Broiler feed for improving muscular stomach development and preparation method thereof
By controlling the particle size of corn particles and adding processed corn, mixed zeolite powder and compound enzyme preparations, combined with allicin and chlorogenic acid, the problems of poor gizzard development, picky eating and mildew in broiler feed are solved, and the effect of improving gizzard development, increasing feed intake and feed conversion rate is achieved.
Patent Information
- Application Number
- CN202511204309.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-27
- Publication Date
- 2025-10-03
- Estimated Expiration
- 2045-08-27
AI Technical Summary
During the preparation process of existing broiler feed, it is difficult to simultaneously improve gizzard development, avoid picky eating behavior, prevent mildew and improve feed conversion rate, resulting in poor growth performance of broilers.
By controlling the particle size of corn particles and adding processed corn, mixed zeolite powder and compound enzyme preparations to the feed, allicin and chlorogenic acid are combined to fix allicin and enhance the gizzard development effect. At the same time, tannic acid and magnesium sulfate heptahydrate are used to improve the thermal stability of the enzyme and prevent mildew.
It improves the development of gizzard, increases the feed intake of broilers, avoids picky eating behavior, prevents feed mold, improves feed conversion rate and feces quality, and promotes the healthy growth of broilers.
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of broiler feed, and in particular to a broiler feed capable of improving gizzard development and a preparation method thereof. Background Art
[0002] In broiler production, feed particle size should be appropriate. When the particle size is too coarse, the larger particles pass through the gizzard relatively slowly, making it more difficult for the broiler's digestive tract to digest the feed. This conflicts with the broiler's nutritional needs for rapid growth, resulting in slower weight gain. When the particle size is too fine, dust is easily produced, causing feed waste. Moreover, when fine feed passes through the broiler's gizzard, due to its rapid digestion rate, the gizzard cannot perform its physical digestive function, which can lead to gizzard atrophy over time. At the same time, a large amount of feed retained in the broiler's small intestine in a short period of time can easily lead to small intestinal hypertrophy, affecting the digestion and absorption function of the small intestine, and further leading to a decrease in feed conversion rate.
[0003] Zhong Limei. Effects of wheat particle size under different feed forms on broiler growth performance and intestinal health. Master's thesis of Sichuan Agricultural University. Published in June 2016, it was shown that the particle size of feed has a certain impact on nutrient utilization, digestive organ development, immune function, and intestinal mechanical barrier of broilers. In addition, the form of feed also has an impact on broilers. Powdered feed easily leads to feed grading, resulting in nutritional imbalance in animal feeding. Pellet feed is based on powdered feed and is obtained through granulation. Compared with powdered feed, pelleted feed has the advantages of convenient transportation, increased feed intake, and reduced raw material grading. Moreover, the high temperature and high pressure environment during granulation can kill pathogenic microorganisms in the powdered feed. Shi Zhanyue, Deng Hongcheng, Wang Ming, Guo Qiusong, Zheng Jinhuan, Situ Jinshui, Xie Gaomiao, Yang Xiaoyang, and Shen Weijun. Effects of corn particle size on broiler performance and digestive tract parameters. China Animal Husbandry Journal, November 2017. Corn is ground into corn pellets, mixed with other feed ingredients, and pelleted to produce pelleted feed. The particle size of corn during grinding affects the gizzard weight index, ileum weight index, small intestine weight index, ileum length index, and small intestine length index of broilers. Gou Zhongyong. Effects of feed shape and coarsely ground corn kernels on broiler performance, weight consistency, relative gizzard weight, fecal nitrogen, and feed particle size preference. Guangdong Feed, May 2016. Feed intake in the crumbled diet group was significantly higher than that in the mash diet group, and broilers in the crumbled diet group had significantly higher body weight than those in the mash diet group. However, the above research mainly targets broilers in the growing period. Zhao Danyang, Li Junguo, Qin Yuchang, Gu Xu, Yang Jie, Dong Yingchao, Xue Yong, Li Fuhuang, Li Zheng, and Li Jun. Effects of different crushing particle size combinations of corn and soybean meal on pellet processing quality and broiler growth performance. Journal of Animal Nutrition. October 2019 published that the digestive organs of chicks are not yet fully developed and cannot crush larger particles, so the crushing particle size will affect the growth performance of young birds. Therefore, the experimental subjects are mainly broilers.
[0004] In summary, when preparing feed for growing broiler chickens, wheat or corn is often ground into wheat or corn granules, mixed with other feed ingredients, and pelletized to produce pelleted feed. However, Shi Zhanyue, Deng Hongcheng, Wang Ming, Guo Qiusong, Zheng Jinhuan, Situ Jinshui, Xie Gaomiao, Yang Xiaoyang, and Shen Weijun (Effects of Corn Particle Size on Broiler Production Performance and Digestive Tract Indicators, China Animal Husbandry Journal, November 2017) also reported that excessively large corn granules can lead to poor pellet quality, increase the pellet pulverization rate, and make broilers more picky eaters, further impacting their feed intake. However, the gizzard weight index decreases with decreasing corn granule size. Therefore, it is difficult to simultaneously increase broiler feed intake and gizzard weight index by controlling corn granule size.
[0005] A well-developed gizzard is associated with enhanced gastric glandular function, which can lead to a number of positive responses in broilers, including lowering gizzard pH, increasing pepsin activity, increasing satiety, and prolonging the retention of feed particles in the foregut, thereby increasing exposure to the digestive process and controlling the rate at which chyme flows into the lumen. Studies have shown that a well-developed gizzard results in more uniform and controlled movement of chyme particles entering the small intestine, thereby reducing the possibility of starch overload in the lumen and, further, improving feed conversion efficiency. Therefore, a reduced gizzard weight index can lead to a reduced feed conversion efficiency.
[0006] Fu Yanan and Mark Clements. Adding whole grains to broiler diets improves feed utilization. Foreign Animal Husbandry (Swine and Poultry). In October 2018, the paper published that pelletized feed, when added as a separate ingredient to pelleted feed, can stimulate broiler chickens' appetites. However, once ingested, pelleted feed is easily decomposed and minimally irritates the gizzard. Long-term use can reduce the digestive capacity of broilers. Whole grains, on the other hand, can induce a larger gizzard reaction, increasing gizzard capacity and efficiency. Therefore, the applicants attempted to add large-particle corn (hereinafter referred to as large-particle corn) to pelleted feed to produce a broiler feed that improves gizzard development. This feed was then used in rearing broilers. However, the following problems were discovered: First, Fu Yanan and Mark Clements. Adding whole grains to broiler diets can improve feed utilization. Foreign Animal Husbandry (Swine and Poultry). In October 2018, it was published that feeding with whole grains can cause a larger response in the gizzard, including gizzard volume and efficiency. However, broilers will refuse to eat whole grains and scrape them out of the feed trough. Therefore, this feeding method requires an adaptation period. Gan Yuening, Song Zhifang, Wang Junliang, Li Jinge, Su Manchun, Sun Duanyang, Yang Tao, and Gong Yuesheng. Effects of corn powder particle size on production performance and egg quality of Shaanxi Lueyang black-bone chickens. Feed Research. October 2014. Chickens are picky eaters, preferring to first peck at large particles in the powdered feed, then smaller particles, and finally the fine powder at the bottom of the feed trough. They prefer coarse particles first. When a certain amount of feed accumulates in the stomach, the chicken's sense of fullness increases, and their feeding decreases or even stops. Fine powder, however, due to its smaller particles, has a weaker sense of fullness, leading to a corresponding increase in feed intake. Furthermore, under the same digestive conditions, the gizzard requires longer to contract and grind coarse powder than fine powder. This increases feed circulation time in the gastrointestinal tract, slowing its emptying rate. Consequently, the chicken's hunger is less intense, resulting in a weaker appetite and lower feed intake. In summary, since the pellet feed reduces the anti-nutritional factors in the feed through high temperature during preparation and gelatinizes the starch in the feed to produce a unique aroma, it can increase the appetite of broilers and increase the feed intake of broilers. Therefore, after the applicant uses the prepared broiler feed for improving gizzard development for broilers, the broilers will show picky eating behavior and preferentially peck at the pellet feed, resulting in an insignificant effect on improving gizzard development.
[0007] Second, pellet feed preparation is prone to problems such as incomplete drying and high moisture content. Chen Yunfeng, "Analysis and Solutions for Three Typical Moldy Phenomena in Pellets," Guangdong Feed, January 2014, published three typical moldy phenomena in pellet feed, their causes, and solutions. Large corn granules contain anti-nutritional factors and are also susceptible to moisture. After using the broiler feed designed to enhance gizzard development on growing broilers, the applicant discovered that the broiler feces were of poor quality, characterized by loose stools and the presence of free water. Furthermore, the applicant discovered that the broiler feed was susceptible to mold during storage.
[0008] In response to the above problems, research has found that the commonly used solutions are: first, control the particle size of pellet feed and large-particle corn, and keep the particle size of pellet feed consistent with that of large-particle corn; second, thoroughly dry the pellet feed and large-particle corn; third, Xi Haibo. Application of compound enzyme preparations in broilers. Master's thesis of Northwest Agriculture and Forestry University. In June 2005, it was disclosed that adding compound enzyme preparations to feed can eliminate multiple anti-nutritional factors. Therefore, adding compound enzyme preparations to pellet feed can reduce the impact of anti-nutritional factors in large-particle corn.
[0009] However, after the applicant applied the above three solutions simultaneously to the preparation of broiler feed for improving gizzard development, it was found that the following problems still existed: First, due to the different odors of pelleted feed and large-particle corn, there is still a picky eating problem after being used for broiler breeding, which further leads to the insignificant effect on improving gizzard development; Second, high-temperature treatment is required during pelleting, and the heat resistance of the complex enzyme preparation is poor, which will affect the activity of the complex enzyme preparation, further affecting the effectiveness of the use of the complex enzyme activity. Summary of the Invention
[0010] In response to the deficiencies in the prior art, the present invention provides a broiler feed for improving gizzard development and a preparation method thereof. The prepared broiler feed can improve the gizzard development of broilers while also avoiding the occurrence of mildew, thereby improving the feed conversion rate and feces quality of broilers.
[0011] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows: A method for preparing broiler feed for improving gizzard development, comprising: crushing corn, processing corn, premixing, mixing, crushing, granulating, and mixing; The crushed corn is prepared by crushing dry corn kernels and passing them through a 7-8 mm sieve, taking the undersize, passing the undersize through a 2.5-3 mm sieve, taking the oversize, and drying at 60-70° C. to constant weight to obtain the crushed corn; In the crushed corn, the moisture content of the dry corn kernels is 12-15%; The treated corn comprises mixing crushed corn, chlorogenic acid, and the first portion of water, ultrasonically treating the mixture for 30-40 minutes, filtering, taking the filter residue, washing it 2-3 times with water, and drying the filter residue at 60-70° C. to constant weight to obtain pretreated corn; mixing garlic cloves and the second portion of water, crushing and beating the mixture, passing it through a 30-mesh sieve, taking the sieve residue, mixing the mixture with the pretreated corn, stirring the mixture for 30-40 minutes at a stirring speed of 50-150 rpm, standing the mixture for 2-3 hours, passing the mixture through a 2.5-3 mm sieve, taking the sieve residue, washing it 2-3 times with water, and vacuum freeze-drying the mixture to obtain the treated corn; In the treated corn, the weight ratio of crushed corn, chlorogenic acid, and water is 1000-1100:5-6:6000-7000; The weight ratio of crushed corn, garlic cloves, and the second portion of water is 1000-1100:25-27:2000-2500; The mass content of chlorogenic acid in the chlorogenic acid is 70%; The water content of the garlic clove is 64%; The ultrasonic power in the ultrasonic treatment is 500W and the ultrasonic frequency is 25kHz; The temperature during the vacuum freeze drying is -50°C to -45°C, and the time is 24-26 hours; The premixing comprises mixing zeolite powder, tannic acid and the first portion of water, stirring for 50-60 minutes at a stirring speed of 50-150 rpm, keeping the stirring speed constant, adjusting the pH to 8.5-9, stirring for 10-12 hours, centrifuging for 5-10 minutes at a centrifugal speed of 3000-4000 rpm, taking a precipitate, mixing with the second portion of water, adding phytase and complex enzyme, adjusting the pH to 6-6.5, stirring at 34-37° C. for 4-5 hours at a stirring speed of 20-50 rpm, centrifuging for 5-10 minutes at a centrifugal speed of 3000-4000 rpm, taking a precipitate, and vacuum freeze-drying to obtain the premixed zeolite powder; In the premix, the weight ratio of zeolite powder, tannic acid, and the first portion of water is 200-210:5-6:2000-2200; The weight ratio of zeolite powder, the second portion of water, phytase, and complex enzyme is 200-210:2000-2200:7-7.3:5.4-5.6; The average particle size of the zeolite powder is 0.8 mm; The temperature during the vacuum freeze drying is -50°C to -45°C, and the time is 30-35h; The activity of the phytase is 9000-11000 U / g; The complex enzyme consists of xylanase, β-glucanase, cellulase, amylase, protease, mannanase and glucose oxidase, wherein the activity of xylanase is 7000-10000U / g, the activity of β-glucanase is 9000-11000U / g, the activity of cellulase is 20-50U / g, the activity of amylase is 1000-3000U / g, the activity of protease is 900-1100U / g, the activity of mannanase is 1500-2500U / g, and the activity of glucose oxidase is 9000-11000U / g; The mixing step comprises mixing corn starch, magnesium sulfate heptahydrate, and water, stirring the mixture at 25-30° C. for 50-60 minutes at a stirring speed of 50-150 rpm, maintaining the stirring speed constant, adjusting the pH to 4.5-5, adding pretreated zeolite powder, stirring the mixture for 1-2 hours, allowing the mixture to stand at 25-30° C. for 8-9 hours, centrifuging the mixture for 5-10 minutes at a centrifugal speed of 3000-4000 rpm, collecting the precipitate, and freeze-drying the mixture in vacuum to obtain a mixed zeolite powder; In the mixture, the weight ratio of corn starch, magnesium sulfate heptahydrate, and water is 6.2-7:15-16:900-1000; The weight ratio of the corn starch in the mixture to the zeolite powder in the premix is 6.2-7:200-210; The temperature during the vacuum freeze drying is -50°C to -45°C, and the time is 30-35h; The pulverizing comprises pulverizing corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine, and then passing the pulverized corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine through a 50-80 mesh sieve to obtain pulverized corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine; The mass content of protein in the corn gluten meal is 60%; The mass content of lysine in the L-lysine sulfate is 65%; The mass content of sodium humate in the sodium humate is 65%; The mass content of choline chloride in the choline chloride is 70%; The granulation comprises the following steps: mixing, by weight, ground corn flour, ground soybean meal, ground flour, ground peanut meal, ground corn gluten meal, ground enzymatically hydrolyzed feather meal, ground monosodium glutamate residue, ground bone meal, ground L-lysine sulfate, ground bone calcium hydrogen phosphate, ground sodium humate, ground methionine hydroxy analogue, ground DL-methionine, ground sodium chloride, ground L-threonine, ground sodium bicarbonate, ground choline chloride, ground L-arginine, ground L-valine, duck fat, mixed zeolite powder, a first premix, and a second premix, followed by granulation, and drying until the moisture content is less than 12.5% to obtain pellet feed; In the granulation, the crushed corn flour, the crushed soybean meal, the crushed flour, the crushed peanut meal, the crushed corn gluten meal, the crushed enzymatic hydrolyzed feather meal, the crushed monosodium glutamate residue, the crushed bone meal, the crushed L-lysine sulfate, the crushed bone calcium hydrogen phosphate, the crushed sodium humate, the crushed methionine hydroxy analogue, the crushed DL-methionine, the crushed sodium chloride, the crushed L-threonine, the crushed sodium bicarbonate, the crushed choline chloride, the crushed L-arginine, the crushed L-valine, the duck fat, the mixed zeolite powder, the first premix, The weight ratio of the second premix is 24-25:24-24.5:8-8.3:4-4.2:3-3.2:2.5-2.6:1-1.1:1-1.2:0.7-0.75:0.3-0.33:0.2-0.21:0.2-0.21:0.16-0.17:0.15-0.16:0.14-0.15:0.13-0.14:0.08-0.09:0.07-0.08:0.04-0.05:8-8.4:1.2-1.3:0.1-0.11:0.2-0.22; The first premix is a broiler chicken compound premix feed BV22 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., wherein the carrier is rice husk powder, the water content is less than 10%, the content of vitamin A acetate is 7000-10000 KIU / kg, the content of vitamin D3 is 2000-4400 KIU / kg, the content of DL-α-tocopheryl acetate is ≥25000 mg / kg, the content of ammonium sulfate nitrate is ≥2160 mg / kg, the content of menadione is ≥2160 mg / kg, the content of nicotinamide is ≥28800 mg / kg, the content of D-pantothenic acid is ≥8640 mg / kg, and the content of riboflavin is ≥6480 mg / kg. The second premix is a poultry trace element premix feed M-B2 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with a carrier of stone powder, a moisture content of less than 10%, a copper content of 1920-20000 mg / kg, an iron content of 19600-200000 mg / kg, a manganese content of 23200-100000 mg / kg, and a zinc content of 23600-120000 mg / kg; The diameter of the pellet feed is 3-4 mm and the length is 10-12 mm; The mixture is prepared by uniformly mixing 24-27 parts of processed corn and 78-82 parts of pelleted feed by weight to obtain a broiler feed for improving gizzard development.
[0012] A broiler feed for improving gizzard development prepared by the above-mentioned preparation method.
[0013] Compared with the prior art, the present invention has the following beneficial effects: (1) The method for preparing broiler feed for improving gizzard development of the present invention comprises adding treated corn and mixed zeolite powder during the preparation process. When preparing the treated corn, the corn is first crushed and dried to obtain crushed corn. The crushed corn is then mixed with chlorogenic acid and ultrasonically treated. Chlorogenic acid combines with starch on the surface of the crushed corn under the action of ultrasound. The chlorogenic acid is then mixed with garlic paste. Allicin in the garlic paste can combine with chlorogenic acid and corn starch, thereby fixing and encapsulating allicin. Chlorogenic acid can improve the fixation and encapsulation of allicin and also play an anti-mildew role, thereby obtaining treated corn with allicin bound to the surface. Allicin can play an appetizer role. The invention relates to a method for preparing a broiler feed containing tannic acid and phytase, wherein the tannic acid is adsorbed on the surface of the zeolite powder, thereby improving the binding effect of the phytase and the complex enzyme. The zeolite powder is then combined with corn starch and magnesium sulfate heptahydrate. The corn starch and tannic acid can be bound to each other through intermolecular forces. The tannic acid can also complex the magnesium sulfate heptahydrate. The corn starch as a polysaccharide and the magnesium sulfate heptahydrate as an inorganic salt can both improve the thermal stability of the phytase and the complex enzyme. Furthermore, the activity of the phytase and the complex enzyme in the prepared broiler feed for improving gizzard development is improved. (2) The broiler feed prepared by the present invention can improve the gizzard development of broiler chickens. For 21-day-old white-feathered broilers, the average weight of the gizzards can reach 28.5-28.7 g after 15 consecutive days of feeding; (3) The broiler feed prepared by the present invention can avoid the occurrence of mildew; (4) The broiler feed prepared by the present invention can improve the feed conversion rate of broilers, and for 21-35 day old white-feathered broilers, the feed-to-meat ratio can be reduced to 1.32; (4) The broiler feed prepared by the present invention can improve the feces quality of broiler chickens. DETAILED DESCRIPTION
[0014] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific embodiments of the present invention are now described.
[0015] Example 1 This embodiment provides a method for preparing a broiler feed for improving gizzard development, which is as follows: 1. Grinding corn: Grind dry corn kernels and pass them through a 7 mm sieve. Take the undersize, pass the undersize through a 2.5 mm sieve, take the oversize, and dry at 60°C to constant weight to obtain the ground corn. The moisture content of the dry corn kernels is 14%; 2. Treat corn: Take 1000g crushed corn, 5g chlorogenic acid, and 6000g water, mix them, and ultrasonically treat them for 30 minutes. The ultrasonic power during the ultrasonic treatment is 500W and the ultrasonic frequency is 25kHz. Filter, take the filter residue, wash it twice with water, and the amount of water used in each washing is 3000g. Dry the filter residue at 60°C to constant weight to obtain pretreated corn; mix 25g garlic cloves and 2000g water, crush and pulp them, pass them through a 30-mesh sieve, take the sieve underfill, mix them with all the pretreated corn mentioned above, stir them for 30 minutes, and the stirring speed during the stirring treatment is 100rpm. Let it stand for 2h, pass it through a 2.5mm sieve, take the sieve overfill, wash it twice with water, and the amount of water used in each washing is 3000g. Vacuum freeze-dry it at -50°C for 24h to obtain treated corn; The mass content of chlorogenic acid in the chlorogenic acid is 70%; The water content of the garlic clove is 64%; 3. Premixing: 200 g of zeolite powder, 5 g of tannic acid, and 2000 g of water were mixed, stirred for 50 min at a stirring speed of 50 rpm, the stirring speed was kept constant, the pH was adjusted to 8.5, stirred for 10 h, centrifuged for 5 min at a centrifugal speed of 3000 rpm, the precipitate was taken, mixed with 2000 g of water, 7 g of phytase and 5.4 g of complex enzyme were added, the pH was adjusted to 6, stirred at 34° C. for 4 h at a stirring speed of 20 rpm, centrifuged for 5 min at a centrifugal speed of 3000 rpm, the precipitate was taken, and vacuum freeze-dried at -50° C. for 30 h to obtain premixed zeolite powder; The average particle size of the zeolite powder is 0.8 mm; The activity of the phytase is 10000 U / g; The complex enzyme consists of xylanase, β-glucanase, cellulase, amylase, protease, mannanase and glucose oxidase, wherein the activity of xylanase is 8000U / g, the activity of β-glucanase is 10000U / g, the activity of cellulase is 30U / g, the activity of amylase is 2000U / g, the activity of protease is 1000U / g, the activity of mannanase is 2000U / g, and the activity of glucose oxidase is 10000U / g; 4. Mixing: 6.2 g corn starch, 15 g magnesium sulfate heptahydrate, and 900 g water were mixed and stirred at 25° C. for 50 min at a stirring speed of 100 rpm. The stirring speed was kept constant, and the pH was adjusted to 4.5. All the pretreated zeolite powder obtained in step 3 was added and stirred for 1 h. The mixture was allowed to stand at 25° C. for 8 h and centrifuged for 5 min at a centrifugal speed of 3000 rpm. The precipitate was collected and freeze-dried in a vacuum at -50° C. for 30 h to obtain a mixed zeolite powder. 5. Grinding: Grind corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine, and pass through a 50-mesh sieve to obtain ground corn flour, ground soybean meal, ground flour, ground peanut meal, ground corn gluten meal, ground enzymatically hydrolyzed feather meal, ground monosodium glutamate residue, ground bone meal, ground L-lysine sulfate, ground bone dibasic calcium phosphate, ground sodium humate, ground methionine hydroxy analogue, ground DL-methionine, ground sodium chloride, ground L-threonine, ground sodium bicarbonate, ground choline chloride, ground L-arginine, and ground L-valine; The mass content of protein in the corn gluten meal is 60%; The mass content of lysine in the L-lysine sulfate is 65%; The mass content of sodium humate in the sodium humate is 65%; The mass content of choline chloride in the choline chloride is 70%; 6. Granulation: According to weight, 24 parts of ground corn flour, 24 parts of ground soybean meal, 8 parts of ground flour, 4 parts of ground peanut meal, 3 parts of ground corn gluten meal, 2.5 parts of ground enzymatic feather meal, 1 part of ground monosodium glutamate residue, 1 part of ground bone meal, 0.7 parts of ground L-lysine sulfate, 0.3 parts of ground bone calcium hydrogen phosphate, 0.2 parts of ground sodium humate, 0.2 parts of ground methionine hydroxy analogue, 0.16 parts of ground DL-methionine, 0.15 parts of crushed sodium chloride, 0.14 parts of crushed L-threonine, 0.13 parts of crushed sodium bicarbonate, 0.08 parts of crushed choline chloride, 0.07 parts of crushed L-arginine, 0.04 parts of crushed L-valine, 8 parts of duck fat, 1.2 parts of mixed zeolite powder, 0.1 parts of the first premix, and 0.2 parts of the second premix are mixed and granulated, and dried to a moisture content of 10.2% to obtain a pellet feed with a diameter of 3 mm and a length of 10 mm; The first premix is a broiler chicken compound premix feed BV22 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with rice husk powder as the carrier, a water content of 9.1%, a vitamin A acetate content of 10,000 KIU / kg, a vitamin D3 content of 4,400 KIU / kg, a DL-α-tocopheryl acetate content of 26,000 mg / kg, an ammonium sulfate content of 2,300 mg / kg, a menadione content of 2,300 mg / kg, a nicotinamide content of 30,000 mg / kg, a D-pantothenic acid content of 8,800 mg / kg, and a riboflavin content of 6,500 mg / kg. The second premix is a poultry trace element premix feed M-B2 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with a carrier of stone powder, a water content of 8.8%, a copper content of 10,000 mg / kg, an iron content of 120,000 mg / kg, a manganese content of 80,000 mg / kg, and a zinc content of 90,000 mg / kg; 7. Mixing: According to weight, 24 parts of treated corn and 78 parts of pelleted feed are mixed evenly to obtain a broiler feed for improving gizzard development.
[0016] This embodiment also provides a broiler feed for improving gizzard development, which is prepared by the aforementioned preparation method.
[0017] Example 2 This embodiment provides a method for preparing a broiler feed for improving gizzard development, which is as follows: 1. Grinding corn: Grind dry corn kernels and pass them through an 8 mm sieve. Take the undersize, pass the undersize through a 3 mm sieve, take the oversize, and dry at 70°C to constant weight to obtain the ground corn. The moisture content of the dry corn kernels is 14%; 2. Processing corn: 1100g of crushed corn, 6g of chlorogenic acid, and 7000g of water were mixed and ultrasonically treated for 40min. The ultrasonic power during the ultrasonic treatment was 500W and the ultrasonic frequency was 25kHz. The residue was filtered and washed with water three times. The amount of water used in each washing was 4000g. The residue was dried at 70°C to constant weight to obtain pretreated corn; 27g of garlic cloves and 2500g of water were mixed, crushed and pulped, passed through a 30-mesh sieve, and the undersize was taken. After mixing with all the pretreated corn mentioned above, the mixture was stirred for 40min at a stirring speed of 150rpm. The mixture was allowed to stand for 3h, passed through a 3mm sieve, and the oversize was washed with water three times. The amount of water used in each washing was 4000g. The mixture was vacuum freeze-dried at -45°C for 26h to obtain treated corn; The mass content of chlorogenic acid in the chlorogenic acid is 70%; The water content of the garlic clove is 64%; 3. Premixing: 210 g of zeolite powder, 6 g of tannic acid, and 2200 g of water were mixed, stirred for 60 min at a stirring speed of 150 rpm, the stirring speed was kept constant, the pH was adjusted to 9, stirred for 12 h, centrifuged for 10 min at a centrifugal speed of 4000 rpm, the precipitate was taken, mixed with 2200 g of water, 7.3 g of phytase and 5.6 g of complex enzyme were added, the pH was adjusted to 6.5, stirred at 37 ° C for 5 h at a stirring speed of 50 rpm, centrifuged for 10 min at a centrifugal speed of 4000 rpm, the precipitate was taken, and vacuum freeze-dried at -45 ° C for 35 h to obtain premixed zeolite powder; The average particle size of the zeolite powder is 0.8 mm; The activity of the phytase is 10000 U / g; The complex enzyme consists of xylanase, β-glucanase, cellulase, amylase, protease, mannanase and glucose oxidase, wherein the activity of xylanase is 8000U / g, the activity of β-glucanase is 10000U / g, the activity of cellulase is 30U / g, the activity of amylase is 2000U / g, the activity of protease is 1000U / g, the activity of mannanase is 2000U / g, and the activity of glucose oxidase is 10000U / g; 4. Mixing: 7 g corn starch, 16 g magnesium sulfate heptahydrate, and 1000 g water were mixed and stirred at 30° C. for 60 min at a stirring speed of 150 rpm. The stirring speed was kept constant, and the pH was adjusted to 5. All the pretreated zeolite powder obtained in step 3 was added and stirred for 2 h. The mixture was allowed to stand at 30° C. for 9 h and centrifuged for 10 min at a centrifugal speed of 4000 rpm. The precipitate was collected and freeze-dried in a vacuum at -45° C. for 35 h to obtain a mixed zeolite powder. 5. Grinding: Grind corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine, and pass through an 80-mesh sieve to obtain ground corn flour, ground soybean meal, ground flour, ground peanut meal, ground corn gluten meal, ground enzymatically hydrolyzed feather meal, ground monosodium glutamate residue, ground bone meal, ground L-lysine sulfate, ground bone dibasic calcium phosphate, ground sodium humate, ground methionine hydroxy analogue, ground DL-methionine, ground sodium chloride, ground L-threonine, ground sodium bicarbonate, ground choline chloride, ground L-arginine, and ground L-valine; The mass content of protein in the corn gluten meal is 60%; The mass content of lysine in the L-lysine sulfate is 65%; The mass content of sodium humate in the sodium humate is 65%; The mass content of choline chloride in the choline chloride is 70%; 6. Granulation: by weight, 25 parts of ground corn flour, 24.5 parts of ground soybean meal, 8.3 parts of ground flour, 4.2 parts of ground peanut meal, 3.2 parts of ground corn gluten meal, 2.6 parts of ground enzymatic feather meal, 1.1 parts of ground monosodium glutamate residue, 1.2 parts of ground bone meal, 0.75 parts of ground L-lysine sulfate, 0.33 parts of ground bone calcium hydrogen phosphate, 0.21 parts of ground sodium humate, 0.21 parts of ground methionine hydroxy analogue, 0. 0.17 parts of crushed DL-methionine, 0.16 parts of crushed sodium chloride, 0.15 parts of crushed L-threonine, 0.14 parts of crushed sodium bicarbonate, 0.09 parts of crushed choline chloride, 0.08 parts of crushed L-arginine, 0.05 parts of crushed L-valine, 8.4 parts of duck fat, 1.3 parts of mixed zeolite powder, 0.11 parts of the first premix, and 0.22 parts of the second premix are mixed and granulated, and dried to a moisture content of 11% to obtain a pellet feed with a diameter of 4 mm and a length of 12 mm; The first premix is a broiler chicken compound premix feed BV22 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with rice husk powder as the carrier, a water content of 9.1%, a vitamin A acetate content of 10,000 KIU / kg, a vitamin D3 content of 4,400 KIU / kg, a DL-α-tocopheryl acetate content of 26,000 mg / kg, an ammonium sulfate content of 2,300 mg / kg, a menadione content of 2,300 mg / kg, a nicotinamide content of 30,000 mg / kg, a D-pantothenic acid content of 8,800 mg / kg, and a riboflavin content of 6,500 mg / kg. The second premix is a poultry trace element premix feed M-B2 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with a carrier of stone powder, a water content of 8.8%, a copper content of 10,000 mg / kg, an iron content of 120,000 mg / kg, a manganese content of 80,000 mg / kg, and a zinc content of 90,000 mg / kg; 7. Mixing: According to weight, 27 parts of treated corn and 82 parts of pellet feed are mixed evenly to obtain a broiler feed for improving gizzard development.
[0018] This embodiment also provides a broiler feed for improving gizzard development, which is prepared by the aforementioned preparation method.
[0019] Comparative Example 1 Based on the method for preparing a broiler feed for improving gizzard development in Example 1, the second step of processing corn is omitted, and an equal weight portion of crushed corn obtained by crushing the corn in the first step is used in the original mixing in the seventh step to replace the processed corn.
[0020] The remaining operations remain the same as in Example 1.
[0021] Comparative Example 2 Based on the preparation method of a broiler feed for improving gizzard development in Example 1, step 4 of mixing is omitted, and in the original step 6 of granulation, an equal weight portion of the premixed zeolite powder obtained by premixing in step 3 is used instead of the mixed zeolite powder.
[0022] The remaining operations remain the same as in Example 1.
[0023] Comparative Example 3 Based on the method for preparing a broiler feed for improving gizzard development in Example 1, the pre-mixing step 3 was omitted, and in the original mixing step 4, a mixture of 200 g of zeolite powder, 7 g of phytase, and 5.4 g of complex enzyme was used instead of the entire pre-treated zeolite powder obtained in step 3; The average particle size of the zeolite powder is 0.8 mm; The activity of the phytase is 10000 U / g; The complex enzyme consists of xylanase, β-glucanase, cellulase, amylase, protease, mannanase and glucose oxidase, wherein the activity of the xylanase is 8000 U / g, the activity of the β-glucanase is 10000 U / g, the activity of the cellulase is 30 U / g, the activity of the amylase is 2000 U / g, the activity of the protease is 1000 U / g, the activity of the mannanase is 2000 U / g, and the activity of the glucose oxidase is 10000 U / g.
[0024] The remaining operations remain the same as in Example 1.
[0025] Test Example 1 The preparation method of the conventional broiler feed used in this test example is as follows: According to weight, 48 parts of ground corn flour, 24 parts of ground soybean meal, 8 parts of ground flour, 4 parts of ground peanut meal, 3 parts of ground corn gluten meal, 2.5 parts of ground enzymatic feather meal, 1 part of ground monosodium glutamate residue, 1 part of ground bone meal, 0.7 parts of ground L-lysine sulfate, 0.3 parts of ground bone calcium hydrogen phosphate, 0.2 parts of ground sodium humate, 0.2 parts of ground methionine hydroxy analogue, 0.1 6 parts of crushed DL-methionine, 0.15 parts of crushed sodium chloride, 0.14 parts of crushed L-threonine, 0.13 parts of crushed sodium bicarbonate, 0.08 parts of crushed choline chloride, 0.07 parts of crushed L-arginine, 0.04 parts of crushed L-valine, 8 parts of duck fat, 1.2 parts of zeolite powder, 0.2 parts of the first premix, and 0.2 parts of the second premix are mixed and granulated to obtain a pellet feed with a diameter of 3 mm and a length of 10 mm; The pulverization method of the above-mentioned pulverized raw materials is the same as that of Example 1; The mass content of protein in the corn gluten meal is 60%; The mass content of lysine in the L-lysine sulfate is 65%; The mass content of sodium humate in the sodium humate is 65%; The mass content of choline chloride in the choline chloride is 70%; The average particle size of the zeolite powder is 0.8 mm; The first premix is a broiler chicken compound premix feed BV22 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with rice husk powder as the carrier, a water content of 9.1%, a vitamin A acetate content of 10,000 KIU / kg, a vitamin D3 content of 4,400 KIU / kg, a DL-α-tocopheryl acetate content of 26,000 mg / kg, an ammonium sulfate content of 2,300 mg / kg, a menadione content of 2,300 mg / kg, a nicotinamide content of 30,000 mg / kg, a D-pantothenic acid content of 8,800 mg / kg, and a riboflavin content of 6,500 mg / kg. The second premix is the poultry trace element premix feed M-B2 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., the carrier is stone powder, the water content is 8.8%, the copper content is 10,000 mg / kg, the iron content is 120,000 mg / kg, the manganese content is 80,000 mg / kg, and the zinc content is 90,000 mg / kg.
[0026] The broiler feeds for improving gizzard development of Examples 1-2 and Comparative Examples 1-3, as well as conventional broiler feeds, were used to raise white-feathered broilers in the growing period. Specifically, 3,000 21-day-old white-feathered broilers were selected and raised in the same chicken house in cages, with 10 white-feathered broilers in each cage. All the white-feathered broilers were evenly divided into 6 groups, labeled Groups 1-6, and the average weight of each white-feathered broiler in Groups 1-6 was calculated. The calculation results are as follows:
[0027] Then, groups 1-5 were fed with the broiler feed for improving gizzard development of Examples 1-2 and Comparative Examples 1-3, respectively. Group 6 was used as a control. Group 6 was fed with conventional broiler feed. Groups 1-6 were all fed ad libitum. The initial temperature of the chicken house was controlled at 25°C. During feeding, the temperature was reduced by 0.3°C every day, and the relative humidity of the chicken house was controlled at 55%. The chickens were fed continuously for 15 days, and the feed intake and weight of each group of white-feathered broilers were recorded every day. After 15 consecutive days of feeding, the average feed intake and average weight of each group of white-feathered broilers were calculated, and the calculation results are as follows:
[0028] The average feed to meat ratio is calculated based on the average feed intake and average weight. The results are as follows:
[0029] After 15 consecutive days of feeding, 20 white-feathered broiler chickens were randomly selected from groups 1-6, slaughtered, and the gizzards were weighed. The average weight of the gizzards in each group was calculated. The results are as follows:
[0030] During the continuous feeding period, the feed of groups 1-5 of white-feathered broilers was observed at 5 pm every day after eating. The amount of corn kernels in the remaining feed was used to determine whether there was a picky eating problem. Finally, statistics were counted to see whether there was a picky eating problem in groups 1-5 of white-feathered broilers. The statistical results are as follows:
[0031] At the same time, the feces of each group of white-feathered broiler chickens were scored every day during the feeding period. The scores and corresponding scoring standards are as follows:
[0032] The feces of each group of white-feathered broiler chickens were collected and scored every day. After 15 consecutive days of feeding, the average feces score of each group of white-feathered broiler chickens during the feeding period was calculated. The calculation results are as follows:
[0033] It can be seen from the results of this test example that the broiler feed for improving gizzard development prepared in Example 1 and Example 2 has the best comprehensive effect in reducing feed-to-weight ratio, increasing weight gain of white-feathered broilers, avoiding picky eating of white-feathered broilers, and improving feces quality of white-feathered broilers.
[0034] Test Example 2 500 g of each of the broiler feeds for improving gizzard development prepared in Examples 1-2 and Comparative Examples 1-3 were taken and sealed in sealed bags. After checking that the sealing was good, the sealed broiler feeds were placed in the sun for 1 hour at 2:00 p.m. every day from June 15, 2025 to July 15, 2025, and then brought indoors. The indoor temperature was maintained at 25° C. and the relative humidity was maintained at 50%. The sealed broiler feeds were then observed for mold at 10:00 a.m. on July 16. The observation results are as follows:
[0035] It can be seen from the results of this test example that the broiler feed for improving gizzard development prepared in Comparative Example 1 has a poor anti-mildew effect.
Claims
1. A method for preparing a broiler feed for improving gizzard development, characterized in that: include: Crush corn, process corn, pre-mix, blend, crush, pelletize, mix; The crushed corn comprises crushing dry corn kernels and passing them through a 7-8 mm sieve, taking the undersize, passing the undersize through a 2.5-3 mm sieve, taking the oversize, and drying to obtain the crushed corn; The treated corn comprises mixing crushed corn, chlorogenic acid, and the first portion of water, performing ultrasonic treatment, filtering, collecting the filter residue, washing, and drying to obtain pretreated corn; mixing garlic cloves and the second portion of water, crushing and beating the mixture, passing the mixture through a 30-mesh sieve, collecting the residue below the sieve, mixing the mixture with the pretreated corn, stirring, allowing the mixture to stand, passing the mixture through a 2.5-3 mm sieve, collecting the residue above the sieve, washing, and vacuum freeze-drying to obtain the treated corn; The premixing comprises mixing zeolite powder, tannic acid, and the first portion of water, stirring, adjusting the pH to 8.5-9, stirring, centrifuging, collecting a precipitate, mixing with the second portion of water, adding phytase and complex enzyme, adjusting the pH to 6-6.5, stirring at 34-37° C., centrifuging, collecting a precipitate, and vacuum freeze-drying to obtain the premixed zeolite powder; The mixing comprises mixing corn starch, magnesium sulfate heptahydrate and water, stirring at 25-30° C., adjusting the pH to 4.5-5, adding pretreated zeolite powder, stirring, standing at 25-30° C., centrifuging, collecting a precipitate, and vacuum freeze-drying to obtain a mixed zeolite powder.
2. The method for preparing broiler feed for improving gizzard development according to claim 1, wherein: In the crushed corn, the moisture content of the dry corn kernels is 12-15%; In the treated corn, the weight ratio of crushed corn, chlorogenic acid, and water is 1000-1100:5-6:6000-7000; The weight ratio of crushed corn, garlic cloves, and the second portion of water is 1000-1100:25-27:2000-2500; The mass content of chlorogenic acid in the chlorogenic acid is 70%; The water content of the garlic clove is 64%; The ultrasonic power in the ultrasonic treatment is 500W and the ultrasonic frequency is 25kHz; The temperature during the vacuum freeze drying is -50°C to -45°C, and the time is 24-26 hours.
3. The method for preparing broiler feed for improving gizzard development according to claim 1, wherein: In the premix, the weight ratio of zeolite powder, tannic acid, and the first portion of water is 200-210:5-6:2000-2200; The weight ratio of zeolite powder, the second portion of water, phytase, and complex enzyme is 200-210:2000-2200:7-7.3:5.4-5.6; The average particle size of the zeolite powder is 0.8 mm; The temperature during the vacuum freeze drying is -50°C to -45°C, and the time is 30-35 hours.
4. The method for preparing broiler feed for improving gizzard development according to claim 1, wherein: The activity of the phytase is 9000-11000 U / g; The complex enzyme consists of xylanase, beta-glucanase, cellulase, amylase, protease, mannanase and glucose oxidase, wherein the activity of the xylanase is 7000-10000 U / g, the activity of the beta-glucanase is 9000-11000 U / g, the activity of the cellulase is 20-50 U / g, the activity of the amylase is 1000-3000 U / g, the activity of the protease is 900-1100 U / g, the activity of the mannanase is 1500-2500 U / g, and the activity of the glucose oxidase is 9000-11000 U / g.
5. The method for preparing broiler feed for improving gizzard development according to claim 1, wherein: In the mixture, the weight ratio of corn starch, magnesium sulfate heptahydrate, and water is 6.2-7:15-16:900-1000; The weight ratio of the corn starch in the mixture to the zeolite powder in the premix is 6.2-7:200-210; The temperature during the vacuum freeze drying is -50°C to -45°C, and the time is 30-35 hours.
6. The method for preparing broiler feed for improving gizzard development according to claim 1, wherein: The pulverizing comprises pulverizing corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine, and then passing the pulverized corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine through a 50-80 mesh sieve to obtain pulverized corn flour, soybean meal, flour, peanut meal, corn gluten meal, enzymatically hydrolyzed feather meal, monosodium glutamate residue, bone meal, L-lysine sulfate, bone dibasic calcium phosphate, sodium humate, methionine hydroxy analogue, DL-methionine, sodium chloride, L-threonine, sodium bicarbonate, choline chloride, L-arginine, and L-valine; The mass content of protein in the corn gluten meal is 60%; The mass content of lysine in the L-lysine sulfate is 65%; The mass content of sodium humate in the sodium humate is 65%; The mass content of choline chloride in the choline chloride is 70%.
7. The method for preparing broiler feed for improving gizzard development according to claim 1, wherein: The granulation method comprises the following steps: mixing, by weight, ground corn flour, ground soybean meal, ground flour, ground peanut meal, ground corn gluten meal, ground enzymatically hydrolyzed feather meal, ground monosodium glutamate residue, ground bone meal, ground L-lysine sulfate, ground bone calcium hydrogen phosphate, ground sodium humate, ground methionine hydroxy analogue, ground DL-methionine, ground sodium chloride, ground L-threonine, ground sodium bicarbonate, ground choline chloride, ground L-arginine, ground L-valine, duck fat, mixed zeolite powder, a first premix, and a second premix, followed by granulation, and drying until the moisture content is less than 12.5% to obtain pellet feed.
8. The method for preparing broiler feed for improving gizzard development according to claim 7, characterized in that: In the granulation, the crushed corn flour, the crushed soybean meal, the crushed flour, the crushed peanut meal, the crushed corn gluten meal, the crushed enzymatic hydrolyzed feather meal, the crushed monosodium glutamate residue, the crushed bone meal, the crushed L-lysine sulfate, the crushed bone calcium hydrogen phosphate, the crushed sodium humate, the crushed methionine hydroxy analogue, the crushed DL-methionine, the crushed sodium chloride, the crushed L-threonine, the crushed sodium bicarbonate, the crushed choline chloride, the crushed L-arginine, the crushed L-valine, the duck fat, the mixed zeolite powder, the first premix, The weight ratio of the second premix is 24-25:24-24.5:8-8.3:4-4.2:3-3.2:2.5-2.6:1-1.1:1-1.2:0.7-0.75:0.3-0.33:0.2-0.21:0.2-0.21:0.16-0.17:0.15-0.16:0.14-0.15:0.13-0.14:0.08-0.09:0.07-0.08:0.04-0.05:8-8.4:1.2-1.3:0.1-0.11:0.2-0.22; The first premix is a broiler chicken compound premix feed BV22 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., wherein the carrier is rice husk powder, the water content is less than 10%, the content of vitamin A acetate is 7000-10000 KIU / kg, the content of vitamin D3 is 2000-4400 KIU / kg, the content of DL-α-tocopheryl acetate is ≥25000 mg / kg, the content of ammonium sulfate nitrate is ≥2160 mg / kg, the content of menadione is ≥2160 mg / kg, the content of nicotinamide is ≥28800 mg / kg, the content of D-pantothenic acid is ≥8640 mg / kg, and the content of riboflavin is ≥6480 mg / kg. The second premix is a poultry trace element premix feed M-B2 produced by Weifang Tianpu Sunshine Feed Technology Co., Ltd., with a carrier of stone powder, a moisture content of less than 10%, a copper content of 1920-20000 mg / kg, an iron content of 19600-200000 mg / kg, a manganese content of 23200-100000 mg / kg, and a zinc content of 23600-120000 mg / kg; The diameter of the pellet feed is 3-4 mm and the length is 10-12 mm.
9. The method for preparing broiler feed for improving gizzard development according to claim 7, wherein: The mixture is prepared by uniformly mixing 24-27 parts of processed corn and 78-82 parts of pelleted feed by weight to obtain a broiler feed for improving gizzard development.
10. A broiler feed for improving gizzard development prepared by the preparation method according to any one of claims 1 to 9.
Citation Information
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