detection sensor immersed in a to-be-detected chiral molecule solution with left-handed circularly polarized light LCP and right-handed circularly polarized light RCP to obtain a corresponding circular differential scattering spectrum, and analyzing spectrum peak position offset characteristics to realize judgment on left-handed or right-handed molecules of the to-be-detected chiral molecule solution. The method is high in sensitivity, short in detection time, label-free, washing-free and free of chiral antibodies, and has extremely high market value and application prospect.

Description

Technical Field

[0001] The present invention belongs to the technical field of chiral molecule detection, and particularly relates to a method, device and application for identifying the configuration of a chiral molecule. Background Art

[0002] Chiral molecule detection holds immense scientific significance and broad application prospects. Chirality, the property that a molecule's structure cannot be superimposed on its mirror image, is widely found in pharmaceuticals, food, agriculture, biomedicine, and other fields. The two enantiomers of chiral molecules (L- and D-forms) often exhibit significant differences in efficacy, toxicity, and metabolic pathways. For example, in the thalidomide case, the L-form exhibited a sedative effect, while the D-form at a concentration of 10 μM could cause severe fetal malformations. The L-form of epinephrine is 30 times more active than the D-form. The food sweetener aspartame is sweet only in its L-form, while the D-form is tasteless or even bitter. Therefore, chiral recognition and quantitative detection are key technologies for new drug development, clinical drug safety, food safety inspections, and environmental hormone monitoring. Chiral sensing has also become a core tool for early disease warning and precision medicine.

[0003] The current mainstream technology still faces many bottlenecks and is difficult to meet the needs of modern analytical chemistry. Traditional methods such as high performance liquid chromatography (HPLC) require chiral chromatographic columns, are time-consuming, and have low sensitivity; nuclear magnetic resonance equipment is expensive, requires a large amount of samples, and is complex to operate; circular dichroism (CD) is a classic chiral optical method with a high detection limit (10 ⁻4 Chiral sensing is often characterized by high sensitivity, high sensitivity, and low versatility, making it difficult to detect trace amounts of chiral molecules (such as pesticide residues and chiral blood markers). While nanotechnology (SERS and chiral metamaterials) has attempted to overcome these barriers over the past decade, they still suffer from unstable signals, insufficient sensitivity, and limited versatility. Consequently, the field of chiral sensing urgently needs a new detection paradigm that is ultrasensitive, rapid, universal, and inexpensive. Summary of the Invention

[0004] One object of the present invention is to address the shortcomings of the existing technology and provide a method for identifying the configuration of chiral molecules. The method has high sensitivity, short detection time, is label-free, wash-free, and does not require chiral antibodies, and has extremely high market value and application prospects.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions: A method for identifying the configuration of a chiral molecule comprises the following steps: A reflector substrate having a periodic multilayer film structure is formed by alternately depositing two or more materials with different refractive indices on a substrate; Depositing metal chiral nanoparticles on a reflector substrate to obtain a chiral molecule detection sensor, and immersing the chiral molecule detection sensor in a solution of chiral molecules to be detected; The chiral molecule detection sensor immersed in the chiral molecule solution to be detected is irradiated with left-handed circularly polarized light (LCP) and right-handed circularly polarized light (RCP), and the corresponding circular differential scattering spectra are obtained. The spectral peak shift characteristics are analyzed to determine the left-handed or right-handed configuration of the chiral molecule solution to be detected.

[0006] Furthermore, in step 1, the refractive index difference between the two materials of adjacent film layers is 0.2-1.

[0007] Furthermore, the high refractive index material in the reflector substrate is 、ZnS、 or One or more of the following, the low refractive index material is a polymer, 、 or One or more of .

[0008] Furthermore, the number of film layers of the reflector substrate in step 1 is 5-20 layers, and the thickness of each film layer is / ,in, is the central wavelength, is the refractive index of the film material.

[0009] Furthermore, in step 2, the metal chiral nanoparticles are one or more chiral nanoparticle structures based on gold, silver or copper.

[0010] Another object of the present invention is to provide a device for the above-mentioned method of identifying the configuration of chiral molecules, comprising a chiral molecule detection sensor and an optical detection system, wherein: A chiral molecule detection sensor comprises a reflector substrate with a periodic multilayer film structure and metal chiral nanoparticles deposited on the reflector substrate, wherein the refractive indices of adjacent film layers on the reflector substrate are different; An optical detection system comprising a light source, a linear polarizer, a wave plate, a microscope objective lens, an objective lens, a spectrometer, and a data processing unit sequentially arranged on an optical path; When performing chirality detection, the chiral molecule detection sensor is immersed in the chiral molecule solution to be detected. The light generated by the light source is focused by the microscope objective lens onto the surface of the chiral molecule detection sensor in the solution after passing through the linear polarizer and wave plate. The signal scattered by the chiral molecule detection sensor is collected by the objective lens and sent to the spectrometer. The data processing unit receives the spectral signal scattered by the spectrometer and processes it to obtain the chiral configuration of the chiral molecule solution to be detected.

[0011] Furthermore, the central wavelength range of the reflection spectrum of the reflector substrate is 400-1000 nm, and the coupling wavelength range of the surface plasmon resonance peak of the metal chiral nanoparticles and the reflector substrate is 500-900 nm.

[0012] Furthermore, the angle difference between the fast axis angle of the polarizer and the fast axis angle of the wave plate is 90°, which is used to generate left-handed circularly polarized light LCP and right-handed circularly polarized light RCP respectively.

[0013] The present invention also provides an application of an identification device based on the above-mentioned chiral molecular configuration, and the device is used in clinical medicine and disease diagnosis, environmental protection, pesticide residue monitoring and biomolecule concentration detection.

[0014] Furthermore, when performing biomolecule concentration detection, the constructed chiral molecule detection sensor is immersed in chiral solutions with different concentration gradients, and the optical detection system is used to introduce left-handed and right-handed circularly polarized light to irradiate and collect scattering signals, thereby obtaining the corresponding circular differential scattering spectra. There is a one-to-one correspondence between different concentrations of chiral molecules and the peak shift of the circular differential scattering spectrum. By analyzing the peak position changes in the spectrum, a quantitative relationship between the chiral molecule concentration and the peak shift is established, thereby realizing the detection of the chiral molecule concentration.

[0015] Compared with the existing technology, the beneficial effects of the present invention are as follows: the present invention provides a method for identifying the configuration of chiral molecules, which utilizes the coupling of the local chiral near-field of chiral metal nanoparticles with the surface Bragg mode of the reflector substrate to form a narrow CDS peak. By detecting the relative size of the CDS peak shift, the chiral configuration and concentration of the chiral molecule can be determined. Compared with the existing technology, the chiral molecule sensing platform has high sensitivity, short detection time, no labeling, no washing, and no need for chiral antibodies. It is also low-cost, can be prepared on a large scale, and has strong scalability, and has extremely high market value and application prospects. In addition, the present invention breaks the limitation that a single nanostructure is only adapted to specific molecules, and is suitable for applications in clinical medicine and disease diagnosis, environmental protection and pesticide residue monitoring, and biomolecule concentration detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Flowchart of the method for identifying the configuration of a chiral molecule provided in Example 1 of the present application; in the figure: 1. Silica medium; 2. Tantalum pentoxide medium; 3. Gold nanoparticles; 4. Phenylalanine aqueous solution.

[0017] Figure 2 A scanning electron microscope image of a cross section of a distributed Bragg reflector provided in Example 1 of the present application; Figure 3This is a scanning electron microscope image of left-handed and right-handed gold nanoparticles dispersed on a SiO2 / Si substrate provided in Example 1 of the present application; Figure 4 Dark field optical imaging of chiral nanoparticles on a DBR substrate provided in Example 1 of the present application; Figure 5 Circular differential scattering spectrum of the chiral nanoparticles provided in Example 1 of the present application on a silica substrate; Figure 6 Circular dichroism spectrum of the chiral nanoparticles provided in Example 1 of the present application in aqueous solution; Figure 7 Circular difference scattering spectrum of the chiral nanoparticles provided in Example 1 of the present application on a silica substrate and immersed in a 1 μM L- / D-phenylalanine molecular solution; Figure 8 The circular difference scattering spectrum of the achiral gold nanocube provided in Example 1 of the present application on a DBR substrate and immersed in a 1 μM L- / D-phenylalanine molecular solution; Figure 9 Circular difference scattering spectrum of the achiral spherical nanoparticles provided in Example 1 of the present application on a DBR substrate and immersed in a 1 μM L- / D-phenylalanine molecular solution; Figure 10 Circular difference scattering spectrum of the left-handed nanoparticles provided in Example 1 of the present application on a DBR substrate and immersed in a 1 μM L- / D-phenylalanine molecular solution; Figure 11 Circular difference scattering spectrum of the right-handed nanoparticles provided in Example 1 of the present application on a DBR substrate and immersed in a solution of L- / D-phenylalanine molecules; Figure 12 Circular difference scattering spectra of left-handed nanoparticles provided in Example 2 of the present application and chiral molecule detection of L-phenylalanine molecules at different concentrations; Figure 13 Circular difference scattering spectra of chiral molecule detection of right-handed nanoparticles and L-phenylalanine molecules at different concentrations provided in Example 2 of the present application; Figure 14 Circular difference scattering spectra of left-handed nanoparticles and chiral molecule detection of D-phenylalanine molecules at different concentrations provided in Example 2 of the present application; Figure 15 Circular difference scattering spectra of chiral molecule detection of right-handed nanoparticles and D-phenylalanine molecules at different concentrations provided in Example 2 of the present application; Figure 16The chiral molecular configuration recognition device provided in Example 2 of the present application realizes the chiral molecule configuration recognition in 10 -4 M to 10 -14 Detection results within the M concentration range. DETAILED DESCRIPTION

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0019] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0020] The present invention will be further described below with reference to specific examples, but they are not intended to limit the present invention.

[0021] like Figure 1 As shown, an embodiment of the present invention provides a method for identifying the configuration of a chiral molecule, comprising the following steps: First, two or more materials with different refractive indices are alternately deposited on a substrate to form a reflector substrate with a periodic multilayer film structure; In this step, when different refractive index materials are periodically deposited on the substrate, one of the materials between adjacent film layers is a high refractive index material and the other is a low refractive index material, wherein the high refractive index material in the reflector substrate is 、ZnS、 or One or more of the following, the low refractive index material is a polymer, 、 or In order to ensure a moderate reflectivity at the interface to form effective Bragg interference and avoid excessive interface stress leading to film peeling, the refractive index difference between the two materials of adjacent layers is 0.2-1. In order to strike a balance between obtaining sufficient reflectivity and controlling costs, and based on the phase matching principle, the optical path difference of the reflected light from adjacent interfaces is / 2 to achieve maximum reflection efficiency, the number of film layers on the reflector substrate is 5-20 layers, and the thickness of each film is / ,in, is the central wavelength, is the refractive index of the film material. In the actual fabrication process, the reflection wavelength can be adjusted by adjusting the thickness of the periodic film layer. The reflective spectrum of the reflector substrate with a periodic multilayer film structure fabricated using the above method has a central wavelength range of 400 nm to 1000 nm.

[0022] Depositing metal chiral nanoparticles on a reflector substrate to obtain a chiral molecule detection sensor, and immersing the chiral molecule detection sensor in a solution of chiral molecules to be detected; In this step, chiral metal nanoparticles are first obtained or prepared, wherein the chiral metal nanoparticles are one or more of gold, silver, or copper-based chiral nanoparticle structures. The chiral metal nanoparticles to be detected are then deposited on a reflector substrate by spin coating or other methods to produce a chiral molecule detection sensor. After deposition, the surface plasmon resonance peaks of the chiral metal nanoparticles to be detected are coupled to the reflector substrate within a wavelength range of 500 nm to 900 nm. The prepared chiral molecule detection sensor is then immersed in a solution of the chiral molecules to be detected.

[0023] Finally, the chiral molecule detection sensor immersed in the chiral molecule solution to be detected is irradiated with left-handed circularly polarized light (LCP) and right-handed circularly polarized light (RCP) to obtain the corresponding circular differential scattering spectra. The spectral peak shift characteristics are then analyzed to determine whether the chirality of the chiral molecule solution to be detected is left-handed or right-handed. In this step, the chirality detection of the chiral molecule solution to be detected relies on the optical detection system and the chiral molecule detection sensor. The optical detection system includes a light source, a linear polarizer, a wave plate, a microscope objective lens, an objective lens, a spectrometer, and a data processing unit arranged in sequence on the optical path. In this embodiment, the light source is a halogen lamp light source. In other embodiments, other light sources that meet the requirements can be selected as needed. The wave plate is / 4 wave plate. In order to generate left-handed circularly polarized light (LCP) and right-handed circularly polarized light (RCP), the angle difference between the polarizer fast axis angle and the wave plate fast axis angle is 90°. Specifically, in this embodiment, the polarizer fast axis angle is +45°, which is used to generate left-handed circularly polarized light (LCP); The angle of the fast axis of the / 4 wave plate is -45°, which is used to generate right-handed circularly polarized light (RCP). The spectrometer is used to collect the scattering spectra under LCP and RCP excitation respectively.

[0024] When performing chiral detection, the halogen lamp light source is polarized by a linear polarizer and / 4 wave plate and then focused by the microscope objective onto the surface of the chiral molecule detection sensor immersed in the chiral molecule solution to be detected. The signal scattered by the chiral molecule detection sensor is collected by the objective lens and sent to the spectrometer. The data processing unit receives the spectral signal scattered by the spectrometer and analyzes and visualizes it to obtain the configuration judgment of the left-handed or right-handed molecules in the chiral molecule solution to be detected.

[0025] In order to better illustrate the present invention, specific examples are given below.

[0026] Example 1 See also Figure 1 As shown, the method for identifying the chiral molecular configuration based on chiral nanoparticles on a distributed Bragg reflector provided in Example 1 specifically includes the following steps: 1) Preparation of distributed Bragg reflector (DBR) substrate The single crystal silicon substrate with high surface flatness after pretreatment was placed in a Veeco ion beam sputtering system, and a high refractive index tantalum pentoxide ( ) and low refractive index silica ( ) alternately constitutes a 12-period dielectric film structure, where and The physical thicknesses are 88 nm and 127 nm respectively. Ion-assisted technology is used in the deposition process to effectively improve the density, optical uniformity and interface quality of the film layer. Finally, a set of high-performance distributed Bragg reflectors is obtained, with a central reflection wavelength of 750 nm, excellent surface flatness and a maximum reflectivity of over 99.95%. Figure 2 As shown, the distributed Bragg reflector has good flatness.

[0027] 2) Construction of chiral molecule detection sensors 100 μL of 10 mM chloroauric acid and 120 μL of 0.10 M ascorbic acid were added to 4.25 mL of deionized water in sequence. After mixing, 10 μL of 0.1 mM L- or D-cysteine ​​solution was added and stirred thoroughly to initiate the reaction. The system was then placed in a 30°C water bath for 2 hours to promote the formation of chiral gold nanoparticles. After the reaction was completed, residual precursors and impurities were removed by centrifugation at 4000 rpm for 5 minutes to obtain a high-purity product. Figure 3 As shown in the figure, scanning electron microscopy images of left-handed and right-handed nanoparticles show that the particles have obvious chiral characteristics and good uniformity. After the precipitate is resuspended and dispersed, it is spin-coated at 4000 rpm for 60 seconds to evenly cover the substrate surface, thereby constructing a molecular detection sensor with chiral optical response function. Figure 4As shown in Figure 2, chiral nanoparticles have a significant scattering effect on the DBR substrate, providing a basis for further study of its optical response. Figure 5 and 6 As shown in , chiral particles have obvious chiral asymmetry on silica substrates or in aqueous solutions, but their peak widths are in the range of 50–100 nm, which severely limits their detection sensitivity, such as Figure 7 As shown in Figure 2, the peak position shift caused by chiral molecules can be ignored. For the achiral gold nanocube (side length of 100 nm) and spherical nanoparticles (particle size of 80 nm), there is no response to the configuration of chiral molecules, as shown in Figure 2. Figure 8 and 9 shown.

[0028] 3) Configuration recognition of chiral molecules The constructed chiral molecular sensor is immersed in a phenylalanine aqueous solution of the chiral configuration to be detected, the concentration of which is 1 μM. The optical detection system is used to introduce left-handed and right-handed circularly polarized light to illuminate it and the microscope objective is used to focus on the surface of the chiral molecule detection sensor immersed in the phenylalanine aqueous solution. The signal scattered by the chiral molecule detection sensor is collected again by the objective lens and sent to the spectrometer, and the scattering response is recorded by the data processing unit to obtain the corresponding circular differential scattering spectrum. By analyzing the spectral peak shift characteristics, the chiral configuration of the molecule can be effectively identified, and the accurate determination of left-handed or right-handed molecules can be achieved. Figure 10 As shown in Figure 2, the left-handed nanoparticles respond more strongly to L-phenylalanine, resulting in a further shift in the peak position, while the peak position shift caused by D-phenylalanine is very weak. Figure 11 As shown in the figure, the results show that D-phenylalanine causes a much larger shift in the circular differential scattering spectrum than L-phenylalanine. Therefore, this chiral molecule detection platform can efficiently identify the molecular configuration.

[0029] This chiral molecular configuration identification method has applications in clinical medicine and disease diagnosis, environmental protection, and pesticide residue monitoring. This method can detect chiral proteins, amino acids, and other biomarkers in biological samples such as blood and urine, and conduct qualitative and quantitative analysis based on the peak shift characteristics of circular differential scattering spectra, enabling early screening and diagnostic monitoring of diseases such as cancer and diabetes. It can also be used to detect environmental pollutants such as chiral pesticide residues and endocrine disruptors in water and soil. By analyzing the spectral peak shifts caused by these pollutants interacting with the sensor, the concentration and chiral configuration of the pollutants can be quickly determined, providing a basis for environmental remediation. For the detection of pesticide residues in agricultural products and chiral substances such as food additives, spectral peak shift analysis can accurately determine the chiral configuration and content of these substances, ensuring food safety and human health. This detection method offers the advantages of rapidity, sensitivity, and high specificity.

[0030] Example 2 This embodiment provides a method for identifying chiral molecular configurations based on chiral nanoparticles on a distributed Bragg reflector. The method can be used for concentration detection of biomolecules and specifically includes the following steps: 1) Preparation of distributed Bragg reflectors The pre-treated single crystal silicon substrate with a highly flat surface is placed in a Veeco ion beam sputtering system, and high refractive index tantalum pentoxide ( ) and low refractive index silica ( ) alternately composed of a multilayer optical structure, totaling 12 periods, with a single layer thickness of 88nm ( ) and 127nm ( Ion-assisted deposition enhances film density, uniformity, and interface quality. The result is a set of high-performance distributed Bragg reflectors (DBRs) with a central reflection wavelength of 750nm, excellent surface smoothness, and a maximum reflectivity exceeding 99.95%.

[0031] 2) Construction of chiral molecule detection sensors To 4.25 mL of deionized water, 100 μL of 10 mM chloroauric acid and 120 μL of 0.10 M ascorbic acid were added sequentially and mixed thoroughly. Then, 10 μL of 0.1 mM aqueous L- or D-cysteine ​​was added and stirred thoroughly to initiate the reaction. The mixture was then placed in a 30°C water bath for 2 hours to induce the self-assembly of chiral gold nanoparticles. After the reaction, unreacted materials and impurities were removed by centrifugation (4000 rpm for 5 minutes) to obtain high-purity nanoparticles. These nanoparticles were resuspended and spin-coated at 4000 rpm for 60 seconds to uniformly deposit them on the surface of the DBR substrate, thus constructing a chiral molecular detection sensor with chiral optical response.

[0032] 3) Concentration detection of chiral molecules The constructed chiral molecule detection sensor was immersed in phenylalanine aqueous solution with different concentration gradients (concentration range is 、 、 to M), using the optical detection system to introduce left-handed and right-handed circularly polarized light and collect scattered signals to obtain the corresponding circular differential scattering spectrum. Figure 12-15 As shown in the figure, different concentrations of chiral molecules have a one-to-one correspondence with the peak shift of the circular differential scattering spectrum of the system. Through the systematic analysis of the peak position changes in the spectrum, a quantitative relationship between molecular concentration and peak shift can be established, thereby achieving high-sensitivity detection of chiral molecule concentration. Figure 16 As shown in the figure, the peak position shift difference of a single chiral molecule on left-handed and right-handed chiral particles is defined as , this difference has a linear functional relationship with the concentration of phenylalanine, and its detection limit is M.

[0033] The above are only preferred embodiments of the present invention and do not limit the implementation mode and protection scope of the present invention. For those skilled in the art, it should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the contents of the present invention specification should be included in the protection scope of the present invention.

Claims

1. A method for identifying the configuration of a chiral molecule, characterized in that: The steps include: A reflector substrate having a periodic multilayer film structure is formed by alternately depositing two or more materials with different refractive indices on a substrate; Depositing metal chiral nanoparticles on a reflector substrate to obtain a chiral molecule detection sensor, and immersing the chiral molecule detection sensor in a solution of chiral molecules to be detected; The chiral molecule detection sensor immersed in the chiral molecule solution to be detected is irradiated with left-handed circularly polarized light (LCP) and right-handed circularly polarized light (RCP), and the corresponding circular differential scattering spectra are obtained. The spectral peak shift characteristics are analyzed to determine the left-handed or right-handed configuration of the chiral molecule solution to be detected.

2. The method for identifying the configuration of a chiral molecule according to claim 1, wherein: In step 1, the refractive index difference between the two materials of adjacent film layers is 0.2-1.

3. The method for identifying the configuration of a chiral molecule according to claim 2, wherein: The high refractive index material in the reflector substrate is 、ZnS、 or One or more of the following, the low refractive index material is a polymer, 、 or One or more of .

4. The method for identifying the configuration of a chiral molecule according to claim 1, wherein: In step 1, the number of film layers of the reflector substrate is 5-20, and the thickness of each film layer is / ,in, is the central wavelength, is the refractive index of the film material.

5. The method for identifying the configuration of a chiral molecule according to claim 1, wherein: In step 2, the metal chiral nanoparticles are one or more chiral nanoparticle structures based on gold, silver or copper.

6. A device for implementing the method for identifying the configuration of a chiral molecule according to any one of claims 1 to 5, characterized in that: It includes a chiral molecule detection sensor and an optical detection system, wherein: A chiral molecule detection sensor comprises a reflector substrate with a periodic multilayer film structure and metal chiral nanoparticles deposited on the reflector substrate, wherein the refractive indices of adjacent film layers on the reflector substrate are different; An optical detection system comprising a light source, a linear polarizer, a wave plate, a microscope objective lens, an objective lens, a spectrometer, and a data processing unit sequentially arranged on an optical path; When performing chirality detection, the chiral molecule detection sensor is immersed in the chiral molecule solution to be detected. The light generated by the light source is focused by the microscope objective lens onto the surface of the chiral molecule detection sensor in the solution after passing through the linear polarizer and wave plate. The signal scattered by the chiral molecule detection sensor is collected by the objective lens and sent to the spectrometer. The data processing unit receives the spectral signal scattered by the spectrometer and processes it to obtain the chiral configuration of the chiral molecule solution to be detected.

7. The device for identifying chiral molecular configuration according to claim 6, characterized in that: The central wavelength range of the reflection spectrum of the reflector substrate is 400-1000 nm, and the coupling wavelength range of the surface plasmon resonance peak of the metal chiral nanoparticles and the reflector substrate is 500-900 nm.

8. The device for identifying chiral molecular configuration according to claim 6, characterized in that: The angle difference between the fast axis angle of the polarizer and the fast axis angle of the wave plate is 90°, which is used to generate left-handed circularly polarized light LCP and right-handed circularly polarized light RCP respectively.

9. Use of a device for identifying chiral molecular configuration according to any one of claims 6 to 8, characterized in that: The device is used in clinical medicine and disease diagnosis, environmental protection, pesticide residue monitoring, and biomolecule concentration detection.

10. Use of the device for identifying chiral molecular configuration according to claim 9, characterized in that: When performing biomolecule concentration detection, the constructed chiral molecule detection sensor is immersed in chiral solutions with different concentration gradients. The optical detection system is used to introduce left-handed and right-handed circularly polarized light and collect the scattered signals to obtain the corresponding circular differential scattering spectra. There is a one-to-one correspondence between different concentrations of chiral molecules and the peak shift of the circular differential scattering spectrum. By analyzing the peak position changes in the spectrum, a quantitative relationship between the chiral molecule concentration and the peak shift is established, thereby realizing the detection of the chiral molecule concentration.

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