A plant extract with immediate and long-lasting whitening, soothing, and anti-inflammatory effects, its preparation method, and its application.

Abstract

This invention belongs to the field of cosmetic technology and discloses a plant extract with immediate and long-lasting whitening, soothing, and anti-inflammatory effects, as well as its preparation method and application. The plant extract includes licorice extract, tea extract, scutellaria root extract, and mulberry root bark extract. Using licorice, tea, scutellaria root, and mulberry root bark in a mass ratio of 1-5:1-5:1-3:1-3 as raw materials, the extract is obtained by compounding multiple plant extracts and using a semi-bionic method with an assisted eutectic solvent for extraction. This allows the components to exert a synergistic effect, achieving whitening and skin care while also providing highly effective soothing and anti-inflammatory effects. It is fast-acting, has strong immediate effects, and provides long-lasting whitening. It also has certain acne-reducing and oil-controlling effects, offering diverse efficacy. The ingredients are safe, reliable, and effective, and can be applied to the preparation of skin care products or skin preparations. It is very suitable for people aged 18-30.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a plant extract with immediate and long-lasting whitening, soothing and anti-inflammatory effects, its preparation method and application. Background Technology

[0002] After years of development, skin-whitening cosmetics have evolved from simple physical concealing and whitening to exfoliating the stratum corneum, and then to synergistic whitening through the action of various whitening ingredients. Each stage of development represents a technological innovation, and the current trend is towards healthy whitening. With in-depth research into skin-whitening cosmetics, consumers have new demands: they value natural beauty rather than simply whitening. They want to whiten their skin while maintaining its health, and to nourish it while pursuing whitening. In recent years, more and more skin-whitening agents have appeared on the market, such as kojic acid, arbutin, and azelaic acid, which are widely used in skin-whitening cosmetics as tyrosinase inhibitors. However, these whitening agents mostly act on a single pathway, and the whitening effect is often not obvious. In addition, these whitening agents also have certain safety risks. For example, arbutin and kojic acid may cause contact dermatitis, and azelaic acid can irritate the skin and cause temporary erythema.

[0003] Skin irritation refers to a reaction of the skin when exposed to irritating substances, mainly manifested as symptoms such as redness, swelling, stinging, and itching. In recent years, the diversification of cosmetics and cosmetic raw materials has increased the possibility of inducing skin irritation; moreover, people are paying more and more attention to skin care, and their understanding of skin irritation is gradually deepening, making cosmetics and cosmetic raw materials claiming soothing and anti-inflammatory effects increasingly popular.

[0004] Currently, the market offers a wide variety of products with different effects. Consumers, facing multiple facial concerns, often need to use multiple products in combination to overcome the limitations of single-function products. This leads to a multitude of products, complex usage, time-consuming and laborious processes, slow results, and the risk of ingredient incompatibility between products, potentially damaging the skin. The skin of the 18-30 age group is characterized by low melanin content, low skin tone, and minimal wrinkles and age spots. However, due to their fast-paced lifestyles and high stress levels, this group is prone to various skin problems, resulting in a more complex skin condition. Consequently, their skincare needs are rapidly increasing, and their demands for product efficacy are rising. They prioritize gentleness, immediate results, and long-lasting effects, making diversified skincare a key characteristic of this age group. Therefore, given the diverse efficacy needs of the 18-30 age group, developing a whitening product with soothing and anti-inflammatory properties is essential. Summary of the Invention

[0005] In order to overcome the shortcomings and deficiencies of the existing technology, the primary objective of this invention is to provide a method for preparing a plant extract with immediate and long-lasting whitening, soothing and anti-inflammatory effects.

[0006] Another objective of this invention is to provide a plant extract prepared by the above-mentioned preparation method that has immediate and long-lasting whitening, soothing, and anti-inflammatory effects.

[0007] Another object of the present invention is to provide the application of the above-mentioned plant extracts with immediate and long-lasting whitening and soothing anti-inflammatory effects.

[0008] The objective of this invention is achieved through the following technical solution:

[0009] A method for preparing a plant extract with immediate and long-lasting whitening, soothing, and anti-inflammatory effects includes the following steps:

[0010] (1) Dry the licorice, tea, Scutellaria baicalensis root and mulberry root bark separately, pulverize and sieve them, weigh the pulverized and sieved licorice, tea, Scutellaria baicalensis root and mulberry root bark powders in a mass ratio of 1-5:1-5:1-3:1-3, mix them evenly to obtain mixed powder;

[0011] (2) Weigh out choline chloride, citric acid and ethylene glycol in a molar ratio of 3:2:1 and mix them. Use a vacuum rotary evaporator to heat the mixture at 70-80℃ for 2-4 hours to dissolve it into a uniform liquid solvent. Cool and filter the resulting liquid solvent to obtain a pure DES system.

[0012] (3) Extraction was performed using a semi-bionic method with the aid of a eutectic solvent. Specifically, the following steps were followed: The mixed powder obtained in step (1) was extracted by reflux with a solution of disodium hydrogen phosphate-citric acid buffer at pH 2.2, 7.4 and 8.0 at a ratio of 1g:15-25mL. Each reflux extraction was performed for 25-35 min, followed by filtration. The filtrate obtained from the three filtrations was retained. The final filter residue was then mixed with the DES system obtained in step (2) at a ratio of 1g:20-35mL. The mixture was extracted at 45-55℃ for 1-3 h and then filtered. The resulting filtrate was mixed with the filtrate obtained from the three filtrations to obtain the DES extract.

[0013] (4) The DES extract obtained in step (3) is added to ethylene oxide-propylene oxide copolymer (EOPO) at a mass ratio of 3:2 and shaken to mix. After centrifugation at 4500-5500 r / min, an upper EOPO enriched phase and a lower DES enriched phase are obtained. At this time, the active ingredient is back-extracted from the DES extract into the EOPO enriched phase. After separation, the EOPO enriched phase is placed in a water bath at 60-70℃ for 45-75 min for temperature-induced phase separation. After centrifugation at 4500-5500 r / min, an EOPO phase and an aqueous phase are obtained. At this time, the active ingredient is enriched in the aqueous phase. Then, the aqueous phase is centrifuged at 4500-5500 r / min and filtered with a 0.45-5µm filter membrane. The filtrate is concentrated to a paste by vacuum distillation to obtain a mixture.

[0014] (5) Spray dry or bake dry the mixture obtained in step (4), mix and pulverize, and sieve to obtain a plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects.

[0015] The drying in step (1) is carried out overnight in a forced-air drying oven at 45-55℃; the pulverization and sieving is carried out through an 80-mesh sieve.

[0016] The sieving in step (5) is sieving through an 80-mesh sieve.

[0017] A plant extract prepared by the above method, which has immediate and long-lasting whitening, soothing and anti-inflammatory effects.

[0018] The plant extract contains akebia phenylethanol glycoside B, isoliquiritigenin, L-theanine, and 5,7-dihydroxycoumarin.

[0019] The above-mentioned plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects is used in the preparation of whitening skin care products with high efficacy in soothing and anti-inflammatory effects.

[0020] A soothing and anti-inflammatory whitening composition comprising the above-mentioned plant extracts, short-chain alcohols and deionized water.

[0021] The preparation method of the above-mentioned soothing and anti-inflammatory whitening composition includes the following steps: stirring the above-mentioned plant extract, short-chain alcohol and deionized water at a speed of 300-350 r / min for 10-15 min to obtain the soothing and anti-inflammatory whitening composition; wherein the short-chain alcohol is at least one of 1,3-butanediol, 1,3-propanediol and 1,2-hexanediol.

[0022] Preferably, the mass ratio of the plant extract, short-chain alcohol and deionized water is (1~5):(15~40):(5~45).

[0023] Preferably, the short-chain alcohol is 1,3-butanediol and / or 1,2-hexanediol.

[0024] Preferably, the short-chain alcohol is 1,3-butanediol and 1,2-hexanediol.

[0025] Preferably, the mass ratio of 1,3-butanediol to 1,2-hexanediol is (1-35):(1-5).

[0026] Preferably, the mass ratio of 1,3-butanediol to 1,2-hexanediol is 7:1.

[0027] The above-mentioned soothing and anti-inflammatory whitening composition is used in the preparation of an essence water cosmetic, wherein the essence water cosmetic is prepared according to the following steps:

[0028] (1) Weigh water, glycerol, disodium EDTA and p-hydroxyacetophenone, mix them evenly, heat to 85-90℃, homogenize for 1-4 min, keep warm for 20-25 min to obtain phase A;

[0029] (2) After the A phase is kept warm, wait for the temperature to drop to 45-50℃, add the whitening composition, stir evenly, filter with 400 mesh filter cloth to obtain essence water cosmetic.

[0030] The principle of this invention:

[0031] 1. In this patent application, the combined use of four plant extracts—licorice extract, scutellaria root extract, tea extract, and mulberry root bark extract—not only exhibits a synergistic effect but also possesses certain combined advantages. Licorice extract, scutellaria root extract, tea extract, and mulberry root bark extract all contain phenolic hydroxyl structures, giving them extremely strong antioxidant and antibacterial effects. They can effectively scavenge free radicals, reduce pigmentation formation, and resist inflammatory damage to the skin caused by external bacteria. While achieving highly effective whitening by scavenging free radicals and fading pigmentation, they also soothe and reduce inflammation in the skin, synergistically repairing skin problems instantly. This invention combines four extracts with short-chain alcohols and deionized water to create a whitening composition that effectively improves the stability of the composition system and achieves a long-lasting whitening effect. This is because the polarity of the active ingredients in the compound plant extracts allows them to form a stable encapsulation phase with the short-chain alcohols. The active molecules in the whitening composition maintain each other due to the aqueous system they form, thus avoiding impurity reactions caused by collisions between active molecules in the aqueous system. This results in excellent self-stability of the composition, thereby achieving a long-lasting release effect.

[0032] 2. In this patent application, the combination of the above-mentioned plant extracts can effectively enhance the whitening effect of the composition and its application products, and can also effectively soothe and reduce inflammation, as well as have a certain effect on acne removal and oil control. This is mainly because the flavonoids in licorice extract can inhibit the activity of tyrosinase and dopachrome tautomerase (TRP-2), thereby preventing the formation of melanin and achieving the effect of whitening the skin. It can also inhibit the synthesis and release of inflammatory cytokines and inflammatory mediators, inhibit the activation of IκB kinase (IKK), and prevent the transcription of nuclear factor-κB (NF-κB), thus playing an anti-inflammatory role. The glycosides in Scutellaria baicalensis root extract have high inhibitory activity against melanocytes and have no toxic side effects on other human cells such as basal cells. While whitening the skin, they can protect the skin from damage. Glycosides also have significant anti-inflammatory activity, such as anti-passive skin allergy. Theanine in tea extract is a unique amino acid of tea, which can relieve fatigue and maintain nerve cells. It has multiple benefits, including helping to lower blood pressure. Its fatigue-relieving mechanism is that theanine can activate inhibitory nerves in the brain while inhibiting excitatory nerves, thereby optimizing sleep quality and reducing fatigue symptoms. Further research has confirmed that theanine also has a vasodilatory effect. Meanwhile, the alkaloids in tea extract can effectively remove impurities from the skin, tighten pores, and, in combination with other extracts, significantly enhance the inhibitory effect of inflammatory molecules, effectively activating the skin, soothing skin tension, and promoting skin healing. On the other hand, the coumarins in mulberry root bark extract have various biological activities, such as antioxidant activity (its phenolic hydroxyl structure gives it the ability to scavenge free radicals), anti-inflammatory activity (it can inhibit the production of certain inflammatory mediators), and antibacterial activity (it has a good inhibitory effect on certain bacteria and fungi).

[0033] 3. Demimellar eutectic solvents (DESs) are liquid mixtures formed by mixing hydrogen acceptors (such as choline chloride) and hydrogen donors (such as sugars, alcohols, or carboxylic acids) in a suitable ratio. They are economical, non-toxic, recyclable, biodegradable, and environmentally friendly, exhibiting good solubility and extractability for organic compounds. As environmentally friendly "green" solvents, they have the potential to replace organic solvents as extraction agents. Currently, common methods for extracting active ingredients include organic solvent extraction, ultrasound-assisted extraction, supercritical fluid extraction, and microwave-assisted extraction. However, these methods typically use large amounts of organic solvents, posing safety hazards such as high solvent consumption, high energy consumption, environmental pollution, and flammability / explosiveness. Furthermore, due to the structural differences between glycyrrhizic acid, a large amount found in licorice root, and other active ingredients, traditional extraction methods cannot simultaneously extract and separate it from other hydrophilic active ingredients.

[0034] Semi-bionic extraction, from a biopharmaceutics perspective, combines holistic drug research with molecular drug research, simulating the gastrointestinal transport and absorption environment of orally administered drugs. It employs an activity-guided directed separation method. Based on the drug's absorption process in vivo, solvents with different pH values ​​are used for sequential extraction to obtain the most comprehensive range of active ingredients from the raw materials. The extraction process aligns with the characteristics of traditional Chinese medicine formulation and clinical drug use, as well as the gastrointestinal transport and absorption characteristics of orally administered drugs. It fully leverages the synergistic effects of the mixture, considering both active mixed components and individual component indicators, which is beneficial for controlling the quality of traditional Chinese medicine preparations while minimizing the loss of active ingredients.

[0035] In this patent application, a semi-biomimetic method is employed to assist in eutectic solvent extraction. First, a preliminary extraction is performed under acidic conditions using a semi-biomimetic method, causing some flavonoids and polyphenols to dissolve from the cells. Then, a eutectic solvent is used for further extraction. The properties of the eutectic solvent allow for more complete extraction of the remaining flavonoids and polyphenols. Furthermore, the extraction rate and purity of flavonoids and polyphenols can be improved by adjusting the composition of the eutectic solvent and the extraction conditions. This approach saves resources, time, and effort, while also improving extraction efficiency and ensuring the purity and active content of the plant extract. Specifically, tea polyphenols in the tea extract can combine with macromolecular chain free radicals through their active hydrogen atoms to form stable free radical groups. This further increases the steric hindrance in the system, enhancing the protection of free radical groups, thereby achieving site aggregation of stable compounds in the system, reducing the intervention of impurity molecules, and thus retaining more active ingredients, maintaining the efficacy of the composition, and increasing the composition's inherent stability.

[0036] The present invention has the following advantages and beneficial effects compared with the prior art:

[0037] This invention combines multiple plant extracts and uses a semi-biomimetic method with an eutectic solvent for extraction, enabling the components to work synergistically. This results in skin whitening and skincare with highly effective soothing and anti-inflammatory effects. It is fast-acting, provides immediate relief, and offers long-lasting whitening. It also has some acne-reducing and oil-controlling effects. With its diverse efficacy and safe, reliable, and effective ingredients, this product can be used in the preparation of skincare products or skin formulations and is very suitable for people aged 18-30. Attached Figure Description

[0038] Figure 1 This is the total ion chromatogram of the extract.

[0039] Figure 2 This is a fragment ion diagram of Akebia trifoliata phenylethanoid B.

[0040] Figure 3 This is an ion diagram of isoliquiritigenin fragments.

[0041] Figure 4 This is a fragment ion diagram of L-theanine.

[0042] Figure 5 This is a fragment ion diagram of 5,7-dihydroxycoumarin.

[0043] Figure 6 This is a graph showing the effects of negative control, positive control, and different group concentrations on IL-6 expression.

[0044] Figure 7 This is a graph showing the effects of negative control, positive control, and different group concentrations on PGE2 expression. Detailed Implementation

[0045] The following specific embodiments further illustrate the content of the present invention, but should not be construed as limiting the present invention.

[0046] Example 1

[0047] (1) Place licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark in a 50℃ forced-air drying oven and dry overnight. Grind them through an 80-mesh sieve. Weigh the powders of licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark obtained by grinding and sieving according to a mass ratio of 5:2:2:1, mix them evenly, and obtain a mixed powder.

[0048] (2) Weigh out choline chloride, citric acid and ethylene glycol in a molar ratio of 3:2:1 and mix them in a round-bottom flask. Use a vacuum rotary evaporator to heat the mixture at 75°C for 3 h to dissolve it into a uniform liquid solvent and obtain a colorless and transparent liquid. Cool and filter the obtained liquid solvent to obtain a pure DES system.

[0049] (3) Extraction was performed using a semi-bionic method with the aid of a eutectic solvent. Specifically, the following steps were followed: The mixed powder obtained in step (1) was extracted by reflux with disodium hydrogen phosphate-citric acid buffer solution with pH values ​​of 2.2, 7.4 and 8.0 at a ratio of 1 g: 18 mL. After each reflux extraction for 30 min, the mixture was filtered and the filtrate obtained from the three filtrations was retained. Then, the final filter residue was mixed with the DES system obtained in step (2) at a ratio of 1 g: 25 mL. The mixture was extracted at 50 °C for 2 h and then filtered. The resulting filtrate was mixed with the filtrate obtained from the three filtrations to obtain the DES extract.

[0050] (4) The DES extract obtained in step (3) was added to ethylene oxide-propylene oxide copolymer (EOPO) at a mass ratio of 3:2 and mixed by shaking. After centrifugation at 5000 r / min, an upper EOPO enriched phase and a lower DES enriched phase were obtained. At this time, the active ingredient was back-extracted from the DES extract into the EOPO enriched phase. After separation, the EOPO enriched phase was placed in a 65℃ water bath for 60 min for temperature-induced phase separation. After centrifugation at 5000 r / min, the EOPO phase and the aqueous phase were obtained. At this time, the active ingredient was enriched in the aqueous phase. Then, the aqueous phase was centrifuged at 5000 r / min and filtered with a 0.45µm filter membrane. The filtrate was concentrated to a paste by vacuum distillation to obtain a mixture.

[0051] (5) Spray dry or bake dry the mixture obtained in step (4), mix and pulverize it, and pass it through an 80-mesh sieve to obtain a plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects.

[0052] Qualitative analysis of the active ingredients in the obtained plant extracts was performed using MS in negative ion mode. The total ion map and fragment ion maps of some active substances are shown below. Figure 1-5 As shown, the extract contains akebia phenylethanol glycoside B, isoliquiritigenin, L-theanine and 5,7-dihydroxycoumarin.

[0053] Example 2

[0054] (1) Place licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark in a 50℃ forced-air drying oven and dry overnight. Grind them and pass them through an 80-mesh sieve. Weigh the powders of licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark obtained by grinding and sieving according to a mass ratio of 2:5:2:1, mix them evenly, and obtain a mixed powder.

[0055] (2) Weigh out choline chloride, citric acid and ethylene glycol in a molar ratio of 3:2:1 and mix them in a round-bottom flask. Use a vacuum rotary evaporator to heat the mixture at 75°C for 3 h to dissolve it into a uniform liquid solvent and obtain a colorless and transparent liquid. Cool and filter the obtained liquid solvent to obtain a pure DES system.

[0056] (3) Extraction was performed using a semi-bionic method with the aid of a eutectic solvent. Specifically, the following steps were followed: The mixed powder obtained in step (1) was extracted by reflux with disodium hydrogen phosphate-citric acid buffer solution with pH values ​​of 2.2, 7.4 and 8.0 at a ratio of 1 g: 15 mL. After each reflux extraction for 30 min, the mixture was filtered and the filtrate obtained from the three filtrations was retained. Then, the final filter residue was mixed with the DES system obtained in step (2) at a ratio of 1 g: 35 mL. The mixture was extracted at 50 °C for 2 h and then filtered. The resulting filtrate was mixed with the filtrate obtained from the three filtrations to obtain the DES extract.

[0057] (4) The DES extract obtained in step (3) was added to ethylene oxide-propylene oxide copolymer (EOPO) at a mass ratio of 3:2 and mixed by shaking. After centrifugation at 5000 r / min, an upper EOPO enriched phase and a lower DES enriched phase were obtained. At this time, the active ingredient was back-extracted from the DES extract into the EOPO enriched phase. After separation, the EOPO enriched phase was placed in a 65℃ water bath for 60 min for temperature-induced phase separation. After centrifugation at 5000 r / min, the EOPO phase and the aqueous phase were obtained. At this time, the active ingredient was enriched in the aqueous phase. Then, the aqueous phase was centrifuged at 5000 r / min and filtered with a 0.45µm filter membrane. The filtrate was concentrated to a paste by vacuum distillation to obtain a mixture.

[0058] (5) Spray dry or bake dry the mixture obtained in step (4), mix and pulverize it, and pass it through an 80-mesh sieve to obtain a plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects.

[0059] Example 3

[0060] (1) Place licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark in a 50℃ hot air drying oven and dry overnight. Grind them through an 80-mesh sieve. Weigh the powders of licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark obtained by grinding and sieving according to a mass ratio of 5:3:1:1. Mix them evenly to obtain a mixed powder.

[0061] (2) Weigh out choline chloride, citric acid and ethylene glycol in a molar ratio of 3:2:1 and mix them in a round-bottom flask. Use a vacuum rotary evaporator to heat the mixture at 75°C for 3 hours to dissolve it into a uniform liquid solvent and obtain a colorless and transparent liquid. Cool and filter the obtained liquid solvent to obtain a pure DES system.

[0062] (3) Extraction was performed using a semi-bionic method with the aid of a eutectic solvent. Specifically, the following steps were followed: The mixed powder obtained in step (1) was extracted by reflux with disodium hydrogen phosphate-citric acid buffer solution with pH values ​​of 2.2, 7.4 and 8.0 at a ratio of 1 g: 25 mL. After each reflux extraction for 30 min, the mixture was filtered and the filtrate obtained from the three filtrations was retained. Then, the final filter residue was mixed with the DES system obtained in step (2) at a ratio of 1 g: 20 mL. The mixture was extracted at 50 °C for 2 h and then filtered. The resulting filtrate was mixed with the filtrate obtained from the three filtrations to obtain the DES extract.

[0063] (4) The DES extract obtained in step (3) was added to ethylene oxide-propylene oxide copolymer (EOPO) at a mass ratio of 3:2 and mixed by shaking. After centrifugation at 5000 r / min, an upper EOPO enriched phase and a lower DES enriched phase were obtained. At this time, the active ingredient was back-extracted from the DES extract into the EOPO enriched phase. After separation, the EOPO enriched phase was placed in a 65℃ water bath for 60 min for temperature-induced phase separation. After centrifugation at 5000 r / min, the EOPO phase and the aqueous phase were obtained. At this time, the active ingredient was enriched in the aqueous phase. Then, the aqueous phase was centrifuged at 5000 r / min and filtered with a 0.45µm filter membrane. The filtrate was concentrated to a paste by vacuum distillation to obtain a mixture.

[0064] (5) Spray dry or bake dry the mixture obtained in step (4), mix and pulverize it, and pass it through an 80-mesh sieve to obtain a plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects.

[0065] Comparative Example 1

[0066] The specific implementation method of this comparative example is basically the same as that of Example 1, except that the licorice in step (1) is replaced with arborvitae root.

[0067] Comparative Example 2

[0068] The specific implementation method of this comparative example is basically the same as that of Example 1, except that the mass ratio of each raw material powder in step (1) is changed and Centella asiatica is added. The powder of licorice, tea, Scutellaria baicalensis root, mulberry root bark and Centella asiatica in a mass ratio of 4:2:2:1:1 is used.

[0069] Comparative Example 3

[0070] The specific implementation method of this comparative example is basically the same as that of Example 1, except that the mass ratio of each raw material powder in step (1) is changed and licorice is reduced, and powders of tea, Scutellaria baicalensis root and mulberry root bark with a mass ratio of 7:2:1 are used.

[0071] Comparative Example 4

[0072] The specific implementation method of this comparative example is basically the same as that of Example 1, except that the extraction order of step (3) is changed. First, the eutectic solvent is used for extraction and then the semi-bionic extraction is performed. Specifically, the following steps are followed: the mixed powder obtained in step (1) is mixed with the DES system obtained in step (2) at a material-liquid ratio of 1g:20mL, and after extraction at 50℃ for 2h, it is filtered to obtain filtrate 1 and filter residue; the filter residue is refluxed and extracted with disodium hydrogen phosphate-citric acid buffer solution with pH of 2.2, 7.4 and 8.0 in sequence at a material-liquid ratio of 1g:25mL, and filtered after each reflux extraction for 30 min. The filtrates obtained from the three filtrations are combined and then mixed with the aforementioned filtrate 1 to obtain DES extract.

[0073] Comparative Example 5

[0074] Compared with Example 1, the specific implementation method of this comparative example changed the extraction method. The semi-biomimetic method assisted by eutectic solvent extraction was replaced with the traditional organic solvent heating method. The specific operation is as follows:

[0075] (1) Place licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark in a 50℃ hot air drying oven and dry overnight. Grind them through an 80-mesh sieve. Weigh the powders of licorice, tea leaves, Scutellaria baicalensis root and mulberry root bark obtained by grinding and sieving according to a mass ratio of 5:2:2:1. Mix them evenly to obtain a mixed powder.

[0076] (2) The mixed powder obtained in step (1) and the 65% ethanol aqueous solution were mixed at a ratio of 1g:20mL. After stirring evenly, the cell wall was first broken by an ultrasonic cell disruptor for 10min, and then extracted at 85℃ for 2h. After centrifugation, the supernatant was obtained. After vacuum distillation and concentration, the obtained extract was spray-dried or oven-dried, mixed and pulverized, and passed through an 80-mesh sieve to obtain the plant extract.

[0077] Comparative Example 6

[0078] The specific implementation method of this comparative example is the same as steps (1)-(3) of Example 1, except that the extraction method is changed in steps (4)-(5). The active ingredients in the DES extract are recovered by macroporous resin adsorption method. The specific operation steps are as follows:

[0079] (4) Pretreatment of macroporous resin: The macroporous resin was soaked in 95% ethanol at room temperature for 24 h, the supernatant ethanol was discarded, the column was packed by wet method, and the resin column was washed with 95% ethanol until the effluent did not produce white turbidity when water was added; then the resin column was washed with purified water until the effluent had no alcohol odor; then the resin column was washed with 2 BV of 0.03mol / L HCl solution, and then washed with purified water until the effluent was neutral; then the resin column was washed with 2 BV of 1.25mol / L NaOH solution, and finally washed with purified water until the effluent was neutral to obtain the pretreated macroporous resin for use.

[0080] Static adsorption: Accurately weigh the pretreated macroporous resin and the DES extract obtained in step (3) at a ratio of 1 g: 4 mL into an extraction vessel, mix well, and place on a 25 ℃ constant temperature water bath shaker at 110 r·min. -1 The mixture was shaken at a high speed for 4 hours and then left to stand for 12 hours to reach saturation adsorption.

[0081] Static desorption: Take the macroporous resin that has reached static saturation of the adsorption solution, filter it, wash it with water, dry the surface moisture, add 55% ethanol at a volume percentage of 1g:4mL, place it on a 25℃ constant temperature water bath shaker, and desorb at 110r·min. -1 Shake at a high speed for 2 hours, let stand for 8 hours to allow complete desorption, and then collect the eluent for later use.

[0082] (5) Drying: The eluent is concentrated, dried, pulverized, and passed through an 80-mesh sieve to obtain the plant extract.

[0083] Example 1: In vitro antioxidant capacity assay

[0084] DPPH free radical scavenging rate determination

[0085] (1) Experimental principle

[0086] 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable, long-lived free radical. Its ethanol solution is deep purple and exhibits strong absorption around 517 nm. In the presence of free radical scavengers, the light absorption of the DPPH ethanol solution decreases due to the pairing of unpaired electrons with the free radical. The degree of fading of the DPPH ethanol solution is linearly related to the number of electrons it accepts, which can be used to evaluate the ability of a test sample to scavenge free radicals, i.e., the magnitude of its antioxidant activity.

[0087] (2) Experimental steps

[0088] The plant extracts obtained in Examples 1-3 and Comparative Examples 1-6 were dissolved in water to prepare a sample solution with a content of 0.1% (w / w); a 0.2 mmol / L DPPH solution was prepared using ethanol as a solvent; samples were added according to Table 1. After the addition was completed, the mixture was allowed to stand for 30 min. After the reaction was completed, the absorbance was measured at 517 nm.

[0089] Table 1. Sample addition requirements for DPPH free radical scavenging rate determination experiment.

[0090]

[0091] (3) Calculate the DPPH removal rate of the sample according to the following formula.

[0092] Clearance rate (%) = {1 - (T - T0) / (C - C0)} × 100%

[0093] In the formula:

[0094] T is the absorbance of the solution in the sample tube, that is, the absorbance of the solution after the sample reacts with DPPH;

[0095] T0 is the absorbance of the solution in the sample background tube;

[0096] C represents the absorbance of the solution in the DPPH tube, i.e., the absorbance of the DPPH solution without the sample added.

[0097] C0 is the absorbance of the solution in the solvent background tube.

[0098] (4) The results are shown in Table 2

[0099] Table 2 Results of DPPH free radical scavenging rate determination

[0100]

[0101] Table 2 shows that the DPPH free radical scavenging effect of the plant extracts obtained in all examples is greater than that of the plant extracts obtained in the comparative examples. Therefore, the test results indicate that the plant extracts obtained in the embodiments of the present invention can effectively scavenge free radicals and have good antioxidant effects. In contrast, in comparative examples 1-5, replacing or deleting the raw material categories, raw material ratios, extraction order, and extraction methods significantly reduced the DPPH free radical scavenging rate of the obtained plant extracts. This indicates that the present invention, by compounding licorice extract, tea extract, scutellaria root extract, and mulberry root bark extract and using a semi-bionic method assisted by a low-melting-point solvent for extraction, can better improve the DPPH free radical scavenging effect of the obtained plant extracts, which have good antioxidant effects, can maintain and repair somatic cells, enhance the connection between skin cells, and alleviate skin problems such as excessive sebum secretion, increased acne scars, acne, skin redness, and oiliness caused by external stimulation and endocrine disorders. It also helps reduce wrinkles and improve dry skin, making the skin delicate and smooth. As can be seen from Comparative Example 6 after changing the extraction process, using macroporous resin to recover the active ingredients will significantly reduce the scavenging rate of DPPH free radicals by the plant extract. Therefore, only by using the method of the present invention to recover the active ingredients can the active substances be retained to the maximum extent without affecting the DPPH free radical scavenging efficiency, thereby further enhancing the antioxidant effect of the system.

[0102] Example 2: Evaluation of in vitro whitening effect

[0103] The whitening efficacy was evaluated using the in vitro tyrosinase inhibition rate, in accordance with T / GDCA 006—2021.

[0104] (1) Experimental principle

[0105] Melanin synthesis is influenced by tyrosinase, which catalyzes the hydroxylation of L-tyrosine to dopa and then oxidizes dopa to dopaquinone, which forms the final product, melanin. Test substances that inhibit tyrosinase activity can slow down the tyrosinase-catalyzed conversion of L-tyrosine to dopaquinone. The inhibitory effect of the test substance on tyrosinase activity is evaluated by measuring the absorbance of dopaquinone at 475 nm and analyzing the changes in absorbance.

[0106] (2) Experimental steps

[0107] Solution preparation: Accurately prepare a 0.1 mol / L (pH 6.8) phosphate buffer solution and set it as a PBS buffer solution; accurately prepare a 0.5 mg / mL L-tyrosine solution using PBS solution as the solvent; accurately prepare a 500 U / mL tyrosinase solution using PBS solution as the solvent.

[0108] The plant extracts obtained in Examples 1-3 and Comparative Examples 1-6 were dissolved to prepare a 0.1% (w / w) sample solution using deionized water as the solvent. L-tyrosine solution, sample solution, solvent (deionized water), and PBS buffer solution were added sequentially as shown in Table 3. After thorough mixing, the solution was incubated at 37°C for 10 min. Then, the prepared tyrosinase solution was added to each well sequentially, and the mixture was stirred at 37°C for 10 min. After the reaction was completed, four sets of absorbance values ​​were measured at 475 nm immediately.

[0109] Table 3. Sample addition requirements for the tyrosinase inhibition rate determination experiment.

[0110]

[0111] (3) Calculate the tyrosinase inhibition rate according to the following formula.

[0112] Inhibition rate (%) = {1 - (Ad - Ac) / (Ab - Aa)} × 100%

[0113] In the formula:

[0114] Ad is the absorbance of the solution in the sample reaction tube;

[0115] Ac is the absorbance of the solution in the sample background tube;

[0116] Ab represents the average absorbance of the solution in the solvent reaction tube;

[0117] Aa represents the average absorbance of the solution in the solvent background tube.

[0118] (4) The results are shown in Table 4

[0119] Table 4 Results of Tyrosinase Inhibition Rate Measurement

[0120]

[0121] As shown in Table 4, the tyrosinase inhibition effect of the plant extracts obtained in all embodiments is greater than that of the plant extracts obtained in the comparative examples. Therefore, the test results indicate that the plant extracts obtained in the embodiments of the present invention can effectively inhibit tyrosinase activity and have a good whitening effect. In Comparative Examples 1-5, when the types, proportions, extraction order, and extraction methods of raw materials were replaced or deleted, the tyrosinase inhibition rate of the obtained plant extracts decreased significantly. This indicates that the present invention, by combining licorice extract, tea extract, scutellaria root extract, and mulberry root bark extract and using a semi-bionic method assisted by a low-melting solvent for extraction, can significantly inhibit tyrosinase activity, slow down the catalytic conversion of L-tyrosine to dopaquinone by tyrosinase, and prevent excessive melanin formation, thus making the skin brighter and achieving a skin whitening effect. As can be seen from Comparative Example 6 after changing the extraction process, the active substances are significantly reduced when using macroporous resin to recover them. Therefore, the method of the present invention can maximize the retention of active substances without affecting the tyrosinase inhibition rate, thereby further enhancing the whitening effect of the system.

[0122] Example 3: Evaluation of external soothing and anti-inflammatory effects

[0123] When skin is stimulated by external factors, it ultimately produces an irritation response through various pathways. When cells are stimulated, they secrete factors such as IL-6, IL-1α, IL-1β, IL-8, and TNF-α, causing skin irritation. This experiment uses an in vitro model for evaluating soothing and anti-inflammatory effects, employing UVB stimulation of keratinocytes to assess the soothing efficacy. The specific procedures are as follows.

[0124] (1) Cytotoxicology experiment: at 1×10 4Cells were seeded at a density of cells / well into 96-well plates and incubated overnight in an incubator (37°C, 5% CO2). The experiment included a zero-seeding group, a control group, a positive control group, and a sample group. The sample group used plant extracts obtained in Examples 1-3 and Comparative Examples 1-6. Five volume percentage concentration gradients (0.156%, 0.313%, 0.625%, 1.25%, and 2.5%) were set up for each sample in the sample group, with three replicate wells for each concentration gradient. Drug administration was performed when the cell deposition rate in the 96-well plates reached 40%–60%. 200 μL of culture medium containing 10% PBS was added to each well of the control group; 200 μL of culture medium containing 10 wt% DMSO was added to each well of the positive control group; 200 μL of culture medium containing the corresponding concentration of the sample was added to each well of the sample group; the zero-seeding group had no cell seeding, only 200 μL of cell culture medium was added. After drug administration, the 96-well plates were placed in an incubator (37°C, 5% CO2) for incubation. After culturing the cells for 24 h, discard the supernatant, add MTT working solution (0.5 mg / mL), and incubate at 37°C in the dark for 2 h. After incubation, discard the supernatant, add 100 μL of DMSO to each well, and read the A value at 490 nm.

[0125] The formula for calculating relative cell viability is: Relative cell viability = (Sample group A - Zeroing group A) / (Control group A - Zeroing group A) × 100%.

[0126] Cytotoxicity tests showed that when the sample concentration of Examples 1-3 and Comparative Examples 1-6 was 1.25%, the relative cell viability remained at approximately 90%. Furthermore, when the concentration was 0.313% or lower, the plant extracts obtained in Examples 1-3 and Comparative Examples 1-6 exhibited a certain stimulating effect on keratinocyte viability, with relative cell viability gradually increasing as the concentration decreased. However, when the sample concentration was 2.5%, the relative cell viability of the plant extract sample group obtained in Examples 1-3 was approximately 80%, while the relative cell viability of the plant extract sample group obtained in Comparative Examples 1-6 was below 80%. Therefore, to ensure that the relative cell viability remained at a high level, a sample concentration of 0.313% was selected for the following experiments when investigating the effect on the expression of anti-inflammatory factors.

[0127] (2) The soothing test was performed at 1×10 5Cells were seeded at a density of cells / well into 24-well plates and incubated overnight in an incubator (37°C, 5% CO2). The experiment included a blank control group (BC), a negative control group (NC), a positive control group (PC), and a sample group. The sample group was prepared with a concentration of 0.313% (V / V). Drug administration was initiated when the cell deposition rate in the 24-well plates reached 40%–60%. 1 mL of cell culture medium was added to each well of the blank control group and negative control group; 1 mL of culture medium containing 100 μg / mL vitamin C and 7 μg / mL vitamin E was added to each well of the positive control group; and 1 mL of culture medium containing the corresponding concentration of the test substance (i.e., the plant extracts obtained in Examples 1-3 and Comparative Examples 1-6) was added to each well of the sample group. Twenty-four hours after drug administration, the negative control group (NC), the positive control group (PC), and the sample group received a total dose of 300 mJ / cm². 2 The UVB radiation was applied to the control group BC, which was placed in the same environment (UVB radiation dose was 0 mJ / cm²). 2 After 24 hours of incubation, the cell culture supernatant was collected into EP tubes and stored at -80°C. IL-6 levels were measured according to the instructions of the Human IL-6 ELISA kit. PGE2 levels were measured according to the instructions of the Prostaglandin E2 ELISA kit.

[0128] (3) Experimental conclusions

[0129] Effects of different treatment groups on the expression of anti-inflammatory factors in cells

[0130] From Table 5 and Figure 6 It can be seen that, compared with the BC group, the secretion of the keratinocyte inflammatory factor IL-6 in the NC group was significantly increased, indicating that the stimulation conditions in this experiment were effective. Compared with the NC group, the PC group could significantly reduce the secretion of the keratinocyte inflammatory factor IL-6, indicating that the positive control in this experiment was effective. Compared with the NC group, the influence of different groups on IL-6 expression was greater in all the plant extracts obtained in the examples than in the plant extracts obtained in the comparative example. That is, the plant extracts obtained in Examples 1-3 could significantly reduce the secretion of the keratinocyte inflammatory factor IL-6, while the inhibitory effect of the plant extracts obtained in the comparative example was slightly lower than that of the examples.

[0131] Table 5. Effects of blank control group, negative control group, positive control group, and different sample groups on IL-6 expression.

[0132]

[0133] From Table 6 and Figure 7It was found that, compared with the BC group, the secretion of the keratinocyte inflammatory factor PGE2 in the NC group was significantly increased (p<0.05), indicating that the stimulation conditions in this experiment were effective. Compared with the NC group, the PC group could significantly reduce the secretion of the keratinocyte inflammatory factor PGE2 (p<0.01), indicating that the positive control in this experiment was effective. Compared with the NC group, the influence of different groups on PGE2 expression was greater in all the plant extracts obtained in the examples than in the comparative example. That is, the plant extracts obtained in Examples 1-3 could significantly reduce the secretion of the keratinocyte inflammatory factor PGE2, while the inhibitory effect of the plant extracts obtained in the comparative example was slightly lower than that in the examples.

[0134] Table 6. Effects of blank control group, negative control group, positive control group and different sample groups on PGE2 expression.

[0135]

[0136] Under the test conditions, based on the UVB-stimulated keratinocyte (HaCaT) model, the plant extract of this invention significantly reduced the secretion of keratinocyte inflammatory factors IL-6 and PGE2 at a dosage concentration of 0.313%, indicating that the plant extract of this invention has a soothing and anti-inflammatory effect on the skin within a certain dosage range. This is because the baicalin contained therein reduces the release of chemical mediators during antibody-antigen reactions by inhibiting the activity of sulfhydryl enzymes, thereby inhibiting inflammatory factors and exerting an anti-inflammatory effect. The alkaloids in the tea extract can effectively remove impurities from the skin, tighten pores, and work together with other combined extracts to significantly enhance the inhibitory effect of inflammatory molecules, effectively activating the skin, soothing skin tension, and promoting skin healing. Licorice root extract can reduce melanin deposition caused by inflammation, accelerate keratin renewal, and help the skin regain its radiance.

[0137] Example 4: Immediate and Long-Lasting Whitening Effect Test

[0138] (1) Preparation of instant and long-lasting whitening essence water

[0139] The plant extracts obtained in Examples 1-3 and Comparative Examples 1-6 were first prepared into a whitening composition according to the following steps: the plant extracts, short-chain alcohols (including 1,3-butanediol and 1,2-hexanediol in a mass ratio of 7:1) and deionized water were prepared in a mass ratio of 2:28:30, and then stirred at 350 r / min for 10 min to obtain a soothing and anti-inflammatory whitening composition.

[0140] The whitening composition obtained above is then prepared into an essence water according to the following steps:

[0141] a. Weigh 80g of water, 15g of glycerol, 0.04g of disodium EDTA and 0.5g of p-hydroxyacetophenone, mix them evenly, put them into the main pot, heat to 85℃, homogenize for 4min, keep warm for 20min, and obtain phase A.

[0142] b. Weigh 2g of the whitening composition and set aside for phase B;

[0143] c. After the A phase has finished its heat preservation process, wait for the temperature to drop to 45℃, then add the weighed B phase, add water until the total weight is 100g, and then stir evenly; this yields the essence water cosmetic.

[0144] d. After passing the inspection, filter the material through a 400-mesh filter cloth.

[0145] (2) Test method

[0146] Instant whitening effect test:

[0147] Referring to the testing method for the skin whitening and blemish-removing efficacy of cosmetics (Method 2), 66 healthy volunteers aged 18-30 years were selected and randomly divided into 11 groups of 6 people each. They applied essence water made from plant extracts obtained in Examples 1-3, essence water made from plant extracts obtained in Comparative Examples 1-6, and two commercially available skin whitening and blemish-removing products (where the main active ingredient of product A is glycyrrhizic acid, and the main active ingredient of product B is tea polyphenols) to their faces. Facial color parameters were measured using a skin color meter (Colormeter CL400; CK, Germany) at four time points: before use and 10 minutes, 30 minutes, and 2 hours after use. The skin translucency (ITA°) of the volunteers was obtained, and the results are shown in Table 7. The calculated values ​​are averages.

[0148] The improvement rate of the immediate whitening effect test results is calculated using the following formula:

[0149] Improvement rate (%) = (ITA° value after 2 hours of use - ITA° value before use) / ITA° value before use × 100

[0150] Table 7. Whitening Test ITA° Values

[0151]

[0152] The higher the ITA° value, the brighter the skin; conversely, the lower the ITA° value, the duller the skin. It can reflect the skin's brightening and whitening effect. As can be seen from Table 7, the improvement rate of skin ITA° value before and after use of the essence water made from the plant extracts obtained in all the examples is greater than that of the comparative example, commercially available A, and commercially available B. Therefore, the test results show that the skin ITA° value of the essence water prepared from the plant extracts of the present invention can be significantly improved before and after use. After 2 hours of application, the skin's translucency ITA° value can be improved by more than 8%, which has an immediate whitening and brightening effect.

[0153] Long-lasting whitening effect test:

[0154] The experiment employed a double-blind method with a testing period of 6 weeks. Participants were required to use a facial serum daily, and data was collected from their facial skin four times: at weeks 0, 2, 4, and 6. Thirty-three participants aged 18-30 were recruited and divided into 11 groups of three. Upon the volunteers' first visit, they signed informed consent forms, data was collected at week 0, and samples were distributed. During each visit, volunteers cleansed their faces with a designated cleanser and sat quietly in the testing environment for approximately 20 minutes. After this period, skin melanin and hemoglobin testing probes (Mexameter MX18) were used to collect skin melanin content (MI value) data, as shown in Table 8.

[0155] The melanin change rate from the long-lasting whitening effect test results was calculated using the following formula:

[0156] Melanin change rate (%) = (Melanin content at each time point after product use - Melanin content before product use) / Melanin content before product use × 100.

[0157] Table 8 Results of Melanin Change Rate

[0158]

[0159] A lower melanin change rate indicates lighter skin color and lower melanin content. Therefore, the melanin change rate can be used as an important indicator to evaluate the whitening and spot-fading efficacy of cosmetics. Analysis of the data in Table 8 shows that the skin melanin change rate before and after use of the essence water prepared from the plant extracts obtained in all examples is greater than that of the comparative example, commercially available A, and commercially available B. Therefore, the test results indicate that the essence water prepared from the plant extracts of this invention has a significant skin melanin change rate before and after use, demonstrating its ability to effectively inhibit melanin production, fade spots, and significantly improve skin melanin content after 6 weeks of use, exhibiting a long-lasting whitening effect.

[0160] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A method for preparing a plant extract with immediate and long-lasting whitening, soothing, and anti-inflammatory effects, characterized in that... The following steps are included: (1) Dry the licorice, tea, Scutellaria baicalensis root and mulberry root bark separately, pulverize and sieve them, weigh the pulverized and sieved licorice, tea, Scutellaria baicalensis root and mulberry root bark powders in a mass ratio of 1-5:1-5:1-3:1-3, mix them evenly to obtain mixed powder; (2) Weigh out choline chloride, citric acid and ethylene glycol in a molar ratio of 3:2:1 and mix them. Use a vacuum rotary evaporator to heat the mixture at 70-80℃ for 2-4 h to dissolve it into a uniform liquid solvent. Cool and filter the resulting liquid solvent to obtain a pure DES system. (3) Extraction was performed using a semi-bionic method with the aid of a eutectic solvent. Specifically, the following steps were followed: The mixed powder obtained in step (1) was extracted by reflux with a solution of disodium hydrogen phosphate-citric acid buffer at pH 2.2, 7.4 and 8.0 at a ratio of 1g:15-25mL. Each reflux extraction was performed for 25-35 min, followed by filtration. The filtrate obtained from the three filtrations was retained. The final filter residue was then mixed with the DES system obtained in step (2) at a ratio of 1g:20-35mL. The mixture was extracted at 45-55℃ for 1-3 h and then filtered. The resulting filtrate was mixed with the filtrate obtained from the three filtrations to obtain the DES extract. (4) The DES extract obtained in step (3) is added to the ethylene oxide-propylene oxide copolymer at a mass ratio of 3:2 and shaken to mix. Then, it is centrifuged at 4500-5500 r / min to obtain the upper EOPO enriched phase and the lower DES enriched phase. At this time, the active ingredient is back-extracted from the DES extract into the EOPO enriched phase. After separation, the EOPO enriched phase is placed in a water bath at 60-70℃ for 45-75 min for temperature-induced phase separation. It is centrifuged at 4500-5500 r / min to obtain the EOPO phase and the aqueous phase. At this time, the active ingredient is enriched in the aqueous phase. Then, the aqueous phase is centrifuged at 4500-5500 r / min and filtered with a 0.45-5µm filter membrane. The filtrate is concentrated to a paste by vacuum distillation to obtain a mixture. (5) Spray dry or bake dry the mixture obtained in step (4), mix and pulverize, and sieve to obtain a plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects.

2. The method for preparing a plant extract with immediate and long-lasting whitening, soothing, and anti-inflammatory effects according to claim 1, characterized in that: The drying in step (1) is to dry overnight in a forced-air drying oven at 45-55℃, and the pulverization and sieving is to pass through an 80-mesh sieve; The sieving in step (5) is sieving through an 80-mesh sieve.

3. A plant extract prepared by the method described in claim 1, which has immediate and long-lasting whitening and soothing anti-inflammatory effects.

4. The plant extract with immediate and long-lasting whitening, soothing, and anti-inflammatory effects according to claim 3, characterized in that: The plant extract contains akebia phenylethanol glycoside B, isoliquiritigenin, L-theanine, and 5,7-dihydroxycoumarin.

5. The application of the plant extract with immediate and long-lasting whitening and soothing anti-inflammatory effects as described in claim 3 in the preparation of whitening and soothing anti-inflammatory skin care products.

6. A soothing and anti-inflammatory whitening composition, characterized in that: The composition consists of the plant extract, short-chain alcohol and deionized water as described in claim 3.

7. The method for preparing a soothing and anti-inflammatory whitening composition according to claim 6, characterized in that... The process includes the following steps: the plant extract described in claim 3, short-chain alcohol, and deionized water are prepared in a mass ratio of (1~5):(15~40):(5~45), and then stirred at 300-350 r / min for 10-15 min to obtain a soothing and anti-inflammatory whitening composition; the short-chain alcohol is 1,3-butanediol and / or 1,2-hexanediol.

8. The application of the soothing and anti-inflammatory whitening composition according to claim 6 in the preparation of essence water cosmetics, characterized in that: The essence water cosmetic is prepared according to the following steps: (1) Weigh water, glycerol, disodium EDTA and p-hydroxyacetophenone, mix them evenly, heat to 85-90℃, homogenize for 1-4 min, keep warm for 20 min, and obtain phase A; (2) After the A phase is kept warm, wait for the temperature to drop to 45-50℃, add the whitening composition of claim 6, stir evenly, filter with 400 mesh filter cloth to obtain essence water cosmetic.

Images